MS010565 (gene) Bitter gourd (TR) v1

Overview
NameMS010565
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionAAA-ATPase
Locationscaffold35: 600347 .. 601827 (+)
RNA-Seq ExpressionMS010565
SyntenyMS010565
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGAATTTCAAAGAGATGCCGATTCCCCAATCGGCGTCCGCCGCCTTCTCCGCCTACGCCTCCTTCGCCACTACCATGATGCTCGTCCGCTCGGTAACCAACGAGCTCCTCCCTCCAAAGTTCATCTCCTTCCTCTCTTCAATTTTCTTTTACTTCTTCGGATCCGTTTCTTCCCAGACCAAGTTTGTGATCGAGGAGAGTTCCGGGTTCACCTTCAATCAGGTGTTTCAGGCCGCCGAGGTCTATCTCCGCACCAAAATCAGCCCTTCCACCGACACCCTCAAAGTCAGCAAGACTCCAAGGCAGAAGAAAGTCACGCTCTCCATCGACAAGGACCAAGAAATCACCGATTACTTTGAGAATTTTCGCCTCCAGTGGCGGTTCGTCTGCTCACAAGACGAGCGCAACGGAAATTCCAGAGAGAAGCGCCATTTCGAGCTTATATTCCCCAAGAAATTCAGGGACAGAGTTGTTGACTTCTACTTGCCTTACGTCTTGAGAAGAGCCAAGGAGATTAAAGAGGAGGATAAAGTTGTTAAGATCTACAGCCAAGAATGTCAATTTGATGACGACGGCGGCGGCGGAAATTGGGGATCCATAAATCTGGACCATCCAGCCACGTTTGATACCGTGGCCATGGAGCCCGAGTTGAAGCAATTCATAATCGACGATTTGGATAGGTTTGTAAGGAGGAGAGATTTCTATAAAAAGGTAGGGAAGGCTTGGAAGAGGGGATATTTGTTGTATGGCCCCCCTGGTACGGGCAAATCAAGCTTGATCGCCGCCATGGCTAACTACCTCAAGTTTGATATTTACGATTTGGATCTCTCCAACATTTACAGCAATGCTGATTTGAGGAGAGTGATGTTAGCCACCACAAATCGCTCGATTTTGGTGATTGAGGATATAGATTGCAGCGTGGATATACAGAATCGGCAGAACGAGGAACATTTCGATCGTTCCAGCAGCAAGGTCAGTTTTAGTTTTTTTTTTTTTTTCCCTCTTTCAAGATTTCTGAAATTCCGAATTGAAACGGAAAATTTTATGATTGTTTACAGTTTACCTTGTCGGGGATGCTTAATTTCATCGATGGATTGTGGTCGAGTTGCGGGGATGAGAGAATCATCATCTTCACGACGAACCACAAGGACCGATTGGACTCGGCGCTGCTGCGGCCGGGTCGGATGGATGTTCACATAAACATGTCGTATTGCAGCCCTCACGGGTTCAGGGTTCTGGCCTCCAATTACCTCGGCGAGGAAGCGACTCAGCACCGGCTATACGGAGAAATAGAAGACCTAATCAGAGACATGGAGGTATCTCCGGCGGAGATCGCGGAGGAGCTAATGAAAAGCGACGACGCGGACGCCGTTCTCCAAGGTCTTGTCGAGTTTCTGAAACGAAAGAAGGAAGAACAGCGTAAGGCGAAGGAGGAGAAGGCAAGTGAAAAGAACGAAGAGAAAGAGGAAAAAGAGGGG

mRNA sequence

ATGTTGAATTTCAAAGAGATGCCGATTCCCCAATCGGCGTCCGCCGCCTTCTCCGCCTACGCCTCCTTCGCCACTACCATGATGCTCGTCCGCTCGGTAACCAACGAGCTCCTCCCTCCAAAGTTCATCTCCTTCCTCTCTTCAATTTTCTTTTACTTCTTCGGATCCGTTTCTTCCCAGACCAAGTTTGTGATCGAGGAGAGTTCCGGGTTCACCTTCAATCAGGTGTTTCAGGCCGCCGAGGTCTATCTCCGCACCAAAATCAGCCCTTCCACCGACACCCTCAAAGTCAGCAAGACTCCAAGGCAGAAGAAAGTCACGCTCTCCATCGACAAGGACCAAGAAATCACCGATTACTTTGAGAATTTTCGCCTCCAGTGGCGGTTCGTCTGCTCACAAGACGAGCGCAACGGAAATTCCAGAGAGAAGCGCCATTTCGAGCTTATATTCCCCAAGAAATTCAGGGACAGAGTTGTTGACTTCTACTTGCCTTACGTCTTGAGAAGAGCCAAGGAGATTAAAGAGGAGGATAAAGTTGTTAAGATCTACAGCCAAGAATGTCAATTTGATGACGACGGCGGCGGCGGAAATTGGGGATCCATAAATCTGGACCATCCAGCCACGTTTGATACCGTGGCCATGGAGCCCGAGTTGAAGCAATTCATAATCGACGATTTGGATAGGTTTGTAAGGAGGAGAGATTTCTATAAAAAGGTAGGGAAGGCTTGGAAGAGGGGATATTTGTTGTATGGCCCCCCTGGTACGGGCAAATCAAGCTTGATCGCCGCCATGGCTAACTACCTCAAGTTTGATATTTACGATTTGGATCTCTCCAACATTTACAGCAATGCTGATTTGAGGAGAGTGATGTTAGCCACCACAAATCGCTCGATTTTGGTGATTGAGGATATAGATTGCAGCGTGGATATACAGAATCGGCAGAACGAGGAACATTTCGATCGTTCCAGCAGCAAGGTCAGTTTTAGTTTTTTTTTTTTTTTCCCTCTTTCAAGATTTCTGAAATTCCGAATTGAAACGGAAAATTTTATGATTCAGTTTACCTTGTCGGGGATGCTTAATTTCATCGATGGATTGTGGTCGAGTTGCGGGGATGAGAGAATCATCATCTTCACGACGAACCACAAGGACCGATTGGACTCGGCGCTGCTGCGGCCGGGTCGGATGGATGTTCACATAAACATGTCGTATTGCAGCCCTCACGGGTTCAGGGTTCTGGCCTCCAATTACCTCGGCGAGGAAGCGACTCAGCACCGGCTATACGGAGAAATAGAAGACCTAATCAGAGACATGGAGGTATCTCCGGCGGAGATCGCGGAGGAGCTAATGAAAAGCGACGACGCGGACGCCGTTCTCCAAGGTCTTGTCGAGTTTCTGAAACGAAAGAAGGAAGAACAGCGTAAGGCGAAGGAGGAGAAGGCAAGTGAAAAGAACGAAGAGAAAGAGGAAAAAGAGGGG

Coding sequence (CDS)

