MS010689 (gene) Bitter gourd (TR) v1

Overview


Name	MS010689
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	ATP-dependent 6-phosphofructokinase
Location	scaffold35: 1422375 .. 1424540 (+)
RNA-Seq Expression	MS010689
Synteny	MS010689

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATAATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGTGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTTTAATTTGTTTCTTTTTCTCTCTGTTTCCACTTTCGATTGGTTCGTTTTTCTTTTTATTTATCCTCTCGATTAGATGGTGTAGAAATGAAGGAAAATTTTAGAAATAAAATACTAGAGAAAAGAAAAATCATAGTGTGAAGGTATGGAGTAGTACTCCTTTTTAGCAATAGAATAAAATTTGATAACTATGGAGCAAGATGGAGCAAGGAAAACAAAATAGGATAAATAGTTGTCAAATTTTTTTAATCATGGCGAGTGAAAAAATACATATTCACCAGTAAAGACCTAAGTTTTATCTTTATTATTTTGTTCTTGAAATACATGCGAAAATATATATTTTGTAAAATTTTATTTTAACTTAGATGAAAATAATGTGATTTTATCGCGATAACACATTTTTGGACTTATTTCAAGAAGAAAAACACACACGTTTTTTTTTTCCTGAATNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAAATTGTAATTTTAGCATTATATAATTCTGTTTGAGACTTTGAGTGAGTAAGTAATTACGAATTGTACTAAAAAAAAAGGAAATTAGGAATTAAGAAAAGAAGAAAACAAAACAAGAAGTACTATATTTTTTAAAAGAAGTCTATATTTTAAAAACAATTTTCTGGAGAAAAATCTATAATATTTGATCACCATTCACTGCACGTCATGTCCAATAATTTGCAGTTTTTGGTCTTAGGAGTTTGATTGAATTGGGGTTGTTTCAATGGCGATTTGTTGCGAATGAATAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTAGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAATTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGC

mRNA sequence

ATAATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGTGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTAGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAATTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGC

Coding sequence (CDS)

ATAATGGCCGCCGGAACCACCGATCAACAGCGCTCCATCGGCGTCGAAAAGCTCCCCCACCTCACGGACTATTTCCCGGACCTCCAGACCCACACAAACCCTCTCCAGAGCAACCAATTCTTCCACCCATCCGACGGCTTCTACGTCACGCCCACCGACGTCGTCCTCCGCCACACCGCCTTCGACCTCGCCGCCAACTTTCCGGCGACGGCGGCGGCCCCACTCCAGGCGTACCATCGCGCCGGTCCGCGCCGGTCCATCTATTTCGAACCGGGAAGTGTCCGGGCGGCGATCGTCACGTGTGGGGGGCTGTGCCCGGGGATGAACACCGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGGTGTACGGCGTGCGGCGGATCTGTGGGATCGTGGCCGGATACAGAGGGTTTTATTCCGACAACGCGGTGGAGCTGAACCCGAAGCTGGTCCATAACTGGCATAAGAGAGGCGGCACCGCCCTCGAAACCTCCAGAGGGGGATTCGATCTCCACAAAATCGTTGATGCGATACAACATCAGGGTTTCAATCAGGTCTACATTATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGACGAAATATGTCGTCGGAGACTTCAAGTCGCCGTCACAGGAATTCCGAAAACCGTCGACAACGACGTCGGAATCATCGATCAATCATTTGGATTTCAAACGGCCGTCGAAATGGCGCAGCAGGCAATCAGTGCCGCCCATGTGGAGGCCGAGAGCGCGGTGAACGGCATAGGTCTGGTCAAATTGATGGGCCGAAGCACCGGCCACATCGCTCTTCACGCCACACTGAGCAGCCGAGACGTCGATTGCTGCTTAATACCTGAAATGGCCTTCTACTTGGAAGGCAGCGGAGGGTTGTTTACGTTTCTCGATCAGAAACTGAAGGAGAATGGCCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGGCAAGATTTGATACCTAGAACTGAGGCTCAAAAAGAAGAGAGGGACGAATCTGGAAATCCCGTCTTCTTAGACGTGGGCGGATGGTTGAAGACGGAGCTCAGGAATTGGTGGGCGAGAACGCACCCTGGTGAGTTGTTTACAGTAAAATATATTGATCCTACTTATATGATCCGTGCGGTGCCTGCAAATGCTACGGATAATTCCTACTGTTCCTTGCTGGCTCATTCGGCCATCCATGGCGTAATGGCTGGTTATACTGGATTTGTCCCTGGCCCTATTAATAGACACTATTCATATATTCCATTGAAAGAGGTGGCAGAAGCAAAGAATGAAGTTAATACGAGAGATCATAAATGGGCATGGGTTAGATCCGTCACCAATCAGCCTGATTTTGTCAGAAGC

