MS010690 (gene) Bitter gourd (TR) v1

BLAST of MS010690 vs. NCBI nr
Match: XP_022132647.1 (E3 ubiquitin-protein ligase RGLG2-like, partial [Momordica charantia])

HSP 1 Score: 887.9 bits (2293), Expect = 3.7e-254
Identity = 427/432 (98.84%), Postives = 428/432 (99.07%), Query Frame = 0

Query: 13  FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS 72
           FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS
Sbjct: 1   FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS 60

Query: 73  NLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCYG 132
           NLIIGIDFTKSNEWTGKKSF GRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCY 
Sbjct: 61  NLIIGIDFTKSNEWTGKKSFTGRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCYS 120

Query: 133 FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS 192
           FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS
Sbjct: 121 FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS 180

Query: 193 DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSIILVGVGEGPWDQM 252
           DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYP SIILVGVG+GPWDQM
Sbjct: 181 DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPSSIILVGVGDGPWDQM 240

Query: 253 YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ 312
           YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ
Sbjct: 241 YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ 300

Query: 313 KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT 372
           KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT
Sbjct: 301 KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT 360

Query: 373 TPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC 432
            PSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC
Sbjct: 361 PPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC 420

Query: 433 QTQIETRIKLIN 445
           QTQIETRIKLIN
Sbjct: 421 QTQIETRIKLIN 432

BLAST of MS010690 vs. NCBI nr
Match: XP_038881533.1 (E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida] >XP_038881535.1 E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida])

HSP 1 Score: 615.1 bits (1585), Expect = 4.6e-172
Identity = 311/444 (70.05%), Postives = 338/444 (76.13%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MGG+PSK I  H H HG +Q Q S CSC    P S S+    + P Y  NGDDF+SLEQV
Sbjct: 1   MGGRPSKHIHTHVHIHGLNQEQPSKCSCGHSPPPSTSSGPSSQYPSYKINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           T AL +AGLESSNLIIGIDFTKSNEWTG KSFMGRNLHDL G   NPYEQAISIIGRTL 
Sbjct: 61  TDALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN IPCYGFGD TTGD++VFSFH +DRPCHGFEEVL CYRER PHVKLAGPTSF P
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPDDRPCHGFEEVLFCYRERVPHVKLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT  GH SPQEQSTIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTRPGHLSPQEQSTIDAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPW QM ECDDQIPAR FDNFQFVNFT IMS      +KETEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWGQMNECDDQIPARDFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YK T+ LQLLGQKR+T+   PFP+P PPP  N   N       SPSYRRT+SD+ ++ F 
Sbjct: 301 YKETMRLQLLGQKRETQEHLPFPLPRPPPFINNFRN------YSPSYRRTDSDITSASF- 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
              S P+S  H+  S          RS   S S SFNRICP C+  E+DL FGCGHQ CS
Sbjct: 361 ---SGPSSPAHSILS----------RSSTWSSSPSFNRICPKCYFNEKDLAFGCGHQTCS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKLIN 445
           DCGKDL FCP+CQTQI   IKL N
Sbjct: 421 DCGKDLIFCPTCQTQITKSIKLRN 424

BLAST of MS010690 vs. NCBI nr
Match: XP_008440952.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >KAA0025590.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa] >TYK12465.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa])

HSP 1 Score: 602.4 bits (1552), Expect = 3.1e-168
Identity = 303/444 (68.24%), Postives = 336/444 (75.68%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MGGKPSK I  H H H   + Q S CSC    P S S+   F+ P +  NGDDF+SLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           T AL +AGLESSNLIIGIDFTKSNEWTG KSFMGRNLHDL G   NPYEQAISIIGRTL 
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN+IPCYGFGD TTGD++VFSFH  DRPCHGFEEVL CYRE  P V LAGPTSF P
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR++DT  GHFSPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQM ECDD+IPAR+FDNFQFVNFT IMS      +KETEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YK T+ LQLLGQK++T+   PFP+P PPPI NI  N  P     PSYRRT+SD+ +S  G
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYP-----PSYRRTDSDISSSFSG 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
                            +P+ S   RS   S + SFNRICP C+  E++L FGCGHQ CS
Sbjct: 361 ---------------STSPAHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKLIN 445
           DCGKDL FCP+CQTQI   IKL N
Sbjct: 421 DCGKDLLFCPTCQTQITKSIKLRN 424

