MS010743 (gene) Bitter gourd (TR) v1

Overview
NameMS010743
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionPyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta
Locationscaffold35: 1699538 .. 1704312 (+)
RNA-Seq ExpressionMS010743
SyntenyMS010743
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAATTTCTGTCGTCTACAATGGTGCTGCCGCCGTCAACTCCTCTCCCGCGCCGGTGCCCGGCCGGGTCTCCTCCGTGTATAGCGAAGTCCAGTCGAGTCGTATCGACCACAGTCTTCCTCTACCTTCTGTTCTCAGAAGTTCCTTTACGATCGTCGATGGGCCCCCGAGCTCTGCCGCTGGCAATCCAGGTACTCTGTTCTAGCATTTACTCTCGGGATCTATTTGTATGTTGGTGTTAGTAGATCATATTGTGTCTCTTTCTTTCGATCCTTGAGAAATCTGGTTGTCTGCCTGCTCAATCAGTAAATGAGCTTTCAGTTTCTTTTTAGCAATTCAAACGGGATGAATAATCGTGGAAGCAGTTGCTTAGAGTTTGGGTTTTAAGTTCACTGCATTCATATTTCTGTGTCTGTTTCTGATCTCACAGATCTTACGTGAATTTTTGCTACATGGGTCTTGTATCTCCTTCCATAATTCCATTTCGGTATGAAACTGGGACGAATACTAATATTTTAGGGGTTTTTTTTTTTTTTTTTGCGTTTGTTTTAATCCAGACGAGATAGCGAAGCTATTCCCTAATCTCTTCGGTCAGCCATCGGCAAAATTGGTGCCATGCGATTCCAGCAAAGTTCGACCGGATAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAATGTCATTTCTGGAATTTTTGGTAATGATCATTTTACATTTGTTGAATTTATTTTGATAATTTATATGCAAATCTACTTTAACTAAATGTTTCTTTTATTCTTTGGGTTTTAGTGATTTTAAGTCTGTATTGGAAACAGATTACTTGCAGGATCACGTCAAAGGCAGTGTATTATATGGCTTCAGGGGTGGCCCTGCTGGGATTATGAAATCTAAATATGTGGAGTTGACTTCAGAGTATATTTACCCATACAGAAATCAGGTAAACCCTAGAGAGCTTCTCAAGTTTGAAAGTTTGAATACTTCTAATGATGTTGAAATTGATGATGATTCTTGGTAAACAGGAAAAATAACATTATTAACATTTTGATTTTCCTTGTTTGCAAATTCTTTTCAGGGTGGGTTTGATATGATATGTAGTGGGAGGGACAAAATTGAAACTCCAGAGCAGGTGAGACTCTCTTTTGCCTCTCAAGGGTGTGACGTTTTGTCCATGTGACTTGGATAATGTTAGTATATATGGACTTTACTTACTTGAGTCGGGTGAATTTTTCAGTTCAAACAAGCTGAAGAAACAGTAAAAAAGCTTGATTTGGATGGGCTTGTTGTGATTGGTGGAGACGATTCAAACACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGTGCGTATGAAGATAATGTCATGTGTCTCCTTAAAACAACATTGGCACAGTGCTATATTTGGCGAAATTTAATTTTTTGTACATACATTGATTCAGTTGTTTCTATTGTATGTTCTGTGAAGTAATGCCCGTTCTTCTGGTCAGGGGTAAAAATTTGAAGACCCGGGTCATTGGTTGCCCCAAAACAATTGATGGAGATTTAAAATGCAAAGAGGTGCCTACAAGTTTTGGATTTGATACAGCTTGCAGGGTAATTAAACCATTTATCTCGCCTTGTTCACATGTTATTGAATTCTTAACGTGCGGGTAATTGCTTTTGATGTTATTTAATATATCATTGTGCTATGATTGTTCTAATGCTTCTTTCAAGTATTATTCTCGTTGTGAAAGTAAATATTAGTATCTTGAGGTATTTCGAAAGTGTTAACACATTTCCTCGAACATGAGATTTCTAACGAAGTAAAGCTATATATACTTGATATTACCTGCAGAGAGAAGAAAGTTGTTGTCTGGGTTTTACGGTTTGCCTAGATTTGACTGGCACTACTATGTTCATGTACCGCTACTAAAGAAGTTTATGTTTTCAATGTTTCAGATATATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTGTAAGTAAAATATTCAAGTTAAGAACCACAGTGTTGTCATTTACCTTATCACCATATTGTACCATGAAGGAAATCCGTTCTGTAATTGTTTGAAGTGGTGGTTCATTCCCTGGTTTAAGAGCAGCACCAAATGAATTTAAATAATAATTCTGTGATATAACTCATCCGATGCTGCAATTTGATGATCCTAGTTTCTAAATTCTCCCAAAATTGTAATATAGTCATCTATTTTCACCAAATGGATTAAAATGTCATGCTTTGTTTTCTATCCATGTATAATTTATTAGATGTTTGTCACCTCAAGAAGTGGCATTTATCGGTTGCCAATTTAAGTACATATTTCTATGTAAGTGCTGTAGCATGGGTGTGGATCACTTACATTGGTCGGTAATTTTTGCTTTGTTGTGTTTCTATTAGCATATTTTTGTATATTTAAATCTGCAGTTGTCCGGCTTATGGGGCGTGCTGCTTCACACATCACTCTAGAGTGTGCCTTGCAGACTCATCCAAACATTACTATCATTGGGGAAGAGGTACTATCAACCTCTCGTCTGCTGCATTTTTCAATTGTGCTACTTTTTTGGGAAGGGGAACAAGGTTTCATTATCCAAATGGTGTCTTCACATTCTCTTCTTGCTATTGTAAATAATGCTAAATGCTCGTTTCATGACATATTTTAACATATCAAGCCTCTTAGTTAGATTATGATTGATCGAGAAACTTTGGTAGATGGTTATGAATTTTGCTGCATGTATAGTGTACCAACAACTAGGTTTTACGTTGCTGTAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTTACAGATTACATTGTAGATGTTATCTGCAAACGTGCAGAACTTGGTTATAACTATGGTGTTATACTTATTCCTGAAGGTCTTATTGACTTCATCCCAGAGGTATTTTCTTGCTCATAAAATCTGTAATGGTTGTCTTCTTACCCTCATTATTAGCTGTGTGCTTAATGATTATATGTTCTTAAATGCCTTACAGGTACAACAACTTATTGCAGAACTAAATGAAATCTTGGCTCATGATATTGTTGATGATGATGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGTTTTTGCCTCAAGCAATCCAAGAACAGTTGATGCTTGAAAGAGATCCACATGGAAATGTTCAGGTACAGTTATTGTATCTTTCTGTTTTTTGTTGTGATTGCCAGGTGTAAGTGCCTCTTGGTTAAATATGATATTTTCCTTCTTTTCTTGTTTAGGTTGCTAGAATAGAAACAGAGAAAATGCTTATTCAGATGGTTGAGACGGAGTTGGAGAAGAGGAAGAGTGAAGGTGTATACAAAGGCCAATTTAAAGGGCAATCTCACTTTTTTGGGTAAGAATTCTTCACTTTATTTGAGGACTGCAGTTTTCTTTCTTTCTATCTCTTTCTTTTTCTTTTATGCTACAACATTTAGCAGCATGGGAAAATGAGAATGTTACAACAACCCCTTGTATAATTTCAACTTGCAGCTATGAAGGGAGATGTGGTCTACCAACCAATTTTGATTCCACATACTGTTATGCATTGGGTTATGGTGCTGGAGCTCTTCTGCAGAGTGGAAAAACTGGACTAATATCATCGGTCTGTCCTGTTCTCTGCTCATATACCATCACTGCCCTCTGCAAACTCTTCAATAAAATATCAGTTTCCTTTACTCCTAATTTTGCTTGCAATATGTTTTCCCTATAGTTGGTAAATCTTTGTGCATATTCTGTGTTATTTACCAGGTGGGGAATTTAGCTGCGCCTGTTGAAGAGTGGACTGTTGGTGGTACCGCATTGACTTCATTAATGGATGTGGAGAGAAGACATGGTAGACTACAACTTGCTATCTCAGACTCTTTTATCTCCCTCATGCAGGGTCTAAATTTTAGTTTTTGTTTTCTTGTGTGGCCTCTTTTTTTTTTCCTTCCCTATTCTGCATGCTCCCCGAAATGGTTTGTTCATCTCCATAAAGAATATGATTGACAACTCTAAGCTTCAGGTTGTGTTCCTTTCTCGTAATATATGGCTATAAGCTTTTTCCCGAGGGTTCTTGAATCTTAGTCTAATATATTATTTACCTGGGACTGTTCAAATGATTTGATTTTCACGTACTTGTATTGCTACTGAAGTTGTATTTTTGTGACAGGCAAGTTTAAACCTGTGATTAAAAAGGCAATGGTGGAGCTTGATGGTAAGGACTGTGAAAGGAAACATTGTTTTTTGAGTAATATCATCACTAGATTAGTATCCTACTGTTGTCAATCACTTTCATGAACTTATCTGCAACTGGAAAAAACTAAAAATAAAGTTCGATTCATGAATTATCAGGTGCACCTTTCAAGAAGTTCGCGTCATTGAGGGATGAGTGGGCATTCAACAACCGATACATCAGTCCTGGTTAGTAAACATTCAACTTCTATCATTTTCTTGTTTCCTGCTCGCCATTCACGAAAGAGAACAATCTATTTCCTTGTCATGTTCCTCTTTTGGCTGCCTTTAATTTGTTTTTCCCTTTGCATTTGCTAATATTTAAATGTGGATAATTGAAAAAAAAAATGTTAATAATGCCTTAAAGAAAAAGAGCATGCACTGCTTCGATTAAATTATGACATTCCTCCTAATCGAAATTGCAGGTCCTATTCAATTTGTGGGTCCTGCTTCAAATGCTGTTAATCATACTCTCCTCCTGGAACTTGAAGCTGGAGCT

