MS010500 (gene) Bitter gourd (TR) v1

BLAST of MS010500 vs. NCBI nr
Match: XP_022132483.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 998.0 bits (2579), Expect = 2.8e-287
Identity = 489/492 (99.39%), Postives = 489/492 (99.39%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR
Sbjct: 1   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS
Sbjct: 61  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM
Sbjct: 121 YWRNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK
Sbjct: 181 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT
Sbjct: 241 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP
Sbjct: 301 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG
Sbjct: 361 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           RDFQLIPFGA RRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP
Sbjct: 421 RDFQLIPFGAGRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 480

Query: 488 RVQDLIVTPSYR 500
           RVQDLIVTP YR
Sbjct: 481 RVQDLIVTPIYR 492

BLAST of MS010500 vs. NCBI nr
Match: XP_022132586.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 776.2 bits (2003), Expect = 1.8e-220
Identity = 375/497 (75.45%), Postives = 429/497 (86.32%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWL-LRNSHK-KLPPGPKGFPILGCLHLLGKLPHRDFHRLS 67
           M  IWI+I  LLALAL+ RPWL LRNSHK KLPPGPKGFPILGCLHL GK PHRD HRLS
Sbjct: 1   MCCIWILIFHLLALALVLRPWLYLRNSHKNKLPPGPKGFPILGCLHLFGKNPHRDLHRLS 60

Query: 68  QRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPY 127
           Q YGPIMH+KLGLVHTI+VSSP AAELFLKT+DLVFA+RP SEV  +I +G K++LFAPY
Sbjct: 61  QTYGPIMHIKLGLVHTIVVSSPRAAELFLKTYDLVFANRPPSEVFTHIAYGLKNILFAPY 120

Query: 128 GSYWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTL 187
           GSYW NMRK CTLELL+SHK+ SF+S+RREEVGLL EDLREAAK+GVAVDLTSKIFSL L
Sbjct: 121 GSYWSNMRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNL 180

Query: 188 EMTCLVIFGRKYEGEEVHG-RSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKF 247
           +MTCL+IFGRKY G+EV   R FKW+++EA RLGAAPNL D IPCIAP DLQGLNRRAK 
Sbjct: 181 DMTCLMIFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKS 240

Query: 248 VNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGA 307
           V KVLDGFLESIIDEHLRE+ N TKDFVD MLDI+GSR TEYQI+RS+IKA+I DMVL  
Sbjct: 241 VGKVLDGFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLAT 300

Query: 308 VDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRL 367
            DT+ATT+ W L EL+KHP+VMKKLQEELE VVGL+  VEESDL ++KYLDMIVKE LRL
Sbjct: 301 TDTTATTIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRL 360

Query: 368 HPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVD 427
           HPPGP+L PHES EDCTI+NF+IPK+SR+IVNAWAI RDPSVW + EKFFPERFI ++VD
Sbjct: 361 HPPGPLLVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVD 420

Query: 428 LKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGL 487
           +KG+ FQLIPFGA RR CPG+ M L +VH++IAQLVHCFDWKLP  +LP ELD++E+ GL
Sbjct: 421 MKGKHFQLIPFGAGRRGCPGMEMGLTIVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGL 480

Query: 488 TCPRVQDLIVTPSYRLH 502
           T PR Q+L+VTP YRL+
Sbjct: 481 TSPRAQNLMVTPIYRLY 497

BLAST of MS010500 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 704.9 bits (1818), Expect = 5.0e-199
Identity = 337/494 (68.22%), Postives = 407/494 (82.39%), Query Frame = 0

Query: 10  LIWIII-VPLLALALL-FRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 69
           + WI++ V L  LALL  +PWL R S  KLPPGP+GFP+ G LHLLGKLPHRD   LSQ+
Sbjct: 1   MAWILLAVALAGLALLILKPWLFR-SQNKLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQK 60

Query: 70  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 129
           YG IM+M+LG+V TIIV+SP+AAELFLKTHDLVFASRP  E   Y+ +GQK++ FA YGS
Sbjct: 61  YGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGS 120

Query: 130 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 189
           YW NMRK CTLELLS HK++SF S+RREEVGLL+  LR+AAK  V ++L+SKI SL  +M
Sbjct: 121 YWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDM 180

Query: 190 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 249
           TCL++FG+KY   E   R FK +++EA +L A PNL DFIPCIA LDLQGLNRR+K ++K
Sbjct: 181 TCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISK 240

Query: 250 VLDGFLESIIDEHLR-ESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVD 309
           + DGF E IIDEHL  +++N TKDFVD MLDI+GS+ TEYQI+RS IKA+I DM++ A+D
Sbjct: 241 IFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMD 300

Query: 310 TSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHP 369
           TSATT+GW + ELIKHPQVMKKLQEEL+ VVGL+R+VEESDL H++YLDM VKE LR+HP
Sbjct: 301 TSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHP 360

Query: 370 PGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLK 429
             P+L PHESLEDCT+N + IPK+SRIIVNAWAI +DP+ W + EKFFPERFI S+VD+K
Sbjct: 361 AAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVK 420

Query: 430 GRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTC 489
           GRDFQLIPFG+ RR CPG+HM L+VVH +IAQLVHCFDWKLP G+LP ELD+TEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTC 480

