MS010658 (gene) Bitter gourd (TR) v1

Overview


Name	MS010658
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Chitinase
Location	scaffold35: 1226420 .. 1227555 (-)
RNA-Seq Expression	MS010658
Synteny	MS010658

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGAAAATGAAAGCCCATACGCTAATCATCTTGGCCTTTGCCTTGTTTTTGGGAGCTGCTTCTGCGGAGCAATGTGGGCGGCAGGCCAATGGCGCCCTCTGCCCCAATCGCCTCTGCTGCAGCCGGTTCGGGTGGTGCGGCTCCACTGATGAGTACTGCAAGGATGGTTGCCAGAGCCAGTGTGGCGACCAAACTCCTACACCCTCAAACCCGGGATCGGGCGACGTCGGTAGCATCATCACCTCTGCCATTTTCGACCAAATGCTCAAGTATCGCAACGATGCGCGATGCCCTAGTAATGGGTTCTATAGTTACAATGCTTTCATTTCTGCTGCGAGATCCTTTCCTGGCTTTGGAACAACAGGAGATGATGCTACCCGTAAAAGGGAGTTGGCGGCTTTCTTGGGCCAAACTTCTCATGAAACTACTGGTTACTACTTTGAACTTGAAGTCTCTGTTAAAATAGATTAGTGTGAAGTGATGCACCTTATTTGAATATTATACAATTGAATGTGATGTAGGTGGATGGGCAACTGCACCTGATGGTCCATATGCATGGGGATATTGTTTCATACGGGAGAGAAATCAAGATGTCTATTGCACACCCAGCCAACAATGGCCATGTGCTCCTGGTCAGAAATACTACGGTCGTGGACCAATCCAGCTTACTCAGTAAGTTCTATTTACTCTTTTGAATTTTTTTTTTTTAAGTTTATAATAAAATTTGTGAGAGATTGTAATCTTAATCCATAAAATTATTGAATTTTTTTAGTAACTACAACTACGGGCCAGCGGGAACAGCATTGGGATTGAATTTGTTGAATAATCCTGACTCTGTAGCCACAGATGCAACTGTATCATTTAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCAAGTCATGATGTTATTATTGGCAAGTGGCAACCGTCGAGTGCCGACAACTCTGCTGGAAGAGTTCCTGGCTATGGTGTCATCACAAACATCATTAATGGTGGACTCGAATGTGGGCATGGTCCCGATGACAGAGTTGCTGACAGAATTGGATTTTACAAAAGATACTGTGACATATTGAATACAAGTTACGGAAACAACTTAGATTGCTACAACCAAAGTCCGTTT

mRNA sequence

AAGAAAATGAAAGCCCATACGCTAATCATCTTGGCCTTTGCCTTGTTTTTGGGAGCTGCTTCTGCGGAGCAATGTGGGCGGCAGGCCAATGGCGCCCTCTGCCCCAATCGCCTCTGCTGCAGCCGGTTCGGGTGGTGCGGCTCCACTGATGAGTACTGCAAGGATGGTTGCCAGAGCCAGTGTGGCGACCAAACTCCTACACCCTCAAACCCGGGATCGGGCGACGTCGGTAGCATCATCACCTCTGCCATTTTCGACCAAATGCTCAAGTATCGCAACGATGCGCGATGCCCTAGTAATGGGTTCTATAGTTACAATGCTTTCATTTCTGCTGCGAGATCCTTTCCTGGCTTTGGAACAACAGGAGATGATGCTACCCGTAAAAGGGAGTTGGCGGCTTTCTTGGGCCAAACTTCTCATGAAACTACTGGTGGATGGGCAACTGCACCTGATGGTCCATATGCATGGGGATATTGTTTCATACGGGAGAGAAATCAAGATGTCTATTGCACACCCAGCCAACAATGGCCATGTGCTCCTGGTCAGAAATACTACGGTCGTGGACCAATCCAGCTTACTCATAACTACAACTACGGGCCAGCGGGAACAGCATTGGGATTGAATTTGTTGAATAATCCTGACTCTGTAGCCACAGATGCAACTGTATCATTTAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCAAGTCATGATGTTATTATTGGCAAGTGGCAACCGTCGAGTGCCGACAACTCTGCTGGAAGAGTTCCTGGCTATGGTGTCATCACAAACATCATTAATGGTGGACTCGAATGTGGGCATGGTCCCGATGACAGAGTTGCTGACAGAATTGGATTTTACAAAAGATACTGTGACATATTGAATACAAGTTACGGAAACAACTTAGATTGCTACAACCAAAGTCCGTTT

Coding sequence (CDS)

AAGAAAATGAAAGCCCATACGCTAATCATCTTGGCCTTTGCCTTGTTTTTGGGAGCTGCTTCTGCGGAGCAATGTGGGCGGCAGGCCAATGGCGCCCTCTGCCCCAATCGCCTCTGCTGCAGCCGGTTCGGGTGGTGCGGCTCCACTGATGAGTACTGCAAGGATGGTTGCCAGAGCCAGTGTGGCGACCAAACTCCTACACCCTCAAACCCGGGATCGGGCGACGTCGGTAGCATCATCACCTCTGCCATTTTCGACCAAATGCTCAAGTATCGCAACGATGCGCGATGCCCTAGTAATGGGTTCTATAGTTACAATGCTTTCATTTCTGCTGCGAGATCCTTTCCTGGCTTTGGAACAACAGGAGATGATGCTACCCGTAAAAGGGAGTTGGCGGCTTTCTTGGGCCAAACTTCTCATGAAACTACTGGTGGATGGGCAACTGCACCTGATGGTCCATATGCATGGGGATATTGTTTCATACGGGAGAGAAATCAAGATGTCTATTGCACACCCAGCCAACAATGGCCATGTGCTCCTGGTCAGAAATACTACGGTCGTGGACCAATCCAGCTTACTCATAACTACAACTACGGGCCAGCGGGAACAGCATTGGGATTGAATTTGTTGAATAATCCTGACTCTGTAGCCACAGATGCAACTGTATCATTTAAGACAGCCATTTGGTTTTGGATGACACCACAAGGAAACAAACCATCAAGTCATGATGTTATTATTGGCAAGTGGCAACCGTCGAGTGCCGACAACTCTGCTGGAAGAGTTCCTGGCTATGGTGTCATCACAAACATCATTAATGGTGGACTCGAATGTGGGCATGGTCCCGATGACAGAGTTGCTGACAGAATTGGATTTTACAAAAGATACTGTGACATATTGAATACAAGTTACGGAAACAACTTAGATTGCTACAACCAAAGTCCGTTT

Protein sequence

KKMKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCGDQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTSYGNNLDCYNQSPF

Homology

[Close]

BLAST of MS010658 vs. NCBI nr
Match: XP_022132794.1 (endochitinase A-like [Momordica charantia])

HSP 1 Score: 661.4 bits (1705), Expect = 4.0e-186
Identity = 304/313 (97.12%), Postives = 307/313 (98.08%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCS+ GWCGSTDEYCK+GCQSQCG
Sbjct: 1   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQCG 60

Query: 63  DQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 122
            QTP P+NPGSGDVG IIT AIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG
Sbjct: 61  GQTPPPTNPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 120

Query: 123 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 182
           DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ
Sbjct: 121 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 180

Query: 183 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSH 242
           KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSV TDATVSFKTAIWFWMTPQGNKPSSH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVVTDATVSFKTAIWFWMTPQGNKPSSH 240

Query: 243 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 302
           DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS
Sbjct: 241 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 300

Query: 303 YGNNLDCYNQSPF 316
           YGNNLDCYNQSPF
Sbjct: 301 YGNNLDCYNQSPF 313

[Close]

BLAST of MS010658 vs. NCBI nr
Match: ABD66068.1 (chitinase [Momordica charantia])

HSP 1 Score: 627.1 bits (1616), Expect = 8.3e-176
Identity = 286/313 (91.37%), Postives = 296/313 (94.57%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCS+ GWCGSTDEYCK+GCQSQCG
Sbjct: 1   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQCG 60

Query: 63  DQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 122
            QTPTP+NPGSGDVG IIT AIFDQMLKYRNDARCPSNGFYSYNAFISA RSFPGFGTTG
Sbjct: 61  GQTPTPTNPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISATRSFPGFGTTG 120

Query: 123 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 182
           DDATRKRELAAFLGQTSHETTGGW +APDGP+AWGYCFIRERNQD YC+P+QQWPCAPGQ
Sbjct: 121 DDATRKRELAAFLGQTSHETTGGWPSAPDGPFAWGYCFIRERNQDTYCSPNQQWPCAPGQ 180

Query: 183 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSH 242
           KYYGRGPIQLTHNYNYGPAG ALGLNLLNNPDSVATD TVSFKTAIWFWMTPQGNKPSSH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGRALGLNLLNNPDSVATDPTVSFKTAIWFWMTPQGNKPSSH 240

Query: 243 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 302
           DVIIGKWQPS AD++AGRVPGYGVITNIINGGLECG GPD RVADRIGF+KRYCDIL   
Sbjct: 241 DVIIGKWQPSGADSAAGRVPGYGVITNIINGGLECGRGPDSRVADRIGFFKRYCDILRIG 300

Query: 303 YGNNLDCYNQSPF 316
           YGNNLDC NQ PF
Sbjct: 301 YGNNLDCNNQRPF 313

[Close]

BLAST of MS010658 vs. NCBI nr
Match: XP_022949791.1 (endochitinase-like [Cucurbita moschata])

HSP 1 Score: 567.8 bits (1462), Expect = 5.9e-158
Identity = 260/314 (82.80%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK HTLI+LAFA  LG A AEQCGRQANGALCPN LCCS+FGWCG TDEYCKDGCQSQCG
Sbjct: 8   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 63  DQTP-TPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTT 122
             TP TP + G   VGSII+ ++++QMLKY  D RCPSNGFY YNAF++AA+SFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 123 GDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPG 182
           GD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYC+P+QQWPCA G
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 183 QKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSS 242
           QKYYGRGPIQLTHNYNYGPAG AL LNLL NPD VATDA VSFKTAIWFWMTPQGNKPS 
Sbjct: 188 QKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 247

Query: 243 HDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNT 302
           HDVI G+WQPSSAD++AGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRY D+L  
Sbjct: 248 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 307

Query: 303 SYGNNLDCYNQSPF 316
           SYG+NLDC NQ  F
Sbjct: 308 SYGSNLDCNNQRSF 320

[Close]

BLAST of MS010658 vs. NCBI nr
Match: XP_023544980.1 (endochitinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 565.5 bits (1456), Expect = 3.0e-157
Identity = 259/314 (82.48%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK HTLI+LAFA  LG A AEQCGRQANGALCPN LCCS+FGWCG TDEYCKDGCQSQCG
Sbjct: 1   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 60

Query: 63  DQTP-TPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTT 122
             TP TP + G   VGSII+ A+++QMLKY  D RCPSNGFY YNAF++AA+SFPGFGTT
Sbjct: 61  GSTPSTPPSSGGSGVGSIISEALYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 120

Query: 123 GDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPG 182
           GD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYC+P+QQWPCA G
Sbjct: 121 GDTATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 180

Query: 183 QKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSS 242
           QKYYGRGPIQLTHNYNYGPAG AL LNLL+NPD VATDA V+FKTAIWFWMTPQGNKPS 
Sbjct: 181 QKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVAFKTAIWFWMTPQGNKPSC 240

Query: 243 HDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNT 302
           HDVI G+WQPSSAD++AGRV GYGVITNIINGGLECGHGPDDRVADRIGFYKRY D+L  
Sbjct: 241 HDVITGRWQPSSADSAAGRVAGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 300

Query: 303 SYGNNLDCYNQSPF 316
           SYG+NLDC NQ  F
Sbjct: 301 SYGSNLDCNNQRSF 313

[Close]

BLAST of MS010658 vs. NCBI nr
Match: XP_022977305.1 (chitinase 1-like [Cucurbita maxima])

HSP 1 Score: 563.9 bits (1452), Expect = 8.6e-157
Identity = 260/314 (82.80%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK  TLI+LAFA  LGAA AEQCGRQANGALCPN LCCS+FGWCG TDEYCK+GCQSQCG
Sbjct: 1   MKLRTLIVLAFAFVLGAA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCG 60

Query: 63  DQTP-TPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTT 122
             TP TP + G G VGSII+ A+++QMLKY  D RCPSNGFY YNAFI+A +SFPGFGTT
Sbjct: 61  GTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120

Query: 123 GDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPG 182
           GD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYC+P+QQWPCA G
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180

Query: 183 QKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSS 242
           QKYYGRGPIQLTHNYNYGPAG AL LNLL+NPD VATDA VSFKTAIWFWMTPQGNKPS 
Sbjct: 181 QKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 240

Query: 243 HDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNT 302
           HDVI G+WQPSSAD++AGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRY D+L  
Sbjct: 241 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 300

Query: 303 SYGNNLDCYNQSPF 316
           SYG+NLDC NQ  F
Sbjct: 301 SYGSNLDCNNQRSF 313

[Close]

BLAST of MS010658 vs. ExPASy Swiss-Prot
Match: P36907 (Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 3.9e-136
Identity = 224/320 (70.00%), Postives = 261/320 (81.56%), Query Frame = 0


Query: 1   KKMKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQ 60
           + +K    I+    LFLG + AEQCG QA GA+CPN LCCS+FG+CGSTD YC DGCQSQ
Sbjct: 3   RTLKVSFFILCLLPLFLG-SKAEQCGSQAGGAVCPNGLCCSKFGFCGSTDPYCGDGCQSQ 62

Query: 61  CGDQTPTPSNP----GSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFP 120
           C   +PTP+ P    G GDVG ++ S++FDQMLKYRND RC  +GFY+Y+AFI+AARSF 
Sbjct: 63  C-KSSPTPTIPTPSTGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIAAARSFN 122

Query: 121 GFGTTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERN-QDVYCTPSQQ 180
           GFGTTGDD T+K+ELAAFL QTSHETTGGW TAPDGPYAWGYCF+ E+N Q+VYC+P + 
Sbjct: 123 GFGTTGDDNTKKKELAAFLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSP-KD 182

Query: 181 WPCAPGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQ 240
           WPCAPG+KYYGRGPIQLTHNYNYG AG A+  +L+NNPD ++T+ TVSFKTAIWFWMTPQ
Sbjct: 183 WPCAPGKKYYGRGPIQLTHNYNYGLAGQAIKEDLINNPDLLSTNPTVSFKTAIWFWMTPQ 242

Query: 241 GNKPSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRY 300
            NKPSSHDVI G+W PS+AD+SAGRVPGYGVITNIINGG+ECGHG D+RV DR+GFYKRY
Sbjct: 243 ANKPSSHDVITGRWTPSAADSSAGRVPGYGVITNIINGGIECGHGQDNRVDDRVGFYKRY 302

Query: 301 CDILNTSYGNNLDCYNQSPF 316
           C I     G NLDC NQ  F
Sbjct: 303 CQIFGVDPGGNLDCNNQRSF 319

[Close]

BLAST of MS010658 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 481.1 bits (1237), Expect = 9.6e-135
Identity = 214/309 (69.26%), Postives = 254/309 (82.20%), Query Frame = 0


Query: 8   LIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCGDQTPT 67
           L++L   L  G A AEQCGRQA GALCP  LCCS+FGWCGST +YC   CQSQCG  TP+
Sbjct: 11  LLLLLVGLLAGEAFAEQCGRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGGVTPS 70

Query: 68  PSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTGDDATR 127
           P     G V S+I+ ++F+QMLK+RNDA C + GFY+YNAFI+AA SF GF + GD ATR
Sbjct: 71  P----GGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTATR 130

Query: 128 KRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRER-NQDVYCTPSQQWPCAPGQKYYG 187
           KRE+AAFL QTSHETTGGWATAPDGPYAWGYCF++E+ N   YC P+ QWPCAPG+KYYG
Sbjct: 131 KREIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYYG 190

Query: 188 RGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSHDVII 247
           RGPIQ+++NYNYGPAG A+G +L+NNPD+VATD  +SFKTA+WFWMTPQ  KPS H+VI 
Sbjct: 191 RGPIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCHNVIT 250

