MS010704 (gene) Bitter gourd (TR) v1

Overview


Name	MS010704
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	DNA oxidative demethylase ALKBH2
Location	scaffold35: 1484128 .. 1485290 (-)
RNA-Seq Expression	MS010704
Synteny	MS010704

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGGTATTCCTCTTTCTCGATTCTCTTCTCTCTTTTCAGACGTTGTCAAATGTCATACTAATTAGAAATTGAAGATTTAACCAGTGACTGTTGGCTGTCTTGGATTATTTTACTGTTTGCCCGCCCTCTGCTTGAAATTAGGAAGCATTTTCACGCGATTGAGGTTGTCAGATTGAAGTTTTAATTTGCTAATGCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTAAGACTTTTCCTTTCAATCTTTCGCTATCAGGTTAATTTTGGCTTCTTTAGCTATAACTCCTCGTTACTAGTGGCTCCCCTGATTGTCTGAACTTTTCTCTGTCAAGGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAACAAGACTTCTCAACGTATTTGGTTTATTTCCATCTGTTTTTGTTGTCTCTGTTCAGATTTTGAAAAGCCCTTTTTCTCAAATCTTCAGTCTTTTGCTCAATTTCTTTCTCGCCATTTTAAAATTTATTTGATGTGGCTCCAGGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

mRNA sequence

ATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAACAAGACTTCTCAACGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

Coding sequence (CDS)

ATGACTTTGAGGTTGAAGGAAGTGACGGACTCAGAATCGGAGACGAGAATCCCTCGTCCTCTTGATTTGGGAAGACGACACACATTGGATCTCGGAAATGGAAGCGAAATCGTCTACATGACCAAGTTCGTGCCCTCCGATAAGGCTTGGACCTGGTTCGATTTCCTCAATCAACGCATTCCCTGGACCAGGCCTACCATTCGCGTCTTCGGACGCTCCCTACTCCAGCCTAGAGACACTTGCTACGTTGCGAGTCCAGGATTGACGACATTAACTTATAGTGGGTACAAACCACGTGCCTATACTTGGGATGATTTTCCTCCACTGAAGGAAATTTTGGACGCTGTCCACGAAGCTCTTCCTGGGAGTAGTTTTAATAGCTTACTCTTGAACAGGTATAAAGGTGGTAATGATTATGTGGGTTGGCATTCTGATGATGAGAAGCTGTATGCACTGAACCAGGAGATTGCTTCGGTATCGTTTGGATGTGAGCGAGATTTCTTGTTGAAAAAGAAGTCCAACAAGACTTCTCAACGAAGAAATGATGAGGAACCTCCAAGCAAGAAATCAAAGAAGAGCGGTGTGGCTGATACACACTCATTTGCTCTAAAGCACGGTTCACTTTTGGTTATGAAAGGCTACACTCAGCGGGATTGGATGCATTCGGTGCCTAAGCGCCTCAAGGCAGAGGCAACCCGCATTAACCTTACTTTCAGGCATGTTGTTGTTGAT

Protein sequence

MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVVD

Homology

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BLAST of MS010704 vs. NCBI nr
Match: XP_022133026.1 (DNA oxidative demethylase ALKBH2 [Momordica charantia])

HSP 1 Score: 505.4 bits (1300), Expect = 2.8e-139
Identity = 243/244 (99.59%), Postives = 243/244 (99.59%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI
Sbjct: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL
Sbjct: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248

Query: 241 VVVD 245
           VVVD
Sbjct: 249 VVVD 252

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BLAST of MS010704 vs. NCBI nr
Match: XP_038882915.1 (DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882917.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882918.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882919.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882920.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882921.1 DNA oxidative demethylase ALKBH2 [Benincasa hispida])