BLAST of MS010690 vs. NCBI nr
Match: XP_023544775.1 (E3 ubiquitin-protein ligase RGLG5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.0 bits (1538), Expect = 1.3e-166
Identity = 301/453 (66.45%), Postives = 337/453 (74.39%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSP-----------SSISTASIFRSPDYLK 60
           MG K SK I CH H +G  + Q   CSC    P            S ++A   R P+ + 
Sbjct: 1   MGAKSSKNIHCHVHLYGDHRNQPLRCSCGYSPPPTTSPWPSSPSPSTNSAPSSRPPNPII 60

Query: 61  NGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYE 120
           NGDDF+SLEQV+ AL +AGLESSNLI+GIDFTKSNEWTGKKSFMGRNLHDL G C NPYE
Sbjct: 61  NGDDFESLEQVSEALEKAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYE 120

Query: 121 QAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPH 180
           QAISIIG+TL +FD+DN IPCYGFGD TTGD+ VFSFH  D PCHGFEEVL CYRE  PH
Sbjct: 121 QAISIIGKTLEKFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPH 180

Query: 181 VKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAI 240
           VKLAGPTSF PIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDT  GH SPQEQSTI+AI
Sbjct: 181 VKLAGPTSFAPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAI 240

Query: 241 VEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEF 300
           VEASKYPLSIILVGVG+GPWDQMY CDDQIPAR FDNFQFVNFT+IMS   P  +K+TEF
Sbjct: 241 VEASKYPLSIILVGVGDGPWDQMYRCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEF 300

Query: 301 ALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRR 360
           ALQ LMEIPPQYKAT+ LQLLGQKR T+G  PFP+P PPPI  IS N        P++RR
Sbjct: 301 ALQCLMEIPPQYKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRN------HFPNFRR 360

Query: 361 TNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERD 420
           T SD+ ++ F  + SP +S +  + S  +P             SSSFNRIC  C+  E+D
Sbjct: 361 TESDISSTSFYGSSSPSHSALLRSSSVSSP-------------SSSFNRICSKCYINEKD 420

Query: 421 LVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
           L FGCGHQ CS CG+DL  CP CQT I TRI+L
Sbjct: 421 LAFGCGHQICSVCGEDLILCPICQTHITTRIRL 434

BLAST of MS010690 vs. NCBI nr
Match: XP_022950531.1 (E3 ubiquitin-protein ligase RGLG5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 595.1 bits (1533), Expect = 4.9e-166
Identity = 296/442 (66.97%), Postives = 334/442 (75.57%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MG K SK I CH H +G  + Q   CSC    P S ++A   R P+ + NGDDF+SLEQV
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPSTNSARSSRPPNPIINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           + AL +AGLESSNLI+GIDFT+SNEWTGKKSFMGRNLHDL G C NPYEQAISIIG+TL 
Sbjct: 61  SEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN IPCYGFGD TTGD+ VFSFH  D PCHGFEEVL CYRE  PHV LAGPTSF P
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRSVDT  GH SPQEQSTI+AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQMY+CDDQIPAR FDNFQFVNFT+IMS   P  +K+TEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YKAT+ LQLLGQKR T+G  PFP+P PPPI  IS N        P++RRT SD+ ++ F 
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRN------HFPNFRRTESDISSTSFY 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
            + SP +S +  + S  +P             S SFNRIC  C+  E+DL  GCGHQ CS
Sbjct: 361 GSSSPSHSALLRSSSVSSP-------------SLSFNRICSQCYINEKDLALGCGHQICS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKL 443
            CG+DL  CP CQT I TRI+L
Sbjct: 421 VCGEDLILCPICQTHITTRIRL 423