Query: 248 GKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTSYGNN 307
           G+W PS+AD +AGR+PGYGVITNIINGG+ECG G +D+VADRIGFYKRYCD+L  SYG+N
Sbjct: 251 GRWTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVSYGSN 310

Query: 308 LDCYNQSPF 316
           LDCYNQ  F
Sbjct: 311 LDCYNQRSF 315

[Close]

BLAST of MS010658 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 479.6 bits (1233), Expect = 2.8e-134
Identity = 221/316 (69.94%), Postives = 258/316 (81.65%), Query Frame = 0


Query: 4   KAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDG-CQSQC- 63
           K   L  L F+L L +ASAEQCG QA GA CP+ LCCS+FGWCG+T++YC  G CQSQC 
Sbjct: 5   KFTALSSLLFSLLLLSASAEQCGSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQCP 64

Query: 64  GDQTPT-PSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGT 123
           G  TPT P+ PG GD+GSII+S++FDQMLK+RND  C   GFYSYNAFI+AARSFPGFGT
Sbjct: 65  GGPTPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGT 124

Query: 124 TGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRER-NQDVYCTPSQQWPCA 183
           +GD   RKRE+AAF  QTSHETTGGWATAPDGPYAWGYC++RE+ +   YCTPS QWPCA
Sbjct: 125 SGDTTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCA 184

Query: 184 PGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKP 243
           PG+KY+GRGPIQ++HNYNYGP G A+G++LLNNPD VATD  +SFK+A+WFWMTPQ  KP
Sbjct: 185 PGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKP 244

Query: 244 SSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDIL 303
           S HDVIIG+WQPS+ D +A R+PG+GVITNIINGGLECG G D RV DRIGFY+RYC IL
Sbjct: 245 SCHDVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSIL 304

Query: 304 NTSYGNNLDCYNQSPF 316
             S G+NLDC NQ  F
Sbjct: 305 GVSPGDNLDCGNQRSF 320

[Close]

BLAST of MS010658 vs. ExPASy Swiss-Prot
Match: Q9FRV1 (Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.6e-134
Identity = 221/318 (69.50%), Postives = 249/318 (78.30%), Query Frame = 0


Query: 12  AFALF------LGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG--- 71
           AFALF      +  A AEQCG QA GA CPN LCCSRFGWCGST +YC DGCQSQC    
Sbjct: 3   AFALFAVLAMAVTMAVAEQCGSQAGGATCPNCLCCSRFGWCGSTSDYCGDGCQSQCAGCG 62

Query: 72  ----DQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGF 131
                 TPTP+  G G V SI++ A+FD+ML +RND  C + GFY+Y+AF++AA +FPGF
Sbjct: 63  GGGTPVTPTPTPSGGGGVSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAAGAFPGF 122

Query: 132 GTTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERN-QDVYCTPSQQWP 191
           GTTG   TRKRE+AAFL QTSHETTGGWATAPDG +AWGYCF +ER     YCTPS QWP
Sbjct: 123 GTTGSTDTRKREVAAFLAQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPSAQWP 182

Query: 192 CAPGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGN 251
           CAPG+ YYGRGPIQL+HNYNYGPAG A+G++LL NPD VATD TVSFKTA+WFWMT Q  
Sbjct: 183 CAPGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAP 242

Query: 252 KPSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCD 311
           KPSSH VI G+W PS  D +AGRVPG+GVITNI+NGG+ECGHG D RVADRIGFYKRYCD
Sbjct: 243 KPSSHAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCD 302

Query: 312 ILNTSYGNNLDCYNQSPF 316
           IL   YGNNLDCYNQ PF
Sbjct: 303 ILRVGYGNNLDCYNQRPF 320

[Close]

BLAST of MS010658 vs. ExPASy Swiss-Prot
Match: P24091 (Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 1.5e-132
Identity = 218/307 (71.01%), Postives = 253/307 (82.41%), Query Frame = 0


Query: 11  LAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDG-CQSQCGDQTPTPS 70
           L F+L L +ASAEQCG QA GA C + LCCS+FGWCG+T++YC  G CQSQC    PTP 
Sbjct: 12  LLFSLLLLSASAEQCGSQAGGARCASGLCCSKFGWCGNTNDYCGPGNCQSQC-PGGPTP- 71

Query: 71  NPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTGDDATRKR 130
            PG GD+GSII+S++FDQMLK+RND  C   GFYSYNAFI+AARSFPGFGT+GD   RKR
Sbjct: 72  -PGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKR 131

Query: 131 ELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRER-NQDVYCTPSQQWPCAPGQKYYGRG 190
           E+AAF  QTSHETTGGWATAPDGPYAWGYC++RE+ +   YCTPS QWPCAPG+KY+GRG
Sbjct: 132 EIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRG 191

Query: 191 PIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSHDVIIGK 250
           PIQ++HNYNYGP G A+G++LLNNPD VATD  +SFK+A+WFWMTPQ  KPS HDVIIG+
Sbjct: 192 PIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGR 251

Query: 251 WQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTSYGNNLD 310
           WQPSSAD +A R+PG+GVITNIINGGLECG G D RV DRIGFY+RYC IL  S G+NLD
Sbjct: 252 WQPSSADRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVSPGDNLD 311

Query: 311 CYNQSPF 316
           C NQ  F
Sbjct: 312 CGNQRSF 315

[Close]