ATGTTGAATTTCAAAGAGATGCCGATTCCCCAATCGGCGTCCGCCGCCTTCTCCGCCTACGCCTCCTTCGCCACTACCATGATGCTCGTCCGCTCGGTAACCAACGAGCTCCTCCCTCCAAAGTTCATCTCCTTCCTCTCTTCAATTTTCTTTTACTTCTTCGGATCCGTTTCTTCCCAGACCAAGTTTGTGATCGAGGAGAGTTCCGGGTTCACCTTCAATCAGGTGTTTCAGGCCGCCGAGGTCTATCTCCGCACCAAAATCAGCCCTTCCACCGACACCCTCAAAGTCAGCAAGACTCCAAGGCAGAAGAAAGTCACGCTCTCCATCGACAAGGACCAAGAAATCACCGATTACTTTGAGAATTTTCGCCTCCAGTGGCGGTTCGTCTGCTCACAAGACGAGCGCAACGGAAATTCCAGAGAGAAGCGCCATTTCGAGCTTATATTCCCCAAGAAATTCAGGGACAGAGTTGTTGACTTCTACTTGCCTTACGTCTTGAGAAGAGCCAAGGAGATTAAAGAGGAGGATAAAGTTGTTAAGATCTACAGCCAAGAATGTCAATTTGATGACGACGGCGGCGGCGGAAATTGGGGATCCATAAATCTGGACCATCCAGCCACGTTTGATACCGTGGCCATGGAGCCCGAGTTGAAGCAATTCATAATCGACGATTTGGATAGGTTTGTAAGGAGGAGAGATTTCTATAAAAAGGTAGGGAAGGCTTGGAAGAGGGGATATTTGTTGTATGGCCCCCCTGGTACGGGCAAATCAAGCTTGATCGCCGCCATGGCTAACTACCTCAAGTTTGATATTTACGATTTGGATCTCTCCAACATTTACAGCAATGCTGATTTGAGGAGAGTGATGTTAGCCACCACAAATCGCTCGATTTTGGTGATTGAGGATATAGATTGCAGCGTGGATATACAGAATCGGCAGAACGAGGAACATTTCGATCGTTCCAGCAGCAAGGTCAGTTTTAGTTTTTTTTTTTTTTTCCCTCTTTCAAGATTTCTGAAATTCCGAATTGAAACGGAAAATTTTATGATTCAGTTTACCTTGTCGGGGATGCTTAATTTCATCGATGGATTGTGGTCGAGTTGCGGGGATGAGAGAATCATCATCTTCACGACGAACCACAAGGACCGATTGGACTCGGCGCTGCTGCGGCCGGGTCGGATGGATGTTCACATAAACATGTCGTATTGCAGCCCTCACGGGTTCAGGGTTCTGGCCTCCAATTACCTCGGCGAGGAAGCGACTCAGCACCGGCTATACGGAGAAATAGAAGACCTAATCAGAGACATGGAGGTATCTCCGGCGGAGATCGCGGAGGAGCTAATGAAAAGCGACGACGCGGACGCCGTTCTCCAAGGTCTTGTCGAGTTTCTGAAACGAAAGAAGGAAGAACAGCGTAAGGCGAAGGAGGAGAAGGCAAGTGAAAAGAACGAAGAGAAAGAGGAAAAAGAGGGG

Protein sequence

MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKASEKNEEKEEKEG
Homology
BLAST of MS010565 vs. NCBI nr
Match: XP_022132942.1 (AAA-ATPase At2g18193-like, partial [Momordica charantia])

HSP 1 Score: 891.3 bits (2302), Expect = 3.7e-255
Identity = 465/492 (94.51%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ
Sbjct: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
           TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF
Sbjct: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120

Query: 121 ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 180
           ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV
Sbjct: 121 ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 180

Query: 181 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 240
           KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG
Sbjct: 181 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 240

Query: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 300
           KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV
Sbjct: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 300

Query: 301 IEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLN 360
           IEDIDCSVDIQNRQNEEHFDRSSSK                           FTLSGMLN
Sbjct: 301 IEDIDCSVDIQNRQNEEHFDRSSSK---------------------------FTLSGMLN 360

Query: 361 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 420
           FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE
Sbjct: 361 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 420

Query: 421 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 480
           ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA
Sbjct: 421 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 465

Query: 481 SEKNEEKEEKEG 493
           SEKNEEKEEKEG
Sbjct: 481 SEKNEEKEEKEG 465

BLAST of MS010565 vs. NCBI nr
Match: XP_038877872.1 (AAA-ATPase At2g18193-like [Benincasa hispida])

HSP 1 Score: 754.6 bits (1947), Expect = 5.4e-214
Identity = 394/492 (80.08%), Postives = 428/492 (86.99%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           M NFKEM +PQSASA FSAYASFATTMML+RSVTNELLP KFISFLSSIF YFFGS++SQ
Sbjct: 1   MFNFKEMAVPQSASAVFSAYASFATTMMLIRSVTNELLPAKFISFLSSIFVYFFGSIASQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
           TKFVIEE+SGFT N+VFQAAE YLRTKISPS DTLKVSKTPRQKKV LSIDKDQEI DYF
Sbjct: 61  TKFVIEENSGFTINEVFQAAEFYLRTKISPSIDTLKVSKTPRQKKVRLSIDKDQEIIDYF 120

Query: 121 ENFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKV 180
           EN RLQWRFVCS DERN G  REKRHFEL FPKKFRDRVVDFYLPYVL+RAKEIKEE KV
Sbjct: 121 ENIRLQWRFVCSVDERNGGGGREKRHFELSFPKKFRDRVVDFYLPYVLKRAKEIKEEHKV 180

Query: 181 VKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKV 240
           VKI+SQECQ+DDD  GGNWGS+NL+HPATFDT+AM+PELKQ IIDDL+RFVRR+DFYKKV
Sbjct: 181 VKIFSQECQYDDD-SGGNWGSVNLEHPATFDTLAMDPELKQSIIDDLNRFVRRKDFYKKV 240

Query: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSIL 300
           GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDL+N+YSN+DLRRV+LATTNRSIL
Sbjct: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLTNMYSNSDLRRVLLATTNRSIL 300

Query: 301 VIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGML 360
           VIEDIDCSV+IQNRQ+EEHFDRS+SK                           FTLSGML
Sbjct: 301 VIEDIDCSVEIQNRQSEEHFDRSNSK---------------------------FTLSGML 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGE 420
           NFIDGLWSSCGDERIIIFTTNHKDRLD ALLRPGRMDVHINMSYCS  G +VL SNYLG 
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDVHINMSYCSREGLKVLVSNYLGG 420

Query: 421 EATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEK 480
           EAT+H +YGEIE+L+ DMEVSPAEIAEELMK ++ +AVL GLV FLKRK+EEQRK KEEK
Sbjct: 421 EATKHCMYGEIEELMEDMEVSPAEIAEELMKGEETEAVLGGLVGFLKRKREEQRKEKEEK 464

Query: 481 ASEKNEEKEEKE 492
             E+ EE++E E
Sbjct: 481 NEEEEEERDEIE 464

BLAST of MS010565 vs. NCBI nr
Match: XP_022963281.1 (AAA-ATPase At2g18193-like [Cucurbita moschata])

HSP 1 Score: 737.3 bits (1902), Expect = 8.8e-209
Identity = 382/493 (77.48%), Postives = 426/493 (86.41%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEM +PQSASA FSAYASFATTMMLVRS+T+ELLPPK ISF  SIFFYFFGSVS Q
Sbjct: 1   MLNFKEMAVPQSASAVFSAYASFATTMMLVRSITSELLPPKIISFFHSIFFYFFGSVSFQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
            K +IEE+ GF+ NQ+FQAAEVYLRTKISPST+TLK  KT RQKK+TLSIDK QEI D+F
Sbjct: 61  VKIIIEENCGFSPNQIFQAAEVYLRTKISPSTETLKAHKTSRQKKITLSIDKGQEIVDHF 120

Query: 121 ENFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKV 180
           +N RLQWRFVCS DE+N G ++EKRHFEL+FPKKFRDRVV+ YLPYVL+RAKEIKEEDKV
Sbjct: 121 DNIRLQWRFVCSHDEQNGGGNKEKRHFELVFPKKFRDRVVESYLPYVLKRAKEIKEEDKV 180

Query: 181 VKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKV 240
           VKI+SQECQ+D+DGG GNWGSINLDHP+TF+T+AM+P+LKQ IIDDLDRFV RR+FYKKV
Sbjct: 181 VKIFSQECQYDEDGGSGNWGSINLDHPSTFETLAMDPDLKQSIIDDLDRFVNRREFYKKV 240

Query: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSIL 300
           GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLS IYSN DLRRV+L+TTNRSIL
Sbjct: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSYIYSNGDLRRVLLSTTNRSIL 300

Query: 301 VIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGML 360
           VIEDIDCSV+IQNR+NEEH+D+S+ K                           FTLSGML
Sbjct: 301 VIEDIDCSVEIQNRENEEHYDQSNGK---------------------------FTLSGML 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGE 420
           NFIDGLWSSCGDERIIIFTTNHK+RLD ALLRPGRMDVHINMSYC+PHGFRVL +NYLGE
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCTPHGFRVLVTNYLGE 420

Query: 421 EATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEE- 480
           EA +H L  EIE+LI +MEVSPAEIAE LMK+DDAD VLQ LVEFLK K+EEQRK +EE 
Sbjct: 421 EADKHDLCREIEELIGEMEVSPAEIAEALMKNDDADVVLQSLVEFLKCKREEQRKKEEET 466

Query: 481 KASEKNEEKEEKE 492
            ASEKNEE+EE+E
Sbjct: 481 AASEKNEEEEEEE 466

BLAST of MS010565 vs. NCBI nr
Match: XP_023517745.1 (AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 736.9 bits (1901), Expect = 1.2e-208
Identity = 382/494 (77.33%), Postives = 425/494 (86.03%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEM +PQSASA FSAYASFATTMMLVRS+T+ELLPPK ISF  SIFFYFFGSVS Q
Sbjct: 1   MLNFKEMAVPQSASAVFSAYASFATTMMLVRSITSELLPPKIISFFHSIFFYFFGSVSFQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
            K +IEE+ GF+ NQ+FQAAEVYLRTKISPSTDTLK  KTPRQKK+TLSIDK QEI D+F
Sbjct: 61  VKIIIEENCGFSPNQIFQAAEVYLRTKISPSTDTLKAHKTPRQKKITLSIDKGQEIVDHF 120

Query: 121 ENFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKV 180
           +N RLQWRFVCS DE+N G ++EKRHFEL+FPKK RDRVV+ YLPYVL+RAKEIKEEDKV
Sbjct: 121 DNIRLQWRFVCSHDEQNGGGNKEKRHFELVFPKKLRDRVVESYLPYVLKRAKEIKEEDKV 180

Query: 181 VKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKV 240
           VKI+SQECQ+D+DGG GNWGSINLDHP+TF+T+AM+P+LKQ IIDDLDRFV RR+FYKKV
Sbjct: 181 VKIFSQECQYDEDGGSGNWGSINLDHPSTFETLAMDPDLKQSIIDDLDRFVNRREFYKKV 240

Query: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSIL 300
           GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLS IYSN +LRRV+L TTNRSI+
Sbjct: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSYIYSNGELRRVLLTTTNRSIV 300

Query: 301 VIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGML 360
           VIEDIDCSV+IQNR+NEEH+D+S+ K                           FTLSGML
Sbjct: 301 VIEDIDCSVEIQNRENEEHYDQSNGK---------------------------FTLSGML 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGE 420
           NFIDGLWSSCGDERIIIFTTNHK+RLD ALLRPGRMDVHINMSYC+PHGFRVL SNYLGE
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCTPHGFRVLVSNYLGE 420

Query: 421 EATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEK 480
           EA +H L  EIE+LI +MEVSPAEIAE LMK+DDAD VLQ LVEFLK K+EEQRK KEE+
Sbjct: 421 EADKHDLCREIEELIGEMEVSPAEIAEALMKNDDADVVLQSLVEFLKCKREEQRKKKEEE 467

Query: 481 --ASEKNEEKEEKE 492
             ASEKN E+EE+E
Sbjct: 481 TAASEKNGEEEEEE 467

BLAST of MS010565 vs. NCBI nr
Match: XP_008440759.1 (PREDICTED: AAA-ATPase At2g18193-like [Cucumis melo])

HSP 1 Score: 734.6 bits (1895), Expect = 5.7e-208
Identity = 386/496 (77.82%), Postives = 426/496 (85.89%), Query Frame = 0

Query: 2   LNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQT 61
           +NFK+M +PQS SA FSAYASFATTMML+RSVTNELLP K ISFLSSIF YFFGS+SSQT
Sbjct: 1   MNFKDMAVPQSVSAVFSAYASFATTMMLIRSVTNELLPAKLISFLSSIFVYFFGSISSQT 60

Query: 62  KFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFE 121
           KFVIEE+SGF  N+VFQAAE YLRTKISPS DTLKV+KTPRQKKVTLSIDKDQEI DYFE
Sbjct: 61  KFVIEENSGFAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 120

Query: 122 NFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 181
           N RLQWRF+CS DERN G  REKR FEL+FPKKFRDRVVDFYLPYVL+RAKEIKEE+KVV
Sbjct: 121 NIRLQWRFLCSVDERNGGGGREKRQFELLFPKKFRDRVVDFYLPYVLKRAKEIKEENKVV 180

Query: 182 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 241
           KI+SQECQ+DDD  GGNWGS+NL+HPATFDT+AM+PELKQ II+DLDRFVRR+DFYKKVG
Sbjct: 181 KIFSQECQYDDD-SGGNWGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKKVG 240

Query: 242 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 301
           KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSN+YSN+DLRRV+LATTNRSILV
Sbjct: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNMYSNSDLRRVLLATTNRSILV 300

Query: 302 IEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLN 361
           IEDIDCSV+IQNRQ+EE+FDRSSSK                           FTLSGMLN
Sbjct: 301 IEDIDCSVEIQNRQSEENFDRSSSK---------------------------FTLSGMLN 360

Query: 362 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 421
           FIDGLWSSCGDERIIIFTTN+K RLD ALLR GRMD+HINMSYCS  G +VL SNYLG E
Sbjct: 361 FIDGLWSSCGDERIIIFTTNNKHRLDPALLRAGRMDMHINMSYCSREGLKVLVSNYLGGE 420

Query: 422 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 481
           AT+H  YGEIE+LI +MEV+PAEIAEELMK ++ +AVL GL+ FLKRK+EEQRK KEEK 
Sbjct: 421 ATKHSTYGEIEELIGEMEVAPAEIAEELMKGEETEAVLGGLLGFLKRKREEQRKEKEEKK 468

Query: 482 SEKNEE----KEEKEG 493
            EK EE    +EE+EG
Sbjct: 481 EEKGEEEDKIEEEEEG 468

BLAST of MS010565 vs. ExPASy Swiss-Prot
Match: Q8GW96 (AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 1.5e-134
Identity = 257/483 (53.21%), Postives = 345/483 (71.43%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           S S+ FSAYAS    +ML RS+ ++ +P K  S+ SS+   FF   S     +I+E+ G 
Sbjct: 10  SPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKSKYLTVIIDENFGL 69

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             NQVF AAE+YLR+KI P T+ L+V K P+QK  T+SI++ +EI D FE   ++W +V 
Sbjct: 70  NRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWSYVQ 129

Query: 132 SQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVKIYSQE--CQF 191
           S++E+    + KR++EL F KK RD+V++ YL +V+  ++EIK   +VVK+YS++     
Sbjct: 130 SENEK--GDKVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSRDVYASD 189

Query: 192 DDDG-GGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGKAWKRGYL 251
           DDDG  GGNWG INL+HP+TFDT+AM+P  K+ IIDDL+RF++R++FYK+VGKAWKRGYL
Sbjct: 190 DDDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWKRGYL 249

Query: 252 LYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVIEDIDCSV 311
           LYGPPGTGKSSLIAAMANYLKFD++DL+LS+IY N +L+RV+L+TTNRSILVIEDIDC+ 
Sbjct: 250 LYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDIDCNA 309

Query: 312 DIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNFIDGLWSS 371
           ++++R+ E                            E E    + TLSG+LNFIDGLWSS
Sbjct: 310 EVRDREAENQ--------------------------EDEQIKGKVTLSGILNFIDGLWSS 369

Query: 372 CGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQHRLYG 431
            GDERII+FTTNHK+RLD ALLRPGRMDVHINMSYC+  GFR L SNYLG +   H L  
Sbjct: 370 FGDERIIVFTTNHKERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCE 429

Query: 432 EIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKASEKNEEKE 491
           EIE L+   EV+PAE+AEELM+ DD D VL+G++ F++++K E+ K K+E +  K  + +
Sbjct: 430 EIEALVDSTEVTPAELAEELMQDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDD 464

BLAST of MS010565 vs. ExPASy Swiss-Prot
Match: F4IQG2 (AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 5.9e-131
Identity = 253/477 (53.04%), Postives = 335/477 (70.23%), Query Frame = 0

Query: 9   IPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEES 68
           I  S S+ F+AYAS    +ML RS+ N+ +P +  S+++ +   FF   S     VI+E 
Sbjct: 8   ISLSPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKSKNLTMVIDEI 67

Query: 69  SGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWR 128
            GF  NQVF AAEVYLR KI P T  L+V K P+QK  T+ I+K +EI D FEN  L+W 
Sbjct: 68  IGFKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWT 127

Query: 129 FVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVKIYSQECQ 188
           +V S++E   + +EKR++EL F KK RD+V++ YL +V+  ++E K + + VK+YS++ +
Sbjct: 128 YVESENE--ASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVR 187

Query: 189 F--DDDG-GGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGKAWKR 248
              DDDG  G  WG INL+HP+TF+T+AM+P  K+ IIDD++RF++RR+FYK+VGKAWKR
Sbjct: 188 ASKDDDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKR 247

Query: 249 GYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVIEDID 308
           GYLLYGPPGTGKSSLIAAMANYLKFD++DL+LS+IY NA L+ ++L+TTNRSILVIEDID
Sbjct: 248 GYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDID 307

Query: 309 C-SVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNFIDG 368
           C S ++ +R+ +E+ +                          E +  + TLSG+LNF+DG
Sbjct: 308 CSSAEVVDREADEYQEYE------------------------EGYYGRVTLSGLLNFVDG 367

Query: 369 LWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQH 428
           LWSS GDERII+FTTNHK+RLD ALLRPGRMD+HINMSYC+  GFR L SNYLG     H
Sbjct: 368 LWSSFGDERIIVFTTNHKERLDPALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNH 427

Query: 429 RLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKAS 482
            L  EIE LI   EV+PAE+AEELM+ DD D VL+G+V F++ +K E  K KE + S
Sbjct: 428 PLCEEIEALIDSTEVTPAELAEELMQEDDTDVVLRGVVSFVENRKVEISKTKELEGS 458

BLAST of MS010565 vs. ExPASy Swiss-Prot
Match: Q147F9 (AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.9e-121
Identity = 232/469 (49.47%), Postives = 321/469 (68.44%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           +A  A +A AS A   +L RSV  + +P +   ++S  F  FF   S Q   VIEE  GF
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFFSYFSYQMTAVIEEFGGF 70

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             NQVF+AAE YL TKIS ST  +KV+K  +Q   ++++++D+E+ D F+  +L W  VC
Sbjct: 71  EHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILVC 130

Query: 132 ----SQDERNGN------SREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVK 191
                +D RN          E R +EL F KKF++ V++ YLP+V+ +A  IK++ K +K
Sbjct: 131 RHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTLK 190

Query: 192 IYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGK 251
           I      F  D     W S+ LDHP+TF T+A++PE+K+ +++DLDRFV+R+ FY +VGK
Sbjct: 191 I------FTVDSYSVEWTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYGRVGK 250

Query: 252 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVI 311
           AWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDL+++ +NA+LRR++++T NRSILV+
Sbjct: 251 AWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANRSILVV 310

Query: 312 EDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNF 371
           EDIDCS+++++R  ++  +    K                            TLSG+LNF
Sbjct: 311 EDIDCSIELKDRSTDQENNDPLHKT--------------------------VTLSGLLNF 370

Query: 372 IDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEA 431
           +DGLWSSCG+ERII+FTTN++++LD ALLRPGRMD+HI+MSYC+P  F+VLASNYL  E 
Sbjct: 371 VDGLWSSCGNERIIVFTTNYREKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EI 430

Query: 432 TQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKE 471
             H L+ +IE+ IR++EV+PAE+AE+LM+SD  D VLQGLVEFLK KK+
Sbjct: 431 QDHILFEQIEEFIREIEVTPAEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MS010565 vs. ExPASy Swiss-Prot
Match: Q8VZG2 (Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 4.7e-120
Identity = 243/493 (49.29%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           +A    +  AS A T ML RS+  + LP +   ++S  F   FG  SSQ   +IEE  GF
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYFSSQMTIIIEEFEGF 116

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             N+VF+AAE YL TKISPS   +KVSK  ++    +++++D+E+ D +   + QW   C
Sbjct: 117 AHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILHC 176

Query: 132 SQDE-------RNGNS---REKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVK 191
              E       R+ NS    E R FEL F KKF+D  ++ YLP++++RA  +K+E K +K
Sbjct: 177 RHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTLK 236

Query: 192 IYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGK 251
           I++   +         W S+ LDHP+TF T+AM+ ++K  +++DLD+FV+RRDFYK+VGK
Sbjct: 237 IFTLSPENMYGNYSDAWTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRRDFYKRVGK 296

Query: 252 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVI 311
           AWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+L+ + +N++LRR+++AT NRSIL++
Sbjct: 297 AWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIATANRSILIV 356

Query: 312 EDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNF 371
           EDIDCS+++++R ++E    S                     IE   +  + TLSG+LNF
Sbjct: 357 EDIDCSLELKDRTSDEPPRESDD-------------------IEDPRYK-KVTLSGLLNF 416

Query: 372 IDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEA 431
           IDGLWSSCGDERIIIFTTN+K++LD+ALLRPGRMD+HI+MSYC+P  F+ LA NYL  E 
Sbjct: 417 IDGLWSSCGDERIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EI 476

Query: 432 TQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKK--EEQRKAKEEK 491
            +HRL+ +IE+ I   EV+PAE+AE+LM++D  D VL+GL+EFLK KK   EQ KAK EK
Sbjct: 477 KEHRLFSKIEEGIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEK 526

Query: 492 ASEKNEEKEEKEG 493
             E   +K+ KEG
Sbjct: 537 -QELENKKKTKEG 526

BLAST of MS010565 vs. ExPASy Swiss-Prot
Match: Q9FN75 (AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.5e-114
Identity = 237/511 (46.38%), Postives = 331/511 (64.77%), Query Frame = 0

Query: 5   KEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFL-SSIFFYFFGSVSSQTKF 64
           K++P P S    F+AYAS A  MM++RS+ +EL+P     F+  ++   FF S SS    
Sbjct: 5   KDLPSPTS---VFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTLTL 64

Query: 65  VIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENF 124
            I++ +    N++++AA+ YL TKISP    L++SK  + K V L +   + + D +E+ 
Sbjct: 65  TIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYEDV 124

Query: 125 RLQWRFVCSQDERNG-------------------NSREKRHFELIFPKKFRDRVVDFYLP 184
           +L WRFV    ++ G                   +  +  +FEL F KK +D +++ Y+P
Sbjct: 125 QLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEYFELSFDKKHKDLILNSYVP 184

Query: 185 YVLRRAKEIKEEDKVVKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIID 244
           Y+  +AKEI++E +++ ++S             W S+ L+HP+TF+T+AME +LK+ +I+
Sbjct: 185 YIESKAKEIRDERRILMLHSLNSL--------RWESVILEHPSTFETMAMEDDLKRDVIE 244

Query: 245 DLDRFVRRRDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNA 304
           DLDRF+RR++FYK+VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL L+++  ++
Sbjct: 245 DLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQLASVMRDS 304

Query: 305 DLRRVMLATTNRSILVIEDIDCSVDIQNR--QNEEHFDRSSSKVSFSFFFFFPLSRFLKF 364
           DLRR++LAT NRSILVIEDIDC+VD+ NR  Q  E  +R  S+         PL      
Sbjct: 305 DLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQ--------GPL------ 364

Query: 365 RIETENFMIQFTLSGMLNFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMS 424
                      TLSG+LNFIDGLWSSCGDERIIIFTTNHKDRLD ALLRPGRMD+HI M 
Sbjct: 365 -----------TLSGLLNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMG 424

Query: 425 YCSPHGFRVLASNYLG--EEATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQG 484
           +CS  GF+ LASNYLG  + A  HRL+ EIE LI    ++PA++AEELMKS+DAD  L+G
Sbjct: 425 HCSFQGFKTLASNYLGLSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEG 479

Query: 485 LVEFLKRKKEEQRKAKEEKASEKNEEKEEKE 492
           LV  L++ + + +++      +K    E +E
Sbjct: 485 LVNVLEKMRLKSKESNPVMMKQKESRLEMEE 479

BLAST of MS010565 vs. ExPASy TrEMBL
Match: A0A6J1BV86 (AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005662 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.8e-255
Identity = 465/492 (94.51%), Postives = 465/492 (94.51%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ
Sbjct: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
           TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF
Sbjct: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120

Query: 121 ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 180
           ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV
Sbjct: 121 ENFRLQWRFVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 180

Query: 181 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 240
           KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG
Sbjct: 181 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 240

Query: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 300
           KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV
Sbjct: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 300

Query: 301 IEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLN 360
           IEDIDCSVDIQNRQNEEHFDRSSSK                           FTLSGMLN
Sbjct: 301 IEDIDCSVDIQNRQNEEHFDRSSSK---------------------------FTLSGMLN 360

Query: 361 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 420
           FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE
Sbjct: 361 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 420

Query: 421 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 480
           ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA
Sbjct: 421 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 465

Query: 481 SEKNEEKEEKEG 493
           SEKNEEKEEKEG
Sbjct: 481 SEKNEEKEEKEG 465

BLAST of MS010565 vs. ExPASy TrEMBL
Match: A0A6J1HFQ8 (AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463542 PE=3 SV=1)

HSP 1 Score: 737.3 bits (1902), Expect = 4.3e-209
Identity = 382/493 (77.48%), Postives = 426/493 (86.41%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEM +PQSASA FSAYASFATTMMLVRS+T+ELLPPK ISF  SIFFYFFGSVS Q
Sbjct: 1   MLNFKEMAVPQSASAVFSAYASFATTMMLVRSITSELLPPKIISFFHSIFFYFFGSVSFQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
            K +IEE+ GF+ NQ+FQAAEVYLRTKISPST+TLK  KT RQKK+TLSIDK QEI D+F
Sbjct: 61  VKIIIEENCGFSPNQIFQAAEVYLRTKISPSTETLKAHKTSRQKKITLSIDKGQEIVDHF 120

Query: 121 ENFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKV 180
           +N RLQWRFVCS DE+N G ++EKRHFEL+FPKKFRDRVV+ YLPYVL+RAKEIKEEDKV
Sbjct: 121 DNIRLQWRFVCSHDEQNGGGNKEKRHFELVFPKKFRDRVVESYLPYVLKRAKEIKEEDKV 180

Query: 181 VKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKV 240
           VKI+SQECQ+D+DGG GNWGSINLDHP+TF+T+AM+P+LKQ IIDDLDRFV RR+FYKKV
Sbjct: 181 VKIFSQECQYDEDGGSGNWGSINLDHPSTFETLAMDPDLKQSIIDDLDRFVNRREFYKKV 240

Query: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSIL 300
           GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLS IYSN DLRRV+L+TTNRSIL
Sbjct: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSYIYSNGDLRRVLLSTTNRSIL 300

Query: 301 VIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGML 360
           VIEDIDCSV+IQNR+NEEH+D+S+ K                           FTLSGML
Sbjct: 301 VIEDIDCSVEIQNRENEEHYDQSNGK---------------------------FTLSGML 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGE 420
           NFIDGLWSSCGDERIIIFTTNHK+RLD ALLRPGRMDVHINMSYC+PHGFRVL +NYLGE
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCTPHGFRVLVTNYLGE 420

Query: 421 EATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEE- 480
           EA +H L  EIE+LI +MEVSPAEIAE LMK+DDAD VLQ LVEFLK K+EEQRK +EE 
Sbjct: 421 EADKHDLCREIEELIGEMEVSPAEIAEALMKNDDADVVLQSLVEFLKCKREEQRKKEEET 466

Query: 481 KASEKNEEKEEKE 492
            ASEKNEE+EE+E
Sbjct: 481 AASEKNEEEEEEE 466

BLAST of MS010565 vs. ExPASy TrEMBL
Match: A0A1S3B2H6 (AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485078 PE=3 SV=1)

HSP 1 Score: 734.6 bits (1895), Expect = 2.8e-208
Identity = 386/496 (77.82%), Postives = 426/496 (85.89%), Query Frame = 0

Query: 2   LNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQT 61
           +NFK+M +PQS SA FSAYASFATTMML+RSVTNELLP K ISFLSSIF YFFGS+SSQT
Sbjct: 1   MNFKDMAVPQSVSAVFSAYASFATTMMLIRSVTNELLPAKLISFLSSIFVYFFGSISSQT 60

Query: 62  KFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFE 121
           KFVIEE+SGF  N+VFQAAE YLRTKISPS DTLKV+KTPRQKKVTLSIDKDQEI DYFE
Sbjct: 61  KFVIEENSGFAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 120

Query: 122 NFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 181
           N RLQWRF+CS DERN G  REKR FEL+FPKKFRDRVVDFYLPYVL+RAKEIKEE+KVV
Sbjct: 121 NIRLQWRFLCSVDERNGGGGREKRQFELLFPKKFRDRVVDFYLPYVLKRAKEIKEENKVV 180

Query: 182 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 241
           KI+SQECQ+DDD  GGNWGS+NL+HPATFDT+AM+PELKQ II+DLDRFVRR+DFYKKVG
Sbjct: 181 KIFSQECQYDDD-SGGNWGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKKVG 240

Query: 242 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 301
           KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSN+YSN+DLRRV+LATTNRSILV
Sbjct: 241 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNMYSNSDLRRVLLATTNRSILV 300

Query: 302 IEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLN 361
           IEDIDCSV+IQNRQ+EE+FDRSSSK                           FTLSGMLN
Sbjct: 301 IEDIDCSVEIQNRQSEENFDRSSSK---------------------------FTLSGMLN 360

Query: 362 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 421
           FIDGLWSSCGDERIIIFTTN+K RLD ALLR GRMD+HINMSYCS  G +VL SNYLG E
Sbjct: 361 FIDGLWSSCGDERIIIFTTNNKHRLDPALLRAGRMDMHINMSYCSREGLKVLVSNYLGGE 420

Query: 422 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 481
           AT+H  YGEIE+LI +MEV+PAEIAEELMK ++ +AVL GL+ FLKRK+EEQRK KEEK 
Sbjct: 421 ATKHSTYGEIEELIGEMEVAPAEIAEELMKGEETEAVLGGLLGFLKRKREEQRKEKEEKK 468

Query: 482 SEKNEE----KEEKEG 493
            EK EE    +EE+EG
Sbjct: 481 EEKGEEEDKIEEEEEG 468

BLAST of MS010565 vs. ExPASy TrEMBL
Match: A0A6J1KTM6 (AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497117 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.7e-208
Identity = 381/493 (77.28%), Postives = 425/493 (86.21%), Query Frame = 0

Query: 1   MLNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQ 60
           MLNFKEM +PQSASA FSAYASFATTMMLVRS+T+ELLPPK ISF  SIFFYFFGSVS Q
Sbjct: 1   MLNFKEMAVPQSASAVFSAYASFATTMMLVRSITSELLPPKIISFFHSIFFYFFGSVSFQ 60

Query: 61  TKFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYF 120
            K +IEE+ GF+ NQ+FQAAEVYLRTKISPSTDTLK  KTPRQKK+TLSIDK QEI D+F
Sbjct: 61  VKIIIEENCGFSPNQIFQAAEVYLRTKISPSTDTLKAHKTPRQKKITLSIDKGQEIVDHF 120

Query: 121 ENFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKV 180
           +N RLQWRFVCS DE+N G ++EKRHFEL+FPKKFRDRVV+ YLPYVL+RAKEIKEEDKV
Sbjct: 121 DNIRLQWRFVCSHDEQNGGGNKEKRHFELVFPKKFRDRVVESYLPYVLKRAKEIKEEDKV 180

Query: 181 VKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKV 240
           VKI+SQECQ+D+DGG GNWGSINLDHP+TF+T+AM+P+LKQ IIDDLDRFV RR+FYKKV
Sbjct: 181 VKIFSQECQYDEDGGSGNWGSINLDHPSTFETLAMDPDLKQSIIDDLDRFVNRREFYKKV 240

Query: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSIL 300
           GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLS IYSN DLRRV+L+TTNRSI+
Sbjct: 241 GKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSYIYSNGDLRRVLLSTTNRSIV 300

Query: 301 VIEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGML 360
           VIEDIDCSV+IQNR+NEE +D+S+ K                           FTLSGML
Sbjct: 301 VIEDIDCSVEIQNRENEEQYDQSNGK---------------------------FTLSGML 360

Query: 361 NFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGE 420
           NFIDGLWSSCGDERIIIFTTNHK+RLD ALLRPGRMDVHINMSYC+PHGFRVL SNYLGE
Sbjct: 361 NFIDGLWSSCGDERIIIFTTNHKERLDPALLRPGRMDVHINMSYCTPHGFRVLVSNYLGE 420

Query: 421 EATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEE- 480
           EA +H+L  EIE+LI +MEVSPAEIAE LMK+DDAD VLQ LVEFLK K+EEQRK +EE 
Sbjct: 421 EADKHQLCREIEELIGEMEVSPAEIAEALMKNDDADVVLQRLVEFLKCKREEQRKKEEET 466

Query: 481 KASEKNEEKEEKE 492
            ASEKNE +E +E
Sbjct: 481 AASEKNEGEEGEE 466

BLAST of MS010565 vs. ExPASy TrEMBL
Match: A0A0A0KGJ8 (AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504460 PE=3 SV=1)

HSP 1 Score: 723.0 bits (1865), Expect = 8.4e-205
Identity = 381/491 (77.60%), Postives = 420/491 (85.54%), Query Frame = 0

Query: 2   LNFKEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQT 61
           +NFK+M +PQS SA FSAYASFATTMML+RS+TNELLP K ISF SSIF YFFGS+SSQT
Sbjct: 1   MNFKDMAVPQSVSALFSAYASFATTMMLIRSLTNELLPAKLISF-SSIFVYFFGSISSQT 60

Query: 62  KFVIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFE 121
           K VIEE+SGF  N+VFQAAE YLRTKISPS DTLKV+KTPRQKKVTLSIDKDQEI DYFE
Sbjct: 61  KLVIEENSGFAMNEVFQAAEFYLRTKISPSIDTLKVTKTPRQKKVTLSIDKDQEIIDYFE 120

Query: 122 NFRLQWRFVCSQDERN-GNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVV 181
           N RLQWRF+CS DERN G SREKR FEL FPKKFRDR+VDFYLPYVLRRAKEIKEE+KVV
Sbjct: 121 NIRLQWRFLCSVDERNGGGSREKRQFELSFPKKFRDRIVDFYLPYVLRRAKEIKEENKVV 180

Query: 182 KIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVG 241
           KI+SQECQ+DDD  GGNWGS+NL+HPATFDT+AM+PELKQ II+DLDRFVRR+DFYKKVG
Sbjct: 181 KIFSQECQYDDD-SGGNWGSVNLEHPATFDTLAMDPELKQSIIEDLDRFVRRKDFYKKVG 240

Query: 242 KAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILV 301
           KAWKRGYLL GPPGTGKSSLIAAMANYLKFDIYDLDL+N+YSN+DLRRV+LATTNRSILV
Sbjct: 241 KAWKRGYLLEGPPGTGKSSLIAAMANYLKFDIYDLDLTNMYSNSDLRRVLLATTNRSILV 300

Query: 302 IEDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLN 361
           IEDIDCSV IQNRQ+EEHFD+SSSK                           FTLSGMLN
Sbjct: 301 IEDIDCSVQIQNRQSEEHFDQSSSK---------------------------FTLSGMLN 360

Query: 362 FIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEE 421
           FIDGLWSSCGDERIIIFTTN+K RLD ALLR GRMD+HINMSYCS  G RVL SNYLG E
Sbjct: 361 FIDGLWSSCGDERIIIFTTNNKHRLDPALLRAGRMDMHINMSYCSREGLRVLVSNYLGGE 420

Query: 422 ATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKA 481
           AT+H  YGEIE+LI +MEV+PAEIAEELMK ++ +AVL GLV FLKRK+EE+RK KEEK 
Sbjct: 421 ATKHSTYGEIEELIGEMEVAPAEIAEELMKGEETEAVLGGLVGFLKRKREEERKEKEEKK 462

Query: 482 SEKNEEKEEKE 492
            EK EE+E+ E
Sbjct: 481 EEKGEEEEKVE 462

BLAST of MS010565 vs. TAIR 10
Match: AT2G18193.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 481.1 bits (1237), Expect = 1.1e-135
Identity = 257/483 (53.21%), Postives = 345/483 (71.43%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           S S+ FSAYAS    +ML RS+ ++ +P K  S+ SS+   FF   S     +I+E+ G 
Sbjct: 10  SPSSLFSAYASLTGFLMLFRSMLHDFVPEKLRSYFSSLLDRFFTPKSKYLTVIIDENFGL 69

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             NQVF AAE+YLR+KI P T+ L+V K P+QK  T+SI++ +EI D FE   ++W +V 
Sbjct: 70  NRNQVFDAAEMYLRSKIGPETERLRVGKIPKQKHFTISIERGEEILDTFEESEVKWSYVQ 129

Query: 132 SQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVKIYSQE--CQF 191
           S++E+    + KR++EL F KK RD+V++ YL +V+  ++EIK   +VVK+YS++     
Sbjct: 130 SENEK--GDKVKRYYELTFEKKLRDKVLNSYLTHVVAESEEIKRNLRVVKLYSRDVYASD 189

Query: 192 DDDG-GGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGKAWKRGYL 251
           DDDG  GGNWG INL+HP+TFDT+AM+P  K+ IIDDL+RF++R++FYK+VGKAWKRGYL
Sbjct: 190 DDDGMAGGNWGCINLEHPSTFDTLAMDPNAKKKIIDDLERFLKRKEFYKRVGKAWKRGYL 249

Query: 252 LYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVIEDIDCSV 311
           LYGPPGTGKSSLIAAMANYLKFD++DL+LS+IY N +L+RV+L+TTNRSILVIEDIDC+ 
Sbjct: 250 LYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYDNGELKRVLLSTTNRSILVIEDIDCNA 309

Query: 312 DIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNFIDGLWSS 371
           ++++R+ E                            E E    + TLSG+LNFIDGLWSS
Sbjct: 310 EVRDREAENQ--------------------------EDEQIKGKVTLSGILNFIDGLWSS 369

Query: 372 CGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQHRLYG 431
            GDERII+FTTNHK+RLD ALLRPGRMDVHINMSYC+  GFR L SNYLG +   H L  
Sbjct: 370 FGDERIIVFTTNHKERLDPALLRPGRMDVHINMSYCTGLGFRTLVSNYLGLDGLNHPLCE 429

Query: 432 EIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKASEKNEEKE 491
           EIE L+   EV+PAE+AEELM+ DD D VL+G++ F++++K E+ K K+E +  K  + +
Sbjct: 430 EIEALVDSTEVTPAELAEELMQDDDTDVVLRGVISFVEKRKVERSKTKKEVSICKATDDD 464

BLAST of MS010565 vs. TAIR 10
Match: AT2G18190.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 469.2 bits (1206), Expect = 4.2e-132
Identity = 253/477 (53.04%), Postives = 335/477 (70.23%), Query Frame = 0

Query: 9   IPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEES 68
           I  S S+ F+AYAS    +ML RS+ N+ +P +  S+++ +   FF   S     VI+E 
Sbjct: 8   ISLSPSSLFTAYASLTGFLMLFRSLFNDEVPERLRSYITDLLNRFFTPKSKNLTMVIDEI 67

Query: 69  SGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWR 128
            GF  NQVF AAEVYLR KI P T  L+V K P+QK  T+ I+K +EI D FEN  L+W 
Sbjct: 68  IGFKRNQVFDAAEVYLRNKIGPETARLRVGKLPKQKHFTIYIEKGEEILDTFENSELRWT 127

Query: 129 FVCSQDERNGNSREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVKIYSQECQ 188
           +V S++E   + +EKR++EL F KK RD+V++ YL +V+  ++E K + + VK+YS++ +
Sbjct: 128 YVESENE--ASQKEKRYYELTFEKKLRDKVMNSYLSHVVAESEETKRDLRAVKLYSRDVR 187

Query: 189 F--DDDG-GGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGKAWKR 248
              DDDG  G  WG INL+HP+TF+T+AM+P  K+ IIDD++RF++RR+FYK+VGKAWKR
Sbjct: 188 ASKDDDGMAGAGWGCINLEHPSTFETLAMDPGAKKKIIDDMERFLKRREFYKRVGKAWKR 247

Query: 249 GYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVIEDID 308
           GYLLYGPPGTGKSSLIAAMANYLKFD++DL+LS+IY NA L+ ++L+TTNRSILVIEDID
Sbjct: 248 GYLLYGPPGTGKSSLIAAMANYLKFDVFDLELSSIYENAQLKSILLSTTNRSILVIEDID 307

Query: 309 C-SVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNFIDG 368
           C S ++ +R+ +E+ +                          E +  + TLSG+LNF+DG
Sbjct: 308 CSSAEVVDREADEYQEYE------------------------EGYYGRVTLSGLLNFVDG 367

Query: 369 LWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEATQH 428
           LWSS GDERII+FTTNHK+RLD ALLRPGRMD+HINMSYC+  GFR L SNYLG     H
Sbjct: 368 LWSSFGDERIIVFTTNHKERLDPALLRPGRMDMHINMSYCTGLGFRTLVSNYLGLGGLNH 427

Query: 429 RLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKEEQRKAKEEKAS 482
            L  EIE LI   EV+PAE+AEELM+ DD D VL+G+V F++ +K E  K KE + S
Sbjct: 428 PLCEEIEALIDSTEVTPAELAEELMQEDDTDVVLRGVVSFVENRKVEISKTKELEGS 458

BLAST of MS010565 vs. TAIR 10
Match: AT3G50940.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 437.6 bits (1124), Expect = 1.4e-122
Identity = 232/469 (49.47%), Postives = 321/469 (68.44%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           +A  A +A AS A   +L RSV  + +P +   ++S  F  FF   S Q   VIEE  GF
Sbjct: 11  TAKTALTAVASVAAAAILARSVVQDYMPNEVHEYISHGFRRFFSYFSYQMTAVIEEFGGF 70

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             NQVF+AAE YL TKIS ST  +KV+K  +Q   ++++++D+E+ D F+  +L W  VC
Sbjct: 71  EHNQVFEAAEAYLSTKISNSTRRIKVNKLEKQSNYSVTVERDEEVVDIFDGVKLSWILVC 130

Query: 132 ----SQDERNGN------SREKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVK 191
                +D RN          E R +EL F KKF++ V++ YLP+V+ +A  IK++ K +K
Sbjct: 131 RHVDKKDFRNPRDLNSTLKSEVRSYELSFRKKFKNMVLESYLPFVVEQAASIKQKFKTLK 190

Query: 192 IYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGK 251
           I      F  D     W S+ LDHP+TF T+A++PE+K+ +++DLDRFV+R+ FY +VGK
Sbjct: 191 I------FTVDSYSVEWTSVTLDHPSTFRTLALDPEVKKNLVEDLDRFVQRKGFYGRVGK 250

Query: 252 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVI 311
           AWKRGYLLYGPPGTGKSSLIAA+AN+L FDIYDLDL+++ +NA+LRR++++T NRSILV+
Sbjct: 251 AWKRGYLLYGPPGTGKSSLIAAIANHLNFDIYDLDLTSLNNNAELRRLLMSTANRSILVV 310

Query: 312 EDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNF 371
           EDIDCS+++++R  ++  +    K                            TLSG+LNF
Sbjct: 311 EDIDCSIELKDRSTDQENNDPLHKT--------------------------VTLSGLLNF 370

Query: 372 IDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEA 431
           +DGLWSSCG+ERII+FTTN++++LD ALLRPGRMD+HI+MSYC+P  F+VLASNYL  E 
Sbjct: 371 VDGLWSSCGNERIIVFTTNYREKLDPALLRPGRMDMHIHMSYCTPAAFKVLASNYL--EI 430

Query: 432 TQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKKE 471
             H L+ +IE+ IR++EV+PAE+AE+LM+SD  D VLQGLVEFLK KK+
Sbjct: 431 QDHILFEQIEEFIREIEVTPAEVAEQLMRSDSVDKVLQGLVEFLKAKKQ 445

BLAST of MS010565 vs. TAIR 10
Match: AT3G50930.1 (cytochrome BC1 synthesis )

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-121
Identity = 243/493 (49.29%), Postives = 334/493 (67.75%), Query Frame = 0

Query: 12  SASAAFSAYASFATTMMLVRSVTNELLPPKFISFLSSIFFYFFGSVSSQTKFVIEESSGF 71
           +A    +  AS A T ML RS+  + LP +   ++S  F   FG  SSQ   +IEE  GF
Sbjct: 57  TAKTVLTTAASVAATAMLARSLVQDYLPDEVHHYISYGFRSIFGYFSSQMTIIIEEFEGF 116

Query: 72  TFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENFRLQWRFVC 131
             N+VF+AAE YL TKISPS   +KVSK  ++    +++++D+E+ D +   + QW   C
Sbjct: 117 AHNEVFEAAEAYLATKISPSNKRIKVSKHEKENNYNVTVERDEEVVDTYNGVKFQWILHC 176

Query: 132 SQDE-------RNGNS---REKRHFELIFPKKFRDRVVDFYLPYVLRRAKEIKEEDKVVK 191
              E       R+ NS    E R FEL F KKF+D  ++ YLP++++RA  +K+E K +K
Sbjct: 177 RHVESKHFHNPRDLNSTLRSEVRSFELNFHKKFKDVALESYLPFMVKRATLMKQEKKTLK 236

Query: 192 IYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIIDDLDRFVRRRDFYKKVGK 251
           I++   +         W S+ LDHP+TF T+AM+ ++K  +++DLD+FV+RRDFYK+VGK
Sbjct: 237 IFTLSPENMYGNYSDAWTSVTLDHPSTFKTLAMDSDVKTSVMEDLDKFVKRRDFYKRVGK 296

Query: 252 AWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNADLRRVMLATTNRSILVI 311
           AWKRGYLLYGPPGTGKSSLIAAMAN+L FDIYDL+L+ + +N++LRR+++AT NRSIL++
Sbjct: 297 AWKRGYLLYGPPGTGKSSLIAAMANHLNFDIYDLELTAVNNNSELRRLLIATANRSILIV 356

Query: 312 EDIDCSVDIQNRQNEEHFDRSSSKVSFSFFFFFPLSRFLKFRIETENFMIQFTLSGMLNF 371
           EDIDCS+++++R ++E    S                     IE   +  + TLSG+LNF
Sbjct: 357 EDIDCSLELKDRTSDEPPRESDD-------------------IEDPRYK-KVTLSGLLNF 416

Query: 372 IDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMSYCSPHGFRVLASNYLGEEA 431
           IDGLWSSCGDERIIIFTTN+K++LD+ALLRPGRMD+HI+MSYC+P  F+ LA NYL  E 
Sbjct: 417 IDGLWSSCGDERIIIFTTNYKEKLDAALLRPGRMDMHIHMSYCTPSTFKALALNYL--EI 476

Query: 432 TQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQGLVEFLKRKK--EEQRKAKEEK 491
            +HRL+ +IE+ I   EV+PAE+AE+LM++D  D VL+GL+EFLK KK   EQ KAK EK
Sbjct: 477 KEHRLFSKIEEGIEATEVTPAEVAEQLMRNDSVDKVLEGLIEFLKVKKIENEQDKAKTEK 526

Query: 492 ASEKNEEKEEKEG 493
             E   +K+ KEG
Sbjct: 537 -QELENKKKTKEG 526

BLAST of MS010565 vs. TAIR 10
Match: AT5G17760.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 412.1 bits (1058), Expect = 6.1e-115
Identity = 237/511 (46.38%), Postives = 331/511 (64.77%), Query Frame = 0

Query: 5   KEMPIPQSASAAFSAYASFATTMMLVRSVTNELLPPKFISFL-SSIFFYFFGSVSSQTKF 64
           K++P P S    F+AYAS A  MM++RS+ +EL+P     F+  ++   FF S SS    
Sbjct: 5   KDLPSPTS---VFTAYASMAGYMMMIRSMAHELIPAPLQDFIYRTLRSLFFRSSSSTLTL 64

Query: 65  VIEESSGFTFNQVFQAAEVYLRTKISPSTDTLKVSKTPRQKKVTLSIDKDQEITDYFENF 124
            I++ +    N++++AA+ YL TKISP    L++SK  + K V L +   + + D +E+ 
Sbjct: 65  TIDDDNMGMNNEIYRAAQTYLSTKISPDAVRLRISKGHKDKHVNLYLSDGEIVNDVYEDV 124

Query: 125 RLQWRFVCSQDERNG-------------------NSREKRHFELIFPKKFRDRVVDFYLP 184
           +L WRFV    ++ G                   +  +  +FEL F KK +D +++ Y+P
Sbjct: 125 QLVWRFVTDGGDKKGGGGGVGGRGGGGGRRGGMDDDGKSEYFELSFDKKHKDLILNSYVP 184

Query: 185 YVLRRAKEIKEEDKVVKIYSQECQFDDDGGGGNWGSINLDHPATFDTVAMEPELKQFIID 244
           Y+  +AKEI++E +++ ++S             W S+ L+HP+TF+T+AME +LK+ +I+
Sbjct: 185 YIESKAKEIRDERRILMLHSLNSL--------RWESVILEHPSTFETMAMEDDLKRDVIE 244

Query: 245 DLDRFVRRRDFYKKVGKAWKRGYLLYGPPGTGKSSLIAAMANYLKFDIYDLDLSNIYSNA 304
           DLDRF+RR++FYK+VGKAWKRGYLLYGPPGTGKSSL+AAMANYLKFD+YDL L+++  ++
Sbjct: 245 DLDRFIRRKEFYKRVGKAWKRGYLLYGPPGTGKSSLVAAMANYLKFDVYDLQLASVMRDS 304

Query: 305 DLRRVMLATTNRSILVIEDIDCSVDIQNR--QNEEHFDRSSSKVSFSFFFFFPLSRFLKF 364
           DLRR++LAT NRSILVIEDIDC+VD+ NR  Q  E  +R  S+         PL      
Sbjct: 305 DLRRLLLATRNRSILVIEDIDCAVDLPNRIEQPVEGKNRGESQ--------GPL------ 364

Query: 365 RIETENFMIQFTLSGMLNFIDGLWSSCGDERIIIFTTNHKDRLDSALLRPGRMDVHINMS 424
                      TLSG+LNFIDGLWSSCGDERIIIFTTNHKDRLD ALLRPGRMD+HI M 
Sbjct: 365 -----------TLSGLLNFIDGLWSSCGDERIIIFTTNHKDRLDPALLRPGRMDMHIYMG 424

Query: 425 YCSPHGFRVLASNYLG--EEATQHRLYGEIEDLIRDMEVSPAEIAEELMKSDDADAVLQG 484
           +CS  GF+ LASNYLG  + A  HRL+ EIE LI    ++PA++AEELMKS+DAD  L+G
Sbjct: 425 HCSFQGFKTLASNYLGLSDAAMPHRLFPEIERLIDGEVMTPAQVAEELMKSEDADVALEG 479

Query: 485 LVEFLKRKKEEQRKAKEEKASEKNEEKEEKE 492
           LV  L++ + + +++      +K    E +E
Sbjct: 485 LVNVLEKMRLKSKESNPVMMKQKESRLEMEE 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132942.13.7e-25594.51AAA-ATPase At2g18193-like, partial [Momordica charantia][more]
XP_038877872.15.4e-21480.08AAA-ATPase At2g18193-like [Benincasa hispida][more]
XP_022963281.18.8e-20977.48AAA-ATPase At2g18193-like [Cucurbita moschata][more]
XP_023517745.11.2e-20877.33AAA-ATPase At2g18193-like [Cucurbita pepo subsp. pepo][more]
XP_008440759.15.7e-20877.82PREDICTED: AAA-ATPase At2g18193-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
Q8GW961.5e-13453.21AAA-ATPase At2g18193 OS=Arabidopsis thaliana OX=3702 GN=At2g18193 PE=2 SV=1[more]
F4IQG25.9e-13153.04AAA-ATPase At2g18190 OS=Arabidopsis thaliana OX=3702 GN=At2g18190 PE=2 SV=1[more]
Q147F91.9e-12149.47AAA-ATPase At3g50940 OS=Arabidopsis thaliana OX=3702 GN=At3g50940 PE=2 SV=1[more]
Q8VZG24.7e-12049.29Protein HYPER-SENSITIVITY-RELATED 4 OS=Arabidopsis thaliana OX=3702 GN=HSR4 PE=2... [more]
Q9FN758.5e-11446.38AAA-ATPase At5g17760 OS=Arabidopsis thaliana OX=3702 GN=At5g17760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BV861.8e-25594.51AAA-ATPase At2g18193-like OS=Momordica charantia OX=3673 GN=LOC111005662 PE=3 SV... [more]
A0A6J1HFQ84.3e-20977.48AAA-ATPase At2g18193-like OS=Cucurbita moschata OX=3662 GN=LOC111463542 PE=3 SV=... [more]
A0A1S3B2H62.8e-20877.82AAA-ATPase At2g18193-like OS=Cucumis melo OX=3656 GN=LOC103485078 PE=3 SV=1[more]
A0A6J1KTM64.7e-20877.28AAA-ATPase At2g18193-like OS=Cucurbita maxima OX=3661 GN=LOC111497117 PE=3 SV=1[more]
A0A0A0KGJ88.4e-20577.60AAA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G504460 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT2G18193.11.1e-13553.21P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G18190.14.2e-13253.04P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50940.11.4e-12249.47P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G50930.13.3e-12149.29cytochrome BC1 synthesis [more]
AT5G17760.16.1e-11546.38P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 462..492
NoneNo IPR availableGENE3D6.10.280.40coord: 400..473
e-value: 2.6E-21
score: 77.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 469..492
NoneNo IPR availablePANTHERPTHR23070:SF140BNAA06G25360D PROTEINcoord: 10..472
NoneNo IPR availablePANTHERPTHR23070BCS1 AAA-TYPE ATPASEcoord: 10..472
NoneNo IPR availableCDDcd00009AAAcoord: 244..403
e-value: 6.35339E-9
score: 52.9187
IPR003593AAA+ ATPase domainSMARTSM00382AAA_5coord: 243..405
e-value: 2.5E-7
score: 40.3
IPR025753AAA-type ATPase, N-terminal domainPFAMPF14363AAA_assoccoord: 38..131
e-value: 2.3E-19
score: 69.6
IPR003959ATPase, AAA-type, corePFAMPF00004AAAcoord: 353..402
e-value: 3.6E-5
score: 24.2
coord: 248..317
e-value: 1.1E-9
score: 38.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 200..399
e-value: 1.1E-41
score: 144.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 109..453
IPR003960ATPase, AAA-type, conserved sitePROSITEPS00674AAAcoord: 373..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010565.1MS010565.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity