MS010491 (gene) Bitter gourd (TR) v1

Overview


Name	MS010491
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	scaffold35: 54148 .. 56441 (-)
RNA-Seq Expression	MS010491
Synteny	MS010491

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTCGCCGGAAAAGAAGTGGTTCTGATCTCCGGCTGTTCCGACGGAGGCATAGGACATGCCCTGGCTCGAGCATTTGCCGGCCACGACTGTGTGGTGGTTCCCACCAGTAGGTCCCGGCGGTCGATGGCGGAGCTGGAGCAGGATCCTAGGTTTTTCTTACAAGAACTGGATGTCCAGTCGGACGAGAGCGTGGAGCGATTAACGTCTCTCGTCATCGAAAAATTCGGTCGAATCGATATTTTGGTGAACAACGCTGGAGTTCAATGTATTGGCCCCATACCCGAAGTTCCTCTCTCAGCTATGCAGAACGCCTTCAATACCAATGTTTTTGGTGAGCTACCGAATTACTCTTTTTTTTTTTTTTCTTTTTAAATTCTGATTCATTCGTTGTGGAATTCGTTCTCCTCTTAATTTCAATCCAGCAGAATCAAATGATAACAAAACTAGGGATTTGAAATTGCAAATTGCCTGAGCCCCCAATATAAGTTCAGAATTGATTATATTTTGGGCATTATACTTGTTCATCGGTTCTTATGGATATCTTCTTGTTCTGAGATTAACATCTGATTTGATCGAATAATCAAAATTCAATCTACTTCTACGGAACCAACAGTGTCGGCTGAGTTGCTAAGTTGCTCGTTTCGTTAAAAACAAGGCTGTTGTTTGACTGTTTGTGTCAAAGTAGAAAGTACAGTAGAAATTTGGAAGAGGACGACATGTCCAGATATGAAAGGGCGGTTTTCTGAATTTGATGATATTAAATTAATATCGTGATTGATTGTACCAATTTTGGGAGTACAGGGACTATGAGATTGATACAAGCGGTGGTTCCTCACATGGCATCACGCAGAAAGGGGAAGATAGTGAATTTGGGAAGCGTGACCGTAATGGCTCCTACTCCATGGGCTGGTGCTTATACTGCGAGTAAAGCCGCTGTTCATACCATTACTGACTGTCTGAGGTAAGGTTTGTAATTTTATACCCCTATCTGATTCACATCTAAAAATAAACAATTGATGTTTTTTTTCATTATCTTGCCCACAATGTGTTTTGCCTAGATTTGTTGCTGGTCTTGTTTAATCGACGCATCTTCTTGTTGTGGACCCTAGTACAGTGCAATTTTAGGATGAACATTGTGAGATATTAGATCCTAGCGTGGTTATACTCGAAATCAAGATCTCGGTGTAATGCAAATGGGTAGAAGAACACTACGATGTTGCTGTTGTTTTAAAATCACGATGTTTATCCATCCGATAGCATTTGGGACTCTAAAACCGCGATCTTGTAACTTAGATTTGCCATATCAGCTCAATTATCTCTCGATTAGTTATTTTATGGATATGCTAGTGGAGGGAGTATTGAAGCCTATTCACGAAAAATTTAGGGGTAAAAGTGCTTAATTTTGAAACTCCAGGACCAAATAACACTAGAAAACTTAGTAACCAGAAAAGTAGGTTTCCTTTTCTTCCCTTTTATAGAAAAATTAATGTACTCTGGTGGTTGAATTTGATCTAAGTTTTTATTTCAATAAATTGAAACTTAAACTTTTTAACCAGTAGCTTCATTAATTCATTTGCCGACTATTTTATAGGTTTATTTGGGGTGTGATAATTGCTGTTGATATTTTTAGTTGGATTCGTACATTTTTAGGTAAGGTCCAATTTATTTTACTTGGTTTTGAGTCTGAGGTTGGGTCTAGTTTGATCCTGGTGTTTTTGAGCTTGATTCTGACCCGTAGTTTGGTCCCTAACTTTTCGAGTTGTATATAATAGAGCCGGAACTTTAAAAGATATTTAATATTATTCCAAATTTTTAAGTTTCAAAAAATTTAGGGGATGTATTATTTCATATTTAAGATTCAAGCAGACCTCCATTTGATGGAATGAGATTCAATTTGCATTCACCTGCTAATTATAAATATTTATTGTGATTGAAGGATGGAGGTGAAGCCATTTGGAATCGACGTGATCAATGTGGTCCCCGGTGCCATAAAATCGAACATCGGAAGCTCTGCCGTAGCTGGGTTCAATGAGATGCCGGAGCTGAAGCTGTTCAAGGCGTTCGAAGGTGCAATTGTGGCCAGAGCCAACGCCTCGCAAGGCCCCAAGTCCACCCCCACCGCCGAGTTTGCCGAGAGGACGGTGGCCGCCGTCCTCAAAAAGAGGCCGCCCCCTTGGTTCTCCTATGGCCGCTATTCCACCATTATGGCCATCTTGTACCATTTGCCGATCTCCCTCAAGGACTTTCTCATGGGTGCACTCTCTAAGAGTTCTGGGGATCACAACCAC