BLAST of MS010690 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.2e-127
Identity = 237/397 (59.70%), Postives = 278/397 (70.03%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  DD+ SLEQVT ALA+AGLESSNLI+GIDFTKSNEWTG +SF  ++LH +G   PN
Sbjct: 96  YSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSS-PN 155

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ EDR C+GFEEVLS Y+E 
Sbjct: 156 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEI 215

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ IV  S GQYH+L+IIADGQVTRSVDT  G  SPQEQ T+
Sbjct: 216 VPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTV 275

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+ASK PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IM+       KE
Sbjct: 276 DAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKE 335

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPI-ANISLNSSPSYRRSP 346
           TEFAL +LMEIP QYKATI L LLG++    G  P   PLPPP+    S  +SP   R P
Sbjct: 336 TEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLP 395

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++ +    PT  + +  SP                      PP + S+S N++CP+C S
Sbjct: 396 SFKPSVPPHPTEGYHVRSSP---------------------VPPPTSSASDNQLCPICLS 455

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +D+ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 456 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of MS010690 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 8.5e-121
Identity = 224/397 (56.42%), Postives = 273/397 (68.77%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y +  D++ S+++VT AL+ AGLESSNLI+GID TKSNEWTG +SF  ++LH + G  PN
Sbjct: 67  YSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKSLHFI-GTTPN 126

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PY+QAISIIG+TL  FDEDN IPCYGFGDATT DQ VFSF+  D  C+GFEEVL CYRE 
Sbjct: 127 PYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFEEVLMCYREI 186

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P ++L+GPTSF PII  A+ IV +S GQYH+LLIIADGQVTRSVDT  G FSPQEQ TI
Sbjct: 187 VPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGGFSPQEQQTI 246

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV AS+YPLSI+LVGVG+GPWD M + DD IPAR FDNFQFVNFT+IMS  I   RKE
Sbjct: 247 DAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMSKNIDPARKE 306

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPP-IANISLNSSPSYRRSP 346
            EFAL +LMEIP QYKAT+ L LLGQ+    G  P  + LPPP  A  S+ +SP   RS 
Sbjct: 307 AEFALSALMEIPSQYKATLELGLLGQR---TGHCPDRIALPPPTYATQSMRNSPRTSRST 366

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++                P ++ + +TP             P  + + S  + CPVC  
Sbjct: 367 SFQN--------------KPYDNGVSSTP-------------PSTTHNESQQQFCPVCLV 426

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +++ F CGHQ C+ CG+DL  CP C++ I  RIKL
Sbjct: 427 SAKNMAFNCGHQTCAGCGEDLHVCPICRSSISVRIKL 432

BLAST of MS010690 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 433.3 bits (1113), Expect = 3.2e-120
Identity = 230/396 (58.08%), Postives = 271/396 (68.43%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  D++ SL QV+ AL +AGLESSNLI+GIDFTKSNEWTG KSF  ++LH L  + PN
Sbjct: 130 YSKISDNYSSLLQVSEALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHL-SNTPN 189

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ E R C+GFEEVL+ YRE 
Sbjct: 190 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREI 249

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ +V  S GQYH+L+IIADGQVTRSVDT +G  SPQEQ T+
Sbjct: 250 VPQLKLAGPTSFAPIIEMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTV 309

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+AS  PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IMS      RKE
Sbjct: 310 DAIVKASTLPLSIVLVGVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKE 369

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPS 346
           TEFAL +LMEIPPQYKATI L LLG +    G  P  +PLPPP+ +    SS S  R P+
Sbjct: 370 TEFALSALMEIPPQYKATIELNLLGVR---NGNIPQRIPLPPPVQS---GSSFSSSRIPN 429