HSP 1 Score: 473.8 bits (1218), Expect = 9.0e-130
Identity = 223/244 (91.39%), Postives = 236/244 (96.72%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LRLKEVTDSESET+IPRP + GRR TLDLGNGSEIVY+ KFVPSD+AWTWFDFLNQRI
Sbjct: 1   MSLRLKEVTDSESETKIPRPFNSGRRQTLDLGNGSEIVYVPKFVPSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDFPPLK+ILDAVHEAL
Sbjct: 61  PWTRPTIRVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFPPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VAD+HSF LKHGSLLVM+GYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADSHSFVLKHGSLLVMRGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 VVVD 245
           V+VD
Sbjct: 241 VIVD 244

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BLAST of MS010704 vs. NCBI nr
Match: KAG6603836.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 464.5 bits (1194), Expect = 5.5e-127
Identity = 219/241 (90.87%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 1   MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 60

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 61  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 V 242
           V
Sbjct: 241 V 241

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BLAST of MS010704 vs. NCBI nr
Match: XP_022950477.1 (DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 DNA oxidative demethylase ALKBH2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 464.5 bits (1194), Expect = 5.5e-127
Identity = 219/241 (90.87%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 254

Query: 241 V 242
           V
Sbjct: 255 V 255

[Close]

BLAST of MS010704 vs. NCBI nr
Match: XP_004134811.1 (DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742940.1 DNA oxidative demethylase ALKBH2 [Cucumis sativus] >KGN49032.1 hypothetical protein Csa_003044 [Cucumis sativus])

HSP 1 Score: 464.2 bits (1193), Expect = 7.2e-127
Identity = 219/244 (89.75%), Postives = 232/244 (95.08%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEVTDSESET+IPRP DLG+R TLDLGNGSEI+Y++KFV SD+AWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTI VFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDF PLK+ILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VAD HSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 VVVD 245
           V+VD
Sbjct: 241 VIVD 244

[Close]

BLAST of MS010704 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-86
Identity = 147/219 (67.12%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 26  RHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVAS 85
           R T+DLG+GS+++Y+ +F+P  ++WT+FD+L++ IPWTRPTIRVFGRS LQPRDTCYVAS
Sbjct: 96  RKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQPRDTCYVAS 155

Query: 86  PGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSD 145
            GLT L YSGY+P +Y+WDDFPPLKEILDA+++ LPGS FNSLLLNRYKG +DYV WH+D
Sbjct: 156 SGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAWHAD 215

Query: 146 DEKLYALNQEIASVSFGCERDFLLKKKSNKTSQ--RRNDEEPPSKKSKKSGVADTHSFAL 205
           DEK+Y    EIASVSFGCERDF+LKKK ++ S   +  D  P  K+ K+S   D  S  L
Sbjct: 216 DEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQSLTL 275

Query: 206 KHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 243
           KHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTFR V+
Sbjct: 276 KHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

[Close]

BLAST of MS010704 vs. ExPASy Swiss-Prot
Match: Q58DM4 (DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.7e-22
Identity = 67/176 (38.07%), Postives = 92/176 (52.27%), Query Frame = 0


Query: 67  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 126
           ++VFG+    PR        GL T T+SG       W   P L+ + D V   + G +FN
Sbjct: 98  VQVFGKWHSVPRKQATYGDTGL-TYTFSGLTLSPKPW--IPVLERVRDRV-SLVTGQTFN 157

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  AL   IASVSFG  RDF+ + K ++          P
Sbjct: 158 FVLINRYKDGQDHIGEHRDDERELALGSPIASVSFGACRDFVFRHKDSRGKH-------P 217

Query: 187 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 243
           S++       +     L HGSLL+M   T   W HS+P R K  A R+NLTFR ++
Sbjct: 218 SRR------LEVVRLQLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKIL 256

[Close]

BLAST of MS010704 vs. ExPASy Swiss-Prot
Match: Q6P6J4 (DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.5e-21
Identity = 65/176 (36.93%), Postives = 90/176 (51.14%), Query Frame = 0


Query: 67  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 126
           ++VFG+    PR        GL T T+SG       W   P L+ + D V E + G +FN
Sbjct: 77  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLTPKPW--VPVLERVRDRVCE-VTGQTFN 136

