MS010505 (gene) Bitter gourd (TR) v1

BLAST of MS010505 vs. NCBI nr
Match: XP_022133379.1 (uncharacterized protein LOC111005966 [Momordica charantia])

HSP 1 Score: 605.1 bits (1559), Expect = 3.3e-169
Identity = 313/329 (95.14%), Postives = 313/329 (95.14%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW
Sbjct: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV
Sbjct: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDELQEFTDKLIEEEELSEDQKDAFK                AREARKKVLQEMSE
Sbjct: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFKEFVKEKVREAKKANREAREARKKVLQEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETKAAFEKMRFYKFYPVQTQDSPDISNVK
Sbjct: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 329

BLAST of MS010505 vs. NCBI nr
Match: KAF3440453.1 (hypothetical protein FNV43_RR18737 [Rhamnella rubrinervis])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 268/331 (80.97%), Postives = 290/331 (87.61%), Query Frame = 0

Query: 1   MRKGTKRKAS-KNEDAKFAEKENRKESTTATAATRAKRVKASK-PDSQPEYFQDKRNLED 60
           MRKG KRKAS K E+   A+  ++++  ++ A TRAKRVKASK P  +PEYF+DKRNLED
Sbjct: 1   MRKGAKRKASTKKEEGNSAQDNHKQQQQSSKATTRAKRVKASKPPQPEPEYFEDKRNLED 60

Query: 61  LWKAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGES 120
           LWK  FPVGTEWDQLDSVYQ+NW+FSNLE AFEEGGKLYGEKVYLFG TEPQLV  KGE+
Sbjct: 61  LWKVTFPVGTEWDQLDSVYQFNWDFSNLEQAFEEGGKLYGEKVYLFGCTEPQLVPVKGEN 120

Query: 121 RVICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLK 180
           +VICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKM WVPYIPLE RDS+VD+LK
Sbjct: 121 KVICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMDWVPYIPLEKRDSQVDRLK 180

Query: 181 SQIFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIF 240
           SQIFILSCTQRRAALKHLKIDR+KKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIF
Sbjct: 181 SQIFILSCTQRRAALKHLKIDRIKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIF 240

Query: 241 CEFDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEM 300
           CEFDWELDEL+EFTDKLI+EEELSEDQKDAFK                AREARKK L+EM
Sbjct: 241 CEFDWELDELEEFTDKLIQEEELSEDQKDAFKAFVREKVKEAKKANREAREARKKALEEM 300

Query: 301 SEETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           SEETKAAFEKMRFYKFYPVQT D+PD+SNVK
Sbjct: 301 SEETKAAFEKMRFYKFYPVQTPDTPDVSNVK 331

BLAST of MS010505 vs. NCBI nr
Match: KAG7987386.1 (hypothetical protein I3843_03G131400 [Carya illinoinensis])

HSP 1 Score: 520.4 bits (1339), Expect = 1.1e-143
Identity = 269/329 (81.76%), Postives = 289/329 (87.84%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKG KRKAS+ E AK   + +R+ES   T  ++AKRVKASKP+S+PEY +DKRNLEDLW
Sbjct: 1   MRKGAKRKASQKEGAKPEHESHREESKKTT--SQAKRVKASKPESEPEYIEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           K AFPVGTEWDQLDSVYQ NWNFSNLEDAFEEGGKL+G+K YLFG TEPQLVSFKGES+V
Sbjct: 61  KEAFPVGTEWDQLDSVYQVNWNFSNLEDAFEEGGKLHGKKAYLFGCTEPQLVSFKGESKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQRE+EEIIPMKQMKM WVPYIPLE+R S+VDKL S+
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQRESEEIIPMKQMKMDWVPYIPLENRGSQVDKLHSE 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFP EPKPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPGEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLI+EEELSEDQKDAFK                AREARKK L+EMSE
Sbjct: 241 FDWELDELEEFTDKLIQEEELSEDQKDAFKEFVKERVREAKKANREAREARKKALEEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETKAAFE MRFYKFYPVQT D+PDISNVK
Sbjct: 301 ETKAAFENMRFYKFYPVQTPDTPDISNVK 327

BLAST of MS010505 vs. NCBI nr
Match: XP_042971303.1 (protein HEAT INTOLERANT 4 isoform X1 [Carya illinoinensis] >KAG2716492.1 hypothetical protein I3760_03G129100 [Carya illinoinensis] >KAG6660879.1 hypothetical protein CIPAW_03G135000 [Carya illinoinensis] >KAG6721837.1 hypothetical protein I3842_03G131600 [Carya illinoinensis])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 268/329 (81.46%), Postives = 289/329 (87.84%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKG KRKAS+ E AK   + +R+ES   T  ++AKRVKASKP+S+PEY +DKRNLEDLW
Sbjct: 1   MRKGAKRKASQKEGAKPEHESHREESKKTT--SQAKRVKASKPESEPEYIEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           K AFPVGTEWDQLDSVYQ NWNFSNLEDAFEEGGKL+G+K YLFG TEPQLVSFKGES+V
Sbjct: 61  KEAFPVGTEWDQLDSVYQVNWNFSNLEDAFEEGGKLHGKKAYLFGCTEPQLVSFKGESKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQRE+EEIIPMKQMKM WVPYIPLE+R S+VDKL S+
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQRESEEIIPMKQMKMDWVPYIPLENRGSQVDKLHSE 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFP EPKPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPGEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLI+EEELSEDQKDAFK                AREARKK L+EMSE
Sbjct: 241 FDWELDELEEFTDKLIQEEELSEDQKDAFKEFVKERVREAKKANREAREARKKALEEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           +TKAAFE MRFYKFYPVQT D+PDISNVK
Sbjct: 301 DTKAAFENMRFYKFYPVQTPDTPDISNVK 327

BLAST of MS010505 vs. NCBI nr
Match: KAF3965322.1 (hypothetical protein CMV_010480 [Castanea mollissima])

HSP 1 Score: 519.2 bits (1336), Expect = 2.4e-143
Identity = 266/329 (80.85%), Postives = 293/329 (89.06%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKG KRKAS+ E+AK   +  ++ES  AT  ++AKRVKASKP+++PEYF+DKRNLEDLW
Sbjct: 1   MRKGAKRKASQKEEAK--PQAQQQESKKAT--SQAKRVKASKPETEPEYFEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           K  FPVGTEWDQLD+VYQ+NWNFSNLEDAFEEGGKLYG+KVYLFG TEPQLVSFKGES+V
Sbjct: 61  KEVFPVGTEWDQLDAVYQFNWNFSNLEDAFEEGGKLYGKKVYLFGCTEPQLVSFKGESKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKM WVPYIPLEDRDS+VD+LKSQ
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMDWVPYIPLEDRDSQVDRLKSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           I+IL CTQRRAALKHLKIDR+KKYEYCLPYFYQPFKEDELEQSTEVQIIFP EPKPIFCE
Sbjct: 181 IYILRCTQRRAALKHLKIDRLKKYEYCLPYFYQPFKEDELEQSTEVQIIFPVEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLI+EEEL+EDQKDAFK                AREARKK L+EM+E
Sbjct: 241 FDWELDELEEFTDKLIQEEELAEDQKDAFKEFVKEKVREAKKANREAREARKKALEEMTE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           E+KAAFE MRFYKFYPVQT DSPD+S+VK
Sbjct: 301 ESKAAFENMRFYKFYPVQTPDSPDVSSVK 325

BLAST of MS010505 vs. ExPASy Swiss-Prot
Match: A2RVJ8 (Protein HEAT INTOLERANT 4 OS=Arabidopsis thaliana OX=3702 GN=HIT4 PE=1 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 1.9e-111
Identity = 199/328 (60.67%), Postives = 255/328 (77.74%), Query Frame = 0

Query: 2   RKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLWK 61
           R+G KRK +  +D +   K+ +K         +AK+ +A+K   +P YF++KR+LEDLWK
Sbjct: 101 RRGGKRKRATKKDTEI--KDEKK------PVPKAKKPRAAKVKEEPVYFEEKRSLEDLWK 160

Query: 62  AAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRVI 121
            AFPVGTEWDQLD++Y++NW+F NLE+A EEGGKLYG+KVY+FG TEPQLV +KG ++++
Sbjct: 161 VAFPVGTEWDQLDALYEFNWDFQNLEEALEEGGKLYGKKVYVFGCTEPQLVPYKGANKIV 220

Query: 122 CIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQI 181
            +P VV + SPFPPSDKIGI SVQRE EEIIPMK+MKM W+PYIP+E RD +VDK+ SQI
Sbjct: 221 HVPAVVVIESPFPPSDKIGITSVQREVEEIIPMKKMKMDWLPYIPIEKRDRQVDKMNSQI 280

Query: 182 FILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCEF 241
           F L CTQRR+AL+H+K D++KK+EYCLPYFYQPFKEDELEQSTEVQI+FP+EP P+ CEF
Sbjct: 281 FTLGCTQRRSALRHMKEDQLKKFEYCLPYFYQPFKEDELEQSTEVQIMFPSEP-PVVCEF 340

Query: 242 DWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSEE 301
           DWE DELQEF DKL+EEE L  +Q D FK                A++ARKK ++EMSE+
Sbjct: 341 DWEFDELQEFVDKLVEEEALPAEQADEFKEYVKEQVRAAKKANREAKDARKKAIEEMSED 400

Query: 302 TKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           TK AF+KM+FYKFYP  + D+PD+S V+
Sbjct: 401 TKQAFQKMKFYKFYPQPSPDTPDVSGVQ 419

BLAST of MS010505 vs. ExPASy TrEMBL
Match: A0A6J1BVU0 (uncharacterized protein LOC111005966 OS=Momordica charantia OX=3673 GN=LOC111005966 PE=4 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.6e-169
Identity = 313/329 (95.14%), Postives = 313/329 (95.14%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW
Sbjct: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV
Sbjct: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDELQEFTDKLIEEEELSEDQKDAFK                AREARKKVLQEMSE
Sbjct: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFKEFVKEKVREAKKANREAREARKKVLQEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETKAAFEKMRFYKFYPVQTQDSPDISNVK
Sbjct: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 329

BLAST of MS010505 vs. ExPASy TrEMBL
Match: A0A2I4FW82 (protein HEAT INTOLERANT 4-like OS=Juglans regia OX=51240 GN=LOC109002555 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 4.4e-143
Identity = 268/329 (81.46%), Postives = 289/329 (87.84%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKG KRKAS+ E AK   + +R+ES   T  +RAKRVKASKP+S+PEY +DKRNLEDLW
Sbjct: 1   MRKGAKRKASQKEGAKPELESHREESKKTT--SRAKRVKASKPESEPEYIEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           K AFPVGTEWDQLDSVYQ NWNFSNLEDAFEEGGKL+G+KVYLFG TEPQLVSFKGES+V
Sbjct: 61  KEAFPVGTEWDQLDSVYQVNWNFSNLEDAFEEGGKLHGKKVYLFGCTEPQLVSFKGESKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIGINSVQRE+EEIIPMKQMKM WVPYIPLE+R S+VDKL S+
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGINSVQRESEEIIPMKQMKMDWVPYIPLENRGSQVDKLHSE 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFP EPKPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPGEPKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLI+EEELSEDQK+ FK                AREARKK ++EMSE
Sbjct: 241 FDWELDELEEFTDKLIQEEELSEDQKNTFKEFVKEKVREAKKANREAREARKKAVEEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETKAAFE MRFYKFYPVQT D+PDISNVK
Sbjct: 301 ETKAAFETMRFYKFYPVQTPDTPDISNVK 327

BLAST of MS010505 vs. ExPASy TrEMBL
Match: A0A5N6QIW9 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_002744 PE=4 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 2.9e-142
Identity = 263/329 (79.94%), Postives = 289/329 (87.84%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKG KRKAS+   AK AE    K+  +  A +RAKRVKAS P+S+PEYF+DKRNLEDLW
Sbjct: 1   MRKGAKRKASQ---AKEAETAQEKQQESKKATSRAKRVKASVPESEPEYFEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KAAFPVGTEWDQLD+VYQ+ WNFSNLEDAFEEGGKL+G+KVYLFG TEPQLVSFKGES++
Sbjct: 61  KAAFPVGTEWDQLDAVYQFKWNFSNLEDAFEEGGKLHGKKVYLFGCTEPQLVSFKGESKI 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAVVSPFPPSDKIG+NSVQREAEEIIPMKQMKM WVPYIPLE+R S+V+ L+SQ
Sbjct: 121 ICIPVVVAVVSPFPPSDKIGVNSVQREAEEIIPMKQMKMDWVPYIPLENRGSQVESLRSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDR+KKYEYCLPYFYQPFKEDELEQSTEVQIIFP++ KPIFCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRLKKYEYCLPYFYQPFKEDELEQSTEVQIIFPSDTKPIFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLI+EEEL+EDQKDAFK                AREARKK L+EMSE
Sbjct: 241 FDWELDELEEFTDKLIQEEELAEDQKDAFKEFVKEKVREAKRANREAREARKKALEEMSE 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETKAAFE MRFYKFYPVQT DSPD+SNVK
Sbjct: 301 ETKAAFENMRFYKFYPVQTPDSPDVSNVK 326

BLAST of MS010505 vs. ExPASy TrEMBL
Match: A0A6J1C502 (uncharacterized protein LOC111008415 OS=Momordica charantia OX=3673 GN=LOC111008415 PE=4 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.4e-141
Identity = 263/329 (79.94%), Postives = 285/329 (86.63%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKGTKRK ++ E+ K  E + RKE     A +RAKR K  KP+S+PEYF+DKRNLEDLW
Sbjct: 1   MRKGTKRKTARKEEDKPVEPK-RKE-----APSRAKRAKLPKPESEPEYFEDKRNLEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KAAFPVGTEWDQLD+VYQ+NWNFSNLEDAFEEGGKLYGEKVYLFG TEPQLV FKGE++V
Sbjct: 61  KAAFPVGTEWDQLDTVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIP VVAVVSPFPPSDKIGINSVQREAEEI+PMKQMKM WVPYIPLE R+SRVDKLKSQ
Sbjct: 121 ICIPAVVAVVSPFPPSDKIGINSVQREAEEIVPMKQMKMDWVPYIPLEKRESRVDKLKSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDE EQSTEV IIFP +PKP+FCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDEFEQSTEVPIIFPIDPKPVFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWELDEL+EFTDKLIEEEELSE QKDAFK                AREARKK ++EMS+
Sbjct: 241 FDWELDELEEFTDKLIEEEELSESQKDAFKDFVKEKVREAKKANREAREARKKAIEEMSK 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETK AFEKM+FYKFYPVQT D+PDISNVK
Sbjct: 301 ETKEAFEKMKFYKFYPVQTPDTPDISNVK 323

BLAST of MS010505 vs. ExPASy TrEMBL
Match: A0A0A0KUL7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G037620 PE=4 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.9e-141
Identity = 262/329 (79.64%), Postives = 282/329 (85.71%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           MRKGTKRKA++ E+ K AE +  K      A +RAKR K  KP+S+PEYF+DKRN+EDLW
Sbjct: 1   MRKGTKRKAARKEEDKPAEPKPDK------APSRAKRTKLPKPESEPEYFEDKRNMEDLW 60

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KAAFPVGTEWDQLDSVYQ+NWNFSNLEDAFEEGGKLYGEKVYLFG TEPQLV FKGE++V
Sbjct: 61  KAAFPVGTEWDQLDSVYQFNWNFSNLEDAFEEGGKLYGEKVYLFGCTEPQLVPFKGENKV 120