Query: 347 YRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSK 406
           +               PS P            P P  S +      S+   ++CP+C S 
Sbjct: 430 FE--------------PSVP------------PYPFESKQMS----SADDIQLCPICLSN 488

Query: 407 ERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
            +++ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 490 PKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKL 488

BLAST of MS010690 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.0e-107
Identity = 198/391 (50.64%), Postives = 256/391 (65.47%), Query Frame = 0

Query: 52  DDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQA 111
           D F SL+QV+ AL +AGLESSNLI+G+DFTKSNEWTGK SF G+ LH L G+  NPYE+A
Sbjct: 58  DRFSSLDQVSKALREAGLESSNLILGVDFTKSNEWTGKTSFDGKCLHAL-GETSNPYEKA 117

Query: 112 ISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVK 171
           I +IG+TL  FDEDN IPC+GFGD+TT D++VF FH ++ PCHGFEEVL+CY+  AP+++
Sbjct: 118 IFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKRIAPNLR 177

Query: 172 LAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVE 231
           L+GPTS+GP+I  A+ IV  ++GQ+H+L+I+ADGQVTR  D   G  S QE++TIDAIV 
Sbjct: 178 LSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTTIDAIVN 237

Query: 232 ASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFAL 291
           AS Y LSI+LVGVG+GPW+ M + DD+IP R+FDNFQFVNFT IM+   P   KET FAL
Sbjct: 238 ASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAKETAFAL 297

Query: 292 QSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTN 351
            +LMEIP QY+A I L+LLG   K  G     +P PPPI                     
Sbjct: 298 AALMEIPFQYQAAIELRLLG---KQTGLAKTIVPRPPPI--------------------- 357

Query: 352 SDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLV 411
              P +P      P N+ + +T S  +P  + S               CP+C +  +D+ 
Sbjct: 358 ---PYTP------PTNAELPSTASPASPEQTQS---------------CPICLTNRKDVA 399

Query: 412 FGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
           F CGH  C DCG  +  CP C+ +I  R+KL
Sbjct: 418 FSCGHMTCGDCGSKISNCPICRVRITNRLKL 399

BLAST of MS010690 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.5e-101
Identity = 192/400 (48.00%), Postives = 250/400 (62.50%), Query Frame = 0

Query: 43  RSPDYLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGG 102
           + P Y+   D F SL+QV  +L +AGLESSNLI+GIDFTKSNEWTG+ SF  ++LH + G
Sbjct: 9   QQPSYI--ADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAI-G 68

Query: 103 DCPNPYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSC 162
              NPYE+AISIIGRTL  FDED+ IPC+GFGD TT DQ VFSF+ E++ C G E  +  
Sbjct: 69  KRQNPYEKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKR 128

Query: 163 YRERAPHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQE 222
           YRE  PH+KL+GPTSF P+I  AI IV  ++ QYH+L+IIADGQVTR+ D P G  SPQE
Sbjct: 129 YREIVPHLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQE 188

Query: 223 QSTIDAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPT 282
           ++T+++I+ AS YPLSI+LVGVG+GPWD M + DD IP R+FDNFQFVNFT IMS     
Sbjct: 189 EATMNSIMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDA 248

Query: 283 PRKETEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYR 342
            +KE  FAL +LMEIP QYKAT+SL     ++          PLPPP   I  +++    
Sbjct: 249 AKKEAAFALAALMEIPFQYKATLSL----NRKPVRSSHQHHKPLPPPPEVIERDNA---- 308

Query: 343 RSPSYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPV 402
                                      + + P+    +   S R  P ++      +CP+
Sbjct: 309 ---------------------------VRSVPNQMTETAEKSDRLAPSTVP-----VCPI 365

Query: 403 CFSKERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
           C +  +D+ F CGH  C +CG  +  CP C+  I TRI+L
Sbjct: 369 CLTNPKDMAFSCGHTTCKECGVVITTCPLCRQPITTRIRL 365

BLAST of MS010690 vs. ExPASy TrEMBL
Match: A0A6J1BUE9 (E3 ubiquitin-protein ligase RGLG2-like OS=Momordica charantia OX=3673 GN=LOC111005456 PE=4 SV=1)

HSP 1 Score: 887.9 bits (2293), Expect = 1.8e-254
Identity = 427/432 (98.84%), Postives = 428/432 (99.07%), Query Frame = 0

Query: 13  FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS 72
           FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS
Sbjct: 1   FHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQVTGALAQAGLESS 60

Query: 73  NLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCYG 132
           NLIIGIDFTKSNEWTGKKSF GRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCY 
Sbjct: 61  NLIIGIDFTKSNEWTGKKSFTGRNLHDLGGDCPNPYEQAISIIGRTLRQFDEDNRIPCYS 120

Query: 133 FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS 192
           FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS
Sbjct: 121 FGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGPIIRNAIRIVNDS 180

Query: 193 DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSIILVGVGEGPWDQM 252
           DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYP SIILVGVG+GPWDQM
Sbjct: 181 DGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPSSIILVGVGDGPWDQM 240

Query: 253 YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ 312
           YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ
Sbjct: 241 YECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQYKATISLQLLGQ 300

Query: 313 KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT 372
           KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT
Sbjct: 301 KRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFGLAPSPPNSNIHT 360

Query: 373 TPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC 432
            PSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC
Sbjct: 361 PPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACSDCGKDLFFCPSC 420

Query: 433 QTQIETRIKLIN 445
           QTQIETRIKLIN
Sbjct: 421 QTQIETRIKLIN 432

BLAST of MS010690 vs. ExPASy TrEMBL
Match: A0A5D3CLM2 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00410 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.5e-168
Identity = 303/444 (68.24%), Postives = 336/444 (75.68%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MGGKPSK I  H H H   + Q S CSC    P S S+   F+ P +  NGDDF+SLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           T AL +AGLESSNLIIGIDFTKSNEWTG KSFMGRNLHDL G   NPYEQAISIIGRTL 
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN+IPCYGFGD TTGD++VFSFH  DRPCHGFEEVL CYRE  P V LAGPTSF P
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR++DT  GHFSPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQM ECDD+IPAR+FDNFQFVNFT IMS      +KETEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YK T+ LQLLGQK++T+   PFP+P PPPI NI  N  P     PSYRRT+SD+ +S  G
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYP-----PSYRRTDSDISSSFSG 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
                            +P+ S   RS   S + SFNRICP C+  E++L FGCGHQ CS
Sbjct: 361 ---------------STSPAHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKLIN 445
           DCGKDL FCP+CQTQI   IKL N
Sbjct: 421 DCGKDLLFCPTCQTQITKSIKLRN 424

BLAST of MS010690 vs. ExPASy TrEMBL
Match: A0A1S3B2Y1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103485213 PE=4 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.5e-168
Identity = 303/444 (68.24%), Postives = 336/444 (75.68%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MGGKPSK I  H H H   + Q S CSC    P S S+   F+ P +  NGDDF+SLEQV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           T AL +AGLESSNLIIGIDFTKSNEWTG KSFMGRNLHDL G   NPYEQAISIIGRTL 
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN+IPCYGFGD TTGD++VFSFH  DRPCHGFEEVL CYRE  P V LAGPTSF P
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR++DT  GHFSPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQM ECDD+IPAR+FDNFQFVNFT IMS      +KETEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YK T+ LQLLGQK++T+   PFP+P PPPI NI  N  P     PSYRRT+SD+ +S  G
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYP-----PSYRRTDSDISSSFSG 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
                            +P+ S   RS   S + SFNRICP C+  E++L FGCGHQ CS
Sbjct: 361 ---------------STSPAHSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKLIN 445
           DCGKDL FCP+CQTQI   IKL N
Sbjct: 421 DCGKDLLFCPTCQTQITKSIKLRN 424