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++  + R      
Sbjct: 137 FVLVNRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFIFRHKDSRGKRPRR----- 196

Query: 187 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 243
                     +     L HGSLL+M   T   W HS+P R +  A R+NLTFR ++
Sbjct: 197 --------TVEVVRLQLAHGSLLMMNPPTNTHWYHSLPIRKRVLAPRVNLTFRKIL 235

[Close]

BLAST of MS010704 vs. ExPASy Swiss-Prot
Match: Q6NS38 (DNA oxidative demethylase ALKBH2 OS=Homo sapiens OX=9606 GN=ALKBH2 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 4.2e-21
Identity = 69/177 (38.98%), Postives = 91/177 (51.41%), Query Frame = 0


Query: 67  IRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFN 126
           ++VFG+    PR        GL T T+SG       W   P L+ I D V   + G +FN
Sbjct: 99  VQVFGKWHSVPRKQATYGDAGL-TYTFSGLTLSPKPW--IPVLERIRDHV-SGVTGQTFN 158

Query: 127 SLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEPP 186
            +L+NRYK G D++G H DDE+  A    IASVSFG  RDF+ + K ++           
Sbjct: 159 FVLINRYKDGCDHIGEHRDDERELAPGSPIASVSFGACRDFVFRHKDSR----------- 218

Query: 187 SKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVV 244
             KS    VA      L HGSLL+M   T   W HS+P R K  A R+NLTFR +++
Sbjct: 219 -GKSPSRRVAVVR-LPLAHGSLLMMNHPTNTHWYHSLPVRKKVLAPRVNLTFRKILL 258

[Close]

BLAST of MS010704 vs. ExPASy Swiss-Prot
Match: Q32L00 (Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 GN=ALKBH3 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.2e-21
Identity = 78/239 (32.64%), Postives = 107/239 (44.77%), Query Frame = 0


Query: 12  ESETRIPRPLDLGRRHTLDLGNG----SEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTI 71
           E   R P P  + +    ++       S +     FV   +A + F+ L + +PW + T 
Sbjct: 61  EGVRRAPEPRVIEKEGVYEISMSPTGISRVCLCPGFVDLKEADSVFEQLCRDVPWKQRTG 120

Query: 72  RVFGRSLLQPRDTCYVASPGLTTLTYS--GYKPRAYTWDDFPPLKEILDAVHEALPGSSF 131
                +  QPR T +    G    TYS    +P  +    + P+  +L    E   G SF
Sbjct: 121 IRDDVTYQQPRLTAWY---GELPYTYSRITMEPNPH----WHPVLLMLKNQIEENTGHSF 180

Query: 132 NSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRRNDEEP 191
           NSLL N Y+   D V WHSDDE        IAS+SFG  R F ++KK            P
Sbjct: 181 NSLLCNLYRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRMFEMRKK------------P 240

Query: 192 PSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVVVD 245
           P + +      +     L HG+LL+M+G TQ DW H VPK   +   RINLTFR V  D
Sbjct: 241 PPEDNGDYTYVERVKIPLDHGTLLIMEGATQADWQHRVPKEYHSREPRINLTFRTVYPD 280

[Close]

BLAST of MS010704 vs. ExPASy TrEMBL
Match: A0A6J1BTV9 (DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 PE=4 SV=1)

HSP 1 Score: 505.4 bits (1300), Expect = 1.4e-139
Identity = 243/244 (99.59%), Postives = 243/244 (99.59%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI
Sbjct: 9   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 68

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL
Sbjct: 69  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 128

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 129 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKPNKTSQRR 188

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 189 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 248

Query: 241 VVVD 245
           VVVD
Sbjct: 249 VVVD 252

[Close]

BLAST of MS010704 vs. ExPASy TrEMBL
Match: A0A6J1GEX9 (DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 464.5 bits (1194), Expect = 2.7e-127
Identity = 219/241 (90.87%), Postives = 231/241 (95.85%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 254

