MS010561 (gene) Bitter gourd (TR) v1

Overview
NameMS010561
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionabscisic acid 8'-hydroxylase 3-like
Locationscaffold35: 573303 .. 576014 (-)
RNA-Seq ExpressionMS010561
SyntenyMS010561
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGTGGAAGGAAGAGCTTGTGTTCTTAGCTCAAAACTATTTTGACACCGCAGTGGTGGTGGTTCTTTCCATAACTATTTTATTGTTGGTTTCAAAAGCTTGGAGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTGCCTTTTGTAGGGGAAACCTTCTCTTTTTTTTCTGCTAATAACAGTACAAAAGGATGTTACCACTTTGTCAGGCTTCGCCGGTTATGGTTAGTGGTTCTTTCTTTCCCCATGGTGATTTCGCATATTCTTTACACAAGTACTATTATCGGTATCTACTTCTAATAATTCTGGATAATTTCTTTAAGATTTTTAATATATATAGCTTATATATGATTTCTTCAACTTCTTAATTAGATAATTCTAAACTTGGCTACAAAACTAGACTTTGATTATCATCACAGTCAACTACAATGAATGTTTACACTTGGCTACTGATGTGAGTTGCATGGCCATTTGGAAAGATACATACATATACATACATAAGCATGTATATACCAGACAGTTTAGAACATGGAATTGATGACTGTGATGAAACTGAACTAGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATCCATGTGTTTGTTCCAAGTGCAGAGGGTGCAAAAGCAATATTTGCCAAGGATTTTGTCCAGTTCAATAAGGGATACGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTGTGCGTTTCCCATGAAAACCACGGAAGGATTAGGCGTCTCCTATCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTCGACGACATGCTCTCCGAAAGGCTAAAGAAACTTGAAAAAGATGGGAAAGGTTTTGTACTACTAGATTTTAGCATGAAGGTGAGTTAAAAAAATTCGTGATCTTTCTCGAACTTTCCTGTTTGACTTGGGCGGCTAACATATCTTATCTTGTGTGATTTGCAGATAACATTTGATTCTATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGCTTGAGAAAGATTGCATTGCCGTCTCTGATGCCATGTTATCTTTCCCATTCATGATCCCAGGAACTAGGTACTACAAAGGCATCAAGGTACGGTCAGCAGCTTTTCAAGATATAGGCACCCAATTTACTAGTACAGTACAATATAATTAATAAGGAGTTTTTGACTTCATTAAACCTTAGTTAGTAACATATATACTAATTACCAACAAACTGAGCAGGCACGAACTCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACAGGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTCCTGATGAAAAGCTAGATGACACAGAGATAATGGATAACCTATTGACACTGATAATTGCAGGCCAAACGACTACAGCTGCTGCTATGATGTGGAGTATCAAGTTCCTGGACGAAAACAGAGAAGCCCAAGAAAGGCTGCGGGTAAGATCCACGGCCTGAAGTTGCTAAAAATAAGCTAACAAACATTACCCATAATCCACGGCTCTTCATATTTTTCATTCCGAGTACTCCTGTGAAGTGACTATTAAATAATATATTCATCCCAAATCTCCAGGAAGAACAATTGTCAATTCTACGAAAGAAGGAATATGGAGAACAGCTTACCCTTGAAGACCTTAACAAAATGTCCTATTGTTTAAAGGTTCTAAGCAACCTACAAATTGTTCTTGTTTTGAAAGAAATAAACAGAGCGATTTAGATTGTAACCAATTCCATGCATAATGCAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGAGTGGCACTAGGGGATTGCAGATTAGAAGGCACGTTTCTCCAGTTATACCCATTCTCTCTCCTTTTGATATTTCTTTCACTAATGGAAGCAAGTGAACACGGTTTTCTACCGTAGAAAAGGAAGTAAACTCCTGTAGGTCTAATTCTGCTGCTAGAAATATACGCATATACATGTACATAAAAGCATCCTATTGGTACATAGATAGAAAGGATGCCATCTGGAAATGATATGCAGGTTTTGAAAAAAAATGATTAGACAGAGGGAACCGGGAACCCCATCCAACTCATTTTGCCCGTGATATGCAGGTTTTGAAATAAAGAAAGGATGGCATGTGAACATCGACGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGTATCTACCTACAATCAATCACTATTTCTTGTTCTTTTTCCTTTTCTTTCGGCTACTAAACATTATAGTTCATGCACCTATGATTCATAACTCCAGGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAGAGTAACAATGATGGTCTTTCTGCACAGAATGACTGGAGGGTACATGTGAGTCTATACATTTCTAAAGCAGCGGGAATTTCATCATGTTTCAAGAGATCATTCAGAATATTGTCTCCTTCTTCTTAACATTGTCCTGATCATAATTATTCTGCTATTGGCTAGGTGGACAGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACCTTGAAGACCTTG

mRNA sequence

ATGTTGTGGAAGGAAGAGCTTGTGTTCTTAGCTCAAAACTATTTTGACACCGCAGTGGTGGTGGTTCTTTCCATAACTATTTTATTGTTGGTTTCAAAAGCTTGGAGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTGCCTTTTGTAGGGGAAACCTTCTCTTTTTTTTCTGCTAATAACAGTACAAAAGGATGTTACCACTTTGTCAGGCTTCGCCGGTTATGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATCCATGTGTTTGTTCCAAGTGCAGAGGGTGCAAAAGCAATATTTGCCAAGGATTTTGTCCAGTTCAATAAGGGATACGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTGTGCGTTTCCCATGAAAACCACGGAAGGATTAGGCGTCTCCTATCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTCGACGACATGCTCTCCGAAAGGCTAAAGAAACTTGAAAAAGATGGGAAAGGTTTTGTACTACTAGATTTTAGCATGAAGATAACATTTGATTCTATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGCTTGAGAAAGATTGCATTGCCGTCTCTGATGCCATGTTATCTTTCCCATTCATGATCCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAACTCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACAGGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTCCTGATGAAAAGCTAGATGACACAGAGATAATGGATAACCTATTGACACTGATAATTGCAGGCCAAACGACTACAGCTGCTGCTATGATGTGGAGTATCAAGTTCCTGGACGAAAACAGAGAAGCCCAAGAAAGGCTGCGGGAAGAACAATTGTCAATTCTACGAAAGAAGGAATATGGAGAACAGCTTACCCTTGAAGACCTTAACAAAATGTCCTATTGTTTAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGAAAAGGATGGCATGTGAACATCGACGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAGAGTAACAATGATGGTCTTTCTGCACAGAATGACTGGAGGGTACATGTGGACAGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACCTTGAAGACCTTG

Coding sequence (CDS)

ATGTTGTGGAAGGAAGAGCTTGTGTTCTTAGCTCAAAACTATTTTGACACCGCAGTGGTGGTGGTTCTTTCCATAACTATTTTATTGTTGGTTTCAAAAGCTTGGAGGAGGTTGAGGAGCAAGAATGTTGGAGGAATTCCTGGTCACTTGGGGTTGCCTTTTGTAGGGGAAACCTTCTCTTTTTTTTCTGCTAATAACAGTACAAAAGGATGTTACCACTTTGTCAGGCTTCGCCGGTTATGGCATGGTAGGTGGTTCAAGACAAGGCTATTTGGAAAGATCCATGTGTTTGTTCCAAGTGCAGAGGGTGCAAAAGCAATATTTGCCAAGGATTTTGTCCAGTTCAATAAGGGATACGTGAAATCCATGGCAGATGCTGTTGGAGAGAAGAGTTTACTGTGCGTTTCCCATGAAAACCACGGAAGGATTAGGCGTCTCCTATCTGATCCTTTCTCCATGACTTCTCTGTCTAAGTTTGTCAAAGAATTCGACGACATGCTCTCCGAAAGGCTAAAGAAACTTGAAAAAGATGGGAAAGGTTTTGTACTACTAGATTTTAGCATGAAGATAACATTTGATTCTATGTGCAATATGCTAATGAGTCTAAGAGATGAATCTACACTCAGACAGCTTGAGAAAGATTGCATTGCCGTCTCTGATGCCATGTTATCTTTCCCATTCATGATCCCAGGAACTAGGTACTACAAAGGCATCAAGGCACGAACTCGACTCATGGAAAGGTTTAGAGAGATAATTTCCAGGCGGCGGAGGGGAGAGGAGTCACAGGAAGACTTCCTGCAGTCAATGTTGCAGAGAGATTCATATCCTCCTGATGAAAAGCTAGATGACACAGAGATAATGGATAACCTATTGACACTGATAATTGCAGGCCAAACGACTACAGCTGCTGCTATGATGTGGAGTATCAAGTTCCTGGACGAAAACAGAGAAGCCCAAGAAAGGCTGCGGGAAGAACAATTGTCAATTCTACGAAAGAAGGAATATGGAGAACAGCTTACCCTTGAAGACCTTAACAAAATGTCCTATTGTTTAAAGGTCGTCAAAGAGACGTTGAGAATGTCCAATGTTTTGCTGTGGTTTCCTCGAAAAGGATGGCATGTGAACATCGACGCAACATGCATTCATTATGACCCAGATTTGTACAAGGACCCCATGCAATTCAACCCTTCCAGATTTGATGAAATTCAAAAGCCATACAGTTTCATACCTTTTGGATCTGGACCCAGGACCTGCCTAGGAATTAACATGGCCAGAGTAACAATGATGGTCTTTCTGCACAGAATGACTGGAGGGTACATGTGGACAGTTGATGATCCAGATCCTTGCCTTGAAAAGAAGGCACATATCCCTAGGTTGAGAAGCGGCTGTCCCATTACCTTGAAGACCTTG

Protein sequence

MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPRKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPITLKTL
Homology
BLAST of MS010561 vs. NCBI nr
Match: XP_022132689.1 (abscisic acid 8'-hydroxylase 1-like [Momordica charantia])

HSP 1 Score: 939.9 bits (2428), Expect = 8.6e-270
Identity = 468/484 (96.69%), Postives = 470/484 (97.11%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60
           MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS
Sbjct: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60

Query: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120
           FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV
Sbjct: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180
           KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG
Sbjct: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180

Query: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTT 300
           RTRLMERFREIISRRRRGEESQEDFLQSMLQRD+YPPDEKLDDTEIMDNLLTLIIAGQTT
Sbjct: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDAYPPDEKLDDTEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSILRKK+YGEQLTLEDLNKMSYCLKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKDYGEQLTLEDLNKMSYCLKVVKETLRM 360

Query: 361 SNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420
           SNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY
Sbjct: 361 SNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420

Query: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 471
           SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 480

BLAST of MS010561 vs. NCBI nr
Match: XP_022950585.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125.1 Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 846.7 bits (2186), Expect = 9.9e-242
Identity = 425/485 (87.63%), Postives = 442/485 (91.13%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLSI IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVSHE+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER REIISRRR+GEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. NCBI nr
Match: XP_022978466.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 846.3 bits (2185), Expect = 1.3e-241
Identity = 424/485 (87.42%), Postives = 442/485 (91.13%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLS+ IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVSHE+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER  EIISRRRRGEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. NCBI nr
Match: XP_023543351.1 (abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 845.9 bits (2184), Expect = 1.7e-241
Identity = 425/485 (87.63%), Postives = 441/485 (90.93%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLSI IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVSHE+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AVSDAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVSDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER REIISRRR GEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRSGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAM WS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMTWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. NCBI nr
Match: KAG6603947.1 (Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 842.4 bits (2175), Expect = 1.9e-240
Identity = 424/485 (87.42%), Postives = 441/485 (90.93%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLSI IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVS E+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSLEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER REIISRRR+GEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. ExPASy Swiss-Prot
Match: Q949P1 (Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.9e-49
Identity = 127/424 (29.95%), Postives = 214/424 (50.47%), Query Frame = 0

Query: 36  RRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIH 95
           RR  S  +   PG +G P+VGETF  +S + +      F + ++  +G  FKT + G   
Sbjct: 27  RRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPC 86

Query: 96  VFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTS 155
           V + S E AK +       F   +  S    +G++++     + H ++R+L+   F   S
Sbjct: 87  VMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPES 146

Query: 156 LSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSL-RDESTLRQLEKD 215
           +   V + + +  + L+  E    G ++  +    T+     +L    +DE   R+  K 
Sbjct: 147 IRNMVPDIESIAQDSLRSWE----GTMINTYQEMKTYTFNVALLSIFGKDEVLYREDLKR 206

Query: 216 CIAVSD-AMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRRRGEESQEDFLQSMLQRD 275
           C  + +    S P  +PGT ++K +KAR  L +    I+S RR+   S  D L S +   
Sbjct: 207 CYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM--- 266

Query: 276 SYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKK 335
                E+L D +I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + K
Sbjct: 267 --GDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDK 326

Query: 336 EYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------KGWHVNIDAT 395
           E GE LT  D  KM    +V++ETLR++++L +  R              KGW V     
Sbjct: 327 EEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 386

Query: 396 CIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGY 444
            IH+  D++ +P +F+PSRF+   KP +F+PFG+G  +C G  +A++ M + +H +T  Y
Sbjct: 387 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 436

BLAST of MS010561 vs. ExPASy Swiss-Prot
Match: Q9FH76 (Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 7.0e-49
Identity = 135/469 (28.78%), Postives = 228/469 (48.61%), Query Frame = 0

Query: 15  FDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHF 74
           F T     L + +L  ++   RR  S  +   PG +G P+VGETF  +S + +      F
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV-----F 66

Query: 75  VRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLC 134
              ++  +G  FKT + G   V + S E AK +       F   +  S    +G++++  
Sbjct: 67  FAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 126

Query: 135 VSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDS 194
              + H ++R+L+   F   ++   V   + +  E L     DG            TF+ 
Sbjct: 127 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNV 186

Query: 195 MCNMLMSLRDESTLRQLEKDCIAVSD-AMLSFPFMIPGTRYYKGIKARTRLMERFREIIS 254
               ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L +    I+S
Sbjct: 187 ALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILS 246

Query: 255 RRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLD 314
           +RR+   S  D L S ++  +      L D +I DN++ +I A + TTA+ + W +K+L 
Sbjct: 247 KRRQNPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLA 306

Query: 315 ENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR---- 374
           +N    E + EEQ++I + K+ GE LT ED  KM    +V++ETLR + +L +  R    
Sbjct: 307 DNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 366

Query: 375 ----------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCL 434
                     KGW V      IH++ D++ DP +F+PSRF+   KP +F+PFGSG  +C 
Sbjct: 367 DVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCP 426

Query: 435 GINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPITLK 469
           G  +A++ + V +H +T  Y W++  P   ++        ++G PI L+
Sbjct: 427 GNELAKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

BLAST of MS010561 vs. ExPASy Swiss-Prot
Match: O81077 (Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 1.9e-46
Identity = 126/456 (27.63%), Postives = 221/456 (48.46%), Query Frame = 0

Query: 9   FLAQNYFDTAVVVVLSI--TILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANN 68
           F +  Y D   ++ L+I   +++L+ K W   + + +   PG +GLP++GET   ++ N 
Sbjct: 11  FFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENP 70

Query: 69  STKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADA 128
           ++     F   R+  +G  FKT + G   V + S E A+ +       F   Y  S    
Sbjct: 71  NS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERM 130

Query: 129 VGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDF 188
           +G ++L       H  ++RL+   F  ++L   V   + ++ + L       K    L++
Sbjct: 131 IGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEY 190

Query: 189 SMKITFDSMCNMLMSLRDESTLRQLEKDCI-AVSDAMLSFPFMIPGTRYYKGIKARTRLM 248
             +  FD         ++E T   + K     +     S P  +PGT ++K +KAR  L 
Sbjct: 191 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 250

Query: 249 ERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEK---LDDTEIMDNLLTLIIAGQTTTA 308
           E  R++I +RR     +   L  +L       D+K   L D++I DN++ +I A   TTA
Sbjct: 251 EELRKVIEKRRENGREEGGLLGVLLG----AKDQKRNGLSDSQIADNIIGVIFAATDTTA 310

Query: 309 AAMMWSIKFLDENREAQERLREEQLSILRK-KEYGEQLTLEDLNKMSYCLKVVKETLRMS 368
           + + W +K+L ++    + +  EQ SI +K K+   +++ ED  KM    +V++ETLR +
Sbjct: 311 SVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAA 370

Query: 369 NVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYS 428
           +VL +  R              KGW V      IH+  + + DP +F+PSRF+   KPY+
Sbjct: 371 SVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYT 430

Query: 429 FIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTV 444
           ++PFG+G  +C G  +A++ M++ LH +T  + W V
Sbjct: 431 YMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEV 456

BLAST of MS010561 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 9.5e-46
Identity = 135/436 (30.96%), Postives = 203/436 (46.56%), Query Frame = 0

Query: 36  RRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIH 95
           RR R + +G  PG LGLP +GETF    A   T+    F+  R   +G  F T LFG+  
Sbjct: 22  RRTRYRRMGLPPGSLGLPLIGETFQLIGA-YKTENPEPFIDERVARYGSVFMTHLFGEPT 81

Query: 96  VFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTS 155
           +F    E  + +   +   F   Y  S+ + +G+ SLL +    H R+  L    F+ +S
Sbjct: 82  IFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMS-FANSS 141

Query: 156 LSKFVKEFDDMLSERLKKLEKD--GKGFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEK 215
           +   +K+   +  +RL +   D      +L++ + KITF+     LMS         L K
Sbjct: 142 I---IKDHLMLDIDRLVRFNLDSWSSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRK 201

Query: 216 DCIAVSDAMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRR----RGEESQEDFLQSM 275
           + + V +   S P  +  T Y K I+AR ++ E    ++ +RR     G E ++D L ++
Sbjct: 202 EYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAAL 261

Query: 276 LQRDSYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSI 335
           L       D+   D EI+D L+ L++AG  TT+  M  ++KFL E   A  +L+EE   I
Sbjct: 262 L-----AADDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKI 321

Query: 336 LRKKEYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------KGWHVN 395
              K     L   D   M +   VV ETLR++N++    R              KGW V 
Sbjct: 322 RAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVF 381

Query: 396 IDATCIHYDPDLYKDPMQFNPSRFDEIQKPYS----FIPFGSGPRTCLGINMARVTMMVF 448
                +H DP+ +KD   FNP R+            F PFG GPR C G  +ARV + VF
Sbjct: 382 SSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVF 441

BLAST of MS010561 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 2.1e-45
Identity = 140/460 (30.43%), Postives = 208/460 (45.22%), Query Frame = 0

Query: 32  SKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLF 91
           +KAWR       G  PG  G P +GET  F            F   R   +G  F T L 
Sbjct: 45  TKAWR-------GIPPGTFGWPLIGETLEFLGCQRKGNP-RDFFDSRTQKYGNVFTTSLV 104

Query: 92  GKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVG---EKSLLCVSHENHGRIRRLLS 151
           G   V   S EG + +F+ +    NK  V S   +VG     SL+    ++  R+RR+L 
Sbjct: 105 GHPTVVFCSPEGNRFLFSNE----NKLVVNSWPSSVGNLFRSSLITTVGDDAKRLRRILM 164

Query: 152 DPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSLRDESTL 211
                 +L +FV   D M    L +        + L    + TF   C++  S+  +  L
Sbjct: 165 TFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINTKDDL 224

Query: 212 RQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRR----RGEESQED 271
            +L    +     ++  P  +PGTRY K   A   + ++   II+ R+     G  S E 
Sbjct: 225 DRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNASPEQ 284

Query: 272 FLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLRE 331
            L S L  +     E L D EI DN+L L+ AG  T+++ +   +KFL EN    E +  
Sbjct: 285 DLLSFLLSNVDEQGESLTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCYEEVLR 344

Query: 332 EQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------K 391
           EQL+I   KE G+ L  EDL +M Y  +V +E LR+   +    R              K
Sbjct: 345 EQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDGFTIPK 404

Query: 392 GWHVNIDATCIHYDPDLYKDPMQFNPSRFD-EIQKPYSFIPFGSGPRTCLGINMARVTMM 451
           GW ++      H   + + +P +F+PSRF+ E   PY+F+PFG GPR C G   AR+ ++
Sbjct: 405 GWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGPPPYTFVPFGGGPRMCPGNEFARMEIL 464

Query: 452 VFLHRMTGGYMWTVDDPDPCLEKKA--HIPRLRSGCPITL 468
           +FLH +   + W + +P   LEK     +P   +G PI L
Sbjct: 465 IFLHNIVKNFNWNLVNP---LEKVIVDPMPAPVNGLPIKL 489

BLAST of MS010561 vs. ExPASy TrEMBL
Match: A0A6J1BTT9 (abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC111005488 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 4.2e-270
Identity = 468/484 (96.69%), Postives = 470/484 (97.11%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60
           MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS
Sbjct: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60

Query: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120
           FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV
Sbjct: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180
           KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG
Sbjct: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180

Query: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTT 300
           RTRLMERFREIISRRRRGEESQEDFLQSMLQRD+YPPDEKLDDTEIMDNLLTLIIAGQTT
Sbjct: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDAYPPDEKLDDTEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSILRKK+YGEQLTLEDLNKMSYCLKVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKDYGEQLTLEDLNKMSYCLKVVKETLRM 360

Query: 361 SNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420
           SNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY
Sbjct: 361 SNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420

Query: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 471
           SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 480

BLAST of MS010561 vs. ExPASy TrEMBL
Match: A0A6J1GG59 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453639 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.8e-242
Identity = 425/485 (87.63%), Postives = 442/485 (91.13%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLSI IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSIGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVSHE+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AVSDAMLS PFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCSAVSDAMLSLPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER REIISRRR+GEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLREIISRRRKGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. ExPASy TrEMBL
Match: A0A6J1IMR3 (abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478438 PE=3 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 6.3e-242
Identity = 424/485 (87.42%), Postives = 442/485 (91.13%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFD-TAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETF 60
           MLWKEELV LAQNYFD T V  VLS+ IL+LVSK   R RSKNVGGIPGHLGLPFVGETF
Sbjct: 1   MLWKEELVLLAQNYFDTTTVAAVLSVGILVLVSKVCWRQRSKNVGGIPGHLGLPFVGETF 60

Query: 61  SFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGY 120
           SF SANNST+GCY FVRLRRLWHGRWF+TRLFGKIHVFVPSAEGAKAI A DFVQFNKGY
Sbjct: 61  SFLSANNSTRGCYQFVRLRRLWHGRWFRTRLFGKIHVFVPSAEGAKAILANDFVQFNKGY 120

Query: 121 VKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGK 180
           VKSM DAVGEKSLLCVSHE+H RIRRLL+DPFSMTSLSKFVK+FDDMLS+RL+KL KDGK
Sbjct: 121 VKSMGDAVGEKSLLCVSHEDHRRIRRLLADPFSMTSLSKFVKQFDDMLSKRLQKLVKDGK 180

Query: 181 GFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIK 240
            FVLLDFSMKITFDSMCNMLMSLRDESTLRQ+EKDC AV+DAMLSFPFMIPGTRYYKGIK
Sbjct: 181 SFVLLDFSMKITFDSMCNMLMSLRDESTLRQIEKDCTAVTDAMLSFPFMIPGTRYYKGIK 240

Query: 241 ARTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQT 300
           AR RLMER  EIISRRRRGEES EDFLQSMLQRDSYPPD+KLDD+EIMDNLLTLIIA QT
Sbjct: 241 ARNRLMERLGEIISRRRRGEESPEDFLQSMLQRDSYPPDQKLDDSEIMDNLLTLIIASQT 300

Query: 301 TTAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLR 360
           TTAAAMMWS+KFLDENREAQERLREEQLSILRKKE GE LTLEDLNKMS+  KVVKETLR
Sbjct: 301 TTAAAMMWSVKFLDENREAQERLREEQLSILRKKEDGELLTLEDLNKMSFGSKVVKETLR 360

Query: 361 MSNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420
           MSNVLLWFPR              KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP
Sbjct: 361 MSNVLLWFPRVALGDCRLEGFEIKKGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKP 420

Query: 421 YSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPI 471
           YSFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKKAHIPRLRSGCPI
Sbjct: 421 YSFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKAHIPRLRSGCPI 480

BLAST of MS010561 vs. ExPASy TrEMBL
Match: A0A5A7SHU1 (Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001790 PE=3 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.5e-240
Identity = 420/484 (86.78%), Postives = 439/484 (90.70%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60
           M+  E L F+AQ YF+  V  +LSI IL+LVSK W RLRSKN G IPGHLGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120
           F SANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF  DFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180
           KSM DAVGEKSLLCVSHENH RIRRLLSDPFSMTSLSKFVKEFDDMLS+RLKKLEKDGK 
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQ+EKDC AVSDAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTT 300
           R RLME+FREIISRRRRGEES EDFL+SMLQRDSYP +EKLDD+EIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSIL+KKE GE L LEDLNKMSY  KVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 361 SNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420
           SNVLLWFPR              KGWHVNIDATCIHYDPD+YKDPM+FNPSRFDEIQKPY
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEFNPSRFDEIQKPY 420

Query: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 471
           SFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

BLAST of MS010561 vs. ExPASy TrEMBL
Match: A0A1S3B1V7 (abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo OX=3656 GN=LOC103485076 PE=3 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.4e-240
Identity = 419/484 (86.57%), Postives = 439/484 (90.70%), Query Frame = 0

Query: 1   MLWKEELVFLAQNYFDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFS 60
           M+  E L F+AQ YF+  V  +LSI IL+LVSK W RLRSKN G IPGHLGLPFVGETFS
Sbjct: 1   MVCTEGLAFIAQIYFNPTVAALLSIAILVLVSKVWWRLRSKNDGEIPGHLGLPFVGETFS 60

Query: 61  FFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYV 120
           F SANNST+GCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIF  DFVQFNKGYV
Sbjct: 61  FLSANNSTRGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFTNDFVQFNKGYV 120

Query: 121 KSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKG 180
           KSM DAVGEKSLLCVSHENH RIRRLLSDPFSMTSLSKFVKEFDDMLS+RLKKLEKDGK 
Sbjct: 121 KSMGDAVGEKSLLCVSHENHRRIRRLLSDPFSMTSLSKFVKEFDDMLSKRLKKLEKDGKS 180

Query: 181 FVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIAVSDAMLSFPFMIPGTRYYKGIKA 240
           FVLLDFSMKITFDSMCNMLMSL+DESTLRQ+EKDC AVSDAMLSFPFMIPGTRYYKGIKA
Sbjct: 181 FVLLDFSMKITFDSMCNMLMSLKDESTLRQIEKDCKAVSDAMLSFPFMIPGTRYYKGIKA 240

Query: 241 RTRLMERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTT 300
           R RLME+FREIISRRRRGEES EDFL+SMLQRDSYP +EKLDD+EIMDNLLTLIIAGQTT
Sbjct: 241 RNRLMEKFREIISRRRRGEESPEDFLESMLQRDSYPSNEKLDDSEIMDNLLTLIIAGQTT 300

Query: 301 TAAAMMWSIKFLDENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRM 360
           TAAAMMWSIKFLDENREAQERLREEQLSIL+KKE GE L LEDLNKMSY  KVVKETLRM
Sbjct: 301 TAAAMMWSIKFLDENREAQERLREEQLSILQKKEDGELLCLEDLNKMSYGSKVVKETLRM 360

Query: 361 SNVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPY 420
           SNVLLWFPR              KGWHVNIDATCIHYDPD+YKDPM++NPSRFDEIQKPY
Sbjct: 361 SNVLLWFPRVALGDCRLEDFEIKKGWHVNIDATCIHYDPDVYKDPMEYNPSRFDEIQKPY 420

Query: 421 SFIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPIT 471
           SFIPFGSGPRTCLGINMA++TM+VFLHRMTGGY WTVDDPDPCLEKK HIPRLRSGCPIT
Sbjct: 421 SFIPFGSGPRTCLGINMAKLTMLVFLHRMTGGYRWTVDDPDPCLEKKTHIPRLRSGCPIT 480

BLAST of MS010561 vs. TAIR 10
Match: AT1G19630.1 (cytochrome P450, family 722, subfamily A, polypeptide 1 )

HSP 1 Score: 359.8 bits (922), Expect = 3.4e-99
Identity = 198/446 (44.39%), Postives = 272/446 (60.99%), Query Frame = 0

Query: 39  RSKNVGGI-PGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIHVF 98
           R K+  G+ PG  G P +GET  F  + NS KG Y FVR RR+ +G  F+T LFG+ HVF
Sbjct: 28  RKKSTAGVPPGSDGFPVIGETLQFMLSVNSGKGFYEFVRSRRIRYGSCFRTSLFGETHVF 87

Query: 99  VPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTSLS 158
           + + E A+ +   D   F K Y+KS+ + VG++SLLC    +H  +R  L + FS  S +
Sbjct: 88  LSTTESARLVLNNDSGMFTKRYIKSIGELVGDRSLLCAPQHHHKILRSRLINLFSKRSTA 147

Query: 159 KFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSLRDESTLRQLEKDCIA 218
             V+ FD+++ + L   E  G   +L D  ++ITF +MC ML+SL  E  L  +++D   
Sbjct: 148 LMVRHFDELVVDALGGWEHRGTVVLLTDL-LQITFKAMCKMLVSLEKEEELGSMQRDVGF 207

Query: 219 VSDAMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRRRGEES----QEDFLQSMLQRD 278
           V +AML+FP  +P TR++KGI AR R+ME   +II  RR    S     EDFLQ +L  D
Sbjct: 208 VCEAMLAFPLNLPWTRFHKGIMARGRVMEMLEKIIRERRNEINSHNNHHEDFLQQLLAVD 267

Query: 279 SYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKK 338
           +  P  +L D EI DN+LT+IIAGQ TTA+A+ W +K+L EN++  + L EEQ  I +K 
Sbjct: 268 NDTP--QLTDAEIKDNILTMIIAGQDTTASALTWMVKYLGENQKVLDILIEEQSQITKKA 327

Query: 339 EYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------KGWHVNIDAT 398
                L LEDL++M Y  K+VKE+LRM++V+ WFPR              KGW++NIDA 
Sbjct: 328 SNKPFLELEDLSEMPYASKMVKESLRMASVVPWFPRLVLQDCEMEGYKIKKGWNINIDAR 387

Query: 399 CIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGY 458
            IH DP +Y +P +FNP RF+E  K  SF+ FG G RTCLG+ +A+  M+VFLHR    Y
Sbjct: 388 SIHLDPTVYSEPHKFNPLRFEEEAKANSFLAFGMGGRTCLGLALAKAMMLVFLHRFITTY 447

Query: 459 MWTVDDPDPCLEKKAHIPRLRSGCPI 466
            W V D DP +EK     RL+SG PI
Sbjct: 448 RWEVVDEDPSIEKWTLFARLKSGYPI 470

BLAST of MS010561 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-50
Identity = 127/424 (29.95%), Postives = 214/424 (50.47%), Query Frame = 0

Query: 36  RRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIH 95
           RR  S  +   PG +G P+VGETF  +S + +      F + ++  +G  FKT + G   
Sbjct: 27  RRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPC 86

Query: 96  VFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTS 155
           V + S E AK +       F   +  S    +G++++     + H ++R+L+   F   S
Sbjct: 87  VMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPES 146

Query: 156 LSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSL-RDESTLRQLEKD 215
           +   V + + +  + L+  E    G ++  +    T+     +L    +DE   R+  K 
Sbjct: 147 IRNMVPDIESIAQDSLRSWE----GTMINTYQEMKTYTFNVALLSIFGKDEVLYREDLKR 206

Query: 216 CIAVSD-AMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRRRGEESQEDFLQSMLQRD 275
           C  + +    S P  +PGT ++K +KAR  L +    I+S RR+   S  D L S +   
Sbjct: 207 CYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM--- 266

Query: 276 SYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKK 335
                E+L D +I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + K
Sbjct: 267 --GDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDK 326

Query: 336 EYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------KGWHVNIDAT 395
           E GE LT  D  KM    +V++ETLR++++L +  R              KGW V     
Sbjct: 327 EEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 386

Query: 396 CIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGY 444
            IH+  D++ +P +F+PSRF+   KP +F+PFG+G  +C G  +A++ M + +H +T  Y
Sbjct: 387 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 436

BLAST of MS010561 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 196.4 bits (498), Expect = 5.0e-50
Identity = 135/469 (28.78%), Postives = 228/469 (48.61%), Query Frame = 0

Query: 15  FDTAVVVVLSITILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHF 74
           F T     L + +L  ++   RR  S  +   PG +G P+VGETF  +S + +      F
Sbjct: 7   FLTLSAAALFLCLLRFIAGV-RRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNV-----F 66

Query: 75  VRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLC 134
              ++  +G  FKT + G   V + S E AK +       F   +  S    +G++++  
Sbjct: 67  FAAKQRRYGSVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFF 126

Query: 135 VSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDS 194
              + H ++R+L+   F   ++   V   + +  E L     DG            TF+ 
Sbjct: 127 HQGDYHSKLRKLVLRAFMPDAIRNMVPHIESIAQESLNSW--DGTQLNTYQEMKTYTFNV 186

Query: 195 MCNMLMSLRDESTLRQLEKDCIAVSD-AMLSFPFMIPGTRYYKGIKARTRLMERFREIIS 254
               ++  +DE   R+  K C  + +    S P  +PGT ++K +KAR  L +    I+S
Sbjct: 187 ALISILG-KDEVYYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILS 246

Query: 255 RRRRGEESQEDFLQSMLQRDSYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLD 314
           +RR+   S  D L S ++  +      L D +I DN++ +I A + TTA+ + W +K+L 
Sbjct: 247 KRRQNPSSHTDLLGSFMEDKA-----GLTDEQIADNIIGVIFAARDTTASVLTWILKYLA 306

Query: 315 ENREAQERLREEQLSILRKKEYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR---- 374
           +N    E + EEQ++I + K+ GE LT ED  KM    +V++ETLR + +L +  R    
Sbjct: 307 DNPTVLEAVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVE 366

Query: 375 ----------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCL 434
                     KGW V      IH++ D++ DP +F+PSRF+   KP +F+PFGSG  +C 
Sbjct: 367 DVEYEGYLIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFEVAPKPNTFMPFGSGIHSCP 426

Query: 435 GINMARVTMMVFLHRMTGGYMWTVDDPDPCLEKKAHIPRLRSGCPITLK 469
           G  +A++ + V +H +T  Y W++  P   ++        ++G PI L+
Sbjct: 427 GNELAKLEISVLIHHLTTKYRWSIVGPSDGIQ-YGPFALPQNGLPIALE 460

BLAST of MS010561 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 193.0 bits (489), Expect = 5.5e-49
Identity = 126/420 (30.00%), Postives = 211/420 (50.24%), Query Frame = 0

Query: 36  RRLRSKNVGGIPGHLGLPFVGETFSFFSANNSTKGCYHFVRLRRLWHGRWFKTRLFGKIH 95
           RR  S  +   PG +G P+VGETF  +S + +      F + ++  +G  FKT + G   
Sbjct: 27  RRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNV-----FFQSKQKRYGSVFKTHVLGCPC 86

Query: 96  VFVPSAEGAKAIFAKDFVQFNKGYVKSMADAVGEKSLLCVSHENHGRIRRLLSDPFSMTS 155
           V + S E AK +       F   +  S    +G++++     + H ++R+L+   F   S
Sbjct: 87  VMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKLRKLVLRAFMPES 146

Query: 156 LSKFVKEFDDMLSERLKKLEKDGKGFVLLDFSMKITFDSMCNMLMSL-RDESTLRQLEKD 215
           +   V + + +  + L+  E    G ++  +    T+     +L    +DE   R+  K 
Sbjct: 147 IRNMVPDIESIAQDSLRSWE----GTMINTYQEMKTYTFNVALLSIFGKDEVLYREDLKR 206

Query: 216 CIAVSD-AMLSFPFMIPGTRYYKGIKARTRLMERFREIISRRRRGEESQEDFLQSMLQRD 275
           C  + +    S P  +PGT ++K +KAR  L +    I+S RR+   S  D L S +   
Sbjct: 207 CYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERRQNGSSHNDLLGSFM--- 266

Query: 276 SYPPDEKLDDTEIMDNLLTLIIAGQTTTAAAMMWSIKFLDENREAQERLREEQLSILRKK 335
                E+L D +I DN++ +I A + TTA+ M W +K+L EN    E + EEQ++I + K
Sbjct: 267 --GDKEELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNVLEAVTEEQMAIRKDK 326

Query: 336 EYGEQLTLEDLNKMSYCLKVVKETLRMSNVLLWFPR--------------KGWHVNIDAT 395
           E GE LT  D  KM    +V++ETLR++++L +  R              KGW V     
Sbjct: 327 EEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYEGYLIPKGWKVLPLFR 386

Query: 396 CIHYDPDLYKDPMQFNPSRFDEIQKPYSFIPFGSGPRTCLGINMARVTMMVFLHRMTGGY 440
            IH+  D++ +P +F+PSRF+   KP +F+PFG+G  +C G  +A++ M + +H +T  Y
Sbjct: 387 NIHHSADIFSNPGKFDPSRFEVAPKPNTFMPFGNGTHSCPGNELAKLEMSIMIHHLTTKY 432

BLAST of MS010561 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 188.3 bits (477), Expect = 1.4e-47
Identity = 126/456 (27.63%), Postives = 221/456 (48.46%), Query Frame = 0

Query: 9   FLAQNYFDTAVVVVLSI--TILLLVSKAWRRLRSKNVGGIPGHLGLPFVGETFSFFSANN 68
           F +  Y D   ++ L+I   +++L+ K W   + + +   PG +GLP++GET   ++ N 
Sbjct: 11  FFSSLYADEPALITLTIVVVVVVLLFKWWLHWKEQRLRLPPGSMGLPYIGETLRLYTENP 70

Query: 69  STKGCYHFVRLRRLWHGRWFKTRLFGKIHVFVPSAEGAKAIFAKDFVQFNKGYVKSMADA 128
           ++     F   R+  +G  FKT + G   V + S E A+ +       F   Y  S    
Sbjct: 71  NS-----FFATRQNKYGDIFKTHILGCPCVMISSPEAARMVLVSKAHLFKPTYPPSKERM 130

Query: 129 VGEKSLLCVSHENHGRIRRLLSDPFSMTSLSKFVKEFDDMLSERLKKLEKDGKGFVLLDF 188
           +G ++L       H  ++RL+   F  ++L   V   + ++ + L       K    L++
Sbjct: 131 IGPEALFFHQGPYHSTLKRLVQSSFMPSALRPTVSHIELLVLQTLSSWTSQ-KSINTLEY 190

Query: 189 SMKITFDSMCNMLMSLRDESTLRQLEKDCI-AVSDAMLSFPFMIPGTRYYKGIKARTRLM 248
             +  FD         ++E T   + K     +     S P  +PGT ++K +KAR  L 
Sbjct: 191 MKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLERGYNSMPLDLPGTLFHKSMKARIELS 250

Query: 249 ERFREIISRRRRGEESQEDFLQSMLQRDSYPPDEK---LDDTEIMDNLLTLIIAGQTTTA 308
           E  R++I +RR     +   L  +L       D+K   L D++I DN++ +I A   TTA
Sbjct: 251 EELRKVIEKRRENGREEGGLLGVLLG----AKDQKRNGLSDSQIADNIIGVIFAATDTTA 310

Query: 309 AAMMWSIKFLDENREAQERLREEQLSILRK-KEYGEQLTLEDLNKMSYCLKVVKETLRMS 368
           + + W +K+L ++    + +  EQ SI +K K+   +++ ED  KM    +V++ETLR +
Sbjct: 311 SVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKENRRISWEDTRKMPLTTRVIQETLRAA 370

Query: 369 NVLLWFPR--------------KGWHVNIDATCIHYDPDLYKDPMQFNPSRFDEIQKPYS 428
           +VL +  R              KGW V      IH+  + + DP +F+PSRF+   KPY+
Sbjct: 371 SVLSFTFREAVQDVEYDGYLIPKGWKVLPLFRRIHHSSEFFPDPEKFDPSRFEVAPKPYT 430

Query: 429 FIPFGSGPRTCLGINMARVTMMVFLHRMTGGYMWTV 444
           ++PFG+G  +C G  +A++ M++ LH +T  + W V
Sbjct: 431 YMPFGNGVHSCPGSELAKLEMLILLHHLTTSFRWEV 456

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022132689.18.6e-27096.69abscisic acid 8'-hydroxylase 1-like [Momordica charantia][more]
XP_022950585.19.9e-24287.63abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita moschata] >KAG7034125.... [more]
XP_022978466.11.3e-24187.42abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita maxima][more]
XP_023543351.11.7e-24187.63abscisic acid 8'-hydroxylase 3-like isoform X2 [Cucurbita pepo subsp. pepo][more]
KAG6603947.11.9e-24087.42Abscisic acid 8'-hydroxylase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q949P11.9e-4929.95Abscisic acid 8'-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=CYP707A1 PE=1 ... [more]
Q9FH767.0e-4928.78Abscisic acid 8'-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=CYP707A3 PE=1 ... [more]
O810771.9e-4627.63Abscisic acid 8'-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=CYP707A2 PE=2 ... [more]
Q425699.5e-4630.96Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Q50EK12.1e-4530.43Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1BTT94.2e-27096.69abscisic acid 8'-hydroxylase 1-like OS=Momordica charantia OX=3673 GN=LOC1110054... [more]
A0A6J1GG594.8e-24287.63abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1IMR36.3e-24287.42abscisic acid 8'-hydroxylase 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5A7SHU11.5e-24086.78Abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A1S3B1V73.4e-24086.57abscisic acid 8'-hydroxylase 1-like OS=Cucumis melo OX=3656 GN=LOC103485076 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G19630.13.4e-9944.39cytochrome P450, family 722, subfamily A, polypeptide 1 [more]
AT4G19230.11.3e-5029.95cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT5G45340.15.0e-5028.78cytochrome P450, family 707, subfamily A, polypeptide 3 [more]
AT4G19230.25.5e-4930.00cytochrome P450, family 707, subfamily A, polypeptide 1 [more]
AT2G29090.11.4e-4727.63cytochrome P450, family 707, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001128Cytochrome P450PRINTSPR00385P450coord: 409..418
score: 58.94
coord: 352..363
score: 36.96
coord: 418..429
score: 33.6
coord: 296..313
score: 30.91
IPR001128Cytochrome P450PFAMPF00067p450coord: 48..446
e-value: 2.5E-54
score: 184.8
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 408..418
score: 53.98
coord: 305..331
score: 20.48
coord: 418..441
score: 23.38
coord: 285..302
score: 28.47
coord: 377..401
score: 30.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 36..470
e-value: 7.8E-96
score: 323.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 37..468
NoneNo IPR availablePIRSRPIRSR000047-1PIRSR000047-1coord: 21..432
e-value: 4.1E-10
score: 37.0
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 18..468
NoneNo IPR availablePANTHERPTHR24286:SF235CYTOCHROME P450 FAMILY PROTEINcoord: 18..468
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 411..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010561.1MS010561.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen