MS010674 (gene) Bitter gourd (TR) v1

BLAST of MS010674 vs. NCBI nr
Match: XP_022133363.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Momordica charantia] >XP_022133365.1 serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Momordica charantia])

HSP 1 Score: 1838.2 bits (4760), Expect = 0.0e+00
Identity = 926/932 (99.36%), Postives = 926/932 (99.36%), Query Frame = 0

Query: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60
           MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL
Sbjct: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60

Query: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120
           KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF
Sbjct: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120

Query: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180
           FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL
Sbjct: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180

Query: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240
           IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG
Sbjct: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240

Query: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300
           SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS
Sbjct: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300

Query: 301 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP 360
           HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP
Sbjct: 301 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP 360

Query: 361 LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK 420
           LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK
Sbjct: 361 LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK 420

Query: 421 VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW 480
           VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW
Sbjct: 421 VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW 480

Query: 481 FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 540
           FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK
Sbjct: 481 FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 540

Query: 541 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDT 600
           EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDT
Sbjct: 541 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDT 600

Query: 601 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 660
           EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ
Sbjct: 601 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 660

Query: 661 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR 720
           NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTR
Sbjct: 661 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTR 720

Query: 721 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 780
           AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP
Sbjct: 721 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 780

Query: 781 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 840
           DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD
Sbjct: 781 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 840

Query: 841 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 900
           EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS
Sbjct: 841 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 900

Query: 901 RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
           RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE
Sbjct: 901 RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 927

BLAST of MS010674 vs. NCBI nr
Match: XP_022133366.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 [Momordica charantia])

HSP 1 Score: 1560.8 bits (4040), Expect = 0.0e+00
Identity = 788/794 (99.24%), Postives = 788/794 (99.24%), Query Frame = 0

Query: 139 MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH 198
           MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH
Sbjct: 11  MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH 70

Query: 199 SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL 258
           SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL
Sbjct: 71  SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL 130

Query: 259 CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI 318
           CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI
Sbjct: 131 CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI 190

Query: 319 GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI 378
           GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI
Sbjct: 191 GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI 250

Query: 379 NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI 438
           NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI
Sbjct: 251 NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI 310

Query: 439 LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK 498
           LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK
Sbjct: 311 LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK 370

Query: 499 LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY 558
           LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY
Sbjct: 371 LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY 430

Query: 559 GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMP 618
           GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMP
Sbjct: 431 GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMP 490

Query: 619 DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG 678
           DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG
Sbjct: 491 DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG 550

Query: 679 ISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG 738
           ISKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG
Sbjct: 551 ISKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG 610

Query: 739 RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS 798
           RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS
Sbjct: 611 RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS 670

Query: 799 ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK 858
           ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK
Sbjct: 671 ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK 730

Query: 859 YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR 918
           YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR
Sbjct: 731 YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR 790

Query: 919 QEECFQKYFKSHIE 933
           QEECFQKYFKSHIE
Sbjct: 791 QEECFQKYFKSHIE 799

BLAST of MS010674 vs. NCBI nr
Match: XP_022133367.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 [Momordica charantia])

HSP 1 Score: 1558.9 bits (4035), Expect = 0.0e+00
Identity = 787/793 (99.24%), Postives = 787/793 (99.24%), Query Frame = 0

Query: 140 KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS 199
           KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS
Sbjct: 4   KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS 63

Query: 200 SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC 259
           SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC
Sbjct: 64  SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC 123

Query: 260 PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG 319
           PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG
Sbjct: 124 PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG 183

Query: 320 NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN 379
           NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN
Sbjct: 184 NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN 243

Query: 380 ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL 439
           ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL
Sbjct: 244 ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL 303

Query: 440 SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL 499
           SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL
Sbjct: 304 SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL 363

Query: 500 FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG 559
           FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG
Sbjct: 364 FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG 423

Query: 560 VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPD 619
           VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMPD
Sbjct: 424 VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMPD 483

Query: 620 LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI 679
           LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI
Sbjct: 484 LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI 543

Query: 680 SKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR 739
           SKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR
Sbjct: 544 SKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR 603

Query: 740 HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE 799
           HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE
Sbjct: 604 HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE 663

Query: 800 TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY 859
           TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY
Sbjct: 664 TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY 723

Query: 860 DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ 919
           DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ
Sbjct: 724 DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ 783

Query: 920 EECFQKYFKSHIE 933
           EECFQKYFKSHIE
Sbjct: 784 EECFQKYFKSHIE 791

BLAST of MS010674 vs. NCBI nr
Match: XP_022133368.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X4 [Momordica charantia])

HSP 1 Score: 1514.6 bits (3920), Expect = 0.0e+00
Identity = 765/771 (99.22%), Postives = 765/771 (99.22%), Query Frame = 0

Query: 162 MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV 221
           MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV
Sbjct: 1   MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV 60

Query: 222 EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL 281
           EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL
Sbjct: 61  EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL 120

Query: 282 DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ 341
           DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ
Sbjct: 121 DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ 180

Query: 342 NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV 401
           NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV
Sbjct: 181 NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV 240

Query: 402 FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS 461
           FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS
Sbjct: 241 FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS 300

Query: 462 VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE 521
           VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE
Sbjct: 301 VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE 360

Query: 522 GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL 581
           GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL
Sbjct: 361 GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL 420

Query: 582 IQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV 641
           IQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV
Sbjct: 421 IQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV 480

Query: 642 AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAG 701
           AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT     G
Sbjct: 481 AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT-----G 540

Query: 702 CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL 761
           CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL
Sbjct: 541 CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL 600

Query: 762 VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES 821
           VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES
Sbjct: 601 VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES 660

Query: 822 GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN 881
           GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN
Sbjct: 661 GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN 720

Query: 882 EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
           EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE
Sbjct: 721 EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 766

BLAST of MS010674 vs. NCBI nr
Match: XP_038883514.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1397.1 bits (3615), Expect = 0.0e+00
Identity = 711/932 (76.29%), Postives = 794/932 (85.19%), Query Frame = 0

Query: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60
           M C F  RLW+LLLIILRF S +G S+S +NS+FSVL+PY DNSEG+AR GGRSLLSLPL
Sbjct: 1   MNCHFFRRLWLLLLIILRFWSFLGSSKSSDNSEFSVLNPYWDNSEGVARIGGRSLLSLPL 60

Query: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120
           K K +TAL+AA DGAIHLVDSNSM  IWSF+SGPPIYSSYQA I H+HNQENASG+S SF
Sbjct: 61  KSKSSTALIAALDGAIHLVDSNSMKIIWSFSSGPPIYSSYQAIINHEHNQENASGVSRSF 120

Query: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180
           FFDCGDDWELY+HTEHGKMKLPS+IDEVVRNTPYIFEDGAVM GSRKTTVFEVDPVTG L
Sbjct: 121 FFDCGDDWELYIHTEHGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGDL 180

Query: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240
           IRNH+S+  SSEL+NE    Y SK+NLD KDL+Q  V++PV PRLYITRTDYSLKSSFS 
Sbjct: 181 IRNHMSNSLSSELNNEGIGGYKSKKNLDIKDLMQSAVMNPVGPRLYITRTDYSLKSSFSN 240

Query: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300
           SEEV+WSLNVAEIGATLLCP+VENP + +PW LQ+N SF +DFAMPLSCQSK +VFRERS
Sbjct: 241 SEEVSWSLNVAEIGATLLCPDVENPIEGIPWILQNNNSFGVDFAMPLSCQSKALVFRERS 300

Query: 301 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP 360
           H  SG SGYKRLSEAHNI NMSLVPASG  LPSQL V E  +THPEK ML GP       
Sbjct: 301 HFLSGPSGYKRLSEAHNIDNMSLVPASGSLLPSQLNVAEHIDTHPEKFMLHGPV------ 360