BLAST of MS010690 vs. ExPASy TrEMBL
Match: A0A6J1GF30 (E3 ubiquitin-protein ligase RGLG5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453613 PE=4 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 2.4e-166
Identity = 296/442 (66.97%), Postives = 334/442 (75.57%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MG K SK I CH H +G  + Q   CSC    P S ++A   R P+ + NGDDF+SLEQV
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPSTNSARSSRPPNPIINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           + AL +AGLESSNLI+GIDFT+SNEWTGKKSFMGRNLHDL G C NPYEQAISIIG+TL 
Sbjct: 61  SEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN IPCYGFGD TTGD+ VFSFH  D PCHGFEEVL CYRE  PHV LAGPTSF P
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRSVDT  GH SPQEQSTI+AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQMY+CDDQIPAR FDNFQFVNFT+IMS   P  +K+TEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YKAT+ LQLLGQKR T+G  PFP+P PPPI  IS N        P++RRT SD+ ++ F 
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRN------HFPNFRRTESDISSTSFY 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
            + SP +S +  + S  +P             S SFNRIC  C+  E+DL  GCGHQ CS
Sbjct: 361 GSSSPSHSALLRSSSVSSP-------------SLSFNRICSQCYINEKDLALGCGHQICS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKL 443
            CG+DL  CP CQT I TRI+L
Sbjct: 421 VCGEDLILCPICQTHITTRIRL 423

BLAST of MS010690 vs. ExPASy TrEMBL
Match: A0A6J1INP4 (E3 ubiquitin-protein ligase RGLG5-like OS=Cucurbita maxima OX=3661 GN=LOC111478078 PE=4 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 3.1e-166
Identity = 297/442 (67.19%), Postives = 333/442 (75.34%), Query Frame = 0

Query: 1   MGGKPSKQIECHFHFHGADQGQSSNCSCVCHSPSSISTASIFRSPDYLKNGDDFDSLEQV 60
           MG K SK I CH HF+G  + Q   CSC    P S ++A   R P  + NGDDF+SLEQV
Sbjct: 1   MGAKSSKNIHCHVHFYGDHRNQPLRCSCGHSLPPSTNSAPSSRPPSPIINGDDFESLEQV 60

Query: 61  TGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPNPYEQAISIIGRTLR 120
           + AL  AGLESSNLI+GIDFTKSNEWTGKKSFMGRNLHDL G C NPYEQAISIIG+TL 
Sbjct: 61  SEALENAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 QFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRERAPHVKLAGPTSFGP 180
           +FD+DN IPCYGFGD TTGD++VFSFH  D PCHGFEEVL CYRE  PHV LAGPTSF P
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRSVDT  GH S QEQSTI+AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSSQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKETEFALQSLMEIPPQ 300
           LVGVG+GPWDQMY+CDDQIPAR FDNFQFVNFT+IMS   P  +K+TEFALQ LMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARNFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YKATISLQLLGQKRKTEGRFPFPMPLPPPIANISLNSSPSYRRSPSYRRTNSDMPTSPFG 360
           YKAT+ LQLLGQKR T+G  PFP+P PPPI  IS N        P++RRT SD+ ++ F 
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRN------HFPNFRRTESDISSTSFY 360

Query: 361 LAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFSKERDLVFGCGHQACS 420
            + SP +S +  + S  +P             SSSFNRIC  C+  E+DL  GCGHQ CS
Sbjct: 361 GSSSPSHSALLRSSSVSSP-------------SSSFNRICSQCYINEKDLALGCGHQICS 420

Query: 421 DCGKDLFFCPSCQTQIETRIKL 443
            CG+DL  CP CQT I  RI+L
Sbjct: 421 ACGEDLIACPICQTHITIRIRL 423

BLAST of MS010690 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 458.0 bits (1177), Expect = 8.7e-129
Identity = 237/397 (59.70%), Postives = 278/397 (70.03%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  DD+ SLEQVT ALA+AGLESSNLI+GIDFTKSNEWTG +SF  ++LH +G   PN
Sbjct: 96  YSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSS-PN 155

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ EDR C+GFEEVLS Y+E 
Sbjct: 156 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEI 215

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ IV  S GQYH+L+IIADGQVTRSVDT  G  SPQEQ T+
Sbjct: 216 VPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTV 275

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+ASK PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IM+       KE
Sbjct: 276 DAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKE 335

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPI-ANISLNSSPSYRRSP 346
           TEFAL +LMEIP QYKATI L LLG++    G  P   PLPPP+    S  +SP   R P
Sbjct: 336 TEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLP 395

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++ +    PT  + +  SP                      PP + S+S N++CP+C S
Sbjct: 396 SFKPSVPPHPTEGYHVRSSP---------------------VPPPTSSASDNQLCPICLS 455

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +D+ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 456 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of MS010690 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 458.0 bits (1177), Expect = 8.7e-129
Identity = 237/397 (59.70%), Postives = 278/397 (70.03%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  DD+ SLEQVT ALA+AGLESSNLI+GIDFTKSNEWTG +SF  ++LH +G   PN
Sbjct: 96  YSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSS-PN 155

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ EDR C+GFEEVLS Y+E 
Sbjct: 156 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEI 215

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ IV  S GQYH+L+IIADGQVTRSVDT  G  SPQEQ T+
Sbjct: 216 VPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTV 275

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+ASK PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IM+       KE
Sbjct: 276 DAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKE 335

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPI-ANISLNSSPSYRRSP 346
           TEFAL +LMEIP QYKATI L LLG++    G  P   PLPPP+    S  +SP   R P
Sbjct: 336 TEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLP 395

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++ +    PT  + +  SP                      PP + S+S N++CP+C S
Sbjct: 396 SFKPSVPPHPTEGYHVRSSP---------------------VPPPTSSASDNQLCPICLS 455

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +D+ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 456 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of MS010690 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 458.0 bits (1177), Expect = 8.7e-129
Identity = 237/397 (59.70%), Postives = 278/397 (70.03%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  DD+ SLEQVT ALA+AGLESSNLI+GIDFTKSNEWTG +SF  ++LH +G   PN
Sbjct: 96  YSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSS-PN 155

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ EDR C+GFEEVLS Y+E 
Sbjct: 156 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEI 215

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ IV  S GQYH+L+IIADGQVTRSVDT  G  SPQEQ T+
Sbjct: 216 VPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTV 275

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+ASK PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IM+       KE
Sbjct: 276 DAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKE 335

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPI-ANISLNSSPSYRRSP 346
           TEFAL +LMEIP QYKATI L LLG++    G  P   PLPPP+    S  +SP   R P
Sbjct: 336 TEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLP 395

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++ +    PT  + +  SP                      PP + S+S N++CP+C S
Sbjct: 396 SFKPSVPPHPTEGYHVRSSP---------------------VPPPTSSASDNQLCPICLS 455

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +D+ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 456 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of MS010690 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 458.0 bits (1177), Expect = 8.7e-129
Identity = 237/397 (59.70%), Postives = 278/397 (70.03%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y K  DD+ SLEQVT ALA+AGLESSNLI+GIDFTKSNEWTG +SF  ++LH +G   PN
Sbjct: 96  YSKISDDYSSLEQVTEALARAGLESSNLIVGIDFTKSNEWTGARSFNRKSLHFIGSS-PN 155

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PYEQAI+IIGRTL  FDEDN IPCYGFGDA+T DQ VFSF+ EDR C+GFEEVLS Y+E 
Sbjct: 156 PYEQAITIIGRTLAAFDEDNLIPCYGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEI 215