Protein sequence

IMAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS

Homology

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BLAST of MS010689 vs. NCBI nr
Match: XP_022132614.1 (ATP-dependent 6-phosphofructokinase 2 [Momordica charantia])

HSP 1 Score: 948.7 bits (2451), Expect = 1.8e-272
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0


Query: 2   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 61
           MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF
Sbjct: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60

Query: 62  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 121
           DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 122 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 181
           VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ
Sbjct: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180

Query: 182 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 241
           GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240

Query: 242 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 301
           QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL
Sbjct: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300

Query: 302 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 361
           FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW
Sbjct: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360

Query: 362 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 421
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420

Query: 422 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 457

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BLAST of MS010689 vs. NCBI nr
Match: XP_038881531.1 (ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 805.8 bits (2080), Expect = 1.9e-229
Identity = 393/456 (86.18%), Postives = 413/456 (90.57%), Query Frame = 0


Query: 4   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 63
           A  +DQ   I V  LPHLTD FPDL+T TNPLQ+NQFFHP DGFY + TDVVLR  AFDL
Sbjct: 4   AKISDQHPPIAVADLPHLTDDFPDLETRTNPLQTNQFFHPIDGFYASSTDVVLRRIAFDL 63

Query: 64  AANFPATAAAPLQ-AYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 123
                +T  +P   AYHRAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 64  -----STKESPAHVAYHRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 123

Query: 124 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 183
           YGVRRI GI+AGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVD IQH G
Sbjct: 124 YGVRRIYGILAGYRGFYSTEPVELNLKLVDNWHKRGGTALRTSRGGFDLRKIVDTIQHHG 183

Query: 184 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 243
           FNQVYIIGGDGTMRGAVKIFDEI RR LQVAVTGIPKTVDND+GI+D+SFGFQTAVEMAQ
Sbjct: 184 FNQVYIIGGDGTMRGAVKIFDEIRRRNLQVAVTGIPKTVDNDIGIVDRSFGFQTAVEMAQ 243

Query: 244 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 303
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEG GGLF
Sbjct: 244 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGDGGLF 303

Query: 304 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 363
            FL+++LKENGHAVLVVAEGAGQ +IPRTEA+KEERDESGNPVFLDVGGWLKTEL+NWWA
Sbjct: 304 PFLERRLKENGHAVLVVAEGAGQSMIPRTEAEKEERDESGNPVFLDVGGWLKTELKNWWA 363

Query: 364 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 423
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 364 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 423

Query: 424 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 424 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 454

[Close]

BLAST of MS010689 vs. NCBI nr
Match: XP_022950529.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata])

HSP 1 Score: 794.3 bits (2050), Expect = 5.7e-226
Identity = 386/456 (84.65%), Postives = 413/456 (90.57%), Query Frame = 0


Query: 3   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 62
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 5   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 64

Query: 63  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 122
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 65  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 124

Query: 123 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 182
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 125 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 184

Query: 183 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 242
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 185 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 244

Query: 243 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 302
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 245 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 304

Query: 303 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 362
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 305 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 364

Query: 363 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 422
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 365 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 424

Query: 423 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV +
Sbjct: 425 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

[Close]

BLAST of MS010689 vs. NCBI nr
Match: XP_008440953.1 (PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo])

HSP 1 Score: 793.9 bits (2049), Expect = 7.4e-226
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0


Query: 4   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 63
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 64  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 123
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 124 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 183
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 184 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 243
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 244 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 303
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 304 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 363
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 423
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

[Close]

