MS010482 (gene) Bitter gourd (TR) v1

Overview


Name	MS010482
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	protein NEN4-like
Location	scaffold35: 3440 .. 4670 (-)
RNA-Seq Expression	MS010482
Synteny	MS010482

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAGAATACCCAATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCGACGGATTTGTCAGCCGTCGCTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCGGACAAGATATTCGGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGGTGAGAAAACAACATTAGAATTAGAATCATGAAAAAGAAAATCATAACATCCAGAATAGAATCCTCATATTTTGAACTGTTTCATGTTTAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGTACATAATATTAGATCAACTCCCTCCCTCTGCCTTTTTGTTATAATATCACTTTGGTTCACAAAAGTTCCTTTTGCAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGTATGTGCACAAAAGTTTCGACCATTTATGCAGCTCTTCTTGCATACCTGAGTTATGTAAATGGAATTTCTCTTTGTGGGTCACCTCTGATTTCGCTAATTTCTCTACCAGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGGTTCAGTTACAGGTCTTTCTTTAAATGTCAATGATATTTCATATATACATATATGGTGAAATTTTTTTCCAATCAACTGATGAAAACCTGCTTATTTGAAACAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCAAACATTCTCGTTCGCTGCTACGA

mRNA sequence

CCAGAATACCCAATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCGACGGATTTGTCAGCCGTCGCTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCGGACAAGATATTCGGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCAAACATTCTCGTTCGCTGCTACGA

Coding sequence (CDS)

CCAGAATACCCAATGGAGGCTTCAAGTTCGTGCAAGGATGGTGCAGCCGAGATCGTCTTCTTCGACTTGGAAACCACGGTGCCGAATAGAGTGGGGCAGCGGTTTCGAGTATTGGAATTTGGTGCCATTGTTGTCTGCCCCCTTAAGCTCGTGGAGCTTGAGAGCTTCACCACCCTCATTAGACCGACGGATTTGTCAGCCGTCGCTTCGAGGTCCAGCCGACGTGACGGGATAACAAGAGAAACCGTTGCAACTGCGCCATTGTTTGAGGAAGTTGCGGACAAGATATTCGGCATCTTGAATGGAAGGATATGGGCAGGCCATAACATTCAAAGATTTGACTGTGTTCGTATCAGGGAGGCGTTTGCAGAGATTGGCAGGCCTGCTCCTACGCCAGTCGGGATGGTTGATTCTTTAGGAGTCTTAACTGCAAAGTTTGGCAAGAGAGCTGGAAATATGAAGATGGCATCATTGGCTTCTTACTTTCAGCTGGGGCAGCAAAAGCACAGGAGCCTCGACGATGTTCGTATGAATCTAGAGGTCCTCAAGCATTGTGCTACTGTTCTGTTTCTGGAATCGACCCTCCCAAGCATATTACACGGAAAGTGGAAAACTTCATCCACCACAACAACTCGAAACAGAGCCAACGGGAAGTTGCATTGCAGAGAAGAAAGTAGTCGAAAATCTCCTCCAACGTCACCAGGATATCAACTGAGATCAGTTCCTTATGCAAGAGAGAGATTGGGAATTGGAAAGATGACAGAAAAGGTAAAGAATGTACTCTGCAGAGCTCAGGGAAAACAGAATTTTGACATCTTACTCAAACATTCTCGTTCGCTGCTACGA

Protein sequence

PEYPMEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYARERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLLR

Homology

[Close]

BLAST of MS010482 vs. NCBI nr
Match: XP_022133010.1 (protein NEN4 isoform X2 [Momordica charantia])

HSP 1 Score: 544.7 bits (1402), Expect = 4.8e-151
Identity = 275/278 (98.92%), Postives = 276/278 (99.28%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAV SRSSRRDGITRETVATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 245 RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           RERLGIGKMTEKVKNVLCRAQGKQNFDILL+HSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of MS010482 vs. NCBI nr
Match: XP_022133009.1 (protein NEN4 isoform X1 [Momordica charantia])