Query: 490 PRVQDLIVTPSYRL 501
           PR QDL+VTP YRL
Sbjct: 481 PRAQDLMVTPIYRL 493

BLAST of MS010500 vs. NCBI nr
Match: XP_023545010.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702.6 bits (1812), Expect = 2.5e-198
Identity = 327/493 (66.33%), Postives = 407/493 (82.56%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           M  IWIII+ + AL LLFRPW+LRN  K LPPGPKGFPILG LHLLGKLPHRD  RLSQR
Sbjct: 1   MAWIWIIII-MFALVLLFRPWVLRNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLV+TI+VSSPEAA LFLKTHDLV+ASRP +EV  ++ +G++ ++F+ YG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVYASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRK CTLELLS+ K++ F+S+RREE+ LLI++LREAAKS V V+L+SK+ SL+  M
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCL+IFG+K+E  E+  R FKW+++EA +L AA NL DFIP I+ LDLQGL +RAK VNK
Sbjct: 181 TCLMIFGKKFEDRELDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           V D F E I+DEHL      +KDFVD ML I+GS  TEYQI+RS IKA++ D++L A D+
Sbjct: 241 VCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDS 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SA T+GW + ELIKHP VMKK+Q+ELE VVGLNR VEE+DL H+ YL+M+VKE LRLHPP
Sbjct: 301 SAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLCHLTYLEMVVKEVLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GP++ PHE+++DCT+NNF IPK+SRII+N WAI R+PS WT+AEKFFPERFI S+VD+KG
Sbjct: 361 GPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           +DFQL+PFG+ RR CPG+ + L+ VHLI+A+LVHCFDWKLP G+LP +LD+TE FGL+CP
Sbjct: 421 KDFQLLPFGSGRRGCPGMQVGLVTVHLILAELVHCFDWKLPNGMLPVDLDMTEAFGLSCP 480

Query: 488 RVQDLIVTPSYRL 501
             QD++VTP YRL
Sbjct: 481 IAQDIMVTPIYRL 492

BLAST of MS010500 vs. NCBI nr
Match: XP_022950432.1 (cytochrome P450 CYP736A12-like [Cucurbita moschata])

HSP 1 Score: 700.3 bits (1806), Expect = 1.2e-197
Identity = 325/493 (65.92%), Postives = 406/493 (82.35%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           M  IWIII+ + AL LLF PWLL+N  K LPPGPKGFPILG LHLLGKLPHRD  RLSQR
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLKNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLV+TI+VSSPEAA LFLKTHDLVFASRP +EV  ++ +G++ ++F+ YG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRK CTLELLS+ K++ F+S+R+EE+ LLI++LREAAKS V V+L+SK+ SL+  M
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCL+IFG+K+E  ++  R FKW+++EA +L AA NL DFIP I+ LDLQGL +RAK VNK
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           V D F E I+DEHL      +KDFVD ML I+GS  TEYQI+RS IKA++ D++L A D+
Sbjct: 241 VCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDS 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SA T+GW + ELIKHP VMKK+Q+ELE VVGLNR VEE+DL H+ YL+M+VKE LRLHPP
Sbjct: 301 SAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GP++ PHE+++DCT+NNF IPK+SRII+N WAI R+PS WT+AEKFFPERFI S+VD+KG
Sbjct: 361 GPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           +DFQL+PFG+ RR CPG+ + L+ +HLI+AQLVHCFDWKLP G+LP +LD+TE FGL+CP
Sbjct: 421 KDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFGLSCP 480

Query: 488 RVQDLIVTPSYRL 501
             QD++VTP YRL
Sbjct: 481 IAQDIMVTPIYRL 492

BLAST of MS010500 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 1.8e-172
Identity = 285/494 (57.69%), Postives = 379/494 (76.72%), Query Frame = 0

Query: 10  LIWI-IIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRY 69
           ++WI   + LLAL  + + W  +N  K+LPPGP+GFPI G LHLLG+ P++D HRL+++Y
Sbjct: 1   MVWIWATIGLLALVHILQAW-WKNKKKRLPPGPRGFPIFGSLHLLGEFPNKDLHRLARKY 60

Query: 70  GPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSY 129
           G IM+M+LGL+ TI++SSPEAAELFLKTHDLVFASRP  E   +I  GQK+++F+ YG+Y
Sbjct: 61  GDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSEYGAY 120

Query: 130 WCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKS-GVAVDLTSKIFSLTLEM 189
           W + RK CT+ELLS+HKI+SF+S+RREEV L +E +R AA + GVAVDL+ K+ SL+++M
Sbjct: 121 WRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDM 180

Query: 190 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 249
           +C ++ G+KY  EE   R FK ++REA +L +APNL D+I  IAPLDLQG  +R K VNK
Sbjct: 181 SCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNK 240

Query: 250 VLDGFLESIIDEHLRESK-NHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVD 309
             D   E II+EHL+ +    T DFVD M+  +GS  +EY+I R  IKA++ DM++ ++D
Sbjct: 241 AFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLVASMD 300

Query: 310 TSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHP 369
           TSATT+ W L EL++HP+ MKK+Q+ELENVVGL++ VEESDL  + YL+M+VKET RLHP
Sbjct: 301 TSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETFRLHP 360

Query: 370 PGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLK 429
             P+L PH S+EDCT+N + IPK+SR+++N WAI RDP+ WT+AEKF PERF  S VD++
Sbjct: 361 VAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSSVDVR 420

Query: 430 GRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTC 489
           G  FQLIPFG+ RR CPGI + L VV L++AQLVHCFDW+LP  +LP ELD+TEEFGLT 
Sbjct: 421 GNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEFGLTV 480

Query: 490 PRVQDLIVTPSYRL 501
           PR + L+  PSYRL
Sbjct: 481 PRAKHLLAIPSYRL 493

BLAST of MS010500 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 2.3e-143
Identity = 245/475 (51.58%), Postives = 334/475 (70.32%), Query Frame = 0

Query: 34  HKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRYGPIMHMKLGLVHTIIVSSPEAAELF 93
           H KLPPGP+G PI+G LH LG LPHR    L+++YGPIM M+LG V TI+VSSP+AAELF
Sbjct: 30  HHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPIMSMRLGSVPTIVVSSPQAAELF 89

Query: 94  LKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSYWCNMRKTCTLELLSSHKISSFESVR 153
           LKTHD +FASRP  +   Y+ +G   + F  YG +W N+RK   LELL+  KI+SF  +R
Sbjct: 90  LKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRNIRKFVVLELLTPAKINSFVGMR 149

Query: 154 REEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTCLVIFGRKYEGEEVHGRSFKWLLRE 213
           REE+G +++ ++EA+ +   VDL++K+ ++   MT  ++ GR  +         K ++ E
Sbjct: 150 REELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRLLLGRTKDDR----YDLKGIMNE 209

Query: 214 ASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLDGFLESIIDEHLRESK--NHTKDF 273
           A  L    N+ADF+P + PLD+QGL R+ K   K LD  LE IIDEH + S   N + DF
Sbjct: 210 ALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEFIIDEHEQNSSNGNASGDF 269

Query: 274 VDAMLDILGSRGTEYQ-----INRSDIKAVIFDMVLGAVDTSATTMGWVLFELIKHPQVM 333
           +D ML +       +      I+RS IKA++ D++  A+DTS T++ W+L ELIKHP+ M
Sbjct: 270 IDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAAIDTSDTSIEWILTELIKHPRAM 329

Query: 334 KKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPPGPILAPHESLEDCTINNFF 393
           KK QEE++ VVG++R VEE+DL +++Y+ M+VKE LRLHP  P+L PHES+ED TIN +F
Sbjct: 330 KKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRLHPVAPLLGPHESMEDITINGYF 389

Query: 394 IPKRSRIIVNAWAIARDPSVWT-NAEKFFPERFIDSKVDLKGRDFQLIPFGASRRSCPGI 453
           IPK+SR+IVN+WA+ RDP+VW+ NAE+F PERF  S VD++GRDFQL+PFG+ RR CPG+
Sbjct: 390 IPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNVDVRGRDFQLLPFGSGRRGCPGM 449

Query: 454 HMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCPRVQDLIVTPSYRL 501
            + LI V L++A+LVHCFDW LP G  P  LD+TE+FGLT PRV+ L+  P YRL
Sbjct: 450 QLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFGLTTPRVKHLLAVPKYRL 500

BLAST of MS010500 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 433.7 bits (1114), Expect = 2.8e-120
Identity = 218/484 (45.04%), Postives = 303/484 (62.60%), Query Frame = 0

Query: 14  IIVPLLALALLFRPWLLRNSHKK-----LPPGPKGFPILGCLHLLGKLPHRDFHRLSQRY 73
           I+V LL LA+    +LL+ + K+     LPP P   PI+G LH LG LPHR    L+   
Sbjct: 3   ILVSLLFLAIALTFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANEL 62

Query: 74  GPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSY 133
           GP++ + LG + T+IVS+ E AE  LKTHDL+FASRP +     IF+   DV F+PYG Y
Sbjct: 63  GPLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEY 122

Query: 134 WCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMT 193
           W  +RK C LELLS  +++S+ S+R EEVGL++E + ++  +G AV+L+  +  L+    
Sbjct: 123 WRQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTI 182

Query: 194 CLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLD-LQGLNRRAKFVNK 253
             V FG+KYEGEE     F  L  E + L  A  + D+ P  A +D L G++ R K  + 
Sbjct: 183 TRVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHG 242

Query: 254 VLDGFLESIIDEHLRESKNH------TKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMV 313
            LD F++ +ID+HL   K +       KD VD +L +         +NR+++KAVI DM 
Sbjct: 243 ELDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMF 302

Query: 314 LGAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKET 373
            G  DT+A T+ W + ELIKHP VM+K Q+E+  VVG   KVEE DL  + YL +I+KET
Sbjct: 303 SGGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKET 362

Query: 374 LRLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDS 433
           LRLHP  P+L P ES  D  I  + IP ++R+ +NAWAI RDP  W NAE+F PERF+++
Sbjct: 363 LRLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNN 422

Query: 434 KVDLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEE 486
            VD KG+DFQLIPFGA RR CPGI   +  V + +A L++ F+W+LPG +   +LD++E 
Sbjct: 423 SVDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEA 482

BLAST of MS010500 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 2.8e-112
Identity = 208/496 (41.94%), Postives = 311/496 (62.70%), Query Frame = 0

Query: 25  FRPWLLRNS--HKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRYGPIMHMKLGLVHTI 84
           F  W+L  S  +++LPPGP  +PI+G  H +    HR    L+++YGPI+ ++ G V T+
Sbjct: 28  FSCWILHQSQRNERLPPGPYPWPIIGNFHQVRLPLHRTLKNLAEKYGPILFLRFGSVPTV 87

Query: 85  IVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSYWCNMRKTCTLELLS 144
           +VSS E A+ FLKTHDL+FASRP + V  Y F+  KD+ F+PYG +W  MRK C LELL+
Sbjct: 88  VVSSSEKAKHFLKTHDLIFASRPPTSVGKYFFYNFKDIAFSPYGDHWRKMRKICVLELLT 147

Query: 145 SHKISSFESVRREEVGLLIEDLREAAKSG-VAVDLTSKIFSLTLEMTCLVIFGRKYEGEE 204
           S +I SF+ VR+EE+  +I  + E ++SG +AV+++  I +    +   ++  +K+   +
Sbjct: 148 SKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWRILARKKFSDND 207

Query: 205 V--HGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLDGFLESIIDE 264
           +   G+ F  L+ E S    + N+ DFIP +  LDLQG+ R  K  N   D F E +IDE
Sbjct: 208 LGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANARFDAFAEKMIDE 267

Query: 265 HLRES--KN-------HTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDTSAT 324
           H+  S  +N       H KD +D +L++  +  T  ++ R  IKA+ +++    ++TSA 
Sbjct: 268 HINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITYELFSAGMETSAN 327

Query: 325 TMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPPGPI 384
            + W + EL++HP  MKKLQ+E+E+VVG    V+ESDL  + YL  +VKETLRL+P  P+
Sbjct: 328 VLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPSLPL 387

Query: 385 LAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVW-TNAEKFFPERFI---DSKVDLK 444
             PHESLE  T+  ++IPK++ +I+N WAI RDPSVW  +A +F PERF+   ++ +DL 
Sbjct: 388 ALPHESLEAVTVGGYYIPKKTMVIMNLWAIGRDPSVWGADASEFKPERFMQMEENGIDLS 447

Query: 445 G--RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGL 501
           G   DF+++PFGA RR+CPG  MA++ V   +AQL+H FDW++ G   P+ELD+ E    
Sbjct: 448 GGQSDFRMLPFGAGRRTCPGSAMAILTVEFTLAQLLHTFDWRVEGD--PSELDMKEACAT 507

BLAST of MS010500 vs. ExPASy Swiss-Prot
Match: Q42600 (Cytochrome P450 84A1 OS=Arabidopsis thaliana OX=3702 GN=CYP84A1 PE=1 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 7.9e-107
Identity = 198/509 (38.90%), Postives = 311/509 (61.10%), Query Frame = 0

Query: 13  IIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRYGPIM 72
           +I+V L     +F  ++ R      PPGP+G+PI+G + ++ +L HR    L+++YG + 
Sbjct: 20  VIVVSL----FIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLC 79

Query: 73  HMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSYWCNM 132
           H+++G +H   VSSPE A   L+  D VF++RP +  I+Y+ + + D+ FA YG +W  M
Sbjct: 80  HLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQM 139

Query: 133 RKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTCLVI 192
           RK C +++ S  +  S+ SV R+EV  ++  +  +   G  +++  +IF+LT  +T    
Sbjct: 140 RKVCVMKVFSRKRAESWASV-RDEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRAA 199

Query: 193 FGRKYE-GEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLDG 252
           FG   E G++     F  +L+E S+L  A N+ADFIP    +D QG+N+R       LDG
Sbjct: 200 FGSACEKGQD----EFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDG 259

Query: 253 FLESIIDEHLRESKNHT---------KDFVDAMLDILGSRG----------TEYQINRSD 312
           F++ IIDEH+++ +N            D VD +L                    ++ R +
Sbjct: 260 FIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDN 319

Query: 313 IKAVIFDMVLGAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMK 372
           IKA+I D++ G  +T A+ + W L EL++ P+ +K++Q+EL  VVGL+R+VEESD+  + 
Sbjct: 320 IKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLT 379

Query: 373 YLDMIVKETLRLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEK 432
           YL   +KETLR+HPP P+L  HE+ ED +I+ FFIPK+SR+++NA+AI RDP+ WT+ + 
Sbjct: 380 YLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDT 439

Query: 433 FFPERFIDSKV-DLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGI 492
           F P RF++  V D KG +F+ IPFG+ RRSCPG+ + L  + L +A ++HCF WKLP G+
Sbjct: 440 FRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGM 499

Query: 493 LPTELDITEEFGLTCPRVQDLIVTPSYRL 501
            P+ELD+ + FGLT P+   L   P+ RL
Sbjct: 500 KPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of MS010500 vs. ExPASy TrEMBL
Match: A0A6J1BWD4 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005329 PE=3 SV=1)

HSP 1 Score: 998.0 bits (2579), Expect = 1.4e-287
Identity = 489/492 (99.39%), Postives = 489/492 (99.39%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR
Sbjct: 1   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS
Sbjct: 61  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM
Sbjct: 121 YWRNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK
Sbjct: 181 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT
Sbjct: 241 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP
Sbjct: 301 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG
Sbjct: 361 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           RDFQLIPFGA RRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP
Sbjct: 421 RDFQLIPFGAGRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 480

Query: 488 RVQDLIVTPSYR 500
           RVQDLIVTP YR
Sbjct: 481 RVQDLIVTPIYR 492

BLAST of MS010500 vs. ExPASy TrEMBL
Match: A0A6J1BSN9 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005413 PE=3 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 8.5e-221
Identity = 375/497 (75.45%), Postives = 429/497 (86.32%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWL-LRNSHK-KLPPGPKGFPILGCLHLLGKLPHRDFHRLS 67
           M  IWI+I  LLALAL+ RPWL LRNSHK KLPPGPKGFPILGCLHL GK PHRD HRLS
Sbjct: 1   MCCIWILIFHLLALALVLRPWLYLRNSHKNKLPPGPKGFPILGCLHLFGKNPHRDLHRLS 60

Query: 68  QRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPY 127
           Q YGPIMH+KLGLVHTI+VSSP AAELFLKT+DLVFA+RP SEV  +I +G K++LFAPY
Sbjct: 61  QTYGPIMHIKLGLVHTIVVSSPRAAELFLKTYDLVFANRPPSEVFTHIAYGLKNILFAPY 120

Query: 128 GSYWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTL 187
           GSYW NMRK CTLELL+SHK+ SF+S+RREEVGLL EDLREAAK+GVAVDLTSKIFSL L
Sbjct: 121 GSYWSNMRKMCTLELLNSHKVQSFKSMRREEVGLLTEDLREAAKTGVAVDLTSKIFSLNL 180

Query: 188 EMTCLVIFGRKYEGEEVHG-RSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKF 247
           +MTCL+IFGRKY G+EV   R FKW+++EA RLGAAPNL D IPCIAP DLQGLNRRAK 
Sbjct: 181 DMTCLMIFGRKYVGKEVDDERGFKWVVQEALRLGAAPNLGDLIPCIAPFDLQGLNRRAKS 240

Query: 248 VNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGA 307
           V KVLDGFLESIIDEHLRE+ N TKDFVD MLDI+GSR TEYQI+RS+IKA+I DMVL  
Sbjct: 241 VGKVLDGFLESIIDEHLREADNETKDFVDVMLDIVGSRETEYQIDRSNIKAIILDMVLAT 300

Query: 308 VDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRL 367
            DT+ATT+ W L EL+KHP+VMKKLQEELE VVGL+  VEESDL ++KYLDMIVKE LRL
Sbjct: 301 TDTTATTIEWALSELVKHPRVMKKLQEELEKVVGLSGMVEESDLDYLKYLDMIVKEILRL 360

Query: 368 HPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVD 427
           HPPGP+L PHES EDCTI+NF+IPK+SR+IVNAWAI RDPSVW + EKFFPERFI ++VD
Sbjct: 361 HPPGPLLVPHESSEDCTIDNFYIPKKSRVIVNAWAIGRDPSVWIDVEKFFPERFIGNQVD 420

Query: 428 LKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGL 487
           +KG+ FQLIPFGA RR CPG+ M L +VH++IAQLVHCFDWKLP  +LP ELD++E+ GL
Sbjct: 421 MKGKHFQLIPFGAGRRGCPGMEMGLTIVHMVIAQLVHCFDWKLPNDMLPIELDMSEKSGL 480

Query: 488 TCPRVQDLIVTPSYRLH 502
           T PR Q+L+VTP YRL+
Sbjct: 481 TSPRAQNLMVTPIYRLY 497

BLAST of MS010500 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.4e-199
Identity = 337/494 (68.22%), Postives = 407/494 (82.39%), Query Frame = 0

Query: 10  LIWIII-VPLLALALL-FRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 69
           + WI++ V L  LALL  +PWL R S  KLPPGP+GFP+ G LHLLGKLPHRD   LSQ+
Sbjct: 1   MAWILLAVALAGLALLILKPWLFR-SQNKLPPGPRGFPVFGSLHLLGKLPHRDLRDLSQK 60

Query: 70  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 129
           YG IM+M+LG+V TIIV+SP+AAELFLKTHDLVFASRP  E   Y+ +GQK++ FA YGS
Sbjct: 61  YGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQYGS 120

Query: 130 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 189
           YW NMRK CTLELLS HK++SF S+RREEVGLL+  LR+AAK  V ++L+SKI SL  +M
Sbjct: 121 YWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLNTDM 180

Query: 190 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 249
           TCL++FG+KY   E   R FK +++EA +L A PNL DFIPCIA LDLQGLNRR+K ++K
Sbjct: 181 TCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKAISK 240

Query: 250 VLDGFLESIIDEHLR-ESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVD 309
           + DGF E IIDEHL  +++N TKDFVD MLDI+GS+ TEYQI+RS IKA+I DM++ A+D
Sbjct: 241 IFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVAAMD 300

Query: 310 TSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHP 369
           TSATT+GW + ELIKHPQVMKKLQEEL+ VVGL+R+VEESDL H++YLDM VKE LR+HP
Sbjct: 301 TSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILRVHP 360

Query: 370 PGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLK 429
             P+L PHESLEDCT+N + IPK+SRIIVNAWAI +DP+ W + EKFFPERFI S+VD+K
Sbjct: 361 AAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQVDVK 420

Query: 430 GRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTC 489
           GRDFQLIPFG+ RR CPG+HM L+VVH +IAQLVHCFDWKLP G+LP ELD+TEEFGLTC
Sbjct: 421 GRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFGLTC 480

Query: 490 PRVQDLIVTPSYRL 501
           PR QDL+VTP YRL
Sbjct: 481 PRAQDLMVTPIYRL 493

BLAST of MS010500 vs. ExPASy TrEMBL
Match: A0A6J1GET0 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111453535 PE=3 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 5.9e-198
Identity = 325/493 (65.92%), Postives = 406/493 (82.35%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           M  IWIII+ + AL LLF PWLL+N  K LPPGPKGFPILG LHLLGKLPHRD  RLSQR
Sbjct: 1   MAWIWIIII-MFALVLLFWPWLLKNKGKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLV+TI+VSSPEAA LFLKTHDLVFASRP +EV  ++ +G++ ++F+ YG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPTNEVFKHMSYGERSMVFSKYGH 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRK CTLELLS+ K++ F+S+R+EE+ LLI++LREAAKS V V+L+SK+ SL+  M
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRKEELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCL+IFG+K+E  ++  R FKW+++EA +L AA NL DFIP I+ LDLQGL +RAK VNK
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           V D F E I+DEHL      +KDFVD ML I+GS  TEYQI+RS IKA++ D++L A D+
Sbjct: 241 VCDRFFERILDEHLESKNEDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDS 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SA T+GW + ELIKHP VMKK+Q+ELE VVGLNR VEE+DL H+ YL+M+VKE LRLHPP
Sbjct: 301 SAVTIGWAVAELIKHPHVMKKVQDELEKVVGLNRMVEETDLSHLTYLEMVVKEVLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GP++ PHE+++DCT+NNF IPK+SRII+N WAI R+PS WT+AEKFFPERFI S+VD+KG
Sbjct: 361 GPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           +DFQL+PFG+ RR CPG+ + L+ +HLI+AQLVHCFDWKLP G+LP +LD+TE FGL+CP
Sbjct: 421 KDFQLLPFGSGRRGCPGMQVGLVTIHLILAQLVHCFDWKLPNGMLPADLDMTEAFGLSCP 480

Query: 488 RVQDLIVTPSYRL 501
             QD++VTP YRL
Sbjct: 481 IAQDIMVTPIYRL 492

BLAST of MS010500 vs. ExPASy TrEMBL
Match: A0A6J1IU54 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111478446 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 4.2e-196
Identity = 322/493 (65.31%), Postives = 405/493 (82.15%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQR 67
           M  IW+II+ + AL LLFR WLL+N  K LPPGPKGFPILG LHLLGKLPHRD  RLSQR
Sbjct: 1   MAWIWMIII-MFALVLLFRSWLLKNKAKNLPPGPKGFPILGSLHLLGKLPHRDIQRLSQR 60

Query: 68  YGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGS 127
           YGPIMHMKLGLV+TI+VSSPEAA LFLKTHDLVFASRP ++V  ++ +G++ ++F+ YG 
Sbjct: 61  YGPIMHMKLGLVNTIVVSSPEAAALFLKTHDLVFASRPQNQVFKHMSYGERSMVFSKYGH 120

Query: 128 YWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEM 187
           YW NMRK CTLELLS+ K++ F+S+RREE+ LLI++LREAAKS V V+L+SK+ SL+  M
Sbjct: 121 YWRNMRKICTLELLSNQKVNFFKSMRREELSLLIDELREAAKSEVVVNLSSKVCSLSANM 180

Query: 188 TCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNK 247
           TCL+IFG+K+E  ++  R FKW+++EA +L AA NL DFIP I+ LDLQGL +RAK VNK
Sbjct: 181 TCLMIFGKKFEDRDLDERGFKWVVQEALKLAAAFNLGDFIPFISALDLQGLIQRAKCVNK 240

Query: 248 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGAVDT 307
           V D F E I+DEHL    + +KDFVD ML I+GS  TEYQI+RS IKA++ D++L A D+
Sbjct: 241 VCDRFFERILDEHLESKNDDSKDFVDVMLGIMGSEVTEYQIDRSSIKAIMLDILLAATDS 300

Query: 308 SATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLHPP 367
           SA T+GW + ELIKHP VMK +Q+ELE VVGLNR VEE+DL H+ YL+++VKE LRLHPP
Sbjct: 301 SAVTIGWAVAELIKHPYVMKNVQDELEKVVGLNRMVEETDLCHLTYLEIVVKEVLRLHPP 360

Query: 368 GPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDLKG 427
           GP++ PHE+++DCT+NNF IPK+SRII+N WAI R+PS WT+AEKFFPERFI S+VD+KG
Sbjct: 361 GPLIVPHEAVKDCTVNNFHIPKKSRIIINVWAIGRNPSAWTDAEKFFPERFIGSQVDVKG 420

Query: 428 RDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLTCP 487
           +DFQL+PFG+ RR CPGI + L+ VHLI+AQL+HCFDWKLP G+LP +LD+ E FGL+CP
Sbjct: 421 KDFQLLPFGSGRRGCPGIQVGLVTVHLILAQLIHCFDWKLPNGMLPADLDMMEAFGLSCP 480

Query: 488 RVQDLIVTPSYRL 501
             QD++VTP YRL
Sbjct: 481 IAQDIMVTPIYRL 492

BLAST of MS010500 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 389.0 bits (998), Expect = 5.6e-108
Identity = 198/509 (38.90%), Postives = 311/509 (61.10%), Query Frame = 0

Query: 13  IIIVPLLALALLFRPWLLRNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRYGPIM 72
           +I+V L     +F  ++ R      PPGP+G+PI+G + ++ +L HR    L+++YG + 
Sbjct: 20  VIVVSL----FIFISFITRRRRPPYPPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLC 79

Query: 73  HMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSYWCNM 132
           H+++G +H   VSSPE A   L+  D VF++RP +  I+Y+ + + D+ FA YG +W  M
Sbjct: 80  HLRMGFLHMYAVSSPEVARQVLQVQDSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQM 139

Query: 133 RKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTCLVI 192
           RK C +++ S  +  S+ SV R+EV  ++  +  +   G  +++  +IF+LT  +T    
Sbjct: 140 RKVCVMKVFSRKRAESWASV-RDEVDKMVRSV--SCNVGKPINVGEQIFALTRNITYRAA 199

Query: 193 FGRKYE-GEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLDLQGLNRRAKFVNKVLDG 252
           FG   E G++     F  +L+E S+L  A N+ADFIP    +D QG+N+R       LDG
Sbjct: 200 FGSACEKGQD----EFIRILQEFSKLFGAFNVADFIPYFGWIDPQGINKRLVKARNDLDG 259

Query: 253 FLESIIDEHLRESKNHT---------KDFVDAMLDILGSRG----------TEYQINRSD 312
           F++ IIDEH+++ +N            D VD +L                    ++ R +
Sbjct: 260 FIDDIIDEHMKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDN 319

Query: 313 IKAVIFDMVLGAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMK 372
           IKA+I D++ G  +T A+ + W L EL++ P+ +K++Q+EL  VVGL+R+VEESD+  + 
Sbjct: 320 IKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLT 379

Query: 373 YLDMIVKETLRLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEK 432
           YL   +KETLR+HPP P+L  HE+ ED +I+ FFIPK+SR+++NA+AI RDP+ WT+ + 
Sbjct: 380 YLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDT 439

Query: 433 FFPERFIDSKV-DLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGI 492
           F P RF++  V D KG +F+ IPFG+ RRSCPG+ + L  + L +A ++HCF WKLP G+
Sbjct: 440 FRPSRFLEPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGM 499

Query: 493 LPTELDITEEFGLTCPRVQDLIVTPSYRL 501
            P+ELD+ + FGLT P+   L   P+ RL
Sbjct: 500 KPSELDMNDVFGLTAPKATRLFAVPTTRL 516

BLAST of MS010500 vs. TAIR 10
Match: AT2G30750.1 (cytochrome P450, family 71, subfamily A, polypeptide 12 )

HSP 1 Score: 382.9 bits (982), Expect = 4.0e-106
Identity = 198/499 (39.68%), Postives = 304/499 (60.92%), Query Frame = 0

Query: 5   ITKMGLIWIIIVPLLALALLF--RPWLLRNSHK-KLPPGPKGFPILGCLHLLGKLPHRDF 64
           I +M +I ++ + L  L  LF  + +L R ++K  LPP P   P++G LH L   PHR  
Sbjct: 4   IQEMEMILMVSLCLTTLITLFLLKQFLKRTANKVNLPPSPWRLPLIGNLHQLSLHPHRSL 63

Query: 65  HRLSQRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVL 124
           H LS RYGP+M +  G V  ++VSS EAA+  LKTHDL FA+RP S+ ++ + +G +DV+
Sbjct: 64  HSLSLRYGPLMLLHFGRVPILVVSSGEAAQEVLKTHDLKFANRPRSKAVHGLMNGGRDVV 123

Query: 125 FAPYGSYWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIF 184
           F PYG YW  M+  C L LL++  ++SFE +R EE+  +I+ L +A+ S  + +L+    
Sbjct: 124 FGPYGEYWRQMKSVCILNLLTNKMVASFEKIREEELNEMIKKLEKASSSSSSENLSELFV 183

Query: 185 SLTLEMTCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLD-LQGLNR 244
           +L  ++T  +  GRK+  +E   R  K  +R+   L     + D++P +A +D + G N 
Sbjct: 184 TLPSDVTSRIALGRKHSEDET-ARDLKKRVRQIMELLGEFPIGDYVPALAWIDRINGFNA 243

Query: 245 RAKFVNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDM 304
           R K V++     ++ ++ EHL E+ NH +DFVD +L I   +   +Q  R DIK +I DM
Sbjct: 244 RIKEVSQGFSDLMDKVVQEHL-EAGNHKEDFVDILLSIESEKSIGFQAQRDDIKFMILDM 303

Query: 305 VLGAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRK-VEESDLGHMKYLDMIVK 364
            +G   TS+T + W++ ELI++P VMKKLQ+E+ + +  +   ++E D+ +MKYL  ++K
Sbjct: 304 FIGGTSTSSTLLEWIMTELIRNPNVMKKLQDEIRSTIRPHGSYIKEKDVENMKYLKAVIK 363

Query: 365 ETLRLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVW-TNAEKFFPERF 424
           E  R+HPP P++ P    ED  +  + I   + +I+NAWAI RDP++W  +AE+F PER 
Sbjct: 364 EVFRVHPPLPLILPRLLSEDVKVKGYNIAAGTEVIINAWAIQRDPAIWGPDAEEFKPERH 423

Query: 425 IDSKVDLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDI 484
           +DS +D  G+D   IPFG+ RR CPGI++AL +V + +A LV  FDW+   G    + D+
Sbjct: 424 LDSTLDYHGKDLNFIPFGSGRRICPGINLALGLVEVTVANLVGRFDWRAEAGPNGDQPDL 483

Query: 485 TEEFGLTCPRVQDLIVTPS 498
           TE FGL   R   LI  PS
Sbjct: 484 TEAFGLDVCRKFPLIAFPS 500

BLAST of MS010500 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 376.3 bits (965), Expect = 3.8e-104
Identity = 189/501 (37.72%), Postives = 293/501 (58.48%), Query Frame = 0

Query: 8   MGLIWIIIVPLLALALLFRPWLLRNSHKKLP-----PGPKGFPILGCLHLLGKLPHRDFH 67
           M  IW++  PL+ L  +    L   +HKK P     P P GFPI+G LH +G+LPH+   
Sbjct: 1   MAHIWLL--PLIFLVCIL---LAVFNHKKHPKYRQFPCPPGFPIIGNLHQIGELPHQTLW 60

Query: 68  RLSQRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLF 127
           +LS++YGP+MH+ LG V T++VSS + A   L+ HDL   +RP       + +   D+ F
Sbjct: 61  KLSKKYGPVMHLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAF 120

Query: 128 APYGSYWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFS 187
           +PY  YW  +RK C  EL S+ ++ S + ++ EEV  +I+ + E+A     V+L +K   
Sbjct: 121 SPYDDYWKEVRKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLE 180

Query: 188 LTLEMTCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPL--DLQGLNR 247
           LT+ + C   FG  +EG  ++   F  ++REA  +  + + ADFIP +  +   L GL  
Sbjct: 181 LTVSVVCRTAFGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQG 240

Query: 248 RAKFVNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGT--EYQINRSDIKAVIF 307
           R +   + L+ F E + D H    K   +DFVD +L +          ++ R+ IKA++ 
Sbjct: 241 RRERSKRDLNAFFEQMFDLHKEGKKEGNEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILL 300

Query: 308 DMVLGAVDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIV 367
           D++L  +DTSA TM W + EL ++P+VMKK+Q E+   +G    +   D+  ++YL M++
Sbjct: 301 DVLLAGIDTSAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVI 360

Query: 368 KETLRLHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERF 427
           KET RLHP  P+L P E++ +  IN + IP ++R+ VN WAI RDP  W + E F PERF
Sbjct: 361 KETWRLHPTTPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERF 420

Query: 428 IDSKVDLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDI 487
           +D+ +D KG+ F+L+PFG  RR CP I+M   +V   +A L++ FDWKLP G+   ++D+
Sbjct: 421 MDNNIDAKGQHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDV 480

Query: 488 TEEFGLTCPRVQDLIVTPSYR 500
            E  GLT  +  +L++ P  R
Sbjct: 481 EEAPGLTVNKKNELLLVPEMR 496

BLAST of MS010500 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 372.9 bits (956), Expect = 4.2e-103
Identity = 190/495 (38.38%), Postives = 300/495 (60.61%), Query Frame = 0

Query: 7   KMGLIWIIIVPLLALALLFRPWLLRNSHK-KLPPGPKGFPILGCLHLLGKLPHRDFHRLS 66
           +M L+  + +  L   LL + +L R + K  LPP P   P++G LH L   PHR  H LS
Sbjct: 2   EMTLMVSLCLTTLLTLLLLKKFLKRTAKKVNLPPSPWRIPVIGNLHQLSLHPHRSLHSLS 61

Query: 67  QRYGPIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPY 126
            RYGP+M +  G V  ++VSS EAA   LKTHDL FA+RP S+ ++ + +G +DV+F PY
Sbjct: 62  LRYGPLMLLHFGRVPILVVSSSEAAHEILKTHDLKFANRPKSKAVHGLMNGGRDVVFGPY 121

Query: 127 GSYWCNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTL 186
           G YW  M+  C L LL++  ++SFE VR EEV  ++E L +A+ S  A +L+    +LT 
Sbjct: 122 GEYWRQMKSVCILNLLTNKMVASFEKVREEEVNAMMEKLEKASCSSSAENLSELFVTLTS 181

Query: 187 EMTCLVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLD-LQGLNRRAKF 246
           ++T  V  G+KY  +E  G   K  +R+   L     + D++P +A +D + G N +   
Sbjct: 182 DVTSRVSLGKKYWEDETAG-GLKKRVRQIMELLREFPIGDYVPALAWIDRINGFNSKIVE 241

Query: 247 VNKVLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGTEYQINRSDIKAVIFDMVLGA 306
           V++     +E ++ EHL E+  H  DFV+ +L I   +   +++ R+DIK +I DM +G 
Sbjct: 242 VSRAYSDLMEKVVQEHL-EAGEHKADFVNILLSIEKEKNNGFKVQRNDIKFMILDMFIGG 301

Query: 307 VDTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRK-VEESDLGHMKYLDMIVKETLR 366
           + TS+T + W++ ELI++P+ MKKLQ E+ + +  +   ++E ++ +M+YL  ++KE  R
Sbjct: 302 ISTSSTLLEWIMTELIRNPECMKKLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFR 361

Query: 367 LHPPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVW-TNAEKFFPERFIDSK 426
           +HPP P++ P    ED  +  + I   + +++NAW+I RDP++W  +AE+F PER +DS 
Sbjct: 362 VHPPLPLILPRLLTEDVKVKGYDIAAGTEVLINAWSIHRDPAIWGPDAEEFKPERHLDST 421

Query: 427 VDLKGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEF 486
           +D  G+D + IPFG+ RR CPGI++A+ +V + +A LV  FDW +  G    + D+ E+F
Sbjct: 422 LDYHGQDLKYIPFGSGRRICPGINLAMGLVEVTLANLVGRFDWSVDPGPNGDQPDLAEDF 481

Query: 487 GLTCPRVQDLIVTPS 498
           GL   R   LI  PS
Sbjct: 482 GLDVCRKNPLIAFPS 494

BLAST of MS010500 vs. TAIR 10
Match: AT3G26320.1 (cytochrome P450, family 71, subfamily B, polypeptide 36 )

HSP 1 Score: 372.9 bits (956), Expect = 4.2e-103
Identity = 177/491 (36.05%), Postives = 293/491 (59.67%), Query Frame = 0

Query: 18  LLALALLFRPWLL--------RNSHKKLPPGPKGFPILGCLHLLGKLPHRDFHRLSQRYG 77
           +L L+LLF   +L        R  H++ PP P GFPI+G LH LG+LPH+   RLS++YG
Sbjct: 4   ILFLSLLFLSCILLAAFTHKKRQQHQRKPPSPPGFPIIGNLHQLGELPHQSLWRLSKKYG 63

Query: 78  PIMHMKLGLVHTIIVSSPEAAELFLKTHDLVFASRPHSEVINYIFHGQKDVLFAPYGSYW 137
            +M +K G + T++VSS E A+  LK HDL   SRP       + +   D+ F+P+  YW
Sbjct: 64  HVMLLKFGSIPTVVVSSSETAKQVLKIHDLHCCSRPSLAGPRALSYNYLDIAFSPFDDYW 123

Query: 138 CNMRKTCTLELLSSHKISSFESVRREEVGLLIEDLREAAKSGVAVDLTSKIFSLTLEMTC 197
             +R+ C  EL S  ++ SF+ ++ +EV  LI+ + E+A  G  V+L+ K  SLT+ +TC
Sbjct: 124 KELRRICVQELFSVKRVQSFQPIKEDEVKKLIDSVSESASQGTPVNLSEKFTSLTVRVTC 183

Query: 198 LVIFGRKYEGEEVHGRSFKWLLREASRLGAAPNLADFIPCIAPLD--LQGLNRRAKFVNK 257
              FG  ++G  ++   F+ L+ +      + + +D+ P    +   L GL+ + +   +
Sbjct: 184 KATFGVNFQGTVLNSDRFEKLIHDTYLFLGSFSASDYFPNGGWIIDWLTGLHGQRERSVR 243

Query: 258 VLDGFLESIIDEHLRESKNHTKDFVDAMLDILGSRGT--EYQINRSDIKAVIFDMVLGAV 317
            LD F E + D H + +K   +DFVD +L +          ++ R+ IKA++ ++++G +
Sbjct: 244 ALDAFYEQMFDLHKQGNKEGVEDFVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGI 303

Query: 318 DTSATTMGWVLFELIKHPQVMKKLQEELENVVGLNRKVEESDLGHMKYLDMIVKETLRLH 377
            TSA TM W + EL+++P+VMKK+Q E+ N +G    +   D+  + YL M++ ET RLH
Sbjct: 304 GTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWRLH 363

Query: 378 PPGPILAPHESLEDCTINNFFIPKRSRIIVNAWAIARDPSVWTNAEKFFPERFIDSKVDL 437
           PP P L P + + +  +N++ IP ++R+ VN WAI RDP  W + E+F PERF++S +D 
Sbjct: 364 PPSPFLIPRQVMSEFELNDYVIPVKTRLYVNVWAIGRDPDTWKDPEEFLPERFVNSSIDA 423

Query: 438 KGRDFQLIPFGASRRSCPGIHMALIVVHLIIAQLVHCFDWKLPGGILPTELDITEEFGLT 497
           KG+ F+L+PFG+ RR CP ++M   +V   +A +++ FDWK+P G++  ++D+ E  GL 
Sbjct: 424 KGQHFELLPFGSGRRMCPAMYMGTTMVEFGLANMLYHFDWKIPVGMVAEDIDLEESPGLN 483