MS010624 (gene) Bitter gourd (TR) v1

BLAST of MS010624 vs. NCBI nr
Match: XP_022132660.1 (E3 ubiquitin-protein ligase RNF181 homolog [Momordica charantia])

HSP 1 Score: 273.1 bits (697), Expect = 1.2e-69
Identity = 128/136 (94.12%), Postives = 128/136 (94.12%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKD--------ESHVKQRAKKEGCEALAHPTLYDAVGRE 60
           MVYRKIISSFYNFVVLKLYPGIKD        ESHVKQRAKKEGCEALAHPTLYDAVGRE
Sbjct: 1   MVYRKIISSFYNFVVLKLYPGIKDALFCGDVIESHVKQRAKKEGCEALAHPTLYDAVGRE 60

Query: 61  WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL 120
           WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL
Sbjct: 61  WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL 120

Query: 121 TEEMVIWFTSFHVVGY 129
           TEEMVIWFTSFHVVGY
Sbjct: 121 TEEMVIWFTSFHVVGY 136

BLAST of MS010624 vs. NCBI nr
Match: XP_038883680.1 (E3 ubiquitin-protein ligase znrf3-like [Benincasa hispida])

HSP 1 Score: 170.2 bits (430), Expect = 1.1e-38
Identity = 84/136 (61.76%), Postives = 101/136 (74.26%), Query Frame = 0

Query: 2   VYRKIISSFYNFVVLKLYPGIKD--------ESHVKQRAKKEGCEALAHPTLYD-AVGRE 61
           + +KIISS +NF+ L+  PGIK+         S   QRAK++GCE   HPTL   A+  E
Sbjct: 40  ILQKIISSCFNFIALRSCPGIKNALLCVDSIASCFMQRAKEKGCETSTHPTLDGAAMETE 99

Query: 62  WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTTE 121
           WCSVCLSKVME EE+RV  LPC HE HKIC++RWFH+C  QKTCPIC+FW+RD+D QTTE
Sbjct: 100 WCSVCLSKVMEREEARV--LPCQHEVHKICVERWFHDCQKQKTCPICRFWMRDEDVQTTE 159

Query: 122 VLTEEMVIWFTSFHVV 127
           VLTEEMVIWFT F V+
Sbjct: 160 VLTEEMVIWFTCFQVI 173

BLAST of MS010624 vs. NCBI nr
Match: XP_022977421.1 (uncharacterized protein LOC111477769 isoform X1 [Cucurbita maxima])

HSP 1 Score: 160.6 bits (405), Expect = 8.9e-36
Identity = 76/122 (62.30%), Postives = 94/122 (77.05%), Query Frame = 0

Query: 12  NFVVLKLYPGIKD--ESHVKQRAKKEGCEALAHPTLYD-AVGREWCSVCLSKVMEGEESR 71
           +F V K++  + D  +S+  +RAK+EGCE  AHPTL D A+ REWCSVC S VME + ++
Sbjct: 50  SFPVSKMHYHVGDVIDSYFMKRAKEEGCETSAHPTLDDAAIEREWCSVCFSNVMERKVAQ 109

Query: 72  VLRLPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTTEVLTEEMVIWFTSFHVV 129
           +  LPC HEFHKIC++RWFHEC  QKTCPICQFW+RD+D Q TEVLTEEMVIW TS HV+
Sbjct: 110 I--LPCQHEFHKICVERWFHECQNQKTCPICQFWVRDEDCQPTEVLTEEMVIWSTSLHVI 169

BLAST of MS010624 vs. NCBI nr
Match: KAG6603894.1 (hypothetical protein SDJN03_04503, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 151.4 bits (381), Expect = 5.4e-33
Identity = 74/119 (62.18%), Postives = 88/119 (73.95%), Query Frame = 0

Query: 21  GIKD--------ESHVKQRAKKEGCEALAHPTLYD-AVGREWCSVCLSKVMEGEESRVLR 80
           GIKD        +S   +RAK+EGCE  AHPTL D A+ REWCSVCLS VME + +++  
Sbjct: 76  GIKDALSCGDVIDSCFMKRAKEEGCETSAHPTLDDAAMEREWCSVCLSNVMERKVAQI-- 135

Query: 81  LPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTTEVLTEEMVIWFTSFHVVGY 129
           LPC HEFHKIC++RWFHEC  QKTC IC+FW+ D+D Q TEVLTEEMVIW TS HV+ Y
Sbjct: 136 LPCQHEFHKICVERWFHECQNQKTCSICRFWVSDEDCQPTEVLTEEMVIWSTSLHVIDY 192