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P +KLAGPTSF PII  A+ IV  S GQYH+L+IIADGQVTRSVDT  G  SPQEQ T+
Sbjct: 216 VPQLKLAGPTSFAPIIDMAMTIVEQSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTV 275

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV+ASK PLSI+LVGVG+GPWD M E DD IPAR FDNFQFVNFT IM+       KE
Sbjct: 276 DAIVQASKLPLSIVLVGVGDGPWDMMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKE 335

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPPI-ANISLNSSPSYRRSP 346
           TEFAL +LMEIP QYKATI L LLG++    G  P   PLPPP+    S  +SP   R P
Sbjct: 336 TEFALSALMEIPQQYKATIELNLLGRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLP 395

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++ +    PT  + +  SP                      PP + S+S N++CP+C S
Sbjct: 396 SFKPSVPPHPTEGYHVRSSP---------------------VPPPTSSASDNQLCPICLS 455

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +D+ FGCGHQ C +CG DL  CP C+  I+TRIKL
Sbjct: 456 NPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of MS010690 vs. TAIR 10
Match: AT1G67800.1 (Copine (Calcium-dependent phospholipid-binding protein) family )

HSP 1 Score: 435.3 bits (1118), Expect = 6.0e-122
Identity = 224/397 (56.42%), Postives = 273/397 (68.77%), Query Frame = 0

Query: 47  YLKNGDDFDSLEQVTGALAQAGLESSNLIIGIDFTKSNEWTGKKSFMGRNLHDLGGDCPN 106
           Y +  D++ S+++VT AL+ AGLESSNLI+GID TKSNEWTG +SF  ++LH + G  PN
Sbjct: 67  YSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKSLHFI-GTTPN 126

Query: 107 PYEQAISIIGRTLRQFDEDNRIPCYGFGDATTGDQQVFSFHLEDRPCHGFEEVLSCYRER 166
           PY+QAISIIG+TL  FDEDN IPCYGFGDATT DQ VFSF+  D  C+GFEEVL CYRE 
Sbjct: 127 PYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFEEVLMCYREI 186

Query: 167 APHVKLAGPTSFGPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTPYGHFSPQEQSTI 226
            P ++L+GPTSF PII  A+ IV +S GQYH+LLIIADGQVTRSVDT  G FSPQEQ TI
Sbjct: 187 VPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGGFSPQEQQTI 246

Query: 227 DAIVEASKYPLSIILVGVGEGPWDQMYECDDQIPARKFDNFQFVNFTNIMSMAIPTPRKE 286
           DAIV AS+YPLSI+LVGVG+GPWD M + DD IPAR FDNFQFVNFT+IMS  I   RKE
Sbjct: 247 DAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMSKNIDPARKE 306

Query: 287 TEFALQSLMEIPPQYKATISLQLLGQKRKTEGRFPFPMPLPPP-IANISLNSSPSYRRSP 346
            EFAL +LMEIP QYKAT+ L LLGQ+    G  P  + LPPP  A  S+ +SP   RS 
Sbjct: 307 AEFALSALMEIPSQYKATLELGLLGQR---TGHCPDRIALPPPTYATQSMRNSPRTSRST 366

Query: 347 SYRRTNSDMPTSPFGLAPSPPNSNIHTTPSGFAPSPSPSGRSPPLSLSSSFNRICPVCFS 406
           S++                P ++ + +TP             P  + + S  + CPVC  
Sbjct: 367 SFQN--------------KPYDNGVSSTP-------------PSTTHNESQQQFCPVCLV 426

Query: 407 KERDLVFGCGHQACSDCGKDLFFCPSCQTQIETRIKL 443
             +++ F CGHQ C+ CG+DL  CP C++ I  RIKL
Sbjct: 427 SAKNMAFNCGHQTCAGCGEDLHVCPICRSSISVRIKL 432