MS010627 (gene) Bitter gourd (TR) v1

Overview
NameMS010627
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionmethyltransferase-like protein 23
Locationscaffold35: 1081113 .. 1082793 (+)
RNA-Seq ExpressionMS010627
SyntenyMS010627
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGATTCCGTCTGCTCCGGCGAGCAATATGGAAGACGGTGACTCCGACGCGCCGGTCGGTGGCTCCTCAACAACCACATCTCGTCACGCTTTCGAAGACGACGCCGGAAAAACGGTCTTCTCTGTTTCAATAATCGAGGTGTCCAAAATCCACCTCTCATTCCTTTTTTTGTCTCCTTCAAAATTCCGGTAGACGGAGAAACTTTGAGTCCTTAACAATCTCTGTGTGTAATTTCGACGACTCTGCAGAATACGGAAGAGGAATACGGCCTTTATGTGTGGCCCTGTAGTATTGTTCTTGCCGAGTATGTTTGGCAGCAGAAAACTCGATTTGCTGGTGCAAATGTCGTTGAGGTACTTGTGTATTATCATTTTCTTTCCAGTGAAGTCTCCTGAAAACCTATAATCAGTAGATTTAGAGTGATATATTTGATTATTTTATAACGGGGAAGTTTAATGGCTAGGGTGACTCTGTGACCTCCGAATTGGTTGCAGCTCGGTGCTGGTACTTCATTGCCTGGATTGGTTGCGGCAAAATTGGGCGCTGATGTCACGTTGACTGATGATGCTAATAGAGTGGAGGTATTGCTTCGTCTTGGTGACTTCGCCTGAAAATATAATTTCCACTTAATGCACAAAAATTTCTATTAACCAATTTATATGAACTTCATGTTAGCTCTGTTTTTTGTTTGAGTTATGAATTATAAATACGTCAAAAAGCAAGGGAGTTTCTCGTATTACGAGGAGCCAGATTTTGTTTAGAAACCAAATTCATTTAGATAGTTATGTTAAGAGCGGACAGATAGATAGATGTTTGAGCGGCATATTTGCTTTCTAATGTCCGAATCTTCAATCTGCAGGTGCTGGAAAATATCAGGAGAGTTTGTGACCTGAATAATCTCAATTGCAATGTAAATCTATAAATTTTCTTGTTGTTGTAAGTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNATAAATTTTCTTGTTGTTGTAAGTTGTTTTTTTTTTTTTTTTTTTCTCTTTTTCTTCTGAAAGAATGGTTTTCTATCTGGCATAGTGACGAGTTTCTTATATGTGCAGGTTCTAGGACTGACATGGGGGGTCTGGGACATATCAATATTCAGTTTACGACCTACAATCATTATTGGGGCTGATGTTTTGTACGAAAACAGTGGTAGGACCGTAGGACCAATTTCTGATCTAATTTCATCTCTACTCTGCCATCTTTAATTTGTTTATGGAAAAAAAAATGTGCTGAAATTTGAGTTTGATTTGTTGTCGGGAAGCATTTGATGATCTCTTTTCTACCGTGGCCTTTCTTCTCCAAAGTTCACCAGGGTCAGTCTTTATAACAACCTACCACAATCGCAGGTAACTGATATTATTTCTTACATGATGGAAATTGACACAGCCACGGTGGTTATGTTATGATAGTAATTATTATTGCTGTCATTATTTTTCACCTTTACCAATTCACTGCCCTCCAAATTGTGTAGTGGGCATCATCTCATTGAGTTCTTGATGGTCAAATGGGGGTTGAAGTGTGAGAAGCTTCTTGATGGCTTTGCATTCATGCCATCGCATAAGGCATCAAACCTGAGTGGCAACATTCAATTGGCTGAGATTGTTCTTAATTGCGAACCAGTCAAGGTAATACAAAATGCATAT