mRNA sequence

ATGTCAATTTCTGTCGTCTACAATGGTGCTGCCGCCGTCAACTCCTCTCCCGCGCCGGTGCCCGGCCGGGTCTCCTCCGTGTATAGCGAAGTCCAGTCGAGTCGTATCGACCACAGTCTTCCTCTACCTTCTGTTCTCAGAAGTTCCTTTACGATCGTCGATGGGCCCCCGAGCTCTGCCGCTGGCAATCCAGACGAGATAGCGAAGCTATTCCCTAATCTCTTCGGTCAGCCATCGGCAAAATTGGTGCCATGCGATTCCAGCAAAGTTCGACCGGATAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAATGTCATTTCTGGAATTTTTGATTACTTGCAGGATCACGTCAAAGGCAGTGTATTATATGGCTTCAGGGGTGGCCCTGCTGGGATTATGAAATCTAAATATGTGGAGTTGACTTCAGAGTATATTTACCCATACAGAAATCAGGGTGGGTTTGATATGATATGTAGTGGGAGGGACAAAATTGAAACTCCAGAGCAGTTCAAACAAGCTGAAGAAACAGTAAAAAAGCTTGATTTGGATGGGCTTGTTGTGATTGGTGGAGACGATTCAAACACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGGGTAAAAATTTGAAGACCCGGGTCATTGGTTGCCCCAAAACAATTGATGGAGATTTAAAATGCAAAGAGGTGCCTACAAGTTTTGGATTTGATACAGCTTGCAGGATATATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTCCGGCTTATGGGGCGTGCTGCTTCACACATCACTCTAGAGTGTGCCTTGCAGACTCATCCAAACATTACTATCATTGGGGAAGAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTTACAGATTACATTGTAGATGTTATCTGCAAACGTGCAGAACTTGGTTATAACTATGGTGTTATACTTATTCCTGAAGGTCTTATTGACTTCATCCCAGAGGTACAACAACTTATTGCAGAACTAAATGAAATCTTGGCTCATGATATTGTTGATGATGATGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGTTTTTGCCTCAAGCAATCCAAGAACAGTTGATGCTTGAAAGAGATCCACATGGAAATGTTCAGGTTGCTAGAATAGAAACAGAGAAAATGCTTATTCAGATGGTTGAGACGGAGTTGGAGAAGAGGAAGAGTGAAGGTGTATACAAAGGCCAATTTAAAGGGCAATCTCACTTTTTTGGCTATGAAGGGAGATGTGGTCTACCAACCAATTTTGATTCCACATACTGTTATGCATTGGGTTATGGTGCTGGAGCTCTTCTGCAGAGTGGAAAAACTGGACTAATATCATCGGTGGGGAATTTAGCTGCGCCTGTTGAAGAGTGGACTGTTGGTGGTACCGCATTGACTTCATTAATGGATGTGGAGAGAAGACATGGCAAGTTTAAACCTGTGATTAAAAAGGCAATGGTGGAGCTTGATGGTGCACCTTTCAAGAAGTTCGCGTCATTGAGGGATGAGTGGGCATTCAACAACCGATACATCAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCTTCAAATGCTGTTAATCATACTCTCCTCCTGGAACTTGAAGCTGGAGCT

Coding sequence (CDS)

