MS010377 (gene) Bitter gourd (TR) v1

Overview


Name	MS010377
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	enoyl-CoA delta isomerase 2, peroxisomal-like
Location	scaffold878: 514726 .. 515442 (+)
RNA-Seq Expression	MS010377
Synteny	MS010377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCACAGTTGAGAAGCGAGGCAATTTGTTCATCCTGACTCTGACCGGAGACGACGAGCACCGCCTCAGCCCATCCTTAATCGACTCCATCCTCGCCGCTCTTTCCCAAATCAGGGCCCAAGCCTCCCCCGGCTCCGTTCTCATCACCACTTCCCATGGCAAATTCTTCTCCAACGGCTTCGATCTCCGCTGGGCTCACGCCGCCGGCTCTGTTTCTGCGGCTGCCGACCGCCTGAGCCACATGGTTCACATCTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTTCCCATGCCTTCCATAGCCGCGATTTCAGGCCACGCCGCCGCTGCCGGTTTCATTCTGGCCCTCAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAGGTCGATCTAGGGCTAACTCTCCCGGACTACTTCACGGCGCTCTTTAATTCCAAAATCGGCTCCGGTTCCGTCCGCCGCGATGTGTTCCTGAGGGGAATGAAAGTGAAAGGCGAGGCGGCGGTGAGGATGGGCATCGTGGAATCCGCCCACGACGGCGACGACGGCGCGATGAAGGCTGCGCTCCGCCTTGGGGAGCAGCTGGCGGCCAGAAATTGGGAGGGCGAACCCTACGCGGAAATCAGGAAGAGTCTGTATCCGGGGATCTCTCGGCTGCTTGGATTACCGGAAAAGGTCATTTCGCTTTCGAAGCTC

mRNA sequence

ATGTGCACAGTTGAGAAGCGAGGCAATTTGTTCATCCTGACTCTGACCGGAGACGACGAGCACCGCCTCAGCCCATCCTTAATCGACTCCATCCTCGCCGCTCTTTCCCAAATCAGGGCCCAAGCCTCCCCCGGCTCCGTTCTCATCACCACTTCCCATGGCAAATTCTTCTCCAACGGCTTCGATCTCCGCTGGGCTCACGCCGCCGGCTCTGTTTCTGCGGCTGCCGACCGCCTGAGCCACATGGTTCACATCTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTTCCCATGCCTTCCATAGCCGCGATTTCAGGCCACGCCGCCGCTGCCGGTTTCATTCTGGCCCTCAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAGGTCGATCTAGGGCTAACTCTCCCGGACTACTTCACGGCGCTCTTTAATTCCAAAATCGGCTCCGGTTCCGTCCGCCGCGATGTGTTCCTGAGGGGAATGAAAGTGAAAGGCGAGGCGGCGGTGAGGATGGGCATCGTGGAATCCGCCCACGACGGCGACGACGGCGCGATGAAGGCTGCGCTCCGCCTTGGGGAGCAGCTGGCGGCCAGAAATTGGGAGGGCGAACCCTACGCGGAAATCAGGAAGAGTCTGTATCCGGGGATCTCTCGGCTGCTTGGATTACCGGAAAAGGTCATTTCGCTTTCGAAGCTC

Coding sequence (CDS)

ATGTGCACAGTTGAGAAGCGAGGCAATTTGTTCATCCTGACTCTGACCGGAGACGACGAGCACCGCCTCAGCCCATCCTTAATCGACTCCATCCTCGCCGCTCTTTCCCAAATCAGGGCCCAAGCCTCCCCCGGCTCCGTTCTCATCACCACTTCCCATGGCAAATTCTTCTCCAACGGCTTCGATCTCCGCTGGGCTCACGCCGCCGGCTCTGTTTCTGCGGCTGCCGACCGCCTGAGCCACATGGTTCACATCTTCAAGCCCGTCGTCGCCCAACTCCTCTCCCTTCCCATGCCTTCCATAGCCGCGATTTCAGGCCACGCCGCCGCTGCCGGTTTCATTCTGGCCCTCAGCCACGACTATTTGATTATGAGGAGCGATCGAGGCGTTCTGTACATGAGCGAGGTCGATCTAGGGCTAACTCTCCCGGACTACTTCACGGCGCTCTTTAATTCCAAAATCGGCTCCGGTTCCGTCCGCCGCGATGTGTTCCTGAGGGGAATGAAAGTGAAAGGCGAGGCGGCGGTGAGGATGGGCATCGTGGAATCCGCCCACGACGGCGACGACGGCGCGATGAAGGCTGCGCTCCGCCTTGGGGAGCAGCTGGCGGCCAGAAATTGGGAGGGCGAACCCTACGCGGAAATCAGGAAGAGTCTGTATCCGGGGATCTCTCGGCTGCTTGGATTACCGGAAAAGGTCATTTCGCTTTCGAAGCTC

Protein sequence

MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNGFDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHDYLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGIVESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL

Homology

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BLAST of MS010377 vs. NCBI nr
Match: XP_022145482.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia])

HSP 1 Score: 453.0 bits (1164), Expect = 1.6e-123
Identity = 232/233 (99.57%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD
Sbjct: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKV 234
           VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEK+
Sbjct: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKL 233

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BLAST of MS010377 vs. NCBI nr
Match: XP_038877347.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida])

HSP 1 Score: 403.3 bits (1035), Expect = 1.5e-108
Identity = 204/239 (85.36%), Postives = 219/239 (91.63%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGDD+HRLSPS I SIL ALS++++QASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDDDHRLSPSFIASILEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAADRL HMVHIFKP+VA+LLSLPMP+IAAI+GHAAAAGF LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAADRLMHMVHIFKPIVAELLSLPMPTIAAIAGHAAAAGFALALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YL MR D GVLYMSEVDLGL+LPDYFTALF SKIGS SVRRDV LRGMKVKGEAAVRMGI
Sbjct: 121 YLFMRRDLGVLYMSEVDLGLSLPDYFTALFKSKIGSSSVRRDVLLRGMKVKGEAAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VES HDG+DG M+AA+R GEQL ARNW GEPYAEIRKSLYP ISRLLGLPEKVIS+SKL
Sbjct: 181 VESVHDGEDGVMEAAVRFGEQLGARNWNGEPYAEIRKSLYPEISRLLGLPEKVISISKL 239

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BLAST of MS010377 vs. NCBI nr
Match: KAG6582370.1 (Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 403.3 bits (1035), Expect = 1.5e-108
Identity = 206/239 (86.19%), Postives = 220/239 (92.05%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILTLTGDDEHRLSPS I SIL ALS+++A+ASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSYIASILKALSKVKAEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAA+RL HMV IFKPVVA+LLS PMP+IAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YLIMRSDRGVLYMSEVDLGLTLPDYF+ALF SKIGS SVRRDV L GMKVKGE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVKGEPAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VESAH+G DG MKAA+RLGEQLA RNW GEPYAEIRKSL+P ISRLLGLPEKV S+SKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

[Close]

BLAST of MS010377 vs. NCBI nr
Match: XP_022924233.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 400.2 bits (1027), Expect = 1.2e-107
Identity = 204/239 (85.36%), Postives = 219/239 (91.63%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILTLTGDDEHRLSPS I SIL ALS+++++ASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAA+RL HMV IFKPVVA+LLS PMP+IAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YLIMRSDRGVLYMSEVDLGLTLPDYF+ALF SKIGS SVRRDV L GMKV GE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVTGEPAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VESAH+G DG MKAA+RLGEQLA RNW GEPYAEIRKSL+P ISRLLGLPEKV S+SKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

[Close]

BLAST of MS010377 vs. NCBI nr
Match: XP_022980470.1 (enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 399.8 bits (1026), Expect = 1.6e-107
Identity = 202/239 (84.52%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILT+TGDDEHRLSPS I SIL ALS+++++ASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTITGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAA+RL +MV IFKPVVA+LLSLPMP+IAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHYMVQIFKPVVAELLSLPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+IMRSDRGVLYMSEVDLGLTLPDYF+ALF SKIGS SVRRD+ L GMKVKGE+AVRMGI
Sbjct: 121 YMIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDLLLTGMKVKGESAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VESAH+G DG MKAA+RLGEQLA RNW GEPYAEIRKSLYP ISRLLGLPEK  S+SKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLYPEISRLLGLPEKFFSISKL 239

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BLAST of MS010377 vs. ExPASy Swiss-Prot
Match: O23299 (Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2 PE=1 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 5.1e-80
Identity = 148/240 (61.67%), Postives = 191/240 (79.58%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKRG+LF+LTLTGD EHR  P  I +IL+ L Q ++Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA  AGS + AA+RL  MV  FKPVVA LL LPMP+IAA++GHAAAAG ILALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+ MR DRGVLYMSEVD+GL++PDYF+AL  +KIG+ + RR++ L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           V+S A+D ++G + A++RLGE+LAA+ W GE YA IRKSLYP +  +LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

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BLAST of MS010377 vs. ExPASy Swiss-Prot
Match: O23300 (Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1)

HSP 1 Score: 282.3 bits (721), Expect = 5.0e-75
Identity = 141/230 (61.30%), Postives = 178/230 (77.39%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKRG+LF+LTLTG+DEHR  P  I S+L+ L Q ++Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA +AG    A  R+  MV  FKPV+A LL LPMP+IAA++GHAAA+G + ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+ MR DRGVLYMSEVD+GL +PDYF+AL  +K+GSG  RR++ L G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGL 230
           V+S AHD  +G ++A + LGE LAA+ W GE YA IRKSLYP + R++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of MS010377 vs. ExPASy Swiss-Prot
Match: O04469 (Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-54
Identity = 113/220 (51.36%), Postives = 156/220 (70.91%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQAS-PGSVLITTSHGKFFSN 60
           MC++EKR  LFIL LTGD EHRL+P+L+DS+ + ++QIR+  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSH 120
           G+DL  A +  S+S   D         + +VA L+SLPMP+IAA++GHA+AAG ILA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMG 180
           DY++MR DRG LYMSE+D+ L +P +F A+   KIGS + RRDV L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSL 220
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESL 214

[Close]

BLAST of MS010377 vs. ExPASy Swiss-Prot
Match: A9MR28 (Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=caiD PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 52/158 (32.91%), Postives = 80/158 (50.63%), Query Frame = 0


Query: 48  LITTSHGKFFSNGFDLRWAHAAGSVSAAADRLSHMVHIFKP----VVAQLLSLPMPSIAA 107
           +IT +  KFFS G+DL+   AA   +  AD        F P     + +L  L  P IAA
Sbjct: 51  IITGAGEKFFSAGWDLK--AAAEGEAPDAD--------FGPGGFAGLTELFDLNKPVIAA 110

Query: 108 ISGHAAAAGFILALSHDYLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDV 167
           ++G+A   GF LAL+ D++I  +D     + E  LG+ +PD    L   KI   ++  D+
Sbjct: 111 VNGYAFGGGFELALAADFIIC-ADHASFALPEAKLGI-VPDSGGVLRLPKILPPAIVNDM 170

Query: 168 FLRGMKVKGEAAVRMGIVE---SAHDGDDGAMKAALRL 199
            + G ++  E A+R G+V    S H+  D A + A +L
Sbjct: 171 VMTGRRMTAEEALRWGVVNRVVSPHELLDSARELARQL 196

[Close]

BLAST of MS010377 vs. ExPASy Swiss-Prot
Match: A8ALR7 (Carnitinyl-CoA dehydratase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696) OX=290338 GN=caiD PE=3 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-05
Identity = 48/159 (30.19%), Postives = 77/159 (48.43%), Query Frame = 0


Query: 48  LITTSHGKFFSNGFDLRWAHAAGSVSAAADRLSHMVHIFKP----VVAQLLSLPMPSIAA 107
           +IT    KFFS G+DL+   AA   +  AD        F P     + ++  L  P IAA
Sbjct: 51  IITGGGEKFFSAGWDLK--AAAEGEAPDAD--------FGPGGFAGLTEIFDLDKPVIAA 110

Query: 108 ISGHAAAAGFILALSHDYLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDV 167
           ++G+A   GF LAL+ D+++  +D     + E  LG+ +PD    L   K+   ++  ++
Sbjct: 111 VNGYAFGGGFELALAADFIVC-ADNASFALPEAKLGI-VPDSGGVLRLPKLLPPAIVNEM 170

Query: 168 FLRGMKVKGEAAVRMGIVESAHDGDDGAMKAALRLGEQL 203
            + G ++  E A+R GIV          M +A  L +QL
Sbjct: 171 LMTGRRMDAEEALRWGIVNRV-VSQQALMDSARELAQQL 196

[Close]

BLAST of MS010377 vs. ExPASy TrEMBL
Match: A0A6J1CWQ4 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=LOC111014917 PE=4 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 7.8e-124
Identity = 232/233 (99.57%), Postives = 233/233 (100.00%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD
Sbjct: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKV 234
           VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEK+
Sbjct: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKL 233

[Close]

BLAST of MS010377 vs. ExPASy TrEMBL
Match: A0A6J1EBT7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431760 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 6.0e-108
Identity = 204/239 (85.36%), Postives = 219/239 (91.63%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILTLTGDDEHRLSPS I SIL ALS+++++ASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTLTGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAA+RL HMV IFKPVVA+LLS PMP+IAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHHMVQIFKPVVAELLSFPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YLIMRSDRGVLYMSEVDLGLTLPDYF+ALF SKIGS SVRRDV L GMKV GE AVRMGI
Sbjct: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDVLLTGMKVTGEPAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VESAH+G DG MKAA+RLGEQLA RNW GEPYAEIRKSL+P ISRLLGLPEKV S+SKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLHPEISRLLGLPEKVFSISKL 239

[Close]

BLAST of MS010377 vs. ExPASy TrEMBL
Match: A0A6J1IWE7 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111479854 PE=4 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 7.9e-108
Identity = 202/239 (84.52%), Postives = 221/239 (92.47%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCTVEKRGNLFILT+TGDDEHRLSPS I SIL ALS+++++ASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTVEKRGNLFILTITGDDEHRLSPSFIASILKALSKVKSEASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA AAGSVSAAA+RL +MV IFKPVVA+LLSLPMP+IAAI+GHAAAAGF+LALSHD
Sbjct: 61  FDLSWAQAAGSVSAAAERLHYMVQIFKPVVAELLSLPMPTIAAIAGHAAAAGFLLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+IMRSDRGVLYMSEVDLGLTLPDYF+ALF SKIGS SVRRD+ L GMKVKGE+AVRMGI
Sbjct: 121 YMIMRSDRGVLYMSEVDLGLTLPDYFSALFKSKIGSSSVRRDLLLTGMKVKGESAVRMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           VESAH+G DG MKAA+RLGEQLA RNW GEPYAEIRKSLYP ISRLLGLPEK  S+SKL
Sbjct: 181 VESAHEGADGVMKAAVRLGEQLAERNWNGEPYAEIRKSLYPEISRLLGLPEKFFSISKL 239

[Close]

BLAST of MS010377 vs. ExPASy TrEMBL
Match: A0A1S3AWS8 (enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103483623 PE=4 SV=1)

HSP 1 Score: 389.0 bits (998), Expect = 1.4e-104
Identity = 194/239 (81.17%), Postives = 219/239 (91.63%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGD +HRL+PS+I ++L ALS++++QASPGSVLITTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLITTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WAHAAGSVSAAA+RL HMV  FKPV+A+LLSLPMP+IAAISGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YL+MRSDRGVLYMSEVDLGL+LPDYF ALF SKIGS SVRRDV +RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
            +SA+DG+ G M+AA+RLGEQL AR W GEPYAEIRKSLYP ISRLLGLPEK IS+SKL
Sbjct: 181 ADSAYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of MS010377 vs. ExPASy TrEMBL
Match: A0A5A7U6I8 (Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001360 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 5.3e-104
Identity = 192/239 (80.33%), Postives = 218/239 (91.21%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKR NLFILTLTGD +HRL+PS+I ++L ALS++++QASPGSVL+TTSHGKFFSNG
Sbjct: 1   MCTLEKRRNLFILTLTGDHDHRLNPSVIAALLEALSKVKSQASPGSVLVTTSHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WAHAAGSVSAAA+RL HMV  FKPV+A+LLSLPMP+IAAISGHAAAAGF+LALSHD
Sbjct: 61  FDLSWAHAAGSVSAAAERLHHMVQNFKPVIAELLSLPMPTIAAISGHAAAAGFMLALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           YL+MRSDRGVLYMSEVDLGL+LPDYF ALF SKIGS SVRRDV +RGMKVKGEAAV+MGI
Sbjct: 121 YLLMRSDRGVLYMSEVDLGLSLPDYFAALFKSKIGSSSVRRDVLMRGMKVKGEAAVKMGI 180

Query: 181 VESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
            +S +DG+ G M+AA+RLGEQL AR W GEPYAEIRKSLYP ISRLLGLPEK IS+SKL
Sbjct: 181 ADSVYDGESGVMEAAVRLGEQLGARKWNGEPYAEIRKSLYPEISRLLGLPEKAISISKL 239

[Close]

BLAST of MS010377 vs. TAIR 10
Match: AT4G14430.1 (indole-3-butyric acid response 10 )

HSP 1 Score: 298.9 bits (764), Expect = 3.6e-81
Identity = 148/240 (61.67%), Postives = 191/240 (79.58%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKRG+LF+LTLTGD EHR  P  I +IL+ L Q ++Q++ GS+LITT++GKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGDGEHRFHPDTIATILSLLEQAKSQSTRGSILITTANGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA  AGS + AA+RL  MV  FKPVVA LL LPMP+IAA++GHAAAAG ILALSHD
Sbjct: 61  FDLAWAQTAGSKTGAANRLHQMVESFKPVVAALLDLPMPTIAALNGHAAAAGLILALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+ MR DRGVLYMSEVD+GL++PDYF+AL  +KIG+ + RR++ L G K++GE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLSMPDYFSALVRAKIGTSAARRELLLSGKKIRGEEAVGLGI 180

Query: 181 VES-AHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGLPEKVISLSKL 240
           V+S A+D ++G + A++RLGE+LAA+ W GE YA IRKSLYP +  +LGL  +V +  KL
Sbjct: 181 VDSAAYDSEEGVVVASVRLGEKLAAKKWSGEVYASIRKSLYPELCGILGLETRVFATPKL 240

[Close]

BLAST of MS010377 vs. TAIR 10
Match: AT4G14440.1 (3-hydroxyacyl-CoA dehydratase 1 )

HSP 1 Score: 282.3 bits (721), Expect = 3.5e-76
Identity = 141/230 (61.30%), Postives = 178/230 (77.39%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQASPGSVLITTSHGKFFSNG 60
           MCT+EKRG+LF+LTLTG+DEHR  P  I S+L+ L Q ++Q++ GSVLITT HGKFFSNG
Sbjct: 1   MCTLEKRGDLFLLTLTGEDEHRFHPDTIASVLSLLEQAKSQSTKGSVLITTGHGKFFSNG 60

Query: 61  FDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSHD 120
           FDL WA +AG    A  R+  MV  FKPV+A LL LPMP+IAA++GHAAA+G + ALSHD
Sbjct: 61  FDLAWAQSAG--HGAIKRMHQMVKSFKPVLAALLDLPMPTIAALNGHAAASGLMFALSHD 120

Query: 121 YLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMGI 180
           Y+ MR DRGVLYMSEVD+GL +PDYF+AL  +K+GSG  RR++ L G K+KGE AV +GI
Sbjct: 121 YVFMRKDRGVLYMSEVDIGLPVPDYFSALVVAKVGSGIARRELLLSGKKLKGEEAVALGI 180

Query: 181 VES-AHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSLYPGISRLLGL 230
           V+S AHD  +G ++A + LGE LAA+ W GE YA IRKSLYP + R++ L
Sbjct: 181 VDSAAHDSAEGVVEATVSLGESLAAKKWNGEVYATIRKSLYPELCRMVDL 228

[Close]

BLAST of MS010377 vs. TAIR 10
Match: AT1G65520.1 (delta(3), delta(2)-enoyl CoA isomerase 1 )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 113/220 (51.36%), Postives = 156/220 (70.91%), Query Frame = 0


Query: 1   MCTVEKRGNLFILTLTGDDEHRLSPSLIDSILAALSQIRAQAS-PGSVLITTSHGKFFSN 60
           MC++EKR  LFIL LTGD EHRL+P+L+DS+ + ++QIR+  S   SVLITTS GKFFSN
Sbjct: 1   MCSLEKRDRLFILKLTGDGEHRLNPTLLDSLRSTINQIRSDPSFSQSVLITTSDGKFFSN 60

Query: 61  GFDLRWAHAAGSVSAAADRLSHMVHIFKPVVAQLLSLPMPSIAAISGHAAAAGFILALSH 120
           G+DL  A +  S+S   D         + +VA L+SLPMP+IAA++GHA+AAG ILA+SH
Sbjct: 61  GYDLALAESNPSLSVVMDAK------LRSLVADLISLPMPTIAAVTGHASAAGCILAMSH 120

Query: 121 DYLIMRSDRGVLYMSEVDLGLTLPDYFTALFNSKIGSGSVRRDVFLRGMKVKGEAAVRMG 180
           DY++MR DRG LYMSE+D+ L +P +F A+   KIGS + RRDV L   KV  +  V+MG
Sbjct: 121 DYVLMRRDRGFLYMSELDIELIVPAWFMAVIRGKIGSPAARRDVMLTAAKVTADVGVKMG 180

Query: 181 IVESAHDGDDGAMKAALRLGEQLAARNWEGEPYAEIRKSL 220
           IV+SA+      ++AA++LGE++  R  +G  Y ++R+SL
Sbjct: 181 IVDSAYGSAAETVEAAIKLGEEIVQRGGDGHVYGKMRESL 214

The following BLAST results are available for this feature:

BLAST of MS010377 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022145482.1	1.6e-123	99.57	enoyl-CoA delta isomerase 2, peroxisomal-like [Momordica charantia]	[more]
XP_038877347.1	1.5e-108	85.36	enoyl-CoA delta isomerase 2, peroxisomal-like [Benincasa hispida]	[more]
KAG6582370.1	1.5e-108	86.19	Enoyl-CoA delta isomerase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022924233.1	1.2e-107	85.36	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita moschata]	[more]
XP_022980470.1	1.6e-107	84.52	enoyl-CoA delta isomerase 2, peroxisomal-like [Cucurbita maxima]	[more]

BLAST of MS010377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23299	5.1e-80	61.67	Enoyl-CoA delta isomerase 2, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI2...	[more]
O23300	5.0e-75	61.30	Enoyl-CoA delta isomerase 3 OS=Arabidopsis thaliana OX=3702 GN=ECI3 PE=1 SV=1	[more]
O04469	1.7e-54	51.36	Enoyl-CoA delta isomerase 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=ECI1...	[more]
A9MR28	1.3e-06	32.91	Carnitinyl-CoA dehydratase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-...	[more]
A8ALR7	1.9e-05	30.19	Carnitinyl-CoA dehydratase OS=Citrobacter koseri (strain ATCC BAA-895 / CDC 4225...	[more]

BLAST of MS010377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CWQ4	7.8e-124	99.57	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Momordica charantia OX=3673 GN=...	[more]
A0A6J1EBT7	6.0e-108	85.36	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1IWE7	7.9e-108	84.52	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A1S3AWS8	1.4e-104	81.17	enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5A7U6I8	5.3e-104	80.33	Enoyl-CoA delta isomerase 2, peroxisomal-like OS=Cucumis melo var. makuwa OX=119...	[more]

BLAST of MS010377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G14430.1	3.6e-81	61.67	indole-3-butyric acid response 10	[more]
AT4G14440.1	3.5e-76	61.30	3-hydroxyacyl-CoA dehydratase 1	[more]
AT1G65520.1	1.2e-55	51.36	delta(3), delta(2)-enoyl CoA isomerase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.90.226.10		coord: 1..227 e-value: 1.1E-37 score: 131.5
None	No IPR available	PANTHER	PTHR11941:SF75	ENOYL-COA DELTA ISOMERASE 2, PEROXISOMAL	coord: 1..232
None	No IPR available	PANTHER	PTHR11941	ENOYL-COA HYDRATASE-RELATED	coord: 1..232
None	No IPR available	CDD	cd06558	crotonase-like	coord: 2..202 e-value: 7.10715E-23 score: 89.9272
IPR001753	Enoyl-CoA hydratase/isomerase	PFAM	PF00378	ECH_1	coord: 23..204 e-value: 2.9E-20 score: 72.6
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 3..206

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010377.1	MS010377.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003824	catalytic activity