Query: 361 LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK 420
              N +S AV  MP    NESS++Q  KMG   G  GLFFVFL+T+LVGL+ + R L  +
Sbjct: 361 ---NIASSAVGSMP---FNESSIIQEQKMGARPGAVGLFFVFLITMLVGLMMYGRTLAVQ 420

Query: 421 VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW 480
           VKQFFL+EK SSTSNS+++SSKKNK RKSKR+ NS KRD+S+SSE  DM M SE N+NN 
Sbjct: 421 VKQFFLKEKSSSTSNSRVISSKKNKPRKSKRSANSGKRDVSISSEIGDMFMQSEDNLNNG 480

Query: 481 FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 540
           FH++NL + A +GR IGKLF+TNKEIA GSNGTI+LEG+YEGRPVAVKRLVKTHHDVA K
Sbjct: 481 FHNNNLTNIASSGRWIGKLFITNKEIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASK 540

Query: 541 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDT 600
           EVQNLI SDRHPNIVRWYG++SDQDFVYLSLERCTCSLDDLIQI S+ S N M  LD+ T
Sbjct: 541 EVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSNLSMNYMPDLDRAT 600

Query: 601 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 660
             M +Y +HLES+KD MP+LKLWKE G P+SVLLKLMRD+VAG+EHLHELGIIHRDLKPQ
Sbjct: 601 GRMDEYNMHLESIKDSMPNLKLWKETGRPTSVLLKLMRDVVAGLEHLHELGIIHRDLKPQ 660

Query: 661 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR 720
           NVLI+KQ+S+C+KLSDMGISKRL A++SSLGH AT     GCGSSGWQAPEQLL GRQTR
Sbjct: 661 NVLIVKQQSICSKLSDMGISKRLPANVSSLGHRAT-----GCGSSGWQAPEQLLRGRQTR 720

Query: 721 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 780
           A+D FSLGCVLFFCITGGRHPFGD LERDVNIVKN+M+L LV +IPEAVDLISQLLNP+P
Sbjct: 721 AIDSFSLGCVLFFCITGGRHPFGDHLERDVNIVKNKMNLLLVDNIPEAVDLISQLLNPNP 780

Query: 781 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 840
            LRPKASEVL+HPLFWSSE RLSFLRDTSDR+ELEDR  E+ LLKALE TA  ALGT+WD
Sbjct: 781 SLRPKASEVLRHPLFWSSEMRLSFLRDTSDRIELEDR--ETNLLKALESTAPQALGTEWD 840

Query: 841 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 900
           EKMEPIFI NIGRYRRYKY SVRDLLRVMRNKLNHYRELP EIQELIGS+PEGFD YF  
Sbjct: 841 EKMEPIFIANIGRYRRYKYYSVRDLLRVMRNKLNHYRELPEEIQELIGSIPEGFDDYFTR 900

Query: 901 RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
           RFPRLLIEVYK+IS +CRQEECFQKYFKSH++
Sbjct: 901 RFPRLLIEVYKVISWFCRQEECFQKYFKSHVD 913

BLAST of MS010674 vs. ExPASy Swiss-Prot
Match: Q9C5S2 (Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana OX=3702 GN=IRE1A PE=1 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 7.1e-202
Identity = 424/909 (46.64%), Postives = 559/909 (61.50%), Query Frame = 0

Query: 36  VLSPYGDNSEGL------ARTGGRSLLSLPLK--GKINTALVAASDGAIHLVDSNSMNKI 95
           ++SP G  ++ +      +  G RS+L +  +   + NT LV   DG + L         
Sbjct: 22  IMSPCGGAADDVTYPIVPSSPGRRSILQIRREPPTEPNTKLVVDRDGKVFLKQQPKETPY 81

Query: 96  WSFTSGPPIYSSYQAHIEHDHNQENASGLSSSFFFDCGDDWELYMHTEHGKMKLPS---- 155
           WSF++G P++S YQA    ++N ENA+ ++             Y++       +      
Sbjct: 82  WSFSTGSPMHSLYQA--PANNNTENATEITRPHII------VEYLNNSKAATTVDGYHNW 141

Query: 156 SIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNS 215
           ++ E  R  P + +DG V +GS  T+ + VD  +G+LI  + S    ++++N        
Sbjct: 142 TVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSGRLIHVYKST-GDTKITNA------- 201

Query: 216 KQNLDNKDLIQPGVVSP-VEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEV 275
                   L++P      V   L I RTD  L+     + ++ W+L V+   A LLC  V
Sbjct: 202 --------LVKPASTEDFVNEPLLIRRTDSKLEHFSKTTGKLVWNLTVSHFRAALLCDPV 261

Query: 276 ENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMS 335
            N    +   LQ+         MPL C S++ V                           
Sbjct: 262 FNSGYDLGPKLQTG------IYMPLLCGSQIDV--------------------------- 321

Query: 336 LVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESS 395
                GP +  ++  D+  N      MLP P+ N       +  S+  I MP  K  ES 
Sbjct: 322 ----RGPEIVIRVLHDQPMNVK----MLPSPSLN-------HFESENSI-MPFGKARESR 381

Query: 396 MVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSK 455
            +Q          FG +    V +L  LV     ++  +K+F  R    S  + K   SK
Sbjct: 382 KLQEQHKQKYTYLFGQWSP--VKLLAPLVLLGVVVSVFIKKFSSR---GSDVSLKAGPSK 441

Query: 456 KNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVT 515
           K K RKS ++ N      SV    +   +   G +   F  +N    A  GR+IGKLF++
Sbjct: 442 KKKNRKSAKDTNRQ----SVPRGQDQFELIEGGQMLLGF--NNFQSGATDGRKIGKLFLS 501

Query: 516 NKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVES 575
           +KEIA+GSNGT+V EG+YEGRPVAVKRLV++HH+VAFKE+QNLIASD+H NI+RWYGVE 
Sbjct: 502 SKEIAKGSNGTVVFEGIYEGRPVAVKRLVRSHHEVAFKEIQNLIASDQHTNIIRWYGVEY 561

Query: 576 DQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKL 635
           DQDFVYLSLERCTCSLDDLI+   + S   +L  +  TE +  Y++ L+S++ V+     
Sbjct: 562 DQDFVYLSLERCTCSLDDLIKSYLEFSMTKVLENNDSTEGVAAYKIQLDSLEGVIKGNNF 621

Query: 636 WKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKR 695
           WK  GHPS ++LKLMRD+V G+ HLHELGI+HRDLKPQNVLI K  ++ AKLSDMGISKR
Sbjct: 622 WKVGGHPSPLMLKLMRDIVCGIVHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKR 681

Query: 696 LLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPF 755
           +  DMSSLGH ATG+     GSSGWQAPEQLL GRQTRAVD+FSLGCV+F+ ITG +HPF
Sbjct: 682 MSRDMSSLGHLATGS-----GSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPF 741

Query: 756 GDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRL 815
           GD LERDVNIVKN++DLFLV+ +PEA DLIS+LLNPDPDLRP A+EVL HP+FW+SE RL
Sbjct: 742 GDDLERDVNIVKNKVDLFLVEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRL 801

Query: 816 SFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSV 875
           SFLRD SDRVELE+RE++S +LKA+E TA +A+G KWDEK+EP+FITNIGRYRRYKYDS+
Sbjct: 802 SFLRDASDRVELENREADSEILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSI 840

Query: 876 RDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEEC 932
           RDLLRV+RNKLNH+RELP EIQEL+G+VPEGFD YFA RFP+LLIEVY++IS +CR+EE 
Sbjct: 862 RDLLRVIRNKLNHHRELPPEIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEV 840

