MS010316 (gene) Bitter gourd (TR) v1

BLAST of MS010316 vs. NCBI nr
Match: XP_022144568.1 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 617/621 (99.36%), Postives = 618/621 (99.52%), Query Frame = 0

Query: 7   MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP 66
           MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP
Sbjct: 1   MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP 60

Query: 67  PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG 126
           PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG
Sbjct: 61  PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG 120

Query: 127 IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG 186
           IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG
Sbjct: 121 IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG 180

Query: 187 ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD 246
           ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD
Sbjct: 181 ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD 240

Query: 247 SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ 306
           SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ
Sbjct: 241 SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ 300

Query: 307 CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFDNHLVIE 366
           CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR   D+HLVIE
Sbjct: 301 CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR---DSHLVIE 360

Query: 367 LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE 426
           LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE
Sbjct: 361 LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE 420

Query: 427 CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA 486
           CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA
Sbjct: 421 CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA 480

Query: 487 RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI 546
           RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI
Sbjct: 481 RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI 540

Query: 547 FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA 606
           FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA
Sbjct: 541 FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA 600

Query: 607 RLEMMRREHAHLKAQLRDFEE 628
           RLEMMRREHAHLKAQLRDFEE
Sbjct: 601 RLEMMRREHAHLKAQLRDFEE 618

BLAST of MS010316 vs. NCBI nr
Match: XP_038874389.1 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 971.5 bits (2510), Expect = 3.6e-279
Identity = 506/628 (80.57%), Postives = 554/628 (88.22%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLP+  SPDSAS Y PESSSS A RISD +E+SSSP+GFH+DVEI+S ALLRSEEYR
Sbjct: 18  PRDKLPVNLSPDSASCYPPESSSSSADRISDTNESSSSPDGFHEDVEIQSSALLRSEEYR 77

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           Q FRLP DEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITAVR
Sbjct: 78  QFFRLPSDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITAVR 137

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           KAKTAGIFPNAIEIF GEKKYFFASFLSRDEAF LI+DGW QHAKGTE  M  QKSINES
Sbjct: 138 KAKTAGIFPNAIEIFVGEKKYFFASFLSRDEAFNLINDGWLQHAKGTEAIMTKQKSINES 197

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE GILG+E A ELD S DS DRSM+TPI +VSMVQANVEEEN    LP  PI+ IQE
Sbjct: 198 SRQEIGILGVEKAKELDPS-DSSDRSMSTPILNVSMVQANVEEEN---ALPAEPIDAIQE 257

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           +EPILD+H S+SRKTLTWK E+ DAPKVPD +T+VAESKF I+VEDFFSFYFSD+A++F+
Sbjct: 258 SEPILDTHGSTSRKTLTWKPEDTDAPKVPDYYTQVAESKFLIKVEDFFSFYFSDNAVDFV 317

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+FGHTR +SF+HPIK+YFGAK+ GC EIQKFRVYR   D
Sbjct: 318 SSYHEKCGDKEFKCSLWRHDDMFGHTRDISFQHPIKIYFGAKYGGCLEIQKFRVYR---D 377

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDA-DESNKCCILRVYVNVAFLKRTVWKGKI 420
           +HLVIE+ QEV+EVPYSDYFRVE +WEVKKD  DESN CCILRVYVNVAF KRTVWKGKI
Sbjct: 378 SHLVIEVTQEVSEVPYSDYFRVEAHWEVKKDVDDESNNCCILRVYVNVAFSKRTVWKGKI 437

Query: 421 VQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLER 480
           VQST+EECREAYGMW+QMA+ELLKQKLK  EEG+ SGSTS SGKDHHIEEE NNSKSLE+
Sbjct: 438 VQSTLEECREAYGMWIQMAKELLKQKLKGSEEGT-SGSTSQSGKDHHIEEEINNSKSLEK 497

Query: 481 SNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLAL 540
           SNEK D RR+TE  YSTDVDQQAEN T+GV ST+ ASW R YMKK WS+LRSQNYLPLAL
Sbjct: 498 SNEKNDQRRVTESQYSTDVDQQAENLTQGVDSTSAASWLRVYMKKLWSVLRSQNYLPLAL 557

Query: 541 VITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKD 600
           VITFAVIFLMQLSIV+LLSRPQHIHVSS +Y   +GLKFSGGRSSD  AWLEKRMHHLKD
Sbjct: 558 VITFAVIFLMQLSIVLLLSRPQHIHVSSPDY--GRGLKFSGGRSSDATAWLEKRMHHLKD 617

Query: 601 EMYMIEARLEMMRREHAHLKAQLRDFEE 628
           EMYM+EARLEMMRREHA LKAQL + EE
Sbjct: 618 EMYMVEARLEMMRREHAQLKAQLTELEE 635

BLAST of MS010316 vs. NCBI nr
Match: XP_038874406.1 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X3 [Benincasa hispida])

HSP 1 Score: 967.2 bits (2499), Expect = 6.7e-278
Identity = 504/628 (80.25%), Postives = 551/628 (87.74%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLP+  SPDSAS Y PESSSS A RISD +E+SSSP+GFH+DVEI+S ALLRSEEYR
Sbjct: 18  PRDKLPVNLSPDSASCYPPESSSSSADRISDTNESSSSPDGFHEDVEIQSSALLRSEEYR 77

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           Q FRLP DEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITAVR
Sbjct: 78  QFFRLPSDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITAVR 137

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           KAKTAGIFPNAIEIF GEKKYFFASFLSRDEAF LI+DGW QHAKGTE  M  QKSINES
Sbjct: 138 KAKTAGIFPNAIEIFVGEKKYFFASFLSRDEAFNLINDGWLQHAKGTEAIMTKQKSINES 197

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE GILG+E A ELD S DS DRSM+TPI +VSMVQANVEEEN    LP  PI+ IQE
Sbjct: 198 SRQEIGILGVEKAKELDPS-DSSDRSMSTPILNVSMVQANVEEEN---ALPAEPIDAIQE 257

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           +EPILD+H S+SRKTLTWK E+ DAPKVPD +T+VAESKF I+VEDFFSFYFSD+A++F+
Sbjct: 258 SEPILDTHGSTSRKTLTWKPEDTDAPKVPDYYTQVAESKFLIKVEDFFSFYFSDNAVDFV 317

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+FGHTR +SF+HPIK+YFGAK+ GC EIQKFRVYR   D
Sbjct: 318 SSYHEKCGDKEFKCSLWRHDDMFGHTRDISFQHPIKIYFGAKYGGCLEIQKFRVYR---D 377

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDA-DESNKCCILRVYVNVAFLKRTVWKGKI 420
           +HLVIE+ QEV+EVPYSDYFRVE +WEVKKD  DESN CCILRVYVNVAF KRTVWKGKI
Sbjct: 378 SHLVIEVTQEVSEVPYSDYFRVEAHWEVKKDVDDESNNCCILRVYVNVAFSKRTVWKGKI 437

Query: 421 VQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLER 480
           VQST+EECREAYGMW+QMA+ELLKQKLKE      SGSTS SGKDHHIEEE NNSKSLE+
Sbjct: 438 VQSTLEECREAYGMWIQMAKELLKQKLKE----GTSGSTSQSGKDHHIEEEINNSKSLEK 497

Query: 481 SNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLAL 540
           SNEK D RR+TE  YSTDVDQQAEN T+GV ST+ ASW R YMKK WS+LRSQNYLPLAL
Sbjct: 498 SNEKNDQRRVTESQYSTDVDQQAENLTQGVDSTSAASWLRVYMKKLWSVLRSQNYLPLAL 557

Query: 541 VITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKD 600
           VITFAVIFLMQLSIV+LLSRPQHIHVSS +Y   +GLKFSGGRSSD  AWLEKRMHHLKD
Sbjct: 558 VITFAVIFLMQLSIVLLLSRPQHIHVSSPDY--GRGLKFSGGRSSDATAWLEKRMHHLKD 617

Query: 601 EMYMIEARLEMMRREHAHLKAQLRDFEE 628
           EMYM+EARLEMMRREHA LKAQL + EE
Sbjct: 618 EMYMVEARLEMMRREHAQLKAQLTELEE 632

BLAST of MS010316 vs. NCBI nr
Match: XP_038874398.1 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 964.9 bits (2493), Expect = 3.3e-277
Identity = 505/628 (80.41%), Postives = 553/628 (88.06%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLP+  SPDSAS Y PESSSS A RISD +E+SSSP+GFH+DVEI+S ALLRSEEYR
Sbjct: 18  PRDKLPVNLSPDSASCYPPESSSSSADRISDTNESSSSPDGFHEDVEIQSSALLRSEEYR 77

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           Q FRLP DEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITAVR
Sbjct: 78  QFFRLPSDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITAVR 137

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           KAKTAGIFPNAIEIF GEKKYFFASFLSRDEAF LI+DGW QHAKGTE  M  QKSINES
Sbjct: 138 KAKTAGIFPNAIEIFVGEKKYFFASFLSRDEAFNLINDGWLQHAKGTEAIMTKQKSINES 197

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE GILG+E A ELD S DS D SM+TPI +VSMVQANVEEEN    LP  PI+ IQE
Sbjct: 198 SRQEIGILGVEKAKELDPS-DSSD-SMSTPILNVSMVQANVEEEN---ALPAEPIDAIQE 257

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           +EPILD+H S+SRKTLTWK E+ DAPKVPD +T+VAESKF I+VEDFFSFYFSD+A++F+
Sbjct: 258 SEPILDTHGSTSRKTLTWKPEDTDAPKVPDYYTQVAESKFLIKVEDFFSFYFSDNAVDFV 317

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+FGHTR +SF+HPIK+YFGAK+ GC EIQKFRVYR   D
Sbjct: 318 SSYHEKCGDKEFKCSLWRHDDMFGHTRDISFQHPIKIYFGAKYGGCLEIQKFRVYR---D 377

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDA-DESNKCCILRVYVNVAFLKRTVWKGKI 420
           +HLVIE+ QEV+EVPYSDYFRVE +WEVKKD  DESN CCILRVYVNVAF KRTVWKGKI
Sbjct: 378 SHLVIEVTQEVSEVPYSDYFRVEAHWEVKKDVDDESNNCCILRVYVNVAFSKRTVWKGKI 437

Query: 421 VQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLER 480
           VQST+EECREAYGMW+QMA+ELLKQKLK  EEG+ SGSTS SGKDHHIEEE NNSKSLE+
Sbjct: 438 VQSTLEECREAYGMWIQMAKELLKQKLKGSEEGT-SGSTSQSGKDHHIEEEINNSKSLEK 497

Query: 481 SNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLAL 540
           SNEK D RR+TE  YSTDVDQQAEN T+GV ST+ ASW R YMKK WS+LRSQNYLPLAL
Sbjct: 498 SNEKNDQRRVTESQYSTDVDQQAENLTQGVDSTSAASWLRVYMKKLWSVLRSQNYLPLAL 557

Query: 541 VITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKD 600
           VITFAVIFLMQLSIV+LLSRPQHIHVSS +Y   +GLKFSGGRSSD  AWLEKRMHHLKD
Sbjct: 558 VITFAVIFLMQLSIVLLLSRPQHIHVSSPDY--GRGLKFSGGRSSDATAWLEKRMHHLKD 617

Query: 601 EMYMIEARLEMMRREHAHLKAQLRDFEE 628
           EMYM+EARLEMMRREHA LKAQL + EE
Sbjct: 618 EMYMVEARLEMMRREHAQLKAQLTELEE 634

BLAST of MS010316 vs. NCBI nr
Match: XP_011650617.2 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 953.7 bits (2464), Expect = 7.7e-274
Identity = 496/628 (78.98%), Postives = 543/628 (86.46%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLP  PS DSAS Y PESSSS A RISD +E+SSSP+GF +DVEI+S ALLRSEEYR
Sbjct: 18  PRDKLPTNPSLDSASCYPPESSSSSADRISDTNESSSSPDGFQEDVEIQSSALLRSEEYR 77

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           QLFRLP DEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IP +EITAVR
Sbjct: 78  QLFRLPLDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPFREITAVR 137

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           KAKTAGIFPNAIEI  GEKKYFFASFLSRDEAF LI+DGW QHAKGTE  M+ QKSINES
Sbjct: 138 KAKTAGIFPNAIEICVGEKKYFFASFLSRDEAFNLINDGWLQHAKGTEAIMKKQKSINES 197

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE GILG+E A ELD S DS DRSM+TPI +VS+VQANVEE N P TLP  PI   QE
Sbjct: 198 SRQEIGILGVEKAKELDPS-DSSDRSMDTPILNVSVVQANVEEVNVPTTLPPEPIVATQE 257

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           AEPILD HAS+SR+TL WK E+ DAP VPD +T+VAESKFPI VEDFFSFYFSD A++F+
Sbjct: 258 AEPILDIHASTSRETLMWKPEDTDAPNVPDYYTQVAESKFPINVEDFFSFYFSDSAVDFV 317

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKE KCS WR DD+FGHTR +SF+HPIK+YFGAKF GC E QKFRVYRDRFD
Sbjct: 318 SSYHEKCGDKELKCSLWRHDDMFGHTRDVSFQHPIKIYFGAKFGGCLETQKFRVYRDRFD 377

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDA-DESNKCCILRVYVNVAFLKRTVWKGKI 420
           NHLVIE+ QEV+EVPYSDYFRVE +WEVKKD  DESN CCILRVYVNVAF KRTVWKGKI
Sbjct: 378 NHLVIEVTQEVSEVPYSDYFRVEAHWEVKKDVDDESNNCCILRVYVNVAFSKRTVWKGKI 437

Query: 421 VQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLER 480
           VQST+EECREAYG+W+QMA+ELLKQKL   EEG+  GS S SG+DHHIE+E NN+KSLE 
Sbjct: 438 VQSTLEECREAYGLWIQMAKELLKQKLTGSEEGT-RGSASQSGEDHHIEKERNNNKSLEM 497

Query: 481 SNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLAL 540
           SNEK D RR TE  YSTDVDQQA+N  +GV+ST+ ASW R Y KK W +LRSQNYLPLAL
Sbjct: 498 SNEKNDERRSTESQYSTDVDQQAKNSIQGVNSTSAASWLREYTKKLWIVLRSQNYLPLAL 557

Query: 541 VITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKD 600
           VITFAVIFLMQLSIVMLLSRPQHIHVSS +Y   +GLKFSGGRSSD  AWLEKRMHHLKD
Sbjct: 558 VITFAVIFLMQLSIVMLLSRPQHIHVSSPDY--GRGLKFSGGRSSDATAWLEKRMHHLKD 617

Query: 601 EMYMIEARLEMMRREHAHLKAQLRDFEE 628
           EMYM+EARLEMMRREHA LKAQLR+ EE
Sbjct: 618 EMYMVEARLEMMRREHAQLKAQLRELEE 641

BLAST of MS010316 vs. ExPASy Swiss-Prot
Match: F4HVW5 (Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VAD1 PE=1 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 3.7e-138
Identity = 295/634 (46.53%), Postives = 398/634 (62.78%), Query Frame = 0

Query: 4   KLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLF 63
           K+    SP+  S   P S      R  DPS +S SP+   +  + +S  + +SEEYRQLF
Sbjct: 21  KVDSSASPEVVSDLPPSSPKGSPDR-HDPSTSSPSPS---RGGDNQSEVISKSEEYRQLF 80

Query: 64  RLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAK 123
           RLP DE+L++DFNCA QE+IL+QGHMYLF+HYICFYSNIFG+ETKK+IP  EI+ V++AK
Sbjct: 81  RLPADEILVQDFNCACQESILMQGHMYLFIHYICFYSNIFGYETKKIIPFAEISCVKRAK 140

Query: 124 TAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQ 183
           TAGIFPNAIEI  G KKYFFASFLSRDEAFKLI DGW ++  G+    E +  + E  + 
Sbjct: 141 TAGIFPNAIEILAGGKKYFFASFLSRDEAFKLIHDGWLEY--GSAVKSEGEILVTE-PQV 200

Query: 184 EDGIL-----GIENATELDSST--DSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPIN 243
            DG++      ++ A ELD     ++   S ++ +P +S        +NG     V P +
Sbjct: 201 SDGVVKRARSSMDLANELDIPVRDETLHLSSSSSLPVIS--------QNG-----VPPSS 260

Query: 244 VIQEAEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDA 303
           V + AEP +D  A++   T  WK E+ DAPK+   FTKVAE+KF I VE+FF  +FSD A
Sbjct: 261 VQRHAEPDVDVVAAN---TFNWKPEDTDAPKLSSDFTKVAEAKFSIPVEEFFRLFFSDGA 320

Query: 304 INFLSSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR 363
           ++F+ S+H+ CGDKEF+C+ W+P +  GHTR++SF+HPIK+YFGAKF GC E QKFR+YR
Sbjct: 321 VSFVESFHKNCGDKEFRCTSWQPHEKLGHTRNVSFQHPIKIYFGAKFGGCQESQKFRMYR 380

Query: 364 DRFDNHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWK 423
              ++HLVIE  QE+++VPY+DYF VEG W++K+D  +S + CIL VYVNVAF KRTVWK
Sbjct: 381 ---NSHLVIETSQEISDVPYADYFTVEGVWDLKRDCRDSVEGCILDVYVNVAFSKRTVWK 440

Query: 424 GKIVQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKS 483
           GKIVQST+EECREAY  W++MA ELLKQK  E +EG+                       
Sbjct: 441 GKIVQSTLEECREAYAHWIRMAHELLKQKKLENQEGN----------------------K 500

Query: 484 LERSNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFW----SLLRSQ 543
           L    E + AR   E     D + + E    GV   +        +K+ W    S ++ Q
Sbjct: 501 LIEDGEPLAARE--ERVSECDEEGKVEMVGEGVVKKS--------LKEAWVNLTSFVKRQ 560

Query: 544 NYLPLALVITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEK 603
           +     +V+ FAVI LMQ++IV+LL +     V   E  +   +      + + + WLEK
Sbjct: 561 SGTRQVIVLAFAVILLMQVTIVVLLKKGGGGQVEYHERYDEYSV------NGETLGWLEK 590

Query: 604 RMHHLKDEMYMIEARLEMMRREHAHLKAQLRDFE 627
           RMH L++EM M+E RL+ MR++HA LKAQ    E
Sbjct: 621 RMHFLREEMMMVEDRLQRMRQDHAALKAQFHHLE 590

BLAST of MS010316 vs. ExPASy Swiss-Prot
Match: Q96CP6 (Protein Aster-A OS=Homo sapiens OX=9606 GN=GRAMD1A PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 3.0e-23
Identity = 179/676 (26.48%), Postives = 264/676 (39.05%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQD-------VEIESPAL 60
           P  + P EP P +      +SSS   G    PS  S     F ++         + SP  
Sbjct: 27  PPSRPPPEPEPGTMVEKGSDSSSEKGGVPGTPSTQSLGSRNFIRNSKKMQSWYSMLSPTY 86

Query: 61  -LRSEEYRQLF-RLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVI 120
             R+E++R+LF +LP  E LI D++CA Q  IL+QG +YL  ++ICFYSNIF +ET   I
Sbjct: 87  KQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTISI 146

Query: 121 PLQEITAVRKAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWS---------- 180
            L+E+T ++K KTA + PNAI+I    +K+FF SF +RD  F LI   W           
Sbjct: 147 QLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLWQNALLEKTLSP 206

Query: 181 -------QHAKGTE---------------------------------------------- 240
                      G+E                                              
Sbjct: 207 RELWHLVHQCYGSELGLTSEDEDYVSPLQLNGLGTPKEVGDVIALSDITSSGAADRSQEP 266

Query: 241 ---GNMENQKSINESSRQEDGILGIENATE--LDSSTDSFDRSMNTPIPDVSMVQANVEE 300
              G+     + N S    D   G E   E  +DS  D+      TP+ +    +    +
Sbjct: 267 SPVGSRRGHVTPNLSRASSDADHGAEEDKEEQVDSQPDASSSQTVTPVAEPPSTEPT--Q 326

Query: 301 ENGPKTLPVVPINVIQEAEPILDSHASSSRKTLTWKLENVD-APKVPDCFTK-VAESKFP 360
            +GP TL   P++++   E + D+  SSS        E  D A  +PD   + +  S F 
Sbjct: 327 PDGPTTLG--PLDLLPSEELLTDTSNSSSSTG-----EEADLAALLPDLSGRLLINSVFH 386

Query: 361 IQVEDFFSFYFSDDAINFLSSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGA 420
           +  E      FSD    FL  + +QC   +   SPW  D      R L++  PI    G 
Sbjct: 387 VGAERLQQMLFSDSP--FLQGFLQQCKFTDVTLSPWSGDSKCHQRRVLTYTIPISNPLGP 446

Query: 421 KFSGCSEIQK-FRVYRDRFDNHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCI 480
           K +   E Q  FR  R       V++       +PY DYF              +++ CI
Sbjct: 447 KSASVVETQTLFR--RGPQAGGCVVDSEVLTQGIPYQDYFYT------------AHRYCI 506

Query: 481 LRVYVNVAFL--------KRTVWKGKIVQSTIEECR----EAYGMWVQMARELLKQKLKE 540
           L +  N A L        ++  W   +V+S IE+      E Y  +  + REL K +   
Sbjct: 507 LGLARNKARLRVSSEIRYRKQPW--SLVKSLIEKNSWSGIEDY--FHHLERELAKAEKLS 566

Query: 541 FEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDARRLTEMPYSTDVD--QQAENFT 576
            EEG        SG              L R    +  R   + P   D D   +A   T
Sbjct: 567 LEEGGKDARGLLSG--------------LRRRKRPLSWRAHGDGPQHPDPDPCARAGIHT 626

BLAST of MS010316 vs. ExPASy Swiss-Prot
Match: Q06681 (Membrane-anchored lipid-binding protein YSP2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YSP2 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 6.3e-21
Identity = 107/439 (24.37%), Postives = 192/439 (43.74%), Query Frame = 0

Query: 19   PESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFR---LPPDEVLIEDF 78
            P +S++ +G I       S  + F  +  IE  +  ++ E+  LF+   + P+E LI D 
Sbjct: 610  PRTSTAGSGNIHAIMGLDSGNDDFKLE-GIEYASEKKNSEFHTLFKDCDINPNEKLIVDH 669

Query: 79   NCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAGIFPNAIEIF 138
            +CA   +IL+QG MY+   +I F+SNI G+ +   IP +EI  + K  TAGIFPN I I 
Sbjct: 670  SCALSRDILLQGRMYISDAHIGFFSNILGWVSTVFIPFKEIVQIEKKTTAGIFPNGIVID 729

Query: 139  DGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDGILGIENATE 198
                KY FASF+SRD  F LI D W+Q   G       +K  N     +DG +   ++  
Sbjct: 730  TLHTKYIFASFMSRDATFDLITDVWNQIILG-------KKYRNGFGNNDDGTISDSSSAF 789

Query: 199  LDSSTDS-FDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILDSHASSSRK 258
             D S D+  D  ++   PD++       ++        +  +V  E+  +       ++K
Sbjct: 790  FDDSDDNDDDGDLDDDDPDINSTDMTSSDD--------IDADVFNESNDL-----GKNQK 849

Query: 259  TLTWKL-ENVDAPKVPDCFTK-----VAESKFPIQVEDFFSFYFSDDAINFLSSYHEQC- 318
            +  + L  N  +P   D         V E+     +    +  + +D      SY+E+  
Sbjct: 850  STNYLLGPNKHSPTTADFKPSNNDHLVIEANINAPLGKVVNLLYGEDV-----SYYERIL 909

Query: 319  -GDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFDNHLVIE 378
               K F+ SP   + +    R  ++  P+    G   + C        Y    ++++ + 
Sbjct: 910  KAQKNFEISPIPNNFLTKKIRDYAYTKPLSGSIGPSKTKCLITDTLEHY--DLEDYVKVL 969

Query: 379  LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE 438
             + +  +VP  + F V+  +    D + S K   L VY +V +  ++  K  I + T + 
Sbjct: 970  SITKNPDVPSGNIFSVKTVFLFSWDKNNSTK---LTVYNSVDWTGKSWIKSMIEKGTFDG 1017

Query: 439  CREAYGMWVQMARELLKQK 446
              +   + +   +++L  +
Sbjct: 1030 VADTTKIMISEIKKILSDE 1017

BLAST of MS010316 vs. ExPASy Swiss-Prot
Match: Q8CI52 (Protein Aster-C OS=Mus musculus OX=10090 GN=Gramd1c PE=1 SV=2)

HSP 1 Score: 102.1 bits (253), Expect = 2.4e-20
Identity = 53/107 (49.53%), Postives = 72/107 (67.29%), Query Frame = 0

Query: 55  RSEEYRQLF-RLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPL 114
           R+EEYRQ F  LP  E LI D+ CA Q++IL+QG +YL   ++CFYSNIF +ET   I L
Sbjct: 67  RNEEYRQQFTHLPDSEKLIADYACALQKDILVQGRLYLSEKWLCFYSNIFRWETTISIAL 126

Query: 115 QEITAVRKAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGW 161
           + IT + K KTA + PNAI+I    +K+FF SF +RD ++ +I   W
Sbjct: 127 KNITFMTKEKTARLIPNAIQIITEGEKFFFTSFGARDRSYLIIFRLW 173

BLAST of MS010316 vs. ExPASy Swiss-Prot
Match: Q3KR56 (Protein Aster-A OS=Rattus norvegicus OX=10116 GN=Gramd1a PE=2 SV=2)

HSP 1 Score: 99.8 bits (247), Expect = 1.2e-19
Identity = 68/171 (39.77%), Postives = 97/171 (56.73%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPES-SSSPAGRISDPSETSS--SPNGFHQDVEIESPALL--- 60
           P  + P  P P+  +     S SSS    +S    T S  S N      +++S   +   
Sbjct: 27  PPSRPPSAPEPEPGTMVEKGSDSSSEKSGVSGTLSTQSLGSRNFIRNSKKMQSWYSMLCP 86

Query: 61  ----RSEEYRQLF-RLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKK 120
               R+E++R+LF +LP  E LI D++CA Q  IL+QG +YL  ++ICFYSNIF +ET  
Sbjct: 87  TYKQRNEDFRKLFSKLPEAERLIVDYSCALQREILLQGRLYLSENWICFYSNIFRWETTI 146

Query: 121 VIPLQEITAVRKAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGW 161
            I L+E+T ++K KTA + PNAI+I    +K+FF SF +RD  F LI   W
Sbjct: 147 SIQLKEVTCLKKEKTAKLIPNAIQICTESEKHFFTSFGARDRCFLLIFRLW 197

BLAST of MS010316 vs. ExPASy TrEMBL
Match: A0A6J1CTM4 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014217 PE=4 SV=1)

HSP 1 Score: 1223.8 bits (3165), Expect = 0.0e+00
Identity = 617/621 (99.36%), Postives = 618/621 (99.52%), Query Frame = 0

Query: 7   MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP 66
           MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP
Sbjct: 1   MEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLFRLP 60

Query: 67  PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG 126
           PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG
Sbjct: 61  PDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAKTAG 120

Query: 127 IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG 186
           IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG
Sbjct: 121 IFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQEDG 180

Query: 187 ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD 246
           ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD
Sbjct: 181 ILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQEAEPILD 240

Query: 247 SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ 306
           SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ
Sbjct: 241 SHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFLSSYHEQ 300

Query: 307 CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFDNHLVIE 366
           CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR   D+HLVIE
Sbjct: 301 CGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR---DSHLVIE 360

Query: 367 LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE 426
           LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE
Sbjct: 361 LMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIVQSTIEE 420

Query: 427 CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA 486
           CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA
Sbjct: 421 CREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERSNEKIDA 480

Query: 487 RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI 546
           RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI
Sbjct: 481 RRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALVITFAVI 540

Query: 547 FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA 606
           FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA
Sbjct: 541 FLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDEMYMIEA 600

Query: 607 RLEMMRREHAHLKAQLRDFEE 628
           RLEMMRREHAHLKAQLRDFEE
Sbjct: 601 RLEMMRREHAHLKAQLRDFEE 618

BLAST of MS010316 vs. ExPASy TrEMBL
Match: A0A0A0L3J6 (VASt domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116750 PE=4 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 4.7e-269
Identity = 491/628 (78.18%), Postives = 539/628 (85.83%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLP  PS DSAS Y PESSSS A RISD +E+SSSP+GF +DVEI+S ALLRSEEYR
Sbjct: 18  PRDKLPTNPSLDSASCYPPESSSSSADRISDTNESSSSPDGFQEDVEIQSSALLRSEEYR 77

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           QL RLP DEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IP +EITAVR
Sbjct: 78  QLSRLPLDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPFREITAVR 137

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           KAKTAGIFPNAIEI  GEKKYFFASFLSRDEAF LI+DGW QHAKGTE  M+ QKSINES
Sbjct: 138 KAKTAGIFPNAIEICVGEKKYFFASFLSRDEAFNLINDGWLQHAKGTEAIMKKQKSINES 197

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE GILG+E A ELD S DS DRSM+TPI +VS+VQANVEE N P TLP  PI   QE
Sbjct: 198 SRQEIGILGVEKAKELDPS-DSSDRSMDTPILNVSVVQANVEEVNVPTTLPPEPIVATQE 257

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           AEPILD HAS+SR+TL WK E+ DAP VPD +T+VAESKFPI VEDFFSFYFSD A++F+
Sbjct: 258 AEPILDIHASTSRETLMWKPEDTDAPNVPDYYTQVAESKFPINVEDFFSFYFSDSAVDFV 317

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKE KCS WR DD+FGHTR +SF+HPIK+YFGAKF GC E QKFRVYR   D
Sbjct: 318 SSYHEKCGDKELKCSLWRHDDMFGHTRDVSFQHPIKIYFGAKFGGCLETQKFRVYR---D 377

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDA-DESNKCCILRVYVNVAFLKRTVWKGKI 420
           +HLVIE+ QEV+EVPYSDYFRVE +WEVKKD  DESN CCILRVYVNVAF KRTVWKGKI
Sbjct: 378 SHLVIEVTQEVSEVPYSDYFRVEAHWEVKKDVDDESNNCCILRVYVNVAFSKRTVWKGKI 437

Query: 421 VQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLER 480
           VQST+EECREAYG+W+QMA+ELLKQKL   EEG+  GS S SG+DHHIE+E NN+KSLE 
Sbjct: 438 VQSTLEECREAYGLWIQMAKELLKQKLTGSEEGT-RGSASQSGEDHHIEKERNNNKSLEM 497

Query: 481 SNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLAL 540
           SNEK D RR TE  YSTDVDQQA+N  +GV+ST+ ASW R Y KK W +LRSQNYLPLAL
Sbjct: 498 SNEKNDERRSTESQYSTDVDQQAKNSIQGVNSTSAASWLREYTKKLWIVLRSQNYLPLAL 557

Query: 541 VITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKD 600
           VITFAVIFLMQLSIVMLLSRPQHIHVSS +Y   +GLKFSGGRSSD  AWLEKRMHHLKD
Sbjct: 558 VITFAVIFLMQLSIVMLLSRPQHIHVSSPDY--GRGLKFSGGRSSDATAWLEKRMHHLKD 617

Query: 601 EMYMIEARLEMMRREHAHLKAQLRDFEE 628
           EMYM+EARLEMMRREHA LKAQLR+ EE
Sbjct: 618 EMYMVEARLEMMRREHAQLKAQLRELEE 638

BLAST of MS010316 vs. ExPASy TrEMBL
Match: A0A6J1F4N9 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442129 PE=4 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 2.3e-268
Identity = 484/627 (77.19%), Postives = 540/627 (86.12%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PR KLPM+ SPDSAS Y P+SSSS A R +DP+ETSS P+G H+D+EI+S ALLRSEEYR
Sbjct: 14  PRHKLPMDLSPDSASCYPPQSSSSSAHRTADPNETSSPPDGLHEDLEIQSSALLRSEEYR 73

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           QLFRLPPDEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITA+R
Sbjct: 74  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITALR 133

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           +AKTAGIFPNAIEI  GEKK+FFASFLSRDEAF LI+DGWSQ  KGTE  +  Q S+NES
Sbjct: 134 RAKTAGIFPNAIEIIVGEKKFFFASFLSRDEAFNLINDGWSQQGKGTEAILTKQISVNES 193

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE   LGIE A ELD S DS DRSMNTPI +VSMVQANVEEENG KTLP VPI+VIQE
Sbjct: 194 SRQEISNLGIEKAKELDPS-DSSDRSMNTPILNVSMVQANVEEENGTKTLPPVPIDVIQE 253

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           AEPILD+HAS+SRK + WK E+ +APKVPDC+TKVAESKFPI+VEDFFSFYFSDDA++F+
Sbjct: 254 AEPILDAHASTSRK-MMWKQEDTEAPKVPDCYTKVAESKFPIKVEDFFSFYFSDDAVDFV 313

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+ G TR +SF+HPIK+YFGAKF GC EIQKFRVYR   D
Sbjct: 314 SSYHEKCGDKEFKCSLWRTDDVLGPTRDISFQHPIKIYFGAKFGGCQEIQKFRVYR---D 373

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIV 420
           +HLV+E++QEV++VPYSDYFRVE +WEVK D DESN  CIL VYVNVAF KRTVWKGKIV
Sbjct: 374 SHLVVEVIQEVSDVPYSDYFRVEVHWEVKNDVDESNNGCILNVYVNVAFSKRTVWKGKIV 433

Query: 421 QSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERS 480
           QST+EECREAYG+W+QMA+ELL+QKLKE EEG P GSTS S KD  +EEE N SKSLE+S
Sbjct: 434 QSTLEECREAYGLWIQMAKELLQQKLKEIEEGRPRGSTSGSSKDPPVEEEINRSKSLEKS 493

Query: 481 NEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALV 540
           NE  D RRLTE  +STDVDQQ EN ++GV S + ASW R Y+KK  S LRSQNYLPLALV
Sbjct: 494 NEMNDQRRLTESQFSTDVDQQPENLSQGVDSPSHASWWREYVKKLCSSLRSQNYLPLALV 553

Query: 541 ITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDE 600
           ITF VIFLMQLSIV+LLSRPQHIH+S  +  NA GLKFSGGRS D MAWLEKRMHHLKDE
Sbjct: 554 ITFVVIFLMQLSIVVLLSRPQHIHMSYPD--NASGLKFSGGRSPDTMAWLEKRMHHLKDE 613

Query: 601 MYMIEARLEMMRREHAHLKAQLRDFEE 628
           M M+EARLEMMRREHA LKAQLRD EE
Sbjct: 614 MNMVEARLEMMRREHAQLKAQLRDLEE 633

BLAST of MS010316 vs. ExPASy TrEMBL
Match: A0A6J1IFM2 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472873 PE=4 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.6e-267
Identity = 485/627 (77.35%), Postives = 538/627 (85.81%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PRDKLPM+ SPDSAS Y P+SSSS A R +DP+ETSS P+GFH+DVEI+S ALLRSEEYR
Sbjct: 14  PRDKLPMDLSPDSASCYPPQSSSSSADRTADPNETSSPPDGFHEDVEIQSSALLRSEEYR 73

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           QLFRLPPDEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITA+R
Sbjct: 74  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITALR 133

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           +AKTAGIFPNAIEI  GEKK+FFASFLSRDEAF LI+DGWSQ  KGTE  +  Q S+NES
Sbjct: 134 RAKTAGIFPNAIEILVGEKKFFFASFLSRDEAFNLINDGWSQQGKGTEAILTKQISVNES 193

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE   LGIE A ELD S DS DRSMNTPI +VSMVQANVEEENGPKTLP VPI+ IQE
Sbjct: 194 SRQEISNLGIEKAKELDPS-DSSDRSMNTPILNVSMVQANVEEENGPKTLPPVPIDTIQE 253

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           AEPILD+HAS+ RK + WK E+ +APKVPDC+TKVAESKFPI+VEDFFSFYFSDDA++F+
Sbjct: 254 AEPILDAHASTLRK-MMWKQEDTEAPKVPDCYTKVAESKFPIKVEDFFSFYFSDDAVDFV 313

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+ G TR +SF+HPIK+YFGAKF GC EIQKFRVYR   D
Sbjct: 314 SSYHEKCGDKEFKCSLWRTDDVLGPTRDISFQHPIKIYFGAKFGGCQEIQKFRVYR---D 373

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIV 420
           +HLVIE++QEV++VPYSDYFRVE +WEVK D DESN  CIL VYVNVAF KRTVWKGKIV
Sbjct: 374 SHLVIEVIQEVSDVPYSDYFRVEVHWEVKNDVDESNNGCILNVYVNVAFSKRTVWKGKIV 433

Query: 421 QSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERS 480
           QST+EECREAYG+W+QMA+ELL+QKLKE EEG P GSTS S KD  +EEE N SKSLE+S
Sbjct: 434 QSTLEECREAYGLWIQMAKELLQQKLKEIEEGRPRGSTSGSCKDPPVEEEINRSKSLEKS 493

Query: 481 NEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALV 540
           NE  D RRLTE   STDV QQ E  ++GV S + ASW R Y+KK  SLLRSQNYLPLALV
Sbjct: 494 NETNDQRRLTESQCSTDVGQQPEILSQGVDSPSHASWWREYVKKLCSLLRSQNYLPLALV 553

Query: 541 ITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDE 600
           ITF VIFLMQLSIV+LLSRPQHIH+S  +  NA GLK SGGRS D MAWLEKRMHHLKDE
Sbjct: 554 ITFVVIFLMQLSIVVLLSRPQHIHMSYPD--NASGLKVSGGRSPDTMAWLEKRMHHLKDE 613

Query: 601 MYMIEARLEMMRREHAHLKAQLRDFEE 628
           M M+EARLEMMRREHA LKAQLRD EE
Sbjct: 614 MNMVEARLEMMRREHAQLKAQLRDLEE 633

BLAST of MS010316 vs. ExPASy TrEMBL
Match: A0A6J1F3U9 (protein VASCULAR ASSOCIATED DEATH 1, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442129 PE=4 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.2e-266
Identity = 483/627 (77.03%), Postives = 539/627 (85.96%), Query Frame = 0

Query: 1   PRDKLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYR 60
           PR KLPM+ SPDSAS Y P+SSSS A R +DP+ETSS P+G H+D+EI+S ALLRSEEYR
Sbjct: 14  PRHKLPMDLSPDSASCYPPQSSSSSAHRTADPNETSSPPDGLHEDLEIQSSALLRSEEYR 73

Query: 61  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVR 120
           QLFRLPPDEVLIEDFNCAFQENILIQGHMYLF+HYICFYSNIFGFETKK+IPLQEITA+R
Sbjct: 74  QLFRLPPDEVLIEDFNCAFQENILIQGHMYLFVHYICFYSNIFGFETKKIIPLQEITALR 133

Query: 121 KAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINES 180
           +AKTAGIFPNAIEI  GEKK+FFASFLSRDEAF LI+DGWSQ  KGTE  +  Q S+NES
Sbjct: 134 RAKTAGIFPNAIEIIVGEKKFFFASFLSRDEAFNLINDGWSQQGKGTEAILTKQISVNES 193

Query: 181 SRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPINVIQE 240
           SRQE   LGIE A ELD S DS D SMNTPI +VSMVQANVEEENG KTLP VPI+VIQE
Sbjct: 194 SRQEISNLGIEKAKELDPS-DSSD-SMNTPILNVSMVQANVEEENGTKTLPPVPIDVIQE 253

Query: 241 AEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDAINFL 300
           AEPILD+HAS+SRK + WK E+ +APKVPDC+TKVAESKFPI+VEDFFSFYFSDDA++F+
Sbjct: 254 AEPILDAHASTSRK-MMWKQEDTEAPKVPDCYTKVAESKFPIKVEDFFSFYFSDDAVDFV 313

Query: 301 SSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYRDRFD 360
           SSYHE+CGDKEFKCS WR DD+ G TR +SF+HPIK+YFGAKF GC EIQKFRVYR   D
Sbjct: 314 SSYHEKCGDKEFKCSLWRTDDVLGPTRDISFQHPIKIYFGAKFGGCQEIQKFRVYR---D 373

Query: 361 NHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWKGKIV 420
           +HLV+E++QEV++VPYSDYFRVE +WEVK D DESN  CIL VYVNVAF KRTVWKGKIV
Sbjct: 374 SHLVVEVIQEVSDVPYSDYFRVEVHWEVKNDVDESNNGCILNVYVNVAFSKRTVWKGKIV 433

Query: 421 QSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKSLERS 480
           QST+EECREAYG+W+QMA+ELL+QKLKE EEG P GSTS S KD  +EEE N SKSLE+S
Sbjct: 434 QSTLEECREAYGLWIQMAKELLQQKLKEIEEGRPRGSTSGSSKDPPVEEEINRSKSLEKS 493

Query: 481 NEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFWSLLRSQNYLPLALV 540
           NE  D RRLTE  +STDVDQQ EN ++GV S + ASW R Y+KK  S LRSQNYLPLALV
Sbjct: 494 NEMNDQRRLTESQFSTDVDQQPENLSQGVDSPSHASWWREYVKKLCSSLRSQNYLPLALV 553

Query: 541 ITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEKRMHHLKDE 600
           ITF VIFLMQLSIV+LLSRPQHIH+S  +  NA GLKFSGGRS D MAWLEKRMHHLKDE
Sbjct: 554 ITFVVIFLMQLSIVVLLSRPQHIHMSYPD--NASGLKFSGGRSPDTMAWLEKRMHHLKDE 613

Query: 601 MYMIEARLEMMRREHAHLKAQLRDFEE 628
           M M+EARLEMMRREHA LKAQLRD EE
Sbjct: 614 MNMVEARLEMMRREHAQLKAQLRDLEE 632

BLAST of MS010316 vs. TAIR 10
Match: AT1G02120.1 (GRAM domain family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.6e-139
Identity = 295/634 (46.53%), Postives = 398/634 (62.78%), Query Frame = 0

Query: 4   KLPMEPSPDSASYYTPESSSSPAGRISDPSETSSSPNGFHQDVEIESPALLRSEEYRQLF 63
           K+    SP+  S   P S      R  DPS +S SP+   +  + +S  + +SEEYRQLF
Sbjct: 21  KVDSSASPEVVSDLPPSSPKGSPDR-HDPSTSSPSPS---RGGDNQSEVISKSEEYRQLF 80

Query: 64  RLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRKAK 123
           RLP DE+L++DFNCA QE+IL+QGHMYLF+HYICFYSNIFG+ETKK+IP  EI+ V++AK
Sbjct: 81  RLPADEILVQDFNCACQESILMQGHMYLFIHYICFYSNIFGYETKKIIPFAEISCVKRAK 140

Query: 124 TAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAKGTEGNMENQKSINESSRQ 183
           TAGIFPNAIEI  G KKYFFASFLSRDEAFKLI DGW ++  G+    E +  + E  + 
Sbjct: 141 TAGIFPNAIEILAGGKKYFFASFLSRDEAFKLIHDGWLEY--GSAVKSEGEILVTE-PQV 200

Query: 184 EDGIL-----GIENATELDSST--DSFDRSMNTPIPDVSMVQANVEEENGPKTLPVVPIN 243
            DG++      ++ A ELD     ++   S ++ +P +S        +NG     V P +
Sbjct: 201 SDGVVKRARSSMDLANELDIPVRDETLHLSSSSSLPVIS--------QNG-----VPPSS 260

Query: 244 VIQEAEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVEDFFSFYFSDDA 303
           V + AEP +D  A++   T  WK E+ DAPK+   FTKVAE+KF I VE+FF  +FSD A
Sbjct: 261 VQRHAEPDVDVVAAN---TFNWKPEDTDAPKLSSDFTKVAEAKFSIPVEEFFRLFFSDGA 320

Query: 304 INFLSSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFKHPIKLYFGAKFSGCSEIQKFRVYR 363
           ++F+ S+H+ CGDKEF+C+ W+P +  GHTR++SF+HPIK+YFGAKF GC E QKFR+YR
Sbjct: 321 VSFVESFHKNCGDKEFRCTSWQPHEKLGHTRNVSFQHPIKIYFGAKFGGCQESQKFRMYR 380

Query: 364 DRFDNHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVYVNVAFLKRTVWK 423
              ++HLVIE  QE+++VPY+DYF VEG W++K+D  +S + CIL VYVNVAF KRTVWK
Sbjct: 381 ---NSHLVIETSQEISDVPYADYFTVEGVWDLKRDCRDSVEGCILDVYVNVAFSKRTVWK 440

Query: 424 GKIVQSTIEECREAYGMWVQMARELLKQKLKEFEEGSPSGSTSYSGKDHHIEEETNNSKS 483
           GKIVQST+EECREAY  W++MA ELLKQK  E +EG+                       
Sbjct: 441 GKIVQSTLEECREAYAHWIRMAHELLKQKKLENQEGN----------------------K 500

Query: 484 LERSNEKIDARRLTEMPYSTDVDQQAENFTRGVHSTTFASWSRGYMKKFW----SLLRSQ 543
           L    E + AR   E     D + + E    GV   +        +K+ W    S ++ Q
Sbjct: 501 LIEDGEPLAARE--ERVSECDEEGKVEMVGEGVVKKS--------LKEAWVNLTSFVKRQ 560

Query: 544 NYLPLALVITFAVIFLMQLSIVMLLSRPQHIHVSSTEYANAKGLKFSGGRSSDGMAWLEK 603
           +     +V+ FAVI LMQ++IV+LL +     V   E  +   +      + + + WLEK
Sbjct: 561 SGTRQVIVLAFAVILLMQVTIVVLLKKGGGGQVEYHERYDEYSV------NGETLGWLEK 590

Query: 604 RMHHLKDEMYMIEARLEMMRREHAHLKAQLRDFE 627
           RMH L++EM M+E RL+ MR++HA LKAQ    E
Sbjct: 621 RMHFLREEMMMVEDRLQRMRQDHAALKAQFHHLE 590

BLAST of MS010316 vs. TAIR 10
Match: AT3G59660.1 (C2 domain-containing protein / GRAM domain-containing protein )

HSP 1 Score: 86.3 bits (212), Expect = 9.7e-17
Identity = 90/408 (22.06%), Postives = 160/408 (39.22%), Query Frame = 0

Query: 62  LFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGFETKKVIPLQEITAVRK 121
           +F L PDEV+   ++CA + + L  G MY+   +ICF+SN+F  + K V+PL +I  +R+
Sbjct: 234 IFDLLPDEVVEHSYSCALERSFLYHGRMYVSAWHICFHSNVFSKQMKVVVPLGDIDEIRR 293

Query: 122 AKTAGIFPNAIEIF----------------DGEKKYFFASFLSRDEAFKLIDDGWSQHAK 181
           ++ A I P AI I                 DG  +Y FASF +R+   K +         
Sbjct: 294 SQHALINP-AITIILRMGAGGHGVPPLGTPDGRVRYKFASFWNRNHTLKAL--------- 353

Query: 182 GTEGNMENQKSINESSRQEDGILGIENATELDSSTDSFDRSMNTPIPDVSMVQANVEEEN 241
                   Q+++N        +L +E      S+     R+ ++ +     VQ    E+ 
Sbjct: 354 --------QRAVN----NYHAMLEVEKKERAQSAL----RAHSSSVKGGGKVQVKAPEDT 413

Query: 242 GPKTLPVVPINVIQEAEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKFPIQVE 301
               +PV     I+E                             +    +    F    E
Sbjct: 414 A--AVPVKFQAFIKE-----------------------------EVLVNIYNDVFASTPE 473

Query: 302 DFFSFYFSDDAINFLSSYHEQCGDKEFKCSPWR-PDDIFGHTRHLSFKHPIKLYFGAKFS 361
              +   +DD+  + + Y     DK     PW   ++  G  R + F+           +
Sbjct: 474 QVLNVLLADDS-TYTNEYRSARKDKNLNIEPWHTAEEYDGQVREIKFRSICNSPMCPPDT 533

Query: 362 GCSEIQKFRVYRDRFDNHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADESNKCCILRVY 421
             +E Q   +  D+    LV E +Q+ ++VP+  YF V   W ++   + S+   ++ + 
Sbjct: 534 AVTEWQHVVLSPDK--KVLVFETVQQPHDVPFGSYFEVHCRWRLEAKDETSS---VIDIR 578

Query: 422 VNVAFLKRTVWKGKIVQSTIEECREAYGMWVQMARELLKQKLKEFEEG 453
           V V F K  + + KI    I+E ++   + +++A   LK        G
Sbjct: 594 VGVHFKKWCLMQSKIKAGAIDEYKKEVEVMLEVALSYLKSHSSSSSHG 578

BLAST of MS010316 vs. TAIR 10
Match: AT5G50170.1 (C2 calcium/lipid-binding and GRAM domain containing protein )

HSP 1 Score: 52.4 bits (124), Expect = 1.6e-06
Identity = 80/395 (20.25%), Postives = 158/395 (40.00%), Query Frame = 0

Query: 46   VEIESPALLRSEEYRQLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGF 105
            + I SP   ++  +++LF LP +E L++++ C  +  + +QG ++L    + FYSN+FG 
Sbjct: 683  LNIRSPQ--KNSAFQKLFGLPHEEFLLKEYTCYLKRKLPVQGKLFLSARIVAFYSNVFGH 742

Query: 106  ETKKVIPLQEITAVRKAKTAGIFPNAIEIFDGEKKYFFASFLSRDEAFKLIDDGWSQHAK 165
            +TK     ++I  ++      + P               +F S      LI         
Sbjct: 743  KTKFYFLWEDIDDIQ------VLP--------------PTFASLGSPLLLII-------- 802

Query: 166  GTEGNMENQKSINESSRQEDGILG--IENATELDSSTDSFDRSMNTPIPDVSMVQANVEE 225
              + N          S+ ++G L    ++    D+++ +      T    V      VEE
Sbjct: 803  -LKKNRGLDAKHGAKSQDDEGRLSFYFQSFVSFDATSRTIMALWKTRTLSVDHRAQIVEE 862

Query: 226  EN---GPKTLPVVPINVIQEAEPILDSHASSSRKTLTWKLENVDAPKVPDCFTKVAESKF 285
            +     P  LP   + V+ +A+ ++                           +KV     
Sbjct: 863  DQDVADPFLLPEA-VTVVSDADALM--------------------------MSKVYTCDL 922

Query: 286  PIQVEDFFSFYFSDDAINFLSSYHEQCGDKEFKCSPWRPDDIFGHTRHLSFK--HPIKLY 345
            P  VE     +   +    +    E+ G   +  + W       + R LS+K  H + ++
Sbjct: 923  PCDVELVMKIFGGGELERKIM---EKSGCLSYASTTWESKKPGVYERRLSYKYNHYVSVF 982

Query: 346  FGAKFSGCSEIQKFRVYRDRFDNHLVIELMQEVNEVPYSDYFRVEGYWEVKKDADE--SN 405
             G     C++ QK        D   ++  +  +++VP+ D+FRV   +EVKK   +  ++
Sbjct: 983  GGG--VTCAQ-QKSPAPN---DEGWILNEIVALHDVPFGDHFRVHIRYEVKKAGVDCKTS 1007

Query: 406  KCCILRVYVNVAFLKRTVWKGKIVQSTIEECREAY 432
            KC    VY+ + +LK   ++ +I +S +E+ R  +
Sbjct: 1043 KC---EVYLKIRWLKTIKFEQRISKSIMEKFRNRF 1007

BLAST of MS010316 vs. TAIR 10
Match: AT1G03370.1 (C2 calcium/lipid-binding and GRAM domain containing protein )

HSP 1 Score: 47.0 bits (110), Expect = 6.5e-05
Identity = 41/170 (24.12%), Postives = 78/170 (45.88%), Query Frame = 0

Query: 46  VEIESPALLRSEEYRQLFRLPPDEVLIEDFNCAFQENILIQGHMYLFMHYICFYSNIFGF 105
           + + SP    +  +++LF LP +E LI DF C  +  + +QG ++L    + FY++IFG 
Sbjct: 679 INVRSPQ--TNSAFQKLFGLPQEEFLINDFTCHLKRKMPLQGRLFLSARIVGFYASIFGN 738

Query: 106 ETKKVIPLQEITAVR-KAKTAGIFPNAIEIF-------------------DGEKKYFFAS 165
           +TK     ++I  ++    T     + I +                    +G  K+ F S
Sbjct: 739 KTKFFFLWEDIEEIQVLPPTLASMGSPIVVMTLRPNRGLDARIGAKTHDEEGRLKFHFHS 798

Query: 166 FLSRDEAFKLIDDGWSQHAKGTEGNME--NQKSINESSRQEDGI-LGIEN 193
           F+S + A K I   W   +   E  ++   ++S  +   +E G+ LG+++
Sbjct: 799 FVSFNVAQKTIMALWKAKSLTPEQKVQAVEEESEQKLQSEESGLFLGVDD 846