mRNA sequence

CAGATTCCGTCTGCTCCGGCGAGCAATATGGAAGACGGTGACTCCGACGCGCCGGTCGGTGGCTCCTCAACAACCACATCTCGTCACGCTTTCGAAGACGACGCCGGAAAAACGGTCTTCTCTGTTTCAATAATCGAGAATACGGAAGAGGAATACGGCCTTTATGTGTGGCCCTGTAGTATTGTTCTTGCCGAGTATGTTTGGCAGCAGAAAACTCGATTTGCTGGTGCAAATGTCGTTGAGCTCGGTGCTGGTACTTCATTGCCTGGATTGGTTGCGGCAAAATTGGGCGCTGATGTCACGTTGACTGATGATGCTAATAGAGTGGAGGTGCTGGAAAATATCAGGAGAGTTTGTGACCTGAATAATCTCAATTGCAATGTTCTAGGACTGACATGGGGGGTCTGGGACATATCAATATTCAGTTTACGACCTACAATCATTATTGGGGCTGATGTTTTGTACGAAAACAGTGCATTTGATGATCTCTTTTCTACCGTGGCCTTTCTTCTCCAAAGTTCACCAGGGTCAGTCTTTATAACAACCTACCACAATCGCAGTGGGCATCATCTCATTGAGTTCTTGATGGTCAAATGGGGGTTGAAGTGTGAGAAGCTTCTTGATGGCTTTGCATTCATGCCATCGCATAAGGCATCAAACCTGAGTGGCAACATTCAATTGGCTGAGATTGTTCTTAATTGCGAACCAGTCAAGGTAATACAAAATGCATAT

Coding sequence (CDS)

CAGATTCCGTCTGCTCCGGCGAGCAATATGGAAGACGGTGACTCCGACGCGCCGGTCGGTGGCTCCTCAACAACCACATCTCGTCACGCTTTCGAAGACGACGCCGGAAAAACGGTCTTCTCTGTTTCAATAATCGAGAATACGGAAGAGGAATACGGCCTTTATGTGTGGCCCTGTAGTATTGTTCTTGCCGAGTATGTTTGGCAGCAGAAAACTCGATTTGCTGGTGCAAATGTCGTTGAGCTCGGTGCTGGTACTTCATTGCCTGGATTGGTTGCGGCAAAATTGGGCGCTGATGTCACGTTGACTGATGATGCTAATAGAGTGGAGGTGCTGGAAAATATCAGGAGAGTTTGTGACCTGAATAATCTCAATTGCAATGTTCTAGGACTGACATGGGGGGTCTGGGACATATCAATATTCAGTTTACGACCTACAATCATTATTGGGGCTGATGTTTTGTACGAAAACAGTGCATTTGATGATCTCTTTTCTACCGTGGCCTTTCTTCTCCAAAGTTCACCAGGGTCAGTCTTTATAACAACCTACCACAATCGCAGTGGGCATCATCTCATTGAGTTCTTGATGGTCAAATGGGGGTTGAAGTGTGAGAAGCTTCTTGATGGCTTTGCATTCATGCCATCGCATAAGGCATCAAACCTGAGTGGCAACATTCAATTGGCTGAGATTGTTCTTAATTGCGAACCAGTCAAGGTAATACAAAATGCATAT

Protein sequence

QIPSAPASNMEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVLGLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGHHLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY
Homology
BLAST of MS010627 vs. NCBI nr
Match: XP_022132584.1 (methyltransferase-like protein 23 isoform X2 [Momordica charantia])

HSP 1 Score: 473.8 bits (1218), Expect = 9.0e-130
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ
Sbjct: 1   MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL
Sbjct: 61  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY 245
           HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY
Sbjct: 181 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY 235

BLAST of MS010627 vs. NCBI nr
Match: XP_022132583.1 (methyltransferase-like protein 23 isoform X1 [Momordica charantia])

HSP 1 Score: 462.2 bits (1188), Expect = 2.7e-126
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ
Sbjct: 1   MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL
Sbjct: 61  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK
Sbjct: 181 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 229

BLAST of MS010627 vs. NCBI nr
Match: KAG6594758.1 (Methyltransferase-like protein 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421.0 bits (1081), Expect = 7.0e-114
Identity = 203/229 (88.65%), Postives = 218/229 (95.20%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDG SD     SSTT SRH FEDDAG++VF++SIIEN EE+YGLYVWPCSI+LAEY+WQ
Sbjct: 1   MEDGSSDDSAAPSSTTISRHVFEDDAGESVFTISIIENMEEDYGLYVWPCSIILAEYIWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRF+GANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIRRVCDLN LNCNV+
Sbjct: 61  QKTRFSGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRRVCDLNKLNCNVI 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWD SIF+LRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDTSIFNLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKL+DGFAFMPSHKA+NLSGNIQLAEIVLNC+PVK
Sbjct: 181 HLIEFLMVKWGLKCEKLVDGFAFMPSHKAANLSGNIQLAEIVLNCDPVK 229

BLAST of MS010627 vs. NCBI nr
Match: XP_022926494.1 (methyltransferase-like protein 23 [Cucurbita moschata])

HSP 1 Score: 420.6 bits (1080), Expect = 9.1e-114
Identity = 203/229 (88.65%), Postives = 217/229 (94.76%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDG SD     SSTT SRH FEDDAG++VF++SIIEN EE+YGLYVWPC I+LAEY+WQ
Sbjct: 1   MEDGSSDDSAAPSSTTISRHVFEDDAGESVFTISIIENMEEDYGLYVWPCGIILAEYIWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRF+GANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIRRVCDLN LNCNV+
Sbjct: 61  QKTRFSGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRRVCDLNKLNCNVI 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWD SIF+LRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDTSIFNLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKL+DGFAFMPSHKASNLSGNIQLAEIVLNC+PVK
Sbjct: 181 HLIEFLMVKWGLKCEKLVDGFAFMPSHKASNLSGNIQLAEIVLNCDPVK 229

BLAST of MS010627 vs. NCBI nr
Match: XP_023518702.1 (methyltransferase-like protein 23 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 420.2 bits (1079), Expect = 1.2e-113
Identity = 204/229 (89.08%), Postives = 216/229 (94.32%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDG  D     SSTT SRH FEDD GK+VFS+SIIEN EE+YGLYVWPCSI+LAEY+WQ
Sbjct: 1   MEDGSFDDSAAPSSTTISRHVFEDDDGKSVFSISIIENMEEDYGLYVWPCSIILAEYIWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRF+GANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIRRVCDLN LNCNV+
Sbjct: 61  QKTRFSGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRRVCDLNKLNCNVI 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWD SIF+LRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDTSIFNLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKL+DGFAFMPSHKASNLSGNIQLAEIVLNC+PVK
Sbjct: 181 HLIEFLMVKWGLKCEKLVDGFAFMPSHKASNLSGNIQLAEIVLNCDPVK 229

BLAST of MS010627 vs. ExPASy Swiss-Prot
Match: Q6DJF8 (Methyltransferase-like protein 23 OS=Xenopus laevis OX=8355 GN=mettl23 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.0e-35
Identity = 77/164 (46.95%), Postives = 104/164 (63.41%), Query Frame = 0

Query: 42  VSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVELGAGTSLPGLVAAKLGADVT 101
           V+I E    +YG+YVWPC++VLA+Y+W  +   A   V+E+GAG SLPG++AAK GA V 
Sbjct: 29  VTIPEVLNCQYGMYVWPCAVVLAQYLWYHRKNLADKRVLEVGAGVSLPGILAAKCGAKVI 88

Query: 102 LTDDANRVEVLENIRRVCDLNNL-NCNVLGLTWGVWDISIFSLRP-TIIIGADVLYENSA 161
           L+D A   + LEN RR C +NN+    V+GLTWG     +  L P  II+G+DV YE   
Sbjct: 89  LSDSAEMPQCLENCRRSCKMNNIVGVPVIGLTWGEVSPDLLDLPPIDIILGSDVFYEPKD 148

Query: 162 FDDLFSTVAFLLQSSPGSVFITTYHNRSGHHLIEFLMVKWGLKC 204
           F+D+  TV FL++  P + F TTY  RS    +E L+ KW LKC
Sbjct: 149 FEDILLTVRFLMERMPQAEFWTTYQVRSADWSVEALLCKWNLKC 192

BLAST of MS010627 vs. ExPASy Swiss-Prot
Match: A2AA28 (Methyltransferase-like protein 23 OS=Mus musculus OX=10090 GN=Mettl23 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 2.0e-31
Identity = 80/207 (38.65%), Postives = 115/207 (55.56%), Query Frame = 0

Query: 3   PSAPASNMEDGDSDAPVGGSSTTTSRHAFEDD----AGKTVFSVSIIENTEEEYGLYVWP 62
           P AP  +    DS     G    T    F ++      + V  V + +    +YG+YVWP
Sbjct: 9   PWAPPPDPASLDSPTCEPGLMAGTQLFRFREEPVPGGNRAVLEVRVPQVLHVQYGMYVWP 68

Query: 63  CSIVLAEYVWQQKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRV 122
           C++VLA+Y+W  +    G  V+E+GAG SLPG++AAK GA V L+D +     L+  R+ 
Sbjct: 69  CAVVLAQYLWFHRRSLPGKAVLEVGAGVSLPGILAAKCGAKVILSDSSEFPHCLDICRQS 128

Query: 123 CDLNNL-NCNVLGLTWGVWDISIFSLRP-TIIIGADVLYENSAFDDLFSTVAFLLQSSPG 182
           C +NNL    V+GLTWG     I SL P  II+G+DV +E   F+ + +TV FL+Q +P 
Sbjct: 129 CQMNNLPQVEVVGLTWGHISKDILSLPPQDIILGSDVFFEPEDFESILATVYFLMQKNPK 188

Query: 183 SVFITTYHNRSGHHLIEFLMVKWGLKC 204
             F +TY  RS    +E L+ KW +KC
Sbjct: 189 VQFWSTYQVRSADWSLEGLLYKWDMKC 215

BLAST of MS010627 vs. ExPASy Swiss-Prot
Match: Q5RJL2 (Methyltransferase-like protein 23 OS=Rattus norvegicus OX=10116 GN=Mettl23 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.6e-31
Identity = 73/179 (40.78%), Postives = 106/179 (59.22%), Query Frame = 0

Query: 31  FEDDAG----KTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVELGAGT 90
           F D+ G     TV  V + +    +YG+YVWPC++VLA+Y+W  +    G  V+E+GAG 
Sbjct: 9   FRDEPGPGADPTVLEVRVPQVLHVQYGMYVWPCAVVLAQYLWFHRRVLPGKAVLEIGAGV 68

Query: 91  SLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNL-NCNVLGLTWGVWDISIFSLRP 150
           SLPG++AAK GA VTL+D       L+   + C +NNL    ++GLTWG       SL P
Sbjct: 69  SLPGILAAKCGAKVTLSDSPELPHCLDICWQSCQMNNLPQVQIVGLTWGHISKDTLSLPP 128

Query: 151 -TIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGHHLIEFLMVKWGLKC 204
             II+G+DV +E   F+ + +TV FL+Q +P   F +TY  RS    +E L+ KW ++C
Sbjct: 129 QDIILGSDVFFEPEDFESILATVYFLMQKNPKVQFWSTYQVRSADWSLEGLLYKWDMRC 187

BLAST of MS010627 vs. ExPASy Swiss-Prot
Match: Q86XA0 (Methyltransferase-like protein 23 OS=Homo sapiens OX=9606 GN=METTL23 PE=1 SV=3)

HSP 1 Score: 129.8 bits (325), Expect = 4.2e-29
Identity = 63/152 (41.45%), Postives = 96/152 (63.16%), Query Frame = 0

Query: 54  LYVWPCSIVLAEYVWQQKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLE 113
           +YVWPC++VLA+Y+W  +    G  ++E+GAG SLPG++AAK GA+V L+D +     LE
Sbjct: 1   MYVWPCAVVLAQYLWFHRRSLPGKAILEIGAGVSLPGILAAKCGAEVILSDSSELPHCLE 60

Query: 114 NIRRVCDLNNL-NCNVLGLTWGVWDISIFSLRP-TIIIGADVLYENSAFDDLFSTVAFLL 173
             R+ C +NNL +  V+GLTWG     + +L P  II+ +DV +E   F+D+ +T+ FL+
Sbjct: 61  VCRQSCQMNNLPHLQVVGLTWGHISWDLLALPPQDIILASDVFFEPEDFEDILATIYFLM 120

Query: 174 QSSPGSVFITTYHNRSGHHLIEFLMVKWGLKC 204
             +P     +TY  RS    +E L+ KW +KC
Sbjct: 121 HKNPKVQLWSTYQVRSADWSLEALLYKWDMKC 152

BLAST of MS010627 vs. ExPASy Swiss-Prot
Match: A4FV42 (Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.5e-10
Identity = 47/126 (37.30%), Postives = 64/126 (50.79%), Query Frame = 0

Query: 56  VWPCSIVLAEYVWQQKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENI 115
           VW  ++VLA Y+        G + VELGAGT L G+VAA LGA VT+TD    +E L+  
Sbjct: 46  VWDAAVVLATYLEMGTVELRGCSAVELGAGTGLVGIVAALLGAHVTITDRKVALEFLK-- 105

Query: 116 RRVCDLNNLNCN----------VLGLTWGVWDISIFSLRP-TIIIGADVLYENSAFDDLF 171
                 +N+  N          V  LTWG  ++  FS     +I+GAD++Y    F DL 
Sbjct: 106 ------SNVQANLPPHIQPKAVVKELTWG-QNLGRFSPGEFDLILGADIIYLEETFTDLL 162

BLAST of MS010627 vs. ExPASy TrEMBL
Match: A0A6J1BTG2 (methyltransferase-like protein 23 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005410 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 4.4e-130
Identity = 235/235 (100.00%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ
Sbjct: 1   MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL
Sbjct: 61  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY 245
           HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY
Sbjct: 181 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQNAY 235

BLAST of MS010627 vs. ExPASy TrEMBL
Match: A0A6J1BWN5 (methyltransferase-like protein 23 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005410 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.3e-126
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ
Sbjct: 1   MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL
Sbjct: 61  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK
Sbjct: 181 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 229

BLAST of MS010627 vs. ExPASy TrEMBL
Match: A0A6J1EF96 (methyltransferase-like protein 23 OS=Cucurbita moschata OX=3662 GN=LOC111433627 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.4e-114
Identity = 203/229 (88.65%), Postives = 217/229 (94.76%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDG SD     SSTT SRH FEDDAG++VF++SIIEN EE+YGLYVWPC I+LAEY+WQ
Sbjct: 1   MEDGSSDDSAAPSSTTISRHVFEDDAGESVFTISIIENMEEDYGLYVWPCGIILAEYIWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRF+GANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIRRVCDLN LNCNV+
Sbjct: 61  QKTRFSGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRRVCDLNKLNCNVI 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWD SIF+LRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDTSIFNLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKL+DGFAFMPSHKASNLSGNIQLAEIVLNC+PVK
Sbjct: 181 HLIEFLMVKWGLKCEKLVDGFAFMPSHKASNLSGNIQLAEIVLNCDPVK 229

BLAST of MS010627 vs. ExPASy TrEMBL
Match: A0A6J1KNZ7 (methyltransferase-like protein 23 OS=Cucurbita maxima OX=3661 GN=LOC111497375 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.4e-113
Identity = 202/229 (88.21%), Postives = 216/229 (94.32%), Query Frame = 0

Query: 10  MEDGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQ 69
           MEDG SD     SSTT SRH FEDDAG++VF++SIIEN EE+YGLYVWPCSI+LAEY+WQ
Sbjct: 1   MEDGSSDDSAAPSSTTISRHVFEDDAGESVFTISIIENMEEDYGLYVWPCSIILAEYIWQ 60

Query: 70  QKTRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 129
           QKTRF+GANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIRRVCDLN LNCNV+
Sbjct: 61  QKTRFSGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRRVCDLNKLNCNVI 120

Query: 130 GLTWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 189
           GLTWGVWD SIF+LR TIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH
Sbjct: 121 GLTWGVWDTSIFNLRSTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGH 180

Query: 190 HLIEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVK 239
           HLIEFLMVKWGLKCEKL+DGFA MPSHKASNLSGNIQLAEIVLNC+PVK
Sbjct: 181 HLIEFLMVKWGLKCEKLVDGFALMPSHKASNLSGNIQLAEIVLNCDPVK 229

BLAST of MS010627 vs. ExPASy TrEMBL
Match: A0A1S3B244 (methyltransferase-like protein 23 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485159 PE=4 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 6.4e-113
Identity = 201/231 (87.01%), Postives = 218/231 (94.37%), Query Frame = 0

Query: 12  DGDSDAPVGGSSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQK 71
           DGDSD P   S TTTS H FEDDAGK+VFS++IIEN EEEYGLYVWPCSI+LAEYVWQQK
Sbjct: 10  DGDSDEPADPSLTTTSHHVFEDDAGKSVFSIAIIENMEEEYGLYVWPCSIILAEYVWQQK 69

Query: 72  TRFAGANVVELGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVLGL 131
            RFAGANVVELGAGTSLPGLVAAKLGA+VTLTDDANRVEVL+NIR+VCDLNNLNCN++GL
Sbjct: 70  ARFAGANVVELGAGTSLPGLVAAKLGANVTLTDDANRVEVLDNIRKVCDLNNLNCNIMGL 129

Query: 132 TWGVWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGHHL 191
           TWG+WDISIF+LRPTIIIGADVLYENSAFDDLFSTVAFLLQ+SPGSVFITTYHNRSGHHL
Sbjct: 130 TWGMWDISIFNLRPTIIIGADVLYENSAFDDLFSTVAFLLQNSPGSVFITTYHNRSGHHL 189

Query: 192 IEFLMVKWGLKCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCEPVKVIQN 243
           IEFLMVKWGLKCEKL+DGFAF+PS KAS LSGNIQLAEIVLNC  VKV+++
Sbjct: 190 IEFLMVKWGLKCEKLVDGFAFLPSQKASKLSGNIQLAEIVLNCGSVKVVES 240

BLAST of MS010627 vs. TAIR 10
Match: AT1G63855.3 (Putative methyltransferase family protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.0e-70
Identity = 132/214 (61.68%), Postives = 160/214 (74.77%), Query Frame = 0

Query: 22  SSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVE 81
           ++TT S+H F   AG +   +SIIEN +EEYGL+VWPCS++LAEYVWQ ++RF  ++++E
Sbjct: 5   TTTTISQHNFY-GAGDSETCISIIENLKEEYGLFVWPCSVILAEYVWQHRSRFRDSSILE 64

Query: 82  LGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVLGLTWGVWDISIF 141
           LGAGTSLPGLVAAK+GA+VTLTDDA + EVL+N+RRVC+LN LNCNVLGLTWGVWD  I 
Sbjct: 65  LGAGTSLPGLVAAKVGANVTLTDDATKPEVLDNMRRVCELNKLNCNVLGLTWGVWDAPIL 124

Query: 142 SLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSPGSVFITTYHNRSGHHLIEFLMVKWGL 201
            LRP II+GADVLY++S                            SGHHLIEFLMVKWGL
Sbjct: 125 DLRPNIILGADVLYDSSG---------------------------SGHHLIEFLMVKWGL 184

Query: 202 KCEKLLDGFAFMPSHKASNLSGNIQLAEIVLNCE 236
           KC KLLDGF+F+PS KAS LSGNIQL EIVL+ +
Sbjct: 185 KCVKLLDGFSFLPSQKASVLSGNIQLVEIVLSSQ 190

BLAST of MS010627 vs. TAIR 10
Match: AT1G63855.1 (Putative methyltransferase family protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.0e-50
Identity = 96/154 (62.34%), Postives = 120/154 (77.92%), Query Frame = 0

Query: 22  SSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVE 81
           ++TT S+H F   AG +   +SIIEN +EEYGL+VWPCS++LAEYVWQ ++RF  ++++E
Sbjct: 5   TTTTISQHNFY-GAGDSETCISIIENLKEEYGLFVWPCSVILAEYVWQHRSRFRDSSILE 64

Query: 82  LGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVLGLTWGVWDISIF 141
           LGAGTSLPGLVAAK+GA+VTLTDDA + EVL+N+RRVC+LN LNCNVLGLTWGVWD  I 
Sbjct: 65  LGAGTSLPGLVAAKVGANVTLTDDATKPEVLDNMRRVCELNKLNCNVLGLTWGVWDAPIL 124

Query: 142 SLRPTIIIGADVLYENSAFDDLFSTVAFLLQSSP 176
            LRP II+GADVLY++S    LF         SP
Sbjct: 125 DLRPNIILGADVLYDSSGSFHLFLFWVLAFSFSP 157

BLAST of MS010627 vs. TAIR 10
Match: AT1G63855.2 (Putative methyltransferase family protein )

HSP 1 Score: 148.3 bits (373), Expect = 8.1e-36
Identity = 70/108 (64.81%), Postives = 92/108 (85.19%), Query Frame = 0

Query: 22  SSTTTSRHAFEDDAGKTVFSVSIIENTEEEYGLYVWPCSIVLAEYVWQQKTRFAGANVVE 81
           ++TT S+H F   AG +   +SIIEN +EEYGL+VWPCS++LAEYVWQ ++RF  ++++E
Sbjct: 5   TTTTISQHNFY-GAGDSETCISIIENLKEEYGLFVWPCSVILAEYVWQHRSRFRDSSILE 64

Query: 82  LGAGTSLPGLVAAKLGADVTLTDDANRVEVLENIRRVCDLNNLNCNVL 130
           LGAGTSLPGLVAAK+GA+VTLTDDA + EVL+N+RRVC+LN LNCNV+
Sbjct: 65  LGAGTSLPGLVAAKVGANVTLTDDATKPEVLDNMRRVCELNKLNCNVI 111

BLAST of MS010627 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 55.5 bits (132), Expect = 7.2e-08
Identity = 41/119 (34.45%), Postives = 63/119 (52.94%), Query Frame = 0

Query: 78  NVVELGAGTSLPGL-VAAKLGADVTLTDDANRVEVLENIRRVCDLN-------NLNCNVL 137
           N+VELG+GT + G+  AA LGA+VT+TD  N   V+EN++   D N           +V 
Sbjct: 91  NIVELGSGTGIVGIAAAATLGANVTVTDLPN---VIENLKFNADANAQVVAKFGGKVHVA 150

Query: 138 GLTWG-VWDISIFSLRPTIIIGADVLYENSAFDDLFSTVAF-LLQSSPGSVFITTYHNR 187
            L WG + D+        +I+ +DV+Y    ++ L  T+ F LL+ S   VF+  +  R
Sbjct: 151 SLRWGEIDDVESLGQNVDLILASDVVYHVHLYEPLLKTLRFLLLEGSSERVFLMAHLKR 206

BLAST of MS010627 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 7.9e-07
Identity = 46/154 (29.87%), Postives = 74/154 (48.05%), Query Frame = 0

Query: 41  SVSIIE-----NTEEEYGLYVWPCSIVLAEY----VWQQKTRFAGANVVELGAGTSLPGL 100
           S+SI++     NT    G  +W   +VL ++    V  +     G  +VELG+G  L G 
Sbjct: 102 SLSILQSPCSLNTPGVTGSVMWDSGVVLGKFLEHSVDSKVLSLEGKKIVELGSGCGLVGC 161

Query: 101 VAAKLGADVTLTDDANRVEVL-ENIRRVCDLNNL--NCNVLGLTWG-VWDISIFSLRPTI 160
           +AA LG +  LTD  +R+ +L +NI+      N   +  V  L WG   D  +    P  
Sbjct: 162 IAALLGGNAVLTDLPDRLRLLKKNIQTNLHRGNTRGSAIVQELVWGDDPDPDLIEPFPDY 221

Query: 161 IIGADVLYENSAFDDLFSTVAFLLQSSPGSVFIT 182
           ++G+DV+Y   A   L  T+   L S   ++F++
Sbjct: 222 VLGSDVIYSEEAVHHLVKTL-LQLCSDQTTIFLS 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132584.19.0e-130100.00methyltransferase-like protein 23 isoform X2 [Momordica charantia][more]
XP_022132583.12.7e-126100.00methyltransferase-like protein 23 isoform X1 [Momordica charantia][more]
KAG6594758.17.0e-11488.65Methyltransferase-like protein 23, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022926494.19.1e-11488.65methyltransferase-like protein 23 [Cucurbita moschata][more]
XP_023518702.11.2e-11389.08methyltransferase-like protein 23 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6DJF81.0e-3546.95Methyltransferase-like protein 23 OS=Xenopus laevis OX=8355 GN=mettl23 PE=2 SV=1[more]
A2AA282.0e-3138.65Methyltransferase-like protein 23 OS=Mus musculus OX=10090 GN=Mettl23 PE=2 SV=1[more]
Q5RJL22.6e-3140.78Methyltransferase-like protein 23 OS=Rattus norvegicus OX=10116 GN=Mettl23 PE=2 ... [more]
Q86XA04.2e-2941.45Methyltransferase-like protein 23 OS=Homo sapiens OX=9606 GN=METTL23 PE=1 SV=3[more]
A4FV427.5e-1037.30Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE... [more]
Match NameE-valueIdentityDescription
A0A6J1BTG24.4e-130100.00methyltransferase-like protein 23 isoform X2 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1BWN51.3e-126100.00methyltransferase-like protein 23 isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1EF964.4e-11488.65methyltransferase-like protein 23 OS=Cucurbita moschata OX=3662 GN=LOC111433627 ... [more]
A0A6J1KNZ76.4e-11388.21methyltransferase-like protein 23 OS=Cucurbita maxima OX=3661 GN=LOC111497375 PE... [more]
A0A1S3B2446.4e-11387.01methyltransferase-like protein 23 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT1G63855.33.0e-7061.68Putative methyltransferase family protein [more]
AT1G63855.12.0e-5062.34Putative methyltransferase family protein [more]
AT1G63855.28.1e-3664.81Putative methyltransferase family protein [more]
AT3G50850.17.2e-0834.45Putative methyltransferase family protein [more]
AT1G73320.17.9e-0729.87S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 27..210
e-value: 2.8E-38
score: 133.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..30
NoneNo IPR availablePANTHERPTHR14614:SF2METHYLTRANSFERASE-LIKE PROTEIN 23coord: 24..228
IPR019410Lysine methyltransferasePFAMPF10294Methyltransf_16coord: 42..187
e-value: 1.0E-20
score: 74.2
IPR019410Lysine methyltransferasePANTHERPTHR14614HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGENcoord: 24..228
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 51..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010627.1MS010627.1mRNA