MS010745 (gene) Bitter gourd (TR) v1

BLAST of MS010745 vs. NCBI nr
Match: XP_022132784.1 (AAA ATPase forming ring-shaped complexes isoform X1 [Momordica charantia])

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 575/588 (97.79%), Postives = 578/588 (98.30%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP
Sbjct: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVTPPQPNDLTNHADMEEIAKRERRRIEQLIRNKGLKY 120
           AIIVSTFGVGAMEIA+AEAEESAVTPPQPNDLT HADMEEIAKRERRRIEQLIRNKGLKY
Sbjct: 61  AIIVSTFGVGAMEIAYAEAEESAVTPPQPNDLTTHADMEEIAKRERRRIEQLIRNKGLKY 120

Query: 121 GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV 180
           GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV
Sbjct: 121 GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV 180

Query: 181 AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE 240
           AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE
Sbjct: 181 AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE 240

Query: 241 LDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVNSS 300
           LDSLVSVLQLAGGRLGESKSFESKSREGSTQM SSEKSISSLEAMGVK+YGLDEPRVNSS
Sbjct: 241 LDSLVSVLQLAGGRLGESKSFESKSREGSTQMPSSEKSISSLEAMGVKVYGLDEPRVNSS 300

Query: 301 NNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGT 360
           NNEISWDNIAGYDQQK EIEDTILMTLHNPELFDDIA GTRHKFESNRPRAVLFEGPPGT
Sbjct: 301 NNEISWDNIAGYDQQKGEIEDTILMTLHNPELFDDIARGTRHKFESNRPRAVLFEGPPGT 360

Query: 361 GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS 420
           GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS
Sbjct: 361 GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS 420

Query: 421 FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG 480
           FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG
Sbjct: 421 FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG 480

Query: 481 LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIRGQ 540
           LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAER   S   +IIRGQ
Sbjct: 481 LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAER---SWASKIIRGQ 540

Query: 541 VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA
Sbjct: 541 VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 585

BLAST of MS010745 vs. NCBI nr
Match: XP_038882484.1 (26S proteasome subunit RPT4 isoform X1 [Benincasa hispida])

HSP 1 Score: 971.1 bits (2509), Expect = 4.4e-279
Identity = 506/589 (85.91%), Postives = 539/589 (91.51%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RTARAWRI+ SFKLKD SS SRSLY R F+ Q  +L+P S+ NGRE  FLYP
Sbjct: 1   MSVLERIARTARAWRILTSFKLKDSSSASRSLYLRHFRHQPEHLKPGSLSNGREPCFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVTPPQPNDLTNHADMEEIAKRERRR-IEQLIRNKGLK 120
           AII   FGVGAMEIA+AEAEES+VTPP P DL+ HADMEEIAK+ER+R IEQL RNKG K
Sbjct: 61  AIIAGMFGVGAMEIAYAEAEESSVTPPPPKDLSTHADMEEIAKKERQRIIEQLTRNKGTK 120

Query: 121 YGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSP 180
           YGACPRF VGVKG KV+IKFQVPSSCEVSHL+AN+VSNLGLKVEE AGGSDMLLRAWDSP
Sbjct: 121 YGACPRFMVGVKGPKVSIKFQVPSSCEVSHLLANLVSNLGLKVEESAGGSDMLLRAWDSP 180

Query: 181 VAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTK 240
           VAWQLTLT PKTQRE+G N+G S+EMDAEDGDLSVLIFHSL+TSDKTEIEFIKQGSLSTK
Sbjct: 181 VAWQLTLTRPKTQRESGENKGNSLEMDAEDGDLSVLIFHSLVTSDKTEIEFIKQGSLSTK 240

Query: 241 ELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVNS 300
           ELDSLVSVLQLAGGRLGES+SFE KSREGSTQM SSE SISSLEAMGVK+YGLDEP   S
Sbjct: 241 ELDSLVSVLQLAGGRLGESRSFERKSREGSTQMPSSENSISSLEAMGVKVYGLDEPGEYS 300

Query: 301 SNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPG 360
           S +EISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTR KFESNRPRAVLFEGPPG
Sbjct: 301 SKSEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRRKFESNRPRAVLFEGPPG 360

Query: 361 TGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVD 420
           TGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLGKVFSLANELS GAIIFLDEVD
Sbjct: 361 TGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSTGAIIFLDEVD 420

Query: 421 SFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITF 480
           SFAI+RDSE+HEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD MITF
Sbjct: 421 SFAISRDSEIHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDLMITF 480

Query: 481 GLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIRG 540
           GLPDERNR+EIA+QYAKQLTKPELNEFAR TEGMSGRDIRDICQQAER   S   +IIRG
Sbjct: 481 GLPDERNREEIAAQYAKQLTKPELNEFARNTEGMSGRDIRDICQQAER---SWASKIIRG 540

Query: 541 QVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           +VSKTGEHGILPPL+EYI CAMSRRKALQT+D+++IKD N RTKKTQLA
Sbjct: 541 KVSKTGEHGILPPLEEYIECAMSRRKALQTIDNHEIKDPNFRTKKTQLA 586

BLAST of MS010745 vs. NCBI nr
Match: XP_022977688.1 (spermatogenesis-associated protein 5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 966.1 bits (2496), Expect = 1.4e-277
Identity = 503/590 (85.25%), Postives = 539/590 (91.36%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RT +AWRII SFKLKD SS S SLY R F+ Q  +L+P  + NGRE   LYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCLLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVT-PPQPNDLTNHADMEEIAKRERRRI-EQLIRNKGL 120
           AI+ S FGVGAMEIA+AEAEESAVT PP P DL+ HAD+EEIAK+ER+RI EQLIRNKG+
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 121 KYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDS 180
           KYGACPRFTVGVKGQKV+IKFQVPS+CEVSHLVAN+VSNLGLKVEERAGGSDMLLRAW S
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSNCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 181 PVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLST 240
           PVAWQL+LT PKTQREAGGNEG S+E+DA DGDLSVLIFHSL++SDKTEIEF+KQGSLST
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDGDLSVLIFHSLVSSDKTEIEFLKQGSLST 240

Query: 241 KELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVN 300
           KELDSLVSVLQLAGGRLGES+SFE KSREGSTQ+   EKSISSLE+MGVK+YGLDEP VN
Sbjct: 241 KELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESMGVKVYGLDEPHVN 300

Query: 301 SSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPP 360
           SS NEISWDNIAGYDQQK EIEDTIL+TLHNPELFD+IAHGTR KFESNRPRAVLFEGPP
Sbjct: 301 SSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP 360

Query: 361 GTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEV 420
           GTGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLG+VFSLANELS GAIIFLDEV
Sbjct: 361 GTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEV 420

Query: 421 DSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMIT 480
           DSFAI RDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD MIT
Sbjct: 421 DSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDLMIT 480

Query: 481 FGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIR 540
           FGLPDERNRQEIA+QYAKQLTKPELNEFA+ TEGMSGRDIRDICQQAER   S   +I+R
Sbjct: 481 FGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQAER---SWASKILR 540

Query: 541 GQVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           G+VSKTGEHGILPPLQEYI CAMSRRKALQTVDD+KIKDSN RTKKTQ A
Sbjct: 541 GKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 587

BLAST of MS010745 vs. NCBI nr
Match: KAG6603800.1 (hypothetical protein SDJN03_04409, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 958.0 bits (2475), Expect = 3.8e-275
Identity = 501/594 (84.34%), Postives = 536/594 (90.24%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RT +AWRII SFKLKD SS S SLY R F+ Q  +L+P  + NGRE  FLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVT-----PPQPNDLTNHADMEEIAKRERRRI-EQLIR 120
           AI+ S FGVGAMEIA+AEAEESAVT     PP P DL+ HAD+EEIAK+ER+RI EQLIR
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPKDLSTHADLEEIAKKERQRINEQLIR 120

Query: 121 NKGLKYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLR 180
           NKG+KYGACPRFTVGVKGQ V+IKFQVPSSCEVSHLVAN+VSNLGLKVEERAGGSDMLLR
Sbjct: 121 NKGMKYGACPRFTVGVKGQTVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 180

Query: 181 AWDSPVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQG 240
           AW SPVAWQL+LT PKTQREAGGNEG S+E+DA D DLSVLIFHSL++SDKTEIEF+KQG
Sbjct: 181 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTEIEFLKQG 240

Query: 241 SLSTKELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDE 300
           SLSTKELDSLVSVLQLAGGRLGES+SFE KSREGSTQ+   EKSISSLE+MGVK+YGLDE
Sbjct: 241 SLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESMGVKVYGLDE 300

Query: 301 PRVNSSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLF 360
             VNSS NEISWDNIAGYDQQK EIEDTIL+TLHNPELFD+IAHGTR KFESNRPRAVLF
Sbjct: 301 QHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLF 360

Query: 361 EGPPGTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIF 420
           EGPPGTGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLG+VFSLANELS GAIIF
Sbjct: 361 EGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIF 420

Query: 421 LDEVDSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD 480
           LDEVDSFAI RDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD
Sbjct: 421 LDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD 480

Query: 481 SMITFGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFG 540
            MITFGLPDERNRQEIA+QYAKQLTKPELNEFA+ TEGMSGRDIRDICQQAER   S   
Sbjct: 481 LMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQAER---SWAS 540

Query: 541 QIIRGQVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           +I+RG+VSKTGEHGILPPLQEYI CAMSRRKALQTVDD+K KDSN RTKKTQ A
Sbjct: 541 KILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKFKDSNIRTKKTQFA 591

BLAST of MS010745 vs. NCBI nr
Match: KAG7033971.1 (pan, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 956.8 bits (2472), Expect = 8.5e-275
Identity = 501/594 (84.34%), Postives = 536/594 (90.24%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RT +AWRII SFKLKD SS S SLY R F+ Q  +L+P  + NGRE  FLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVT-----PPQPNDLTNHADMEEIAKRERRRI-EQLIR 120
           AI+ S FGVGAMEIA+AEAEESAVT     PP P DL+ HAD+EEIAK+ER+RI EQLIR
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPKDLSTHADLEEIAKKERQRINEQLIR 120

Query: 121 NKGLKYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLR 180
           NKG+KYGACPRFTVGVKGQKV+IKFQVPSSCEVSHLVAN+VSNLGLKVEERAGGSDMLLR
Sbjct: 121 NKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDMLLR 180

Query: 181 AWDSPVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQG 240
           AW SPVAWQL+LT PKTQREAGGNEG S+E+DA D DLSVLIFHSL++SDKTEIEF+KQG
Sbjct: 181 AWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTEIEFLKQG 240

Query: 241 SLSTKELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDE 300
           SLSTKELDSLVSVLQLAGGRLGES+SFE KSREGSTQ+   EKSISSLE+MGVK+YGLDE
Sbjct: 241 SLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESMGVKVYGLDE 300

Query: 301 PRVNSSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLF 360
             VNSS NEISWDNIAGYDQQK EIEDTIL+TLHNPELFD+IAHGTR KFESNRPRAVLF
Sbjct: 301 QHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLF 360

Query: 361 EGPPGTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIF 420
           EGPPGTGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLG+VFSLANELS GAIIF
Sbjct: 361 EGPPGTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIF 420

Query: 421 LDEVDSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD 480
           LDEVDSFAI RDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD
Sbjct: 421 LDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD 480

Query: 481 SMITFGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFG 540
            MITFGLPDERNRQEIA+QYAKQLTKPELNEFA+ TEGMSGRDIRDICQQAER   S   
Sbjct: 481 LMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQAER---SWAS 540

Query: 541 QIIRGQVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           +I+RG+VSKTGEHGILPPLQEYI CAMSRRKALQTVDD+KIKDSN   KKTQ A
Sbjct: 541 KILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIGMKKTQFA 591

BLAST of MS010745 vs. ExPASy Swiss-Prot
Match: Q8TX03 (Proteasome-activating nucleotidase OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=pan PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 1.0e-36
Identity = 95/234 (40.60%), Postives = 134/234 (57.26%), Query Frame = 0

Query: 303 EISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGK 362
           ++S+D+I G D+Q REI + +   L  PELF+        K     P+ VL  GPPGTGK
Sbjct: 175 DVSYDDIGGLDEQIREIREVVEKPLKEPELFE--------KVGVEPPKGVLLYGPPGTGK 234

Query: 363 TSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFA 422
           T  A+ +AN AD   + +    ++ K+ GE  RL+ ++F LA E +  +IIF+DE+D+  
Sbjct: 235 TLLAKAVANHADATFIRLAAPELVQKFIGEGARLVRELFELAREKAP-SIIFIDEIDAIG 294

Query: 423 ITRDSEMHEAT------RRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFD 482
             R   M +AT      +R L+ LL ++DGF+    + VIAATNRK  LDPAL+   RFD
Sbjct: 295 ARR---MRDATSGDREVQRTLTQLLAEMDGFDPLDDIKVIAATNRKDILDPALLRPGRFD 354

Query: 483 SMITFGLPDERNRQEIASQYAKQLTKPE---LNEFARITEGMSGRDIRDICQQA 526
             I   LPDE  R EI   + + +   E   L + A+ITEG SG DI+ IC +A
Sbjct: 355 RHIKIPLPDEEGRYEIFKIHTRDMNLAEDVDLQKLAKITEGASGADIKAICTEA 396

BLAST of MS010745 vs. ExPASy Swiss-Prot
Match: O28972 (Cell division cycle protein 48 homolog AF_1297 OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1297 PE=3 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 8.6e-36
Identity = 88/228 (38.60%), Postives = 132/228 (57.89%), Query Frame = 0

Query: 303 EISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGK 362
           ++++++I G  ++ R + + I + L +PELF         +     P+ VL  GPPGTGK
Sbjct: 178 DVTYEDIGGLKRELRLVREMIELPLKHPELF--------QRLGIEPPKGVLLYGPPGTGK 237

Query: 363 TSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFA 422
           T  A+ +AN+ D   + +    IMSKYYGESE+ L ++F  A E +  +IIF+DE+DS A
Sbjct: 238 TLIAKAVANEVDAHFIPISGPEIMSKYYGESEQRLREIFEEAKE-NAPSIIFIDEIDSIA 297

Query: 423 ITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPAL--ISRFDSMITFG 482
             R+    E  RRV++ LL  +DG E    V+VIAATNR   +DPAL    RFD  I  G
Sbjct: 298 PKREEVTGEVERRVVAQLLALMDGLEARGDVIVIAATNRPDAIDPALRRPGRFDREIEIG 357

Query: 483 LPDERNRQEIASQYAKQLTKPE---LNEFARITEGMSGRDIRDICQQA 526
           +PD+  R+EI   + +++   E   L E A +T G  G D+  +C++A
Sbjct: 358 VPDKEGRKEILEIHTRKMPLAEDVDLEELAELTNGFVGADLEALCKEA 396

BLAST of MS010745 vs. ExPASy Swiss-Prot
Match: O17071 (Probable 26S proteasome regulatory subunit 10B OS=Caenorhabditis elegans OX=6239 GN=rpt-4 PE=1 SV=2)

HSP 1 Score: 149.4 bits (376), Expect = 1.2e-34
Identity = 111/332 (33.43%), Postives = 166/332 (50.00%), Query Frame = 0

Query: 225 KTEIEFIKQGSLSTKELDSLVSVLQLAGGRLGE--SKSFESKSREGSTQMSSSEKSISSL 284
           K E E  KQ   S  ++ SL SV Q+ G  L +   + F  K+  G   +    +SI+  
Sbjct: 48  KKESEMTKQFDKSENDIKSLQSVGQIVGEVLKQLSEEKFIVKATNGPRYVVGCRRSINKE 107

Query: 285 EAMGVKIYGLD--------------EPRVNSSNNE----ISWDNIAGYDQQKREIEDTIL 344
           E        LD              +P V   ++E    IS+ ++ G  +Q RE+ + + 
Sbjct: 108 ELKQGTRVSLDMTTLTIMRQLPREVDPLVYKMSHEDPGNISYSDVGGLAEQIRELREVVE 167

Query: 345 MTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYVPLEV 404
           + L NPELF  +            P+  L  GPPGTGKT  AR +A+Q D   + V    
Sbjct: 168 LPLINPELFKRVG--------ITPPKGCLLFGPPGTGKTLLARAVASQLDCNFLKVVSSA 227

Query: 405 IMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAITRDSEMHEATR---RVLSVLL 464
           I+ KY GES R++ ++F+ A +     I+F+DE+D+    R SE   A R   R L  LL
Sbjct: 228 IVDKYIGESARMIREMFNYARD-HQPCIVFMDEIDAIGGRRFSEGTSADREIQRTLMELL 287

Query: 465 RQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFDSMITFGLPDERNRQEIASQYAKQLT 524
            Q+DGF+   KV VI ATNR   LDPAL+   R D  I  GLP+E++R EI   ++ ++T
Sbjct: 288 NQLDGFDSLGKVKVIMATNRPDTLDPALLRPGRLDRKIEIGLPNEQSRLEILKIHSNKIT 347

Query: 525 KP---ELNEFARITEGMSGRDIRDICQQAERF 529
           K    +     ++++G S  D+R++C +A  F
Sbjct: 348 KHGEIDFEAVVKLSDGFSAADLRNVCTEAGMF 370

BLAST of MS010745 vs. ExPASy Swiss-Prot
Match: O26824 (Proteasome-activating nucleotidase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=pan PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 2.1e-34
Identity = 88/234 (37.61%), Postives = 130/234 (55.56%), Query Frame = 0

Query: 303 EISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGK 362
           ++S++ I G ++Q RE+++T+ + L  PELF+        K     P+ VL  GPPGTGK
Sbjct: 147 DVSYEQIGGLEEQVREVKETVELPLKKPELFE--------KIGIEPPKGVLLYGPPGTGK 206

Query: 363 TSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFA 422
           T  A+ +A++ +   + +     + KY GE  RL+  VF LA E S  +IIF+DE+D+ A
Sbjct: 207 TLLAKAVAHETNATFIKIVASEFVRKYIGEGARLVRGVFELAKEKSP-SIIFIDEIDAVA 266

Query: 423 ITR---DSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFDSMI 482
             R    +      +R L  LL ++DGFE    V ++AATNR   LDPAL+   RFD  I
Sbjct: 267 AKRLKSSTSGDREVQRTLMQLLAELDGFESRGNVGIVAATNRPDILDPALLRPGRFDRFI 326

Query: 483 TFGLPDERNRQEIASQYAKQLTKPE---LNEFARITEGMSGRDIRDICQQAERF 529
              LP+E  R+EI   +   +   E   +   ARIT+G SG D++ IC +A  F
Sbjct: 327 EVPLPNEDGRREILKIHTSGMALAEEVDIELLARITDGASGADLKAICTEAGMF 371

BLAST of MS010745 vs. ExPASy Swiss-Prot
Match: Q60QD1 (Fidgetin-like protein 1 OS=Caenorhabditis briggsae OX=6238 GN=figl-1 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 6.1e-34
Identity = 83/264 (31.44%), Postives = 139/264 (52.65%), Query Frame = 0

Query: 268 GSTQMSSSEKSISSLEAMGVKIYGLDEPRVNSSNNEISWDNIAGYDQQKREIEDTILMTL 327
           G  +     +S  +L+     I  L E  + S NN+I W ++AG +  K+ +++ +++  
Sbjct: 277 GKDERMDGLRSEPTLKHFDENIISLIESEIMSVNNQIGWADVAGLEGAKKALKEIVVLPF 336

Query: 328 HNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYVPLEVIMS 387
             P++F  +            P+ VL  GPPGTGKT   R +A+QA      +    + S
Sbjct: 337 QRPDIFTGL---------RAPPKGVLLFGPPGTGKTMIGRCVASQAQATFFNISASSLTS 396

Query: 388 KYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAITRDSEMHEATRRVLSVLLRQIDGF 447
           K+ GE E+L+  +FS+A  L   ++IF+DE+DS    R    HE++RR+ +  L Q+DG 
Sbjct: 397 KWVGEGEKLVRALFSVA-RLKLPSVIFIDEIDSLLSARSESEHESSRRIKTEFLVQLDGV 456

Query: 448 E--QDRKVVVIAATNRKQDLDPALISRFDSMITFGLPDERNRQEIASQYAK----QLTKP 507
               D +++V+ ATNR Q+LD A   RF   +   LP+  +R +I     +    ++T  
Sbjct: 457 NTAPDERLLVLGATNRPQELDEAARRRFQKRLYIALPEPDSRTQIVENLLRGTRHEITDH 516

Query: 508 ELNEFARITEGMSGRDIRDICQQA 526
            L +  R+T+G SG D+R +C +A
Sbjct: 517 NLEKIRRLTDGYSGADMRQLCTEA 530

BLAST of MS010745 vs. ExPASy TrEMBL
Match: A0A6J1BU31 (AAA ATPase forming ring-shaped complexes isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005560 PE=3 SV=1)

HSP 1 Score: 1111.3 bits (2873), Expect = 0.0e+00
Identity = 575/588 (97.79%), Postives = 578/588 (98.30%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP
Sbjct: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVTPPQPNDLTNHADMEEIAKRERRRIEQLIRNKGLKY 120
           AIIVSTFGVGAMEIA+AEAEESAVTPPQPNDLT HADMEEIAKRERRRIEQLIRNKGLKY
Sbjct: 61  AIIVSTFGVGAMEIAYAEAEESAVTPPQPNDLTTHADMEEIAKRERRRIEQLIRNKGLKY 120

Query: 121 GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV 180
           GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV
Sbjct: 121 GACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPV 180

Query: 181 AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE 240
           AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE
Sbjct: 181 AWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKE 240

Query: 241 LDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVNSS 300
           LDSLVSVLQLAGGRLGESKSFESKSREGSTQM SSEKSISSLEAMGVK+YGLDEPRVNSS
Sbjct: 241 LDSLVSVLQLAGGRLGESKSFESKSREGSTQMPSSEKSISSLEAMGVKVYGLDEPRVNSS 300

Query: 301 NNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGT 360
           NNEISWDNIAGYDQQK EIEDTILMTLHNPELFDDIA GTRHKFESNRPRAVLFEGPPGT
Sbjct: 301 NNEISWDNIAGYDQQKGEIEDTILMTLHNPELFDDIARGTRHKFESNRPRAVLFEGPPGT 360

Query: 361 GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS 420
           GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS
Sbjct: 361 GKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDS 420

Query: 421 FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG 480
           FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG
Sbjct: 421 FAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFG 480

Query: 481 LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIRGQ 540
           LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAER   S   +IIRGQ
Sbjct: 481 LPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAER---SWASKIIRGQ 540

Query: 541 VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA
Sbjct: 541 VSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 585

BLAST of MS010745 vs. ExPASy TrEMBL
Match: A0A6J1IQS3 (spermatogenesis-associated protein 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477922 PE=3 SV=1)

HSP 1 Score: 966.1 bits (2496), Expect = 6.8e-278
Identity = 503/590 (85.25%), Postives = 539/590 (91.36%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RT +AWRII SFKLKD SS S SLY R F+ Q  +L+P  + NGRE   LYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCLLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVT-PPQPNDLTNHADMEEIAKRERRRI-EQLIRNKGL 120
           AI+ S FGVGAMEIA+AEAEESAVT PP P DL+ HAD+EEIAK+ER+RI EQLIRNKG+
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPKDLSTHADLEEIAKKERQRINEQLIRNKGM 120

Query: 121 KYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDS 180
           KYGACPRFTVGVKGQKV+IKFQVPS+CEVSHLVAN+VSNLGLKVEERAGGSDMLLRAW S
Sbjct: 121 KYGACPRFTVGVKGQKVSIKFQVPSNCEVSHLVANLVSNLGLKVEERAGGSDMLLRAWAS 180

Query: 181 PVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLST 240
           PVAWQL+LT PKTQREAGGNEG S+E+DA DGDLSVLIFHSL++SDKTEIEF+KQGSLST
Sbjct: 181 PVAWQLSLTRPKTQREAGGNEGNSLEIDAGDGDLSVLIFHSLVSSDKTEIEFLKQGSLST 240

Query: 241 KELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVN 300
           KELDSLVSVLQLAGGRLGES+SFE KSREGSTQ+   EKSISSLE+MGVK+YGLDEP VN
Sbjct: 241 KELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESMGVKVYGLDEPHVN 300

Query: 301 SSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPP 360
           SS NEISWDNIAGYDQQK EIEDTIL+TLHNPELFD+IAHGTR KFESNRPRAVLFEGPP
Sbjct: 301 SSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAVLFEGPP 360

Query: 361 GTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEV 420
           GTGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLG+VFSLANELS GAIIFLDEV
Sbjct: 361 GTGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGQVFSLANELSTGAIIFLDEV 420

Query: 421 DSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMIT 480
           DSFAI RDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFD MIT
Sbjct: 421 DSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDLMIT 480

Query: 481 FGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIR 540
           FGLPDERNRQEIA+QYAKQLTKPELNEFA+ TEGMSGRDIRDICQQAER   S   +I+R
Sbjct: 481 FGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQAER---SWASKILR 540

Query: 541 GQVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           G+VSKTGEHGILPPLQEYI CAMSRRKALQTVDD+KIKDSN RTKKTQ A
Sbjct: 541 GKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIRTKKTQFA 587

BLAST of MS010745 vs. ExPASy TrEMBL
Match: A0A6J1BTG0 (probable 26S proteasome regulatory subunit 10B isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005560 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 4.1e-275
Identity = 495/508 (97.44%), Postives = 498/508 (98.03%), Query Frame = 0

Query: 81  ESAVTPPQPNDLTNHADMEEIAKRERRRIEQLIRNKGLKYGACPRFTVGVKGQKVTIKFQ 140
           +SAVTPPQPNDLT HADMEEIAKRERRRIEQLIRNKGLKYGACPRFTVGVKGQKVTIKFQ
Sbjct: 9   QSAVTPPQPNDLTTHADMEEIAKRERRRIEQLIRNKGLKYGACPRFTVGVKGQKVTIKFQ 68

Query: 141 VPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPVAWQLTLTNPKTQREAGGNEG 200
           VPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPVAWQLTLTNPKTQREAGGNEG
Sbjct: 69  VPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSPVAWQLTLTNPKTQREAGGNEG 128

Query: 201 ISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKELDSLVSVLQLAGGRLGESKS 260
           ISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKELDSLVSVLQLAGGRLGESKS
Sbjct: 129 ISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTKELDSLVSVLQLAGGRLGESKS 188

Query: 261 FESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVNSSNNEISWDNIAGYDQQKREIE 320
           FESKSREGSTQM SSEKSISSLEAMGVK+YGLDEPRVNSSNNEISWDNIAGYDQQK EIE
Sbjct: 189 FESKSREGSTQMPSSEKSISSLEAMGVKVYGLDEPRVNSSNNEISWDNIAGYDQQKGEIE 248

Query: 321 DTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYV 380
           DTILMTLHNPELFDDIA GTRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYV
Sbjct: 249 DTILMTLHNPELFDDIARGTRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYV 308

Query: 381 PLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAITRDSEMHEATRRVLSVL 440
           PLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAITRDSEMHEATRRVLSVL
Sbjct: 309 PLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAITRDSEMHEATRRVLSVL 368

Query: 441 LRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFGLPDERNRQEIASQYAKQLTK 500
           LRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFGLPDERNRQEIASQYAKQLTK
Sbjct: 369 LRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITFGLPDERNRQEIASQYAKQLTK 428

Query: 501 PELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIRGQVSKTGEHGILPPLQEYIVCA 560
           PELNEFARITEGMSGRDIRDICQQAER   S   +IIRGQVSKTGEHGILPPLQEYIVCA
Sbjct: 429 PELNEFARITEGMSGRDIRDICQQAER---SWASKIIRGQVSKTGEHGILPPLQEYIVCA 488

Query: 561 MSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           MSRRKALQTVDDNKIKDSNSRTKKTQLA
Sbjct: 489 MSRRKALQTVDDNKIKDSNSRTKKTQLA 513

BLAST of MS010745 vs. ExPASy TrEMBL
Match: A0A6J1GDJ0 (nuclear valosin-containing protein-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453188 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.6e-274
Identity = 500/596 (83.89%), Postives = 536/596 (89.93%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++LERI RT +AWRII SFKLKD SS S SLY R F+ Q  +L+P  + NGRE  FLYP
Sbjct: 1   MSVLERIARTVKAWRIIKSFKLKDSSSVSPSLYLRHFRHQPEHLKPGFLSNGREPCFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVT-------PPQPNDLTNHADMEEIAKRERRRI-EQL 120
           AI+ S FGVGAMEIA+AEAEESAVT       PP P DL+ HAD+EEIAK+ER+RI EQL
Sbjct: 61  AIVASMFGVGAMEIAYAEAEESAVTPPPPPPPPPPPKDLSTHADLEEIAKKERQRINEQL 120

Query: 121 IRNKGLKYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDML 180
           IRNKG+KYGACPRFTVGVKGQKV+IKFQVPSSCEVSHLVAN+VSNLGLKVEERAGGSDML
Sbjct: 121 IRNKGMKYGACPRFTVGVKGQKVSIKFQVPSSCEVSHLVANLVSNLGLKVEERAGGSDML 180

Query: 181 LRAWDSPVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIK 240
           LRAW SPVAWQL+LT PKTQREAGGNEG S+E+DA D DLSVLIFHSL++SDKTEIEF+K
Sbjct: 181 LRAWASPVAWQLSLTRPKTQREAGGNEGNSLEIDAGDEDLSVLIFHSLVSSDKTEIEFLK 240

Query: 241 QGSLSTKELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGL 300
           QGSLSTKELDSLVSVLQLAGGRLGES+SFE KSREGSTQ+   EKSISSLE+MGVK+YGL
Sbjct: 241 QGSLSTKELDSLVSVLQLAGGRLGESRSFERKSREGSTQLPDPEKSISSLESMGVKVYGL 300

Query: 301 DEPRVNSSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAV 360
           DE  VNSS NEISWDNIAGYDQQK EIEDTIL+TLHNPELFD+IAHGTR KFESNRPRAV
Sbjct: 301 DEQHVNSSKNEISWDNIAGYDQQKSEIEDTILLTLHNPELFDEIAHGTRRKFESNRPRAV 360

Query: 361 LFEGPPGTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAI 420
           LFEGPPGTGKTS ARVIANQA VPL+YVPLEVIMSKYYGESERLLG+VFSLANELS GAI
Sbjct: 361 LFEGPPGTGKTSSARVIANQAGVPLLYVPLEVIMSKYYGESERLLGQVFSLANELSTGAI 420

Query: 421 IFLDEVDSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISR 480
           IFLDEVDSFAI RDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISR
Sbjct: 421 IFLDEVDSFAIARDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISR 480

Query: 481 FDSMITFGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSL 540
           FD MITFGLPDERNRQEIA+QYAKQLTKPELNEFA+ TEGMSGRDIRDICQQAER   S 
Sbjct: 481 FDLMITFGLPDERNRQEIAAQYAKQLTKPELNEFAKNTEGMSGRDIRDICQQAER---SW 540

Query: 541 FGQIIRGQVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
             +I+RG+VSKTGEHGILPPLQEYI CAMSRRKALQTVDD+KIKDSN   KKTQ A
Sbjct: 541 ASKILRGKVSKTGEHGILPPLQEYIECAMSRRKALQTVDDHKIKDSNIGMKKTQFA 593

BLAST of MS010745 vs. ExPASy TrEMBL
Match: A0A1S3B059 (katanin p60 ATPase-containing subunit A1 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484638 PE=3 SV=1)

HSP 1 Score: 951.4 bits (2458), Expect = 1.7e-273
Identity = 493/589 (83.70%), Postives = 530/589 (89.98%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFKLKDHSSTSRSLYPRTFQPQTGYLQPRSVFNGRERGFLYP 60
           M++  RI RTARAWRI+ASFKLKD  S SRS Y R F  Q  +L+P  + NGRE  FLYP
Sbjct: 1   MSVFNRISRTARAWRILASFKLKDSPSASRSFYLRQFGHQPEHLKPGLLSNGREPCFLYP 60

Query: 61  AIIVSTFGVGAMEIAFAEAEESAVTPPQPNDLTNHADMEEIAKRERRR-IEQLIRNKGLK 120
           AI+    GVGAMEIA+AEAEESA TPP P DL+ HADME++AK+ER R IEQL RNKG K
Sbjct: 61  AIVAGMLGVGAMEIAYAEAEESAATPPPPKDLSTHADMEDVAKKERLRIIEQLNRNKGTK 120

Query: 121 YGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLGLKVEERAGGSDMLLRAWDSP 180
           YGACPRFTVGVKGQKV+IKFQVP SCEVSHL+AN+VSNLGLKVEE AGGSDMLLRAWDSP
Sbjct: 121 YGACPRFTVGVKGQKVSIKFQVPPSCEVSHLIANLVSNLGLKVEESAGGSDMLLRAWDSP 180

Query: 181 VAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHSLITSDKTEIEFIKQGSLSTK 240
           VAWQLTL+ PK+QR AG N+G SIEMDAEDGDL+VLIFHSL+TSDKTEIEFIKQGSLSTK
Sbjct: 181 VAWQLTLSRPKSQRVAGENKGNSIEMDAEDGDLTVLIFHSLVTSDKTEIEFIKQGSLSTK 240

Query: 241 ELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSISSLEAMGVKIYGLDEPRVNS 300
           ELDSLVSVLQLAGGRLGES+S E KSREGSTQM SSEKSISSLEAMGVK+YGLDEP VNS
Sbjct: 241 ELDSLVSVLQLAGGRLGESRSSERKSREGSTQMPSSEKSISSLEAMGVKVYGLDEPHVNS 300

Query: 301 SNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPG 360
           + NEISWDNIAGYDQQKREIED+ILMTLHNPELFDDIAHGTR +FESNRPRAVLFEGPPG
Sbjct: 301 TENEISWDNIAGYDQQKREIEDSILMTLHNPELFDDIAHGTRRRFESNRPRAVLFEGPPG 360

Query: 361 TGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVD 420
           TGKTS ARVIANQA VPLVYVPLEVIMSKYYGESERLLGKVFSLANELS GAIIFLDEVD
Sbjct: 361 TGKTSSARVIANQAGVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSTGAIIFLDEVD 420

Query: 421 SFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALISRFDSMITF 480
           SFAI+RDSE+HEATRRVLSVLLRQIDGFEQDRKV+VIAATNRKQDLDPALISRFD MITF
Sbjct: 421 SFAISRDSEIHEATRRVLSVLLRQIDGFEQDRKVIVIAATNRKQDLDPALISRFDLMITF 480

Query: 481 GLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERFLFSLFGQIIRG 540
           GLPDERNR+EIA QYAKQLTKPEL EFAR T+GMSGRDIRDICQQAER   S   +IIRG
Sbjct: 481 GLPDERNREEIAVQYAKQLTKPELKEFARNTDGMSGRDIRDICQQAER---SWASKIIRG 540

Query: 541 QVSKTGEHGILPPLQEYIVCAMSRRKALQTVDDNKIKDSNSRTKKTQLA 589
           +VSKTGEHGILPP++EYI CAM RRKALQT+DD++IKD N RTKKTQLA
Sbjct: 541 KVSKTGEHGILPPIEEYIECAMKRRKALQTIDDHEIKDPNIRTKKTQLA 586

BLAST of MS010745 vs. TAIR 10
Match: AT4G04180.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 649.0 bits (1673), Expect = 3.5e-186
Identity = 354/615 (57.56%), Postives = 456/615 (74.15%), Query Frame = 0

Query: 1   MTILERIVRTARAWRIIASFK---LKDHSSTSRSL--------YPRTFQPQTGYLQPRSV 60
           M IL+++ R +RAWR   S     +   S  +R L        + ++       L+  S 
Sbjct: 1   MAILDKLFRASRAWRGSLSHSKSMVPSQSPRARELRRCFHHGNFEQSNSKVNQVLRSCST 60

Query: 61  FNGRERGFLYPAIIVSTFGVGAMEIAFAEAEES----AVTPPQPNDLTNHADME------ 120
            N      + PA++ + F VG + +A+AE++E+    +  P  PND T+ A ++      
Sbjct: 61  LNDSPYFSMAPAVLGALFSVGVIGVAYAESDEANNDKSSAPIDPNDETSSAPIDPPPNYV 120

Query: 121 EIAKRERRRIEQLIRNKGLKYGACPRFTVGVKGQKVTIKFQVPSSCEVSHLVANIVSNLG 180
           +IAK+ER R+E+LI++KG +YG+ PRF V V+GQK+T+KFQVPS+CEV+ L++NI S LG
Sbjct: 121 DIAKKERARVEELIQSKGTQYGSYPRFNVAVRGQKITLKFQVPSTCEVAQLISNIGSQLG 180

Query: 181 LKVEERAGGSDMLLRAWDSPVAWQLTLTNPKTQREAGGNEGISIEMDAEDGDLSVLIFHS 240
           +KV +R GGSDMLLRAWD+PVAWQ+TL + + +++ G +E      D  D DL +LIF S
Sbjct: 181 VKVSDRTGGSDMLLRAWDNPVAWQITLRSVENKKKLGESE------DDSDDDLCILIFGS 240

Query: 241 LITSDKTEIEFIKQGSLSTKELDSLVSVLQLAGGRLGESKSFESKSREGSTQMSSSEKSI 300
           L+TSDK E+EFIK+GSL+T+EL++ VS L +AG + G++K   S+   GST+ SS++KSI
Sbjct: 241 LLTSDKVEVEFIKKGSLTTEELEAFVSALGVAGTKAGQNKGSGSR---GSTRDSSTDKSI 300

Query: 301 SSLEAMGVKIYGLDEPRVNSSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHG 360
           S LE+MGV+IYG+++P  + S +EISWDNIAGYDQQKREIEDTILM LH+PE++DDI  G
Sbjct: 301 SQLESMGVRIYGVNKPLGDDSMDEISWDNIAGYDQQKREIEDTILMALHSPEVYDDIVRG 360

Query: 361 TRHKFESNRPRAVLFEGPPGTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGK 420
           TR KFESNRPRAVLFEGPPGTGKTSCARVIANQA +PL+YVPLE +MSKYYGESERLLG 
Sbjct: 361 TRSKFESNRPRAVLFEGPPGTGKTSCARVIANQAGIPLLYVPLEAVMSKYYGESERLLGA 420

Query: 421 VFSLANELSDGAIIFLDEVDSFAITRDSEMHEATRRVLSVLLRQIDGFEQDRKVVVIAAT 480
           VFS ANEL DGAIIFLDE+D+FAI+RDSEMHEATRRVLSVLLRQIDGFEQ++KVVVIAAT
Sbjct: 421 VFSQANELPDGAIIFLDEIDAFAISRDSEMHEATRRVLSVLLRQIDGFEQEKKVVVIAAT 480

Query: 481 NRKQDLDPALISRFDSMITFGLPDERNRQEIASQYAKQLTKPELNEFARITEGMSGRDIR 540
           NRKQDLDPALISRFDSMI F LPD + RQEI +QYAKQL+KPEL + A+ TE MSGRDIR
Sbjct: 481 NRKQDLDPALISRFDSMIMFDLPDLQTRQEIIAQYAKQLSKPELVQLAQATEAMSGRDIR 540

Query: 541 DICQQAERFLFS------LFGQIIRGQVSKTGEHGI-LPPLQEYIVCAMSRRKALQTVDD 588
           D+CQ AER   S      + GQ+IR   +   E  I LPP+QEY+  A +RRK+L++V +
Sbjct: 541 DVCQGAERTWASKIINLYIVGQLIRRAKAGGEEQKITLPPIQEYLESAEARRKSLRSVTE 600

BLAST of MS010745 vs. TAIR 10
Match: AT1G45000.1 (AAA-type ATPase family protein )

HSP 1 Score: 145.2 bits (365), Expect = 1.7e-34
Identity = 93/233 (39.91%), Postives = 127/233 (54.51%), Query Frame = 0

Query: 304 ISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKT 363
           IS+  + G   Q RE+ ++I + L NPELF  +            P+ VL  GPPGTGKT
Sbjct: 136 ISYSAVGGLGDQIRELRESIELPLMNPELFLRVG--------IKPPKGVLLYGPPGTGKT 195

Query: 364 SCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAI 423
             AR IA+  D   + V    I+ KY GES RL+ ++F+ A E     IIF+DE+D+   
Sbjct: 196 LLARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYARE-HQPCIIFMDEIDAIGG 255

Query: 424 TRDSEMHEATR---RVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFDSMIT 483
            R SE   A R   R L  LL Q+DGF+Q  KV +I ATNR   LDPAL+   R D  I 
Sbjct: 256 RRFSEGTSADREIQRTLMELLNQLDGFDQLGKVKMIMATNRPDVLDPALLRPGRLDRKIE 315

Query: 484 FGLPDERNRQEIASQYAKQLTKP---ELNEFARITEGMSGRDIRDICQQAERF 529
             LP+E++R EI   +A  + K    +     ++ EG +G D+R+IC +A  F
Sbjct: 316 IPLPNEQSRMEILKIHASGIAKHGEIDYEAIVKLGEGFNGADLRNICTEAGMF 359

BLAST of MS010745 vs. TAIR 10
Match: AT5G43010.1 (regulatory particle triple-A ATPase 4A )

HSP 1 Score: 142.5 bits (358), Expect = 1.1e-33
Identity = 91/233 (39.06%), Postives = 126/233 (54.08%), Query Frame = 0

Query: 304 ISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKT 363
           IS+  + G   Q RE+ ++I + L NPELF  +            P+ VL  GPPGTGKT
Sbjct: 136 ISYSAVGGLGDQIRELRESIELPLMNPELFLRVG--------IKPPKGVLLYGPPGTGKT 195

Query: 364 SCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAI 423
             AR IA+  D   + V    I+ KY GES RL+ ++F+ A E     IIF+DE+D+   
Sbjct: 196 LLARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYARE-HQPCIIFMDEIDAIGG 255

Query: 424 TRDSEMHEATR---RVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFDSMIT 483
            R SE   A R   R L  LL Q+DGF+   KV +I ATNR   LDPAL+   R D  I 
Sbjct: 256 RRFSEGTSADREIQRTLMELLNQLDGFDNLGKVKMIMATNRPDVLDPALLRPGRLDRKIE 315

Query: 484 FGLPDERNRQEIASQYAKQLTKP---ELNEFARITEGMSGRDIRDICQQAERF 529
             LP+E++R +I   +A  + K    +     ++ EG +G D+R+IC +A  F
Sbjct: 316 IPLPNEQSRMDILKIHAAGIAKHGEIDYEAIVKLAEGFNGADLRNICTEAGMF 359

BLAST of MS010745 vs. TAIR 10
Match: AT4G27680.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 4.1e-33
Identity = 84/237 (35.44%), Postives = 136/237 (57.38%), Query Frame = 0

Query: 297 VNSSNNEISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEG 356
           +N  + ++ + +I G +  K+ + + +++ L  PELF   A+G          + VL  G
Sbjct: 74  INPDHIDVEFGSIGGLETIKQALYELVILPLKRPELF---AYGKL----LGPQKGVLLYG 133

Query: 357 PPGTGKTSCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLD 416
           PPGTGKT  A+ IA ++    + V +  +MSK++G++++L+  VFSLA +L   AIIF+D
Sbjct: 134 PPGTGKTMLAKAIAKESGAVFINVRVSNLMSKWFGDAQKLVSAVFSLAYKLQP-AIIFID 193

Query: 417 EVDSFAITRDSEMHEATRRVLSVLLRQIDGFEQD--RKVVVIAATNRKQDLDPALISRFD 476
           EV+SF   R S  HEA   + +  +   DGF  D   +V+V+AATNR  +LD A++ R  
Sbjct: 194 EVESFLGQRRSTDHEAMANMKTEFMALWDGFSTDPHARVMVLAATNRPSELDEAILRRLP 253

Query: 477 SMITFGLPDERNRQEIASQYAK-QLTKPEL--NEFARITEGMSGRDIRDICQQAERF 529
                G+PD R R EI     K +  +P++  +  AR+ EG +G DI ++C++A  F
Sbjct: 254 QAFEIGIPDRRERAEILKVTLKGERVEPDIDFDHIARLCEGYTGSDIFELCKKAAYF 302

BLAST of MS010745 vs. TAIR 10
Match: AT3G53230.1 (ATPase, AAA-type, CDC48 protein )

HSP 1 Score: 140.2 bits (352), Expect = 5.3e-33
Identity = 82/236 (34.75%), Postives = 133/236 (56.36%), Query Frame = 0

Query: 304 ISWDNIAGYDQQKREIEDTILMTLHNPELFDDIAHGTRHKFESNRPRAVLFEGPPGTGKT 363
           +SW++I G +  KRE+++T+   + +PE F+        KF  +  + VLF GPPG GKT
Sbjct: 478 VSWEDIGGLENVKRELQETVQYPVEHPEKFE--------KFGMSPSKGVLFYGPPGCGKT 537

Query: 364 SCARVIANQADVPLVYVPLEVIMSKYYGESERLLGKVFSLANELSDGAIIFLDEVDSFAI 423
             A+ IAN+     + +    +++ ++GESE  + ++F  A + S   ++F DE+DS A 
Sbjct: 538 LLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQ-SAPCVLFFDELDSIAT 597

Query: 424 TRDSEMHE---ATRRVLSVLLRQIDGFEQDRKVVVIAATNRKQDLDPALI--SRFDSMIT 483
            R + + +   A  RVL+ LL ++DG    + V +I ATNR   +DPAL+   R D +I 
Sbjct: 598 QRGNSVGDAGGAADRVLNQLLTEMDGMNAKKTVFIIGATNRPDIIDPALLRPGRLDQLIY 657

Query: 484 FGLPDERNRQEI------ASQYAKQLTKPELNEFARITEGMSGRDIRDICQQAERF 529
             LPDE +R +I       S  AK +   +L   A+ T+G SG DI +ICQ++ ++
Sbjct: 658 IPLPDEESRYQIFKSCLRKSPVAKDV---DLRALAKYTQGFSGADITEICQRSCKY 701