mRNA sequence

ATGGAGTTCGCCGGAAAAGAAGTGGTTCTGATCTCCGGCTGTTCCGACGGAGGCATAGGACATGCCCTGGCTCGAGCATTTGCCGGCCACGACTGTGTGGTGGTTCCCACCAGTAGGTCCCGGCGGTCGATGGCGGAGCTGGAGCAGGATCCTAGGTTTTTCTTACAAGAACTGGATGTCCAGTCGGACGAGAGCGTGGAGCGATTAACGTCTCTCGTCATCGAAAAATTCGGTCGAATCGATATTTTGGTGAACAACGCTGGAGTTCAATGTATTGGCCCCATACCCGAAGTTCCTCTCTCAGCTATGCAGAACGCCTTCAATACCAATGTTTTTGGGACTATGAGATTGATACAAGCGGTGGTTCCTCACATGGCATCACGCAGAAAGGGGAAGATAGTGAATTTGGGAAGCGTGACCGTAATGGCTCCTACTCCATGGGCTGGTGCTTATACTGCGAGTAAAGCCGCTGTTCATACCATTACTGACTGTCTGAGGATGGAGGTGAAGCCATTTGGAATCGACGTGATCAATGTGGTCCCCGGTGCCATAAAATCGAACATCGGAAGCTCTGCCGTAGCTGGGTTCAATGAGATGCCGGAGCTGAAGCTGTTCAAGGCGTTCGAAGGTGCAATTGTGGCCAGAGCCAACGCCTCGCAAGGCCCCAAGTCCACCCCCACCGCCGAGTTTGCCGAGAGGACGGTGGCCGCCGTCCTCAAAAAGAGGCCGCCCCCTTGGTTCTCCTATGGCCGCTATTCCACCATTATGGCCATCTTGTACCATTTGCCGATCTCCCTCAAGGACTTTCTCATGGGTGCACTCTCTAAGAGTTCTGGGGATCACAACCAC

Coding sequence (CDS)

ATGGAGTTCGCCGGAAAAGAAGTGGTTCTGATCTCCGGCTGTTCCGACGGAGGCATAGGACATGCCCTGGCTCGAGCATTTGCCGGCCACGACTGTGTGGTGGTTCCCACCAGTAGGTCCCGGCGGTCGATGGCGGAGCTGGAGCAGGATCCTAGGTTTTTCTTACAAGAACTGGATGTCCAGTCGGACGAGAGCGTGGAGCGATTAACGTCTCTCGTCATCGAAAAATTCGGTCGAATCGATATTTTGGTGAACAACGCTGGAGTTCAATGTATTGGCCCCATACCCGAAGTTCCTCTCTCAGCTATGCAGAACGCCTTCAATACCAATGTTTTTGGGACTATGAGATTGATACAAGCGGTGGTTCCTCACATGGCATCACGCAGAAAGGGGAAGATAGTGAATTTGGGAAGCGTGACCGTAATGGCTCCTACTCCATGGGCTGGTGCTTATACTGCGAGTAAAGCCGCTGTTCATACCATTACTGACTGTCTGAGGATGGAGGTGAAGCCATTTGGAATCGACGTGATCAATGTGGTCCCCGGTGCCATAAAATCGAACATCGGAAGCTCTGCCGTAGCTGGGTTCAATGAGATGCCGGAGCTGAAGCTGTTCAAGGCGTTCGAAGGTGCAATTGTGGCCAGAGCCAACGCCTCGCAAGGCCCCAAGTCCACCCCCACCGCCGAGTTTGCCGAGAGGACGGTGGCCGCCGTCCTCAAAAAGAGGCCGCCCCCTTGGTTCTCCTATGGCCGCTATTCCACCATTATGGCCATCTTGTACCATTTGCCGATCTCCCTCAAGGACTTTCTCATGGGTGCACTCTCTAAGAGTTCTGGGGATCACAACCAC

Protein sequence

MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH

Homology

[Close]

BLAST of MS010491 vs. NCBI nr
Match: XP_022132423.1 (uncharacterized protein LOC111005282 [Momordica charantia])

HSP 1 Score: 560.5 bits (1443), Expect = 8.5e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV
Sbjct: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
           QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA
Sbjct: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK
Sbjct: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH 284
           KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH
Sbjct: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH 283

[Close]

BLAST of MS010491 vs. NCBI nr
Match: XP_038881070.1 (short-chain dehydrogenase RED1-like [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 4.7e-130
Identity = 235/280 (83.93%), Postives = 261/280 (93.21%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LA AFA   C+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLALAFADQGCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTSLVIEKFGRIDILVNNAG+QCIGPI EVPLSAMQNAFNTNVFGTMRLIQA
Sbjct: 61  LSDKSVERLTSLVIEKFGRIDILVNNAGIQCIGPIAEVPLSAMQNAFNTNVFGTMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H+++DCLRME+KPFGIDVINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPLPWAGAYTATKAALHSVSDCLRMELKPFGIDVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIGSSA+AGFN+MPELKLFK FE A++ARANASQGPK+TPTAEFA++TVAAVLK
Sbjct: 181 PGAIKSNIGSSAIAGFNQMPELKLFKPFEAAMLARANASQGPKATPTAEFADKTVAAVLK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGD 281
           K+P PWFSYG YST MAI+Y+LP+SLKDF++ ALSK   D
Sbjct: 241 KKPSPWFSYGGYSTFMAIMYYLPLSLKDFIVEALSKRVSD 280