BLAST of MS010689 vs. NCBI nr
Match: KAG6603845.1 (ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034027.1 ATP-dependent 6-phosphofructokinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 793.5 bits (2048), Expect = 9.7e-226
Identity = 385/456 (84.43%), Postives = 413/456 (90.57%), Query Frame = 0


Query: 3   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 62
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 6   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 65

Query: 63  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 122
           L+   P+       AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 66  LSRK-PSPGHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 125

Query: 123 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 182
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 126 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 185

Query: 183 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 242
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 186 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 245

Query: 243 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 302
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 246 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 305

Query: 303 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 362
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 306 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 365

Query: 363 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 422
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 366 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 425

Query: 423 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV++
Sbjct: 426 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 457

[Close]

BLAST of MS010689 vs. ExPASy Swiss-Prot
Match: Q9FIK0 (ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE=1 SV=1)

HSP 1 Score: 699.1 bits (1803), Expect = 3.3e-200
Identity = 336/441 (76.19%), Postives = 379/441 (85.94%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           + KLP L+     L+   NPL+ N +FHPS+GFY+TP+DV+L   A+D +A+  +     
Sbjct: 7   IRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHSQSRV--- 66

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
             AYHRAGPRR I +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWE+YGVR I GI AG
Sbjct: 67  --AYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAG 126

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFYS  AV+L+PK VH+WHK+GGT L TSRGGF L KIVDAI   G+NQVYIIGGDGT
Sbjct: 127 YRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGT 186

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
           MRGAV+IF EI  R+L+V +T IPKTVDNDVGIID+SFGFQTAVEMAQ+AISAAHVEAES
Sbjct: 187 MRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAES 246

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FL+++LKE GH
Sbjct: 247 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGH 306

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
           AVLVVAEGAGQ++IPR E+QK+ERDESGN VFLDVG W K+ L+ WW R HP ELFTVKY
Sbjct: 307 AVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKY 366

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRAVPANATDN YC+LLAHSAIHGVMAGYTGFVPGPIN +Y+YIPL+EVA+ KN
Sbjct: 367 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKN 426

Query: 435 EVNTRDHKWAWVRSVTNQPDF 456
           +VNTRDHKWAWVRSVTNQPDF
Sbjct: 427 QVNTRDHKWAWVRSVTNQPDF 438

[Close]

BLAST of MS010689 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.8e-141
Identity = 241/442 (54.52%), Postives = 316/442 (71.49%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTN 490

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 504

[Close]

BLAST of MS010689 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 4.2e-139
Identity = 247/442 (55.88%), Postives = 317/442 (71.72%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHL+DY  DL T+ NPLQSN  +     ++V   D V       +  + P      
Sbjct: 21  LEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIV--VHKDSPRGT--- 80

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR+ +YF+P  VRA IVTCGGLCPG+NTVIRE+V GL  +YGV  + G+  G
Sbjct: 81  --HFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCG 140

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           +RGFYS N V L PK V + HKRGGT L TSRGG D  KIVD IQ +  NQVYIIGGDGT
Sbjct: 141 FRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGT 200

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            +GA  I+ EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEA S
Sbjct: 201 QKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATS 260

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH
Sbjct: 261 VENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGH 320

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQDL+  +    E++D SGN +  DVG W+  +++ ++A+ +  ++ T+KY
Sbjct: 321 MVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKY 380

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRA+PANA+DN Y +LLA SA+HG MAGYTGFV G +N  ++YIP   + E +N
Sbjct: 381 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 440

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
           +V   D  WA + S TNQP F+
Sbjct: 441 KVVITDRMWARMLSSTNQPSFM 451

[Close]

BLAST of MS010689 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 5.5e-139
Identity = 247/442 (55.88%), Postives = 316/442 (71.49%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           ++ +PHL DY PDL T+ NPLQ N  +     ++V   D V       +  + P      
Sbjct: 20  LQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVV--VHKDGPRGV--- 79

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGG 139

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFY+ N + LN K+V++ HKRGGT + TSRGG D +KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGT 199

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            RGA  IF+EI RRRL+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NGIG VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F++++LK++GH
Sbjct: 260 NENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGH 319

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQDL+ ++  +    D SGN +  DVG WL   +++ + +     +  +KY
Sbjct: 320 MVIVLAEGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKY 379