ATGTCAATTTCTGTCGTCTACAATGGTGCTGCCGCCGTCAACTCCTCTCCCGCGCCGGTGCCCGGCCGGGTCTCCTCCGTGTATAGCGAAGTCCAGTCGAGTCGTATCGACCACAGTCTTCCTCTACCTTCTGTTCTCAGAAGTTCCTTTACGATCGTCGATGGGCCCCCGAGCTCTGCCGCTGGCAATCCAGACGAGATAGCGAAGCTATTCCCTAATCTCTTCGGTCAGCCATCGGCAAAATTGGTGCCATGCGATTCCAGCAAAGTTCGACCGGATAAGAAGTTGAAGATTGGTGTCGTGTTGTCTGGAGGCCAGGCCCCCGGAGGACATAATGTCATTTCTGGAATTTTTGATTACTTGCAGGATCACGTCAAAGGCAGTGTATTATATGGCTTCAGGGGTGGCCCTGCTGGGATTATGAAATCTAAATATGTGGAGTTGACTTCAGAGTATATTTACCCATACAGAAATCAGGGTGGGTTTGATATGATATGTAGTGGGAGGGACAAAATTGAAACTCCAGAGCAGTTCAAACAAGCTGAAGAAACAGTAAAAAAGCTTGATTTGGATGGGCTTGTTGTGATTGGTGGAGACGATTCAAACACAAATGCCTGCCTTCTTGCTGAAAACTTCAGGGGTAAAAATTTGAAGACCCGGGTCATTGGTTGCCCCAAAACAATTGATGGAGATTTAAAATGCAAAGAGGTGCCTACAAGTTTTGGATTTGATACAGCTTGCAGGATATATGCAGAAATGATTGGTAATGTCATGATAGATGCTAGATCAACTGGAAAATATTATCATTTTGTCCGGCTTATGGGGCGTGCTGCTTCACACATCACTCTAGAGTGTGCCTTGCAGACTCATCCAAACATTACTATCATTGGGGAAGAGGTTTCTGCTCAAAAGCAAACGCTGAAAAGTGTTACAGATTACATTGTAGATGTTATCTGCAAACGTGCAGAACTTGGTTATAACTATGGTGTTATACTTATTCCTGAAGGTCTTATTGACTTCATCCCAGAGGTACAACAACTTATTGCAGAACTAAATGAAATCTTGGCTCATGATATTGTTGATGATGATGGATTATGGAAAAAGAAACTCACTAGTCAATCTCTTGAACTTTTTGAGTTTTTGCCTCAAGCAATCCAAGAACAGTTGATGCTTGAAAGAGATCCACATGGAAATGTTCAGGTTGCTAGAATAGAAACAGAGAAAATGCTTATTCAGATGGTTGAGACGGAGTTGGAGAAGAGGAAGAGTGAAGGTGTATACAAAGGCCAATTTAAAGGGCAATCTCACTTTTTTGGCTATGAAGGGAGATGTGGTCTACCAACCAATTTTGATTCCACATACTGTTATGCATTGGGTTATGGTGCTGGAGCTCTTCTGCAGAGTGGAAAAACTGGACTAATATCATCGGTGGGGAATTTAGCTGCGCCTGTTGAAGAGTGGACTGTTGGTGGTACCGCATTGACTTCATTAATGGATGTGGAGAGAAGACATGGCAAGTTTAAACCTGTGATTAAAAAGGCAATGGTGGAGCTTGATGGTGCACCTTTCAAGAAGTTCGCGTCATTGAGGGATGAGTGGGCATTCAACAACCGATACATCAGTCCTGGTCCTATTCAATTTGTGGGTCCTGCTTCAAATGCTGTTAATCATACTCTCCTCCTGGAACTTGAAGCTGGAGCT

Protein sequence

MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGPASNAVNHTLLLELEAGA
Homology
BLAST of MS010743 vs. NCBI nr
Match: XP_022132924.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Momordica charantia])

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNAVNHTLLLELEAGA
Sbjct: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 567

BLAST of MS010743 vs. NCBI nr
Match: XP_022950848.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita moschata])

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 544/567 (95.94%), Postives = 555/567 (97.88%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+PAPV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. NCBI nr
Match: KAG7033973.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1096.6 bits (2835), Expect = 0.0e+00
Identity = 543/567 (95.77%), Postives = 554/567 (97.71%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+PAPV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+P KKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPGKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. NCBI nr
Match: KAG6603802.1 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 542/567 (95.59%), Postives = 553/567 (97.53%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+PAPV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+P KKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPNDSNKVQPGKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
            AQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 YAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. NCBI nr
Match: XP_023544353.1 (pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 542/567 (95.59%), Postives = 553/567 (97.53%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+ APV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNLAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPGDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKN KTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNWKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. ExPASy Swiss-Prot
Match: Q41141 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus communis OX=3988 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 4.2e-282
Identity = 474/545 (86.97%), Postives = 504/545 (92.48%), Query Frame = 0

Query: 19  PVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKLFPNLFGQP 78
           P  GR +SVYSEVQSSRI+H LPLPSVL   F IV GPPSSAAGNPDEIAKLFPNLFGQP
Sbjct: 4   PNSGRAASVYSEVQSSRIEHVLPLPSVLNHPFKIVQGPPSSAAGNPDEIAKLFPNLFGQP 63

Query: 79  SAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPA 138
           SA LVP  +  +  +++LKIG+VLSGGQAPGGHNVISGIFDYLQD  KGS+LYGFRGGPA
Sbjct: 64  SAMLVPDVADSLDSNQQLKIGLVLSGGQAPGGHNVISGIFDYLQDRAKGSILYGFRGGPA 123

Query: 139 GIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGG 198
           GIMK  YV+LT++YI+PYRNQGGFDMICSGRDKIETPEQFKQAEET  KLDL+GLVVIGG
Sbjct: 124 GIMKCNYVQLTADYIHPYRNQGGFDMICSGRDKIETPEQFKQAEETAGKLDLNGLVVIGG 183

Query: 199 DDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVM 258
           DDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTAC+IY+EMIGNVM
Sbjct: 184 DDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACKIYSEMIGNVM 243

Query: 259 IDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVI 318
           IDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV+A+K  LK VTDYIVDVI
Sbjct: 244 IDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVAAKKLALKDVTDYIVDVI 303

Query: 319 CKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKLTSQSLELF 378
           CKRA+LGYNYGVILIPEGLIDFIPEVQ LIAELNEILAHD+VD+ GLWKKKLTSQSL+LF
Sbjct: 304 CKRADLGYNYGVILIPEGLIDFIPEVQNLIAELNEILAHDVVDEGGLWKKKLTSQSLQLF 363

Query: 379 EFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKGQFKGQSHFF 438
           EFLP AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEKRK +G YK  FKGQSHFF
Sbjct: 364 EFLPVAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKRKQQGTYKAHFKGQSHFF 423

Query: 439 GYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLM 498
           GYEGRCGLPTNFDSTYCYALGY AGALL SGKTGLISSVGNL APV EWTVGGTALTSLM
Sbjct: 424 GYEGRCGLPTNFDSTYCYALGYAAGALLHSGKTGLISSVGNLGAPVAEWTVGGTALTSLM 483

