MS010594 (gene) Bitter gourd (TR) v1

Overview


Name	MS010594
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	scaffold35: 848378 .. 852004 (+)
RNA-Seq Expression	MS010594
Synteny	MS010594

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGCCGATGACTCATCAGCATCTCCAACTCGAAGACTCTTTTCTTAGTAATTATAAGCCCTCCGAGCGCCGGATCGCGTCCGAATTTCTCACTACTTGGCTTCCCTTCCTCTCCAGAGATCTCTGCCCAGATTGCACCCAACTTCTCTCGGATCGAATCCGCGCCCTTGATCCAGGTATTTTTGTCTCTTCAGTAAGTTTCATCACGAGGTGTGTTAAAGAAAAGGGGCCATCTAATTTTAGATTTTTTTGAAATAATTGAGTGTAATCAAGGGAAATTTTAACGAGTCGGGGAAGCGATTTTTTTTGGGAGGGGTTCAGATTTTGAAATTTTTGCTATACTTTATGCATAGTTTTTTTAGCATGGAGCAGCGGGATAATTTTTTTAGTTCTACAAATGTGGGGCTGGTCGAACCTTCAACCATAGTGAAGATAAGGCACCCCTTATTCACTAAGCTATTCGTAGATTGGCGTTGGTGCATTAGGATAGAGCCCCCTTGATAAAGTTCCCCTTTCTCGGTTCTATTTTCTATTTCTTATTTCGTGTTTCTTCTTTCAGAAAAACAGGATTGCGATTGGTAATTATTGATTGTCTCTTGTTCTTTCTTTGTTTGTTGGCAAAAGAAACGAAATTGTGTCTTGTAACAATCTTTGGTTTCTTCTTCTCTCAATTATTAACTTAAAAAAAAATTAAATTTTTACATAAAAATTAATTTTTAAAAGAAAATCTGACGAAATTGTTGTTGATAATGCTATAGCTGTCCATTCTGATGAGAAATCAAGTTCTACACCCCCCATAGTGAAAGACATGCATGAGAGTAATGGGAATCAGGATGATCCTTGTGATACAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCGTTAGGGAGTTGGAAGGACAGCATAAATGAAGGGGCTGAAGCTGAGGGATTACCTGAAACTTCTTCAAGTGAACCACCCTCTAAGTTAAGTTCCACTAAGACCTCAGAGCCTCGAATGTCATGGGCTGACATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGAGTAGTCAATGTTAATGCGTCCACAGGGAAGTTAACAATATCAAAGATTAAGCCAAAGCTGTCCAGGGAGCAGAGAGAGTACATCAGGTTCATGAATGTCGGGCGGAAGAAAGATTTTATTTGTCTGGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACTGGTATTTTTAGTGCTGCGGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAGATGGGGAAGAAGGGAGAGTTAAGAGGTAATTATTCCCGTCTGCATATTGCTTTCTTTTATCTTCTGCTGAATGCCCCCCATTTTTCTATTTTTAATATTCTGAATTATTAGCTTACTTAACAGTTGACTTATTATGCTGCCATATGTTTATGATTCTTACAAGGGTAAGCTTAGTCTTTACTTCCTCAAGTCTCAGTAGTTAGTGCATTCAATGTGTAAATAAGGTTTAAGCCTCGAGGCTAAAAATTTGAACCTCACCTCAGGACACAAGCCTTATAGGCTTTTTAAAACGTTGTGCCTTGTGCCCTTGGATAATCTGGTTAATGACTCTACTATTGATGCTCATTTTCAATATGCCATGCTTTTGTAGTATCGATAAGCTGGTTCATGTATAAGATAGGGACTCATCCTATTCCTGATTAGTTTTTTGTTTAGTAAGTATGTCATCTACGATGTATTTGGGGTCATCCAATATTTTGTATAACTATTTCCAACAAACCTGTTTCTGTAGTATGTGATGATGTACCATGGGTCCTGATTAACTCTCCCGAAACTACCTACTCAATGATTGCTTACTTGATGCCTCACTACATTCCCTTCTGAACGTACATTCTCTCTCTTCTAAAACTGACATGATATTCCATTATTACCCTACATATGTATAAACTATAAAGGTGTGTAGTAGCCAGTAGGCTAGTATGTCTGGCTTTTAGATCTTTAAATCCATTCTGTAGATCCTGAGTTCACGCATCTTGGTTTTTATCCTTCTTAAGGGTGCTGGATAATTTTACTACTAATATAACAACTTTCTTGGGATACCATTCACAAACATAGGAGAAACAAAGAATTGTAAGGATAGCTAAACAGTAGCTGAGCTTCTATGTTTGTTAATTGGAATTTTAGAACAATTCTTCAACTAAGCCAATTTGAACCTGTAACCTTTTTTCTCTTCCTTTGTTGGTTGATTGGATATCAAATTCACAGACTTCATTGTTCATAGTGCATATTCCCTTGAAAATTTAATCTCTGTATGGAAGTGGAATCTCTTTTCAGATTTGATGAGAACAGAGGCTAATGTTTCTGCATCGTCCCCATTTTTGTTTTCAGATTCTTCGATTTTTTCCACTGCGCACAAACATGTCAATGACTTGAACACAATACCATACAACTCACCGTTTTAAATTTTTTTGCAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGACGTGTAACTCTTCAGTTTGGGTGCTGTTACAATTATGCACCTGTGAGTCTTTCAGCTGATTGCTAAGTTGGTTTGATTCTTTTGGATTGCATTTAATTTTTCATTTTTGATGTTCAACCAACTTTGATGCTCAGCTAGTATCACTAATTAGAGCATCATGTTTAATAAATGGCCAAATCCTTGCTAATAAAGTATTCTGACAGGACAAAAATGGTAATCCTCCTGGAGTTCTTCGAAATGAAATTGTGGATCCAATACCTAGTCTCTTTAAGGTGATCATCAGAAGGATGGTGAGATGGCACGTACTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTAAACATCTATGAAGAAGGGGACTGTATTCCTCCCCATATTGACAACCACGATTTCGTTCGACCTTTCTGCACTGTGTCATTCCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGTCCTGGTGAATTTTCTGGGCCGATTGCAATCCCACTGCCTGTAGGGTAAGTATAGTATGCAGTTGACTATGTTCATCTATTTCTGGTTCGATACCCATTTCCATCTTTCCAGGTTGGCAATCGCATTAGATTTCAAACCTTACTATTATGCATTTTCTTGCAGGTCTGTTCTCGTGTTAAATGGAAATGGAGCTGATGTCGCTAAGCATTGTGTACCCGCAGTCCCTACAAAGAGGTATAATTCAGGAGGGTCAATCAAACATCTTTACTTGCTTAATGTTATCCTTATCCAACCTAACTTGCCTCTTTTCTTGTCTCTTTTAGGATATCAATTACATTTAGAAGAATAGATGATTCCAAGCGGCCATTTGAGTATGCTTCCGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGTAAGTGAAAATGACGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTACGAGGGGTAGTCATATGAGAACCAGGGGATCTGGAAACCGAAGCGACACCCGTTTTGAGCCACGCAATTCAGGTAGGGCTCAACATAGGCCAGCAGATAATAGGAGGAGCAGAGTA

mRNA sequence

TTGCCGATGACTCATCAGCATCTCCAACTCGAAGACTCTTTTCTTAGTAATTATAAGCCCTCCGAGCGCCGGATCGCGTCCGAATTTCTCACTACTTGGCTTCCCTTCCTCTCCAGAGATCTCTGCCCAGATTGCACCCAACTTCTCTCGGATCGAATCCGCGCCCTTGATCCAGCTGTCCATTCTGATGAGAAATCAAGTTCTACACCCCCCATAGTGAAAGACATGCATGAGAGTAATGGGAATCAGGATGATCCTTGTGATACAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCGTTAGGGAGTTGGAAGGACAGCATAAATGAAGGGGCTGAAGCTGAGGGATTACCTGAAACTTCTTCAAGTGAACCACCCTCTAAGTTAAGTTCCACTAAGACCTCAGAGCCTCGAATGTCATGGGCTGACATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGAGTAGTCAATGTTAATGCGTCCACAGGGAAGTTAACAATATCAAAGATTAAGCCAAAGCTGTCCAGGGAGCAGAGAGAGTACATCAGGTTCATGAATGTCGGGCGGAAGAAAGATTTTATTTGTCTGGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACTGGTATTTTTAGTGCTGCGGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAGATGGGGAAGAAGGGAGAGTTAAGAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGACGTGTAACTCTTCAGTTTGGGTGCTGTTACAATTATGCACCTGACAAAAATGGTAATCCTCCTGGAGTTCTTCGAAATGAAATTGTGGATCCAATACCTAGTCTCTTTAAGGTGATCATCAGAAGGATGGTGAGATGGCACGTACTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTAAACATCTATGAAGAAGGGGACTGTATTCCTCCCCATATTGACAACCACGATTTCGTTCGACCTTTCTGCACTGTGTCATTCCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGTCCTGGTGAATTTTCTGGGCCGATTGCAATCCCACTGCCTGTAGGGTCTGTTCTCGTGTTAAATGGAAATGGAGCTGATGTCGCTAAGCATTGTGTACCCGCAGTCCCTACAAAGAGGATATCAATTACATTTAGAAGAATAGATGATTCCAAGCGGCCATTTGAGTATGCTTCCGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGTAAGTGAAAATGACGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTACGAGGGGTAGTCATATGAGAACCAGGGGATCTGGAAACCGAAGCGACACCCGTTTTGAGCCACGCAATTCAGGTAGGGCTCAACATAGGCCAGCAGATAATAGGAGGAGCAGAGTA

Coding sequence (CDS)

TTGCCGATGACTCATCAGCATCTCCAACTCGAAGACTCTTTTCTTAGTAATTATAAGCCCTCCGAGCGCCGGATCGCGTCCGAATTTCTCACTACTTGGCTTCCCTTCCTCTCCAGAGATCTCTGCCCAGATTGCACCCAACTTCTCTCGGATCGAATCCGCGCCCTTGATCCAGCTGTCCATTCTGATGAGAAATCAAGTTCTACACCCCCCATAGTGAAAGACATGCATGAGAGTAATGGGAATCAGGATGATCCTTGTGATACAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCGTTAGGGAGTTGGAAGGACAGCATAAATGAAGGGGCTGAAGCTGAGGGATTACCTGAAACTTCTTCAAGTGAACCACCCTCTAAGTTAAGTTCCACTAAGACCTCAGAGCCTCGAATGTCATGGGCTGACATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTGTGAATCAGAAAAAAGAGTAGTCAATGTTAATGCGTCCACAGGGAAGTTAACAATATCAAAGATTAAGCCAAAGCTGTCCAGGGAGCAGAGAGAGTACATCAGGTTCATGAATGTCGGGCGGAAGAAAGATTTTATTTGTCTGGAGAGGTTTAAAGGAAAATTAGTAAACATTCTTGAAGGACTTGAGCTTCATACTGGTATTTTTAGTGCTGCGGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAGATGGGGAAGAAGGGAGAGTTAAGAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGACGTGTAACTCTTCAGTTTGGGTGCTGTTACAATTATGCACCTGACAAAAATGGTAATCCTCCTGGAGTTCTTCGAAATGAAATTGTGGATCCAATACCTAGTCTCTTTAAGGTGATCATCAGAAGGATGGTGAGATGGCACGTACTTCCTCCAACATGTGTTCCTGATAGTTGCATTGTAAACATCTATGAAGAAGGGGACTGTATTCCTCCCCATATTGACAACCACGATTTCGTTCGACCTTTCTGCACTGTGTCATTCCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGTCCTGGTGAATTTTCTGGGCCGATTGCAATCCCACTGCCTGTAGGGTCTGTTCTCGTGTTAAATGGAAATGGAGCTGATGTCGCTAAGCATTGTGTACCCGCAGTCCCTACAAAGAGGATATCAATTACATTTAGAAGAATAGATGATTCCAAGCGGCCATTTGAGTATGCTTCCGAACCGGATTTGCAGGGAATCCAACCATTGCCCTATGAAGTAAGTGAAAATGACGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTACGAGGGGTAGTCATATGAGAACCAGGGGATCTGGAAACCGAAGCGACACCCGTTTTGAGCCACGCAATTCAGGTAGGGCTCAACATAGGCCAGCAGATAATAGGAGGAGCAGAGTA

Protein sequence

LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHSDEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKIKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTRFEPRNSGRAQHRPADNRRSRV

Homology

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BLAST of MS010594 vs. NCBI nr
Match: XP_022133128.1 (uncharacterized protein LOC111005802 [Momordica charantia])

HSP 1 Score: 1003.0 bits (2592), Expect = 8.8e-289
Identity = 499/501 (99.60%), Postives = 499/501 (99.60%), Query Frame = 0