BLAST of MS010658 vs. ExPASy TrEMBL
Match: A0A6J1BU42 (endochitinase A-like OS=Momordica charantia OX=3673 GN=LOC111005566 PE=4 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 1.9e-186
Identity = 304/313 (97.12%), Postives = 307/313 (98.08%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCS+ GWCGSTDEYCK+GCQSQCG
Sbjct: 1   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQCG 60

Query: 63  DQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 122
            QTP P+NPGSGDVG IIT AIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG
Sbjct: 61  GQTPPPTNPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 120

Query: 123 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 182
           DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ
Sbjct: 121 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 180

Query: 183 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSH 242
           KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSV TDATVSFKTAIWFWMTPQGNKPSSH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVVTDATVSFKTAIWFWMTPQGNKPSSH 240

Query: 243 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 302
           DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS
Sbjct: 241 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 300

Query: 303 YGNNLDCYNQSPF 316
           YGNNLDCYNQSPF
Sbjct: 301 YGNNLDCYNQSPF 313

[Close]

BLAST of MS010658 vs. ExPASy TrEMBL
Match: Q207U1 (Chitinase OS=Momordica charantia OX=3673 GN=ChiA PE=4 SV=1)

HSP 1 Score: 627.1 bits (1616), Expect = 4.0e-176
Identity = 286/313 (91.37%), Postives = 296/313 (94.57%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCS+ GWCGSTDEYCK+GCQSQCG
Sbjct: 1   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSQHGWCGSTDEYCKNGCQSQCG 60

Query: 63  DQTPTPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTG 122
            QTPTP+NPGSGDVG IIT AIFDQMLKYRNDARCPSNGFYSYNAFISA RSFPGFGTTG
Sbjct: 61  GQTPTPTNPGSGDVGRIITPAIFDQMLKYRNDARCPSNGFYSYNAFISATRSFPGFGTTG 120

Query: 123 DDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPGQ 182
           DDATRKRELAAFLGQTSHETTGGW +APDGP+AWGYCFIRERNQD YC+P+QQWPCAPGQ
Sbjct: 121 DDATRKRELAAFLGQTSHETTGGWPSAPDGPFAWGYCFIRERNQDTYCSPNQQWPCAPGQ 180

Query: 183 KYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSH 242
           KYYGRGPIQLTHNYNYGPAG ALGLNLLNNPDSVATD TVSFKTAIWFWMTPQGNKPSSH
Sbjct: 181 KYYGRGPIQLTHNYNYGPAGRALGLNLLNNPDSVATDPTVSFKTAIWFWMTPQGNKPSSH 240

Query: 243 DVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTS 302
           DVIIGKWQPS AD++AGRVPGYGVITNIINGGLECG GPD RVADRIGF+KRYCDIL   
Sbjct: 241 DVIIGKWQPSGADSAAGRVPGYGVITNIINGGLECGRGPDSRVADRIGFFKRYCDILRIG 300

Query: 303 YGNNLDCYNQSPF 316
           YGNNLDC NQ PF
Sbjct: 301 YGNNLDCNNQRPF 313

[Close]

BLAST of MS010658 vs. ExPASy TrEMBL
Match: A0A6J1GD14 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 2.9e-158
Identity = 260/314 (82.80%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK HTLI+LAFA  LG A AEQCGRQANGALCPN LCCS+FGWCG TDEYCKDGCQSQCG
Sbjct: 8   MKLHTLIVLAFAFVLGGA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKDGCQSQCG 67

Query: 63  DQTP-TPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTT 122
             TP TP + G   VGSII+ ++++QMLKY  D RCPSNGFY YNAF++AA+SFPGFGTT
Sbjct: 68  GSTPSTPPSSGGSGVGSIISESLYNQMLKYSKDPRCPSNGFYRYNAFVTAAQSFPGFGTT 127

Query: 123 GDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPG 182
           GD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYC+P+QQWPCA G
Sbjct: 128 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCAAG 187

Query: 183 QKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSS 242
           QKYYGRGPIQLTHNYNYGPAG AL LNLL NPD VATDA VSFKTAIWFWMTPQGNKPS 
Sbjct: 188 QKYYGRGPIQLTHNYNYGPAGNALNLNLLGNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 247

Query: 243 HDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNT 302
           HDVI G+WQPSSAD++AGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRY D+L  
Sbjct: 248 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 307

Query: 303 SYGNNLDCYNQSPF 316
           SYG+NLDC NQ  F
Sbjct: 308 SYGSNLDCNNQRSF 320

[Close]

BLAST of MS010658 vs. ExPASy TrEMBL
Match: A0A6J1ILX6 (chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1)

HSP 1 Score: 563.9 bits (1452), Expect = 4.2e-157
Identity = 260/314 (82.80%), Postives = 278/314 (88.54%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK  TLI+LAFA  LGAA AEQCGRQANGALCPN LCCS+FGWCG TDEYCK+GCQSQCG
Sbjct: 1   MKLRTLIVLAFAFVLGAA-AEQCGRQANGALCPNGLCCSQFGWCGDTDEYCKNGCQSQCG 60

Query: 63  DQTP-TPSNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTT 122
             TP TP + G G VGSII+ A+++QMLKY  D RCPSNGFY YNAFI+A +SFPGFGTT
Sbjct: 61  GTTPSTPPSSGGGGVGSIISEALYNQMLKYSKDPRCPSNGFYQYNAFIAATQSFPGFGTT 120

Query: 123 GDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCAPG 182
           GD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRE NQDVYC+P+QQWPCA G
Sbjct: 121 GDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIREINQDVYCSPNQQWPCAAG 180

Query: 183 QKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSS 242
           QKYYGRGPIQLTHNYNYGPAG AL LNLL+NPD VATDA VSFKTAIWFWMTPQGNKPS 
Sbjct: 181 QKYYGRGPIQLTHNYNYGPAGNALNLNLLSNPDLVATDAVVSFKTAIWFWMTPQGNKPSC 240

Query: 243 HDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNT 302
           HDVI G+WQPSSAD++AGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRY D+L  
Sbjct: 241 HDVITGRWQPSSADSAAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYSDMLGV 300

Query: 303 SYGNNLDCYNQSPF 316
           SYG+NLDC NQ  F
Sbjct: 301 SYGSNLDCNNQRSF 313

[Close]

BLAST of MS010658 vs. ExPASy TrEMBL
Match: A0A6J1GD34 (endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.7e-155
Identity = 256/316 (81.01%), Postives = 275/316 (87.03%), Query Frame = 0


Query: 3   MKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQSQCG 62
           MK H LI+LAFA  LGAA AEQCGRQANGALCPNRLCCS+FGWCG+TDEYCK+ CQSQCG
Sbjct: 1   MKLHALIVLAFAFVLGAA-AEQCGRQANGALCPNRLCCSQFGWCGNTDEYCKNNCQSQCG 60

Query: 63  DQTPTP---SNPGSGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFG 122
             TP+P   +  G G VGSII  A+++QMLKY  D RCPSNGFY YNAFI+AA+SFP FG
Sbjct: 61  GSTPSPPPSTGGGGGSVGSIINEALYNQMLKYSKDPRCPSNGFYRYNAFITAAQSFPAFG 120

Query: 123 TTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPSQQWPCA 182
           TTGD ATRKRELAAF GQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYC+P+QQWPCA
Sbjct: 121 TTGDAATRKRELAAFFGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCSPNQQWPCA 180

Query: 183 PGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKP 242
            GQKYYGRGPIQLTHNYNYGPAG AL LNLL NPD VATDA V+FKTAIWFWMTPQGNKP
Sbjct: 181 AGQKYYGRGPIQLTHNYNYGPAGRALNLNLLGNPDLVATDAVVAFKTAIWFWMTPQGNKP 240

Query: 243 SSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDIL 302
           S HDVI G+WQPS+AD++AGRVPGYGVITNIINGGLECG G D RVADRIGFYKRYCD+L
Sbjct: 241 SCHDVITGRWQPSAADSAAGRVPGYGVITNIINGGLECGRGADSRVADRIGFYKRYCDLL 300

Query: 303 NTSYGNNLDCYNQSPF 316
              YGNNLDC NQ  F
Sbjct: 301 GIGYGNNLDCNNQRSF 315

[Close]

BLAST of MS010658 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-132
Identity = 217/317 (68.45%), Postives = 253/317 (79.81%), Query Frame = 0


Query: 2   KMKAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKD-GCQSQ 61
           KMK +  + L F+L L  +SAEQCGRQA GALCPN LCCS FGWCG+T+ YCK  GCQSQ
Sbjct: 13  KMKTNLFLFLIFSLLLSLSSAEQCGRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQ 72

Query: 62  CGDQTPTPSNPG-SGDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFG 121
           C   TP  + PG +GD+  II+S+ FD MLK+RNDA CP+ GFY+YNAFI+AA+SFPGFG
Sbjct: 73  C---TPGGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFG 132

Query: 122 TTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERN-QDVYCTPSQQWPC 181
           TTGD ATRK+E+AAF GQTSHETTGGWATAPDGPY+WGYCF +E+N    YC PS  WPC
Sbjct: 133 TTGDTATRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPC 192

Query: 182 APGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNK 241
           A G++YYGRGP+QL+ NYNYG  G A+G++LLNNPD VA DA ++FK AIWFWMT Q  K
Sbjct: 193 ASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPK 252

Query: 242 PSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDI 301
           PS H VI G+WQPS AD +AGR+PGYGVITNIINGGLECG G D RVADRIGFY+RYC+I
Sbjct: 253 PSCHAVIAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNI 312

Query: 302 LNTSYGNNLDCYNQSPF 316
              + G NLDCYNQ  F
Sbjct: 313 FGVNPGGNLDCYNQRSF 326

[Close]

BLAST of MS010658 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.3e-81
Identity = 131/239 (54.81%), Postives = 176/239 (73.64%), Query Frame = 0


Query: 79  IITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTGDDATRKRELAAFLGQT 138
           ++   +++++  ++++  CP+NGFY+Y +F+ A R FP FG+ G   T++ E+AAFL Q 
Sbjct: 33  LVPRDLYNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAFLAQI 92

Query: 139 SHETTGGWATAPDGPYAWGYCFIRERN-QDVYCTPSQ-QWPCAPGQKYYGRGPIQLTHNY 198
           SHETTGGWATAPDGPYAWG CF  E + Q  YC  S  QWPC P + Y GRGPIQL+ NY
Sbjct: 93  SHETTGGWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQLSWNY 152

Query: 199 NYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSHDVIIGKWQPSSADN 258
           NYGPAG ALG + L NP++V+ ++ ++F+TA+WFWMTPQ  KPS HDV+IGK++P++AD 
Sbjct: 153 NYGPAGRALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRPTAADL 212

