MS010808 (gene) Bitter gourd (TR) v1

Overview
NameMS010808
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionProtein PLANT CADMIUM RESISTANCE like
Locationscaffold35: 2144270 .. 2146914 (+)
RNA-Seq ExpressionMS010808
SyntenyMS010808
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGATTTGGAGAAGCAGGAGAGGGGGATTGGAAATGGGTTTGTTGGAGAAGAGGAGGAGAAGTTTCTCGAGGGGGTGACGTTACTGGATTTCGACATGTTGTGCTCGACGGTGGCGATGCAGACGCAGGGCAAATGGACCAAGTTGGAAAGCGCCGAAGATGGTGTAGCGGAACCCGAATTCGGTGGGGTTTTCAGGATGTGGGAAGGCGAACTACTCGATTGCCTCGAGGACCGCCGCATCGCTATCGAATCCACATGGTTAGCCTAACTTATCCAGTTTATAGCAGCTACGGAAAATATTTCATCTATGTGTATTGTTTCTTGAGAAATTTGTTGTGCCCCCTTCTTTTCGTTTTGCCTTTTGTTTTTTAAGGGAGGTAGGGGAATTGTTTGGGGTAGCTATCAATTTATTCTCTGGATTCTCTGTAGATGATATTTTCTTTTTTAGTTTGAAAATGTCGTTCGGACTTCAACTTTCCAGTCTAAAAAGGTCAATCAATAGTTTCTGTGCACAATTGTTCCTCATGGGTTTGGAGTATCTGTATTTGGTTGTGGTTAACTAACAGGAGATGCATATTTTGTTGTGGTTAAGTTTGGAATTATAACTATTATTGATAAATTTCTGCGGAAAAGTGGAATTTTGCTATATATAAGGTTTGTTCTCTACCTCAAAGTCAGCCACAATAGTAACATAACTTGTGTTAATGATGCAGCTGTCCGTGTCATAGATTTGGGAAGAATATGAGAAGGGCTGGTTTTGGCTCTTGTTTTCTTCAGGTAAAGATTTTTCTTGGCTGCTTGGACCTTTGAAAGTAGACGGCAAGAAGTTAGATTACAGTTTTCTTTGCTCATCAGTTTTCCTAATTTCTACTTCTTTACTTATGAAAATAATTCTATTTGCCATAATTCTGAATAACAATTTTGCAGGGAACCGTTTATTTTGCTCTTGCTCTTGGTGCCCTTTGCAACTTCATCGCCTTTTTTGTCACAAAGCGCCATTACTTTTTATATTCAGCAATTGCTTTCACCATTTCAACAGTAACGTATTTGGGTTTCTTCCGTACCCAGATGAAGAGGAAATTCAATATAAGGGTGAGAGTTCGTTTTCTATCAGCTTCTAGATGTTATCTATTTCACATTTCTACTTGCAAATTAATTGATTGTAACACATTCTATTTCAAGGATGCTGAAGTTGTTGTGCTCTAAATTGTCTTTTGCTTTTTTTGATCAATTTGTTTGTTTTCTTCTTTTTTACATCTAAGTTATAGTCAGTTCTGTTGTGGTCCCTTGTCATCTTCTGCAGACGTCTCAATTCTCATATGTTATCAACTAATGGTTTCTATTTTTCGTAGTTGCCTTAATAGGGAAAACTTAATTAAGACTGAAATTCTAAATAACGCCATCATGTTGCCATTTTGTTATGAGATTGTGCAACTGAGGAGTCGATAGTCACAAGTAGTGTTTGAAAAGCCATCAAAGTTCGAATGACTAGTATGGTTTGGATAATTTTTCTGTATCTTATAATGACTAATAGTGTGTAGACAGGCTAGTAAGAGAAAGTTTACTTACAGTCTGTGGAGAGAGACTTGTGAGAAAAAATTTACTAATAGTGTGTAGAGAGAGACTTGTAAGAGAAAGTTTACTAAAGATGTACAGAGAGAGACTGATTAGAGAAATTTTACTAAAGGTGTGCAGAGAGGGAGACTTGTAAGAGAGTTTACTAAATGTGTGCAGAGAGAGACTAGTAAGAAAGGAAGTGTGTGCGTGTTATGAGATGTTAGTGAGAAAAAAAGTGTGTGTAGAGACTAGAGAGCAAGTTACTGAGAGTAAATAGGTAGAGAGAAATTAGTGAAAGAAGATGTGGGTAGAAAGTAATTAGGGAAGGAGAGTGTTGTAAGAATAAAGTGTTTAAGACAAAAGCACTTGATAAAGTGTTTATAAATTGCTTCTTTGAAAACCACCTATTAATTGTTTGTTTCTTTGAAAAACAGTTGGAGATATTTTTGGTTTTCTGAAAAGTAATTTTAACAATTTAGAAGTACTTCTTCCATAGTAAAGCACTCCCAAACAGTTTGAGGGTGTAAAATTGGTAGAATGATGTCATTTTTGTCTCCCATTTTTTACCATGTTTGAGGCGACCATTATTTACATCCATATGTGTCTGCTTATTCATATCTGGTTTAACATCATAGCTATGCCAGCTTTCTCATTAATATTGTTAAATAAAATCTTGGCGGCGTTTTCCTCACATGCAGATTAATATTTATTTATGTTTCAGGGTGGAGATAGTTCTTTCGATGACTGCATCTACCACCTTATCTGTCCCTGCTGCGCCTTATCTCAGGTTCTGTCCTTAAAAAACTTGTTTTCATATTTAACTTCCTGATTCTGTCATGTGTAAGCAACCTAAACTAACATATTCAATTGTCATCTGATACTCGTAATCTTGAGCAGGAATCGAGAACGCTGGAAATGAACAATGTTCAAGATGGCACATGGCACGGTCGGGGCGACACAATATATATAGGTAGTTATGGTGATGCCAGCCGATTTGTTGAGCTAAACCCACCTGTTCCCATCTCAACCAAATTGCCTGAAGTTGGTACGACTCTGAAGGATGTGGAGAATTGCAACCAGTCT

mRNA sequence

ATGGCGGATTTGGAGAAGCAGGAGAGGGGGATTGGAAATGGGTTTGTTGGAGAAGAGGAGGAGAAGTTTCTCGAGGGGGTGACGTTACTGGATTTCGACATGTTGTGCTCGACGGTGGCGATGCAGACGCAGGGCAAATGGACCAAGTTGGAAAGCGCCGAAGATGGTGTAGCGGAACCCGAATTCGGTGGGGTTTTCAGGATGTGGGAAGGCGAACTACTCGATTGCCTCGAGGACCGCCGCATCGCTATCGAATCCACATGCTGTCCGTGTCATAGATTTGGGAAGAATATGAGAAGGGCTGGTTTTGGCTCTTGTTTTCTTCAGGGAACCGTTTATTTTGCTCTTGCTCTTGGTGCCCTTTGCAACTTCATCGCCTTTTTTGTCACAAAGCGCCATTACTTTTTATATTCAGCAATTGCTTTCACCATTTCAACAGTAACGTATTTGGGTTTCTTCCGTACCCAGATGAAGAGGAAATTCAATATAAGGGGTGGAGATAGTTCTTTCGATGACTGCATCTACCACCTTATCTGTCCCTGCTGCGCCTTATCTCAGCAGGAATCGAGAACGCTGGAAATGAACAATGTTCAAGATGGCACATGGCACGGTCGGGGCGACACAATATATATAGGTAGTTATGGTGATGCCAGCCGATTTGTTGAGCTAAACCCACCTGTTCCCATCTCAACCAAATTGCCTGAAGTTGGTACGACTCTGAAGGATGTGGAGAATTGCAACCAGTCT

Coding sequence (CDS)

ATGGCGGATTTGGAGAAGCAGGAGAGGGGGATTGGAAATGGGTTTGTTGGAGAAGAGGAGGAGAAGTTTCTCGAGGGGGTGACGTTACTGGATTTCGACATGTTGTGCTCGACGGTGGCGATGCAGACGCAGGGCAAATGGACCAAGTTGGAAAGCGCCGAAGATGGTGTAGCGGAACCCGAATTCGGTGGGGTTTTCAGGATGTGGGAAGGCGAACTACTCGATTGCCTCGAGGACCGCCGCATCGCTATCGAATCCACATGCTGTCCGTGTCATAGATTTGGGAAGAATATGAGAAGGGCTGGTTTTGGCTCTTGTTTTCTTCAGGGAACCGTTTATTTTGCTCTTGCTCTTGGTGCCCTTTGCAACTTCATCGCCTTTTTTGTCACAAAGCGCCATTACTTTTTATATTCAGCAATTGCTTTCACCATTTCAACAGTAACGTATTTGGGTTTCTTCCGTACCCAGATGAAGAGGAAATTCAATATAAGGGGTGGAGATAGTTCTTTCGATGACTGCATCTACCACCTTATCTGTCCCTGCTGCGCCTTATCTCAGCAGGAATCGAGAACGCTGGAAATGAACAATGTTCAAGATGGCACATGGCACGGTCGGGGCGACACAATATATATAGGTAGTTATGGTGATGCCAGCCGATTTGTTGAGCTAAACCCACCTGTTCCCATCTCAACCAAATTGCCTGAAGTTGGTACGACTCTGAAGGATGTGGAGAATTGCAACCAGTCT

Protein sequence

MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEPEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTLKDVENCNQS
Homology
BLAST of MS010808 vs. NCBI nr
Match: XP_022142029.1 (protein PLANT CADMIUM RESISTANCE 11 [Momordica charantia])

HSP 1 Score: 515.4 bits (1326), Expect = 2.8e-142
Identity = 248/249 (99.60%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP
Sbjct: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA
Sbjct: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP
Sbjct: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240

Query: 241 KDVENCNQS 250
           KDVENCNQS
Sbjct: 241 KDVENCNQS 248

BLAST of MS010808 vs. NCBI nr
Match: XP_038883663.1 (protein PLANT CADMIUM RESISTANCE 11 [Benincasa hispida])

HSP 1 Score: 436.4 bits (1121), Expect = 1.6e-118
Identity = 210/250 (84.00%), Postives = 222/250 (88.80%), Query Frame = 0

Query: 1   MADLEKQER-GIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAE 60
           MADLEKQ+R  +GNGF+G+EEEK L+GVTLLDFDMLCSTVAMQTQGKW KLE+ ED    
Sbjct: 1   MADLEKQDRVVVGNGFLGDEEEKLLDGVTLLDFDMLCSTVAMQTQGKWAKLETTEDAATG 60

Query: 61  PEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALG 120
           PEFGGVFRMWEGELLDC +DRRIAIES CCPCHRFGKNM RAGFGSCFLQGTVY  L LG
Sbjct: 61  PEFGGVFRMWEGELLDCYDDRRIAIESACCPCHRFGKNMGRAGFGSCFLQGTVYLILVLG 120

Query: 121 ALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLIC 180
           ALCNFIAF V+KRHYFLYSAIAFTISTVTYLGFFRTQM+RKFNIRGGD+S DDCIYHLIC
Sbjct: 121 ALCNFIAFLVSKRHYFLYSAIAFTISTVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLIC 180

Query: 181 PCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTT 240
           PCCALS QESRTLEMNNVQDGTWHGRGDTI IGSYGDASR VELNPP P+STK+PE    
Sbjct: 181 PCCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGDASRIVELNPPPPVSTKMPEPCGI 240

Query: 241 LKDVENCNQS 250
           LKDV N N S
Sbjct: 241 LKDVANHNHS 249

BLAST of MS010808 vs. NCBI nr
Match: XP_008439930.1 (PREDICTED: uncharacterized protein LOC103484568 [Cucumis melo])

HSP 1 Score: 433.7 bits (1114), Expect = 1.1e-117
Identity = 209/250 (83.60%), Postives = 220/250 (88.00%), Query Frame = 0

Query: 1   MADLEKQERGI-GNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAE 60
           MADLEK +R + GN F+  EE+K L+GVTLLDFDMLCSTVAMQTQGKW KLE+ ED    
Sbjct: 1   MADLEKHDRVVGGNAFLSAEEDKLLDGVTLLDFDMLCSTVAMQTQGKWAKLETTEDATTG 60

Query: 61  PEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALG 120
           PEFGGVFRMWEGELLDC +DRRIAIES CCPCHRFGKNM RAGFGSCFLQGTVY  LALG
Sbjct: 61  PEFGGVFRMWEGELLDCYDDRRIAIESACCPCHRFGKNMGRAGFGSCFLQGTVYLVLALG 120

Query: 121 ALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLIC 180
           ALC FIAF VTKRHYFLYSAIAFTISTVTYLGFFRTQM+RKFNIRGGD+S DDCIYHLIC
Sbjct: 121 ALCTFIAFLVTKRHYFLYSAIAFTISTVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLIC 180

Query: 181 PCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTT 240
           PCCALS QESRTLEMNNVQDGTWHGRGDTI IGSYGD SRFVELNPP+P+STKLPE   T
Sbjct: 181 PCCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGDPSRFVELNPPLPVSTKLPEACGT 240

Query: 241 LKDVENCNQS 250
           LKDV N N S
Sbjct: 241 LKDVANENHS 249

BLAST of MS010808 vs. NCBI nr
Match: XP_022950802.1 (protein PLANT CADMIUM RESISTANCE 10-like [Cucurbita moschata])

HSP 1 Score: 427.6 bits (1098), Expect = 7.6e-116
Identity = 206/249 (82.73%), Postives = 218/249 (87.55%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLE Q+RG+GN  VGEEEEK LEGVTL+DFDMLCSTVAMQTQGKW KLE+  D   EP
Sbjct: 1   MADLENQDRGLGNVVVGEEEEKLLEGVTLVDFDMLCSTVAMQTQGKWAKLETTHDAPMEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           + GGVFRMWEGELLDC +DRRIAIESTC PCHRFGKNM RAGFGSCFLQGTVY +LALGA
Sbjct: 61  DLGGVFRMWEGELLDCFDDRRIAIESTCWPCHRFGKNMARAGFGSCFLQGTVYLSLALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAF VTKRHYFLY AIAFTIS VTYLGFFRTQM+RKFNIRGGD+S DDCIYHLICP
Sbjct: 121 LCNFIAFLVTKRHYFLYPAIAFTISAVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTI IGSYG+ASRFVELNPP P+STK  E    L
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGEASRFVELNPPDPVSTKSSEACGIL 240

Query: 241 KDVENCNQS 250
           KDV N + S
Sbjct: 241 KDVANHDHS 248

BLAST of MS010808 vs. NCBI nr
Match: XP_023544493.1 (uncharacterized protein LOC111804053 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 427.2 bits (1097), Expect = 9.9e-116
Identity = 206/249 (82.73%), Postives = 218/249 (87.55%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLE Q+RG+GN  VGEEEEK LEGVTL+DFDMLCSTVAMQTQGKW KLE+  D   EP
Sbjct: 1   MADLENQDRGLGNVVVGEEEEKLLEGVTLVDFDMLCSTVAMQTQGKWAKLETTRDASMEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           + GGVFRMWEGELLDC +DRRIAIESTC PCHRFGKNM RAGFGSCFLQGTVY +LALGA
Sbjct: 61  DLGGVFRMWEGELLDCFDDRRIAIESTCWPCHRFGKNMGRAGFGSCFLQGTVYLSLALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAF VTKRHYFLY AIAFTIS VTYLGFFRTQM+RKFNIRGGD+S DDCIYHLICP
Sbjct: 121 LCNFIAFLVTKRHYFLYPAIAFTISAVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTI IGSYG+ASRFVELNPP P+STK  E    L
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGEASRFVELNPPDPVSTKSSEACGIL 240

Query: 241 KDVENCNQS 250
           KDV N + S
Sbjct: 241 KDVANHDHS 248

BLAST of MS010808 vs. ExPASy Swiss-Prot
Match: Q9M815 (Protein PLANT CADMIUM RESISTANCE 8 OS=Arabidopsis thaliana OX=3702 GN=PCR8 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 3.8e-09
Identity = 45/143 (31.47%), Postives = 70/143 (48.95%), Query Frame = 0

Query: 65  VFRMWEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALC 124
           V R W   L DC  D+  A+ +T  PC  FG+   +   G  +C L GT  + L + ALC
Sbjct: 49  VGRPWSTGLFDCQADQANAVLTTIVPCVTFGQIAEVMDEGEMTCPL-GTFMYLLMMPALC 108

Query: 125 NFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCC 184
           +         H+ + S              +R +M+RKFN+   ++ + DC  H++CPCC
Sbjct: 109 S---------HWVMGSK-------------YREKMRRKFNL--VEAPYSDCASHVLCPCC 165

Query: 185 ALSQQESRTLEMNNVQDGT-WHG 205
           +L  QE R L++ N+     W+G
Sbjct: 169 SLC-QEYRELKIRNLDPSLGWNG 165

BLAST of MS010808 vs. ExPASy Swiss-Prot
Match: Q9LQU2 (Protein PLANT CADMIUM RESISTANCE 1 OS=Arabidopsis thaliana OX=3702 GN=PCR1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 33/121 (27.27%), Postives = 48/121 (39.67%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALCNFIA 128
           W     DC  D R    + CCPC  FG+   +   G  SC   G +Y  + L   C  + 
Sbjct: 15  WSTGFCDCFSDCRNCCITLCCPCITFGQVAEIVDRGSKSCCAAGALYMLIDLITSCGRM- 74

Query: 129 FFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQ 188
                                 Y  F+  +M+ ++NI+G   +  DC+ H  C  CAL+Q
Sbjct: 75  ----------------------YACFYSGKMRAQYNIKGDGCT--DCLKHFCCNLCALTQ 110

BLAST of MS010808 vs. ExPASy Swiss-Prot
Match: Q3EBY6 (Protein MID1-COMPLEMENTING ACTIVITY 2 OS=Arabidopsis thaliana OX=3702 GN=MCA2 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-04
Identity = 40/160 (25.00%), Postives = 64/160 (40.00%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGALCNFIAFF 128
           W  +LLDC  +  + +++   PC    K                   ++  A    I+  
Sbjct: 292 WHADLLDCCSEPCLCLKTLFFPCGTLAK-------------------ISTVATSRQISST 351

Query: 129 VTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQQE 188
              ++  +YS I   +S   Y    R ++++  NI GG    DD + HL+C CCAL Q  
Sbjct: 352 EVCKNLIVYSLI---LSCCCYTCCIRKKLRKTLNITGG--CIDDFLSHLMCCCCALVQ-- 411

Query: 189 SRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVP 229
               E+  V+          I+  SYG      E++PP P
Sbjct: 412 ----ELREVE----------IHRASYGTEKSNKEMSPPTP 411

BLAST of MS010808 vs. ExPASy Swiss-Prot
Match: P0CW97 (Protein PLANT CADMIUM RESISTANCE 3 OS=Arabidopsis thaliana OX=3702 GN=PCR3 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 4.8e-04
Identity = 34/121 (28.10%), Postives = 50/121 (41.32%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALCNFIA 128
           W     DC  D +    +  CPC  FG+  ++   G  SC   G +Y  LA    C    
Sbjct: 16  WSTGFCDCFSDCQNCCITWLCPCITFGQVADIVDRGNTSCGTAGALYVLLAAITGCG--- 75

Query: 129 FFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQ 188
                    LYS I            +R +++ ++NIRG   +  DC+ H  C  CAL+Q
Sbjct: 76  --------CLYSCI------------YRGKIRAQYNIRGDGCT--DCLKHFCCELCALTQ 111

BLAST of MS010808 vs. ExPASy TrEMBL
Match: A0A6J1CKE9 (protein PLANT CADMIUM RESISTANCE 11 OS=Momordica charantia OX=3673 GN=LOC111012258 PE=4 SV=1)

HSP 1 Score: 515.4 bits (1326), Expect = 1.3e-142
Identity = 248/249 (99.60%), Postives = 248/249 (99.60%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP
Sbjct: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA
Sbjct: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP
Sbjct: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240

Query: 241 KDVENCNQS 250
           KDVENCNQS
Sbjct: 241 KDVENCNQS 248

BLAST of MS010808 vs. ExPASy TrEMBL
Match: A0A1S3AZI4 (uncharacterized protein LOC103484568 OS=Cucumis melo OX=3656 GN=LOC103484568 PE=4 SV=1)

HSP 1 Score: 433.7 bits (1114), Expect = 5.1e-118
Identity = 209/250 (83.60%), Postives = 220/250 (88.00%), Query Frame = 0

Query: 1   MADLEKQERGI-GNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAE 60
           MADLEK +R + GN F+  EE+K L+GVTLLDFDMLCSTVAMQTQGKW KLE+ ED    
Sbjct: 1   MADLEKHDRVVGGNAFLSAEEDKLLDGVTLLDFDMLCSTVAMQTQGKWAKLETTEDATTG 60

Query: 61  PEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALG 120
           PEFGGVFRMWEGELLDC +DRRIAIES CCPCHRFGKNM RAGFGSCFLQGTVY  LALG
Sbjct: 61  PEFGGVFRMWEGELLDCYDDRRIAIESACCPCHRFGKNMGRAGFGSCFLQGTVYLVLALG 120

Query: 121 ALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLIC 180
           ALC FIAF VTKRHYFLYSAIAFTISTVTYLGFFRTQM+RKFNIRGGD+S DDCIYHLIC
Sbjct: 121 ALCTFIAFLVTKRHYFLYSAIAFTISTVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLIC 180

Query: 181 PCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTT 240
           PCCALS QESRTLEMNNVQDGTWHGRGDTI IGSYGD SRFVELNPP+P+STKLPE   T
Sbjct: 181 PCCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGDPSRFVELNPPLPVSTKLPEACGT 240

Query: 241 LKDVENCNQS 250
           LKDV N N S
Sbjct: 241 LKDVANENHS 249

BLAST of MS010808 vs. ExPASy TrEMBL
Match: A0A6J1GFW1 (protein PLANT CADMIUM RESISTANCE 10-like OS=Cucurbita moschata OX=3662 GN=LOC111453800 PE=4 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.7e-116
Identity = 206/249 (82.73%), Postives = 218/249 (87.55%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLE Q+RG+GN  VGEEEEK LEGVTL+DFDMLCSTVAMQTQGKW KLE+  D   EP
Sbjct: 1   MADLENQDRGLGNVVVGEEEEKLLEGVTLVDFDMLCSTVAMQTQGKWAKLETTHDAPMEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           + GGVFRMWEGELLDC +DRRIAIESTC PCHRFGKNM RAGFGSCFLQGTVY +LALGA
Sbjct: 61  DLGGVFRMWEGELLDCFDDRRIAIESTCWPCHRFGKNMARAGFGSCFLQGTVYLSLALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAF VTKRHYFLY AIAFTIS VTYLGFFRTQM+RKFNIRGGD+S DDCIYHLICP
Sbjct: 121 LCNFIAFLVTKRHYFLYPAIAFTISAVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTI IGSYG+ASRFVELNPP P+STK  E    L
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGEASRFVELNPPDPVSTKSSEACGIL 240

Query: 241 KDVENCNQS 250
           KDV N + S
Sbjct: 241 KDVANHDHS 248

BLAST of MS010808 vs. ExPASy TrEMBL
Match: A0A0A0KN60 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G516790 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.1e-115
Identity = 206/251 (82.07%), Postives = 221/251 (88.05%), Query Frame = 0

Query: 1   MAD-LEKQER-GIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVA 60
           MAD ++K +R  +GN F+  EE+K L+GVTLLDFDMLCSTVAMQTQGKW  L++ +D   
Sbjct: 1   MADSVDKHDRVVVGNAFLAPEEDKLLDGVTLLDFDMLCSTVAMQTQGKWPNLDTTQDPAT 60

Query: 61  EPEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALAL 120
            PEFGGVFRMWEGELLDC +DRRIAIES CCPCHRFGKNM RAGFGSCFLQGTVY ALAL
Sbjct: 61  APEFGGVFRMWEGELLDCYDDRRIAIESACCPCHRFGKNMGRAGFGSCFLQGTVYLALAL 120

Query: 121 GALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLI 180
           GALCNFIAF VTK HYFLYSAIAFTISTVTYLGFFRTQM+RKFNIRGGD+S DDCIYHLI
Sbjct: 121 GALCNFIAFLVTKHHYFLYSAIAFTISTVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLI 180

Query: 181 CPCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGT 240
           CPCCALS QESRTLEMNNVQDGTWHGRGDTI IGSYGDASRFVELNPP+P+STKLPE   
Sbjct: 181 CPCCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGDASRFVELNPPLPVSTKLPEACG 240

Query: 241 TLKDVENCNQS 250
           TLKDV N N S
Sbjct: 241 TLKDVANQNHS 250

BLAST of MS010808 vs. ExPASy TrEMBL
Match: A0A6J1II90 (protein PLANT CADMIUM RESISTANCE 10-like OS=Cucurbita maxima OX=3661 GN=LOC111477715 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.5e-114
Identity = 204/249 (81.93%), Postives = 216/249 (86.75%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFVGEEEEKFLEGVTLLDFDMLCSTVAMQTQGKWTKLESAEDGVAEP 60
           MADLE Q+ G+GN  VGEEEEK LEGVTL+DFDMLCSTVAMQTQGKW KLE+  D   EP
Sbjct: 1   MADLENQDTGLGNVVVGEEEEKLLEGVTLVDFDMLCSTVAMQTQGKWAKLETTRDAPMEP 60

Query: 61  EFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGA 120
           + GGVFRMWEGELLDC +DRRIAIESTC PCHRFGKNM RAGFGSCFLQGTVY +LALGA
Sbjct: 61  DLGGVFRMWEGELLDCFDDRRIAIESTCWPCHRFGKNMARAGFGSCFLQGTVYLSLALGA 120

Query: 121 LCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICP 180
           LCNFIAF VTKRHYFLY AIAFTIS VTYLGFFRTQM+RKFNIRGGD+S DDCIYHLICP
Sbjct: 121 LCNFIAFLVTKRHYFLYPAIAFTISAVTYLGFFRTQMRRKFNIRGGDNSLDDCIYHLICP 180

Query: 181 CCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVPISTKLPEVGTTL 240
           CCALS QESRTLEMNNVQDGTWHGRGDTI IGSYG+ASRFVELN P P+STK  E    L
Sbjct: 181 CCALS-QESRTLEMNNVQDGTWHGRGDTICIGSYGEASRFVELNKPDPVSTKSSEACGIL 240

Query: 241 KDVENCNQS 250
           KDV N + S
Sbjct: 241 KDVANHDHS 248

BLAST of MS010808 vs. TAIR 10
Match: AT1G11380.1 (PLAC8 family protein )

HSP 1 Score: 285.4 bits (729), Expect = 4.3e-77
Identity = 146/249 (58.63%), Postives = 175/249 (70.28%), Query Frame = 0

Query: 1   MADLEKQERGIGNGFV-------------GEEEEKF--LEGVTLLDFDMLCSTVAMQTQG 60
           MADL+K E G G G V              EE E+   LEG+++LDFD+LCSTVA+QTQG
Sbjct: 1   MADLDKLEGGGGGGAVVIADIRADEGGGAVEERERLTELEGISVLDFDLLCSTVALQTQG 60

Query: 61  KWTKLESA--EDGVAEPEFGGVFRMWEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGF 120
           KW KLES+  ED   +   GGV R+WEG+++DC EDR + IES CCPC+RFGKNM R GF
Sbjct: 61  KWRKLESSEGEDATEDDYGGGVLRLWEGDVMDCFEDRHLCIESACCPCYRFGKNMTRTGF 120

Query: 121 GSCFLQGTVYFALALGALCNFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNI 180
           GSCFLQG V+  L  G L N +AF VTKRH FLY AIAF +   +YLGFFR  ++RKFNI
Sbjct: 121 GSCFLQGAVHMILIAGFLFNVVAFAVTKRHCFLYLAIAFVLLIGSYLGFFRMLIRRKFNI 180

Query: 181 RGGDSSFDDCIYHLICPCCALSQQESRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVEL 232
           RG DS  DD I+HL+CP C L+ QES+TLEMNNV DG WHGRGDT+ IG Y +   F+EL
Sbjct: 181 RGTDSFLDDFIHHLVCPFCTLT-QESKTLEMNNVHDGIWHGRGDTLCIGGYSEGKAFLEL 240

BLAST of MS010808 vs. TAIR 10
Match: AT1G52200.1 (PLAC8 family protein )

HSP 1 Score: 63.5 bits (153), Expect = 2.7e-10
Identity = 45/143 (31.47%), Postives = 70/143 (48.95%), Query Frame = 0

Query: 65  VFRMWEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALC 124
           V R W   L DC  D+  A+ +T  PC  FG+   +   G  +C L GT  + L + ALC
Sbjct: 49  VGRPWSTGLFDCQADQANAVLTTIVPCVTFGQIAEVMDEGEMTCPL-GTFMYLLMMPALC 108

Query: 125 NFIAFFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCC 184
           +         H+ + S              +R +M+RKFN+   ++ + DC  H++CPCC
Sbjct: 109 S---------HWVMGSK-------------YREKMRRKFNL--VEAPYSDCASHVLCPCC 165

Query: 185 ALSQQESRTLEMNNVQDGT-WHG 205
           +L  QE R L++ N+     W+G
Sbjct: 169 SLC-QEYRELKIRNLDPSLGWNG 165

BLAST of MS010808 vs. TAIR 10
Match: AT1G14880.1 (PLANT CADMIUM RESISTANCE 1 )

HSP 1 Score: 52.0 bits (123), Expect = 8.1e-07
Identity = 33/121 (27.27%), Postives = 48/121 (39.67%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALCNFIA 128
           W     DC  D R    + CCPC  FG+   +   G  SC   G +Y  + L   C  + 
Sbjct: 15  WSTGFCDCFSDCRNCCITLCCPCITFGQVAEIVDRGSKSCCAAGALYMLIDLITSCGRM- 74

Query: 129 FFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQ 188
                                 Y  F+  +M+ ++NI+G   +  DC+ H  C  CAL+Q
Sbjct: 75  ----------------------YACFYSGKMRAQYNIKGDGCT--DCLKHFCCNLCALTQ 110

BLAST of MS010808 vs. TAIR 10
Match: AT2G17780.1 (PLAC8 family protein )

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-05
Identity = 40/160 (25.00%), Postives = 64/160 (40.00%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGKNMRRAGFGSCFLQGTVYFALALGALCNFIAFF 128
           W  +LLDC  +  + +++   PC    K                   ++  A    I+  
Sbjct: 292 WHADLLDCCSEPCLCLKTLFFPCGTLAK-------------------ISTVATSRQISST 351

Query: 129 VTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQQE 188
              ++  +YS I   +S   Y    R ++++  NI GG    DD + HL+C CCAL Q  
Sbjct: 352 EVCKNLIVYSLI---LSCCCYTCCIRKKLRKTLNITGG--CIDDFLSHLMCCCCALVQ-- 411

Query: 189 SRTLEMNNVQDGTWHGRGDTIYIGSYGDASRFVELNPPVP 229
               E+  V+          I+  SYG      E++PP P
Sbjct: 412 ----ELREVE----------IHRASYGTEKSNKEMSPPTP 411

BLAST of MS010808 vs. TAIR 10
Match: AT5G35525.1 (PLAC8 family protein )

HSP 1 Score: 46.6 bits (109), Expect = 3.4e-05
Identity = 34/121 (28.10%), Postives = 50/121 (41.32%), Query Frame = 0

Query: 69  WEGELLDCLEDRRIAIESTCCPCHRFGK--NMRRAGFGSCFLQGTVYFALALGALCNFIA 128
           W     DC  D +    +  CPC  FG+  ++   G  SC   G +Y  LA    C    
Sbjct: 16  WSTGFCDCFSDCQNCCITWLCPCITFGQVADIVDRGNTSCGTAGALYVLLAAITGCG--- 75

Query: 129 FFVTKRHYFLYSAIAFTISTVTYLGFFRTQMKRKFNIRGGDSSFDDCIYHLICPCCALSQ 188
                    LYS I            +R +++ ++NIRG   +  DC+ H  C  CAL+Q
Sbjct: 76  --------CLYSCI------------YRGKIRAQYNIRGDGCT--DCLKHFCCELCALTQ 111

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022142029.12.8e-14299.60protein PLANT CADMIUM RESISTANCE 11 [Momordica charantia][more]
XP_038883663.11.6e-11884.00protein PLANT CADMIUM RESISTANCE 11 [Benincasa hispida][more]
XP_008439930.11.1e-11783.60PREDICTED: uncharacterized protein LOC103484568 [Cucumis melo][more]
XP_022950802.17.6e-11682.73protein PLANT CADMIUM RESISTANCE 10-like [Cucurbita moschata][more]
XP_023544493.19.9e-11682.73uncharacterized protein LOC111804053 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9M8153.8e-0931.47Protein PLANT CADMIUM RESISTANCE 8 OS=Arabidopsis thaliana OX=3702 GN=PCR8 PE=1 ... [more]
Q9LQU21.1e-0527.27Protein PLANT CADMIUM RESISTANCE 1 OS=Arabidopsis thaliana OX=3702 GN=PCR1 PE=1 ... [more]
Q3EBY61.6e-0425.00Protein MID1-COMPLEMENTING ACTIVITY 2 OS=Arabidopsis thaliana OX=3702 GN=MCA2 PE... [more]
P0CW974.8e-0428.10Protein PLANT CADMIUM RESISTANCE 3 OS=Arabidopsis thaliana OX=3702 GN=PCR3 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1CKE91.3e-14299.60protein PLANT CADMIUM RESISTANCE 11 OS=Momordica charantia OX=3673 GN=LOC1110122... [more]
A0A1S3AZI45.1e-11883.60uncharacterized protein LOC103484568 OS=Cucumis melo OX=3656 GN=LOC103484568 PE=... [more]
A0A6J1GFW13.7e-11682.73protein PLANT CADMIUM RESISTANCE 10-like OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A0A0KN601.1e-11582.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G516790 PE=4 SV=1[more]
A0A6J1II901.5e-11481.93protein PLANT CADMIUM RESISTANCE 10-like OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
Match NameE-valueIdentityDescription
AT1G11380.14.3e-7758.63PLAC8 family protein [more]
AT1G52200.12.7e-1031.47PLAC8 family protein [more]
AT1G14880.18.1e-0727.27PLANT CADMIUM RESISTANCE 1 [more]
AT2G17780.11.2e-0525.00PLAC8 family protein [more]
AT5G35525.13.4e-0528.10PLAC8 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006461PLAC8 motif-containing proteinTIGRFAMTIGR01571TIGR01571coord: 68..194
e-value: 3.5E-27
score: 93.2
IPR006461PLAC8 motif-containing proteinPFAMPF04749PLAC8coord: 69..188
e-value: 5.1E-18
score: 65.7
IPR006461PLAC8 motif-containing proteinPANTHERPTHR15907DUF614 FAMILY PROTEIN-RELATEDcoord: 18..214
NoneNo IPR availablePANTHERPTHR15907:SF118DUF614 FAMILY PROTEINcoord: 18..214

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010808.1MS010808.1mRNA