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG+  G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQN 439

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + S TNQP F+
Sbjct: 440 NVVITDRMWARLLSSTNQPSFL 451

[Close]

BLAST of MS010689 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 9.3e-139
Identity = 248/442 (56.11%), Postives = 315/442 (71.27%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHL+DY P L T+ NPLQ N  +     ++V   D V +     +  + P      
Sbjct: 20  LEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIV--VHKDGPRGI--- 79

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGG 139

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFY+ N V L+ K+V++ HKRGGT L TSRGG D  KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGT 199

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            RGA  IF+EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGLF +++++LKE+GH
Sbjct: 260 IENGIGVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGH 319

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            VLV+AEGAGQDL+ ++      +D SGN +  DVG WL   +++ +       +  +KY
Sbjct: 320 MVLVIAEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKY 379

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG++ G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQN 439

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + S TNQP F+
Sbjct: 440 HVVITDRMWARLLSSTNQPSFL 452

[Close]

BLAST of MS010689 vs. ExPASy TrEMBL
Match: A0A6J1BTK2 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111005433 PE=3 SV=1)

HSP 1 Score: 948.7 bits (2451), Expect = 8.7e-273
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0


Query: 2   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 61
           MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF
Sbjct: 1   MAAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAF 60

Query: 62  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 121
           DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  DLAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 122 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 181
           VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ
Sbjct: 121 VYGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQ 180

Query: 182 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 241
           GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMA 240

Query: 242 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 301
           QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL
Sbjct: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGL 300

Query: 302 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 361
           FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW
Sbjct: 301 FTFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWW 360

Query: 362 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 421
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHY 420

Query: 422 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS
Sbjct: 421 SYIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 457

[Close]

BLAST of MS010689 vs. ExPASy TrEMBL
Match: A0A6J1GG28 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111453612 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.7e-226
Identity = 386/456 (84.65%), Postives = 413/456 (90.57%), Query Frame = 0


Query: 3   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 62
           AA   D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 5   AAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 64

Query: 63  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 122
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 65  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 124

Query: 123 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 182
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 125 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 184

Query: 183 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 242
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 185 FNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 244

Query: 243 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 302
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 245 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 304

Query: 303 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 362
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 305 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 364

Query: 363 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 422
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 365 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 424

Query: 423 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           YIPLK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV +
Sbjct: 425 YIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVET 456

[Close]

BLAST of MS010689 vs. ExPASy TrEMBL
Match: A0A1S3B325 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.6e-226
Identity = 388/455 (85.27%), Postives = 413/455 (90.77%), Query Frame = 0


Query: 4   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 63
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 64  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 123
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 124 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 183
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 184 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 243
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 244 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 303
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 304 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 363
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 423
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

[Close]

BLAST of MS010689 vs. ExPASy TrEMBL
Match: A0A6J1ISR0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.7e-226
Identity = 385/456 (84.43%), Postives = 414/456 (90.79%), Query Frame = 0


Query: 3   AAGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFD 62
           AA  +D+   I V  LPHLTD+FP LQ+ TNPLQ+NQFFHP DGFY T TDVVLR  AFD
Sbjct: 7   AAEISDRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFD 66

Query: 63  LAANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEV 122
           L+   P+ A     AY+RAGPRRS+YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+
Sbjct: 67  LSRK-PSPAHV---AYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWEL 126

Query: 123 YGVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQG 182
           YGVR+I GIVAGYRGFYS   VEL+ KLV +WHKRGGTAL TSRGGFDL +IVDAIQ +G
Sbjct: 127 YGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRG 186

Query: 183 FNQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQ 242
           FNQVYIIGGDGTMRGAVKIF+E+CRR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQ
Sbjct: 187 FNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQ 246

Query: 243 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLF 302
           QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF
Sbjct: 247 QAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLF 306

Query: 303 TFLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWA 362
            FL+++LKENGHAVLVVAEGAGQD IPRTEAQKEERDESGNPVFLDVGGWLKTEL++WWA
Sbjct: 307 PFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWA 366

Query: 363 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYS 422
           RTHPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YS
Sbjct: 367 RTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYS 426

Query: 423 YIPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           YIPLK+VAEAKNEVNT+DHKWAWVRSVTNQPDFV +
Sbjct: 427 YIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFVET 458