BLAST of MS010674 vs. ExPASy Swiss-Prot
Match: Q93VJ2 (Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana OX=3702 GN=IRE1B PE=2 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 6.9e-189
Identity = 403/884 (45.59%), Postives = 550/884 (62.22%), Query Frame = 0

Query: 68  LVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQA-HIEHDHNQENASGLSSSFFFDCGD 127
           LV+  DG+I LVD +S    W+F +  PIYSSYQA H  +  ++E +S L   F+ DC  
Sbjct: 50  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 109

Query: 128 DWELYMHTEHGKMKLPSSID--EVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNH 187
           DW LY  +     ++   +D  E +   PY   D  +++G + T+VF +D  TGKL++ +
Sbjct: 110 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 169

Query: 188 ISD--FSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVE-PRL-YITRTDYSLKSSFSG 247
             D  +S++ + N++  +    +  +   L   G     + P L YI R D+ ++   S 
Sbjct: 170 RMDELYSNTVVENDKEKAIVLSK--EAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CISK 229

Query: 248 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 307
             +V WS++ A++ A L   E       +  ++  N         PLS  + V + + R 
Sbjct: 230 FGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKN-------QFPLSYTTSVPMVQLR- 289

Query: 308 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPG---PAPNI 367
                +  Y+ L       + +L        P+QL + +       +L LPG       +
Sbjct: 290 -----NVKYETLFPRLGFLDEALYLPFQDRKPNQLAIGD-----GNQLTLPGNKEAEEVL 349

Query: 368 SSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTIL--VGLVTFSR 427
           S PLP    S+      +  I + S  Q       +G   L F F VT+L   GL  +  
Sbjct: 350 SLPLPETVISQ------ITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFFYRL 409

Query: 428 GLTGKVKQFFLREKLSSTSNSKILSSKKN---KARKSKRN--VNSDKRDISVSSENEDML 487
             + ++K+ ++ E   +T   K   SKKN   KA   K N  ++   +D S   ENE  L
Sbjct: 410 RQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVHKKENGFISGGNKDPS-HEENEKRL 469

Query: 488 MPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRL 547
           + +   +NN         ++  G R+GKLFV+NKEIA+GSNGT+VLEG YEGR VAVKRL
Sbjct: 470 LTAFPGLNN---------SSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 529

Query: 548 VKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSF 607
           V++HHDVA KE+ NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S    
Sbjct: 530 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS---- 589

Query: 608 NSMLGLDQDTEPMIKYRLHLESVKDVMPD---LKLWKENGHPSSVLLKLMRDMVAGVEHL 667
            ++L       PM    +H   +  +  +   ++LWKENGHPS VLLKLMRD+VAG+ HL
Sbjct: 590 -ALL-----ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHL 649

Query: 668 HELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGW 727
           H++GI+HRDLKPQNVLI+K  S+CAKLSDMGISKRL AD S+L  ++TG    G GSSGW
Sbjct: 650 HDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG---LGSGSSGW 709

Query: 728 QAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPE 787
           QAPEQL + RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERDVN++ +Q DLFL++S+PE
Sbjct: 710 QAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPE 769

Query: 788 AVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKAL 847
           AV L++ LLNPDP+LRP+A +V+ HPLFW+S+ RLSFLRD SDRVELE+RE  S LL AL
Sbjct: 770 AVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAAL 829

Query: 848 EKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELI 907
           E TA + L  +WDEK++ IF+ NIGRYRRYK+DS+RDLLRV+RNKLNHYRELP E+QEL+
Sbjct: 830 ESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELL 880

Query: 908 GSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHI 932
           GSVPEGF+ YF+SRFP+LLI+VY ++  YC  EE F KY K+ +
Sbjct: 890 GSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 880

BLAST of MS010674 vs. ExPASy Swiss-Prot
Match: Q7XIT1 (Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. japonica OX=39947 GN=IRE1 PE=1 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.0e-155
Identity = 350/867 (40.37%), Postives = 481/867 (55.48%), Query Frame = 0

Query: 73  DGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSFFFDCGDDWELYM 132
           +G I LVD  S    W F++GPP+           H   +   L+   + D  +  +L  
Sbjct: 105 NGTIQLVDLLSKLPRWQFSTGPPL---------SKHITTSKPDLNYVIYLDGSETSDLIE 164

Query: 133 HTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSE 192
                 ++LP  ++E +  TPYI  D  V IGS+ +T F V+  +G++I  H    + +E
Sbjct: 165 VHNGSGVRLPWKLEEFIAETPYI-RDSFVTIGSKVSTTFVVNADSGEIIYKHSLPVALNE 224

Query: 193 LSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLN--- 252
           +         SK      D  + G  + +   + + RTDYS+ +S  G     W+     
Sbjct: 225 VGGPLVEEIPSK-----LDAARSGTSANI---IVVVRTDYSISASDLGEHLFNWTRTSFT 284

Query: 253 ---VAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGS 312
               A  G   +  +      ++P  +++ G   +   +P S     +V R         
Sbjct: 285 ANYYARYGHQDMLAQSSCLRGNIPC-IRTEGP-PIKLYLPDSSSDNAIVLRP-------- 344

Query: 313 SGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSS 372
                      +  +S V A  P LP            P+K  LP PA   +  L    +
Sbjct: 345 -----------VNEVSAVDALEPLLP------------PKK--LPQPAGESNVALDSAQN 404

Query: 373 SKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLV-TILVGLVTFSRGLTGKV---- 432
             A I +      ++ +       +    F  F + L+   LV L   S+     V    
Sbjct: 405 QTADIALGHFVPADTELTNSVTKFSYRWLFPTFLMLLIMACLVKLADASKYCRQFVIRFL 464

Query: 433 KQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWF 492
           K F   EKL         +SK+ KARK    +NS +   +   E       +E   N   
Sbjct: 465 KPFMRDEKLMDPRGKSEGTSKRRKARKKDGLINSTQIFSASDKEGNGTGGSTEAQSNKAH 524

Query: 493 HHSNLP-DNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 552
             +N+   N   GR+IGKL V +KEI +GSNGT+V EG Y GR VAVKRL+++H+D+A K
Sbjct: 525 DSTNVELPNGLNGRQIGKLCVYSKEIGKGSNGTVVFEGSYGGREVAVKRLLRSHNDIASK 584

Query: 553 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDT 612
           E++NLIASD+ PNIVR YG E D DFVY+SLERC CSL DLIQ+ S   F++  G     
Sbjct: 585 EIENLIASDQDPNIVRMYGFEQDNDFVYISLERCRCSLADLIQLHSVPPFSNTKG----- 644

Query: 613 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 672
                             D++LW+++G PS+ LLKLMRD+VAG+ HLH LGIIHRDLKPQ
Sbjct: 645 -----------------TDIELWRQDGLPSAQLLKLMRDVVAGIVHLHSLGIIHRDLKPQ 704

Query: 673 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR 732
           NVLI K+  + AKLSDMGISKRL  DM+S+ HH T     G GSSGWQAPEQL HGRQTR
Sbjct: 705 NVLISKEGPLRAKLSDMGISKRLQEDMTSVSHHGT-----GFGSSGWQAPEQLRHGRQTR 764

Query: 733 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 792
           A+DLFSLGC++F+CIT G+HPFG+  ERD+ I+ NQ DLF+V  IPEAV LISQLL+PDP
Sbjct: 765 AIDLFSLGCLIFYCITKGKHPFGEYYERDMKIINNQFDLFIVDHIPEAVHLISQLLDPDP 824

Query: 793 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 852
           + RP A  V+ HP FWS E  LSFLRDTSDR+E   + SE+ L+ ALE     A G  W 
Sbjct: 825 EKRPTAVYVMHHPFFWSPELCLSFLRDTSDRIE---KTSETDLIDALEGINVEAFGKNWG 884

