MS010425 (gene) Bitter gourd (TR) v1

Overview
NameMS010425
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionArp2/3 complex 34 kDa subunit
Locationscaffold878: 799474 .. 804237 (+)
RNA-Seq ExpressionMS010425
SyntenyMS010425
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCATTCTTTCAGAGAGCTTCACCAGCTTTGAAGGAAATCCTCCTCAAACTGTGCAGGTGAGAATCTATTTCATGTGTTTGAATATTTTAACAGATAATTTTGTGTTTGATTAAAACTTTGAGAGAATTTTAGATATTAAATTTGCATATGATTGTGCTGGAAAATAGCTATTTTGAAGGTTTCTTTTTTTCGGTTATGAGCACAGTTTGGAAAAGCCCACAGAAATTGAGCATCACTTGCATGAATTTGGTTCTGTTCAATACCTTATCCAGGTAGAATAAATCTCATTCATCTGGGATTGTGCACGTTTAACATTTGAAATAATGTCTTGATGGCTTTCTGTTGTTTGGCAGTTCTCAGTATCGGATCCACAGAATATTTACCTGTCGATTTCAACCCCATTGTTGTCACAAGGAGCTCTGTTGTCTGATGGGCTCTCATCGCACACTGTCGAGATGGTAAAACAAATTTGCTCTCATGTTGTTGAGATCGTTGAACCTGCAAGAGAAGGATACCAACTTACTCTAAAAATCGATTTTGCACAAATTTTGCATGGGAAAGGTATGATTATCCACTTTTAGTTTTCACACTTGAACAGCTTCATTAATTGAAAATTAATTGACGAAAAGAATGAGAGGTGTTGATACTGCATAGTTTCAGAAAACAAATCATTTTGCTACTTGACATTCTGTAGAAGGTTGAGTTTAGGCCCATACTCATCCTGTTGTTGAGCAAGAATCTTGATGATTATAGTATAAGATTGAAATATGGGCTTTTCTTTATAACTTCAACTGATGTGGATGTTCTTTTTGCAGAGTCGGAAAAGATAATTACAGAGATTGCTGCTGTCCAAGCAGTGATTCTAAGTTCTCAGCTGAAAGAAATGTTGAGAAATGTTAATTCACAGGCTCTATTCCATGAAACATGCAGACCCATCAAACTTTTATATCATCCAAGGGAACCATTCTTTGTTATCAAGCAGGCAAGTTGAGAATGTATATTATTTTATTTCATAATCAGAACTCTAAAGTTGAGTTTATATTAACTAAGGTTTCCAGTGGGTTTATTTTGTCTCGCTCTAATGTGCCAATTCATTATAAACCCCTTCCTATATAAACAGATGAATGTTGAGTGACAGATAAGAACTGAGAAATTTGGTTGGGTTGTTCCAAATTTTGAGGAGCGAGACTTCTGACATTTAAGTTCTACTCTTGACTCTTCTGTGCATTTTGACATCTCTAAATAGACGAAAATCTTGCATACAGGCCCAGAAGATTCTCGTAATTTTCCCAATCCGTTTCAAGGAAAGTACAGATGTGATCATTGCAACAGCCTTTTTCCGGGTATGTTGCTTAAATAGCAATGCAAGGATCTGTTCTTATTTTTTAAAAAACTATATGAATTTATTTGGATAATGCAACTATTGTAGTCAAAATAGGAACTTATGGATGTGGGAAGCTCCGAAAAATGGGCAAAAGCACCTCCCTGCTGTTGGTCACCCATACCCCCGCCAGAACTGAGAGGCGAACCTTTGGAAGAACTGAGCACCAATGGAGGATTTGTCACCTTCGGTAAGATATCCATTTCAGCAAATGTAGTCTAGAAAAACGTTAATTTCTTGAGAGTATTCTTTCATGTATATGTTTATGTATGCGTTACATACAAAGTAAAAGAATGGAATCCAACACCAGCTGTATTAAACAGATTTCTCATAGTAACAACGGAAACGGAATTTGTTCAACTTGTTGGATTTGTATTAGTAAGTTAGAAATTTTTTAATACTCTCAAACTCTGTTCGCATAATGCATCGACAGATATTTCTCTGCGTCACGTTGAAGGTAAAAGACTTGACAAAACTGTATGGAGTTTGCTGAACTTTAATGCATATGTGAAGTACCATGTAAAGGTATCATGTTTGTTTATTCTTTTTATCCAAAACCTCTGTATCTGTTGTGGACAATGTTTCCCCTATTCTTCTGGGAATAACTTTAAGCGTGTCTTTGGTGAACTTTCCTCACAAGACAACCAGAGGTTTTATCCAGAGAAGGATGAGAAAGCGGTTAGAAGGGCTTGTTGAGGTAAGTTTTAAGTATACAAATATTTGTAAAATCAAAAGGCAATTTATCAGTGATATTTGGAAGAAGAGATTTCTCTAAAATTAAAAAATAAATGTTGTTTCCCATAACAAATGTCACTATGCTATTTGATTAATTTTGTTTTCCCAAATTTGTAGATCCTATATCAGAAAGGTTCAGATGATGTCCTACTCAACAAGGGTCAAGGTATGCTCTTAACAAATATAATAATAATGTGAATTACCATTGCCTTCTCATAATTTTCTCTCTCGTTTTTTGCATCTGGATGAGTTTCAACTTGATCTTGAGTTGCTGGGTGAGAAACAAACAATGTAAAACCAACCATTATAACATGCAGAGAGATCTCTTAATACCCTGAGTGTGGGTGAAAATGAAAAACCAAAAACGGGCCAAATTGATATCGATCGATCGGAAGGTGTGCATAGTCTAATGTAGCTACCTTCATGTTGGAGTCAAATCTCTATTCTAATCGACTCCGACCGAATTTTACTCTAACTCTAATGTTGAGTGAGTTTTAAGTTTGGTGATCAAACCAACAATTTATCTCTCATAGCTTAATTCCGTGCAGATGCAGGCATTATATATATGAAGAAACTGGTAGCCAAAACTAAACACCTCAAACAGAAATGTCGTAATTTGAGCAAGAAGATCAAGAGATTCCGATTTCGAATTAAAATCCCTGGATTTGCACGGTTTCGTCGAAGATGGTTGAAGTTCCCCAAGTTTTCTTCTTCAATTGGATACACCAGACTAAAATGAAGTGATTTGACATCAATAGAAAAACTTTAGAAATAACATATGGTGTGTCTAGAGACAAAAATGATAGGTGGTAGAAGAAGAATTCTTGGTATTTGCATGAAGTCTTAGAGTTTAGTATCTATTGAAACGAAAAAGTTTCAACTTTTACACTTCCTATATATAATGTTTGCCTTTGATAATTTTCATAGAGAACCTTGTGTTTGAGATTTTTATTCATCCTCATCGTAGTTTATTTTGTTGAGTTTTTATATAGCTCAATTAGTTAAGATATATATTTTCGACCAGAAGGTCGGAGATTCAAATCCCCACATCTGTTTGAACTAAAAAAATTTTCATATGAAAAATAGATTGCCTTAAAAGAGGGAAAAAAATGTTTGAGTGTCTTTCTTTTATCAAGAATCACACATATAAGACTTTGCTCATTAGCTAATTTGATTGAGATTTTTCACAACAATTCCATTGATCAAATCGGTGATAAGATCGAAAAAAAATAAGGAGAGAATAGTTGTAAATCTTCAGCGTTGTTAAGAAAAATGTTATTTTCTGTCTCAAGTGGTTACCTAGAATTCCCCTACATGAGATAGGAAGATGGGTTTGCTATTTAATAGAGCAAAATTCTTGAAAACTGAAGTTTACTTTATAAGAAAATCAATGAAAATGTCATTTTTGACAGAGTAAAAGCATGTTAATACGAAAGCTACCCATGTTAAACTTTTAAAGTGGTTTGGTACGTGAAAATGCATCTATGAGATTGGAGAGTTCATAAGTTCAACAATTACAGGCTGGAGGTCGAATCACCAACATGTGTTAAACCTTTTTTCTTTTCAAAATACGATAATTCAAGTCGATATGAGCATAGCTTAACAGTAATTGACATGTACTCATTCCTTCGAAGTTGGAGGTTCGATCTCCCATGTCCCTAAATTGTAGTACTAAAAAAACGATAACTCAAAATAAAGTAGAGATTTAAACTTACGACTTATAAAGAATGTCTTGTTGAACTATTTTGATTCTAATTTAAACTATAGATTTTATCTGGATGGTTCTATGCTATTAAATACTTTCTCGGAAATAAGGTTTACTAAGCGGCTAGGAAAATGCTGCCTGTGATGAAATCAATTGGTAAGTAGACAGATGGAAGCAATCAGGAAAAAGAGCCAATTGAAGTCTGGATTCTGATAATTTTCCAAAACAATAGGGACCAGAAGTCACTTTAAACCAAAACCCAAGCCACCAATTTGTGTGGCTCAAAAAATGACTCCTCCACTCTCTCTCTCTCATCTCCATCCAATCTCTCCCATACTACCTCAAAAATATCCACCTTTTTCACATACCATACCAAATCCATCATCAATGCTTCCATTACAAGTTCCAGCATTGCTGGTAAACACTGCTCTCTGATCTTCCACACCCTTTCATGGCAACTTCAATTTCAGCAACTGGGCTTCTTCATAAACCACCTCTTTCCTCCTCCTTCCATGGAGGCTGGGGGACTTCGATCTGTGGCGACGACAGCGCCATGCTGATCAAGTCGGTCCCGGCACAGGTTCGAGTCACAAAGCCGGTGAGATTCCGGCCAATGATGAAGAATGTTAATGAAGGGAAGGGTCTGTTTGCGCCTGTGGTAGTTCTTGCTAGAAATATTATTGGAAAGAAACGTTTCAATCAGTTGAGAGGCAAGGCCATTGCCCTGCACTCACAGGTAATCACTTGAATTCTCTTATCCATACATTACAATTCACATACTGATCTTCTGATACAGGTTTAGAGATAAACTTGTGTTGAATTTTTTCCTTTTGGACAGATAATTACTGAGTTCTGCAAATCTATAGGAGCTGATGCAAAGCAAAGGCAGGGGTTGATTAGGTTAGCCAAGAAGAATGGAGAAAGATTAGGATTCCTTGCT