Query: 241 V 242
           V
Sbjct: 255 V 255

[Close]

BLAST of MS010704 vs. ExPASy TrEMBL
Match: A0A0A0KL20 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510990 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 3.5e-127
Identity = 219/244 (89.75%), Postives = 232/244 (95.08%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEVTDSESET+IPRP DLG+R TLDLGNGSEI+Y++KFV SD+AWTWFDFLNQRI
Sbjct: 1   MSLRFKEVTDSESETKIPRPFDLGKRQTLDLGNGSEILYISKFVSSDQAWTWFDFLNQRI 60

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTI VFGRS+LQPRDTCYVA+PGLTTLTYSGYKP AYTWDDF PLK+ILDAVHEAL
Sbjct: 61  PWTRPTICVFGRSVLQPRDTCYVANPGLTTLTYSGYKPHAYTWDDFTPLKDILDAVHEAL 120

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGS FNSLLLNRYKGGNDYVGWHSDDEK+YALNQEIASVSFGCERDFLLKKK NKTSQRR
Sbjct: 121 PGSRFNSLLLNRYKGGNDYVGWHSDDEKVYALNQEIASVSFGCERDFLLKKKPNKTSQRR 180

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240
           NDEEPP+KKSKKS VAD HSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH
Sbjct: 181 NDEEPPAKKSKKSSVADRHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRH 240

Query: 241 VVVD 245
           V+VD
Sbjct: 241 VIVD 244

[Close]

BLAST of MS010704 vs. ExPASy TrEMBL
Match: A0A6J1GEX8 (DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453571 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 6.5e-126
Identity = 217/239 (90.79%), Postives = 229/239 (95.82%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYV +PGLTT +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTTYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 240
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of MS010704 vs. ExPASy TrEMBL
Match: A0A6J1IQU8 (DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.2e-125
Identity = 216/239 (90.38%), Postives = 228/239 (95.40%), Query Frame = 0


Query: 1   MTLRLKEVTDSESETRIPRPLDLGRRHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRI 60
           M+LR KEV DSESET+IPRPLDLGRR TLDLGNGSEIVY++KFV SD+AWTWFDFLNQ I
Sbjct: 15  MSLRFKEVIDSESETKIPRPLDLGRRQTLDLGNGSEIVYISKFVSSDQAWTWFDFLNQHI 74

Query: 61  PWTRPTIRVFGRSLLQPRDTCYVASPGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEAL 120
           PWTRPTIRVFGRS++QPRDTCYV +PGLT  +YSGYKP AYTWDDFPPLK+IL+AVHEAL
Sbjct: 75  PWTRPTIRVFGRSVVQPRDTCYVVNPGLTIYSYSGYKPHAYTWDDFPPLKDILNAVHEAL 134

Query: 121 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 180
           PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR
Sbjct: 135 PGSSFNSLLLNRYKGGNDYVGWHSDDEKLYALNQEIASVSFGCERDFLLKKKSNKTSQRR 194

Query: 181 NDEEPPSKKSKKSGVADTHSFALKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 240
           NDEEPP+KKSKKS VADTHSF LKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR
Sbjct: 195 NDEEPPAKKSKKSSVADTHSFVLKHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFR 253

[Close]

BLAST of MS010704 vs. TAIR 10
Match: AT2G22260.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 320.9 bits (821), Expect = 9.1e-88
Identity = 147/219 (67.12%), Postives = 179/219 (81.74%), Query Frame = 0


Query: 26  RHTLDLGNGSEIVYMTKFVPSDKAWTWFDFLNQRIPWTRPTIRVFGRSLLQPRDTCYVAS 85
           R T+DLG+GS+++Y+ +F+P  ++WT+FD+L++ IPWTRPTIRVFGRS LQPRDTCYVAS
Sbjct: 96  RKTIDLGHGSDLIYIQRFLPFQQSWTFFDYLDKHIPWTRPTIRVFGRSCLQPRDTCYVAS 155

Query: 86  PGLTTLTYSGYKPRAYTWDDFPPLKEILDAVHEALPGSSFNSLLLNRYKGGNDYVGWHSD 145
            GLT L YSGY+P +Y+WDDFPPLKEILDA+++ LPGS FNSLLLNRYKG +DYV WH+D
Sbjct: 156 SGLTALVYSGYRPTSYSWDDFPPLKEILDAIYKVLPGSRFNSLLLNRYKGASDYVAWHAD 215

Query: 146 DEKLYALNQEIASVSFGCERDFLLKKKSNKTSQ--RRNDEEPPSKKSKKSGVADTHSFAL 205
           DEK+Y    EIASVSFGCERDF+LKKK ++ S   +  D  P  K+ K+S   D  S  L
Sbjct: 216 DEKIYGPTPEIASVSFGCERDFVLKKKKDEESSQGKTGDSGPAKKRLKRSSREDQQSLTL 275

Query: 206 KHGSLLVMKGYTQRDWMHSVPKRLKAEATRINLTFRHVV 243
           KHGSLLVM+GYTQRDW+HSVPKR KAE TRINLTFR V+
Sbjct: 276 KHGSLLVMRGYTQRDWIHSVPKRAKAEGTRINLTFRLVL 314

The following BLAST results are available for this feature:

BLAST of MS010704 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133026.1	2.8e-139	99.59	DNA oxidative demethylase ALKBH2 [Momordica charantia]	[more]
XP_038882915.1	9.0e-130	91.39	DNA oxidative demethylase ALKBH2 [Benincasa hispida] >XP_038882916.1 DNA oxidati...	[more]
KAG6603836.1	5.5e-127	90.87	DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. sororia...	[more]
XP_022950477.1	5.5e-127	90.87	DNA oxidative demethylase ALKBH2 isoform X2 [Cucurbita moschata] >KAG7034017.1 D...	[more]
XP_004134811.1	7.2e-127	89.75	DNA oxidative demethylase ALKBH2 [Cucumis sativus] >XP_031742939.1 DNA oxidative...	[more]

BLAST of MS010704 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	1.3e-86	67.12	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]
Q58DM4	1.7e-22	38.07	DNA oxidative demethylase ALKBH2 OS=Bos taurus OX=9913 GN=ALKBH2 PE=2 SV=1	[more]
Q6P6J4	2.5e-21	36.93	DNA oxidative demethylase ALKBH2 OS=Mus musculus OX=10090 GN=Alkbh2 PE=1 SV=1	[more]
Q6NS38	4.2e-21	38.98	DNA oxidative demethylase ALKBH2 OS=Homo sapiens OX=9606 GN=ALKBH2 PE=1 SV=1	[more]
Q32L00	7.2e-21	32.64	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 OS=Bos taurus OX=9913 G...	[more]

BLAST of MS010704 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTV9	1.4e-139	99.59	DNA oxidative demethylase ALKBH2 OS=Momordica charantia OX=3673 GN=LOC111005717 ...	[more]
A0A6J1GEX9	2.7e-127	90.87	DNA oxidative demethylase ALKBH2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A0A0KL20	3.5e-127	89.75	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A6J1GEX8	6.5e-126	90.79	DNA oxidative demethylase ALKBH2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IQU8	3.2e-125	90.38	DNA oxidative demethylase ALKBH2 OS=Cucurbita maxima OX=3661 GN=LOC111477932 PE=...	[more]

BLAST of MS010704 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22260.1	9.1e-88	67.12	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 27..243 e-value: 7.4E-60 score: 204.3
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 37..239 e-value: 5.8E-32 score: 111.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..196
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 172..195
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 32..241
IPR032852	DNA oxidative demethylase ALKBH2	PANTHER	PTHR31573	ALPHA-KETOGLUTARATE-DEPENDENT DIOXYGENASE ALKB HOMOLOG 2	coord: 23..242
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 124..242 score: 12.683493

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010704.1	MS010704.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006281	DNA repair