Query: 499 DVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGPASNAVNHT 558
           DVERRHGKFKPVIKKAMVEL+GAPFKKFASLR+EWA  NRY+SPGPIQF+GP S+A +HT
Sbjct: 484 DVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYVSPGPIQFMGPGSDAASHT 543

Query: 559 LLLEL 564
           LLLEL
Sbjct: 544 LLLEL 548

BLAST of MS010743 vs. ExPASy Swiss-Prot
Match: P21343 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum tuberosum OX=4113 GN=PFP-BETA PE=1 SV=3)

HSP 1 Score: 968.0 bits (2501), Expect = 4.7e-281
Identity = 474/566 (83.75%), Postives = 515/566 (90.99%), Query Frame = 0

Query: 2   SISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAA 61
           ++S++ NG  A +S  +P  GR ++VYSEVQ+SR+DH LPLPSVL S F +VDGPPSSAA
Sbjct: 5   TLSLLNNGELA-SSVKSPGTGRYAAVYSEVQNSRLDHPLPLPSVLGSPFKVVDGPPSSAA 64

Query: 62  GNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           G+P+EIAKLFP+L+GQP   LVP DS  V  ++ LKIGVVLSGGQAPGGHNVISGIFDYL
Sbjct: 65  GHPEEIAKLFPSLYGQPCVSLVPDDSGDVAMNQILKIGVVLSGGQAPGGHNVISGIFDYL 124

Query: 122 QDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQA 181
           Q H KGS +YGFRGGPAG+MK KYV LT E+IYPYRNQGGFDMICSGRDKIETPEQFKQA
Sbjct: 125 QTHCKGSTMYGFRGGPAGVMKGKYVVLTPEFIYPYRNQGGFDMICSGRDKIETPEQFKQA 184

Query: 182 EETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSF 241
           EET KKLDLDGLVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLK KEVPTSF
Sbjct: 185 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKSKEVPTSF 244

Query: 242 GFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 301
           GFDTAC+IYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPN+T+IGEEV 
Sbjct: 245 GFDTACKIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNVTLIGEEVF 304

Query: 302 AQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVD 361
           A+K TLK+VTDYI DV+CKRAE GYNYGVILIPEGLIDFIPEVQQLIAELNEILAHD+VD
Sbjct: 305 AKKLTLKNVTDYIADVVCKRAESGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDVVD 364

Query: 362 DDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKR 421
           + G+WKKKLT Q LELFE LP AIQEQL+LERDPHGNVQVA+IETEKMLIQMVETEL++R
Sbjct: 365 EAGVWKKKLTPQCLELFELLPLAIQEQLLLERDPHGNVQVAKIETEKMLIQMVETELDQR 424

Query: 422 KSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLA 481
           K +G Y  QFKGQSHFFGYEGRCGLP+NFDSTYCYALGYGAG+LLQSGKTGLISSVGNLA
Sbjct: 425 KQKGAYNAQFKGQSHFFGYEGRCGLPSNFDSTYCYALGYGAGSLLQSGKTGLISSVGNLA 484

Query: 482 APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYIS 541
           APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVEL+GAPFKKFAS R+EWA NNRYI+
Sbjct: 485 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASKREEWALNNRYIN 544

Query: 542 PGPIQFVGPASNAVNHTLLLELEAGA 568
           PGPIQFVGP +N VNHTLLLEL   A
Sbjct: 545 PGPIQFVGPVANKVNHTLLLELGVDA 569

BLAST of MS010743 vs. ExPASy Swiss-Prot
Match: Q8W4M5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA1 PE=1 SV=1)

HSP 1 Score: 961.8 bits (2485), Expect = 3.3e-279
Identity = 474/564 (84.04%), Postives = 515/564 (91.31%), Query Frame = 0

Query: 2   SISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAA 61
           +++V  +  A  +   AP  GR +SVYSEVQSSRI+++LPLPSVL+ +F IV+GP SSAA
Sbjct: 4   ALAVTRDLTAVGSPENAPAKGR-ASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 63

Query: 62  GNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +VP D        KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 64  GNPDEIAKLFPGLYGQPSVAVVP-DQDAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 123

Query: 122 QDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQA 181
           Q+  KGS  YGF+GGPAGIMK KYVEL +EYI PYRNQGGFDMICSGRDKIETP+QFKQA
Sbjct: 124 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 183

Query: 182 EETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSF 241
           EET KKLDLDGLVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVPTSF
Sbjct: 184 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 243

Query: 242 GFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 301
           GFDTAC+IY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS
Sbjct: 244 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 303

Query: 302 AQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVD 361
           AQKQTLK+VTDY+VDVICKRAELGYNYGVILIPEGLIDFIPEVQ+LIAELNEILA+++VD
Sbjct: 304 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 363

Query: 362 DDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKR 421
           ++GLWKKKLT QSL+LF+ LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEKR
Sbjct: 364 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 423

Query: 422 KSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLA 481
           K  G YKGQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 424 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 483

Query: 482 APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYIS 541
           APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVEL+GAPFKKFASLR+EWA  NRYIS
Sbjct: 484 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 543

Query: 542 PGPIQFVGPASNAVNHTLLLELEA 566
           PGPIQF GP S++++HTLLLEL A
Sbjct: 544 PGPIQFTGPGSDSLSHTLLLELGA 565

BLAST of MS010743 vs. ExPASy Swiss-Prot
Match: F4JGR5 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabidopsis thaliana OX=3702 GN=PFP-BETA2 PE=3 SV=1)

HSP 1 Score: 942.2 bits (2434), Expect = 2.7e-273
Identity = 460/557 (82.59%), Postives = 509/557 (91.38%), Query Frame = 0

Query: 11  AAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKL 70
           A ++ +P P   RV+SVYSEVQ+SRIDH+LPLPSV ++ F I+DGPPSS+AG+P+EI KL
Sbjct: 14  AGISINP-PTNSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKL 73

Query: 71  FPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVL 130
           FPNLFGQPSA LVP  S++V  D+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L
Sbjct: 74  FPNLFGQPSALLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSL 133

Query: 131 YGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDL 190
           +GFRGGPAGIMK KY+ELTSE++YPYRNQGGFDMICSGRDKIETPEQFKQAEETV K+DL
Sbjct: 134 FGFRGGPAGIMKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDL 193

Query: 191 DGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIY 250
           DGLVVIGGDDSNTNACLLAE+FR KN+KT VIGCPKTIDGDLK KEVPTSFGFDTAC+IY
Sbjct: 194 DGLVVIGGDDSNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIY 253

Query: 251 AEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSV 310
           +EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV  +K TLK+V
Sbjct: 254 SEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNV 313

Query: 311 TDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKL 370
           TD IVDVI KRAE GYNYGVIL+PEGLIDFIPEVQQLI+ELNE+LA   VD++G WKK L
Sbjct: 314 TDNIVDVIYKRAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNL 373

Query: 371 TSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKGQ 430
             ++LE+FEFLPQ IQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK+K+EG Y+ +
Sbjct: 374 KKETLEIFEFLPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYERE 433