[Close]

BLAST of MS010491 vs. NCBI nr
Match: XP_023003169.1 (uncharacterized protein LOC111496864 [Cucurbita maxima] >XP_023003170.1 uncharacterized protein LOC111496864 [Cucurbita maxima])

HSP 1 Score: 469.5 bits (1207), Expect = 2.0e-128
Identity = 226/276 (81.88%), Postives = 260/276 (94.20%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LARAFA  DC+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLARAFADQDCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTS+VIEKFGR+DILVNNAGVQCIGPI EVPLS+MQNAFNTNVFGTMRLIQA
Sbjct: 61  LSDQSVERLTSIVIEKFGRVDILVNNAGVQCIGPIAEVPLSSMQNAFNTNVFGTMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHM SRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H I+DCLR+E+KPFGI+VINVV
Sbjct: 121 VVPHMVSRRKGKIVNLGSVTVLAPIPWAGAYTATKAAIHAISDCLRLELKPFGIEVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SAVAGFN++P+LKLFK +E A++ARANASQGPK+ PTAEFAE+TVAA+LK
Sbjct: 181 PGAIKSNIGNSAVAGFNQLPDLKLFKPYEAAMLARANASQGPKAAPTAEFAEKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSK 277
           K+P PWFSYG YST+MA++YHLP+S+KDF++ A+SK
Sbjct: 241 KKPSPWFSYGGYSTLMALMYHLPLSIKDFIVEAMSK 276

[Close]

BLAST of MS010491 vs. NCBI nr
Match: XP_022963395.1 (uncharacterized protein LOC111463610 [Cucurbita moschata])

HSP 1 Score: 468.4 bits (1204), Expect = 4.4e-128
Identity = 228/282 (80.85%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LARAFA  DC+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLARAFADQDCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTS+VIEKFGRIDILVNNAGVQCIGPI EVPLS+MQNAFNTNVFG MRLIQA
Sbjct: 61  LSDQSVERLTSIVIEKFGRIDILVNNAGVQCIGPIAEVPLSSMQNAFNTNVFGAMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H I+DCLR+E+KPFGI+VINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPFPWAGAYTATKAAIHAISDCLRLELKPFGIEVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SAVAGFN+MPELKLFK +E A++ARANASQGP + PTAEFAE+TVAA+LK
Sbjct: 181 PGAIKSNIGNSAVAGFNQMPELKLFKPYEAAMLARANASQGPNAAPTAEFAEKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHN 283
           K+P PWFSYG YS +MA++YHLP+S+KDF++ A+SK + + N
Sbjct: 241 KKPSPWFSYGGYSNLMALMYHLPLSIKDFIVEAMSKRALNSN 282

[Close]

BLAST of MS010491 vs. NCBI nr
Match: XP_023517767.1 (uncharacterized protein LOC111781425 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 468.4 bits (1204), Expect = 4.4e-128
Identity = 227/276 (82.25%), Postives = 259/276 (93.84%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LARAFA  DC+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLARAFADQDCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTS+VIEKFGRIDILVNNAGVQCIGPI EVPLS+MQNAFNTNVFG MRLIQA
Sbjct: 61  LSDQSVERLTSIVIEKFGRIDILVNNAGVQCIGPIAEVPLSSMQNAFNTNVFGAMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H I+DCLR+E+KPFGI+VINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPFPWAGAYTATKAAIHAISDCLRLELKPFGIEVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SAVAGFN++PELKLFK +E A++ARANASQGP + PTAEFAE+TVAA+LK
Sbjct: 181 PGAIKSNIGNSAVAGFNQLPELKLFKPYEAAMLARANASQGPNAAPTAEFAEKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSK 277
           K+P PWFSYG YST+MA++YHLP+S+KDF++ A+SK
Sbjct: 241 KKPSPWFSYGGYSTLMALMYHLPLSIKDFIVEAMSK 276

[Close]

BLAST of MS010491 vs. ExPASy Swiss-Prot
Match: G4N286 (Short-chain dehydrogenase RED1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) OX=242507 GN=RED1 PE=2 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-30
Identity = 82/248 (33.06%), Postives = 135/248 (54.44%), Query Frame = 0


Query: 5   GKE-VVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSD 64
           GKE   LI+GC DGGIGHALA  F  +  +V+ T     S   LE   +  + +LDV  +
Sbjct: 4   GKEKFALITGCGDGGIGHALANNFVQNGFIVIATLLPHESRTHLEH-AKIHVIDLDVTKE 63

Query: 65  ESVERLTSLVIE-KFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVV 124
           + +    S + E   G +D+LVNNAG+       +  +  ++  F  NVFG MRL+  + 
Sbjct: 64  DQMIPFRSTLEEITGGTLDVLVNNAGICYTMTAADTDVKQVEKMFAVNVFGPMRLVHHLH 123

Query: 125 PHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPG 184
             + +  +G IVN+GS+  + P  +  +Y A+KAA+H   + LR+E+KPFG+ V+N++ G
Sbjct: 124 RMLIAAPRGVIVNIGSIGGVCPYVFGASYNATKAALHHWGNTLRVEMKPFGVHVVNIISG 183

Query: 185 AIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKR 244
            + +NI  S V     +PE  ++     A + + + ++ P +    ++A   VA V ++ 
Sbjct: 184 EVATNILKSDVRDNRTLPEDSVYAPL--AQLFKDHVNRTPDAMSPDDYARGVVAMVQRRS 243

Query: 245 PPPWFSYG 251
            P WF +G
Sbjct: 244 LPAWFWHG 248

[Close]

BLAST of MS010491 vs. ExPASy Swiss-Prot
Match: A0A140JWS5 (Short-chain dehydrogenase ptmH OS=Penicillium simplicissimum OX=69488 GN=ptmH PE=3 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.6e-27
Identity = 81/238 (34.03%), Postives = 122/238 (51.26%), Query Frame = 0


Query: 6   KEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDES 65
           +  VLI+GCS GGIG ALA AF      V  T+R    M  L       L  LDV  +  
Sbjct: 3   RRTVLITGCSQGGIGSALAEAFHQRGFHVFATARKTEKMTHLRDLDWMTLIPLDVTQESQ 62

Query: 66  VERLTSLVIEKF-GRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPH 125
           +     L+ +   G +D LVNNAG   I P+ +      +  F  N +G +R+IQ   P 
Sbjct: 63  ISAAVELIQKHTGGTLDYLVNNAGDGYIIPVLDCDQVHGRQIFEVNFWGPLRMIQEFSPL 122

Query: 126 MASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAI 185
           + + R G IVN+ SV       W G Y++SKAA+  +++ LR+E+KPFG+ V++V+ GA+
Sbjct: 123 LIAAR-GTIVNINSVASETLPLWLGIYSSSKAALLALSETLRLELKPFGVQVLSVMTGAV 182

Query: 186 KSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKR 243
           ++ I  +       +P    + A+E  I A+A  S+         +AER V  +L +R
Sbjct: 183 QTMIFQTNY----RLPPDSAYVAWEKQIAAQAEGSEQASRMSATVYAERVVGDILNRR 235

[Close]

BLAST of MS010491 vs. ExPASy Swiss-Prot
Match: A0A0E3D8L9 (Short-chain dehydrogenase PC-15 OS=Penicillium crustosum OX=36656 GN=PC-15 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.6e-27
Identity = 79/238 (33.19%), Postives = 121/238 (50.84%), Query Frame = 0


Query: 6   KEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDES 65
           +  VLI+GCS GGIG ALA  F      V  T+R    M  L    R  L  LDV  +  
Sbjct: 3   RRTVLITGCSQGGIGSALAEVFHQRGFHVFATARKTEKMKHLRDLDRMTLIPLDVTQESQ 62

Query: 66  VERLTSLVIEKF-GRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPH 125
           +     L+ +   G +D LVNNAG   I P+ +      +  F  N +G +R+IQ   P 
Sbjct: 63  ISAAVELIQKHTGGTLDYLVNNAGDGYIIPVLDCDQLHGRQIFEVNFWGPLRMIQEFSPL 122

Query: 126 MASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAI 185
           + + R G IVN+ SV       W G Y++SKAA+  +++ LR+E+KPFG+ V++V+ GA+
Sbjct: 123 LIAAR-GTIVNINSVASETLPLWLGIYSSSKAALLALSETLRLELKPFGVQVLSVMTGAV 182

Query: 186 KSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKR 243
           ++ I  +       +P    +  +E  I A+A  S+         +AER V  +L ++
Sbjct: 183 QTMIFQTNY----RLPPDSAYMTWEKQIAAQAEGSEKASRMSATVYAERVVGDILNRQ 235

[Close]

BLAST of MS010491 vs. ExPASy Swiss-Prot
Match: A0A0U1LQE2 (Short-chain dehydrogenase cctT OS=Talaromyces islandicus OX=28573 GN=cctT PE=3 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 1.1e-23
Identity = 70/237 (29.54%), Postives = 119/237 (50.21%), Query Frame = 0


Query: 7   EVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDESV 66
           + VLI+GCS GG+G A+A+ +      V  T R++  +  L       + EL+V S ES+
Sbjct: 3   KTVLITGCSHGGLGAAMAKIYHAKGFQVFATVRNKAKVGSLGGIDGIEIMELEVTSVESI 62

Query: 67  ERLTSLVIEKF-GRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPHM 126
            +  + V ++  G +DILVNNAGV  I P+ +  L   +  ++ NV+  + + QA  P M
Sbjct: 63  RQCANTVAKRTGGTLDILVNNAGVNAIVPLLDASLDDAKKVYDANVWSVVAMAQAFAP-M 122

Query: 127 ASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAIK 186
             + KG + N+ SV+      WAG Y++S++A   I++ LR+E+ P G+ V+ V+ G ++
Sbjct: 123 LIKAKGTMCNISSVSGEMVFAWAGVYSSSRSAGTRISETLRLELAPLGVRVVTVILGGVQ 182