Query: 853 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 912
           EK++   + ++GRYR+Y ++S RDLLR++RNK  HYRE  ++++EL+GS+PEGF  YF+S
Sbjct: 885 EKLDAALLADMGRYRKYSFESTRDLLRLIRNKSGHYREFSDDLKELLGSLPEGFVQYFSS 888

Query: 913 RFPRLLIEVYKIISQYCRQEECFQKYF 928
           RFP+LLI+VY+++S++C+ EE F KYF
Sbjct: 945 RFPKLLIKVYEVMSEHCKDEEAFSKYF 888

BLAST of MS010674 vs. ExPASy Swiss-Prot
Match: Q9SF12 (Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidopsis thaliana OX=3702 GN=At3g11870 PE=3 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 1.7e-86
Identity = 221/566 (39.05%), Postives = 319/566 (56.36%), Query Frame = 0

Query: 403 LVTILVGLVTFS--RGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSD---- 462
           LV ++   + FS  +GL G V +    +K    S  ++ +S ++  +  +    SD    
Sbjct: 12  LVVVVCVFLRFSKDKGLDGIVNE----KKRDKNSAPRVSASGEDGTKNEQVEKKSDPSGG 71

Query: 463 --KRDISVSSENEDMLMPSEGNVNNW-------FHHSNLPDNAGTGRRIG-KLFVTNKEI 522
             + +   +SE++ + +PS+ N+N                +N   G+ +  +L V+  E+
Sbjct: 72  LGEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRRLLVSTNEM 131

Query: 523 ARGSNGTIVLEGVYEGR-PVAVKRLVKTHHDVAF--KEVQNLIASDRHPNIVRWYGVESD 582
             G NG  V +GVY  R  VAVK L   H   AF   E+ N    D H NI+R++G+E D
Sbjct: 132 KYGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNIIRFHGLEQD 191

Query: 583 QDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKLW 642
           Q F Y+ LE   CSLDDLI++       S+    +DT+ +      ++ ++ VM  +K W
Sbjct: 192 QSFAYICLEPWKCSLDDLIKL-------SVRRTKRDTQAVAP----VDDLEKVMKRIKFW 251

Query: 643 KENGHPSSV--LLKLMRDMVAGVEHLHELGIIHRDLKPQNVLII-KQKSMCAKLSDMGIS 702
           KE G P  +  +LKLMRD+V G+ HLH+L  IHR+L PQNVLII K  ++ AK+SDM +S
Sbjct: 252 KEKGKPLPLTPMLKLMRDVVCGLAHLHKLKTIHRNLNPQNVLIIVKDMTLTAKISDMSLS 311

Query: 703 KRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR---AVDLFSLGCVLFFCITG 762
           K L    SS  H AT +     GSSGWQAPEQL   ++ +     D+F+ GC+L + +  
Sbjct: 312 KHLGGKKSSYKHLATCS-----GSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM- 371

Query: 763 GRHPFGDRLERDVNI-VKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFW 822
           G HPFG   ERD NI   N+ +L LV ++ EA++LI QLLN  PDLRP A++VL HPLFW
Sbjct: 372 GTHPFGSPSERDTNIKTNNKTNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFW 431

Query: 823 SSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRY-- 882
            SE RL FLR+ SDR+EL+   +  G L        L     W  K+   FIT+I     
Sbjct: 432 DSEKRLFFLREASDRIELD--ITMWGDLNKTIAPRVLGESKDWASKLGKTFITHIENLAQ 491

Query: 883 -----------RRYKYDSVRDLLRVMRNKLNHYRELPNE--IQELIGSVPEGFDGYFASR 928
                      R YKY S+R LLR++RN L+H+RE+ ++  I+E++G VPEG D +F +R
Sbjct: 492 AQPGQESRQYNRSYKYWSLRHLLRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTAR 551

BLAST of MS010674 vs. ExPASy Swiss-Prot
Match: P32361 (Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=IRE1 PE=1 SV=2)

HSP 1 Score: 289.3 bits (739), Expect = 1.6e-76
Identity = 174/523 (33.27%), Postives = 270/523 (51.63%), Query Frame = 0

Query: 456  DKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIV 515
            D++ + +++E +     S G        S + +     + +  L V+ K +  GS+GT+V
Sbjct: 632  DEKSLDLTTEKKKRKRGSRGGKKG--RKSRIANIPNFEQSLKNLVVSEKILGYGSSGTVV 691

Query: 516  LEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCT 575
             +G ++GRPVAVKR++    D+A  E++ L  SD HPN++R+Y  E+   F+Y++LE C 
Sbjct: 692  FQGSFQGRPVAVKRMLIDFCDIALMEIKLLTESDDHPNVIRYYCSETTDRFLYIALELCN 751

Query: 576  CSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLK 635
             +L DL++                           ++V D   +LKL KE        + 
Sbjct: 752  LNLQDLVE--------------------------SKNVSD--ENLKLQKEYNP-----IS 811

Query: 636  LMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAK-----------LSDMGISKRLL 695
            L+R + +GV HLH L IIHRDLKPQN+L+       A            +SD G+ K+L 
Sbjct: 812  LLRQIASGVAHLHSLKIIHRDLKPQNILVSTSSRFTADQQTGAENLRILISDFGLCKKLD 871

Query: 696  ADMSSLGHHATGNTTAGCGSSGWQAPEQLLHG---------------------------- 755
            +  SS       N     G+SGW+APE L                               
Sbjct: 872  SGQSSF----RTNLNNPSGTSGWRAPELLEESNNLQCQVETEHSSSRHTVVSSDSFYDPF 931

Query: 756  ---RQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQS------IPE 815
               R TR++D+FS+GCV ++ ++ G+HPFGD+  R+ NI++    L  ++       I E
Sbjct: 932  TKRRLTRSIDIFSMGCVFYYILSKGKHPFGDKYSRESNIIRGIFSLDEMKCLHDRSLIAE 991

Query: 816  AVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKAL 875
            A DLISQ+++ DP  RP A +VL+HPLFW    +L FL   SDR+E+E+R+  S LL   
Sbjct: 992  ATDLISQMIDHDPLKRPTAMKVLRHPLFWPKSKKLEFLLKVSDRLEIENRDPPSALLMKF 1051

Query: 876  EKTAQLALGT-KWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQEL 930
            +  +   + +  W  K +  F+ N+ RYR+Y    + DLLR +RNK +H+ +LP +I EL
Sbjct: 1052 DAGSDFVIPSGDWTVKFDKTFMDNLERYRKYHSSKLMDLLRALRNKYHHFMDLPEDIAEL 1111

BLAST of MS010674 vs. ExPASy TrEMBL
Match: A0A6J1BYY0 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111005957 PE=4 SV=1)

HSP 1 Score: 1838.2 bits (4760), Expect = 0.0e+00
Identity = 926/932 (99.36%), Postives = 926/932 (99.36%), Query Frame = 0

Query: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60
           MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL
Sbjct: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60

Query: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120
           KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF
Sbjct: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120

Query: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180
           FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL
Sbjct: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180

Query: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240
           IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG
Sbjct: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240

Query: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300
           SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS
Sbjct: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300

Query: 301 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP 360
           HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP
Sbjct: 301 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSP 360

Query: 361 LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK 420
           LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK
Sbjct: 361 LPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGK 420

Query: 421 VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW 480
           VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW
Sbjct: 421 VKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNW 480

Query: 481 FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 540
           FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK
Sbjct: 481 FHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFK 540

Query: 541 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDT 600
           EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDT
Sbjct: 541 EVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDT 600

Query: 601 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 660
           EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ
Sbjct: 601 EPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQ 660

Query: 661 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR 720
           NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTR
Sbjct: 661 NVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTR 720

Query: 721 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 780
           AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP
Sbjct: 721 AVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDP 780

Query: 781 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 840
           DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD
Sbjct: 781 DLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWD 840

Query: 841 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 900
           EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS
Sbjct: 841 EKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFAS 900

Query: 901 RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
           RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE
Sbjct: 901 RFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 927

BLAST of MS010674 vs. ExPASy TrEMBL
Match: A0A6J1BUW5 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005957 PE=4 SV=1)

HSP 1 Score: 1560.8 bits (4040), Expect = 0.0e+00
Identity = 788/794 (99.24%), Postives = 788/794 (99.24%), Query Frame = 0

Query: 139 MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH 198
           MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH
Sbjct: 11  MKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEH 70

Query: 199 SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL 258
           SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL
Sbjct: 71  SSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLL 130

Query: 259 CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI 318
           CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI
Sbjct: 131 CPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNI 190

Query: 319 GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI 378
           GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI
Sbjct: 191 GNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKI 250

Query: 379 NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI 438
           NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI
Sbjct: 251 NESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKI 310

Query: 439 LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK 498
           LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK
Sbjct: 311 LSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGK 370

Query: 499 LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY 558
           LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY
Sbjct: 371 LFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWY 430

Query: 559 GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMP 618
           GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMP
Sbjct: 431 GVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMP 490

Query: 619 DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG 678
           DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG
Sbjct: 491 DLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMG 550

Query: 679 ISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG 738
           ISKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG
Sbjct: 551 ISKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGG 610

Query: 739 RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS 798
           RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS
Sbjct: 611 RHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSS 670

Query: 799 ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK 858
           ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK
Sbjct: 671 ETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYK 730

Query: 859 YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR 918
           YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR
Sbjct: 731 YDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCR 790

Query: 919 QEECFQKYFKSHIE 933
           QEECFQKYFKSHIE
Sbjct: 791 QEECFQKYFKSHIE 799

BLAST of MS010674 vs. ExPASy TrEMBL
Match: A0A6J1BWG9 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111005957 PE=4 SV=1)

HSP 1 Score: 1558.9 bits (4035), Expect = 0.0e+00
Identity = 787/793 (99.24%), Postives = 787/793 (99.24%), Query Frame = 0

Query: 140 KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS 199
           KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS
Sbjct: 4   KLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHS 63

Query: 200 SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC 259
           SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC
Sbjct: 64  SYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLC 123

Query: 260 PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG 319
           PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG
Sbjct: 124 PEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIG 183

Query: 320 NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN 379
           NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN
Sbjct: 184 NMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKIN 243

Query: 380 ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL 439
           ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL
Sbjct: 244 ESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKIL 303

Query: 440 SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL 499
           SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL
Sbjct: 304 SSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKL 363

Query: 500 FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG 559
           FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG
Sbjct: 364 FVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYG 423

Query: 560 VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPD 619
           VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMPD
Sbjct: 424 VESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMPD 483

Query: 620 LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI 679
           LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI
Sbjct: 484 LKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGI 543

Query: 680 SKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR 739
           SKRLLADMSSLGHHAT     GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR
Sbjct: 544 SKRLLADMSSLGHHAT-----GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGR 603

Query: 740 HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE 799
           HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE
Sbjct: 604 HPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSE 663

Query: 800 TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY 859
           TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY
Sbjct: 664 TRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKY 723

Query: 860 DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ 919
           DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ
Sbjct: 724 DSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQ 783

Query: 920 EECFQKYFKSHIE 933
           EECFQKYFKSHIE
Sbjct: 784 EECFQKYFKSHIE 791

BLAST of MS010674 vs. ExPASy TrEMBL
Match: A0A6J1BYY4 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X4 OS=Momordica charantia OX=3673 GN=LOC111005957 PE=4 SV=1)

HSP 1 Score: 1514.6 bits (3920), Expect = 0.0e+00
Identity = 765/771 (99.22%), Postives = 765/771 (99.22%), Query Frame = 0

Query: 162 MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV 221
           MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV
Sbjct: 1   MIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPV 60

Query: 222 EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL 281
           EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL
Sbjct: 61  EPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNL 120

Query: 282 DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ 341
           DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ
Sbjct: 121 DFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQ 180

Query: 342 NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV 401
           NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV
Sbjct: 181 NTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFV 240

Query: 402 FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS 461
           FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS
Sbjct: 241 FLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSDKRDIS 300

Query: 462 VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE 521
           VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE
Sbjct: 301 VSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYE 360

Query: 522 GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL 581
           GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL
Sbjct: 361 GRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDL 420

Query: 582 IQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV 641
           IQICSDQSFNSM GLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV
Sbjct: 421 IQICSDQSFNSMPGLDQDTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMV 480

Query: 642 AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAG 701
           AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT     G
Sbjct: 481 AGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT-----G 540

Query: 702 CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL 761
           CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL
Sbjct: 541 CGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFL 600

Query: 762 VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES 821
           VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES
Sbjct: 601 VQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESES 660

Query: 822 GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN 881
           GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN
Sbjct: 661 GLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPN 720

Query: 882 EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
           EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE
Sbjct: 721 EIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 766

BLAST of MS010674 vs. ExPASy TrEMBL
Match: A0A1S3B291 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103485194 PE=4 SV=1)

HSP 1 Score: 1390.6 bits (3598), Expect = 0.0e+00
Identity = 702/934 (75.16%), Postives = 800/934 (85.65%), Query Frame = 0

Query: 1   MKCRFSCRLWVLLLIILRFRSSIGESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPL 60
           MKCRF C   +LLL+ILRF S +  S+S ENS+FSVL+PYG NSEG+ R GGRSLLSLPL
Sbjct: 1   MKCRFFC---LLLLLILRFWSFLVSSKSFENSEFSVLNPYGVNSEGVTRIGGRSLLSLPL 60

Query: 61  KGKINTALVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSF 120
           KGK +TAL+AA DG IHLVDSNSM  IWSF+SGPPIYSSYQA+I H+HNQENASG+ SSF
Sbjct: 61  KGKSSTALIAALDGTIHLVDSNSMKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSF 120

Query: 121 FFDCGDDWELYMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKL 180
           FFDCGDDWELY+HTEHGKMKLPS+IDEVVRNTPYIFEDGAVM GSRKTTVFEVD VTG+L
Sbjct: 121 FFDCGDDWELYIHTEHGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGEL 180

Query: 181 IRNHISDFSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSG 240
           IRNH+S F SSELSNEE  SY SKQN+D KDL+Q  +++PVEPRLYITRTDYSLKSSFS 
Sbjct: 181 IRNHMSKFLSSELSNEEPGSYKSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSN 240

Query: 241 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 300
           SEE +WSLNVAEIGATLLCP+VENP + +PW LQ+N SF +DFAMPLSCQSK +VFR+RS
Sbjct: 241 SEEASWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRS 300

Query: 301 HVFSGSSGYKRL-SEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISS 360
           H  SG SGYKRL SEAHN  N+SLV ASG  LPSQL V +  NT  EK ML GP      
Sbjct: 301 HFLSGPSGYKRLPSEAHNSNNISLVSASGSFLPSQLTVGKHINTGSEKFMLTGPV----- 360

Query: 361 PLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTG 420
               N++S  V+P+P MKINES+++Q  KMGTL G FGLFFVFL+T+LVGL+ + R L  
Sbjct: 361 ----NNTSYRVVPLPSMKINESNIIQEQKMGTLPGAFGLFFVFLLTMLVGLMKYGRTLAV 420