mRNA sequence

ATGGCATTCTTTCAGAGAGCTTCACCAGCTTTGAAGGAAATCCTCCTCAAACTGTGCAGTTTGGAAAAGCCCACAGAAATTGAGCATCACTTGCATGAATTTGGTTCTGTTCAATACCTTATCCAGTTCTCAGTATCGGATCCACAGAATATTTACCTGTCGATTTCAACCCCATTGTTGTCACAAGGAGCTCTGTTGTCTGATGGGCTCTCATCGCACACTGTCGAGATGGTAAAACAAATTTGCTCTCATGTTGTTGAGATCGTTGAACCTGCAAGAGAAGGATACCAACTTACTCTAAAAATCGATTTTGCACAAATTTTGCATGGGAAAGAGTCGGAAAAGATAATTACAGAGATTGCTGCTGTCCAAGCAGTGATTCTAAGTTCTCAGCTGAAAGAAATGTTGAGAAATGTTAATTCACAGGCTCTATTCCATGAAACATGCAGACCCATCAAACTTTTATATCATCCAAGGGAACCATTCTTTGCCCAGAAGATTCTCGTAATTTTCCCAATCCGTTTCAAGGAAAGTACAGATGTGATCATTGCAACAGCCTTTTTCCGGGAACTTATGGATGTGGGAAGCTCCGAAAAATGGGCAAAAGCACCTCCCTGCTGTTGGTCACCCATACCCCCGCCAGAACTGAGAGGCGAACCTTTGGAAGAACTGAGCACCAATGGAGGATTTGTCACCTTCGATATTTCTCTGCGTCACGTTGAAGGTAAAAGACTTGACAAAACTGTATGGAGTTTGCTGAACTTTAATGCATATGTGAAGTACCATGTAAAGACAACCAGAGGTTTTATCCAGAGAAGGATGAGAAAGCGGTTAGAAGGGCTTGTTGAGATCCTATATCAGAAAGGTTCAGATGATGTCCTACTCAACAAGGGTCAAGGCTGGGGGACTTCGATCTGTGGCGACGACAGCGCCATGCTGATCAAGTCGGTCCCGGCACAGGTTCGAGTCACAAAGCCGGTGAGATTCCGGCCAATGATGAAGAATGTTAATGAAGGGAAGGGTCTGTTTGCGCCTGTGGTAGTTCTTGCTAGAAATATTATTGGAAAGAAACGTTTCAATCAGTTGAGAGGCAAGGCCATTGCCCTGCACTCACAGATAATTACTGAGTTCTGCAAATCTATAGGAGCTGATGCAAAGCAAAGGCAGGGGTTGATTAGGTTAGCCAAGAAGAATGGAGAAAGATTAGGATTCCTTGCT