Query: 3   MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS 62
           MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS
Sbjct: 1   MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS 60

Query: 63  DEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE 122
           DEKSSSTPPIVKDMH SNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE
Sbjct: 61  DEKSSSTPPIVKDMHGSNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE 120

Query: 123 TSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKIK 182
           TSSSEPPSKLSSTKTSEPRMSWADMTQEDELE EEDECESEKRVVNVNASTGKLTISKIK
Sbjct: 121 TSSSEPPSKLSSTKTSEPRMSWADMTQEDELEVEEDECESEKRVVNVNASTGKLTISKIK 180

Query: 183 PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA 242
           PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA
Sbjct: 181 PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA 240

Query: 243 LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS 302
           LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS
Sbjct: 241 LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS 300

Query: 303 LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV 362
           LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV
Sbjct: 301 LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV 360

Query: 363 FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK 422
           FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK
Sbjct: 361 FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK 420

Query: 423 RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR 482
           RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR
Sbjct: 421 RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR 480

Query: 483 FEPRNSGRAQHRPADNRRSRV 504
           FEPRNSGRAQHRPADNRRSRV
Sbjct: 481 FEPRNSGRAQHRPADNRRSRV 501

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BLAST of MS010594 vs. NCBI nr
Match: XP_038882153.1 (RNA demethylase ALKBH9B [Benincasa hispida])

HSP 1 Score: 883.6 bits (2282), Expect = 7.8e-253
Identity = 441/501 (88.02%), Postives = 464/501 (92.61%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCT+LLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTKLLSDRIRALDPAV 60

Query: 61  HSDEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGL 120
            S E   + PP V+DMHESNGNQDD CD NSLGSWKDE ETNSLGSWKD INEG EA+ +
Sbjct: 61  RSGENFGAAPP-VEDMHESNGNQDDACDANSLGSWKDEGETNSLGSWKDGINEGNEADVV 120

Query: 121 PETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISK 180
           PETSSSE  SKL+STKTS PRMSWADMTQEDELEEE+DE ESEKR+V++N ST KLTISK
Sbjct: 121 PETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEDDEYESEKRLVSINESTRKLTISK 180

Query: 181 I--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVD 240
           +  KP LSREQRE+IRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVD
Sbjct: 181 VIEKPTLSREQREHIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVD 240

Query: 241 HVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVD 300
           HVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPG+LR+EIVD
Sbjct: 241 HVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIVD 300

Query: 301 PIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSE 360
           PIPSLFKVIIRR+VRWHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSFLSE
Sbjct: 301 PIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLSE 360

Query: 361 CNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRI 420
           CNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRI
Sbjct: 361 CNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRI 420

Query: 421 DDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNR 480
           D+SKRP EYASEPDLQGIQPLPYE SENDVPTSPVSSEREIRRQPF RG HMR RGSGNR
Sbjct: 421 DESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGGHMRNRGSGNR 480

Query: 481 SDTRFEPRNSGRAQHRPADNR 500
           SDTRF+PRN GR +H  AD R
Sbjct: 481 SDTRFDPRNPGRGRHSSADRR 500

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BLAST of MS010594 vs. NCBI nr
Match: XP_023518278.1 (uncharacterized protein LOC111781804 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 874.0 bits (2257), Expect = 6.2e-250
Identity = 440/507 (86.79%), Postives = 470/507 (92.70%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  HSDEKSSSTPPI-VKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEG 120
           H DE SSS PP+  K M+ESNGN+DD CD NSLGSWKDEAETNSLGSW+D INEG EA+G
Sbjct: 61  HPDEDSSSAPPLREKAMNESNGNEDDACDANSLGSWKDEAETNSLGSWQDGINEGNEADG 120

Query: 121 LPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTIS 180
           LPETSSSEP SKL+STKTS PR+SWADMTQEDELEEEEDECE+EKR+VN NA  GKLTIS
Sbjct: 121 LPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNANA--GKLTIS 180

Query: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240
           K+  KPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKRIV 240

Query: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300
           DHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPG+L++EIV
Sbjct: 241 DHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSEIV 300

Query: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360
           D IPSLFKVIIRR+VRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSFLS
Sbjct: 301 DSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLS 360

Query: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420
           ECNIVFG+NLSIVGPG+FSGPIAIPLPVGSVLVLNGN ADVA+HCVPAVPTKRISITFRR
Sbjct: 361 ECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITFRR 420

Query: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTR-GSHMRTRGSG 480
           ID+SKRP EYA EPDLQGIQPLPYE S+NDVPTSPVSSEREIRRQPF R G HMRTRGSG
Sbjct: 421 IDESKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRGSG 480

Query: 481 NRSDTRFEPRNSGRAQHRPADNRRSRV 504
           NRSDT+FEPRN GRA++  +D RRSRV
Sbjct: 481 NRSDTQFEPRNPGRARYTSSD-RRSRV 504

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BLAST of MS010594 vs. NCBI nr
Match: XP_023544551.1 (uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544553.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868.6 bits (2243), Expect = 2.6e-248
Identity = 439/505 (86.93%), Postives = 469/505 (92.87%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC +CT+LLSDRIRALDPA 
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPAA 60

Query: 61  HSDEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGL 120
            +DE SSSTPP V+ MHESNGNQ+D CD NSLGSWKDEAETNSLGSWKD INEG EA+G+
Sbjct: 61  RTDENSSSTPP-VEVMHESNGNQNDACDANSLGSWKDEAETNSLGSWKDGINEGNEADGV 120

Query: 121 PETSSSEPPSKLSSTKTSEPRMSWADMTQEDEL-EEEEDECESEKRVVNVNASTGKLTIS 180
           PETSSS P SK+ STKTS PRMSWADMTQEDEL EEEEDE ESEKRVV+VN ST KLTIS
Sbjct: 121 PETSSSIPSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVVSVNESTRKLTIS 180

Query: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240
           K+  KPKLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240

Query: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300
           DH+Y+LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPG+LR+EIV
Sbjct: 241 DHIYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEIV 300

Query: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360
           DPIPSLFKVIIRR+VRWHVLPPTCVP+SCIVNIY+EGDCIPPHID+HDFVRPFCTVSFLS
Sbjct: 301 DPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHDFVRPFCTVSFLS 360

Query: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420
           ECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR
Sbjct: 361 ECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420

Query: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGN 480
           +D SK P EYA EPDLQGIQPLPYE SEN+VPTSPVSSEREIRRQPF RGSHMRTRGSGN
Sbjct: 421 MDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFRRGSHMRTRGSGN 480

Query: 481 RSDTRFEPRNSGRAQHRPADNRRSR 503
           R+DTRF+ RN GR +H  AD RRSR
Sbjct: 481 RTDTRFDSRNPGRGRHSSAD-RRSR 503

[Close]

BLAST of MS010594 vs. NCBI nr
Match: XP_023003874.1 (uncharacterized protein LOC111497336 [Cucurbita maxima])

HSP 1 Score: 863.2 bits (2229), Expect = 1.1e-246
Identity = 437/507 (86.19%), Postives = 466/507 (91.91%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  HSDEKSSS-TPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEG 120
             DE SSS +PP  KDM+ESNGN+ D CD NSLGSWKDEAETNSLGSW+D INEG EA+G
Sbjct: 61  RPDEDSSSASPPREKDMNESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEADG 120

Query: 121 LPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTIS 180
           LPETSSSEP SKL+STKTS PR+SWADMTQEDELEEEEDECE+EKR+VN NA  GKLTIS
Sbjct: 121 LPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRIVNANA--GKLTIS 180

Query: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240
           K+  KPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKRIV 240

Query: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300
           DHVYALQEMG KGEL+ERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPG+L++EIV
Sbjct: 241 DHVYALQEMGMKGELKERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSEIV 300

Query: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360
           D IPSLFKVIIRR+VRWHV+PPTCVPDSCIVNIY+E DCIPPHIDNHDFVRPFCTVSFLS
Sbjct: 301 DSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEEDCIPPHIDNHDFVRPFCTVSFLS 360

Query: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420
           ECNIVFG+NLSIVGPG+FSGPIAIPLPVGSVLVLNGN ADVA+HCVPAVPTKRISITFRR
Sbjct: 361 ECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITFRR 420

Query: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTR-GSHMRTRGSG 480
           ID+SKRP EYA EPDLQGIQPLPYE S+NDVPTS VSSEREIRRQPF R G HMRTRGSG
Sbjct: 421 IDESKRPIEYAPEPDLQGIQPLPYEASKNDVPTSHVSSEREIRRQPFRRGGGHMRTRGSG 480

Query: 481 NRSDTRFEPRNSGRAQHRPADNRRSRV 504
           NRSDT+FEPRN GRA H  +D RRSRV
Sbjct: 481 NRSDTQFEPRNPGRAWHTSSD-RRSRV 504

[Close]

BLAST of MS010594 vs. ExPASy Swiss-Prot
Match: Q9SL49 (RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.2e-155
Identity = 290/492 (58.94%), Postives = 360/492 (73.17%), Query Frame = 0


Query: 12  DSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHSD-------E 71
           D FL  Y+PSE +IASEFLT WLPFLS+DLC DC  LLS+RIR+LDPA  S+       E
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCSNNTDKVDGE 63

Query: 72  KSSSTPPIVKDM--HESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDS-INEGAEAEGLP 131
             + +  +V++M    ++ N DD  D  S     ++ + +SLGSWK S I  G+     P
Sbjct: 64  CKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGS----FP 123

Query: 132 ETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKI 191
           E  SS   S+ +  +T+ PRM WADM QEDE +EEE+E E E+           +   + 
Sbjct: 124 EDFSSVLQSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSMKTPE- 183

Query: 192 KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVY 251
           KPKLSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY
Sbjct: 184 KPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVY 243

Query: 252 ALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIP 311
            LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG+L+ E VDP+P
Sbjct: 244 QLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLP 303

Query: 312 SLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNI 371
            LFKVIIR++++WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+I
Sbjct: 304 HLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDI 363

Query: 372 VFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDS 431
           +FGSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++D+S
Sbjct: 364 LFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDES 423

Query: 432 KRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMR---TRGSGNR 489
           KRP  +  EPDLQGI+PLP +++ +        S     R     G++ R    RG GN 
Sbjct: 424 KRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNG 481

[Close]

BLAST of MS010594 vs. ExPASy Swiss-Prot
Match: Q9ZT92 (RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 3.0e-29
Identity = 80/248 (32.26%), Postives = 128/248 (51.61%), Query Frame = 0


Query: 202 KDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPK 261
           K F   E+ KG  VN+++GL+L+  +    E  +++D V  L+E G  G+L   +F    
Sbjct: 223 KGFQAKEQVKGHTVNVVKGLKLYEELLKEDEISKLLDFVAELREAGINGKLAGESFILFN 282

Query: 262 KWMKGKGRVTLQFGCCY--NYAPDKNGNPPGVLRNEIVDPIPSLFKVIIRRMVRWHVLPP 321
           K +KG  R  +Q G     +   D+N N      N  ++PIP L + +I   V W ++P 
Sbjct: 283 KQIKGNKRELIQLGVPIFGHVKADENSNDTNNSVN--IEPIPPLLESVIDHFVTWRLIPE 342

Query: 322 TCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPI 381
              P+ C++N +EEG+   P +      +P  T+  LSE  + +G  LS    G F GP+
Sbjct: 343 YKRPNGCVINFFEEGEYSQPFLKPPHLEQPISTL-VLSESTMAYGRILSSDNEGNFRGPL 402

Query: 382 AIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRI-DDSKRPFEYASEPDLQGIQP 441
            + L  GS+LV+ GN AD+A+H +     KR+SITF RI  D+       + P   G+  
Sbjct: 403 TLSLKQGSLLVMRGNSADMARHVMCPSQNKRVSITFFRIRPDTYHNHSQPNSPRNDGVMT 462

Query: 442 L--PYEVS 445
           +  PY+++
Sbjct: 463 MWQPYQMT 467

[Close]

BLAST of MS010594 vs. ExPASy Swiss-Prot
Match: Q66JG8 (RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 1.9e-12
Identity = 63/186 (33.87%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 227 IFSAAEQKRIVDHVYALQEMGKKGELRERTFS-AP--KKWMKGKGRVTLQFGCCYNYAPD 286
           +FS  E  RI   +  +    +KG  RE T   AP   K+  G+G         Y Y   
Sbjct: 60  LFSPDECARIEAKIDEVVSRAEKGLYREHTVDRAPLRNKYFFGEG---------YTYGAQ 119

Query: 287 KNGNPPGVLR---NEIVDPIPS-LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPP 346
                PG  R      VD IP+ + +++IRR+V   V+P   V +S ++N Y+ G CI  
Sbjct: 120 LQRRGPGQERLYPKGEVDEIPAWVNELVIRRLVEHRVIPEGFV-NSAVINDYQPGGCIVS 179

Query: 347 HIDN-HDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPI-AIPLPVGSVLVLNGNGAD 404
           H+D  H F RP  +VSF S+  + FG       P   S P+  +P+  GSV VL+G  AD
Sbjct: 180 HVDPIHIFERPIVSVSFFSDSALCFGCKFQF-KPIRVSEPVFFLPVQRGSVTVLSGYAAD 234

[Close]

BLAST of MS010594 vs. ExPASy TrEMBL
Match: A0A6J1BY69 (uncharacterized protein LOC111005802 OS=Momordica charantia OX=3673 GN=LOC111005802 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.3e-289
Identity = 499/501 (99.60%), Postives = 499/501 (99.60%), Query Frame = 0


Query: 3   MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS 62
           MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS
Sbjct: 1   MTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHS 60

Query: 63  DEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE 122
           DEKSSSTPPIVKDMH SNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE
Sbjct: 61  DEKSSSTPPIVKDMHGSNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPE 120

Query: 123 TSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKIK 182
           TSSSEPPSKLSSTKTSEPRMSWADMTQEDELE EEDECESEKRVVNVNASTGKLTISKIK
Sbjct: 121 TSSSEPPSKLSSTKTSEPRMSWADMTQEDELEVEEDECESEKRVVNVNASTGKLTISKIK 180

Query: 183 PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA 242
           PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA
Sbjct: 181 PKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYA 240

Query: 243 LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS 302
           LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS
Sbjct: 241 LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPS 300

Query: 303 LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV 362
           LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV
Sbjct: 301 LFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIV 360

Query: 363 FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK 422
           FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK
Sbjct: 361 FGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSK 420

Query: 423 RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR 482
           RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR
Sbjct: 421 RPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSGNRSDTR 480

Query: 483 FEPRNSGRAQHRPADNRRSRV 504
           FEPRNSGRAQHRPADNRRSRV
Sbjct: 481 FEPRNSGRAQHRPADNRRSRV 501

[Close]

BLAST of MS010594 vs. ExPASy TrEMBL
Match: A0A6J1KUK5 (uncharacterized protein LOC111497336 OS=Cucurbita maxima OX=3661 GN=LOC111497336 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.3e-247
Identity = 437/507 (86.19%), Postives = 466/507 (91.91%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  HSDEKSSS-TPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEG 120
             DE SSS +PP  KDM+ESNGN+ D CD NSLGSWKDEAETNSLGSW+D INEG EA+G
Sbjct: 61  RPDEDSSSASPPREKDMNESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEADG 120

Query: 121 LPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTIS 180
           LPETSSSEP SKL+STKTS PR+SWADMTQEDELEEEEDECE+EKR+VN NA  GKLTIS
Sbjct: 121 LPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRIVNANA--GKLTIS 180

Query: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240
           K+  KPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKRIV 240

Query: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300
           DHVYALQEMG KGEL+ERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPG+L++EIV
Sbjct: 241 DHVYALQEMGMKGELKERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSEIV 300

Query: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360
           D IPSLFKVIIRR+VRWHV+PPTCVPDSCIVNIY+E DCIPPHIDNHDFVRPFCTVSFLS
Sbjct: 301 DSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEEDCIPPHIDNHDFVRPFCTVSFLS 360

Query: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420
           ECNIVFG+NLSIVGPG+FSGPIAIPLPVGSVLVLNGN ADVA+HCVPAVPTKRISITFRR
Sbjct: 361 ECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITFRR 420

Query: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTR-GSHMRTRGSG 480
           ID+SKRP EYA EPDLQGIQPLPYE S+NDVPTS VSSEREIRRQPF R G HMRTRGSG
Sbjct: 421 IDESKRPIEYAPEPDLQGIQPLPYEASKNDVPTSHVSSEREIRRQPFRRGGGHMRTRGSG 480

Query: 481 NRSDTRFEPRNSGRAQHRPADNRRSRV 504
           NRSDT+FEPRN GRA H  +D RRSRV
Sbjct: 481 NRSDTQFEPRNPGRAWHTSSD-RRSRV 504

[Close]

BLAST of MS010594 vs. ExPASy TrEMBL
Match: A0A6J1EJB3 (uncharacterized protein LOC111433825 OS=Cucurbita moschata OX=3662 GN=LOC111433825 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 5.3e-247
Identity = 437/507 (86.19%), Postives = 465/507 (91.72%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC DCTQLLSDRIRALDPAV
Sbjct: 1   MAISHQHLKLEDPFLQNYKPSELRIASEFLTTWLPFLSRDLCQDCTQLLSDRIRALDPAV 60

Query: 61  HSDEKSSST-PPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEG 120
             +E SSS  PP  KDM ESNGN+ D CD NSLGSWKDEAETNSLGSW+D INEG EA+G
Sbjct: 61  RPNEDSSSAPPPREKDMSESNGNEVDACDANSLGSWKDEAETNSLGSWQDGINEGNEADG 120

Query: 121 LPETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTIS 180
           LPETSSSEP SKL+STKTS PR+SWADMTQEDELEEEEDECE+EKR+VN NA  GKLTIS
Sbjct: 121 LPETSSSEPSSKLNSTKTSGPRLSWADMTQEDELEEEEDECETEKRLVNANA--GKLTIS 180

Query: 181 KI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIV 240
           K+  KPKLSREQREYIRFM+VGRKKDFICLERFKGK VNILEGLELHTGIFSAAEQKRIV
Sbjct: 181 KVIEKPKLSREQREYIRFMSVGRKKDFICLERFKGKFVNILEGLELHTGIFSAAEQKRIV 240

Query: 241 DHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIV 300
           DHVYALQEMG KGELRERTFSAPKKWMKGKGRVTLQFGCCYNYA DKNGNPPG+L++EIV
Sbjct: 241 DHVYALQEMGMKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAADKNGNPPGILQSEIV 300

Query: 301 DPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLS 360
           D IPSLFKVIIRR+VRWHV+PPTCVPDSCIVNIY+EGDCIPPHIDNHDFVRPFCTVSFLS
Sbjct: 301 DSIPSLFKVIIRRLVRWHVIPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLS 360

Query: 361 ECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRR 420
           ECNIVFG+NLSIVGPG+FSGPIAIPLPVGSVLVLNGN ADVA+HCVPAVPTKRISITFRR
Sbjct: 361 ECNIVFGTNLSIVGPGQFSGPIAIPLPVGSVLVLNGNSADVARHCVPAVPTKRISITFRR 420

Query: 421 IDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTR-GSHMRTRGSG 480
           ID+ KRP EYA EPDLQGIQPLPYE S+NDVPTSPVSSEREIRRQPF R G HMRTRGSG
Sbjct: 421 IDEPKRPIEYAPEPDLQGIQPLPYEASKNDVPTSPVSSEREIRRQPFRRGGGHMRTRGSG 480

Query: 481 NRSDTRFEPRNSGRAQHRPADNRRSRV 504
           NRS+T FEPRN GRA+H  +D RRSRV
Sbjct: 481 NRSNTLFEPRNPGRARHTSSD-RRSRV 504

[Close]

BLAST of MS010594 vs. ExPASy TrEMBL
Match: A0A6J1IMQ3 (RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1)

HSP 1 Score: 861.7 bits (2225), Expect = 1.5e-246
Identity = 437/506 (86.36%), Postives = 467/506 (92.29%), Query Frame = 0


Query: 1   LPMTHQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAV 60
           + ++HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC +CT+LLSDRIRALDPA 
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPAS 60

Query: 61  HSDEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGL 120
            +DE SSSTPP V+ MHESNGNQ+D CD NSLGSWKDEAETNSLGSWKD INEG EA+G+
Sbjct: 61  RTDENSSSTPP-VEVMHESNGNQNDACDANSLGSWKDEAETNSLGSWKDGINEGNEADGV 120

Query: 121 PETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEE--DECESEKRVVNVNASTGKLTI 180
           PETSSS P +K+ S KTS PRMSWADMTQEDELEEEE  DE ESEKRVV+VN ST KLTI
Sbjct: 121 PETSSSVPSTKVVSIKTSGPRMSWADMTQEDELEEEEEDDEYESEKRVVSVNESTRKLTI 180

Query: 181 SKI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRI 240
           SK+  KPKLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRI
Sbjct: 181 SKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRI 240

Query: 241 VDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEI 300
           VDHVY LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPG+LR+EI
Sbjct: 241 VDHVYLLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGILRSEI 300

Query: 301 VDPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFL 360
           VDPIPSLFKVIIRR+VRWHVLPPTCVP+SCIVNIY+EGDCIPPHID+HDFVRPFCTVSFL
Sbjct: 301 VDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHDFVRPFCTVSFL 360

Query: 361 SECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR 420
           SECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR
Sbjct: 361 SECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR 420

Query: 421 RIDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGSG 480
           R+D+SK P EYA EPDLQGIQPLPYE SEN+VPTSPVSSEREIRRQ F RGSHMRTRGSG
Sbjct: 421 RMDESKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQSFRRGSHMRTRGSG 480

Query: 481 NRSDTRFEPRNSGRAQHRPADNRRSR 503
           NR+DTRF+ RN GR +H  AD RRSR
Sbjct: 481 NRTDTRFDSRNLGRGRHSSAD-RRSR 504

[Close]

BLAST of MS010594 vs. ExPASy TrEMBL
Match: A0A6J1GGD7 (uncharacterized protein LOC111453671 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453671 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 2.6e-246
Identity = 440/507 (86.79%), Postives = 467/507 (92.11%), Query Frame = 0


Query: 1   LPM--THQHLQLEDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDP 60
           LPM  +HQHL+LED FL NYKPSE RIASEFLTTWLPFLSRDLC +CT+LLSDRIRALDP
Sbjct: 38  LPMAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP 97

Query: 61  AVHSDEKSSSTPPIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAE 120
           A  +DE SSSTPP V+ MHESNGNQ+D CD NSLGSWKDEAETNSL SWKD INEG EA+
Sbjct: 98  AARTDENSSSTPP-VEVMHESNGNQNDACDANSLGSWKDEAETNSLESWKDGINEGNEAD 157

Query: 121 GLPETSSSEPPSKLSSTKTSEPRMSWADMTQEDEL-EEEEDECESEKRVVNVNASTGKLT 180
           G+PETSSS   SK+ STKTS PRMSWADMTQEDEL EEEEDE ESEKRVV+VN ST KLT
Sbjct: 158 GVPETSSSVQSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVVSVNESTRKLT 217

Query: 181 ISKI--KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 240
           ISK+  K KLSREQRE+IRF+NVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR
Sbjct: 218 ISKVIEKAKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKR 277

Query: 241 IVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNE 300
           IVDHVY+LQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPG++R+E
Sbjct: 278 IVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGIIRSE 337

Query: 301 IVDPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSF 360
           IVDPIPSLFKVIIRR+VRWHVLPPTCVP+SCIVNIY EGDCIPPHID+HDFVRPFCTVSF
Sbjct: 338 IVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYNEGDCIPPHIDSHDFVRPFCTVSF 397

Query: 361 LSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITF 420
           LSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITF
Sbjct: 398 LSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITF 457

Query: 421 RRIDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMRTRGS 480
           RR+D SK P EYA EPDLQGIQPLPYE SEN+VPTSPVSSEREIRRQPF RGSHMRTRGS
Sbjct: 458 RRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFRRGSHMRTRGS 517

Query: 481 GNRSDTRFEPRNSGRAQHRPADNRRSR 503
           GNR+DTRF+ RNSGR +H  AD RRSR
Sbjct: 518 GNRTDTRFDSRNSGRGRHSSAD-RRSR 542

[Close]

BLAST of MS010594 vs. TAIR 10
Match: AT2G17970.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 551.2 bits (1419), Expect = 8.6e-157
Identity = 290/492 (58.94%), Postives = 360/492 (73.17%), Query Frame = 0


Query: 12  DSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHSD-------E 71
           D FL  Y+PSE +IASEFLT WLPFLS+DLC DC  LLS+RIR+LDPA  S+       E
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCSNNTDKVDGE 63

Query: 72  KSSSTPPIVKDM--HESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDS-INEGAEAEGLP 131
             + +  +V++M    ++ N DD  D  S     ++ + +SLGSWK S I  G+     P
Sbjct: 64  CKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWKGSEIVFGS----FP 123

Query: 132 ETSSSEPPSKLSSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKI 191
           E  SS   S+ +  +T+ PRM WADM QEDE +EEE+E E E+           +   + 
Sbjct: 124 EDFSSVLQSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSMKTPE- 183

Query: 192 KPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVY 251
           KPKLSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY
Sbjct: 184 KPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVY 243

Query: 252 ALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIP 311
            LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG+L+ E VDP+P
Sbjct: 244 QLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLP 303

Query: 312 SLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNI 371
            LFKVIIR++++WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+I
Sbjct: 304 HLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDI 363

Query: 372 VFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDS 431
           +FGSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++D+S
Sbjct: 364 LFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDES 423

Query: 432 KRPFEYASEPDLQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMR---TRGSGNR 489
           KRP  +  EPDLQGI+PLP +++ +        S     R     G++ R    RG GN 
Sbjct: 424 KRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNG 481

[Close]

BLAST of MS010594 vs. TAIR 10
Match: AT4G36090.3 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 515.8 bits (1327), Expect = 4.0e-146
Identity = 288/511 (56.36%), Postives = 357/511 (69.86%), Query Frame = 0


Query: 11  EDSFLSNYKPSERRIASEFLTTWLPFLSRDLCPDCTQLLSDRIRALDPAVHSDEKSSSTP 70
           ED FL+ Y+ SE +IASEFLT WLPFLSRDLC DC  +LSDRIR+LDP   S+ +  +  
Sbjct: 7   EDVFLAKYQSSELKIASEFLTNWLPFLSRDLCNDCAHVLSDRIRSLDPEHCSNGEVKAGS 66

Query: 71  PIVKDMHESNGNQDDPCDTNSLGSWKDEAETNSL--GSWKDSINEGAEAEGLPETSSSE- 130
             + D  ES   + D  ++  + +  D      L      DS+  GA   G    S S  
Sbjct: 67  GSMVDNMES--EEVDNMESEEVDNKMDNIYDGKLCVSHSLDSLKAGAFVFGSLSDSVSHA 126

Query: 131 -PPSKLSSTKTSEPRMSWADMTQEDELEEEED-ECESEKRVVNVNASTGKLTISKIKPKL 190
            P S+ + ++ +  +MSWADM +ED LEEE+  E E     V+V+ S G    +  K KL
Sbjct: 127 MPSSQSTVSEAASAQMSWADMGEEDGLEEEDQKENELGSHGVDVSPSVGDSMKTPEKRKL 186

Query: 191 SREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQE 250
           SRE+RE  RFMNV + K F C E+ +G+ VNILEGLELHTG+FSA EQK+IVD VY LQE
Sbjct: 187 SREERERYRFMNVKKMKVFSCYEKVRGRSVNILEGLELHTGVFSAVEQKKIVDFVYELQE 246

Query: 251 MGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPSLFK 310
            G++GELRERTF+AP KWM+GKGRVT+QFGCCYNYAPDK GNPPG+L+   VDP+PS+FK
Sbjct: 247 KGRRGELRERTFTAPHKWMRGKGRVTIQFGCCYNYAPDKAGNPPGILQRGDVDPMPSIFK 306

Query: 311 VIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGS 370
           VII+R+V WHVLPPTCVPDSCIVNIYEE DCIPPHIDNHDF+RPFCTVSFLSECNI+FGS
Sbjct: 307 VIIKRLVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRPFCTVSFLSECNILFGS 366

Query: 371 NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSKRPF 430
           NL ++GPGEFSG  +IPLPVGSVLVL GNGADVAKHCVPAVPTKRISITFR++D+SKRP 
Sbjct: 367 NLKVLGPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTKRISITFRKMDESKRPV 426

Query: 431 EYASEPDLQGIQPLPYEVSENDVPTSPV-SSEREIRRQPFTRGSHMRTRGSGNR------ 490
            +  EPDL+ I+PLPYE +    P   V SS R    Q  +  ++    G G++      
Sbjct: 427 GFTPEPDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSNHNNRTAHGGGSKYRRSRD 486

Query: 491 --SDTRFEPRNSGR----AQHRPADNRRSRV 504
             S++R E  +SG+    ++H P  + R +V
Sbjct: 487 YHSESR-ERSSSGQRRETSRHTPNRSYRPKV 514

[Close]

BLAST of MS010594 vs. TAIR 10
Match: AT2G17970.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.6e-137
Identity = 249/421 (59.14%), Postives = 306/421 (72.68%), Query Frame = 0


Query: 73  VKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPETSSSEPPSKL 132
           V D ++      + CD +SLGSWK        GS+             PE  SS   S+ 
Sbjct: 10  VDDNYDEKSENGEDCDNHSLGSWKGSEIV--FGSF-------------PEDFSSVLQSRP 69

Query: 133 SSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKIKPKLSREQREY 192
           +  +T+ PRM WADM QEDE +EEE+E E E+           +   + KPKLSR+QRE 
Sbjct: 70  AVVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSMKTPE-KPKLSRDQREN 129

Query: 193 IRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGEL 252
           +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY LQE G++GEL
Sbjct: 130 LRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQLQEKGRRGEL 189

Query: 253 RERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPSLFKVIIRRMV 312
           ++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG+L+ E VDP+P LFKVIIR+++
Sbjct: 190 KKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHLFKVIIRKLI 249

Query: 313 RWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGP 372
           +WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+I+FGSNL + GP
Sbjct: 250 KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVEGP 309

Query: 373 GEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSKRPFEYASEPD 432
           G+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++D+SKRP  +  EPD
Sbjct: 310 GDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPEPD 369

Query: 433 LQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMR---TRGSGNRSDTR--FEPRN 489
           LQGI+PLP +++ +        S     R     G++ R    RG GN  D+R  + P  
Sbjct: 370 LQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYDSRGYYNPER 407

[Close]

BLAST of MS010594 vs. TAIR 10
Match: AT2G17970.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.6e-137
Identity = 249/421 (59.14%), Postives = 306/421 (72.68%), Query Frame = 0


Query: 73  VKDMHESNGNQDDPCDTNSLGSWKDEAETNSLGSWKDSINEGAEAEGLPETSSSEPPSKL 132
           V D ++      + CD +SLGSWK        GS+             PE  SS   S+ 
Sbjct: 10  VDDNYDEKSENGEDCDNHSLGSWKGSEIV--FGSF-------------PEDFSSVLQSRP 69

Query: 133 SSTKTSEPRMSWADMTQEDELEEEEDECESEKRVVNVNASTGKLTISKIKPKLSREQREY 192
           +  +T+ PRM WADM QEDE +EEE+E E E+           +   + KPKLSR+QRE 
Sbjct: 70  AVVETASPRMRWADMAQEDEFDEEEEEEEEERDSSRKGFDASSMKTPE-KPKLSRDQREN 129

Query: 193 IRFMNVGRKKDFICLERFKGKLVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGEL 252
           +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY LQE G++GEL
Sbjct: 130 LRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQLQEKGRRGEL 189

Query: 253 RERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNGNPPGVLRNEIVDPIPSLFKVIIRRMV 312
           ++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG+L+ E VDP+P LFKVIIR+++
Sbjct: 190 KKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHLFKVIIRKLI 249

Query: 313 RWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGP 372
           +WHVLPPTCVPDSCIVNIY+EGDCIPPHIDNHDF+RPFCT+SFLSEC+I+FGSNL + GP
Sbjct: 250 KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILFGSNLKVEGP 309

Query: 373 GEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDDSKRPFEYASEPD 432
           G+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++D+SKRP  +  EPD
Sbjct: 310 GDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKRPVWFTPEPD 369

Query: 433 LQGIQPLPYEVSENDVPTSPVSSEREIRRQPFTRGSHMR---TRGSGNRSDTR--FEPRN 489
           LQGI+PLP +++ +        S     R     G++ R    RG GN  D+R  + P  
Sbjct: 370 LQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYDSRGYYNPER 407

[Close]

BLAST of MS010594 vs. TAIR 10
Match: AT4G36090.2 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 457.2 bits (1175), Expect = 1.7e-128
Identity = 246/411 (59.85%), Postives = 301/411 (73.24%), Query Frame = 0


Query: 109 DSINEGAEAEGLPETSSSE--PPSKLSSTKTSEPRMSWADMTQEDELEEEED-ECESEKR 168
           DS+  GA   G    S S   P S+ + ++ +  +MSWADM +ED LEEE+  E E    
Sbjct: 37  DSLKAGAFVFGSLSDSVSHAMPSSQSTVSEAASAQMSWADMGEEDGLEEEDQKENELGSH 96

Query: 169 VVNVNASTGKLTISKIKPKLSREQREYIRFMNVGRKKDFICLERFKGKLVNILEGLELHT 228
            V+V+ S G    +  K KLSRE+RE  RFMNV + K F C E+ +G+ VNILEGLELHT
Sbjct: 97  GVDVSPSVGDSMKTPEKRKLSREERERYRFMNVKKMKVFSCYEKVRGRSVNILEGLELHT 156

Query: 229 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 288
           G+FSA EQK+IVD VY LQE G++GELRERTF+AP KWM+GKGRVT+QFGCCYNYAPDK 
Sbjct: 157 GVFSAVEQKKIVDFVYELQEKGRRGELRERTFTAPHKWMRGKGRVTIQFGCCYNYAPDKA 216

Query: 289 GNPPGVLRNEIVDPIPSLFKVIIRRMVRWHVLPPTCVPDSCIVNIYEEGDCIPPHIDNHD 348
           GNPPG+L+   VDP+PS+FKVII+R+V WHVLPPTCVPDSCIVNIYEE DCIPPHIDNHD
Sbjct: 217 GNPPGILQRGDVDPMPSIFKVIIKRLVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHD 276

Query: 349 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 408
           F+RPFCTVSFLSECNI+FGSNL ++GPGEFSG  +IPLPVGSVLVL GNGADVAKHCVPA
Sbjct: 277 FLRPFCTVSFLSECNILFGSNLKVLGPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPA 336

Query: 409 VPTKRISITFRRIDDSKRPFEYASEPDLQGIQPLPYEVSENDVPTSPV-SSEREIRRQPF 468
           VPTKRISITFR++D+SKRP  +  EPDL+ I+PLPYE +    P   V SS R    Q  
Sbjct: 337 VPTKRISITFRKMDESKRPVGFTPEPDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNG 396

Query: 469 TRGSHMRTRGSGNR--------SDTRFEPRNSGR----AQHRPADNRRSRV 504
           +  ++    G G++        S++R E  +SG+    ++H P  + R +V
Sbjct: 397 SNHNNRTAHGGGSKYRRSRDYHSESR-ERSSSGQRRETSRHTPNRSYRPKV 446

The following BLAST results are available for this feature:

BLAST of MS010594 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133128.1	8.8e-289	99.60	uncharacterized protein LOC111005802 [Momordica charantia]	[more]
XP_038882153.1	7.8e-253	88.02	RNA demethylase ALKBH9B [Benincasa hispida]	[more]
XP_023518278.1	6.2e-250	86.79	uncharacterized protein LOC111781804 [Cucurbita pepo subsp. pepo]	[more]
XP_023544551.1	2.6e-248	86.93	uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552....	[more]
XP_023003874.1	1.1e-246	86.19	uncharacterized protein LOC111497336 [Cucurbita maxima]	[more]

BLAST of MS010594 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SL49	1.2e-155	58.94	RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1	[more]
Q9ZT92	3.0e-29	32.26	RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1	[more]
Q66JG8	1.9e-12	33.87	RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1	[more]

BLAST of MS010594 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BY69	4.3e-289	99.60	uncharacterized protein LOC111005802 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1KUK5	5.3e-247	86.19	uncharacterized protein LOC111497336 OS=Cucurbita maxima OX=3661 GN=LOC111497336...	[more]
A0A6J1EJB3	5.3e-247	86.19	uncharacterized protein LOC111433825 OS=Cucurbita moschata OX=3662 GN=LOC1114338...	[more]
A0A6J1IMQ3	1.5e-246	86.36	RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1	[more]
A0A6J1GGD7	2.6e-246	86.79	uncharacterized protein LOC111453671 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of MS010594 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G17970.1	8.6e-157	58.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G36090.3	4.0e-146	56.36	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]
AT2G17970.2	2.6e-137	59.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G17970.3	2.6e-137	59.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G36090.2	1.7e-128	59.85	oxidoreductase, 2OG-Fe(II) oxygenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027450	Alpha-ketoglutarate-dependent dioxygenase AlkB-like	PFAM	PF13532	2OG-FeII_Oxy_2	coord: 221..416 e-value: 4.6E-14 score: 53.1
IPR037151	Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamily	GENE3D	2.60.120.590		coord: 205..425 e-value: 5.1E-74 score: 250.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 473..503
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 436..503
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 123..144
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 62..76
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 115..159
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 58..96
None	No IPR available	PANTHER	PTHR31447:SF5	RNA DEMETHYLASE ALKBH9B	coord: 5..500
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 217..418
IPR044842	RNA demethylase ALKBH9B/ALKBH10B-like	PANTHER	PTHR31447	HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATED	coord: 5..500
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 322..419 score: 10.205251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010594.1	MS010594.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006402	mRNA catabolic process
molecular_function	GO:0032451	demethylase activity
molecular_function	GO:0003729	mRNA binding