[Close]

BLAST of MS010689 vs. ExPASy TrEMBL
Match: A0A5D3CPV9 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 792.0 bits (2044), Expect = 1.4e-225
Identity = 387/455 (85.05%), Postives = 412/455 (90.55%), Query Frame = 0


Query: 4   AGTTDQQRSIGVEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDL 63
           A  +DQ   I V  LPHLTD FP+L+T TNPLQ+NQFFHP DGFY T TD+VLR  AFDL
Sbjct: 4   AKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAFDL 63

Query: 64  AANFPATAAAPLQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVY 123
           + N   T+ A + AY RAGPRR++YFEP SVRAAIVTCGGLCPGMNTVIRELVVGLWE+Y
Sbjct: 64  SGN---TSPAHV-AYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELY 123

Query: 124 GVRRICGIVAGYRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGF 183
           GVRRI GIVAGYRGFYS   VELN KLV NWHKRGGTAL TSRGGFDL KIVDAI++ GF
Sbjct: 124 GVRRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGF 183

Query: 184 NQVYIIGGDGTMRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQ 243
           NQVYI GGDGTMRGAVKIF+EI RR LQVAVTGIPKTVDND+GIID+SFGFQTAVEMAQQ
Sbjct: 184 NQVYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQ 243

Query: 244 AISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFT 303
           AI+AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLF 
Sbjct: 244 AINAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFP 303

Query: 304 FLDQKLKENGHAVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWAR 363
           FL+Q+LKENGHAVLVVAEGAGQ +IPRTEAQKEERDESGNPVFLDVGGWLKTEL+NWW R
Sbjct: 304 FLEQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWER 363

Query: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSY 423
           THPGELFTVKYIDPTYMIRAVPANATDNSYC+LLAHSAIHGVMAGYTGFV GPIN +YSY
Sbjct: 364 THPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSY 423

Query: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 459
           IPLKEVAEAKNEVNTRDHKWAWVRSV NQPDFV+S
Sbjct: 424 IPLKEVAEAKNEVNTRDHKWAWVRSVANQPDFVKS 454

[Close]

BLAST of MS010689 vs. TAIR 10
Match: AT5G47810.1 (phosphofructokinase 2 )

HSP 1 Score: 699.1 bits (1803), Expect = 2.3e-201
Identity = 336/441 (76.19%), Postives = 379/441 (85.94%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           + KLP L+     L+   NPL+ N +FHPS+GFY+TP+DV+L   A+D +A+  +     
Sbjct: 7   IRKLPSLS----GLRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAHSQSRV--- 66

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
             AYHRAGPRR I +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWE+YGVR I GI AG
Sbjct: 67  --AYHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAG 126

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFYS  AV+L+PK VH+WHK+GGT L TSRGGF L KIVDAI   G+NQVYIIGGDGT
Sbjct: 127 YRGFYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGT 186

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
           MRGAV+IF EI  R+L+V +T IPKTVDNDVGIID+SFGFQTAVEMAQ+AISAAHVEAES
Sbjct: 187 MRGAVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAES 246

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
           AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG GGLF FL+++LKE GH
Sbjct: 247 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGH 306

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
           AVLVVAEGAGQ++IPR E+QK+ERDESGN VFLDVG W K+ L+ WW R HP ELFTVKY
Sbjct: 307 AVLVVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKY 366

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRAVPANATDN YC+LLAHSAIHGVMAGYTGFVPGPIN +Y+YIPL+EVA+ KN
Sbjct: 367 IDPTYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKN 426

Query: 435 EVNTRDHKWAWVRSVTNQPDF 456
           +VNTRDHKWAWVRSVTNQPDF
Sbjct: 427 QVNTRDHKWAWVRSVTNQPDF 438

[Close]

BLAST of MS010689 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 502.3 bits (1292), Expect = 4.2e-142
Identity = 241/442 (54.52%), Postives = 316/442 (71.49%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+ +V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTN 490

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 504

[Close]

BLAST of MS010689 vs. TAIR 10
Match: AT5G61580.2 (phosphofructokinase 4 )

HSP 1 Score: 496.9 bits (1278), Expect = 1.7e-140
Identity = 241/442 (54.52%), Postives = 315/442 (71.27%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHLT + PDL ++ NPL+ +Q +      +V+  DVV ++      +         
Sbjct: 71  LEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGV----- 130

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR  +YF    V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  G
Sbjct: 131 --HFRRAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGG 190

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFYS N + L PK+V++ HKRGGT L+TSRGG D  KIVD IQ +G NQVYIIGG GT
Sbjct: 191 YRGFYSKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGT 250

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            +GA KI++E+ RR LQVAV+GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVE ES
Sbjct: 251 QKGAEKIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVES 310

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NG+G+VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F++++LKEN H
Sbjct: 311 VENGVGIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRH 370

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQD + ++    E +D SGN + LDVG WL  ++++ +       +  +KY
Sbjct: 371 MVIVIAEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKY 430

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRA+P+NA+DN YC+LLA SA+HG MAGY+GF  GP+N  ++YIP+  V E  N
Sbjct: 431 IDPTYMIRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPI-SVTEVTN 490

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + + TNQP F+
Sbjct: 491 TVKLTDRMWARLLASTNQPSFL 503

[Close]

BLAST of MS010689 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 496.1 bits (1276), Expect = 3.0e-140
Identity = 247/442 (55.88%), Postives = 317/442 (71.72%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           +E +PHL+DY  DL T+ NPLQSN  +     ++V   D V       +  + P      
Sbjct: 21  LEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDTVQEKIV--VHKDSPRGT--- 80

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR+ +YF+P  VRA IVTCGGLCPG+NTVIRE+V GL  +YGV  + G+  G
Sbjct: 81  --HFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCG 140

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           +RGFYS N V L PK V + HKRGGT L TSRGG D  KIVD IQ +  NQVYIIGGDGT
Sbjct: 141 FRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGT 200

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            +GA  I+ EI RR L+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEA S
Sbjct: 201 QKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATS 260

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NGIG+VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ ++L+ENGH
Sbjct: 261 VENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGH 320

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQDL+  +    E++D SGN +  DVG W+  +++ ++A+ +  ++ T+KY
Sbjct: 321 MVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKY 380

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRA+PANA+DN Y +LLA SA+HG MAGYTGFV G +N  ++YIP   + E +N
Sbjct: 381 IDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQN 440

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
           +V   D  WA + S TNQP F+
Sbjct: 441 KVVITDRMWARMLSSTNQPSFM 451

[Close]

BLAST of MS010689 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 495.7 bits (1275), Expect = 3.9e-140
Identity = 247/442 (55.88%), Postives = 316/442 (71.49%), Query Frame = 0


Query: 15  VEKLPHLTDYFPDLQTHTNPLQSNQFFHPSDGFYVTPTDVVLRHTAFDLAANFPATAAAP 74
           ++ +PHL DY PDL T+ NPLQ N  +     ++V   D V       +  + P      
Sbjct: 20  LQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVV--VHKDGPRGV--- 79

Query: 75  LQAYHRAGPRRSIYFEPGSVRAAIVTCGGLCPGMNTVIRELVVGLWEVYGVRRICGIVAG 134
              + RAGPR+ +YFE   V A IVTCGGLCPG+NTVIRE+V  L  +YGV+RI GI  G
Sbjct: 80  --HFRRAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGG 139

Query: 135 YRGFYSDNAVELNPKLVHNWHKRGGTALETSRGGFDLHKIVDAIQHQGFNQVYIIGGDGT 194
           YRGFY+ N + LN K+V++ HKRGGT + TSRGG D +KIVD+IQ +G NQVYIIGGDGT
Sbjct: 140 YRGFYAKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGT 199

Query: 195 MRGAVKIFDEICRRRLQVAVTGIPKTVDNDVGIIDQSFGFQTAVEMAQQAISAAHVEAES 254
            RGA  IF+EI RRRL+VAV GIPKT+DND+ +ID+SFGF TAVE AQ+AI+AAHVEAES
Sbjct: 200 QRGASVIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAES 259

Query: 255 AVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGSGGLFTFLDQKLKENGH 314
             NGIG VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG GGLF F++++LK++GH
Sbjct: 260 NENGIGFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGH 319