HSP 1 Score: 538.9 bits (1387), Expect = 2.6e-149
Identity = 275/282 (97.52%), Postives = 276/282 (97.87%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAV SRSSRRDGITRETVATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 245 RERLGIGK----MTEKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           RERLGIGK    MTEKVKNVLCRAQGKQNFDILL+HSRSLLR
Sbjct: 241 RERLGIGKVQLQMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 282

[Close]

BLAST of MS010482 vs. NCBI nr
Match: XP_023518830.1 (protein NEN4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.1 bits (1289), Expect = 6.1e-138
Identity = 259/278 (93.17%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSS K+G AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA+RSSRRDGITRE VATAPLFEEV+DKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVSDKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 245 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLL 282
            RERLGI KMT KVKNV+CRAQGKQ F+ILLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MS010482 vs. NCBI nr
Match: XP_022962945.1 (protein NEN4-like [Cucurbita moschata])

HSP 1 Score: 501.1 bits (1289), Expect = 6.1e-138
Identity = 259/278 (93.17%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSS K+G AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA+RSSRRDGITRE VATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 245 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLL 282
            RERLGI KMT KVKNV+CRAQGKQ F+ILLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MS010482 vs. NCBI nr
Match: KAG7026830.1 (Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 499.6 bits (1285), Expect = 1.8e-137
Identity = 258/278 (92.81%), Postives = 264/278 (94.96%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSS K+G AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA+RSSRRDGITRE VATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAF E
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFVE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILEGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 245 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLL 282
            RERLGI KMT KVKNV+CRAQGKQ F+ILLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MS010482 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.0e-91
Identity = 177/268 (66.04%), Postives = 204/268 (76.12%), Query Frame = 0


Query: 17  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRRD 76
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V+ RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 77  GITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMV 136
           GITR  V  AP FE+VA+KI G+LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 137 DSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196
           DSLG+L+ KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 197 SILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQL--RSVPYARERLGIGKMT 256
           + L GKW TSS   TR+R N                   YQ+  R++PY++  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 257 EKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           + VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of MS010482 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-70
Identity = 145/271 (53.51%), Postives = 180/271 (66.42%), Query Frame = 0


Query: 7   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 66
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIG 126
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 187 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 246
           TVLFLES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 247 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 273
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 266

[Close]

BLAST of MS010482 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 2.0e-67
Identity = 129/199 (64.82%), Postives = 156/199 (78.39%), Query Frame = 0


Query: 16  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRR 75
           +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++S S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 76  DGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 135
           +GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P P G 
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 136 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 195
           +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

Query: 196 PSILHGKWKTSSTTTTRNR 215
           P        +  T ++R R
Sbjct: 187 PYAHVDNSVSPETISSRRR 204

[Close]

BLAST of MS010482 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.2e-65
Identity = 126/219 (57.53%), Postives = 164/219 (74.89%), Query Frame = 0


Query: 16  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRR 75
           +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +  RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 76  DGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 135
           +GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P P G 
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 136 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 195
           +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

Query: 196 PSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSP 235
           P  L     T++T  T +R       R  + +KSP  SP
Sbjct: 194 PDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of MS010482 vs. ExPASy TrEMBL
Match: A0A6J1BU43 (protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 2.3e-151
Identity = 275/278 (98.92%), Postives = 276/278 (99.28%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAV SRSSRRDGITRETVATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 245 RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           RERLGIGKMTEKVKNVLCRAQGKQNFDILL+HSRSLLR
Sbjct: 241 RERLGIGKMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 278

[Close]

BLAST of MS010482 vs. ExPASy TrEMBL
Match: A0A6J1BUQ8 (protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.3e-149
Identity = 275/282 (97.52%), Postives = 276/282 (97.87%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAV SRSSRRDGITRETVATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVTSRSSRRDGITRETVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 240

Query: 245 RERLGIGK----MTEKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           RERLGIGK    MTEKVKNVLCRAQGKQNFDILL+HSRSLLR
Sbjct: 241 RERLGIGKVQLQMTEKVKNVLCRAQGKQNFDILLEHSRSLLR 282

[Close]

BLAST of MS010482 vs. ExPASy TrEMBL
Match: A0A6J1HGK3 (protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 3.0e-138
Identity = 259/278 (93.17%), Postives = 265/278 (95.32%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSS K+G AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVEL+SFTTLIRPTD
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELDSFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA+RSSRRDGITRE VATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRRDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGRPAPMPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQLRSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQLRSVPYA 240

Query: 245 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLL 282
            RERLGI KMT KVKNV+CRAQGKQ F+ILLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCRAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MS010482 vs. ExPASy TrEMBL
Match: A0A6J1KYA7 (protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.6e-136
Identity = 256/278 (92.09%), Postives = 262/278 (94.24%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEASSS K+G AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD
Sbjct: 1   MEASSSFKEG-AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 60

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA+RSSR DGITRE VATAPLFEEVADKIF ILNGRIWAGHNIQRFDCVRIREAFAE
Sbjct: 61  LSAVAARSSRHDGITREIVATAPLFEEVADKIFSILNGRIWAGHNIQRFDCVRIREAFAE 120

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IG+PAP PVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 121 IGKPAPNPVGMVDSLGVLTQKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 180

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL GKWKTSST TTRNRANGKLHCREE SRKSPP+SPGYQ RSVPYA
Sbjct: 181 CATVLFLESTLPSILQGKWKTSSTITTRNRANGKLHCREEGSRKSPPSSPGYQARSVPYA 240

Query: 245 -RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLL 282
            RERLGI KMT KVKNV+C AQGKQ F+ILLKHSRSLL
Sbjct: 241 TRERLGIAKMTAKVKNVVCSAQGKQPFNILLKHSRSLL 277

[Close]

BLAST of MS010482 vs. ExPASy TrEMBL
Match: A0A5D3CL58 (Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002650 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.1e-135
Identity = 249/278 (89.57%), Postives = 260/278 (93.53%), Query Frame = 0


Query: 5   MEASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTD 64
           MEAS S K+GA EIVFFDLETTVP RVGQRFRVLEFGAIVVCP+KLVELESF TLI+PTD
Sbjct: 5   MEASCSFKEGATEIVFFDLETTVPKRVGQRFRVLEFGAIVVCPMKLVELESFATLIKPTD 64

Query: 65  LSAVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAE 124
           LSAVA RSSR DGITRE VATAPLFE+VADKIF ILNGRIWAGHNI+RFDCVRI+EAFAE
Sbjct: 65  LSAVALRSSRPDGITREAVATAPLFEDVADKIFSILNGRIWAGHNIRRFDCVRIKEAFAE 124

Query: 125 IGRPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184
           IGRPAPTPVGMVDSLGVLT KFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH
Sbjct: 125 IGRPAPTPVGMVDSLGVLTNKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKH 184

Query: 185 CATVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQLRSVPYA 244
           CATVLFLESTLPSIL  KWKTS T TTRNRANGKLHCREESSRKSPP+SPGYQLRSVPY+
Sbjct: 185 CATVLFLESTLPSILQDKWKTSPTKTTRNRANGKLHCREESSRKSPPSSPGYQLRSVPYS 244

Query: 245 RERLGIGKMTEKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           RERLGIGKMT KVKNVL  AQGKQ+ ++LLKHSRSLLR
Sbjct: 245 RERLGIGKMTAKVKNVLYMAQGKQHLNVLLKHSRSLLR 282

[Close]

BLAST of MS010482 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 337.0 bits (863), Expect = 1.4e-92
Identity = 177/268 (66.04%), Postives = 204/268 (76.12%), Query Frame = 0


Query: 17  EIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRRD 76
           EIVFFDLET VPN+ GQ F +LEFGAI+VCP KL ELESFTTLI+P DLS V+ RSSR D
Sbjct: 9   EIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRSD 68

Query: 77  GITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGMV 136
           GITR  V  AP FE+VA+KI G+LNGRIWAGHNI+RFDCVRI+EAFAEIG+ AP P G++
Sbjct: 69  GITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGII 128

Query: 137 DSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTLP 196
           DSLG+L+ KFGKRAGNMKMASLA+YF LG QKHRSLDDVRMNLEVLKHCATVLFLESTLP
Sbjct: 129 DSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTLP 188

Query: 197 SILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSPGYQL--RSVPYARERLGIGKMT 256
           + L GKW TSS   TR+R N                   YQ+  R++PY++  L   KMT
Sbjct: 189 NHLEGKWHTSSKIMTRSRRN-------------------YQIAQRAMPYSKGSL--EKMT 248

Query: 257 EKVKNVLCRAQGKQNFDILLKHSRSLLR 283
           + VKN+L +AQG Q    L+ HS SLLR
Sbjct: 249 QNVKNLLSKAQGNQTLQSLINHSHSLLR 255

[Close]

BLAST of MS010482 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.1e-71
Identity = 145/271 (53.51%), Postives = 180/271 (66.42%), Query Frame = 0


Query: 7   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 66
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIG 126
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLEV+K+CA
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCA 181

Query: 187 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 246
           TVLFLES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 TVLFLESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 247 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 273
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 266

[Close]

BLAST of MS010482 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.4e-68
Identity = 129/199 (64.82%), Postives = 156/199 (78.39%), Query Frame = 0


Query: 16  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRR 75
           +EI FFD+ETTVP R GQRF +LEFG+I+VCP KL EL S+TTL++P DLS ++S S R 
Sbjct: 7   SEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLSVRC 66

Query: 76  DGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 135
           +GI R+ V  APLF ++AD ++ IL+GRIWAGHNI RFDC RIREAFAEIGR  P P G 
Sbjct: 67  NGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEPKGA 126

Query: 136 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 195
           +DSLG+LT KFG+RAG+MKMA+LA YF LG Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 127 IDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLESSL 186

Query: 196 PSILHGKWKTSSTTTTRNR 215
           P        +  T ++R R
Sbjct: 187 PYAHVDNSVSPETISSRRR 204

[Close]

BLAST of MS010482 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 249.6 bits (636), Expect = 3.0e-66
Identity = 126/219 (57.53%), Postives = 164/219 (74.89%), Query Frame = 0


Query: 16  AEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLSAVASRSSRR 75
           +EI FFD+ETT+P RVGQ + +LEFG+I+VCP KLVEL++++ L+RP +L+ +  RS + 
Sbjct: 14  SEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITPRSVKC 73

Query: 76  DGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIGRPAPTPVGM 135
           +GI RE V +A  F ++AD ++ IL+GRIWAGHNI +FDC RIREAFAEIGR  P P G 
Sbjct: 74  NGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPPEPKGT 133

Query: 136 VDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCATVLFLESTL 195
           +DSL +LT +FG+RAG+MKMA+LA+YF LG Q HRSLDDVRMN EVLK+CATVLFLES+L
Sbjct: 134 IDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLFLESSL 193

Query: 196 PSILHGKWKTSSTTTTRNRANGKLHCREESSRKSPPTSP 235
           P  L     T++T  T +R       R  + +KSP  SP
Sbjct: 194 PDELIENSVTTTTPETSSR-------RRRTIKKSPLQSP 225

[Close]

BLAST of MS010482 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 240.7 bits (613), Expect = 1.4e-63
Identity = 136/271 (50.18%), Postives = 169/271 (62.36%), Query Frame = 0


Query: 7   ASSSCKDGAAEIVFFDLETTVPNRVGQRFRVLEFGAIVVCPLKLVELESFTTLIRPTDLS 66
           ASS   D  +EI FFDLET VP + G+ F +LEFGAI+VCP +L EL S++TL+RPTDLS
Sbjct: 2   ASSLGGDERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLS 61

Query: 67  AVASRSSRRDGITRETVATAPLFEEVADKIFGILNGRIWAGHNIQRFDCVRIREAFAEIG 126
            +++ + RR GITR+ V +A  F E+ADK++ IL+GRIWAGHNI RFDCVRIREAFAEIG
Sbjct: 62  LISTLTKRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIG 121

Query: 127 RPAPTPVGMVDSLGVLTAKFGKRAGNMKMASLASYFQLGQQKHRSLDDVRMNLEVLKHCA 186
              P P   +DSL +L+ KFGKRAG+MKMASLA+YF LG Q HRSLDDVRMNLE      
Sbjct: 122 LSPPEPKATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE------ 181

Query: 187 TVLFLESTLPSILHGKWKTSSTTTTRNRANGKLHCR-----EESSRKSPPTSPGYQLRSV 246
                ES++P IL      S   + R R+NGKL          SS  SP T P       
Sbjct: 182 -----ESSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDA 241

Query: 247 PYARERLGIGKMTEKVKNVLCRAQGKQNFDI 273
              +    +  +TE      C      ++DI
Sbjct: 242 TIVKNHPIVSLLTE------CSESDASSYDI 255

The following BLAST results are available for this feature:

BLAST of MS010482 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133010.1	4.8e-151	98.92	protein NEN4 isoform X2 [Momordica charantia]	[more]
XP_022133009.1	2.6e-149	97.52	protein NEN4 isoform X1 [Momordica charantia]	[more]
XP_023518830.1	6.1e-138	93.17	protein NEN4-like [Cucurbita pepo subsp. pepo]	[more]
XP_022962945.1	6.1e-138	93.17	protein NEN4-like [Cucurbita moschata]	[more]
KAG7026830.1	1.8e-137	92.81	Protein NEN4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS010482 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJ23	2.0e-91	66.04	Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1	[more]
Q9CA74	1.5e-70	53.51	Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1	[more]
Q9FLR0	2.0e-67	64.82	Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1	[more]
Q0V842	4.2e-65	57.53	Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1	[more]

BLAST of MS010482 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BU43	2.3e-151	98.92	protein NEN4 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1BUQ8	1.3e-149	97.52	protein NEN4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005707 PE=4 SV=1	[more]
A0A6J1HGK3	3.0e-138	93.17	protein NEN4-like OS=Cucurbita moschata OX=3662 GN=LOC111463299 PE=4 SV=1	[more]
A0A6J1KYA7	1.6e-136	92.09	protein NEN4 OS=Cucurbita maxima OX=3661 GN=LOC111497460 PE=4 SV=1	[more]
A0A5D3CL58	3.1e-135	89.57	Protein NEN4 isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of MS010482 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39810.1	1.4e-92	66.04	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.1	1.1e-71	53.51	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G07710.1	1.4e-68	64.82	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G61390.1	3.0e-66	57.53	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G74390.2	1.4e-63	50.18	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013520	Exonuclease, RNase T/DNA polymerase III	SMART	SM00479	exoiiiendus	coord: 17..191 e-value: 3.3E-30 score: 116.3
IPR013520	Exonuclease, RNase T/DNA polymerase III	PFAM	PF00929	RNase_T	coord: 19..182 e-value: 2.2E-28 score: 99.9
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 12..189 e-value: 3.2E-30 score: 107.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 203..238
None	No IPR available	PANTHER	PTHR30231	DNA POLYMERASE III SUBUNIT EPSILON	coord: 9..282
None	No IPR available	PANTHER	PTHR30231:SF26	PROTEIN NEN4	coord: 9..282
None	No IPR available	CDD	cd06127	DEDDh	coord: 19..183 e-value: 1.00672E-25 score: 97.7564
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 18..188

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010482.1	MS010482.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding