MS010530 (gene) Bitter gourd (TR) v1

BLAST of MS010530 vs. NCBI nr
Match: XP_022133230.1 (protein N-lysine methyltransferase METTL21A-like [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 1.9e-147
Identity = 273/279 (97.85%), Postives = 274/279 (98.21%), Query Frame = 0

Query: 1   MSAEHD---GGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL 60
           MSAEHD   GGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL
Sbjct: 20  MSAEHDGGGGGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL 79

Query: 61  PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLV 120
           PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALR+GSDRRTLEVLEIGSGTGLV
Sbjct: 80  PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALRDGSDRRTLEVLEIGSGTGLV 139

Query: 121 GIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGR 180
           GIAAAATLGA VTVTDLSHVIPNLQFNVEANAEVLAANGG VQVAPLHWGEAIDTELIGR
Sbjct: 140 GIAAAATLGAIVTVTDLSHVIPNLQFNVEANAEVLAANGGRVQVAPLHWGEAIDTELIGR 199

Query: 181 EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF 240
           EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF
Sbjct: 200 EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF 259

Query: 241 EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG
Sbjct: 260 EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 298

BLAST of MS010530 vs. NCBI nr
Match: XP_022950392.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita moschata])

HSP 1 Score: 459.9 bits (1182), Expect = 1.5e-125
Identity = 233/278 (83.81%), Postives = 256/278 (92.09%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  DDI PISVIL  E   I+KLSL ++GV SELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDADDISPISVILPAEAAGINKLSLPNDGVVSELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTP LTA R+GS+RRTL+VLEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTPILTASRDGSERRTLKVLEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAID ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIDPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPL+QTLR FLLGGGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDPLIQTLRGFLLGGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKPAIGSSNQG 277

BLAST of MS010530 vs. NCBI nr
Match: XP_022978541.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita maxima])

HSP 1 Score: 459.5 bits (1181), Expect = 2.0e-125
Identity = 233/278 (83.81%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  DDI PISVIL  EE  I+KLSL ++GVASELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDDDDISPISVILPAEEAGINKLSLPNDGVASELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTPTLTA R+GS+RRTL++LEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTPTLTASRDGSERRTLKILEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAI  ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIYPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPL+QTL  FLLGGGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDPLIQTLSGFLLGGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKPAIGSSNQG 277

BLAST of MS010530 vs. NCBI nr
Match: XP_023543861.1 (protein N-lysine methyltransferase METTL21A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 458.8 bits (1179), Expect = 3.4e-125
Identity = 232/278 (83.45%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  D+I PISVIL  EE  I+KLS  ++GVASELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDDDEISPISVILPAEEAGINKLSFPNDGVASELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLT TLTA R+GS+RRTL++LEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTATLTASRDGSERRTLKILEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAID ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIDPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPL+QTLR FLLGGGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDPLIQTLRGFLLGGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKLAIGSSNQG 277

BLAST of MS010530 vs. NCBI nr
Match: KAG7034148.1 (Protein-lysine methyltransferase METTL21C, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 453.0 bits (1164), Expect = 1.9e-123
Identity = 231/278 (83.09%), Postives = 254/278 (91.37%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  DDI PISVIL  EE  I+KLSL ++GV SELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDADDISPISVILPAEEAGINKLSLPNDGVVSELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTP LTA R+GS+RRTL+VLEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTPILTASRDGSERRTLKVLEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAI  ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIGPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYD L+QTLR FLL GGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDLLIQTLRGFLLDGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKPAIGSSNQG 277

BLAST of MS010530 vs. ExPASy Swiss-Prot
Match: Q5VZV1 (Protein-lysine methyltransferase METTL21C OS=Homo sapiens OX=9606 GN=METTL21C PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.0e-15
Identity = 69/212 (32.55%), Postives = 98/212 (46.23%), Query Frame = 0

Query: 37  ASELQHHFIRSINSTLAIRQLPSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTAL 96
           AS  Q H+  +    +    + S G    +WP A  L + L+E                 
Sbjct: 64  ASYTQEHYRFAGKEIVIQESIESYGA--VVWPGAMALCQYLEEH---------------- 123

Query: 97  REGSDRRTLEVLEIGSGTGLVGIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANG 156
            E  + +  ++LEIG+G GLV I  A+ LGA+VT TDL  V+ NLQ+N+  N   L    
Sbjct: 124 AEELNFQDAKILEIGAGPGLVSI-VASILGAQVTATDLPDVLGNLQYNLLKN--TLQCTA 183

Query: 157 GCVQVAPLHWGEAIDTELIGREF--ELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMV 216
              +V  L WGE +D       F  + VLASDVVYH +  D LL T+         P  V
Sbjct: 184 HLPEVKELVWGEDLDKNFPKSAFYYDYVLASDVVYHHYFLDKLLTTMVYL----SQPGTV 243

Query: 217 FLMAHLRRWKKDSAFFKKARKLFEVEVLHTDP 247
            L A+  R+  D  F  K +++F+  +L   P
Sbjct: 244 LLWANKFRFSTDYEFLDKFKQVFDTTLLAEYP 250

BLAST of MS010530 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.5e-14
Identity = 54/145 (37.24%), Postives = 79/145 (54.48%), Query Frame = 0

Query: 101 DRRTLEVLEIGSGTGLVGIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQ 160
           D +   V+E+G+GTGLVGI  AA LGA VT+TD    +  L  NV  N  +       VQ
Sbjct: 63  DLKGKRVIELGAGTGLVGI-VAALLGANVTITDREPALEFLTANVHEN--IPQGRQKAVQ 122

Query: 161 VAPLHWGEAIDTELIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHL 220
           V+ L WGE +D    G  ++L+L +D+VY +  +  LLQTL     G      V L++  
Sbjct: 123 VSELTWGENLDLYPQG-GYDLILGADIVYLEETFPALLQTLEHLSSG----DTVVLLSCR 182

Query: 221 RRWKKDSAFFKKARKLFEVEVLHTD 246
            R+++D  F  + R+ F V+ +H D
Sbjct: 183 IRYERDERFLTELRQRFSVQEVHYD 199

BLAST of MS010530 vs. ExPASy Swiss-Prot
Match: A6QP81 (Protein-lysine methyltransferase METTL21C OS=Bos taurus OX=9913 GN=METTL21C PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-14
Identity = 63/179 (35.20%), Postives = 88/179 (49.16%), Query Frame = 0

Query: 66  LWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVGIAAAATL 125
           +WP A  L + L+E   H E   L             R  ++LEIG+G GLV I  A+ L
Sbjct: 84  VWPGAMALCQYLEE---HTEELNL-------------RGAKILEIGAGPGLVSI-VASIL 143

Query: 126 GAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE--FELVL 185
           GA+VT TDL  V+ NLQ+N+  N   L       +V  L WGE ++         ++ VL
Sbjct: 144 GAQVTATDLPDVLGNLQYNLLKN--TLNCTTYLPEVKELVWGEGLEQSFPKSTLYYDYVL 203

Query: 186 ASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFEVEVL 243
           ASDVVYH +  D LL T+         P  V L A+  R+  D  F  K +++F+  +L
Sbjct: 204 ASDVVYHHYFLDKLLATMVYLC----QPGTVLLWANKFRFSTDYEFLDKFKQVFDTTLL 239

BLAST of MS010530 vs. ExPASy Swiss-Prot
Match: Q8BLU2 (Protein-lysine methyltransferase METTL21C OS=Mus musculus OX=10090 GN=Mettl21c PE=2 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 3.3e-14
Identity = 61/179 (34.08%), Postives = 88/179 (49.16%), Query Frame = 0

Query: 66  LWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVGIAAAATL 125
           +WP AT L + L++   H E   L             +  ++LEIG+G GLV I  ++ L
Sbjct: 75  VWPGATALCQYLED---HTEELNL-------------QDAKILEIGAGAGLVSI-VSSLL 134

Query: 126 GAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE--FELVL 185
           GA+VT TDL  V+ NLQ+N+  N   L       +V  L WGE ++         ++ VL
Sbjct: 135 GAQVTATDLPDVLGNLQYNILKN--TLECTAHLPEVRELVWGEDLEQSFPKSTCCYDYVL 194

Query: 186 ASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFEVEVL 243
           ASDVVYH +  D LL T+         P  V L A+  R+  D  F  K ++ F+  +L
Sbjct: 195 ASDVVYHHYFLDKLLATMVYL----SQPGTVVLWANKFRFSADYEFLGKFKQAFDTTLL 230

BLAST of MS010530 vs. ExPASy Swiss-Prot
Match: Q9CQL0 (Protein N-lysine methyltransferase METTL21A OS=Mus musculus OX=10090 GN=Mettl21A PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 9.6e-14
Identity = 65/199 (32.66%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 49  NSTLAIRQ-LPSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEV 108
           N T+ IRQ     G++  +W AA  L   L+                      + R    
Sbjct: 28  NHTIQIRQDWRQLGVAAVVWDAAVVLSMYLE------------------MGAVELRGCSA 87

Query: 109 LEIGSGTGLVGIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWG 168
           +E+G+GTGLVGI  AA LGA+VT+TD    +  L+ NVEAN          V+   L WG
Sbjct: 88  VELGAGTGLVGI-VAALLGAQVTITDRKVALEFLKSNVEANLPPHIQPKAVVK--ELTWG 147

Query: 169 EAIDTELIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDS 228
           + +++   G EF+L+L +DV+Y +  +  LLQTL        +   V L+A   R+++DS
Sbjct: 148 QNLESFSPG-EFDLILGADVIYLEDTFTDLLQTLGHLC----SNNSVILLACRIRYERDS 200

Query: 229 AFFKKARKLFEVEVLHTDP 247
            F     + F V  +H DP
Sbjct: 208 NFLTMLERQFTVSKVHYDP 200

BLAST of MS010530 vs. ExPASy TrEMBL
Match: A0A6J1BUP4 (protein N-lysine methyltransferase METTL21A-like OS=Momordica charantia OX=3673 GN=LOC111005878 PE=4 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.0e-148
Identity = 273/279 (97.85%), Postives = 274/279 (98.21%), Query Frame = 0

Query: 1   MSAEHD---GGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL 60
           MSAEHD   GGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL
Sbjct: 20  MSAEHDGGGGGGGGDDICPISVILAEEEIDKLSLLHNGVASELQHHFIRSINSTLAIRQL 79

Query: 61  PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLV 120
           PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALR+GSDRRTLEVLEIGSGTGLV
Sbjct: 80  PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALRDGSDRRTLEVLEIGSGTGLV 139

Query: 121 GIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGR 180
           GIAAAATLGA VTVTDLSHVIPNLQFNVEANAEVLAANGG VQVAPLHWGEAIDTELIGR
Sbjct: 140 GIAAAATLGAIVTVTDLSHVIPNLQFNVEANAEVLAANGGRVQVAPLHWGEAIDTELIGR 199

Query: 181 EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF 240
           EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF
Sbjct: 200 EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF 259

Query: 241 EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG
Sbjct: 260 EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 298

BLAST of MS010530 vs. ExPASy TrEMBL
Match: A0A6J1GEP3 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111453506 PE=4 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 7.4e-126
Identity = 233/278 (83.81%), Postives = 256/278 (92.09%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  DDI PISVIL  E   I+KLSL ++GV SELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDADDISPISVILPAEAAGINKLSLPNDGVVSELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTP LTA R+GS+RRTL+VLEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTPILTASRDGSERRTLKVLEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAID ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIDPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPL+QTLR FLLGGGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDPLIQTLRGFLLGGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKPAIGSSNQG 277

BLAST of MS010530 vs. ExPASy TrEMBL
Match: A0A6J1IMY8 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111478491 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 9.7e-126
Identity = 233/278 (83.81%), Postives = 257/278 (92.45%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  DDI PISVIL  EE  I+KLSL ++GVASELQHHFIRSINSTL+IRQLP
Sbjct: 1   MSSELD-GGDDDDISPISVILPAEEAGINKLSLPNDGVASELQHHFIRSINSTLSIRQLP 60

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTPTLTA R+GS+RRTL++LEIGSGTGLVG
Sbjct: 61  SQGLSFQLWPAATTLVNLLDDQRSHPETNPLTPTLTASRDGSERRTLKILEIGSGTGLVG 120

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGA+VTVTDLSHVI NLQFNVEANA +LAANGG VQVAPLHWGEAI  ELIGRE
Sbjct: 121 IAAAATLGAQVTVTDLSHVISNLQFNVEANASILAANGGFVQVAPLHWGEAIYPELIGRE 180

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPL+QTL  FLLGGGNPKMVF+MAHLRRWKKDS FF+KARKLFE
Sbjct: 181 FDLILASDVVYHDHLYDPLIQTLSGFLLGGGNPKMVFVMAHLRRWKKDSGFFRKARKLFE 240

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           VEVLHTDPPPPG+RIGVVVYRFAA+QSKK A+GS+N+G
Sbjct: 241 VEVLHTDPPPPGSRIGVVVYRFAAKQSKKPAIGSSNQG 277

BLAST of MS010530 vs. ExPASy TrEMBL
Match: A0A6J1HFR6 (LOW QUALITY PROTEIN: protein N-lysine methyltransferase METTL21A-like OS=Cucurbita moschata OX=3662 GN=LOC111463091 PE=4 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 8.8e-119
Identity = 222/279 (79.57%), Postives = 247/279 (88.53%), Query Frame = 0

Query: 1   MSAEHDGG-GGGDDICPISVILAEEEID--KLSLLHNGVASELQHHFIRSINSTLAIRQL 60
           MS+E DGG G GDDI P++VIL EEE D  KLS++H+ + SELQ  FIRSINS+L+IRQL
Sbjct: 22  MSSEIDGGDGDGDDISPVTVILPEEEADITKLSVIHDDLTSELQRQFIRSINSSLSIRQL 81

Query: 61  PSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLV 120
            S+GLSFQLWPAATTLV LLD+QRSHPET+PLTPTLTALR  S+ RTL +LEIGSGTGLV
Sbjct: 82  RSQGLSFQLWPAATTLVNLLDKQRSHPETNPLTPTLTALRNSSECRTLNILEIGSGTGLV 141

Query: 121 GIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGR 180
           GIAAAATLGAKVTVTDLSHVI NLQFNVEANA+V+AANGGCV+ A LHWGE ID ELIG+
Sbjct: 142 GIAAAATLGAKVTVTDLSHVISNLQFNVEANADVVAANGGCVRAASLHWGEPIDAELIGQ 201

Query: 181 EFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLF 240
           EF+L+LASDVVYHDHLYDPL+QTLRSFLL GGNPKMV LMAHLRRWKKDSAF KKARK F
Sbjct: 202 EFDLILASDVVYHDHLYDPLIQTLRSFLLDGGNPKMVLLMAHLRRWKKDSAFLKKARKFF 261

Query: 241 EVEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           EV+VLHTDPP PG+RIGVVVYRFAA+QSKK A+ SA  G
Sbjct: 262 EVDVLHTDPPAPGSRIGVVVYRFAAKQSKKPAIVSAKRG 300

BLAST of MS010530 vs. ExPASy TrEMBL
Match: A0A6J1KQS9 (protein N-lysine methyltransferase METTL21A-like OS=Cucurbita maxima OX=3661 GN=LOC111497413 PE=4 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 1.1e-116
Identity = 217/278 (78.06%), Postives = 247/278 (88.85%), Query Frame = 0

Query: 1   MSAEHDGGGGGDDICPISVILAEEE--IDKLSLLHNGVASELQHHFIRSINSTLAIRQLP 60
           MS+E D GG  +DI P++VIL EEE  I+KLS++H+ + SELQ HFI SINS+L+IRQL 
Sbjct: 74  MSSELD-GGDDNDISPVTVILPEEEADINKLSVIHDDLTSELQRHFICSINSSLSIRQLR 133

Query: 61  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 120
           S+GLSFQLWPAATTLV LLD+QRSHPET+PLTPTLTALR+GS+ RTL +LEIGSGTGLVG
Sbjct: 134 SQGLSFQLWPAATTLVNLLDKQRSHPETNPLTPTLTALRDGSECRTLNILEIGSGTGLVG 193

Query: 121 IAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGRE 180
           IAAAATLGAKVT TDLSHVI NLQFN+EANA+V+AANGGCV  A LHWGEAID ELIG+E
Sbjct: 194 IAAAATLGAKVTTTDLSHVISNLQFNIEANADVVAANGGCVMAASLHWGEAIDPELIGQE 253

Query: 181 FELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFE 240
           F+L+LASDVVYHDHLYDPLLQTLRSFLL GGNPKMV +MAHLRRWKKDSAFFKKAR+ FE
Sbjct: 254 FDLILASDVVYHDHLYDPLLQTLRSFLLDGGNPKMVLVMAHLRRWKKDSAFFKKARQFFE 313

Query: 241 VEVLHTDPPPPGARIGVVVYRFAARQSKKSAVGSANEG 277
           V V+HTDPP PG+RIGVVVYRFAA+QSKK A+ S+  G
Sbjct: 314 VHVVHTDPPAPGSRIGVVVYRFAAKQSKKPAIVSSKRG 350

BLAST of MS010530 vs. TAIR 10
Match: AT5G49560.1 (Putative methyltransferase family protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.0e-75
Identity = 150/254 (59.06%), Postives = 187/254 (73.62%), Query Frame = 0

Query: 19  VILAEEEIDKLSL----LHNGVASELQHHFIRSINSTLAIRQLPSRGLSFQLWPAATTLV 78
           +++ E  +D  SL    L +G A ELQ + I SI ST+ IR+L S+GLSFQLWPAA+T V
Sbjct: 21  LLIGENGLDGESLPPTPLRDG-ALELQKYNISSIESTVVIRELTSQGLSFQLWPAASTFV 80

Query: 79  KLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVGIAAAATLGAKVTVTDL 138
            LLD  R  P  SPLT TL +L++ S    L +LE+GSGTGLVGIAAA TL A VTVTDL
Sbjct: 81  TLLDNYRRDPSKSPLTATLLSLKKPS---PLNILELGSGTGLVGIAAAITLSANVTVTDL 140

Query: 139 SHVIPNLQFNVEANAEVLAANGGCVQVAPLHWGEAIDTELIGREFELVLASDVVYHDHLY 198
            HV+ NL FN EANAE++   GG V VAPL WGEA D E++G+  +L+LASDVVYHDHLY
Sbjct: 141 PHVLDNLNFNAEANAEIVERFGGKVNVAPLRWGEADDVEVLGQNVDLILASDVVYHDHLY 200

Query: 199 DPLLQTLRSFLLGGGNPKMVFLMAHLRRWKKDSAFFKKARKLFEVEVLHTDPPPPGARIG 258
           +PLL+TLR   L G   +++FLMAHLRRWKK+S FFKKARKLF+V+V+H+D P   +RIG
Sbjct: 201 EPLLKTLRLMQLEG--KRLIFLMAHLRRWKKESVFFKKARKLFDVDVIHSDVPQESSRIG 260

Query: 259 VVVYRFAARQSKKS 269
           VVVYRF  +Q  ++
Sbjct: 261 VVVYRFTTKQPNQN 268

BLAST of MS010530 vs. TAIR 10
Match: AT3G50850.1 (Putative methyltransferase family protein )

HSP 1 Score: 266.2 bits (679), Expect = 3.0e-71
Identity = 140/230 (60.87%), Postives = 177/230 (76.96%), Query Frame = 0

Query: 36  VASELQHHFIRSINSTLAIRQLPSRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTA 95
           V SELQ + I SI ST+ IRQLP++G++F+LW  ATTLV LLD  R  P  SPL  T ++
Sbjct: 19  VESELQKYRINSIESTVVIRQLPTQGIAFKLWIPATTLVTLLDNYRRDPNISPLNRTFSS 78

Query: 96  LR-EGSDRRT-LEVLEIGSGTGLVGIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLA 155
            + +GSD  + + ++E+GSGTG+VGIAAAATLGA VTVTDL +VI NL+FN +ANA+V+A
Sbjct: 79  FQSDGSDSSSPINIVELGSGTGIVGIAAAATLGANVTVTDLPNVIENLKFNADANAQVVA 138

Query: 156 ANGGCVQVAPLHWGEAIDTELIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKM 215
             GG V VA L WGE  D E +G+  +L+LASDVVYH HLY+PLL+TLR FLL  G+ + 
Sbjct: 139 KFGGKVHVASLRWGEIDDVESLGQNVDLILASDVVYHVHLYEPLLKTLR-FLLLEGSSER 198

Query: 216 VFLMAHLRRWKKDSAFFKKARKLFEVEVLHTDPPPPGARIGVVVYRFAAR 264
           VFLMAHL+RWKK+S FFKKAR+ F+V+V+H D P  GARIGVVVYRFA +
Sbjct: 199 VFLMAHLKRWKKESIFFKKARRFFDVDVIHCDDPQEGARIGVVVYRFAPK 247

BLAST of MS010530 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 5.1e-10
Identity = 48/166 (28.92%), Postives = 75/166 (45.18%), Query Frame = 0

Query: 59  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 118
           S+ L   +W A+    K L +       S            S  +    +E+G+G G+ G
Sbjct: 31  SKHLGTTVWDASMVFAKYLGKNSRKGRFS-----------SSKLKGKRAIELGAGCGVAG 90

Query: 119 IAAAATLGAKVTVTDLSHVIPNLQFNVEAN-AEVLAANG----GCVQVAPLHWGEAIDTE 178
             A A LG  V  TD   V+P L+ NVE N + ++  N     G ++VA L WG      
Sbjct: 91  F-ALAMLGCDVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHIT 150

Query: 179 LIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAH 220
            +   F+ V+ +DVVY + L +PLL+T    +L    PK   ++ +
Sbjct: 151 AVEPPFDYVIGTDVVYSEQLLEPLLRT----ILALSGPKTTVMLGY 180

BLAST of MS010530 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 5.1e-10
Identity = 48/166 (28.92%), Postives = 75/166 (45.18%), Query Frame = 0

Query: 59  SRGLSFQLWPAATTLVKLLDEQRSHPETSPLTPTLTALREGSDRRTLEVLEIGSGTGLVG 118
           S+ L   +W A+    K L +       S            S  +    +E+G+G G+ G
Sbjct: 38  SKHLGTTVWDASMVFAKYLGKNSRKGRFS-----------SSKLKGKRAIELGAGCGVAG 97

Query: 119 IAAAATLGAKVTVTDLSHVIPNLQFNVEAN-AEVLAANG----GCVQVAPLHWGEAIDTE 178
             A A LG  V  TD   V+P L+ NVE N + ++  N     G ++VA L WG      
Sbjct: 98  F-ALAMLGCDVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHIT 157

Query: 179 LIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAH 220
            +   F+ V+ +DVVY + L +PLL+T    +L    PK   ++ +
Sbjct: 158 AVEPPFDYVIGTDVVYSEQLLEPLLRT----ILALSGPKTTVMLGY 187

BLAST of MS010530 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 5.6e-09
Identity = 49/162 (30.25%), Postives = 74/162 (45.68%), Query Frame = 0

Query: 106 EVLEIGSGTGLVGIAAAATLGAKVTVTDLSHVIPNLQFNVEANAEVLAANGGCVQVAPLH 165
           +++E+GSG GLVG   AA LG    +TDL   +  L+ N++ N       G  + V  L 
Sbjct: 148 KIVELGSGCGLVG-CIAALLGGNAVLTDLPDRLRLLKKNIQTNLHRGNTRGSAI-VQELV 207

Query: 166 WGEAIDTELIGREFELVLASDVVYHDHLYDPLLQTLRSFLLGGGNPKMVFLMAHLRRWKK 225
           WG+  D +LI    + VL SDV+Y +     L++TL        +   +FL   LR    
Sbjct: 208 WGDDPDPDLIEPFPDYVLGSDVIYSEEAVHHLVKTLLQLC---SDQTTIFLSGELRNDAV 267

Query: 226 DSAFFKKARKLFEVEVLHTDPPPPGARIGVVVYRFAARQSKK 268
              F + A K F +  +      P  R   VV     ++SK+
Sbjct: 268 LEYFLETALKDFAIGRVEQTQWHPDYRSHRVVLYVLEKKSKR 304