Query: 315 AVLVVAEGAGQDLIPRTEAQKEERDESGNPVFLDVGGWLKTELRNWWARTHPGELFTVKY 374
            V+V+AEGAGQDL+ ++  +    D SGN +  DVG WL   +++ + +     +  +KY
Sbjct: 320 MVIVLAEGAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKY 379

Query: 375 IDPTYMIRAVPANATDNSYCSLLAHSAIHGVMAGYTGFVPGPINRHYSYIPLKEVAEAKN 434
           IDPTYMIRAVP+NA+DN YC+LLA SA+HG MAGYTG+  G +N   +YIP   + E +N
Sbjct: 380 IDPTYMIRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQN 439

Query: 435 EVNTRDHKWAWVRSVTNQPDFV 457
            V   D  WA + S TNQP F+
Sbjct: 440 NVVITDRMWARLLSSTNQPSFL 451

The following BLAST results are available for this feature:

BLAST of MS010689 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132614.1	1.8e-272	100.00	ATP-dependent 6-phosphofructokinase 2 [Momordica charantia]	[more]
XP_038881531.1	1.9e-229	86.18	ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida]	[more]
XP_022950529.1	5.7e-226	84.65	ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata]	[more]
XP_008440953.1	7.4e-226	85.27	PREDICTED: ATP-dependent 6-phosphofructokinase 2 [Cucumis melo]	[more]
KAG6603845.1	9.7e-226	84.43	ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. so...	[more]

BLAST of MS010689 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FIK0	3.3e-200	76.19	ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE...	[more]
Q9FKG3	5.8e-141	54.52	ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=...	[more]
Q9M076	4.2e-139	55.88	ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE...	[more]
Q9C5J7	5.5e-139	55.88	ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE...	[more]
Q94AA4	9.3e-139	56.11	ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE...	[more]

BLAST of MS010689 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BTK2	8.7e-273	100.00	ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110054...	[more]
A0A6J1GG28	2.7e-226	84.65	ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145361...	[more]
A0A1S3B325	3.6e-226	85.27	ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3...	[more]
A0A6J1ISR0	4.7e-226	84.43	ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 ...	[more]
A0A5D3CPV9	1.4e-225	85.05	ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF...	[more]

BLAST of MS010689 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47810.1	2.3e-201	76.19	phosphofructokinase 2	[more]
AT5G61580.1	4.2e-142	54.52	phosphofructokinase 4	[more]
AT5G61580.2	1.7e-140	54.52	phosphofructokinase 4	[more]
AT4G32840.1	3.0e-140	55.88	phosphofructokinase 6	[more]
AT5G56630.1	3.9e-140	55.88	phosphofructokinase 7	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022953	ATP-dependent 6-phosphofructokinase	PRINTS	PR00476	PHFRCTKINASE	coord: 215..232 score: 35.61 coord: 311..323 score: 37.28 coord: 98..117 score: 45.13 coord: 254..270 score: 28.51 coord: 182..198 score: 43.89 coord: 233..251 score: 26.05
IPR000023	Phosphofructokinase domain	PFAM	PF00365	PFK	coord: 96..403 e-value: 2.3E-54 score: 184.6
None	No IPR available	GENE3D	3.40.50.450		coord: 90..279 e-value: 4.0E-55 score: 188.0
None	No IPR available	PANTHER	PTHR45770:SF9	ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 2	coord: 10..458
None	No IPR available	PANTHER	PTHR45770	FAMILY NOT NAMED	coord: 10..458
IPR012004	Pyrophosphate-dependent phosphofructokinase TP0108-type	PIRSF	PIRSF000534	ATP_PFK_TP0108	coord: 4..458 e-value: 3.6E-170 score: 564.2
IPR012004	Pyrophosphate-dependent phosphofructokinase TP0108-type	HAMAP	MF_01981	Phosphofructokinase_II_X	coord: 18..456 score: 51.375706
IPR035966	Phosphofructokinase superfamily	SUPERFAMILY	53784	Phosphofructokinase	coord: 22..455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010689.1	MS010689.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006002	fructose 6-phosphate metabolic process
biological_process	GO:0006096	glycolytic process
molecular_function	GO:0003872	6-phosphofructokinase activity
molecular_function	GO:0005524	ATP binding