Query: 421 KVKQFFLREKLS-STSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVN 480
           KVKQ FL+E+ S  TSNS+++SSKKNK RKSK++ +S KR++S+SSE EDML+  E N+N
Sbjct: 421 KVKQSFLKEQSSLGTSNSRVISSKKNKPRKSKKSGSSGKREVSISSEIEDMLLQREDNLN 480

Query: 481 NWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVA 540
           N FH +NL + AG+GRR+GKL+VTNKEIA GSNGTI+LEG+YEGRPVAVKRLVKTHHD+ 
Sbjct: 481 NGFHDNNLTNIAGSGRRVGKLWVTNKEIATGSNGTIILEGIYEGRPVAVKRLVKTHHDIG 540

Query: 541 FKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQ 600
            KEVQNLI SDRHPNIVRWYG++SDQDFVYLSLERCTCSL DLI+I SD   NSMLGLDQ
Sbjct: 541 SKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLYDLIKIYSDLPMNSMLGLDQ 600

Query: 601 DTEPMIKYRLHLESVKDVMPDLKLWKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLK 660
           DT  M +Y +HLES+K  MP+LKLW ENG PSSVLLKLMRD+VAG+EHLHELGIIHRDLK
Sbjct: 601 DTGRMDEYNIHLESIKVAMPNLKLWNENGRPSSVLLKLMRDIVAGLEHLHELGIIHRDLK 660

Query: 661 PQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQ 720
           PQNVLI+KQ+S+C+KLSDMGISKRL A++SSLGH AT     GCGSSGWQAPEQLLHGRQ
Sbjct: 661 PQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRAT-----GCGSSGWQAPEQLLHGRQ 720

Query: 721 TRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNP 780
           TRA+DLFSLGCVLFFC+TGGRHPFGD LERDVNIV N+M+L LV +IPE VDLISQLLNP
Sbjct: 721 TRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLISQLLNP 780

Query: 781 DPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTK 840
           +P LRPKASEVLQHPLFWS E RLSFLRDTSDR+ELEDRE++  LLKALE TAQ+ALG K
Sbjct: 781 NPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETD--LLKALESTAQIALGLK 840

Query: 841 WDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYF 900
           W+EK+EPIFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELP EIQEL+GS+P GFD YF
Sbjct: 841 WNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPVGFDDYF 900

Query: 901 ASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 933
             RFP+LLIEVYK+IS +CRQEECFQKYFKSH++
Sbjct: 901 TRRFPKLLIEVYKVISCFCRQEECFQKYFKSHVD 915

BLAST of MS010674 vs. TAIR 10
Match: AT2G17520.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 705.7 bits (1820), Expect = 5.0e-203
Identity = 424/909 (46.64%), Postives = 559/909 (61.50%), Query Frame = 0

Query: 36  VLSPYGDNSEGL------ARTGGRSLLSLPLK--GKINTALVAASDGAIHLVDSNSMNKI 95
           ++SP G  ++ +      +  G RS+L +  +   + NT LV   DG + L         
Sbjct: 22  IMSPCGGAADDVTYPIVPSSPGRRSILQIRREPPTEPNTKLVVDRDGKVFLKQQPKETPY 81

Query: 96  WSFTSGPPIYSSYQAHIEHDHNQENASGLSSSFFFDCGDDWELYMHTEHGKMKLPS---- 155
           WSF++G P++S YQA    ++N ENA+ ++             Y++       +      
Sbjct: 82  WSFSTGSPMHSLYQA--PANNNTENATEITRPHII------VEYLNNSKAATTVDGYHNW 141

Query: 156 SIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSSSELSNEEHSSYNS 215
           ++ E  R  P + +DG V +GS  T+ + VD  +G+LI  + S    ++++N        
Sbjct: 142 TVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSGRLIHVYKST-GDTKITNA------- 201

Query: 216 KQNLDNKDLIQPGVVSP-VEPRLYITRTDYSLKSSFSGSEEVAWSLNVAEIGATLLCPEV 275
                   L++P      V   L I RTD  L+     + ++ W+L V+   A LLC  V
Sbjct: 202 --------LVKPASTEDFVNEPLLIRRTDSKLEHFSKTTGKLVWNLTVSHFRAALLCDPV 261

Query: 276 ENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYKRLSEAHNIGNMS 335
            N    +   LQ+         MPL C S++ V                           
Sbjct: 262 FNSGYDLGPKLQTG------IYMPLLCGSQIDV--------------------------- 321

Query: 336 LVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAVIPMPLMKINESS 395
                GP +  ++  D+  N      MLP P+ N       +  S+  I MP  K  ES 
Sbjct: 322 ----RGPEIVIRVLHDQPMNVK----MLPSPSLN-------HFESENSI-MPFGKARESR 381

Query: 396 MVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKLSSTSNSKILSSK 455
            +Q          FG +    V +L  LV     ++  +K+F  R    S  + K   SK
Sbjct: 382 KLQEQHKQKYTYLFGQWSP--VKLLAPLVLLGVVVSVFIKKFSSR---GSDVSLKAGPSK 441

Query: 456 KNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVT 515
           K K RKS ++ N      SV    +   +   G +   F  +N    A  GR+IGKLF++
Sbjct: 442 KKKNRKSAKDTNRQ----SVPRGQDQFELIEGGQMLLGF--NNFQSGATDGRKIGKLFLS 501

Query: 516 NKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDRHPNIVRWYGVES 575
           +KEIA+GSNGT+V EG+YEGRPVAVKRLV++HH+VAFKE+QNLIASD+H NI+RWYGVE 
Sbjct: 502 SKEIAKGSNGTVVFEGIYEGRPVAVKRLVRSHHEVAFKEIQNLIASDQHTNIIRWYGVEY 561

Query: 576 DQDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKL 635
           DQDFVYLSLERCTCSLDDLI+   + S   +L  +  TE +  Y++ L+S++ V+     
Sbjct: 562 DQDFVYLSLERCTCSLDDLIKSYLEFSMTKVLENNDSTEGVAAYKIQLDSLEGVIKGNNF 621

Query: 636 WKENGHPSSVLLKLMRDMVAGVEHLHELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKR 695
           WK  GHPS ++LKLMRD+V G+ HLHELGI+HRDLKPQNVLI K  ++ AKLSDMGISKR
Sbjct: 622 WKVGGHPSPLMLKLMRDIVCGIVHLHELGIVHRDLKPQNVLISKDMTLSAKLSDMGISKR 681

Query: 696 LLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPF 755
           +  DMSSLGH ATG+     GSSGWQAPEQLL GRQTRAVD+FSLGCV+F+ ITG +HPF
Sbjct: 682 MSRDMSSLGHLATGS-----GSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPF 741

Query: 756 GDRLERDVNIVKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRL 815
           GD LERDVNIVKN++DLFLV+ +PEA DLIS+LLNPDPDLRP A+EVL HP+FW+SE RL
Sbjct: 742 GDDLERDVNIVKNKVDLFLVEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRL 801

Query: 816 SFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSV 875
           SFLRD SDRVELE+RE++S +LKA+E TA +A+G KWDEK+EP+FITNIGRYRRYKYDS+
Sbjct: 802 SFLRDASDRVELENREADSEILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSI 840

Query: 876 RDLLRVMRNKLNHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEEC 932
           RDLLRV+RNKLNH+RELP EIQEL+G+VPEGFD YFA RFP+LLIEVY++IS +CR+EE 
Sbjct: 862 RDLLRVIRNKLNHHRELPPEIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEV 840

BLAST of MS010674 vs. TAIR 10
Match: AT5G24360.1 (inositol requiring 1-1 )

HSP 1 Score: 662.5 bits (1708), Expect = 4.9e-190
Identity = 403/884 (45.59%), Postives = 550/884 (62.22%), Query Frame = 0