BLAST of MS010624 vs. NCBI nr
Match: XP_031276738.1 (E3 ubiquitin-protein ligase At1g12760 [Pistacia vera])

HSP 1 Score: 119.8 bits (299), Expect = 1.7e-23
Identity = 63/138 (45.65%), Postives = 86/138 (62.32%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKD-------ESHVKQRAK--KEGCEALAHPTLYDAVGR 60
           MV +K+ISS YN ++ +  P  K         S V+++A+       +LA  +L +    
Sbjct: 1   MVLKKLISSIYNLIIPRGVPRSKAAPSYEVIASGVREKAEDGASSSSSLAGASLAEDSEC 60

Query: 61  EWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDG-QTTE 120
           E C VCLS + EGE+ RV  LPCLHEFH+ CI RWF+ C KTCPIC+F +++D+     E
Sbjct: 61  ELCCVCLSGLKEGEDCRV--LPCLHEFHRACINRWFNACSKTCPICRFSMKEDENFHFRE 120

Query: 121 VLTEEMVIWFTSFHVVGY 129
             TEEMVIWF+SFHV G+
Sbjct: 121 EFTEEMVIWFSSFHVAGF 136

BLAST of MS010624 vs. ExPASy Swiss-Prot
Match: Q68DV7 (E3 ubiquitin-protein ligase RNF43 OS=Homo sapiens OX=9606 GN=RNF43 PE=1 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 3.7e-08
Identity = 23/52 (44.23%), Postives = 35/52 (67.31%), Query Frame = 0

Query: 54  CSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDD 106
           C++CL +  EG+E RV  + CLHEFH+ C+  W H+  +TCP+C F + + D
Sbjct: 272 CAICLEEFSEGQELRV--ISCLHEFHRNCVDPWLHQ-HRTCPLCMFNITEGD 320

BLAST of MS010624 vs. ExPASy Swiss-Prot
Match: Q6NRV8 (E3 ubiquitin-protein ligase arkadia-B OS=Xenopus laevis OX=8355 GN=rnf111-b PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.2e-08
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 26  SHVKQRAKKEGCEALAHPTLYDAVGREWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKR 85
           S  K  +K++G EA    T       E C++CLS + EGE+ R  RLPC+H FH++C+ +
Sbjct: 885 SQRKLHSKQDGEEATEEDT------EEKCTICLSILEEGEDVR--RLPCMHLFHQVCVDQ 944

Query: 86  WFHECQKTCPICQ 99
           W     K CPIC+
Sbjct: 945 WL-ITNKKCPICR 948

BLAST of MS010624 vs. ExPASy Swiss-Prot
Match: Q0V9R0 (E3 ubiquitin-protein ligase arkadia OS=Xenopus tropicalis OX=8364 GN=rnf111 PE=2 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 6.2e-08
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 26  SHVKQRAKKEGCEALAHPTLYDAVGREWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKR 85
           S  K  +K++G EA    T       E C++CLS + EGE+ R  RLPC+H FH++C+ +
Sbjct: 880 SQRKLHSKQDGEEATEEDT------EEKCTICLSILEEGEDVR--RLPCMHLFHQVCVDQ 939

Query: 86  WFHECQKTCPICQ 99
           W     K CPIC+
Sbjct: 940 WL-ITNKKCPICR 943

BLAST of MS010624 vs. ExPASy Swiss-Prot
Match: Q90ZT7 (E3 ubiquitin-protein ligase arkadia-A (Fragment) OS=Xenopus laevis OX=8355 GN=rnf111-a PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-07
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 26  SHVKQRAKKEGCEALAHPTLYDAVGREWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKR 85
           S  K  +K++G EA    T       E C++CLS + EGE+ R  RLPC+H FH++C+ +
Sbjct: 849 SQRKLHSKQDGEEAPEEDT------EEKCTICLSILEEGEDVR--RLPCMHLFHQVCVDQ 908

Query: 86  WFHECQKTCPICQ 99
           W     K CPIC+
Sbjct: 909 WL-ITNKKCPICR 912

BLAST of MS010624 vs. ExPASy Swiss-Prot
Match: Q66J97 (E3 ubiquitin-protein ligase arkadia-C OS=Xenopus laevis OX=8355 GN=rnf111-c PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-07
Identity = 30/73 (41.10%), Postives = 43/73 (58.90%), Query Frame = 0

Query: 26  SHVKQRAKKEGCEALAHPTLYDAVGREWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKR 85
           S  K  +K++G EA    T       E C++CLS + EGE+ R  RLPC+H FH++C+ +
Sbjct: 893 SQRKLHSKQDGEEAPEEDT------EEKCTICLSILEEGEDVR--RLPCMHLFHQVCVDQ 952

