MS010768 (gene) Bitter gourd (TR) v1

Overview


Name	MS010768
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Lactoylglutathione lyase
Location	scaffold35: 1864195 .. 1866703 (+)
RNA-Seq Expression	MS010768
Synteny	MS010768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCTTGCTCCTAATGCCGAGTTGTTGGATTGGCCCAGGAAAGACAAGCGCCGGTTTCTTCATGTCGTGTACCGCGTTGGTGACCTTGACCGCAGTATCAAGTATGATGCTCTTCTTTGGCGCAAGAATTTCTGAATTTCGCGTTTGAGTGGTTTGATTATTTGTTTCCAGGTTTTATACTGAATGTTTTGGGATGAAATTGTTGAGGAAGAGGGATGTGCCAGAAGAGAAATATTCTAATGCCTTCCTCGGATTTGGCCCAGAAGTGTCTAATTTTGTGGTGGAGTTGACATATAGTATGTACCTCATATTTATCCCTCATGTCCGTTTCAACACGCTCTTGAATATCTGTGATTATGTTCTCGGATTAAGCGTGATAGACTCTAGCTTGAAGTCAACGCGAATGGTTCTAATATCTGAAGGAACAGCGGGCAGAAAACTTAAAATTGTGGCTTTAATTTATTTTCTGGAATATATTAGCTTATTCTAAGCAGAAGCAGTTGATTGGTGAAAGTATCATCACCATCAATTTCTGTGTTATTCCACAACACCTTTTCTGCTGGTTTCTACCCAGAGAAGTTATCTTTGCTTCCTCCACCTAGTCGTTGAAGTTTGAACATGATGAGTAGATCTAAGTTCTCAACTTTTCTTTACCAGAGTTGTTTGCAATTTTTACTATATTAATTTGATGATAAACTTAGATGTCTATGGTATGTCATATTTTTTTATATAATTTGAATTATTTTAGTATTGATTTACTTCTTATTAGGTATCCAATTATGATTTGGATATAATTTAGATTTCAAATTCCTTGTATCTAAATTTATTTGATTAAGATATTACTTGTTTTAGTTGTCTTAATCCTATTATAAATGGATGTGTAGGGTTGTATCCAATTTGACGATTTGAATCAATAAAATTGTTTTGTTTATTTTACCTATTTTCTCTCCTCCAAAATTTATCACATATGGATGGTGTTTTGCAGATTATGGAGTCAGTTCATATGATATCGGGACAGGCTTTGGCCACCTTGCTATTGCAACTCAAGATGTAAGTATGTCTCTTAATAGTAAGTTAATTTGTTTTCCCTTTAGCTGAAACTGACCTCTCTTAGACTTGTAGGTATATAAACTCGTAGAAGATATTCGAGCCAAGGGTGGGAGCATTATCAAGGAGCCCATGGTCCTAGGAGGATCCACTGTCCTTGCCTTTCTAAAGGATCCTGATGGTTATATTTTTGAACTCACCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTACTGCTTCGTGTTGGTGATCTCGAACGCTCTATAAAGTTTTACGAGAAGGTATCCCATTCTAAGGAATTTGTTTGTCTGTGTTTGATATCCTAGCCATTTACTGGAGGGACTATTTAAGAGAGATATTCTTGAACGATGACATGTAATTTATTTTGACATGTGTAATGAATACTTGTACCAAGACTGGCTTAATTGTTCTGAGATTTATTGGTGATAGAGTGCACTTCATATTTTAGTGTACCTGTATTTTATGGGTTTTCTTTGCGTAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGACATGCCAGAATACAAGGTTAACTAGATTCTTGTTTTGATAGTGACCACCTATTCTTTGCCTACGAGTTTATCTTTCATTCTTCTGATTCTGGAAATGCTTTTGGATCTTTCTATTTTGCTATATGCGTTCTGCAGTATACTGTAGCCGTGATGGGATATGCAGGTGAATTAGAGGCAACAGCGCTGGAGTTGGCTTATAATTATGGTGTGACTGAGTACACCAAGGGAACCGCATATGCTCAGGTGAGCTTATTGTACACCTTTTCTCTATCAGTTATTACTCATTAAACAATCGATTTCGTATTTGTTTCAGATTGCCATCAGTACCGATGATGTATACAAGAGTGGTGAGGTTGTCAAACAGGTTACTGAAGAACTTGGAGGAAAGATAACCCGAGAACCAGGACCACTTCCCGGACTCGGCACAAAAATTGTTTCCTTTTTGGATCCTGATGGTTGGAAGACTGTAAGTATCGTATTGTAATGGCTCTACATTCAATTTGGTCTTAACATTATTCATTCAGCAAAAATTTTCTGGTTTACATTCAATGTTCAAAAAGAAGAAAATGGAAGAAATTAATTATAGTTTACGTTTGGGTGGGAAGATGGGACTGCAATCAGATATCTGTTGCTTCTGGACAGTCTTCCGTTACAAGAATACAAGTTGGACTACCTCTAATTGTTGTTTTACATGTTCCACATGTATACTGTTTTGAGATTTGCTTCAAGAATGATAATTTGTCAGTGCAATAACCTGTTTGTTTTAGTTTCAAAATAGTTCACCTCTGACTGGTTTGCAACTTGAAATCCAACTTGTTTGTTGGCTATGACATCTGAATTGACTTTGTAATTTAGTTATGGCTAAACTTTTTGTTGTTGTTGTAGGTCCTTGTTGATAATGTAGATTTCCTGAAGGAACTGGAA

mRNA sequence

TCTCTTGCTCCTAATGCCGAGTTGTTGGATTGGCCCAGGAAAGACAAGCGCCGGTTTCTTCATGTCGTGTACCGCGTTGGTGACCTTGACCGCAGTATCAAGTTTTATACTGAATGTTTTGGGATGAAATTGTTGAGGAAGAGGGATGTGCCAGAAGAGAAATATTCTAATGCCTTCCTCGGATTTGGCCCAGAAGTGTCTAATTTTGTGGTGGAGTTGACATATAATTATGGAGTCAGTTCATATGATATCGGGACAGGCTTTGGCCACCTTGCTATTGCAACTCAAGATGTATATAAACTCGTAGAAGATATTCGAGCCAAGGGTGGGAGCATTATCAAGGAGCCCATGGTCCTAGGAGGATCCACTGTCCTTGCCTTTCTAAAGGATCCTGATGGTTATATTTTTGAACTCACCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTACTGCTTCGTGTTGGTGATCTCGAACGCTCTATAAAGTTTTACGAGAAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGACATGCCAGAATACAAGTATACTGTAGCCGTGATGGGATATGCAGGTGAATTAGAGGCAACAGCGCTGGAGTTGGCTTATAATTATGGTGTGACTGAGTACACCAAGGGAACCGCATATGCTCAGATTGCCATCAGTACCGATGATGTATACAAGAGTGGTGAGGTTGTCAAACAGGTTACTGAAGAACTTGGAGGAAAGATAACCCGAGAACCAGGACCACTTCCCGGACTCGGCACAAAAATTGTTTCCTTTTTGGATCCTGATGGTTGGAAGACTGTCCTTGTTGATAATGTAGATTTCCTGAAGGAACTGGAA

Coding sequence (CDS)

TCTCTTGCTCCTAATGCCGAGTTGTTGGATTGGCCCAGGAAAGACAAGCGCCGGTTTCTTCATGTCGTGTACCGCGTTGGTGACCTTGACCGCAGTATCAAGTTTTATACTGAATGTTTTGGGATGAAATTGTTGAGGAAGAGGGATGTGCCAGAAGAGAAATATTCTAATGCCTTCCTCGGATTTGGCCCAGAAGTGTCTAATTTTGTGGTGGAGTTGACATATAATTATGGAGTCAGTTCATATGATATCGGGACAGGCTTTGGCCACCTTGCTATTGCAACTCAAGATGTATATAAACTCGTAGAAGATATTCGAGCCAAGGGTGGGAGCATTATCAAGGAGCCCATGGTCCTAGGAGGATCCACTGTCCTTGCCTTTCTAAAGGATCCTGATGGTTATATTTTTGAACTCACCCAAAGAGGTCCTACTCCTGAGCCACTCTGTCAAGTACTGCTTCGTGTTGGTGATCTCGAACGCTCTATAAAGTTTTACGAGAAGGCCTTGGGAATGAGGCTATTGAAGAAGGTTGACATGCCAGAATACAAGTATACTGTAGCCGTGATGGGATATGCAGGTGAATTAGAGGCAACAGCGCTGGAGTTGGCTTATAATTATGGTGTGACTGAGTACACCAAGGGAACCGCATATGCTCAGATTGCCATCAGTACCGATGATGTATACAAGAGTGGTGAGGTTGTCAAACAGGTTACTGAAGAACTTGGAGGAAAGATAACCCGAGAACCAGGACCACTTCCCGGACTCGGCACAAAAATTGTTTCCTTTTTGGATCCTGATGGTTGGAAGACTGTCCTTGTTGATAATGTAGATTTCCTGAAGGAACTGGAA

Protein sequence

SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLGGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMPEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE

Homology

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BLAST of MS010768 vs. NCBI nr
Match: XP_022142108.1 (putative lactoylglutathione lyase isoform X1 [Momordica charantia])

HSP 1 Score: 576.2 bits (1484), Expect = 1.5e-160
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 6   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 65

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG 120
           GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG
Sbjct: 66  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG 125

Query: 121 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 180
           GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP
Sbjct: 126 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 185

Query: 181 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 240
           EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE
Sbjct: 186 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 245

Query: 241 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE
Sbjct: 246 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 288

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BLAST of MS010768 vs. NCBI nr
Match: TYK13040.1 (putative lactoylglutathione lyase [Cucumis melo var. makuwa])

HSP 1 Score: 519.2 bits (1336), Expect = 2.2e-143
Identity = 251/284 (88.38%), Postives = 263/284 (92.61%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWP+KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 7   SLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 66

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYGVSSYDIGTGFGH AIATQDVYKLVEDIRAKGG I +EP  V 
Sbjct: 67  GFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITREPGPVK 126

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 127 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 186

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AI TDDVYKS EVV QV +
Sbjct: 187 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVVNQVNQ 246

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+PGPLPG+GTKIV FLDPDGWKTVLVDN DFLKELE
Sbjct: 247 ELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELE 290

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BLAST of MS010768 vs. NCBI nr
Match: XP_008440010.1 (PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >XP_008440011.1 PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >XP_008440014.1 PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >XP_016899289.1 PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >XP_016899290.1 PREDICTED: putative lactoylglutathione lyase [Cucumis melo])

HSP 1 Score: 519.2 bits (1336), Expect = 2.2e-143
Identity = 251/284 (88.38%), Postives = 263/284 (92.61%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWP+KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 7   SLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 66

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYGVSSYDIGTGFGH AIATQDVYKLVEDIRAKGG I +EP  V 
Sbjct: 67  GFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITREPGPVK 126

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 127 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 186

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AI TDDVYKS EVV QV +
Sbjct: 187 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVVNQVNQ 246

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+PGPLPG+GTKIV FLDPDGWKTVLVDN DFLKELE
Sbjct: 247 ELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELE 290

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BLAST of MS010768 vs. NCBI nr
Match: XP_038882184.1 (lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882185.1 lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882186.1 lactoylglutathione lyase GLX1 [Benincasa hispida])

HSP 1 Score: 517.7 bits (1332), Expect = 6.3e-143
Identity = 250/284 (88.03%), Postives = 264/284 (92.96%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELL+WP+KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 6   SLAPNAELLEWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 65

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYG+SSYDIGTGFGH AIATQDVYKLVEDIRAKGGSI +EP  V 
Sbjct: 66  GFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVK 125

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 126 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 185

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AISTDDVYKS EVV  V +
Sbjct: 186 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAISTDDVYKSAEVVNLVNQ 245

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+PGPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 246 ELGGKITRQPGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 289

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BLAST of MS010768 vs. NCBI nr
Match: XP_022142112.1 (putative lactoylglutathione lyase [Momordica charantia] >XP_022142113.1 putative lactoylglutathione lyase [Momordica charantia])

HSP 1 Score: 517.3 bits (1331), Expect = 8.3e-143
Identity = 251/282 (89.01%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 3   APNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGF 62
           APNAELL+W +KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLR RDVPEEKYSNAFLGF
Sbjct: 5   APNAELLEWAKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRHRDVPEEKYSNAFLGF 64

Query: 63  GPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLGG 122
           GPE SNFVVELTYNYGV SYDIGTGFGH AIATQDVYKLVEDIRAKGGSI +EP  V GG
Sbjct: 65  GPEESNFVVELTYNYGVCSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKGG 124

Query: 123 STVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMPE 182
           S+V+AF KDPDGY+FEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD PE
Sbjct: 125 SSVIAFAKDPDGYVFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRPE 184

Query: 183 YKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEEL 242
           YKYT+A++GYA ELE T LEL YNYGVTEYTKGTAYAQIAI TDDVYKS EVVKQV EEL
Sbjct: 185 YKYTIAMLGYADELETTVLELTYNYGVTEYTKGTAYAQIAIGTDDVYKSAEVVKQVHEEL 244

Query: 243 GGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           GGKITR+PGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE
Sbjct: 245 GGKITRQPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 286

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BLAST of MS010768 vs. ExPASy Swiss-Prot
Match: O65398 (Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 9.5e-134
Identity = 226/283 (79.86%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +E
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

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BLAST of MS010768 vs. ExPASy Swiss-Prot
Match: Q39366 (Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 PE=2 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 2.0e-131
Identity = 224/283 (79.15%), Postives = 253/283 (89.40%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A NA+L++WP+KDKRRFLHVVYRVGDLDR+I+FYTECFGMK+LRKRDVPEEKYSNAFLG
Sbjct: 1   MAENADLVEWPKKDKRRFLHVVYRVGDLDRTIQFYTECFGMKVLRKRDVPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV K+VE +RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKMVEAVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEPLCQV+LRVGDL+R++KF EKALGMRLL++++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPLCQVMLRVGDLDRAVKFMEKALGMRLLRRIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EY  T+ +MGYA E E+  LEL YNYGVTEYTKG AYAQIAI TDDVYKS EVVK V +E
Sbjct: 181 EYN-TIGMMGYAEEYESIVLELTYNYGVTEYTKGNAYAQIAIGTDDVYKSAEVVKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWK VLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKQVLVDNEDFLKELE 282

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BLAST of MS010768 vs. ExPASy Swiss-Prot
Match: Q948T6 (Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=1 SV=2)

HSP 1 Score: 439.9 bits (1130), Expect = 2.2e-122
Identity = 208/278 (74.82%), Postives = 240/278 (86.33%), Query Frame = 0


Query: 8   LLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEVS 67
           +L+WP+KDK+R LH VYRVGDLDR+IK YTECFGMKLLRKRDVPEEKY+NAFLGFGPE +
Sbjct: 14  VLEWPKKDKKRLLHAVYRVGDLDRTIKCYTECFGMKLLRKRDVPEEKYTNAFLGFGPEDT 73

Query: 68  NFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAK-GGSIIKEP-MVLGGSTVL 127
           NF +ELTYNYGV  YDIG GFGH AIAT+DVYKL E I++     I +EP  V GGSTV+
Sbjct: 74  NFALELTYNYGVDKYDIGAGFGHFAIATEDVYKLAEKIKSSCCCKITREPGPVKGGSTVI 133

Query: 128 AFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMPEYKYT 187
           AF +DPDGY+FEL QRGPTPEPLCQV+LRVGDL+RSIKFYEKALGM+LL+K D+P+YKYT
Sbjct: 134 AFAQDPDGYMFELIQRGPTPEPLCQVMLRVGDLDRSIKFYEKALGMKLLRKKDVPDYKYT 193

Query: 188 VAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEELGGKI 247
           +A++GYA E + T +EL YNYGVTEYTKG AYAQ+AI T+DVYKS E V+ VT+ELGGKI
Sbjct: 194 IAMLGYADEDKTTVIELTYNYGVTEYTKGNAYAQVAIGTEDVYKSAEAVELVTKELGGKI 253

Query: 248 TREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
            R+PGPLPGL TKI SFLDPDGWK VLVDN DFLKEL+
Sbjct: 254 LRQPGPLPGLNTKIASFLDPDGWKVVLVDNADFLKELQ 291

[Close]

BLAST of MS010768 vs. ExPASy Swiss-Prot
Match: Q8W593 (Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g67280 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.6e-115
Identity = 197/278 (70.86%), Postives = 227/278 (81.65%), Query Frame = 0


Query: 7   ELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEV 66
           +LL W + DKRR LHVVYRVGD+DR+IKFYTEC GMKLLRKRD+PEEKY+NAFLG+GPE 
Sbjct: 77  DLLTWVKNDKRRMLHVVYRVGDMDRTIKFYTECLGMKLLRKRDIPEEKYTNAFLGYGPED 136

Query: 67  SNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLGGSTVL 126
           S+FV+ELTYNYGV  YDIG GFGH  IA  DV K VE ++AKGG + +EP  V GG TV+
Sbjct: 137 SHFVIELTYNYGVDKYDIGAGFGHFGIAVDDVAKTVELVKAKGGKVSREPGPVKGGKTVI 196

Query: 127 AFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMPEYKYT 186
           AF++DPDGY FEL +RGPTPEPLCQV+LRVGDL+R+IKFYEKA GM LL+  D PEYKYT
Sbjct: 197 AFIEDPDGYKFELLERGPTPEPLCQVMLRVGDLDRAIKFYEKAFGMELLRTRDNPEYKYT 256

Query: 187 VAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEELGGKI 246
           +A+MGY  E +   LEL YNYGVTEY KG AYAQIAI TDDVYK+ E +K      GGKI
Sbjct: 257 IAMMGYGPEDKFPVLELTYNYGVTEYDKGNAYAQIAIGTDDVYKTAEAIKL----FGGKI 316

Query: 247 TREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           TREPGPLPG+ TKI + LDPDGWK+V VDN+DFLKELE
Sbjct: 317 TREPGPLPGISTKITACLDPDGWKSVFVDNIDFLKELE 350

[Close]

BLAST of MS010768 vs. ExPASy Swiss-Prot
Match: Q9KT93 (Probable lactoylglutathione lyase OS=Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) OX=243277 GN=gloA PE=3 SV=2)

HSP 1 Score: 143.7 bits (361), Expect = 3.3e-33
Identity = 66/124 (53.23%), Postives = 88/124 (70.97%), Query Frame = 0


Query: 18  RFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGFGPEVSNFVVELTYNY 77
           R LH + RVGDLD+SI+FYT+  GM LLRK +  E KY+ AFLG+G E    V+ELTYN+
Sbjct: 5   RILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNW 64

Query: 78  GVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLGGSTVLAFLKDPDGYIF 137
           GV+ Y+ G  +GH+AI   D+Y   + I+A GG + +EP  V GG+T +AF+KDPDGY+ 
Sbjct: 65  GVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMI 124

Query: 138 ELTQ 141
           EL Q
Sbjct: 125 ELIQ 128

[Close]

BLAST of MS010768 vs. ExPASy TrEMBL
Match: A0A6J1CME7 (Glyoxalase I OS=Momordica charantia OX=3673 GN=LOC111012317 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 7.3e-161
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 6   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 65

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG 120
           GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG
Sbjct: 66  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEPMVLG 125

Query: 121 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 180
           GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP
Sbjct: 126 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 185

Query: 181 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 240
           EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE
Sbjct: 186 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 245

Query: 241 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE
Sbjct: 246 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 288

[Close]

BLAST of MS010768 vs. ExPASy TrEMBL
Match: A0A1S3B032 (Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103484618 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 251/284 (88.38%), Postives = 263/284 (92.61%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWP+KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 7   SLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 66

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYGVSSYDIGTGFGH AIATQDVYKLVEDIRAKGG I +EP  V 
Sbjct: 67  GFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITREPGPVK 126

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 127 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 186

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AI TDDVYKS EVV QV +
Sbjct: 187 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVVNQVNQ 246

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+PGPLPG+GTKIV FLDPDGWKTVLVDN DFLKELE
Sbjct: 247 ELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELE 290

[Close]

BLAST of MS010768 vs. ExPASy TrEMBL
Match: A0A5D3CNL5 (Lactoylglutathione lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006420 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.1e-143
Identity = 251/284 (88.38%), Postives = 263/284 (92.61%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNAELLDWP+KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 7   SLAPNAELLDWPKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 66

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYGVSSYDIGTGFGH AIATQDVYKLVEDIRAKGG I +EP  V 
Sbjct: 67  GFGPEDSNFVVELTYNYGVSSYDIGTGFGHFAIATQDVYKLVEDIRAKGGIITREPGPVK 126

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 127 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 186

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AI TDDVYKS EVV QV +
Sbjct: 187 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVVNQVNQ 246

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+PGPLPG+GTKIV FLDPDGWKTVLVDN DFLKELE
Sbjct: 247 ELGGKITRQPGPLPGIGTKIVGFLDPDGWKTVLVDNADFLKELE 290

[Close]

BLAST of MS010768 vs. ExPASy TrEMBL
Match: A0A6J1CMF4 (Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111012318 PE=3 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.0e-143
Identity = 251/282 (89.01%), Postives = 263/282 (93.26%), Query Frame = 0


Query: 3   APNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLGF 62
           APNAELL+W +KDKRRFLHVVYRVGDLDR+IKFYTECFGMKLLR RDVPEEKYSNAFLGF
Sbjct: 5   APNAELLEWAKKDKRRFLHVVYRVGDLDRTIKFYTECFGMKLLRHRDVPEEKYSNAFLGF 64

Query: 63  GPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLGG 122
           GPE SNFVVELTYNYGV SYDIGTGFGH AIATQDVYKLVEDIRAKGGSI +EP  V GG
Sbjct: 65  GPEESNFVVELTYNYGVCSYDIGTGFGHFAIATQDVYKLVEDIRAKGGSITREPGPVKGG 124

Query: 123 STVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMPE 182
           S+V+AF KDPDGY+FEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD PE
Sbjct: 125 SSVIAFAKDPDGYVFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDRPE 184

Query: 183 YKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEEL 242
           YKYT+A++GYA ELE T LEL YNYGVTEYTKGTAYAQIAI TDDVYKS EVVKQV EEL
Sbjct: 185 YKYTIAMLGYADELETTVLELTYNYGVTEYTKGTAYAQIAIGTDDVYKSAEVVKQVHEEL 244

Query: 243 GGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           GGKITR+PGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE
Sbjct: 245 GGKITRQPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 286

[Close]

BLAST of MS010768 vs. ExPASy TrEMBL
Match: A0A6J1GDS2 (Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111453257 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 3.8e-141
Identity = 245/284 (86.27%), Postives = 263/284 (92.61%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           SLAPNA+LL+WP+KD+RRFLHVVYRVGDLDR+IKFYTECFGMKLLRKRDVPEEKYSNAFL
Sbjct: 6   SLAPNADLLEWPKKDRRRFLHVVYRVGDLDRTIKFYTECFGMKLLRKRDVPEEKYSNAFL 65

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYG+SSYDIGTGFGH AIA+QDVYKLVEDIRAKGGSI +EP  V 
Sbjct: 66  GFGPEDSNFVVELTYNYGISSYDIGTGFGHFAIASQDVYKLVEDIRAKGGSITREPGPVK 125

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GGS+V+AF KDPDGYIFEL QRGPTPEPLCQV+LRVGDLERSIKFYEKALGMRLLKKVD 
Sbjct: 126 GGSSVIAFAKDPDGYIFELIQRGPTPEPLCQVMLRVGDLERSIKFYEKALGMRLLKKVDR 185

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+A+MGYA ELE T LEL YNYGVTEYTKG AYAQ+AI TDDVYKS EV+K V +
Sbjct: 186 PEYKYTLAMMGYADELETTVLELTYNYGVTEYTKGNAYAQVAIGTDDVYKSAEVIKLVNQ 245

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITR+ GPLPG+GTKIVSFLDPDGWKTVLVDN DF KELE
Sbjct: 246 ELGGKITRQAGPLPGIGTKIVSFLDPDGWKTVLVDNADFQKELE 289

[Close]

BLAST of MS010768 vs. TAIR 10
Match: AT1G11840.6 (glyoxalase I homolog )

HSP 1 Score: 478.0 bits (1229), Expect = 5.2e-135
Identity = 226/284 (79.58%), Postives = 255/284 (89.79%), Query Frame = 0


Query: 1   SLAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFL 60
           ++A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFL
Sbjct: 39  NMAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFL 98

Query: 61  GFGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVL 120
           GFGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V 
Sbjct: 99  GFGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVK 158

Query: 121 GGSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDM 180
           GG +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ 
Sbjct: 159 GGGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIER 218

Query: 181 PEYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTE 240
           PEYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +
Sbjct: 219 PEYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQ 278

Query: 241 ELGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           ELGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 279 ELGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 322

[Close]

BLAST of MS010768 vs. TAIR 10
Match: AT1G11840.2 (glyoxalase I homolog )

HSP 1 Score: 477.6 bits (1228), Expect = 6.8e-135
Identity = 226/283 (79.86%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +E
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MS010768 vs. TAIR 10
Match: AT1G11840.3 (glyoxalase I homolog )

HSP 1 Score: 477.6 bits (1228), Expect = 6.8e-135
Identity = 226/283 (79.86%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +E
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MS010768 vs. TAIR 10
Match: AT1G11840.1 (glyoxalase I homolog )

HSP 1 Score: 477.6 bits (1228), Expect = 6.8e-135
Identity = 226/283 (79.86%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +E
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

[Close]

BLAST of MS010768 vs. TAIR 10
Match: AT1G11840.4 (glyoxalase I homolog )

HSP 1 Score: 477.6 bits (1228), Expect = 6.8e-135
Identity = 226/283 (79.86%), Postives = 254/283 (89.75%), Query Frame = 0


Query: 2   LAPNAELLDWPRKDKRRFLHVVYRVGDLDRSIKFYTECFGMKLLRKRDVPEEKYSNAFLG 61
           +A  ++LL+WP+KD RRFLHVVYRVGDLDR+I+FYTE FGMKLLRKRD+PEEKYSNAFLG
Sbjct: 1   MAEASDLLEWPKKDNRRFLHVVYRVGDLDRTIEFYTEVFGMKLLRKRDIPEEKYSNAFLG 60

Query: 62  FGPEVSNFVVELTYNYGVSSYDIGTGFGHLAIATQDVYKLVEDIRAKGGSIIKEP-MVLG 121
           FGPE SNFVVELTYNYGVSSYDIGTGFGH AI+TQDV KLVE++RAKGG++ +EP  V G
Sbjct: 61  FGPETSNFVVELTYNYGVSSYDIGTGFGHFAISTQDVSKLVENVRAKGGNVTREPGPVKG 120

Query: 122 GSTVLAFLKDPDGYIFELTQRGPTPEPLCQVLLRVGDLERSIKFYEKALGMRLLKKVDMP 181
           G +V+AF+KDPDGY FEL QRGPTPEP CQV+LRVGDL+R+IKFYEKALGMRLL+K++ P
Sbjct: 121 GGSVIAFVKDPDGYTFELIQRGPTPEPFCQVMLRVGDLDRAIKFYEKALGMRLLRKIERP 180

Query: 182 EYKYTVAVMGYAGELEATALELAYNYGVTEYTKGTAYAQIAISTDDVYKSGEVVKQVTEE 241
           EYKYT+ +MGYA E E+  LEL YNY VTEYTKG AYAQIAI TDDVYKSGEV+K V +E
Sbjct: 181 EYKYTIGMMGYAEEYESIVLELTYNYDVTEYTKGNAYAQIAIGTDDVYKSGEVIKIVNQE 240

Query: 242 LGGKITREPGPLPGLGTKIVSFLDPDGWKTVLVDNVDFLKELE 284
           LGGKITRE GPLPGLGTKIVSFLDPDGWKTVLVDN DFLKELE
Sbjct: 241 LGGKITREAGPLPGLGTKIVSFLDPDGWKTVLVDNKDFLKELE 283

The following BLAST results are available for this feature:

BLAST of MS010768 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142108.1	1.5e-160	100.00	putative lactoylglutathione lyase isoform X1 [Momordica charantia]	[more]
TYK13040.1	2.2e-143	88.38	putative lactoylglutathione lyase [Cucumis melo var. makuwa]	[more]
XP_008440010.1	2.2e-143	88.38	PREDICTED: putative lactoylglutathione lyase [Cucumis melo] >XP_008440011.1 PRED...	[more]
XP_038882184.1	6.3e-143	88.03	lactoylglutathione lyase GLX1 [Benincasa hispida] >XP_038882185.1 lactoylglutath...	[more]
XP_022142112.1	8.3e-143	89.01	putative lactoylglutathione lyase [Momordica charantia] >XP_022142113.1 putative...	[more]

BLAST of MS010768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65398	9.5e-134	79.86	Lactoylglutathione lyase GLX1 OS=Arabidopsis thaliana OX=3702 GN=GLX1 PE=1 SV=1	[more]
Q39366	2.0e-131	79.15	Putative lactoylglutathione lyase OS=Brassica oleracea var. gemmifera OX=178616 ...	[more]
Q948T6	2.2e-122	74.82	Lactoylglutathione lyase OS=Oryza sativa subsp. japonica OX=39947 GN=GLYI-11 PE=...	[more]
Q8W593	2.6e-115	70.86	Probable lactoylglutathione lyase, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
Q9KT93	3.3e-33	53.23	Probable lactoylglutathione lyase OS=Vibrio cholerae serotype O1 (strain ATCC 39...	[more]

BLAST of MS010768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CME7	7.3e-161	100.00	Glyoxalase I OS=Momordica charantia OX=3673 GN=LOC111012317 PE=3 SV=1	[more]
A0A1S3B032	1.1e-143	88.38	Lactoylglutathione lyase OS=Cucumis melo OX=3656 GN=LOC103484618 PE=3 SV=1	[more]
A0A5D3CNL5	1.1e-143	88.38	Lactoylglutathione lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A6J1CMF4	4.0e-143	89.01	Lactoylglutathione lyase OS=Momordica charantia OX=3673 GN=LOC111012318 PE=3 SV=...	[more]
A0A6J1GDS2	3.8e-141	86.27	Lactoylglutathione lyase OS=Cucurbita moschata OX=3662 GN=LOC111453257 PE=3 SV=1	[more]

BLAST of MS010768 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11840.6	5.2e-135	79.58	glyoxalase I homolog	[more]
AT1G11840.2	6.8e-135	79.86	glyoxalase I homolog	[more]
AT1G11840.3	6.8e-135	79.86	glyoxalase I homolog	[more]
AT1G11840.1	6.8e-135	79.86	glyoxalase I homolog	[more]
AT1G11840.4	6.8e-135	79.86	glyoxalase I homolog	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004361	Glyoxalase I	TIGRFAM	TIGR00068	TIGR00068	coord: 5..150 e-value: 5.0E-60 score: 199.3 coord: 148..276 e-value: 3.5E-40 score: 134.9
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 144..283 e-value: 7.9E-38 score: 131.8
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	GENE3D	3.10.180.10		coord: 2..142 e-value: 1.9E-50 score: 172.8
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 17..146
IPR029068	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	SUPERFAMILY	54593	Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase	coord: 148..275
IPR004360	Glyoxalase/fosfomycin resistance/dioxygenase domain	PFAM	PF00903	Glyoxalase	coord: 19..138 e-value: 9.9E-20 score: 71.0 coord: 151..269 e-value: 9.0E-14 score: 51.8
None	No IPR available	PANTHER	PTHR46036:SF8	LACTOYLGLUTATHIONE LYASE-LIKE PROTEIN	coord: 8..283
None	No IPR available	PANTHER	PTHR46036	LACTOYLGLUTATHIONE LYASE	coord: 8..283
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00934	GLYOXALASE_I_1	coord: 150..171
IPR018146	Glyoxalase I, conserved site	PROSITE	PS00934	GLYOXALASE_I_1	coord: 21..42
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 147..275 score: 20.33046
IPR037523	Vicinal oxygen chelate (VOC) domain	PROSITE	PS51819	VOC	coord: 18..141 score: 28.926065

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010768.1	MS010768.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004462	lactoylglutathione lyase activity
molecular_function	GO:0046872	metal ion binding