Query: 187 SNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKR 243
           ++    +     E+P    ++     I         P        AE  V  +L  R
Sbjct: 183 TSGNDPSNIADLELPSSSYYQKITAVIDLHKKTMVHPNKQNVDVAAENVVNDLLNGR 238

[Close]

BLAST of MS010491 vs. ExPASy Swiss-Prot
Match: Q09851 (NADPH-dependent 1-acyldihydroxyacetone phosphate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ayr1 PE=3 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 2.3e-23
Identity = 75/231 (32.47%), Postives = 121/231 (52.38%), Query Frame = 0


Query: 6   KEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDES 65
           ++ VLI+GCS+GGIG+ALA  F      V+ T+R    M  L +     L +LDV  ++S
Sbjct: 4   EKFVLITGCSEGGIGNALALKFHQEGFQVLATARQVERMDNLTKAGLQTL-KLDVTDEDS 63

Query: 66  VERLTSLVIEKF--GRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVP 125
           V R     + KF  G +  L+NNAG  C  P  ++ +  +    + N +G +R+ +A   
Sbjct: 64  V-REVEQEVRKFTNGSLHYLINNAGAPCSAPAIDLDIEDVSKVMDVNFYGVIRMNKA-FQ 123

Query: 126 HMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGA 185
           H   R KG IVN+ S+    P  +  AY ASKAA+   ++ LR+E+ PFG+ V +++ G 
Sbjct: 124 HQLIRAKGTIVNVNSLVSYVPFAFNAAYNASKAALLAYSNTLRIELAPFGVQVTSIMTGG 183

Query: 186 IKSNIGSSAVAGFNE--MPELKLFKAFEGAIVARANASQGPKSTPTAEFAE 233
           +++ I S  +    E  +PE  ++  +   I+   N  +  K     EFA+
Sbjct: 184 VQTKIQSKPLGTMTEAAIPENSIYYPYRKLILENRNPVE--KFVTIEEFAD 229

[Close]

BLAST of MS010491 vs. ExPASy TrEMBL
Match: A0A6J1BW73 (uncharacterized protein LOC111005282 OS=Momordica charantia OX=3673 GN=LOC111005282 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.1e-156
Identity = 283/283 (100.00%), Postives = 283/283 (100.00%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV
Sbjct: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
           QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA
Sbjct: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK
Sbjct: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH 284
           KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH
Sbjct: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHNH 283

[Close]

BLAST of MS010491 vs. ExPASy TrEMBL
Match: A0A6J1KSI9 (uncharacterized protein LOC111496864 OS=Cucurbita maxima OX=3661 GN=LOC111496864 PE=3 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 9.6e-129
Identity = 226/276 (81.88%), Postives = 260/276 (94.20%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LARAFA  DC+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLARAFADQDCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTS+VIEKFGR+DILVNNAGVQCIGPI EVPLS+MQNAFNTNVFGTMRLIQA
Sbjct: 61  LSDQSVERLTSIVIEKFGRVDILVNNAGVQCIGPIAEVPLSSMQNAFNTNVFGTMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHM SRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H I+DCLR+E+KPFGI+VINVV
Sbjct: 121 VVPHMVSRRKGKIVNLGSVTVLAPIPWAGAYTATKAAIHAISDCLRLELKPFGIEVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SAVAGFN++P+LKLFK +E A++ARANASQGPK+ PTAEFAE+TVAA+LK
Sbjct: 181 PGAIKSNIGNSAVAGFNQLPDLKLFKPYEAAMLARANASQGPKAAPTAEFAEKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSK 277
           K+P PWFSYG YST+MA++YHLP+S+KDF++ A+SK
Sbjct: 241 KKPSPWFSYGGYSTLMALMYHLPLSIKDFIVEAMSK 276

[Close]

BLAST of MS010491 vs. ExPASy TrEMBL
Match: A0A6J1HHW0 (uncharacterized protein LOC111463610 OS=Cucurbita moschata OX=3662 GN=LOC111463610 PE=3 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.1e-128
Identity = 228/282 (80.85%), Postives = 261/282 (92.55%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LARAFA  DC+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLARAFADQDCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTS+VIEKFGRIDILVNNAGVQCIGPI EVPLS+MQNAFNTNVFG MRLIQA
Sbjct: 61  LSDQSVERLTSIVIEKFGRIDILVNNAGVQCIGPIAEVPLSSMQNAFNTNVFGAMRLIQA 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTA+KAA+H I+DCLR+E+KPFGI+VINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPFPWAGAYTATKAAIHAISDCLRLELKPFGIEVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SAVAGFN+MPELKLFK +E A++ARANASQGP + PTAEFAE+TVAA+LK
Sbjct: 181 PGAIKSNIGNSAVAGFNQMPELKLFKPYEAAMLARANASQGPNAAPTAEFAEKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSKSSGDHN 283
           K+P PWFSYG YS +MA++YHLP+S+KDF++ A+SK + + N
Sbjct: 241 KKPSPWFSYGGYSNLMALMYHLPLSIKDFIVEAMSKRALNSN 282

[Close]