Query: 431 FKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVG 490
           F G+SHFFGYEGRCGLPTNFD+TYCYALGYGAG+LLQSGKTGLISSVGNLAAPVEEWTVG
Sbjct: 434 FMGKSHFFGYEGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVG 493

Query: 491 GTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGP 550
           GTALTSLMDVERRHGKFKPVIKKAMVEL+GAPFKKFAS R+EWA  NRYISPGPIQF GP
Sbjct: 494 GTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGP 553

Query: 551 ASNAVNHTLLLELEAGA 568
            S+A NHTL+LEL A A
Sbjct: 554 GSDARNHTLMLELGAQA 569

BLAST of MS010743 vs. ExPASy Swiss-Prot
Match: Q9EZ02 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermophila (strain ATCC 49972 / DSM 6192 / RI 19.B1) OX=665571 GN=pfp PE=1 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 3.1e-160
Identity = 286/550 (52.00%), Postives = 376/550 (68.36%), Query Frame = 0

Query: 29  SEVQSSRIDHSLPLPSVLRSSFTIVD---GPPSSAAGNPDEIAKLFPNLFGQPSAKLVPC 88
           S +Q +R  +   LP VL+     +    G P+ A  + +++ +LF N +G+P A L   
Sbjct: 5   SPLQKARYGYVPKLPPVLQDEIARIQAELGQPTEAVADREDLKRLFANTYGKPIATLT-- 64

Query: 89  DSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPAGIMKSKY 148
             S     ++  +GV+LSGG APGGHNVI G+FD L+   + S L GF+GGP+GI+  ++
Sbjct: 65  RGSNPEAGRRRTVGVILSGGPAPGGHNVICGLFDALKKANRESTLIGFKGGPSGILDDEW 124

Query: 149 VELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGGDDSNTNA 208
           +E T   I  YRN GGFD+I SGR KIETPEQF +A E  KK  LD LV+IGGDDSNTNA
Sbjct: 125 IEFTDSLINQYRNTGGFDIIGSGRTKIETPEQFAKALENAKKHGLDALVIIGGDDSNTNA 184

Query: 209 CLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVMIDARSTG 268
            LLAE F  +    +VIG PKTIDGDLK + +  SFGFDTA ++YAE+IGN+  DA S+ 
Sbjct: 185 ALLAEYFVQQGAPIQVIGIPKTIDGDLKNEYIEASFGFDTATKVYAELIGNIARDAISSR 244

Query: 269 KYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVICKRAELG 328
           KY+HF+RLMGR+ASHI LECALQTHPN+ I+ EEV  +  TL  + D IVD + KRA  G
Sbjct: 245 KYWHFIRLMGRSASHIALECALQTHPNVCIVSEEVREKNMTLSQIVDQIVDAVVKRAAKG 304

Query: 329 YNYGVILIPEGLIDFIPEVQQLIAELNEILAHDI-----VDDDG---LW-KKKLTSQSLE 388
            N+GV+L+PEGLI+FIPEV  LI ELN +LA +      +DD      W K KL+  +  
Sbjct: 305 ENFGVVLVPEGLIEFIPEVGALIDELNTLLAKEAEVFNRIDDPRERISWVKGKLSGNNQH 364

Query: 389 LFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKGQFKGQSH 448
           +F  LP+ IQ QL+++RDPHGNVQV+RIETEK+LI+MV + L+  K EG YKG+F   +H
Sbjct: 365 VFSSLPETIQAQLLMDRDPHGNVQVSRIETEKLLIEMVSSRLKALKEEGAYKGKFSALNH 424

Query: 449 FFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTS 508
           FFGYE RC  P+NFD+ YCYALG+ A  L+ +G TG I+++ NLA P  EW   G  LT 
Sbjct: 425 FFGYEARCAFPSNFDADYCYALGFTAFVLIANGLTGYIAAIKNLARPAVEWKPMGIPLTM 484

Query: 509 LMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGPASNAVN 567
           +M++E+RHGK KPVI+KA+V+L+GAPFK+FA+ RD WA  + Y+ PG IQ+ GP      
Sbjct: 485 MMNMEKRHGKMKPVIRKALVDLEGAPFKRFATQRDAWATESAYVFPGAIQYYGPDDVCNQ 544

BLAST of MS010743 vs. ExPASy TrEMBL
Match: A0A6J1BUG2 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Momordica charantia OX=3673 GN=LOC111005648 PE=3 SV=1)

HSP 1 Score: 1139.8 bits (2947), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNAVNHTLLLELEAGA
Sbjct: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 567

BLAST of MS010743 vs. ExPASy TrEMBL
Match: A0A6J1GG11 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111453824 PE=3 SV=1)

HSP 1 Score: 1099.3 bits (2842), Expect = 0.0e+00
Identity = 544/567 (95.94%), Postives = 555/567 (97.88%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+PAPV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNPAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVKGSVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKGSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. ExPASy TrEMBL
Match: A0A6J1IR64 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111478001 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 540/567 (95.24%), Postives = 553/567 (97.53%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+ APV GRVSSVYSEVQSSRIDH+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVRSNHAPVTGRVSSVYSEVQSSRIDHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DS+KV+PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSNKVQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDHVK SVLYGFRGGPAGIMK KYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHVKSSVLYGFRGGPAGIMKCKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDG+VVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGIVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVM+DARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMVDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
           SAQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLI+ELNEILAHDIV
Sbjct: 301 SAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLISELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWK+KLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKRKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RK+EG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKNEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLELEAGA 568
           SPGPIQFVGPASNA+NHTLLLEL A A
Sbjct: 541 SPGPIQFVGPASNAINHTLLLELGAEA 567

BLAST of MS010743 vs. ExPASy TrEMBL
Match: A0A5D3CRI6 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=PFP-BETA PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 538/563 (95.56%), Postives = 548/563 (97.34%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+  P+ GRVSSVYSEVQSSRI+H+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVKSNYPPLSGRVSSVYSEVQSSRINHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DSSK +PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSSKGQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDH KGSVLYGFRGGPAGIMK KY+ELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHAKGSVLYGFRGGPAGIMKCKYIELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
            AQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV
Sbjct: 301 YAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRD+WAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDDWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLEL 564
           SPGPIQFVGPASNAVNHTLLLEL
Sbjct: 541 SPGPIQFVGPASNAVNHTLLLEL 563

BLAST of MS010743 vs. ExPASy TrEMBL
Match: A0A1S3B0Y3 (Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis melo OX=3656 GN=LOC103484643 PE=3 SV=1)

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 538/563 (95.56%), Postives = 548/563 (97.34%), Query Frame = 0

Query: 1   MSISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSA 60
           MSIS + NG AAV S+  P+ GRVSSVYSEVQSSRI+H+LPLPSVLRSSFTIVDGPPSSA
Sbjct: 1   MSISFISNGEAAVKSNYPPLSGRVSSVYSEVQSSRINHNLPLPSVLRSSFTIVDGPPSSA 60