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           ICIPVVVAV SPFPPSDKIGINSVQREAEEIIPMKQMKM WVPYIPLE RD RVDKLKSQ
Sbjct: 121 ICIPVVVAVASPFPPSDKIGINSVQREAEEIIPMKQMKMDWVPYIPLEKRDRRVDKLKSQ 180

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFILSCTQRRAALKHLKIDR+KKYEYCLPYFYQPFK+DE EQSTEV IIFP +PKP+FCE
Sbjct: 181 IFILSCTQRRAALKHLKIDRLKKYEYCLPYFYQPFKDDEFEQSTEVPIIFPVDPKPVFCE 240

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           FDWE DEL+EFTDKLIEEEELSE QKDAFK                AREARKK ++EMS 
Sbjct: 241 FDWEFDELEEFTDKLIEEEELSESQKDAFKDFVREKVREAKKANREAREARKKAIEEMSN 300

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETK AFEKM+FYKFYPVQT DSPDISNVK
Sbjct: 301 ETKEAFEKMKFYKFYPVQTPDSPDISNVK 323

BLAST of MS010505 vs. TAIR 10
Match: AT5G10010.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: nucleolus; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G64910.1); Has 33260 Blast hits to 16857 proteins in 1270 species: Archae - 88; Bacteria - 3040; Metazoa - 11915; Fungi - 3137; Plants - 1371; Viruses - 424; Other Eukaryotes - 13285 (source: NCBI BLink). )

HSP 1 Score: 403.7 bits (1036), Expect = 1.4e-112
Identity = 199/328 (60.67%), Postives = 255/328 (77.74%), Query Frame = 0

Query: 2   RKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLWK 61
           R+G KRK +  +D +   K+ +K         +AK+ +A+K   +P YF++KR+LEDLWK
Sbjct: 101 RRGGKRKRATKKDTEI--KDEKK------PVPKAKKPRAAKVKEEPVYFEEKRSLEDLWK 160

Query: 62  AAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRVI 121
            AFPVGTEWDQLD++Y++NW+F NLE+A EEGGKLYG+KVY+FG TEPQLV +KG ++++
Sbjct: 161 VAFPVGTEWDQLDALYEFNWDFQNLEEALEEGGKLYGKKVYVFGCTEPQLVPYKGANKIV 220

Query: 122 CIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQI 181
            +P VV + SPFPPSDKIGI SVQRE EEIIPMK+MKM W+PYIP+E RD +VDK+ SQI
Sbjct: 221 HVPAVVVIESPFPPSDKIGITSVQREVEEIIPMKKMKMDWLPYIPIEKRDRQVDKMNSQI 280

Query: 182 FILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCEF 241
           F L CTQRR+AL+H+K D++KK+EYCLPYFYQPFKEDELEQSTEVQI+FP+EP P+ CEF
Sbjct: 281 FTLGCTQRRSALRHMKEDQLKKFEYCLPYFYQPFKEDELEQSTEVQIMFPSEP-PVVCEF 340

Query: 242 DWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSEE 301
           DWE DELQEF DKL+EEE L  +Q D FK                A++ARKK ++EMSE+
Sbjct: 341 DWEFDELQEFVDKLVEEEALPAEQADEFKEYVKEQVRAAKKANREAKDARKKAIEEMSED 400

Query: 302 TKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           TK AF+KM+FYKFYP  + D+PD+S V+
Sbjct: 401 TKQAFQKMKFYKFYPQPSPDTPDVSGVQ 419

BLAST of MS010505 vs. TAIR 10
Match: AT5G64910.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 318.9 bits (816), Expect = 4.5e-87
Identity = 168/329 (51.06%), Postives = 227/329 (69.00%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           +R+G +++ +K         E  K+ +T  A  RAK  KA    S+PEYF++KRNLEDLW
Sbjct: 141 LRRGKRKRGTKT--------EAEKKVSTPRAKKRAKTTKAQA--SEPEYFEEKRNLEDLW 200

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KA F VGTEWDQ D++ ++NW+F+NLE+A EEGG+LYG++VY+FG TE   V++K E++ 
Sbjct: 201 KATFSVGTEWDQQDALNEFNWDFTNLEEALEEGGELYGKQVYVFGCTESHSVTYKDENKD 260

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           + +PVVV + SP PPSD+IG+ SVQ E  EII MK MKM WVPYIPLE RD +VD     
Sbjct: 261 VLVPVVVCIDSPIPPSDEIGVASVQGEVGEIIAMKTMKMAWVPYIPLEQRDRQVDNKNFP 320

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFIL CTQRR+ALKHL  DRVKK+ YCLPY   P+K D+ E+ST V+I+FP+EP P+ CE
Sbjct: 321 IFILGCTQRRSALKHLPDDRVKKFNYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECE 380

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           +DW    ++EFTD LI EE L  +QK AF+                A+EA +K  + +SE
Sbjct: 381 YDWVKSVIEEFTDSLINEEVLLPEQKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSE 440

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETK A+++MR YKFYP+ + D+P  + ++
Sbjct: 441 ETKKAYQEMRLYKFYPLPSPDTPHTAGIE 458

BLAST of MS010505 vs. TAIR 10
Match: AT5G64910.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G10010.1). )

HSP 1 Score: 312.8 bits (800), Expect = 3.2e-85
Identity = 167/329 (50.76%), Postives = 226/329 (68.69%), Query Frame = 0

Query: 1   MRKGTKRKASKNEDAKFAEKENRKESTTATAATRAKRVKASKPDSQPEYFQDKRNLEDLW 60
           +R+G +++ +K         E  K+ +T  A  RAK  KA    S+PEYF++KRNLEDLW
Sbjct: 141 LRRGKRKRGTKT--------EAEKKVSTPRAKKRAKTTKAQA--SEPEYFEEKRNLEDLW 200

Query: 61  KAAFPVGTEWDQLDSVYQYNWNFSNLEDAFEEGGKLYGEKVYLFGSTEPQLVSFKGESRV 120
           KA F VGTEWDQ D++ ++NW+F+NLE+A EEGG+LYG++VY+FG TE    ++K E++ 
Sbjct: 201 KATFSVGTEWDQQDALNEFNWDFTNLEEALEEGGELYGKQVYVFGCTE---FTYKDENKD 260

Query: 121 ICIPVVVAVVSPFPPSDKIGINSVQREAEEIIPMKQMKMGWVPYIPLEDRDSRVDKLKSQ 180
           + +PVVV + SP PPSD+IG+ SVQ E  EII MK MKM WVPYIPLE RD +VD     
Sbjct: 261 VLVPVVVCIDSPIPPSDEIGVASVQGEVGEIIAMKTMKMAWVPYIPLEQRDRQVDNKNFP 320

Query: 181 IFILSCTQRRAALKHLKIDRVKKYEYCLPYFYQPFKEDELEQSTEVQIIFPAEPKPIFCE 240
           IFIL CTQRR+ALKHL  DRVKK+ YCLPY   P+K D+ E+ST V+I+FP+EP P+ CE
Sbjct: 321 IFILGCTQRRSALKHLPDDRVKKFNYCLPYINNPYKVDDSEKSTVVKIMFPSEP-PVECE 380

Query: 241 FDWELDELQEFTDKLIEEEELSEDQKDAFK----------------AREARKKVLQEMSE 300
           +DW    ++EFTD LI EE L  +QK AF+                A+EA +K  + +SE
Sbjct: 381 YDWVKSVIEEFTDSLINEEVLLPEQKVAFEEFVKEKSDKAMAAYDTAQEALEKAKEGLSE 440

Query: 301 ETKAAFEKMRFYKFYPVQTQDSPDISNVK 314
           ETK A+++MR YKFYP+ + D+P  + ++
Sbjct: 441 ETKKAYQEMRLYKFYPLPSPDTPHTAGIE 455