Query: 68  LVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQA-HIEHDHNQENASGLSSSFFFDCGD 127
           LV+  DG+I LVD +S    W+F +  PIYSSYQA H  +  ++E +S L   F+ DC  
Sbjct: 50  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 109

Query: 128 DWELYMHTEHGKMKLPSSID--EVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNH 187
           DW LY  +     ++   +D  E +   PY   D  +++G + T+VF +D  TGKL++ +
Sbjct: 110 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 169

Query: 188 ISD--FSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVE-PRL-YITRTDYSLKSSFSG 247
             D  +S++ + N++  +    +  +   L   G     + P L YI R D+ ++   S 
Sbjct: 170 RMDELYSNTVVENDKEKAIVLSK--EAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CISK 229

Query: 248 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 307
             +V WS++ A++ A L   E       +  ++  N         PLS  + V + + R 
Sbjct: 230 FGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKN-------QFPLSYTTSVPMVQLR- 289

Query: 308 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPG---PAPNI 367
                +  Y+ L       + +L        P+QL + +       +L LPG       +
Sbjct: 290 -----NVKYETLFPRLGFLDEALYLPFQDRKPNQLAIGD-----GNQLTLPGNKEAEEVL 349

Query: 368 SSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTIL--VGLVTFSR 427
           S PLP    S+      +  I + S  Q       +G   L F F VT+L   GL  +  
Sbjct: 350 SLPLPETVISQ------ITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFFYRL 409

Query: 428 GLTGKVKQFFLREKLSSTSNSKILSSKKN---KARKSKRN--VNSDKRDISVSSENEDML 487
             + ++K+ ++ E   +T   K   SKKN   KA   K N  ++   +D S   ENE  L
Sbjct: 410 RQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVHKKENGFISGGNKDPS-HEENEKRL 469

Query: 488 MPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRL 547
           + +   +NN         ++  G R+GKLFV+NKEIA+GSNGT+VLEG YEGR VAVKRL
Sbjct: 470 LTAFPGLNN---------SSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 529

Query: 548 VKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSF 607
           V++HHDVA KE+ NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S    
Sbjct: 530 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS---- 589

Query: 608 NSMLGLDQDTEPMIKYRLHLESVKDVMPD---LKLWKENGHPSSVLLKLMRDMVAGVEHL 667
            ++L       PM    +H   +  +  +   ++LWKENGHPS VLLKLMRD+VAG+ HL
Sbjct: 590 -ALL-----ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHL 649

Query: 668 HELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGW 727
           H++GI+HRDLKPQNVLI+K  S+CAKLSDMGISKRL AD S+L  ++TG    G GSSGW
Sbjct: 650 HDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG---LGSGSSGW 709

Query: 728 QAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPE 787
           QAPEQL + RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERDVN++ +Q DLFL++S+PE
Sbjct: 710 QAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPE 769

Query: 788 AVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKAL 847
           AV L++ LLNPDP+LRP+A +V+ HPLFW+S+ RLSFLRD SDRVELE+RE  S LL AL
Sbjct: 770 AVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAAL 829

Query: 848 EKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELI 907
           E TA + L  +WDEK++ IF+ NIGRYRRYK+DS+RDLLRV+RNKLNHYRELP E+QEL+
Sbjct: 830 ESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELL 880

Query: 908 GSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHI 932
           GSVPEGF+ YF+SRFP+LLI+VY ++  YC  EE F KY K+ +
Sbjct: 890 GSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 880

BLAST of MS010674 vs. TAIR 10
Match: AT5G24360.2 (inositol requiring 1-1 )

HSP 1 Score: 662.5 bits (1708), Expect = 4.9e-190
Identity = 403/884 (45.59%), Postives = 550/884 (62.22%), Query Frame = 0

Query: 68  LVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQA-HIEHDHNQENASGLSSSFFFDCGD 127
           LV+  DG+I LVD +S    W+F +  PIYSSYQA H  +  ++E +S L   F+ DC  
Sbjct: 56  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 115

Query: 128 DWELYMHTEHGKMKLPSSID--EVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNH 187
           DW LY  +     ++   +D  E +   PY   D  +++G + T+VF +D  TGKL++ +
Sbjct: 116 DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 175

Query: 188 ISD--FSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVE-PRL-YITRTDYSLKSSFSG 247
             D  +S++ + N++  +    +  +   L   G     + P L YI R D+ ++   S 
Sbjct: 176 RMDELYSNTVVENDKEKAIVLSK--EAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CISK 235

Query: 248 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 307
             +V WS++ A++ A L   E       +  ++  N         PLS  + V + + R 
Sbjct: 236 FGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKN-------QFPLSYTTSVPMVQLR- 295

Query: 308 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPG---PAPNI 367
                +  Y+ L       + +L        P+QL + +       +L LPG       +
Sbjct: 296 -----NVKYETLFPRLGFLDEALYLPFQDRKPNQLAIGD-----GNQLTLPGNKEAEEVL 355

Query: 368 SSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTIL--VGLVTFSR 427
           S PLP    S+      +  I + S  Q       +G   L F F VT+L   GL  +  
Sbjct: 356 SLPLPETVISQ------ITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFFYRL 415

Query: 428 GLTGKVKQFFLREKLSSTSNSKILSSKKN---KARKSKRN--VNSDKRDISVSSENEDML 487
             + ++K+ ++ E   +T   K   SKKN   KA   K N  ++   +D S   ENE  L
Sbjct: 416 RQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVHKKENGFISGGNKDPS-HEENEKRL 475

Query: 488 MPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRL 547
           + +   +NN         ++  G R+GKLFV+NKEIA+GSNGT+VLEG YEGR VAVKRL
Sbjct: 476 LTAFPGLNN---------SSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 535

Query: 548 VKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSF 607
           V++HHDVA KE+ NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S    
Sbjct: 536 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS---- 595

Query: 608 NSMLGLDQDTEPMIKYRLHLESVKDVMPD---LKLWKENGHPSSVLLKLMRDMVAGVEHL 667
            ++L       PM    +H   +  +  +   ++LWKENGHPS VLLKLMRD+VAG+ HL
Sbjct: 596 -ALL-----ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHL 655

Query: 668 HELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGW 727
           H++GI+HRDLKPQNVLI+K  S+CAKLSDMGISKRL AD S+L  ++TG    G GSSGW
Sbjct: 656 HDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG---LGSGSSGW 715

Query: 728 QAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPE 787
           QAPEQL + RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERDVN++ +Q DLFL++S+PE
Sbjct: 716 QAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPE 775

Query: 788 AVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKAL 847
           AV L++ LLNPDP+LRP+A +V+ HPLFW+S+ RLSFLRD SDRVELE+RE  S LL AL
Sbjct: 776 AVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAAL 835

Query: 848 EKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELI 907
           E TA + L  +WDEK++ IF+ NIGRYRRYK+DS+RDLLRV+RNKLNHYRELP E+QEL+
Sbjct: 836 ESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELL 886

Query: 908 GSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHI 932
           GSVPEGF+ YF+SRFP+LLI+VY ++  YC  EE F KY K+ +
Sbjct: 896 GSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 886

BLAST of MS010674 vs. TAIR 10
Match: AT5G24360.3 (inositol requiring 1-1 )

HSP 1 Score: 662.5 bits (1708), Expect = 4.9e-190
Identity = 403/884 (45.59%), Postives = 550/884 (62.22%), Query Frame = 0

Query: 68  LVAASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQA-HIEHDHNQENASGLSSSFFFDCGD 127
           LV+  DG+I LVD +S    W+F +  PIYSSYQA H  +  ++E +S L   F+ DC  
Sbjct: 36  LVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFYMDCDK 95

Query: 128 DWELYMHTEHGKMKLPSSID--EVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNH 187
           DW LY  +     ++   +D  E +   PY   D  +++G + T+VF +D  TGKL++ +
Sbjct: 96  DWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTD-RIVLGKKDTSVFLLDWKTGKLVKRY 155

Query: 188 ISD--FSSSELSNEEHSSYNSKQNLDNKDLIQPGVVSPVE-PRL-YITRTDYSLKSSFSG 247
             D  +S++ + N++  +    +  +   L   G     + P L YI R D+ ++   S 
Sbjct: 156 RMDELYSNTVVENDKEKAIVLSK--EAPLLFGSGFKKSEDFPELVYIERKDFKIQ-CISK 215

Query: 248 SEEVAWSLNVAEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERS 307
             +V WS++ A++ A L   E       +  ++  N         PLS  + V + + R 
Sbjct: 216 FGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKN-------QFPLSYTTSVPMVQLR- 275

Query: 308 HVFSGSSGYKRLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPG---PAPNI 367
                +  Y+ L       + +L        P+QL + +       +L LPG       +
Sbjct: 276 -----NVKYETLFPRLGFLDEALYLPFQDRKPNQLAIGD-----GNQLTLPGNKEAEEVL 335

Query: 368 SSPLPPNSSSKAVIPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTIL--VGLVTFSR 427
           S PLP    S+      +  I + S  Q       +G   L F F VT+L   GL  +  
Sbjct: 336 SLPLPETVISQ------ITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFFYRL 395

Query: 428 GLTGKVKQFFLREKLSSTSNSKILSSKKN---KARKSKRN--VNSDKRDISVSSENEDML 487
             + ++K+ ++ E   +T   K   SKKN   KA   K N  ++   +D S   ENE  L
Sbjct: 396 RQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVHKKENGFISGGNKDPS-HEENEKRL 455

Query: 488 MPSEGNVNNWFHHSNLPDNAGTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRL 547
           + +   +NN         ++  G R+GKLFV+NKEIA+GSNGT+VLEG YEGR VAVKRL
Sbjct: 456 LTAFPGLNN---------SSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRL 515

Query: 548 VKTHHDVAFKEVQNLIASDRHPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSF 607
           V++HHDVA KE+ NL+ASD+H NIVRWYGV+ D+ F+Y+SLE C CSL+DLI   S    
Sbjct: 516 VQSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASS---- 575

Query: 608 NSMLGLDQDTEPMIKYRLHLESVKDVMPD---LKLWKENGHPSSVLLKLMRDMVAGVEHL 667
            ++L       PM    +H   +  +  +   ++LWKENGHPS VLLKLMRD+VAG+ HL
Sbjct: 576 -ALL-----ESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLMRDIVAGLVHL 635

Query: 668 HELGIIHRDLKPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHATGNTTAGCGSSGW 727
           H++GI+HRDLKPQNVLI+K  S+CAKLSDMGISKRL AD S+L  ++TG    G GSSGW
Sbjct: 636 HDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG---LGSGSSGW 695

Query: 728 QAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNQMDLFLVQSIPE 787
           QAPEQL + RQTRAVDLFSLGCVLFFC+TGG+HP+GD  ERDVN++ +Q DLFL++S+PE
Sbjct: 696 QAPEQLRNERQTRAVDLFSLGCVLFFCMTGGKHPYGDNYERDVNVLNDQKDLFLIESLPE 755

Query: 788 AVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVELEDRESESGLLKAL 847
           AV L++ LLNPDP+LRP+A +V+ HPLFW+S+ RLSFLRD SDRVELE+RE  S LL AL
Sbjct: 756 AVHLLTGLLNPDPNLRPRAQDVMHHPLFWNSDMRLSFLRDASDRVELENREEGSQLLAAL 815

Query: 848 EKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPNEIQELI 907
           E TA + L  +WDEK++ IF+ NIGRYRRYK+DS+RDLLRV+RNKLNHYRELP E+QEL+
Sbjct: 816 ESTAAVTLNGRWDEKLDSIFLDNIGRYRRYKFDSIRDLLRVIRNKLNHYRELPKELQELL 866

Query: 908 GSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHI 932
           GSVPEGF+ YF+SRFP+LLI+VY ++  YC  EE F KY K+ +
Sbjct: 876 GSVPEGFERYFSSRFPKLLIQVYTVLFDYCNNEEFFFKYSKTTV 866

BLAST of MS010674 vs. TAIR 10
Match: AT3G11870.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 322.4 bits (825), Expect = 1.2e-87
Identity = 221/566 (39.05%), Postives = 319/566 (56.36%), Query Frame = 0

Query: 403 LVTILVGLVTFS--RGLTGKVKQFFLREKLSSTSNSKILSSKKNKARKSKRNVNSD---- 462
           LV ++   + FS  +GL G V +    +K    S  ++ +S ++  +  +    SD    
Sbjct: 12  LVVVVCVFLRFSKDKGLDGIVNE----KKRDKNSAPRVSASGEDGTKNEQVEKKSDPSGG 71

Query: 463 --KRDISVSSENEDMLMPSEGNVNNW-------FHHSNLPDNAGTGRRIG-KLFVTNKEI 522
             + +   +SE++ + +PS+ N+N                +N   G+ +  +L V+  E+
Sbjct: 72  LGEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRRLLVSTNEM 131

Query: 523 ARGSNGTIVLEGVYEGR-PVAVKRLVKTHHDVAF--KEVQNLIASDRHPNIVRWYGVESD 582
             G NG  V +GVY  R  VAVK L   H   AF   E+ N    D H NI+R++G+E D
Sbjct: 132 KYGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNIIRFHGLEQD 191

Query: 583 QDFVYLSLERCTCSLDDLIQICSDQSFNSMLGLDQDTEPMIKYRLHLESVKDVMPDLKLW 642
           Q F Y+ LE   CSLDDLI++       S+    +DT+ +      ++ ++ VM  +K W
Sbjct: 192 QSFAYICLEPWKCSLDDLIKL-------SVRRTKRDTQAVAP----VDDLEKVMKRIKFW 251

Query: 643 KENGHPSSV--LLKLMRDMVAGVEHLHELGIIHRDLKPQNVLII-KQKSMCAKLSDMGIS 702
           KE G P  +  +LKLMRD+V G+ HLH+L  IHR+L PQNVLII K  ++ AK+SDM +S
Sbjct: 252 KEKGKPLPLTPMLKLMRDVVCGLAHLHKLKTIHRNLNPQNVLIIVKDMTLTAKISDMSLS 311

Query: 703 KRLLADMSSLGHHATGNTTAGCGSSGWQAPEQLLHGRQTR---AVDLFSLGCVLFFCITG 762
           K L    SS  H AT +     GSSGWQAPEQL   ++ +     D+F+ GC+L + +  
Sbjct: 312 KHLGGKKSSYKHLATCS-----GSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM- 371

Query: 763 GRHPFGDRLERDVNI-VKNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFW 822
           G HPFG   ERD NI   N+ +L LV ++ EA++LI QLLN  PDLRP A++VL HPLFW
Sbjct: 372 GTHPFGSPSERDTNIKTNNKTNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFW 431

Query: 823 SSETRLSFLRDTSDRVELEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRY-- 882
            SE RL FLR+ SDR+EL+   +  G L        L     W  K+   FIT+I     
Sbjct: 432 DSEKRLFFLREASDRIELD--ITMWGDLNKTIAPRVLGESKDWASKLGKTFITHIENLAQ 491

Query: 883 -----------RRYKYDSVRDLLRVMRNKLNHYRELPNE--IQELIGSVPEGFDGYFASR 928
                      R YKY S+R LLR++RN L+H+RE+ ++  I+E++G VPEG D +F +R
Sbjct: 492 AQPGQESRQYNRSYKYWSLRHLLRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTAR 551