Query: 61  AGNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120
           AGNPDEIAKLFPNLFGQPSAKLVP DSSK +PDKKLKIGVVLSGGQAPGGHNVISGIFDY
Sbjct: 61  AGNPDEIAKLFPNLFGQPSAKLVPSDSSKGQPDKKLKIGVVLSGGQAPGGHNVISGIFDY 120

Query: 121 LQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180
           LQDH KGSVLYGFRGGPAGIMK KY+ELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ
Sbjct: 121 LQDHAKGSVLYGFRGGPAGIMKCKYIELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQ 180

Query: 181 AEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240
           AEET KKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS
Sbjct: 181 AEETAKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTS 240

Query: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300
           FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV
Sbjct: 241 FGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV 300

Query: 301 SAQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360
            AQKQTLKSVTDYIVDV+CKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV
Sbjct: 301 YAQKQTLKSVTDYIVDVVCKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIV 360

Query: 361 DDDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420
           DD+GLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK
Sbjct: 361 DDEGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEK 420

Query: 421 RKSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480
           RKSEG YK QFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL
Sbjct: 421 RKSEGAYKAQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNL 480

Query: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYI 540
           AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRD+WAFNNRYI
Sbjct: 481 AAPVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDDWAFNNRYI 540

Query: 541 SPGPIQFVGPASNAVNHTLLLEL 564
           SPGPIQFVGPASNAVNHTLLLEL
Sbjct: 541 SPGPIQFVGPASNAVNHTLLLEL 563

BLAST of MS010743 vs. TAIR 10
Match: AT1G12000.1 (Phosphofructokinase family protein )

HSP 1 Score: 961.8 bits (2485), Expect = 2.4e-280
Identity = 474/564 (84.04%), Postives = 515/564 (91.31%), Query Frame = 0

Query: 2   SISVVYNGAAAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAA 61
           +++V  +  A  +   AP  GR +SVYSEVQSSRI+++LPLPSVL+ +F IV+GP SSAA
Sbjct: 4   ALAVTRDLTAVGSPENAPAKGR-ASVYSEVQSSRINNTLPLPSVLKGAFKIVEGPASSAA 63

Query: 62  GNPDEIAKLFPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYL 121
           GNPDEIAKLFP L+GQPS  +VP D        KLKIGVVLSGGQAPGGHNVISG+FDYL
Sbjct: 64  GNPDEIAKLFPGLYGQPSVAVVP-DQDAPSSAPKLKIGVVLSGGQAPGGHNVISGLFDYL 123

Query: 122 QDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQA 181
           Q+  KGS  YGF+GGPAGIMK KYVEL +EYI PYRNQGGFDMICSGRDKIETP+QFKQA
Sbjct: 124 QERAKGSTFYGFKGGPAGIMKCKYVELNAEYIQPYRNQGGFDMICSGRDKIETPDQFKQA 183

Query: 182 EETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSF 241
           EET KKLDLDGLVVIGGDDSNTNACLLAENFR KNLKTRVIGCPKTIDGDLKCKEVPTSF
Sbjct: 184 EETAKKLDLDGLVVIGGDDSNTNACLLAENFRSKNLKTRVIGCPKTIDGDLKCKEVPTSF 243

Query: 242 GFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 301
           GFDTAC+IY+EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS
Sbjct: 244 GFDTACKIYSEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVS 303

Query: 302 AQKQTLKSVTDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVD 361
           AQKQTLK+VTDY+VDVICKRAELGYNYGVILIPEGLIDFIPEVQ+LIAELNEILA+++VD
Sbjct: 304 AQKQTLKNVTDYMVDVICKRAELGYNYGVILIPEGLIDFIPEVQELIAELNEILANEVVD 363

Query: 362 DDGLWKKKLTSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKR 421
           ++GLWKKKLT QSL+LF+ LP+AIQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEKR
Sbjct: 364 ENGLWKKKLTEQSLKLFDLLPEAIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKR 423

Query: 422 KSEGVYKGQFKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLA 481
           K  G YKGQF GQSHFFGYEGRCGLPTNFD+TYCYALGYGAG LL SGKTGLISSVGNLA
Sbjct: 424 KQAGAYKGQFMGQSHFFGYEGRCGLPTNFDATYCYALGYGAGVLLNSGKTGLISSVGNLA 483

Query: 482 APVEEWTVGGTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYIS 541
           APVEEWTVGGTALT+LMDVERRHGKFKPVIKKAMVEL+GAPFKKFASLR+EWA  NRYIS
Sbjct: 484 APVEEWTVGGTALTALMDVERRHGKFKPVIKKAMVELEGAPFKKFASLREEWALKNRYIS 543

Query: 542 PGPIQFVGPASNAVNHTLLLELEA 566
           PGPIQF GP S++++HTLLLEL A
Sbjct: 544 PGPIQFTGPGSDSLSHTLLLELGA 565

BLAST of MS010743 vs. TAIR 10
Match: AT4G04040.1 (Phosphofructokinase family protein )

HSP 1 Score: 942.2 bits (2434), Expect = 1.9e-274
Identity = 460/557 (82.59%), Postives = 509/557 (91.38%), Query Frame = 0

Query: 11  AAVNSSPAPVPGRVSSVYSEVQSSRIDHSLPLPSVLRSSFTIVDGPPSSAAGNPDEIAKL 70
           A ++ +P P   RV+SVYSEVQ+SRIDH+LPLPSV ++ F I+DGPPSS+AG+P+EI KL
Sbjct: 14  AGISINP-PTNSRVTSVYSEVQASRIDHTLPLPSVFKTPFKIIDGPPSSSAGHPEEIEKL 73

Query: 71  FPNLFGQPSAKLVPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVL 130
           FPNLFGQPSA LVP  S++V  D+KLKIGVVLSGGQAPGGHNVI GIFDYLQ++ +GS L
Sbjct: 74  FPNLFGQPSALLVPNQSNEVSSDQKLKIGVVLSGGQAPGGHNVICGIFDYLQEYARGSSL 133

Query: 131 YGFRGGPAGIMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDL 190
           +GFRGGPAGIMK KY+ELTSE++YPYRNQGGFDMICSGRDKIETPEQFKQAEETV K+DL
Sbjct: 134 FGFRGGPAGIMKGKYIELTSEFVYPYRNQGGFDMICSGRDKIETPEQFKQAEETVTKMDL 193

Query: 191 DGLVVIGGDDSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIY 250
           DGLVVIGGDDSNTNACLLAE+FR KN+KT VIGCPKTIDGDLK KEVPTSFGFDTAC+IY
Sbjct: 194 DGLVVIGGDDSNTNACLLAEHFRAKNMKTLVIGCPKTIDGDLKSKEVPTSFGFDTACKIY 253

Query: 251 AEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSV 310
           +EMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEV  +K TLK+V
Sbjct: 254 SEMIGNVMIDARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVFEKKLTLKNV 313

Query: 311 TDYIVDVICKRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKL 370
           TD IVDVI KRAE GYNYGVIL+PEGLIDFIPEVQQLI+ELNE+LA   VD++G WKK L
Sbjct: 314 TDNIVDVIYKRAENGYNYGVILVPEGLIDFIPEVQQLISELNEVLAEGNVDEEGQWKKNL 373

Query: 371 TSQSLELFEFLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKGQ 430
             ++LE+FEFLPQ IQEQLMLERDPHGNVQVA+IETEKMLIQMVETELEK+K+EG Y+ +
Sbjct: 374 KKETLEIFEFLPQTIQEQLMLERDPHGNVQVAKIETEKMLIQMVETELEKKKTEGTYERE 433

Query: 431 FKGQSHFFGYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVG 490
           F G+SHFFGYEGRCGLPTNFD+TYCYALGYGAG+LLQSGKTGLISSVGNLAAPVEEWTVG
Sbjct: 434 FMGKSHFFGYEGRCGLPTNFDATYCYALGYGAGSLLQSGKTGLISSVGNLAAPVEEWTVG 493

Query: 491 GTALTSLMDVERRHGKFKPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGP 550
           GTALTSLMDVERRHGKFKPVIKKAMVEL+GAPFKKFAS R+EWA  NRYISPGPIQF GP
Sbjct: 494 GTALTSLMDVERRHGKFKPVIKKAMVELEGAPFKKFASQREEWALKNRYISPGPIQFKGP 553

Query: 551 ASNAVNHTLLLELEAGA 568
            S+A NHTL+LEL A A
Sbjct: 554 GSDARNHTLMLELGAQA 569

BLAST of MS010743 vs. TAIR 10
Match: AT1G76550.1 (Phosphofructokinase family protein )

HSP 1 Score: 402.1 bits (1032), Expect = 7.2e-112
Identity = 221/549 (40.26%), Postives = 325/549 (59.20%), Query Frame = 0

Query: 29  SEVQSSRIDHSLPLPSVLRSSFTIV---DGPPSSAAGNPDEIAKLFPNLFGQPSAKLV-- 88
           S +Q  R  +   LP  L+ +   V   DG   + AG+   IA+ FP+  GQP A  +  
Sbjct: 12  SPLQQLRSQYHPELPPCLQGTTVRVELGDGTTVAKAGDAHIIARAFPHTLGQPLAHFLRA 71

Query: 89  ---PCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPAGI 148
                D+  +      ++G+V  G QAPGGHNV+ G+++ L+ H   + L GF GG  G+
Sbjct: 72  TAKVADAQIITEHPVKRVGIVFCGRQAPGGHNVVWGLYEALKVHNAKNTLLGFLGGSEGL 131

Query: 149 MKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGGDD 208
              K +E+T E +  Y+NQGG+DM+   +D+I T EQ   A +    L LD LV+IGG  
Sbjct: 132 FAQKTLEITDEVLQTYKNQGGYDMLGRTKDQIRTTEQVNAALKACTDLKLDSLVIIGGVT 191

Query: 209 SNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVMID 268
           SNT+A  LAE F      T+V+G P TI+GDLK + V  + GFDT C++ +++I N+  D
Sbjct: 192 SNTDAAHLAEFFAEAKCSTKVVGVPVTINGDLKNQFVEANVGFDTTCKVNSQLISNICTD 251

Query: 269 ARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVICK 328
           A S  KYY+FVRLMGR  SH+ LEC LQ+HPN+ I+GEEV+A K T+  +   I D +  
Sbjct: 252 ALSAEKYYYFVRLMGRKHSHVALECTLQSHPNMVILGEEVTASKLTIFDIIKQICDAVQA 311

Query: 329 RAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKLTSQSLELFEF 388
           RAE   N+GVILIPEGL++ IPE+  L+ E++ +L   +  D+     +L+S S  LFEF
Sbjct: 312 RAEQDKNHGVILIPEGLVESIPELYALLKEIHGLLKEGVQVDN--ISTQLSSWSSALFEF 371

Query: 389 LPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKG-QFKGQSHFFG 448
           LP  I++QL+L  +   + Q+++IETEK+L  +VETE+ KR  EG YKG +F    HFFG
Sbjct: 372 LPPFIKKQLLLHPESDDSAQLSQIETEKLLAYLVETEMNKRLKEGTYKGKKFNAICHFFG 431

Query: 449 YEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLMD 508
           Y+ R  LP+ FD  Y Y LG+    +L +G  G +++V NL +PV +W  G   ++++M 
Sbjct: 432 YQARGSLPSKFDCDYAYVLGHVCYHILAAGLNGYMATVTNLKSPVNKWKCGAAPISAMMT 491

Query: 509 VER---RHGKF---KPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGPASN 563
           V+R     G     +PVI  A V+L G  +        ++   + Y +PGP+Q+ GP ++
Sbjct: 492 VKRWSQNSGSTTIGRPVIHPASVDLKGKAYDLLRQNAQKFLMEDMYRNPGPVQYDGPGAD 551

BLAST of MS010743 vs. TAIR 10
Match: AT1G20950.1 (Phosphofructokinase family protein )

HSP 1 Score: 394.0 bits (1011), Expect = 2.0e-109
Identity = 221/552 (40.04%), Postives = 323/552 (58.51%), Query Frame = 0

Query: 29  SEVQSSRIDHSLPLPSVLRSSFTIV---DGPPSSAAGNPDEIAKLFPNLFGQPSAKL--- 88
           S +Q  R  +   LP  L+ +   V   DG   + A +   +A+ FP+  GQP A     
Sbjct: 12  SPLQQLRSQYKPELPPCLQGTTVRVELGDGTTVAEAADSHTMARAFPHTLGQPLAHFLRE 71

Query: 89  ---VPCDSSKVRPDKKLKIGVVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPAG 148
              VP D+  +     +++G+V  G QAPGGHNVI G+F+ L+ H   S L GF GG  G
Sbjct: 72  TAQVP-DAHIITELPSVRVGIVFCGRQAPGGHNVIWGLFEALKVHNAKSTLLGFLGGSEG 131

Query: 149 IMKSKYVELTSEYIYPYRNQGGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGGD 208
           +   K +E+T + +  Y+NQGG+D++   +D+I+T EQ   A +    L LDGLV+IGG 
Sbjct: 132 LFAQKTLEITDDILQTYKNQGGYDLLGRTKDQIKTTEQVNAALKACTDLKLDGLVIIGGV 191

Query: 209 DSNTNACLLAENFRGKNLKTRVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVMI 268
            SNT+A  LAE F      T+V+G P T +GDLK + V  + GFDT C++ +++I N   
Sbjct: 192 ISNTDAAHLAEFFAAAKCSTKVVGVPVTTNGDLKNQFVEANVGFDTICKVNSQLISNACT 251

Query: 269 DARSTGKYYHFVRLMGRAASHITLECALQTHPNITIIGEEVSAQKQTLKSVTDYIVDVIC 328
           DA S  KYY+F+RLMGR  SH+ LEC LQ+HPN+ I+GEEV+A K T+  +   I D + 
Sbjct: 252 DALSAEKYYYFIRLMGRKHSHVALECTLQSHPNMVILGEEVAASKLTIFDIAKQICDAVQ 311

Query: 329 KRAELGYNYGVILIPEGLIDFIPEVQQLIAELNEILAHDIVDDDGLWKKKLTSQSLELFE 388
            RA    N+GVILIPEGLI  IPEV  L+ E++ +L   +  D      +L+  S  LFE
Sbjct: 312 ARAVEDKNHGVILIPEGLIVSIPEVYALLKEIHGLLRQGVSADK--ISTQLSPWSSALFE 371

Query: 389 FLPQAIQEQLMLERDPHGNVQVARIETEKMLIQMVETELEKRKSEGVYKG-QFKGQSHFF 448
           FLP  I++QL+L  +   + Q+++IETEK+L  +VETE+ KR  EG YKG +F    HFF
Sbjct: 372 FLPPFIKKQLLLHPESDDSAQLSQIETEKLLAYLVETEMNKRLKEGTYKGKKFNAICHFF 431

Query: 449 GYEGRCGLPTNFDSTYCYALGYGAGALLQSGKTGLISSVGNLAAPVEEWTVGGTALTSLM 508
           GY+ R  LP+ FD  Y Y LG+    +L +G  G +++V NL +PV +W  G T +T++M
Sbjct: 432 GYQARGSLPSKFDCDYAYVLGHICYHILAAGLNGYMATVTNLKSPVNKWKCGATPITAMM 491

Query: 509 DVER--RHGKF------KPVIKKAMVELDGAPFKKFASLRDEWAFNNRYISPGPIQFVGP 563
            V+   +   +      +P I  AMV+L G  +        ++   + Y +PGP+Q+ GP
Sbjct: 492 TVKHWSQDASYTLTSIGRPAIHPAMVDLKGKAYDLLRQNAQKFLMEDLYRNPGPLQYDGP 551

BLAST of MS010743 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 85.1 bits (209), Expect = 2.0e-16
Identity = 54/188 (28.72%), Postives = 87/188 (46.28%), Query Frame = 0

Query: 100 VVLSGGQAPGGHNVISGIFDYLQDHVKGSVLYGFRGGPAGIMKSKYVELTSEYIYPYRNQ 159
           +V  GG  PG + VI  +   L        + G  GG  G      + L S+ +     +
Sbjct: 96  IVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAKNTIPLNSKVVNDIHKR 155

Query: 160 GGFDMICSGRDKIETPEQFKQAEETVKKLDLDGLVVIGGDDSNTNACLLAENFRGKNLKT 219
           GG  +I + R   +T     +  ++++   ++ + +IGGD +   A ++ E  R + LK 
Sbjct: 156 GG-TIIGTSRGGHDT----NKIVDSIQDRGINQVYIIGGDGTQRGASVIFEEIRRRRLKV 215

Query: 220 RVIGCPKTIDGDLKCKEVPTSFGFDTACRIYAEMIGNVMIDARSTGKYYHFVRLMGRAAS 279
            V+G PKTID D+    +  SFGFDTA       I    ++A S      FV+LMGR + 
Sbjct: 216 AVVGIPKTIDNDIPV--IDKSFGFDTAVEEAQRAINAAHVEAESNENGIGFVKLMGRYSG 275

Query: 280 HITLECAL 288
           +I +   L
Sbjct: 276 YIAMYATL 276

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132924.10.0e+00100.00pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Momordica... [more]
XP_022950848.10.0e+0095.94pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
KAG7033973.10.0e+0095.77Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
KAG6603802.10.0e+0095.59Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta, partial [... [more]
XP_023544353.10.0e+0095.59pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta [Cucurbita... [more]
Match NameE-valueIdentityDescription
Q411414.2e-28286.97Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Ricinus... [more]
P213434.7e-28183.75Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Solanum... [more]
Q8W4M53.3e-27984.04Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 1 OS=Arabi... [more]
F4JGR52.7e-27382.59Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta 2 OS=Arabi... [more]
Q9EZ023.1e-16052.00Pyrophosphate--fructose 6-phosphate 1-phosphotransferase OS=Spirochaeta thermoph... [more]
Match NameE-valueIdentityDescription
A0A6J1BUG20.0e+00100.00Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Momordi... [more]
A0A6J1GG110.0e+0095.94Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A6J1IR640.0e+0095.24Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucurbi... [more]
A0A5D3CRI60.0e+0095.56Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
A0A1S3B0Y30.0e+0095.56Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta OS=Cucumis... [more]
Match NameE-valueIdentityDescription
AT1G12000.12.4e-28084.04Phosphofructokinase family protein [more]
AT4G04040.11.9e-27482.59Phosphofructokinase family protein [more]
AT1G76550.17.2e-11240.26Phosphofructokinase family protein [more]
AT1G20950.12.0e-10940.04Phosphofructokinase family protein [more]
AT5G56630.12.0e-1628.72phosphofructokinase 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 325..337
score: 27.22
coord: 222..239
score: 30.2
coord: 189..205
score: 52.04
coord: 100..119
score: 32.56
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBPIRSFPIRSF005677PPi_PFK_PfpBcoord: 21..567
e-value: 1.3E-262
score: 870.1
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBTIGRFAMTIGR02477TIGR02477coord: 32..562
e-value: 2.2E-246
score: 816.4
IPR011183Pyrophosphate-dependent phosphofructokinase PfpBHAMAPMF_01980Phosphofructokinase_II_Longcoord: 27..565
score: 43.082813
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 97..462
e-value: 9.1E-38
score: 130.2
NoneNo IPR availableGENE3D1.10.10.480Phosphofructokinase; domain 3coord: 341..407
e-value: 6.5E-240
score: 798.9
NoneNo IPR availableGENE3D3.40.50.450coord: 41..516
e-value: 6.5E-240
score: 798.9
NoneNo IPR availablePANTHERPTHR43650:SF6PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASE SUBUNIT BETAcoord: 19..566
NoneNo IPR availablePANTHERPTHR43650PYROPHOSPHATE--FRUCTOSE 6-PHOSPHATE 1-PHOSPHOTRANSFERASEcoord: 19..566
IPR035966Phosphofructokinase superfamilyGENE3D3.40.50.460Phosphofructokinase domaincoord: 243..551
e-value: 6.5E-240
score: 798.9
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 30..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010743.1MS010743.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0006096 glycolytic process
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047334 diphosphate-fructose-6-phosphate 1-phosphotransferase activity