Query: 259 SAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTSYGNNLDCYNQSPF 316
           +A R  G+G+ TNIINGGLECG   D RV DRIGF++RY  +   + G NLDC NQ P+
Sbjct: 213 AANRTGGFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCENQRPY 271

[Close]

BLAST of MS010658 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.4e-80
Identity = 132/240 (55.00%), Postives = 174/240 (72.50%), Query Frame = 0


Query: 78  SIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAARSFPGFGTTGDDATRKRELAAFLGQ 137
           S++   ++DQ+  ++++  CP+ GFY Y AF+ A RSFP FG+ G+  TR+RE+AAFL Q
Sbjct: 40  SLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFLAQ 99

Query: 138 TSHETTGGWATAPDGPYAWGYCFIRERN-QDVYCTPS-QQWPCAPGQKYYGRGPIQLTHN 197
            SHETTGGWATAPDGPYAWG CF  E + Q  YC  S + WPC  G+ Y GRGPIQL+ N
Sbjct: 100 ISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLSWN 159

Query: 198 YNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMTPQGNKPSSHDVIIGKWQPSSAD 257
           YNYG AG ALG + L NP+ VA ++ ++FKTA+WFWMT Q  KPS H+V++ +++P+ AD
Sbjct: 160 YNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTKAD 219

Query: 258 NSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYKRYCDILNTSYGNNLDCYNQSPF 316
            +A R  GYG++TNIINGGLECG   D RV DR+G+++RY  +   + G NLDC NQ PF
Sbjct: 220 RAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQRPF 279

[Close]

BLAST of MS010658 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 201.8 bits (512), Expect = 8.0e-52
Identity = 123/316 (38.92%), Postives = 163/316 (51.58%), Query Frame = 0


Query: 4   KAHTLIILAFALFLGAASAEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQS-QCG 63
           KA  + +    +  G A ++ CG   NG  C   +CCSR+G+CG+T  YC  GCQS  C 
Sbjct: 10  KALIIFLFTLTIMTGTAFSQNCG--TNG--CKGNMCCSRWGYCGTTKAYCGTGCQSGPCN 69

Query: 64  DQ---TPTPSNPGS------GDVGSIITSAIFDQMLKYRNDARCPSNGFYSYNAFISAAR 123
            +   TPTPS  G       G + S+IT A F+ ++  +  + CP+ GFY+  AFI+AA 
Sbjct: 70  SKPKPTPTPSGSGGLNAGPRGTIASVITPAFFNSIMS-KVGSGCPAKGFYTRQAFIAAAE 129

Query: 124 SFPGFGTTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQDVYCTPS 183
           SF  +  T      KRE+AA L Q SHE             +  +C+  E  +  YC+PS
Sbjct: 130 SFAAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIARGRYCSPS 189

Query: 184 QQWPCAPGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKTAIWFWMT 243
             +PC PG+ YYGRGPIQ+T NYNYG AG  LGL LL +PD VA   TV+F+ A+WFW  
Sbjct: 190 TTYPCQPGKNYYGRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFW-- 249

Query: 244 PQGNKPSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVADRIGFYK 303
              NK           +P  +        G+G  T  INGG EC  G    V  R+  Y 
Sbjct: 250 ---NK---------NVRPVLSQ-------GFGATTRRINGG-ECNGGRPAAVQSRVNHYL 281

Query: 304 RYCDILNTSYGNNLDC 310
            +C  L  + G NL C
Sbjct: 310 DFCKKLGVTPGTNLSC 281

[Close]

BLAST of MS010658 vs. TAIR 10
Match: AT2G43620.1 (Chitinase family protein )

HSP 1 Score: 190.3 bits (482), Expect = 2.4e-48
Identity = 117/323 (36.22%), Postives = 160/323 (49.54%), Query Frame = 0


Query: 1   KKMKAHTLIILAFALFLGAAS--AEQCGRQANGALCPNRLCCSRFGWCGSTDEYCKDGCQ 60
           + M  +  I+  F L + A +  ++QCG       C   LCCSR+G+CG+TD YC  GC+
Sbjct: 5   RAMLKNAFILFLFTLTIMAKTVFSQQCGTTG----CAANLCCSRYGYCGTTDAYCGTGCR 64

Query: 61  S-QCGDQT----PTPSNPGSG-------DVGSIITSAIFDQMLKYRNDARCPSNGFYSYN 120
           S  C   T    PTPS    G        + +++T A FD ++    +  CP+ GFY+  
Sbjct: 65  SGPCSSSTTPIPPTPSGGAGGLNADPRDTIENVVTPAFFDGIMSKVGNG-CPAKGFYTRQ 124

Query: 121 AFISAARSFPGFGTTGDDATRKRELAAFLGQTSHETTGGWATAPDGPYAWGYCFIRERNQ 180
           AFI+AA+SF  +  T      KRE+AA L Q SHE             +  +C+  E  +
Sbjct: 125 AFIAAAQSFDAYKGT----VAKREIAAMLAQFSHE-------------SGSFCYKEEIAR 184

Query: 181 DVYCTPSQQWPCAPGQKYYGRGPIQLTHNYNYGPAGTALGLNLLNNPDSVATDATVSFKT 240
             YC+PS  +PC PG+ YYGRGPIQ+T NYNYG AG  LGL LL +PD VA    V+F+ 
Sbjct: 185 GKYCSPSTAYPCTPGKDYYGRGPIQITWNYNYGAAGKFLGLPLLTDPDMVARSPQVAFQC 244

Query: 241 AIWFWMTPQGNKPSSHDVIIGKWQPSSADNSAGRVPGYGVITNIINGGLECGHGPDDRVA 300
           A+WFW                     + +       G+G  T  INGG EC       V 
Sbjct: 245 AMWFW---------------------NLNVRPVLDQGFGATTRKINGG-ECNGRRPAAVQ 283

Query: 301 DRIGFYKRYCDILNTSYGNNLDC 310
            R+ +Y  +C  L  + G NL C
Sbjct: 305 SRVNYYLEFCRTLGITPGANLSC 283

The following BLAST results are available for this feature:

BLAST of MS010658 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132794.1	4.0e-186	97.12	endochitinase A-like [Momordica charantia]	[more]
ABD66068.1	8.3e-176	91.37	chitinase [Momordica charantia]	[more]
XP_022949791.1	5.9e-158	82.80	endochitinase-like [Cucurbita moschata]	[more]
XP_023544980.1	3.0e-157	82.48	endochitinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022977305.1	8.6e-157	82.80	chitinase 1-like [Cucurbita maxima]	[more]

BLAST of MS010658 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P36907	3.9e-136	70.00	Endochitinase OS=Pisum sativum OX=3888 PE=2 SV=1	[more]
P93680	9.6e-135	69.26	Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2	[more]
P08252	2.8e-134	69.94	Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2	[more]
Q9FRV1	3.6e-134	69.50	Basic endochitinase A OS=Secale cereale OX=4550 GN=rsca PE=1 SV=1	[more]
P24091	1.5e-132	71.01	Endochitinase B OS=Nicotiana tabacum OX=4097 GN=CHN50 PE=1 SV=1	[more]

BLAST of MS010658 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BU42	1.9e-186	97.12	endochitinase A-like OS=Momordica charantia OX=3673 GN=LOC111005566 PE=4 SV=1	[more]
Q207U1	4.0e-176	91.37	Chitinase OS=Momordica charantia OX=3673 GN=ChiA PE=4 SV=1	[more]
A0A6J1GD14	2.9e-158	82.80	endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453081 PE=4 SV=1	[more]
A0A6J1ILX6	4.2e-157	82.80	chitinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111477667 PE=4 SV=1	[more]
A0A6J1GD34	1.7e-155	81.01	endochitinase-like OS=Cucurbita moschata OX=3662 GN=LOC111453082 PE=4 SV=1	[more]

BLAST of MS010658 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12500.1	2.1e-132	68.45	basic chitinase	[more]
AT1G02360.1	6.3e-81	54.81	Chitinase family protein	[more]
AT4G01700.1	2.4e-80	55.00	Chitinase family protein	[more]
AT2G43610.1	8.0e-52	38.92	Chitinase family protein	[more]
AT2G43620.1	2.4e-48	36.22	Chitinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	PRINTS	PR00451	CHITINBINDNG	coord: 47..54 score: 57.17 coord: 32..40 score: 64.44 coord: 40..47 score: 75.83
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 24..61 e-value: 2.3E-16 score: 70.4
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 24..61 e-value: 1.7E-14 score: 54.0
IPR001002	Chitin-binding, type 1	PROSITE	PS50941	CHIT_BIND_I_2	coord: 22..63 score: 10.791357
None	No IPR available	GENE3D	3.30.20.10	Endochitinase, domain 2	coord: 162..218 e-value: 9.3E-120 score: 400.4
None	No IPR available	GENE3D	1.10.530.10		coord: 79..310 e-value: 9.3E-120 score: 400.4
None	No IPR available	PIRSR	PIRSR001060-2	PIRSR001060-2	coord: 2..315 e-value: 2.4E-145 score: 481.9
None	No IPR available	PANTHER	PTHR22595:SF171	CHITINASE (CLASS IB) / HEVEIN	coord: 5..315
None	No IPR available	PANTHER	PTHR22595	CHITINASE-RELATED	coord: 5..315
None	No IPR available	CDD	cd06921	ChtBD1_GH19_hevein	coord: 23..62 e-value: 1.59896E-19 score: 78.3
None	No IPR available	CDD	cd00325	chitinase_GH19	coord: 80..309 e-value: 5.73553E-115 score: 329.391
IPR016283	Glycoside hydrolase, family 19	PIRSF	PIRSF001060	Endochitinase	coord: 1..315 e-value: 2.9E-127 score: 422.1
IPR036861	Endochitinase-like superfamily	GENE3D	3.30.60.10		coord: 22..63 e-value: 5.5E-18 score: 66.8
IPR036861	Endochitinase-like superfamily	SUPERFAMILY	57016	Plant lectins/antimicrobial peptides	coord: 24..68
IPR000726	Glycoside hydrolase, family 19, catalytic	PFAM	PF00182	Glyco_hydro_19	coord: 79..309 e-value: 4.1E-131 score: 435.8
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00774	CHITINASE_19_2	coord: 222..232
IPR000726	Glycoside hydrolase, family 19, catalytic	PROSITE	PS00773	CHITINASE_19_1	coord: 97..119
IPR018371	Chitin-binding, type 1, conserved site	PROSITE	PS00026	CHIT_BIND_I_1	coord: 34..53
IPR023346	Lysozyme-like domain superfamily	SUPERFAMILY	53955	Lysozyme-like	coord: 75..315

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010658.1	MS010658.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0016998	cell wall macromolecule catabolic process
biological_process	GO:0006032	chitin catabolic process
molecular_function	GO:0004568	chitinase activity
molecular_function	GO:0008061	chitin binding