BLAST of MS010491 vs. ExPASy TrEMBL
Match: A0A5A7SYA8 (Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002560 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.8e-127
Identity = 231/276 (83.70%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LA AFA   C+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLALAFADQGCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTSLVIEKFGRIDILVNNAGVQCIGPI EV LSAMQNAFNTNVFGTMRLIQ 
Sbjct: 61  LSDQSVERLTSLVIEKFGRIDILVNNAGVQCIGPIAEVHLSAMQNAFNTNVFGTMRLIQT 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTASKAA+H+I+DCLRME+KPFGIDVINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPLPWAGAYTASKAAIHSISDCLRMELKPFGIDVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SA+AGFN+MPELKLFK FE AI+ARANASQGPK+TPTAEFA++TVAA+LK
Sbjct: 181 PGAIKSNIGNSAIAGFNQMPELKLFKPFEAAILARANASQGPKATPTAEFAKKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSK 277
            +P PWFSYG YST+MA++YHLPI LKD ++  LSK
Sbjct: 241 TKPSPWFSYGGYSTLMAVMYHLPIFLKDLIVEFLSK 276

[Close]

BLAST of MS010491 vs. ExPASy TrEMBL
Match: A0A1S3B2C4 (uncharacterized oxidoreductase SSP0419 OS=Cucumis melo OX=3656 GN=LOC103485000 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 1.8e-127
Identity = 231/276 (83.70%), Postives = 255/276 (92.39%), Query Frame = 0


Query: 1   MEFAGKEVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDV 60
           MEFAGKEVV+ISGCSDGGIGH+LA AFA   C+VV TSRSR SMA+LE DPRFFLQELDV
Sbjct: 1   MEFAGKEVVVISGCSDGGIGHSLALAFADQGCLVVATSRSRSSMADLEHDPRFFLQELDV 60

Query: 61  QSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQA 120
            SD+SVERLTSLVIEKFGRIDILVNNAGVQCIGPI EV LSAMQNAFNTNVFGTMRLIQ 
Sbjct: 61  LSDQSVERLTSLVIEKFGRIDILVNNAGVQCIGPIAEVHLSAMQNAFNTNVFGTMRLIQT 120

Query: 121 VVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVV 180
           VVPHMASRRKGKIVNLGSVTV+AP PWAGAYTASKAA+H+I+DCLRME+KPFGIDVINVV
Sbjct: 121 VVPHMASRRKGKIVNLGSVTVLAPLPWAGAYTASKAAIHSISDCLRMELKPFGIDVINVV 180

Query: 181 PGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLK 240
           PGAIKSNIG+SA+AGFN+MPELKLFK FE AI+ARANASQGPK+TPTAEFA++TVAA+LK
Sbjct: 181 PGAIKSNIGNSAIAGFNQMPELKLFKPFEAAILARANASQGPKATPTAEFAKKTVAAILK 240

Query: 241 KRPPPWFSYGRYSTIMAILYHLPISLKDFLMGALSK 277
            +P PWFSYG YST+MA++YHLPI LKD ++  LSK
Sbjct: 241 TKPSPWFSYGGYSTLMAVMYHLPIFLKDLIVEFLSK 276

[Close]

BLAST of MS010491 vs. TAIR 10
Match: AT5G10050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 367.1 bits (941), Expect = 1.3e-101
Identity = 177/262 (67.56%), Postives = 216/262 (82.44%), Query Frame = 0


Query: 8   VVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDESVE 67
           VVLI+GCS GGIGHALAR F    C VV TSRSR +M +LEQD R F++ELDVQSD++V 
Sbjct: 9   VVLITGCSQGGIGHALAREFTEKGCRVVATSRSRSTMTDLEQDSRLFVKELDVQSDQNVS 68

Query: 68  RLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPHMAS 127
           ++ S VI+KFG+ID+LVNNAGVQC+GP+ E P+SAM+N FNTNVFG+MR+ QAVVPHM S
Sbjct: 69  KVLSEVIDKFGKIDVLVNNAGVQCVGPLAETPISAMENTFNTNVFGSMRMTQAVVPHMVS 128

Query: 128 RRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAIKSN 187
           ++KGKIVN+GS+TVMAP PWAG YTA+KAA+H +TD LR+E++PFGIDVINVVPG I++N
Sbjct: 129 KKKGKIVNVGSITVMAPGPWAGVYTATKAAIHALTDTLRLELRPFGIDVINVVPGGIRTN 188

Query: 188 IGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKRPPPWF 247
           I +SAVA FN+MPELKL+K +E AI  RA  SQ    TP   FA  TVAAVLKK PP WF
Sbjct: 189 IANSAVATFNKMPELKLYKPYEEAIRERAFISQRMNPTPAETFARDTVAAVLKKNPPAWF 248

Query: 248 SYGRYSTIMAILYHLPISLKDF 270
           S GRYST+MA++YH+P+ LKDF
Sbjct: 249 SSGRYSTLMAVMYHMPLWLKDF 270

[Close]

BLAST of MS010491 vs. TAIR 10
Match: AT5G65205.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.4e-100
Identity = 172/264 (65.15%), Postives = 219/264 (82.95%), Query Frame = 0


Query: 8   VVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQSDESVE 67
           VVLI+GCS GGIGHALAR F+ + C VV TSRS+++M ELE+D +FF+QELDVQS++SV 
Sbjct: 10  VVLITGCSQGGIGHALAREFSANGCRVVATSRSQKTMTELEKDSKFFVQELDVQSEQSVS 69

Query: 68  RLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVPHMAS 127
           ++ S VI+KFG+ID+LVNNAGVQCIGP+ E+P+SAM   FNTNV G+MR+ QAVVPHMAS
Sbjct: 70  KVVSKVIDKFGQIDVLVNNAGVQCIGPLAEIPISAMDYTFNTNVLGSMRMTQAVVPHMAS 129

Query: 128 RRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGAIKSN 187
           ++KGKIVN+GS+++MAP PWAG YTASKAA+H +TD LR+E+KPFGIDVIN+VPG I+SN
Sbjct: 130 KKKGKIVNIGSISIMAPGPWAGVYTASKAALHALTDTLRLELKPFGIDVINIVPGGIQSN 189

Query: 188 IGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAEFAERTVAAVLKKRPPPWF 247
           I +S ++ FN +PELKL+K FE AI  RA  SQ  K  PT  FA+ TV+ VLKK PP W+
Sbjct: 190 IANSGISSFNNLPELKLYKPFEDAIRERAFLSQNIKPIPTETFAKETVSVVLKKNPPAWY 249

Query: 248 SYGRYSTIMAILYHLPISLKDFLM 272
           S GR ST+MAI++H+PI +KDFL+
Sbjct: 250 STGRLSTVMAIMHHIPIFMKDFLL 273

[Close]

BLAST of MS010491 vs. TAIR 10
Match: AT1G10310.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 3.7e-16
Identity = 62/188 (32.98%), Postives = 95/188 (50.53%), Query Frame = 0


Query: 9   VLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSM----AELEQDPRFFLQELDVQSDE 68
           VLI+G S  G+G ALA   A     V+  +RS+  +    +EL       L   DV+S+ 
Sbjct: 20  VLITGVSK-GLGRALALELAKRGHTVIGCARSQEKLTALQSELSSSTNHLLLTADVKSNS 79

Query: 69  SVERLTSLVIEKFGRIDILVNNAG-VQCIGPIPEVPLSAMQNAFNTNVFGTMRLIQAVVP 128
           SVE +   ++EK G  DI+VNNAG +     I EV      N  +TNV G   +++  +P
Sbjct: 80  SVEEMAHTIVEKKGVPDIIVNNAGTINKNSKIWEVSAEDFDNVMDTNVKGVANVLRHFIP 139

Query: 129 HMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGIDVINVVPGA 188
            M  R++G IVN+ S    +       Y ASK A+  ++  +  EV   G+ V+ + PG 
Sbjct: 140 LMLPRKQGIIVNMSSGWGRSGAALVAPYCASKWAIEGLSRAVAKEVVE-GMAVVALNPGV 199

Query: 189 IKSNIGSS 192
           I + + +S
Sbjct: 200 INTELLTS 205

[Close]

BLAST of MS010491 vs. TAIR 10
Match: AT3G03330.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 80.5 bits (197), Expect = 2.4e-15
Identity = 73/277 (26.35%), Postives = 123/277 (44.40%), Query Frame = 0


Query: 7   EVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRFFLQELDVQ----- 66
           +VV I+G S  GIG  LA+ FA  D  ++ ++R++  +  ++ + +      DV+     
Sbjct: 44  KVVWITGAS-RGIGEILAKQFASLDAKLILSARNKAELDRVKSELKGKFAPEDVKVLPLD 103

Query: 67  ---SDESVERLTSLVIEKF--GRIDILVNNAGVQ-CIGPIPEVPLSAMQNAFNTNVFGTM 126
               +ES++ +    +  F    +D LV+NA  +       +     ++  F+ NVFGT+
Sbjct: 104 LASGEESLKHVVEQAVSLFPGAGVDYLVHNAAFERPKSKASDASEETLKTTFDVNVFGTI 163

Query: 127 RLIQAVVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGID 186
            L + V PHM  +  G  V + S     P+P    Y+ASK A+H     LR E    GI 
Sbjct: 164 SLTKLVAPHMLKQGGGHFVVISSAAGKVPSPGQAIYSASKHALHGYFHSLRSEFCQKGIK 223

Query: 187 VINVVPGAIKSNIGSSAVAGFNEMPELKLFKAFEGAIVARANASQGPKSTPTAE-FAERT 246
           V  V PG I++                     + G   + +  S+ P+   ++E  AE T
Sbjct: 224 VTVVCPGPIET---------------------WNGTGTSTSEDSKSPEKRVSSERCAELT 283

Query: 247 VAAVLKKRPPPWFSYGRYSTIMAILYHLPISLKDFLM 272
           + A        W SY     +M ++ ++P SL  +LM
Sbjct: 284 IIAASHNLKEAWISYQPVLLVMYLVQYMP-SLGFWLM 297

[Close]

BLAST of MS010491 vs. TAIR 10
Match: AT3G05260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 7.8e-14
Identity = 55/190 (28.95%), Postives = 88/190 (46.32%), Query Frame = 0