Query: 86  WFHECQKTCPICQ 99
           W     K CPIC+
Sbjct: 953 WL-ITNKKCPICR 956

BLAST of MS010624 vs. ExPASy TrEMBL
Match: A0A6J1BT35 (E3 ubiquitin-protein ligase RNF181 homolog OS=Momordica charantia OX=3673 GN=LOC111005465 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 6.0e-70
Identity = 128/136 (94.12%), Postives = 128/136 (94.12%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKD--------ESHVKQRAKKEGCEALAHPTLYDAVGRE 60
           MVYRKIISSFYNFVVLKLYPGIKD        ESHVKQRAKKEGCEALAHPTLYDAVGRE
Sbjct: 1   MVYRKIISSFYNFVVLKLYPGIKDALFCGDVIESHVKQRAKKEGCEALAHPTLYDAVGRE 60

Query: 61  WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL 120
           WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL
Sbjct: 61  WCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDGQTTEVL 120

Query: 121 TEEMVIWFTSFHVVGY 129
           TEEMVIWFTSFHVVGY
Sbjct: 121 TEEMVIWFTSFHVVGY 136

BLAST of MS010624 vs. ExPASy TrEMBL
Match: A0A6J1IJV3 (uncharacterized protein LOC111477769 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477769 PE=4 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 4.3e-36
Identity = 76/122 (62.30%), Postives = 94/122 (77.05%), Query Frame = 0

Query: 12  NFVVLKLYPGIKD--ESHVKQRAKKEGCEALAHPTLYD-AVGREWCSVCLSKVMEGEESR 71
           +F V K++  + D  +S+  +RAK+EGCE  AHPTL D A+ REWCSVC S VME + ++
Sbjct: 50  SFPVSKMHYHVGDVIDSYFMKRAKEEGCETSAHPTLDDAAIEREWCSVCFSNVMERKVAQ 109

Query: 72  VLRLPCLHEFHKICIKRWFHEC--QKTCPICQFWLRDDDGQTTEVLTEEMVIWFTSFHVV 129
           +  LPC HEFHKIC++RWFHEC  QKTCPICQFW+RD+D Q TEVLTEEMVIW TS HV+
Sbjct: 110 I--LPCQHEFHKICVERWFHECQNQKTCPICQFWVRDEDCQPTEVLTEEMVIWSTSLHVI 169

BLAST of MS010624 vs. ExPASy TrEMBL
Match: A0A5B6YSP6 (Putative E3 ubiquitin-protein ligase RHA2A (Fragment) OS=Davidia involucrata OX=16924 GN=Din_004126 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.5e-23
Identity = 61/137 (44.53%), Postives = 87/137 (63.50%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKD-------ESHVKQRAKKEGCEALAHPTLYDAVGREW 60
           MV +K++S   +F+ L+    IKD       ES+V  + K+   E        +A+  E 
Sbjct: 10  MVIKKLVSFICSFISLRGRRRIKDSSSYEVIESNVADKVKEGPDEPPGRLVRSEAIEVEL 69

Query: 61  CSVCLSKVME-GEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDG-QTTEV 120
           C VCLS++ E GE++RV  LPCLHEFH++C+ RWF+ C KTCP+C+F + D++  Q  E 
Sbjct: 70  CCVCLSRLKEGGEDTRV--LPCLHEFHRVCVDRWFNVCSKTCPVCRFLMEDEEKFQKRED 129

Query: 121 LTEEMVIWFTSFHVVGY 129
           LTEEMVIWF+SFH  G+
Sbjct: 130 LTEEMVIWFSSFHAAGF 144

BLAST of MS010624 vs. ExPASy TrEMBL
Match: A0A2I4FD42 (E3 ubiquitin-protein ligase RNF165-like OS=Juglans regia OX=51240 GN=LOC108997662 PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.8e-21
Identity = 56/140 (40.00%), Postives = 82/140 (58.57%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKDESHVKQRAKK-EGCEALAHPTLYDAVGR-------- 60
           M +RK++S  Y+F+  ++ P +K       R  K E  + +         G         
Sbjct: 1   MDFRKLVSFLYDFLGPRVQPSLKGFPSTSYRGLKWEDLQKVKEDPPMSPHGSGSRSVELL 60

Query: 61  --EWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDDG-QT 120
             E C VCLS++ +G+++R   LPCLHEFHK+C+ RWF  C+KTCP+C+F +  +D    
Sbjct: 61  EVEQCCVCLSRLKKGQDTRT--LPCLHEFHKVCVDRWFDACRKTCPVCRFSIGGEDNFHI 120

Query: 121 TEVLTEEMVIWFTSFHVVGY 129
            EVLT+EMVIWF+SFHV G+
Sbjct: 121 QEVLTDEMVIWFSSFHVAGF 138

BLAST of MS010624 vs. ExPASy TrEMBL
Match: A0A4S4DKH4 (RING-type domain-containing protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_030039 PE=4 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 2.3e-21
Identity = 60/135 (44.44%), Postives = 79/135 (58.52%), Query Frame = 0

Query: 1   MVYRKIISSFYNFVVLKLYPGIKDESHVKQRA------KKEGCEALAHPTLYDAVGREWC 60
           MV +K ISS    + LK    IK  S  +  A       KEG     H     A+  E C
Sbjct: 1   MVIKKFISSISTLIGLKGLQRIKHRSPCEAIAFNIGGVVKEGANDSRHG--QTALDGEMC 60

Query: 61  SVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDD-GQTTEVLT 120
            VCL ++ EGE+ RV  LPC HEFH+ C+ RW + CQKTCP+C+F + D++  +  E LT
Sbjct: 61  CVCLLRLEEGEDRRV--LPCRHEFHRACVNRWLNGCQKTCPVCRFLVEDEEKSRVKEALT 120

Query: 121 EEMVIWFTSFHVVGY 129
           +EMVIWF+SFHV G+
Sbjct: 121 DEMVIWFSSFHVAGF 131

BLAST of MS010624 vs. TAIR 10
Match: AT3G51325.1 (RING/U-box superfamily protein )

HSP 1 Score: 75.1 bits (183), Expect = 4.6e-14
Identity = 38/89 (42.70%), Postives = 56/89 (62.92%), Query Frame = 0

Query: 39  ALAHPTLYDAVGR--EWCSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQK-TCP 98
           AL +P +    GR  E CSVCL + ME ++  +  LPC HEFH +C+  WF+  +K  CP
Sbjct: 9   ALLYPRVQGGEGREEECCSVCLMR-MEAKDV-IKSLPCSHEFHSLCVDTWFNVSRKICCP 68

Query: 99  ICQFWLRDDDGQTTEVLTEEMVIWFTSFH 125
           +C+F        TT +LT+E+++WF+SFH
Sbjct: 69  LCRF------SPTTILLTDELLLWFSSFH 89

BLAST of MS010624 vs. TAIR 10
Match: AT1G15100.1 (RING-H2 finger A2A )

HSP 1 Score: 61.2 bits (147), Expect = 6.8e-10
Identity = 27/52 (51.92%), Postives = 34/52 (65.38%), Query Frame = 0

Query: 54  CSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQFWLRDDD 106
           C VCLSK+ EGEE R  +L C H FHK C++ W H+   TCP+C+  L  DD
Sbjct: 86  CVVCLSKLKEGEEVR--KLECRHVFHKKCLEGWLHQFNFTCPLCRSALVSDD 135

BLAST of MS010624 vs. TAIR 10
Match: AT3G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.1 bits (131), Expect = 4.9e-08
Identity = 27/57 (47.37%), Postives = 33/57 (57.89%), Query Frame = 0

Query: 54  CSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQF-----WLRDDD 106
           CSVCL +V  GE  R   LPCLH+FH  CI  W  + Q TCP+C+F     W   D+
Sbjct: 211 CSVCLEQVTVGEIVRT--LPCLHQFHAGCIDPWLRQ-QGTCPVCKFRAHSGWQEQDE 264

BLAST of MS010624 vs. TAIR 10
Match: AT5G45290.1 (RING/U-box superfamily protein )

HSP 1 Score: 52.0 bits (123), Expect = 4.2e-07
Identity = 19/45 (42.22%), Postives = 27/45 (60.00%), Query Frame = 0

Query: 54  CSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQ 99
           C +CL +  E +  R   LPC HEFHK C+ +W  E  + CP+C+
Sbjct: 490 CYICLVEYEEADSIRT--LPCHHEFHKTCVDKWLKEIHRVCPLCR 532

BLAST of MS010624 vs. TAIR 10
Match: AT2G01150.1 (RING-H2 finger protein 2B )

HSP 1 Score: 50.4 bits (119), Expect = 1.2e-06
Identity = 21/45 (46.67%), Postives = 27/45 (60.00%), Query Frame = 0

Query: 54  CSVCLSKVMEGEESRVLRLPCLHEFHKICIKRWFHECQKTCPICQ 99
           C VCLSK+  GEE R  +L C H FHK C++ W       CP+C+
Sbjct: 74  CIVCLSKLKTGEEVR--KLDCRHVFHKQCLEGWLQHLNFNCPLCR 116