Coding sequence (CDS)

ATGGCATTCTTTCAGAGAGCTTCACCAGCTTTGAAGGAAATCCTCCTCAAACTGTGCAGTTTGGAAAAGCCCACAGAAATTGAGCATCACTTGCATGAATTTGGTTCTGTTCAATACCTTATCCAGTTCTCAGTATCGGATCCACAGAATATTTACCTGTCGATTTCAACCCCATTGTTGTCACAAGGAGCTCTGTTGTCTGATGGGCTCTCATCGCACACTGTCGAGATGGTAAAACAAATTTGCTCTCATGTTGTTGAGATCGTTGAACCTGCAAGAGAAGGATACCAACTTACTCTAAAAATCGATTTTGCACAAATTTTGCATGGGAAAGAGTCGGAAAAGATAATTACAGAGATTGCTGCTGTCCAAGCAGTGATTCTAAGTTCTCAGCTGAAAGAAATGTTGAGAAATGTTAATTCACAGGCTCTATTCCATGAAACATGCAGACCCATCAAACTTTTATATCATCCAAGGGAACCATTCTTTGCCCAGAAGATTCTCGTAATTTTCCCAATCCGTTTCAAGGAAAGTACAGATGTGATCATTGCAACAGCCTTTTTCCGGGAACTTATGGATGTGGGAAGCTCCGAAAAATGGGCAAAAGCACCTCCCTGCTGTTGGTCACCCATACCCCCGCCAGAACTGAGAGGCGAACCTTTGGAAGAACTGAGCACCAATGGAGGATTTGTCACCTTCGATATTTCTCTGCGTCACGTTGAAGGTAAAAGACTTGACAAAACTGTATGGAGTTTGCTGAACTTTAATGCATATGTGAAGTACCATGTAAAGACAACCAGAGGTTTTATCCAGAGAAGGATGAGAAAGCGGTTAGAAGGGCTTGTTGAGATCCTATATCAGAAAGGTTCAGATGATGTCCTACTCAACAAGGGTCAAGGCTGGGGGACTTCGATCTGTGGCGACGACAGCGCCATGCTGATCAAGTCGGTCCCGGCACAGGTTCGAGTCACAAAGCCGGTGAGATTCCGGCCAATGATGAAGAATGTTAATGAAGGGAAGGGTCTGTTTGCGCCTGTGGTAGTTCTTGCTAGAAATATTATTGGAAAGAAACGTTTCAATCAGTTGAGAGGCAAGGCCATTGCCCTGCACTCACAGATAATTACTGAGTTCTGCAAATCTATAGGAGCTGATGCAAAGCAAAGGCAGGGGTTGATTAGGTTAGCCAAGAAGAATGGAGAAAGATTAGGATTCCTTGCT

Protein sequence

MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFFAQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLNKGQGWGTSICGDDSAMLIKSVPAQVRVTKPVRFRPMMKNVNEGKGLFAPVVVLARNIIGKKRFNQLRGKAIALHSQIITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA
Homology
BLAST of MS010425 vs. NCBI nr
Match: XP_022154858.1 (actin-related protein 2/3 complex subunit 2B isoform X3 [Momordica charantia])

HSP 1 Score: 587.4 bits (1513), Expect = 9.3e-164
Identity = 298/306 (97.39%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL
Sbjct: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI
Sbjct: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFF----AQKILVIFPIRFK 180
           AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL+YHPREPFF    AQKILVIFPIRFK
Sbjct: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLVYHPREPFFVIKQAQKILVIFPIRFK 180

Query: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240
           ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS
Sbjct: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240

Query: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLN 300
           LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSD+VLLN
Sbjct: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDNVLLN 300

Query: 301 KGQGWG 303
           KGQ  G
Sbjct: 301 KGQDAG 306

BLAST of MS010425 vs. NCBI nr
Match: XP_038883860.1 (actin-related protein 2/3 complex subunit 2B isoform X3 [Benincasa hispida])

HSP 1 Score: 514.6 bits (1324), Expect = 7.7e-142
Identity = 262/311 (84.24%), Postives = 283/311 (91.00%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA FQRASPALKEILLKL SLEKPTE+EHHLHEFGSVQY IQ SVS+PQ I+LSI+TPLL
Sbjct: 1   MACFQRASPALKEILLKLYSLEKPTEMEHHLHEFGSVQYHIQSSVSNPQQIFLSIATPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQ  LLSDGLS +TVEMVKQICSH VEI+EPA+EGYQLTL+ID A+ILHG+ESEKIIT+I
Sbjct: 61  SQAVLLSDGLSPYTVEMVKQICSHAVEIIEPAKEGYQLTLRIDSAKILHGEESEKIITDI 120

Query: 121 AAVQAVILSSQLKEMLRN-VNSQALFHETCRPIKLLYHPREPFFA----QKILVIFPIRF 180
           AAVQAVI+SSQLKE+LRN VNS A F  T RPIKL+YHPREPFF     QKILVI+PIRF
Sbjct: 121 AAVQAVIISSQLKEVLRNVVNSPAHFQGTSRPIKLVYHPREPFFVIKQPQKILVIYPIRF 180

Query: 181 KESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI 240
           KE TDVIIATAFFRELMDVGSSEKW+KAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI
Sbjct: 181 KEDTDVIIATAFFRELMDVGSSEKWSKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI 240

Query: 241 SLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLL 300
           SL HVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL+QK SDD +L
Sbjct: 241 SLHHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILHQKTSDDAVL 300

Query: 301 NKGQGWGTSIC 307
           NKG+G   ++C
Sbjct: 301 NKGRGNRNALC 311

BLAST of MS010425 vs. NCBI nr
Match: XP_038883843.1 (actin-related protein 2/3 complex subunit 2B isoform X1 [Benincasa hispida])

HSP 1 Score: 511.9 bits (1317), Expect = 5.0e-141
Identity = 261/305 (85.57%), Postives = 280/305 (91.80%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA FQRASPALKEILLKL SLEKPTE+EHHLHEFGSVQY IQ SVS+PQ I+LSI+TPLL
Sbjct: 1   MACFQRASPALKEILLKLYSLEKPTEMEHHLHEFGSVQYHIQSSVSNPQQIFLSIATPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQ  LLSDGLS +TVEMVKQICSH VEI+EPA+EGYQLTL+ID A+ILHG+ESEKIIT+I
Sbjct: 61  SQAVLLSDGLSPYTVEMVKQICSHAVEIIEPAKEGYQLTLRIDSAKILHGEESEKIITDI 120

Query: 121 AAVQAVILSSQLKEMLRN-VNSQALFHETCRPIKLLYHPREPFFA----QKILVIFPIRF 180
           AAVQAVI+SSQLKE+LRN VNS A F  T RPIKL+YHPREPFF     QKILVI+PIRF
Sbjct: 121 AAVQAVIISSQLKEVLRNVVNSPAHFQGTSRPIKLVYHPREPFFVIKQPQKILVIYPIRF 180

Query: 181 KESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI 240
           KE TDVIIATAFFRELMDVGSSEKW+KAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI
Sbjct: 181 KEDTDVIIATAFFRELMDVGSSEKWSKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDI 240

Query: 241 SLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLL 300
           SL HVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL+QK SDD +L
Sbjct: 241 SLHHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILHQKTSDDAVL 300

BLAST of MS010425 vs. NCBI nr
Match: XP_022154850.1 (actin-related protein 2/3 complex subunit 2B isoform X2 [Momordica charantia])

HSP 1 Score: 510.4 bits (1313), Expect = 1.5e-140
Identity = 258/270 (95.56%), Postives = 262/270 (97.04%), Query Frame = 0

Query: 35  GSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 94
           G+    +QFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE
Sbjct: 71  GNPPQTVQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 130

Query: 95  GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 154
           GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL
Sbjct: 131 GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 190

Query: 155 LYHPREPFF----AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 214
           +YHPREPFF    AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP
Sbjct: 191 VYHPREPFFVIKQAQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 250

Query: 215 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 274
           IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI
Sbjct: 251 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 310

Query: 275 QRRMRKRLEGLVEILYQKGSDDVLLNKGQG 301
           QRRMRKRLEGLVEILYQKGSD+VLLNKGQG
Sbjct: 311 QRRMRKRLEGLVEILYQKGSDNVLLNKGQG 340

BLAST of MS010425 vs. NCBI nr
Match: XP_004134045.2 (actin-related protein 2/3 complex subunit 2B [Cucumis sativus] >KGN56823.1 hypothetical protein Csa_011299 [Cucumis sativus])

HSP 1 Score: 509.2 bits (1310), Expect = 3.2e-140
Identity = 255/304 (83.88%), Postives = 278/304 (91.45%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA FQRASPALKEILLKL SLEKPTEIEHHLHE+GSVQY IQ SV DPQ+IYLSI+TPLL
Sbjct: 1   MACFQRASPALKEILLKLYSLEKPTEIEHHLHEYGSVQYHIQSSVPDPQHIYLSIATPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQG LLSDGLS +TVEMVKQICSH +EI+EPA+EGY+LTL+I+ A+ILHG+ESEKIIT+I
Sbjct: 61  SQGDLLSDGLSPYTVEMVKQICSHAIEIIEPAKEGYELTLRINSAKILHGEESEKIITDI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFFA----QKILVIFPIRFK 180
           AAVQAVI+SS+LKE+LRNVNS  LF    RPIKL+YHPREPFF     QKIL+I+PIRFK
Sbjct: 121 AAVQAVIISSRLKEVLRNVNSPTLFQGISRPIKLVYHPREPFFVVKQPQKILIIYPIRFK 180

Query: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240
           E TDVIIATAFFRELMDVGSSEKW+KAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS
Sbjct: 181 EDTDVIIATAFFRELMDVGSSEKWSKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240

Query: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLN 300
           L HVEGKRLD TVWSLLNFNAYVKYHVKTTRGFIQRRMR+RLEGLVEIL+QK SD  +LN
Sbjct: 241 LHHVEGKRLDNTVWSLLNFNAYVKYHVKTTRGFIQRRMRRRLEGLVEILHQKSSDVAVLN 300

BLAST of MS010425 vs. ExPASy Swiss-Prot
Match: F4IVU1 (Actin-related protein 2/3 complex subunit 2B OS=Arabidopsis thaliana OX=3702 GN=ARPC2B PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.0e-88
Identity = 162/294 (55.10%), Postives = 224/294 (76.19%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA+ +RASP LKE LLK+   EKP E++ H HEFGS++Y I++SVSDP  +++S ST L 
Sbjct: 1   MAYLERASPTLKETLLKIYRAEKPIEVDQHFHEFGSIEYHIKYSVSDPNIVHVSTSTLLE 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           +QGA+    +SS+T E++K I   V++IV+P R G+QLTL ++   I  GKE+ KIIT I
Sbjct: 61  TQGAVTLKEISSYTYEVIKNIGVGVIDIVDPPRLGFQLTLGLNLDNIPRGKEAIKIITRI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCR-----PIKLLYHPREPFFA----QKILVIF 180
           + +QA+ILSSQLKEMLR++N    F +  R     PI+++YHP EPF+     +KI  +F
Sbjct: 121 SELQAIILSSQLKEMLRSLN----FQDDSRSINNMPIRIVYHPSEPFYVFKQPEKITAVF 180

Query: 181 PIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFV 240
           P+ FK+++DV+IAT+FF+EL++VGS +   KAP C WSPIPP +LRGEP+++L+TN GFV
Sbjct: 181 PMNFKDNSDVVIATSFFQELVEVGSQKDMGKAPQCSWSPIPPLQLRGEPVQDLTTNSGFV 240

Query: 241 TFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL 286
           +FDI+ RH+EGKRLDKTVW+LLNF A  KYH+K +RG+IQRRMRKR+E LV++L
Sbjct: 241 SFDITSRHIEGKRLDKTVWNLLNFYACAKYHIKCSRGYIQRRMRKRMETLVKLL 290

BLAST of MS010425 vs. ExPASy Swiss-Prot
Match: Q8LGI3 (Actin-related protein 2/3 complex subunit 2A OS=Arabidopsis thaliana OX=3702 GN=ARPC2A PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 7.4e-55
Identity = 108/281 (38.43%), Postives = 178/281 (63.35%), Query Frame = 0

Query: 11  LKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGL 70
           L+ +L +  +L+K  E+++   EF  V+Y +Q ++ +P  + LS+S P     A+  DGL
Sbjct: 12  LQTLLTRAQNLDKAVELDYQWIEFDDVRYHVQVTMKNPNLLLLSVSLPNPPPEAMSFDGL 71

Query: 71  SSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSS 130
               +E +K       +I++P R+G+ LTLK++F+++   +  E+++T++A+++ V++ +
Sbjct: 72  PLGAIEAIKTTYGTGFQILDPPRDGFSLTLKLNFSKV---RPDEELLTKLASIREVVMGA 131

Query: 131 QLKEMLRNVNSQALFHETCRPIKLLYHPREPFF----AQKILVIFPIRFKESTDVIIATA 190
            LK + +++ S+ +  E  R + +++ P E FF    A K+ V FP+RFK+S D I+AT+
Sbjct: 132 PLKIIFKHLASRTVAPELDRLVAIMHRPNETFFLVPQADKVTVAFPMRFKDSVDTILATS 191

Query: 191 FFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLD 250
           F +E ++   +     AP C WSP  P EL G P E LS N GFVTF I  RHVEGK+LD
Sbjct: 192 FLKEFVEARRAAALNTAPSCSWSPTAPQELEGAPKETLSANAGFVTFVIFPRHVEGKKLD 251

Query: 251 KTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQ 288
           +TVW+L  F+AYV YHVK + GF+  RMR+R+E +++ L Q
Sbjct: 252 RTVWNLSTFHAYVSYHVKFSEGFMHTRMRRRVESMIQALDQ 289

BLAST of MS010425 vs. ExPASy Swiss-Prot
Match: Q9SL05 (Protein PROTON GRADIENT REGULATION 5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGR5 PE=1 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 4.0e-40
Identity = 87/111 (78.38%), Postives = 97/111 (87.39%), Query Frame = 0

Query: 300 GWGTSICGDD-SAMLIKSV--PAQVRVT-KPVRFRPMMKNVNEGKGLFAPVVVLARNIIG 359
           GWG+SI G+D   ML K+V  P Q RV+ K +R  PMMKNVNEGKGLFAP+VV+ RN++G
Sbjct: 23  GWGSSISGEDYQTMLSKTVAPPQQARVSRKAIRAVPMMKNVNEGKGLFAPLVVVTRNLVG 82

Query: 360 KKRFNQLRGKAIALHSQIITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA 407
           KKRFNQLRGKAIALHSQ+ITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA
Sbjct: 83  KKRFNQLRGKAIALHSQVITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA 133

BLAST of MS010425 vs. ExPASy Swiss-Prot
Match: Q9CVB6 (Actin-related protein 2/3 complex subunit 2 OS=Mus musculus OX=10090 GN=Arpc2 PE=1 SV=3)

HSP 1 Score: 68.6 bits (166), Expect = 1.9e-10
Identity = 64/280 (22.86%), Postives = 117/280 (41.79%), Query Frame = 0

Query: 23  KPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTV-EMVKQI 82
           KP  +E    +F  V Y I     D   + +SIS     +       L +H   E++K++
Sbjct: 27  KPEAVEVTFADFDGVLYHISNPNGDKTKVMVSISLKFYKE-------LQAHGADELLKRV 86

Query: 83  CSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSS------QLKEM 142
               +   EP   GY ++L  D   +   K+S  I+ +   ++    +S      Q +E 
Sbjct: 87  YGSFLVNPEP---GYNVSLLYDLENLPASKDS--IVHQAGMLKRNCFASVFEKYFQFQEE 146

Query: 143 LRNVNSQALFHETCRPIKLLYHPREPFFAQ----KILVIFPIRFKESTDVIIATAFFREL 202
            +   ++A+ H         Y   E  + +    ++ V+F   FK+  DV+I   F +E 
Sbjct: 147 GKEGENRAVIH---------YRDDETMYVESKKDRVTVVFSTVFKDDDDVVIGKVFMQEF 206

Query: 203 MDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWS 262
            +   +     AP   +S   PP    +    +  N G++TF +  RH      D T+  
Sbjct: 207 KEGRRASH--TAPQVLFSHREPPLELKDTDAAVGDNIGYITFVLFPRHTNATARDNTINL 266

Query: 263 LLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSD 292
           +  F  Y+ YH+K ++ +I  RMR +    +++L +   D
Sbjct: 267 IHTFRDYLHYHIKCSKAYIHTRMRAKTSDFLKVLNRARPD 283

BLAST of MS010425 vs. ExPASy Swiss-Prot
Match: Q8WTM6 (Probable actin-related protein 2/3 complex subunit 2 OS=Caenorhabditis elegans OX=6239 GN=arx-4 PE=3 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 4.3e-10
Identity = 63/270 (23.33%), Postives = 121/270 (44.81%), Query Frame = 0

Query: 23  KPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTV-EMVKQI 82
           KP  +     +F  V Y +     D   I LSIS    ++       L  H   ++++++
Sbjct: 27  KPESVNVTFADFDGVLYKLSNPDGDRTKIILSISLKFYTE-------LQQHGADDLLRRV 86

Query: 83  C-SHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVN 142
              H+    E   +G+ +TL+ + A +    ++  ++   +A++    +S  ++      
Sbjct: 87  YGGHMRSTPE---QGFNVTLEYNLADL--PADTTDLVQAASALKRNCFASVFEKYF---E 146

Query: 143 SQALFHETCRPIKLLYHPREPFF----AQKILVIFPIRFKESTDVIIATAFFRELMDVGS 202
            Q    E  +   + Y   E  +    A ++ VIF   FK++ DVII   F +E  +   
Sbjct: 147 FQEAGQEGHKRAVINYRDDETMYIEAKADRVTVIFSTVFKDADDVIIGKVFLQEFREGRK 206

Query: 203 SEKWAKAPPCCWS-PIPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNF 262
           + +   AP   +S   PP EL+  P   +  N G++TF +  RH   K  D T+  + +F
Sbjct: 207 ASQ--TAPAVLYSLGEPPLELKDLPEARVGDNVGYITFVLFPRHTNKKTKDNTIDLIHSF 266

Query: 263 NAYVKYHVKTTRGFIQRRMRKRLEGLVEIL 286
             Y+ YH+K ++ ++  RMR +    +++L
Sbjct: 267 RDYLHYHIKCSKVYLHTRMRAKTTDFLKVL 279

BLAST of MS010425 vs. ExPASy TrEMBL
Match: A0A6J1DLF7 (Arp2/3 complex 34 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111022004 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 4.5e-164
Identity = 298/306 (97.39%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL
Sbjct: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI
Sbjct: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFF----AQKILVIFPIRFK 180
           AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL+YHPREPFF    AQKILVIFPIRFK
Sbjct: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLVYHPREPFFVIKQAQKILVIFPIRFK 180

Query: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240
           ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS
Sbjct: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240

Query: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLN 300
           LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSD+VLLN
Sbjct: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDNVLLN 300

Query: 301 KGQGWG 303
           KGQ  G
Sbjct: 301 KGQDAG 306

BLAST of MS010425 vs. ExPASy TrEMBL
Match: A0A6J1DKT8 (Arp2/3 complex 34 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111022004 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 7.0e-141
Identity = 258/270 (95.56%), Postives = 262/270 (97.04%), Query Frame = 0

Query: 35  GSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 94
           G+    +QFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE
Sbjct: 71  GNPPQTVQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 130

Query: 95  GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 154
           GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL
Sbjct: 131 GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 190

Query: 155 LYHPREPFF----AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 214
           +YHPREPFF    AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP
Sbjct: 191 VYHPREPFFVIKQAQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 250

Query: 215 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 274
           IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI
Sbjct: 251 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 310

Query: 275 QRRMRKRLEGLVEILYQKGSDDVLLNKGQG 301
           QRRMRKRLEGLVEILYQKGSD+VLLNKGQG
Sbjct: 311 QRRMRKRLEGLVEILYQKGSDNVLLNKGQG 340

BLAST of MS010425 vs. ExPASy TrEMBL
Match: A0A0A0L4Y8 (Arp2/3 complex 34 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_3G134720 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.6e-140
Identity = 255/304 (83.88%), Postives = 278/304 (91.45%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA FQRASPALKEILLKL SLEKPTEIEHHLHE+GSVQY IQ SV DPQ+IYLSI+TPLL
Sbjct: 1   MACFQRASPALKEILLKLYSLEKPTEIEHHLHEYGSVQYHIQSSVPDPQHIYLSIATPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQG LLSDGLS +TVEMVKQICSH +EI+EPA+EGY+LTL+I+ A+ILHG+ESEKIIT+I
Sbjct: 61  SQGDLLSDGLSPYTVEMVKQICSHAIEIIEPAKEGYELTLRINSAKILHGEESEKIITDI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFFA----QKILVIFPIRFK 180
           AAVQAVI+SS+LKE+LRNVNS  LF    RPIKL+YHPREPFF     QKIL+I+PIRFK
Sbjct: 121 AAVQAVIISSRLKEVLRNVNSPTLFQGISRPIKLVYHPREPFFVVKQPQKILIIYPIRFK 180

Query: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240
           E TDVIIATAFFRELMDVGSSEKW+KAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS
Sbjct: 181 EDTDVIIATAFFRELMDVGSSEKWSKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240

Query: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLN 300
           L HVEGKRLD TVWSLLNFNAYVKYHVKTTRGFIQRRMR+RLEGLVEIL+QK SD  +LN
Sbjct: 241 LHHVEGKRLDNTVWSLLNFNAYVKYHVKTTRGFIQRRMRRRLEGLVEILHQKSSDVAVLN 300

BLAST of MS010425 vs. ExPASy TrEMBL
Match: A0A6J1DKT4 (actin-related protein 2/3 complex subunit 2B isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022004 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 2.0e-140
Identity = 258/272 (94.85%), Postives = 262/272 (96.32%), Query Frame = 0

Query: 35  GSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 94
           G+    +QFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE
Sbjct: 71  GNPPQTVQFSVSDPQNIYLSISTPLLSQGALLSDGLSSHTVEMVKQICSHVVEIVEPARE 130

Query: 95  GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 154
           GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL
Sbjct: 131 GYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSSQLKEMLRNVNSQALFHETCRPIKL 190

Query: 155 LYHPREPFF----AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 214
           +YHPREPFF    AQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP
Sbjct: 191 VYHPREPFFVIKQAQKILVIFPIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSP 250

Query: 215 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 274
           IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI
Sbjct: 251 IPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFI 310

Query: 275 QRRMRKRLEGLVEILYQKGSDDVLLNKGQGWG 303
           QRRMRKRLEGLVEILYQKGSD+VLLNKGQ  G
Sbjct: 311 QRRMRKRLEGLVEILYQKGSDNVLLNKGQDAG 342

BLAST of MS010425 vs. ExPASy TrEMBL
Match: A0A1S3AWJ6 (Arp2/3 complex 34 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103483557 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.9e-139
Identity = 256/304 (84.21%), Postives = 276/304 (90.79%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA FQRASPALKEILLKL SLEKPTEIEHHLHE+GSVQY IQ SV DPQ+IYLSI+TPLL
Sbjct: 1   MACFQRASPALKEILLKLYSLEKPTEIEHHLHEYGSVQYHIQSSVPDPQHIYLSIATPLL 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           SQG LLSDGLS +TVEMVKQI SH VEI+EPA+EGY+LTL+ID A+ILHG+ESEKIIT+I
Sbjct: 61  SQGVLLSDGLSPYTVEMVKQIFSHAVEIIEPAKEGYELTLRIDSAKILHGEESEKIITDI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCRPIKLLYHPREPFFA----QKILVIFPIRFK 180
           AAVQAVI+SSQLKE+LRNVNS  LF    RPIKL+YHPREPFF     QKIL+I+PIRFK
Sbjct: 121 AAVQAVIISSQLKEVLRNVNSPTLFQGISRPIKLVYHPREPFFVVKQPQKILIIYPIRFK 180

Query: 181 ESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDIS 240
           E TDVIIATAFFRELMDVGSSEKW++APPC WSPIPPPELRGEPLEELSTNGGFVTFDIS
Sbjct: 181 EDTDVIIATAFFRELMDVGSSEKWSEAPPCGWSPIPPPELRGEPLEELSTNGGFVTFDIS 240

Query: 241 LRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQKGSDDVLLN 300
           L HVEGKRLD TVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL+QK SD  +LN
Sbjct: 241 LHHVEGKRLDNTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILHQKSSDVAVLN 300

BLAST of MS010425 vs. TAIR 10
Match: AT2G33385.2 (actin-related protein C2B )

HSP 1 Score: 327.0 bits (837), Expect = 2.1e-89
Identity = 162/294 (55.10%), Postives = 224/294 (76.19%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA+ +RASP LKE LLK+   EKP E++ H HEFGS++Y I++SVSDP  +++S ST L 
Sbjct: 1   MAYLERASPTLKETLLKIYRAEKPIEVDQHFHEFGSIEYHIKYSVSDPNIVHVSTSTLLE 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           +QGA+    +SS+T E++K I   V++IV+P R G+QLTL ++   I  GKE+ KIIT I
Sbjct: 61  TQGAVTLKEISSYTYEVIKNIGVGVIDIVDPPRLGFQLTLGLNLDNIPRGKEAIKIITRI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCR-----PIKLLYHPREPFFA----QKILVIF 180
           + +QA+ILSSQLKEMLR++N    F +  R     PI+++YHP EPF+     +KI  +F
Sbjct: 121 SELQAIILSSQLKEMLRSLN----FQDDSRSINNMPIRIVYHPSEPFYVFKQPEKITAVF 180

Query: 181 PIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFV 240
           P+ FK+++DV+IAT+FF+EL++VGS +   KAP C WSPIPP +LRGEP+++L+TN GFV
Sbjct: 181 PMNFKDNSDVVIATSFFQELVEVGSQKDMGKAPQCSWSPIPPLQLRGEPVQDLTTNSGFV 240

Query: 241 TFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL 286
           +FDI+ RH+EGKRLDKTVW+LLNF A  KYH+K +RG+IQRRMRKR+E LV++L
Sbjct: 241 SFDITSRHIEGKRLDKTVWNLLNFYACAKYHIKCSRGYIQRRMRKRMETLVKLL 290

BLAST of MS010425 vs. TAIR 10
Match: AT2G33385.1 (actin-related protein C2B )

HSP 1 Score: 310.5 bits (794), Expect = 2.1e-84
Identity = 157/294 (53.40%), Postives = 218/294 (74.15%), Query Frame = 0

Query: 1   MAFFQRASPALKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLL 60
           MA+ +RASP LKE LLK+   EKP E++ H HEFGS++Y I++SVSDP  +++S ST L 
Sbjct: 1   MAYLERASPTLKETLLKIYRAEKPIEVDQHFHEFGSIEYHIKYSVSDPNIVHVSTSTLLE 60

Query: 61  SQGALLSDGLSSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEI 120
           +QGA+    +SS+T E++K I   V++IV+P R G+QLTL ++   I  GKE+ KIIT I
Sbjct: 61  TQGAVTLKEISSYTYEVIKNIGVGVIDIVDPPRLGFQLTLGLNLDNIPRGKEAIKIITRI 120

Query: 121 AAVQAVILSSQLKEMLRNVNSQALFHETCR-----PIKLLYHPREPFFA----QKILVIF 180
           + +QA+ILSSQLKEMLR++N    F +  R     PI+++YHP EPF+     +KI  +F
Sbjct: 121 SELQAIILSSQLKEMLRSLN----FQDDSRSINNMPIRIVYHPSEPFYVFKQPEKITAVF 180

Query: 181 PIRFKESTDVIIATAFFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFV 240
           P+ FK+++DV+IAT+FF+++          KAP C WSPIPP +LRGEP+++L+TN GFV
Sbjct: 181 PMNFKDNSDVVIATSFFQDM---------GKAPQCSWSPIPPLQLRGEPVQDLTTNSGFV 240

Query: 241 TFDISLRHVEGKRLDKTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEIL 286
           +FDI+ RH+EGKRLDKTVW+LLNF A  KYH+K +RG+IQRRMRKR+E LV++L
Sbjct: 241 SFDITSRHIEGKRLDKTVWNLLNFYACAKYHIKCSRGYIQRRMRKRMETLVKLL 281

BLAST of MS010425 vs. TAIR 10
Match: AT1G30825.1 (Arp2/3 complex, 34 kD subunit p34-Arc )

HSP 1 Score: 216.1 bits (549), Expect = 5.3e-56
Identity = 108/281 (38.43%), Postives = 178/281 (63.35%), Query Frame = 0

Query: 11  LKEILLKLCSLEKPTEIEHHLHEFGSVQYLIQFSVSDPQNIYLSISTPLLSQGALLSDGL 70
           L+ +L +  +L+K  E+++   EF  V+Y +Q ++ +P  + LS+S P     A+  DGL
Sbjct: 12  LQTLLTRAQNLDKAVELDYQWIEFDDVRYHVQVTMKNPNLLLLSVSLPNPPPEAMSFDGL 71

Query: 71  SSHTVEMVKQICSHVVEIVEPAREGYQLTLKIDFAQILHGKESEKIITEIAAVQAVILSS 130
               +E +K       +I++P R+G+ LTLK++F+++   +  E+++T++A+++ V++ +
Sbjct: 72  PLGAIEAIKTTYGTGFQILDPPRDGFSLTLKLNFSKV---RPDEELLTKLASIREVVMGA 131

Query: 131 QLKEMLRNVNSQALFHETCRPIKLLYHPREPFF----AQKILVIFPIRFKESTDVIIATA 190
            LK + +++ S+ +  E  R + +++ P E FF    A K+ V FP+RFK+S D I+AT+
Sbjct: 132 PLKIIFKHLASRTVAPELDRLVAIMHRPNETFFLVPQADKVTVAFPMRFKDSVDTILATS 191

Query: 191 FFRELMDVGSSEKWAKAPPCCWSPIPPPELRGEPLEELSTNGGFVTFDISLRHVEGKRLD 250
           F +E ++   +     AP C WSP  P EL G P E LS N GFVTF I  RHVEGK+LD
Sbjct: 192 FLKEFVEARRAAALNTAPSCSWSPTAPQELEGAPKETLSANAGFVTFVIFPRHVEGKKLD 251

Query: 251 KTVWSLLNFNAYVKYHVKTTRGFIQRRMRKRLEGLVEILYQ 288
           +TVW+L  F+AYV YHVK + GF+  RMR+R+E +++ L Q
Sbjct: 252 RTVWNLSTFHAYVSYHVKFSEGFMHTRMRRRVESMIQALDQ 289

BLAST of MS010425 vs. TAIR 10
Match: AT2G05620.1 (proton gradient regulation 5 )

HSP 1 Score: 167.2 bits (422), Expect = 2.8e-41
Identity = 87/111 (78.38%), Postives = 97/111 (87.39%), Query Frame = 0

Query: 300 GWGTSICGDD-SAMLIKSV--PAQVRVT-KPVRFRPMMKNVNEGKGLFAPVVVLARNIIG 359
           GWG+SI G+D   ML K+V  P Q RV+ K +R  PMMKNVNEGKGLFAP+VV+ RN++G
Sbjct: 23  GWGSSISGEDYQTMLSKTVAPPQQARVSRKAIRAVPMMKNVNEGKGLFAPLVVVTRNLVG 82

Query: 360 KKRFNQLRGKAIALHSQIITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA 407
           KKRFNQLRGKAIALHSQ+ITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA
Sbjct: 83  KKRFNQLRGKAIALHSQVITEFCKSIGADAKQRQGLIRLAKKNGERLGFLA 133

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154858.19.3e-16497.39actin-related protein 2/3 complex subunit 2B isoform X3 [Momordica charantia][more]
XP_038883860.17.7e-14284.24actin-related protein 2/3 complex subunit 2B isoform X3 [Benincasa hispida][more]
XP_038883843.15.0e-14185.57actin-related protein 2/3 complex subunit 2B isoform X1 [Benincasa hispida][more]
XP_022154850.11.5e-14095.56actin-related protein 2/3 complex subunit 2B isoform X2 [Momordica charantia][more]
XP_004134045.23.2e-14083.88actin-related protein 2/3 complex subunit 2B [Cucumis sativus] >KGN56823.1 hypot... [more]
Match NameE-valueIdentityDescription
F4IVU13.0e-8855.10Actin-related protein 2/3 complex subunit 2B OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8LGI37.4e-5538.43Actin-related protein 2/3 complex subunit 2A OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SL054.0e-4078.38Protein PROTON GRADIENT REGULATION 5, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9CVB61.9e-1022.86Actin-related protein 2/3 complex subunit 2 OS=Mus musculus OX=10090 GN=Arpc2 PE... [more]
Q8WTM64.3e-1023.33Probable actin-related protein 2/3 complex subunit 2 OS=Caenorhabditis elegans O... [more]
Match NameE-valueIdentityDescription
A0A6J1DLF74.5e-16497.39Arp2/3 complex 34 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111022004 PE=... [more]
A0A6J1DKT87.0e-14195.56Arp2/3 complex 34 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111022004 PE=... [more]
A0A0A0L4Y81.6e-14083.88Arp2/3 complex 34 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_3G134720 PE=3 SV... [more]
A0A6J1DKT42.0e-14094.85actin-related protein 2/3 complex subunit 2B isoform X1 OS=Momordica charantia O... [more]
A0A1S3AWJ63.9e-13984.21Arp2/3 complex 34 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103483557 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G33385.22.1e-8955.10actin-related protein C2B [more]
AT2G33385.12.1e-8453.40actin-related protein C2B [more]
AT1G30825.15.3e-5638.43Arp2/3 complex, 34 kD subunit p34-Arc [more]
AT2G05620.12.8e-4178.38proton gradient regulation 5 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR034666Arp2/3 complex subunit 2/4GENE3D3.30.1460.20coord: 145..290
e-value: 1.7E-40
score: 140.3
IPR034666Arp2/3 complex subunit 2/4GENE3D3.30.1460.20coord: 1..137
e-value: 1.3E-27
score: 98.6
IPR034666Arp2/3 complex subunit 2/4SUPERFAMILY69645Arp2/3 complex subunitscoord: 1..126
IPR034666Arp2/3 complex subunit 2/4SUPERFAMILY69645Arp2/3 complex subunitscoord: 133..287
IPR007188Actin-related protein 2/3 complex subunit 2PFAMPF04045P34-Arccoord: 78..285
e-value: 2.2E-28
score: 99.6
IPR007188Actin-related protein 2/3 complex subunit 2PANTHERPTHR12058ARP2/3 COMPLEX 34 KDA SUBUNITcoord: 1..296
NoneNo IPR availablePANTHERPTHR12058:SF1ACTIN-RELATED PROTEIN 2/3 COMPLEX SUBUNIT 2Bcoord: 1..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010425.1MS010425.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030041 actin filament polymerization
biological_process GO:0034314 Arp2/3 complex-mediated actin nucleation
biological_process GO:0030833 regulation of actin filament polymerization
cellular_component GO:0015629 actin cytoskeleton
cellular_component GO:0005885 Arp2/3 protein complex