Query: 7   EVVLISGCSDGGIGHALARAFAGHDCVVVPTSRSRRSMAELEQDPRF------------F 66
           +V L++G  D GIG A+   +A     V  T    R   + E+  R              
Sbjct: 40  KVALVTG-GDSGIGKAVCHCYALEGASVAFTYVKGREDKDAEETLRLLHEVKTREAKEPI 99

Query: 67  LQELDVQSDESVERLTSLVIEKFGRIDILVNNAGVQCIGPIPEVPLSAMQNAFNTNVFGT 126
           +   D+  +E+ +R+   V+  FGRID+LVN A  Q    I ++  + ++  F TN+F  
Sbjct: 100 MIATDLGFEENCKRVVEEVVNSFGRIDVLVNCAAEQHEVSIEDIDEARLERVFRTNIFSQ 159

Query: 127 MRLIQAVVPHMASRRKGKIVNLGSVTVMAPTPWAGAYTASKAAVHTITDCLRMEVKPFGI 185
             L++  + HM  +    I+N  SV   A       YTA+K A+ + T  L +++ P GI
Sbjct: 160 FFLVKYALKHM--KEGSSIINTTSVVAYAGNSSLLEYTATKGAIVSFTRGLALQLAPKGI 219

The following BLAST results are available for this feature:

BLAST of MS010491 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022132423.1	8.5e-156	100.00	uncharacterized protein LOC111005282 [Momordica charantia]	[more]
XP_038881070.1	4.7e-130	83.93	short-chain dehydrogenase RED1-like [Benincasa hispida]	[more]
XP_023003169.1	2.0e-128	81.88	uncharacterized protein LOC111496864 [Cucurbita maxima] >XP_023003170.1 uncharac...	[more]
XP_022963395.1	4.4e-128	80.85	uncharacterized protein LOC111463610 [Cucurbita moschata]	[more]
XP_023517767.1	4.4e-128	82.25	uncharacterized protein LOC111781425 [Cucurbita pepo subsp. pepo]	[more]

BLAST of MS010491 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G4N286	1.2e-30	33.06	Short-chain dehydrogenase RED1 OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-46...	[more]
A0A140JWS5	1.6e-27	34.03	Short-chain dehydrogenase ptmH OS=Penicillium simplicissimum OX=69488 GN=ptmH PE...	[more]
A0A0E3D8L9	4.6e-27	33.19	Short-chain dehydrogenase PC-15 OS=Penicillium crustosum OX=36656 GN=PC-15 PE=3 ...	[more]
A0A0U1LQE2	1.1e-23	29.54	Short-chain dehydrogenase cctT OS=Talaromyces islandicus OX=28573 GN=cctT PE=3 S...	[more]
Q09851	2.3e-23	32.47	NADPH-dependent 1-acyldihydroxyacetone phosphate reductase OS=Schizosaccharomyce...	[more]

BLAST of MS010491 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BW73	4.1e-156	100.00	uncharacterized protein LOC111005282 OS=Momordica charantia OX=3673 GN=LOC111005...	[more]
A0A6J1KSI9	9.6e-129	81.88	uncharacterized protein LOC111496864 OS=Cucurbita maxima OX=3661 GN=LOC111496864...	[more]
A0A6J1HHW0	2.1e-128	80.85	uncharacterized protein LOC111463610 OS=Cucurbita moschata OX=3662 GN=LOC1114636...	[more]
A0A5A7SYA8	1.8e-127	83.70	Putative oxidoreductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3B2C4	1.8e-127	83.70	uncharacterized oxidoreductase SSP0419 OS=Cucumis melo OX=3656 GN=LOC103485000 P...	[more]

BLAST of MS010491 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10050.1	1.3e-101	67.56	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G65205.1	1.4e-100	65.15	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G10310.1	3.7e-16	32.98	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G03330.1	2.4e-15	26.35	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G05260.1	7.8e-14	28.95	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 78..89 score: 59.74 coord: 151..170 score: 35.77 coord: 131..139 score: 54.33
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 125..141 score: 30.67 coord: 151..170 score: 33.96 coord: 78..89 score: 64.13 coord: 172..189 score: 31.02 coord: 9..26 score: 31.92
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 8..191 e-value: 2.7E-46 score: 157.5
None	No IPR available	GENE3D	3.40.50.720		coord: 6..260 e-value: 5.3E-58 score: 198.4
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 10..243 e-value: 3.1E-36 score: 122.8
None	No IPR available	PANTHER	PTHR44169:SF12	BNAC02G00550D PROTEIN	coord: 7..271
None	No IPR available	PANTHER	PTHR44169	NADPH-DEPENDENT 1-ACYLDIHYDROXYACETONE PHOSPHATE REDUCTASE	coord: 7..271
None	No IPR available	CDD	cd05374	17beta-HSD-like_SDR_c	coord: 8..253 e-value: 1.56332E-82 score: 246.759
IPR020904	Short-chain dehydrogenase/reductase, conserved site	PROSITE	PS00061	ADH_SHORT	coord: 138..166
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 7..257

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010491.1	MS010491.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity