MS010288 (gene) Bitter gourd (TR) v1

BLAST of MS010288 vs. NCBI nr
Match: XP_004149661.1 (GDSL esterase/lipase At1g71250 [Cucumis sativus])

HSP 1 Score: 502.3 bits (1292), Expect = 3.5e-138
Identity = 244/338 (72.19%), Postives = 285/338 (84.32%), Query Frame = 0

Query: 27  QVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q SNV AM+LLGDSSVDCG NTL+YPLLHRNFSL PC+A+A    S+LLP  LA KIG+P
Sbjct: 25  QESNVNAMFLLGDSSVDCGFNTLFYPLLHRNFSLLPCDANA---TSSLLPFLLADKIGLP 84

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           ++QP Y QN S+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  
Sbjct: 85  HAQPFYNQNESLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHA 144

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FYLSFGE+DFINLYLL S+  + KY G+EF RLLVSQMVIAIRNL+EAGAR
Sbjct: 145 ARHFIQSSLFYLSFGEVDFINLYLLKSS--ERKYGGEEFARLLVSQMVIAIRNLQEAGAR 204

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FG 266
           KI+CMGILPLGC+PRVLS WR+SP    D+KGCVKEMNELV +YN  ME+++VK    FG
Sbjct: 205 KIVCMGILPLGCSPRVLSEWRDSPADTLDKKGCVKEMNELVGKYNEVMEEEMVKLNAEFG 264

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVW 326
             +++FCD YKGMMEI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VW
Sbjct: 265 DTQMIFCDVYKGMMEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVW 324

Query: 327 WDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLVW 360
           WDLYNPTG VN+LLADSAW ++PFS  C PST+QDL++
Sbjct: 325 WDLYNPTGVVNALLADSAWGNRPFSTICRPSTIQDLLF 357

BLAST of MS010288 vs. NCBI nr
Match: XP_008449874.1 (PREDICTED: GDSL esterase/lipase At1g71250-like isoform X2 [Cucumis melo] >KAA0040096.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 500.0 bits (1286), Expect = 1.7e-137
Identity = 243/335 (72.54%), Postives = 282/335 (84.18%), Query Frame = 0

Query: 29  SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG NTL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           LYNPTG VNSLLADSAW +QPFS  CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

BLAST of MS010288 vs. NCBI nr
Match: TYK21721.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 498.4 bits (1282), Expect = 5.1e-137
Identity = 242/335 (72.24%), Postives = 282/335 (84.18%), Query Frame = 0

Query: 29  SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG NTL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           LYNPTG VNSLLA+SAW +QPFS  CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLAESAWRNQPFSTICHPSTVQDLL 350

BLAST of MS010288 vs. NCBI nr
Match: KAG7035797.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 496.5 bits (1277), Expect = 1.9e-136
Identity = 246/343 (71.72%), Postives = 282/343 (82.22%), Query Frame = 0

Query: 20  VGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFL 79
           +G++   Q S++TAMYLLGDSSVDCGLN L+YPLLHRNFS+ PC    D+D +TLLPH L
Sbjct: 25  LGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLL 84

Query: 80  AKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLR 139
           A+++GVP+SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LR
Sbjct: 85  AEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLR 144

Query: 140 LHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRN 199
           L LG DTA  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRN
Sbjct: 145 LQLGLDTARHFIQSSLFYFSFGEVDFI---LDSSRGK----NNLKFPQLLASQMSVAIRN 204

Query: 200 LEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV 259
           L EAGA+KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV
Sbjct: 205 LHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIV 264

Query: 260 ----KFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSR 319
               + G  R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC R
Sbjct: 265 MLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGR 324

Query: 320 VGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           V R VWWDLYNPTGAVNSLLA+SAW DQPFS FC PST+ DLV
Sbjct: 325 VRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 356

BLAST of MS010288 vs. NCBI nr
Match: XP_022958402.1 (GDSL esterase/lipase At1g71250-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 496.5 bits (1277), Expect = 1.9e-136
Identity = 246/343 (71.72%), Postives = 282/343 (82.22%), Query Frame = 0

Query: 20  VGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFL 79
           +G++   Q S++TAMYLLGDSSVDCGLN L+YPLLHRNFS+ PC    D+D +TLLPH L
Sbjct: 45  LGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLL 104

Query: 80  AKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLR 139
           A+++GVP+SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LR
Sbjct: 105 AEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLR 164

Query: 140 LHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRN 199
           L LG DTA  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRN
Sbjct: 165 LQLGLDTARHFIQSSLFYFSFGEVDFI---LDSSRGK----NNLKFPQLLASQMSVAIRN 224

Query: 200 LEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV 259
           L EAGA+KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV
Sbjct: 225 LHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIV 284

Query: 260 ----KFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSR 319
               + G  R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC R
Sbjct: 285 MLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGR 344

Query: 320 VGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           V R VWWDLYNPTGAVNSLLA+SAW DQPFS FC PST+ DLV
Sbjct: 345 VRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 376

BLAST of MS010288 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 2.4e-33
Identity = 104/364 (28.57%), Postives = 172/364 (47.25%), Query Frame = 0

Query: 11  VLLGMVAVAVGVADPPQV---SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADA 70
           VL+  + V+V +  P  V   + V AM++LGDS VD G N     +   NF  +  + + 
Sbjct: 16  VLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNY 75

Query: 71  D-----ADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMS-SSRSY 130
                 ++  T +   LA+ + +P   P      S   IL G+N+ S  A I+  S  +Y
Sbjct: 76  QPTGRFSNGLTFI-DLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNY 135

Query: 131 Q---SLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLL-NSTGKQ 190
               SLNQQ+      +  LR  +       ++  S+  L FG  D+IN YL+ N     
Sbjct: 136 GGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSS 195

Query: 191 LKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEK 250
           ++++  +F  LL+SQ    +  L   G RKI   G+ PLGC P   +   + P       
Sbjct: 196 IRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDR----- 255

Query: 251 GCVKEMNELVAEYNRGMEK---QIVKFGKGRV-VFCDAYKGMMEIVGSPRRYGFEESKSA 310
            CV  +N+++  +N+G++    Q+ +   G + V+ + Y  + +I+ +P  YGF     A
Sbjct: 256 -CVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRA 315

Query: 311 CCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPST 358
           CCG+G     I C+ ++  C    + V+WD ++PT   NS+LA  A+   P     +P  
Sbjct: 316 CCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPSD--AYPVN 370

BLAST of MS010288 vs. ExPASy Swiss-Prot
Match: Q8L5Z1 (GDSL esterase/lipase At1g33811 OS=Arabidopsis thaliana OX=3702 GN=At1g33811 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 3.9e-31
Identity = 97/367 (26.43%), Postives = 167/367 (45.50%), Query Frame = 0

Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSL----FP 66
           +LI + L +      V+ P Q + V  +++ GDS VD G N     L   N+      FP
Sbjct: 8   LLISLNLVLFGFKTTVSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFP 67

Query: 67  CEADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSS---- 126
                           LA+ +G     P Y++    +AIL G NF S  A I   +    
Sbjct: 68  QGTTGRFTNGRTYVDALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNL 127

Query: 127 RSYQSLNQQLRQALDAIQMLRLHLGQDT--AHRFIQSSVFYLSFGEIDFINLYLL-NSTG 186
            ++ S+NQQ+     A+Q +  +   DT    R++   +FY   G  D++N Y + +   
Sbjct: 128 GAHTSMNQQVELYTTAVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYS 187

Query: 187 KQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYD 246
               Y  K F   L+      +  L + GARK+I  G+  +GC P  L+ + N   +   
Sbjct: 188 TSTNYNDKTFAESLIKNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST-- 247

Query: 247 EKGCVKEMNELVAEYNRGMEKQIVKFGKGRV-----VFCDAYKGMMEIVGSPRRYGFEES 306
              C +++N  +  +N  ++K + +  KG++     V+ D+YK   ++  +   YGFE  
Sbjct: 248 -GRCNEKINNAIVVFNTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVV 307

Query: 307 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCH 358
              CCG+G  N  I C+ ++  C    + ++WD ++PT   N LLA S +  + ++   +
Sbjct: 308 DKGCCGVGRNNGQITCLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSRAYT---Y 367

BLAST of MS010288 vs. ExPASy Swiss-Prot
Match: Q9FHQ1 (GDSL esterase/lipase At5g37690 OS=Arabidopsis thaliana OX=3702 GN=At5g37690 PE=2 SV=1)

HSP 1 Score: 128.3 bits (321), Expect = 1.8e-28
Identity = 87/353 (24.65%), Postives = 166/353 (47.03%), Query Frame = 0

Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLNT-LYYPLLHRNFSLFPCE- 66
           +++ + L +V      A P   S++   Y+ GDS  + G N  L Y L   +F  +  + 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 67  ----ADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS 126
               A         +   ++ K+G+    P  + + + +A L+G+N+ S  A I++ +  
Sbjct: 62  SGGKATGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGI 121

Query: 127 Y----QSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGK 186
           Y     + N Q+       +++R  +G   A++ +  +++++  G  D++N +L      
Sbjct: 122 YFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMAD 181

Query: 187 QLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTP--RVLSLWRNSPGAVY 246
             +Y   EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R       
Sbjct: 182 GQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRM------ 241

Query: 247 DEKGCVKEMNELVAEYNRGMEKQIVKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEES 306
               C+  +NE V E+N   +K ++   K     +  F D Y  +++++ +P  YGF+ +
Sbjct: 242 ----CLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIA 301

Query: 307 KSACCGLGWYNASIG--CVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWS 342
            ++CC +   + S+G  C+     C      V+WD ++P+ + N +LAD  +S
Sbjct: 302 NTSCCNV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFS 340

BLAST of MS010288 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.3e-26
Identity = 90/341 (26.39%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 33  AMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTL-----LPHFLAKKIGVPY 92
           A ++ GDS VD G N  Y P L R  + FP   D             +  + A  +G+P 
Sbjct: 30  AFFVFGDSLVDSG-NNNYIPTLAR-ANYFPYGIDFGFPTGRFCNGRTVVDYGATYLGLPL 89

Query: 93  SQPLYTQNGSIEAILNGLNFGSPQATIM-SSSRSY---QSLNQQLRQALDAIQMLRLHLG 152
             P  +     +  L G+N+ S  A I+  + R Y    + N Q+ Q    I++      
Sbjct: 90  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 153 QDTA--HRFIQSSVFYLSFGEIDFINLYLL-NSTGKQLKYKGKEFGRLLVSQMVIAIRNL 212
           Q+ A   +++  S+  ++ G  D+IN YL+         Y G+++  LL+  +   I  L
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 209

Query: 213 EEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI-- 272
              GARK++  G  PLGC P  LS+   +     +  GCV ++N +V+ +N  ++     
Sbjct: 210 YNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCVTKINNMVSMFNSRLKDLANT 269

Query: 273 --VKFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRV 332
                     V+ + +    ++V +P RYG   S  ACCG G Y  ++ C+ ++  C   
Sbjct: 270 LNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDR 329

Query: 333 GRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDL 358
            + V+WD ++PT   N ++A + +S    + + +P +V +L
Sbjct: 330 NQYVFWDAFHPTETANKIIAHNTFSKS--ANYSYPISVYEL 361

BLAST of MS010288 vs. ExPASy Swiss-Prot
Match: Q9SIZ6 (GDSL esterase/lipase At2g40250 OS=Arabidopsis thaliana OX=3702 GN=At2g40250 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.9e-25
Identity = 90/349 (25.79%), Postives = 166/349 (47.56%), Query Frame = 0

Query: 8   LIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNF----SLFPC 67
           LI +LL  +      +  P    +TA+Y  GDS+VD G N     L   N       FP 
Sbjct: 14  LINILLLQLLNLTNASPSPP---ITALYAFGDSTVDSGNNNYIPTLFQSNHPPYGKSFPS 73

Query: 68  EADADA-DASTLLPHFLAKKIGVPYSQPLYTQNGSIEAI--LNGLNFGSPQATI---MSS 127
           +          L   F+   +G+  + P Y  N S++ +  L G++F S    +    + 
Sbjct: 74  KLSTGRFSDGKLATDFIVSSLGLKPTLPAYL-NPSVKPVDLLTGVSFASAGGGLDDRTAK 133

Query: 128 SRSYQSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFI-NLY--LLNST 187
           S    ++++Q     +A+  ++  +G    +R I+++VF +S G  D I N+Y  +L S 
Sbjct: 134 SSLTITMDKQWSYFEEALGKMKSLVGDSETNRVIKNAVFVISAGTNDMIFNVYDHVLGSL 193

Query: 188 GKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVY 247
                Y+       L++++ + ++ L EAGAR+I   G+ P+GC P  ++L   +   ++
Sbjct: 194 ISVSDYQDS-----LLTKVEVFVQRLYEAGARRITIAGLPPIGCLPVQVTLTSINTPRIF 253

Query: 248 DEKGCVKEMNELVAEYNRGMEKQIV----KFGKGRVVFCDAYKGMMEIVGSPRRYGFEES 307
             + C +  N+    YN+ ++K I     +F   +V++ D Y  +++++  PR+YG EE+
Sbjct: 254 HHRICTEHQNDDSRVYNQKLQKLIFGLSQRFRGSKVLYLDIYSPLIDMIKHPRKYGLEET 313

Query: 308 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSA 340
              CCG G   A   C  +   C  V + +++D  +P+    S++A  A
Sbjct: 314 LRGCCGTGLLEAGPLCQPLSRTCDDVSKYLFFDSVHPSQTAYSVIASFA 353

BLAST of MS010288 vs. ExPASy TrEMBL
Match: A0A0A0L1P2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G269190 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.7e-138
Identity = 244/338 (72.19%), Postives = 285/338 (84.32%), Query Frame = 0

Query: 27  QVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVP 86
           Q SNV AM+LLGDSSVDCG NTL+YPLLHRNFSL PC+A+A    S+LLP  LA KIG+P
Sbjct: 25  QESNVNAMFLLGDSSVDCGFNTLFYPLLHRNFSLLPCDANA---TSSLLPFLLADKIGLP 84

Query: 87  YSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDT 146
           ++QP Y QN S+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  
Sbjct: 85  HAQPFYNQNESLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHA 144

Query: 147 AHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGAR 206
           A  FIQSS+FYLSFGE+DFINLYLL S+  + KY G+EF RLLVSQMVIAIRNL+EAGAR
Sbjct: 145 ARHFIQSSLFYLSFGEVDFINLYLLKSS--ERKYGGEEFARLLVSQMVIAIRNLQEAGAR 204

Query: 207 KIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FG 266
           KI+CMGILPLGC+PRVLS WR+SP    D+KGCVKEMNELV +YN  ME+++VK    FG
Sbjct: 205 KIVCMGILPLGCSPRVLSEWRDSPADTLDKKGCVKEMNELVGKYNEVMEEEMVKLNAEFG 264

Query: 267 KGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVW 326
             +++FCD YKGMMEI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VW
Sbjct: 265 DTQMIFCDVYKGMMEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVW 324

Query: 327 WDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLVW 360
           WDLYNPTG VN+LLADSAW ++PFS  C PST+QDL++
Sbjct: 325 WDLYNPTGVVNALLADSAWGNRPFSTICRPSTIQDLLF 357

BLAST of MS010288 vs. ExPASy TrEMBL
Match: A0A5A7TDD0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G00690 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.4e-138
Identity = 243/335 (72.54%), Postives = 282/335 (84.18%), Query Frame = 0

Query: 29  SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG NTL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           LYNPTG VNSLLADSAW +QPFS  CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

BLAST of MS010288 vs. ExPASy TrEMBL
Match: A0A1S3BNP3 (GDSL esterase/lipase At1g71250-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491620 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.4e-138
Identity = 243/335 (72.54%), Postives = 282/335 (84.18%), Query Frame = 0

Query: 29  SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG NTL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNGQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           LYNPTG VNSLLADSAW +QPFS  CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLADSAWRNQPFSTICHPSTVQDLL 350

BLAST of MS010288 vs. ExPASy TrEMBL
Match: A0A5D3DDH5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00910 PE=3 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.5e-137
Identity = 242/335 (72.24%), Postives = 282/335 (84.18%), Query Frame = 0

Query: 29  SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFLAKKIGVPYS 88
           SNVTAM+LLGDSSVDCG NTL+YP LHRNFSL PC+ +A    S+LLP  LA KIG+P++
Sbjct: 21  SNVTAMFLLGDSSVDCGFNTLFYPRLHRNFSLLPCDTNA---TSSLLPFLLADKIGLPHA 80

Query: 89  QPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLRLHLGQDTAH 148
           QP Y QNGS+EAILNGLNFGSPQATIMS+ +SYQSLNQQLRQ LDAIQ+LRL LGQ  A 
Sbjct: 81  QPWYNQNGSLEAILNGLNFGSPQATIMSNEQSYQSLNQQLRQVLDAIQLLRLQLGQHAAR 140

Query: 149 RFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKI 208
            FIQSS+FYLSFGE+DFINLYLL S+ +  KY G+EF RLLVSQMVIAIRNL+EAGARKI
Sbjct: 141 HFIQSSLFYLSFGEVDFINLYLLKSSAR--KYGGEEFARLLVSQMVIAIRNLQEAGARKI 200

Query: 209 ICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIVK----FGKG 268
           +CMGILPLGC+PRVLS WR+SP   +D+KGCVKEMNEL+ +YN  M +++VK    F   
Sbjct: 201 VCMGILPLGCSPRVLSEWRDSPVDTFDKKGCVKEMNELIGKYNEVMGEEMVKLNAEFEDT 260

Query: 269 RVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNAS-IGCVAMEMACSRVGRSVWWD 328
           +++FCD YKGM EI+G+P RYGF+ESKSACCG+GWYNAS +GCVAME+AC  V R VWWD
Sbjct: 261 QMIFCDVYKGMTEIIGNPTRYGFKESKSACCGVGWYNASAVGCVAMEIACRDVRRYVWWD 320

Query: 329 LYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           LYNPTG VNSLLA+SAW +QPFS  CHPSTVQDL+
Sbjct: 321 LYNPTGVVNSLLAESAWRNQPFSTICHPSTVQDLL 350

BLAST of MS010288 vs. ExPASy TrEMBL
Match: A0A6J1H1Q6 (GDSL esterase/lipase At1g71250-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459637 PE=3 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 9.3e-137
Identity = 246/343 (71.72%), Postives = 282/343 (82.22%), Query Frame = 0

Query: 20  VGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTLLPHFL 79
           +G++   Q S++TAMYLLGDSSVDCGLN L+YPLLHRNFS+ PC    D+D +TLLPH L
Sbjct: 45  LGLSLAAQGSSITAMYLLGDSSVDCGLNALFYPLLHRNFSIIPC----DSDGTTLLPHLL 104

Query: 80  AKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRSYQSLNQQLRQALDAIQMLR 139
           A+++GVP+SQP Y QNGSIEAILNGL+FGSPQATI+S  +S+QSLNQQLRQ LD +Q+LR
Sbjct: 105 AEEMGVPHSQPWYNQNGSIEAILNGLSFGSPQATIVSGGQSHQSLNQQLRQVLDTLQLLR 164

Query: 140 LHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIRN 199
           L LG DTA  FIQSS+FY SFGE+DFI   L +S GK       +F +LL SQM +AIRN
Sbjct: 165 LQLGLDTARHFIQSSLFYFSFGEVDFI---LDSSRGK----NNLKFPQLLASQMSVAIRN 224

Query: 200 LEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQIV 259
           L EAGA+KI+CMGILPLGCTPRVLS WR+SP AVYDEKGCVKEMNELV  YN  MEKQIV
Sbjct: 225 LHEAGAKKIVCMGILPLGCTPRVLSQWRDSPAAVYDEKGCVKEMNELVVNYNELMEKQIV 284

Query: 260 ----KFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSR 319
               + G  R+VFCDAYKGMMEI+G+PRRYGFEES+SACCGLG YNASIGCVA E+AC R
Sbjct: 285 MLNEQLGDARMVFCDAYKGMMEIIGNPRRYGFEESRSACCGLGSYNASIGCVAKEIACGR 344

Query: 320 VGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
           V R VWWDLYNPTGAVNSLLA+SAW DQPFS FC PST+ DLV
Sbjct: 345 VRRHVWWDLYNPTGAVNSLLANSAWRDQPFSSFCRPSTIGDLV 376

BLAST of MS010288 vs. TAIR 10
Match: AT4G10950.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-48
Identity = 105/344 (30.52%), Postives = 176/344 (51.16%), Query Frame = 0

Query: 26  PQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADAST---LLPHFLAKK 85
           P +  V A+++ GDSSVD G N     L   +    P   D D    T            
Sbjct: 64  PSLPFVPALFVFGDSSVDSGTNNFLGTLARAD--RLPYGRDFDTHQPTGRFCNGRIPVDY 123

Query: 86  IGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS----YQSLNQQLRQALDAIQML 145
           +G+P+      Q G++E +  G+N+ S  A I+ SS S      S   Q+ Q +D  Q +
Sbjct: 124 LGLPFVPSYLGQTGTVEDMFQGVNYASAGAGIILSSGSELGQRVSFAMQVEQFVDTFQQM 183

Query: 146 RLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGKQLKYKGKEFGRLLVSQMVIAIR 205
            L +G+  + R + +SVFY+S G  D+I+ Y+ N +  Q  Y    F + L S M   ++
Sbjct: 184 ILSIGEKASERLVSNSVFYISIGVNDYIHFYIRNISNVQNLYTPWNFNQFLASNMRQELK 243

Query: 206 NLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI 265
            L     R+++ MG+ P+GC P  +  +R+  G       C +E+N ++ E N  M   +
Sbjct: 244 TLYNVKVRRMVVMGLPPIGCAPYYMWKYRSQNGE------CAEEVNSMIMESNFVMRYTV 303

Query: 266 VKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACS 325
            K  +      +++CD ++  M+I+ + + YGF E+  ACCGLG Y   + C++ EMACS
Sbjct: 304 DKLNRELPGASIIYCDVFQSAMDILRNHQHYGFNETTDACCGLGRYKGWLPCISPEMACS 363

Query: 326 RVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDLV 359
                +WWD ++PT AVN++LAD+ W+ +     C+P+ ++ ++
Sbjct: 364 DASGHLWWDQFHPTDAVNAILADNVWNGRHVD-MCYPTNLETML 398

BLAST of MS010288 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 144.4 bits (363), Expect = 1.7e-34
Identity = 104/364 (28.57%), Postives = 172/364 (47.25%), Query Frame = 0

Query: 11  VLLGMVAVAVGVADPPQV---SNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADA 70
           VL+  + V+V +  P  V   + V AM++LGDS VD G N     +   NF  +  + + 
Sbjct: 16  VLILALTVSVILQQPELVTGQARVPAMFVLGDSLVDAGNNNFLQTVARANFLPYGIDMNY 75

Query: 71  D-----ADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMS-SSRSY 130
                 ++  T +   LA+ + +P   P      S   IL G+N+ S  A I+  S  +Y
Sbjct: 76  QPTGRFSNGLTFI-DLLARLLEIPSPPPFADPTTSGNRILQGVNYASAAAGILDVSGYNY 135

Query: 131 Q---SLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLL-NSTGKQ 190
               SLNQQ+      +  LR  +       ++  S+  L FG  D+IN YL+ N     
Sbjct: 136 GGRFSLNQQMVNLETTLSQLRTMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSS 195

Query: 191 LKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEK 250
           ++++  +F  LL+SQ    +  L   G RKI   G+ PLGC P   +   + P       
Sbjct: 196 IRFRPPDFANLLLSQYARQLLTLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDR----- 255

Query: 251 GCVKEMNELVAEYNRGMEK---QIVKFGKGRV-VFCDAYKGMMEIVGSPRRYGFEESKSA 310
            CV  +N+++  +N+G++    Q+ +   G + V+ + Y  + +I+ +P  YGF     A
Sbjct: 256 -CVDSVNQILGTFNQGLKSLVDQLNQRSPGAIYVYGNTYSAIGDILNNPAAYGFSVVDRA 315

Query: 311 CCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPST 358
           CCG+G     I C+ ++  C    + V+WD ++PT   NS+LA  A+   P     +P  
Sbjct: 316 CCGIGRNQGQITCLPLQTPCPNRNQYVFWDAFHPTQTANSILARRAFYGPPSD--AYPVN 370

BLAST of MS010288 vs. TAIR 10
Match: AT1G33811.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 137.1 bits (344), Expect = 2.8e-32
Identity = 97/367 (26.43%), Postives = 167/367 (45.50%), Query Frame = 0

Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLNTLYYPLLHRNFSL----FP 66
           +LI + L +      V+ P Q + V  +++ GDS VD G N     L   N+      FP
Sbjct: 8   LLISLNLVLFGFKTTVSQPQQQAQVPCLFIFGDSLVDNGNNNRLLSLARANYRPYGIDFP 67

Query: 67  CEADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSS---- 126
                           LA+ +G     P Y++    +AIL G NF S  A I   +    
Sbjct: 68  QGTTGRFTNGRTYVDALAQILGFRNYIPPYSRIRG-QAILRGANFASGAAGIRDETGDNL 127

Query: 127 RSYQSLNQQLRQALDAIQMLRLHLGQDT--AHRFIQSSVFYLSFGEIDFINLYLL-NSTG 186
            ++ S+NQQ+     A+Q +  +   DT    R++   +FY   G  D++N Y + +   
Sbjct: 128 GAHTSMNQQVELYTTAVQQMLRYFRGDTNELQRYLSRCIFYSGMGSNDYLNNYFMPDFYS 187

Query: 187 KQLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYD 246
               Y  K F   L+      +  L + GARK+I  G+  +GC P  L+ + N   +   
Sbjct: 188 TSTNYNDKTFAESLIKNYTQQLTRLYQFGARKVIVTGVGQIGCIPYQLARYNNRNNST-- 247

Query: 247 EKGCVKEMNELVAEYNRGMEKQIVKFGKGRV-----VFCDAYKGMMEIVGSPRRYGFEES 306
              C +++N  +  +N  ++K + +  KG++     V+ D+YK   ++  +   YGFE  
Sbjct: 248 -GRCNEKINNAIVVFNTQVKKLVDRLNKGQLKGAKFVYLDSYKSTYDLAVNGAAYGFEVV 307

Query: 307 KSACCGLGWYNASIGCVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCH 358
              CCG+G  N  I C+ ++  C    + ++WD ++PT   N LLA S +  + ++   +
Sbjct: 308 DKGCCGVGRNNGQITCLPLQTPCPDRTKYLFWDAFHPTETANILLAKSNFYSRAYT---Y 367

BLAST of MS010288 vs. TAIR 10
Match: AT5G37690.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-29
Identity = 87/353 (24.65%), Postives = 166/353 (47.03%), Query Frame = 0

Query: 7   VLIGVLLGMVAVAVGVADPPQVSNVTAMYLLGDSSVDCGLNT-LYYPLLHRNFSLFPCE- 66
           +++ + L +V      A P   S++   Y+ GDS  + G N  L Y L   +F  +  + 
Sbjct: 2   MILRLALAIVISTYATAQPASTSSLVT-YIFGDSLTEVGNNNFLQYSLARADFPYYGVDF 61

Query: 67  ----ADADADASTLLPHFLAKKIGVPYSQPLYTQNGSIEAILNGLNFGSPQATIMSSSRS 126
               A         +   ++ K+G+    P  + + + +A L+G+N+ S  A I++ +  
Sbjct: 62  SGGKATGRFTNGRTIGDIISTKLGILSPPPYLSLSQNDDAFLSGINYASGGAGILNETGI 121

Query: 127 Y----QSLNQQLRQALDAIQMLRLHLGQDTAHRFIQSSVFYLSFGEIDFINLYLLNSTGK 186
           Y     + N Q+       +++R  +G   A++ +  +++++  G  D++N +L      
Sbjct: 122 YFIQRLTFNDQINCFKKTKEVIRAKIGDGAANKHVNDAMYFIGLGSNDYVNNFLQPFMAD 181

Query: 187 QLKYKGKEFGRLLVSQMVIAIRNLEEAGARKIICMGILPLGCTP--RVLSLWRNSPGAVY 246
             +Y   EF  LL S +   +  + + GARK+I  G+ PLGC P  RV S  R       
Sbjct: 182 GQQYTHDEFVELLTSTLHNQLTTIYKLGARKVIFHGLGPLGCIPSQRVKSKTRM------ 241

Query: 247 DEKGCVKEMNELVAEYNRGMEKQIVKFGK----GRVVFCDAYKGMMEIVGSPRRYGFEES 306
               C+  +NE V E+N   +K ++   K     +  F D Y  +++++ +P  YGF+ +
Sbjct: 242 ----CLNRVNEWVLEFNSRTKKLLIDLNKRLPGAKFSFADTYPAVLDLINNPTHYGFKIA 301

Query: 307 KSACCGLGWYNASIG--CVAMEMACSRVGRSVWWDLYNPTGAVNSLLADSAWS 342
            ++CC +   + S+G  C+     C      V+WD ++P+ + N +LAD  +S
Sbjct: 302 NTSCCNV---DTSVGGLCLPNSKMCKNRQDFVFWDAFHPSDSANQILADHLFS 340

BLAST of MS010288 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 122.1 bits (305), Expect = 9.2e-28
Identity = 90/341 (26.39%), Postives = 157/341 (46.04%), Query Frame = 0

Query: 33  AMYLLGDSSVDCGLNTLYYPLLHRNFSLFPCEADADADASTL-----LPHFLAKKIGVPY 92
           A ++ GDS VD G N  Y P L R  + FP   D             +  + A  +G+P 
Sbjct: 30  AFFVFGDSLVDSG-NNNYIPTLAR-ANYFPYGIDFGFPTGRFCNGRTVVDYGATYLGLPL 89

Query: 93  SQPLYTQNGSIEAILNGLNFGSPQATIM-SSSRSY---QSLNQQLRQALDAIQMLRLHLG 152
             P  +     +  L G+N+ S  A I+  + R Y    + N Q+ Q    I++      
Sbjct: 90  VPPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFF 149

Query: 153 QDTA--HRFIQSSVFYLSFGEIDFINLYLL-NSTGKQLKYKGKEFGRLLVSQMVIAIRNL 212
           Q+ A   +++  S+  ++ G  D+IN YL+         Y G+++  LL+  +   I  L
Sbjct: 150 QNPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRL 209

Query: 213 EEAGARKIICMGILPLGCTPRVLSLWRNSPGAVYDEKGCVKEMNELVAEYNRGMEKQI-- 272
              GARK++  G  PLGC P  LS+   +     +  GCV ++N +V+ +N  ++     
Sbjct: 210 YNLGARKMVLAGSGPLGCIPSQLSMVTGN-----NTSGCVTKINNMVSMFNSRLKDLANT 269

Query: 273 --VKFGKGRVVFCDAYKGMMEIVGSPRRYGFEESKSACCGLGWYNASIGCVAMEMACSRV 332
                     V+ + +    ++V +P RYG   S  ACCG G Y  ++ C+ ++  C   
Sbjct: 270 LNTTLPGSFFVYQNVFDLFHDMVVNPSRYGLVVSNEACCGNGRYGGALTCLPLQQPCLDR 329

Query: 333 GRSVWWDLYNPTGAVNSLLADSAWSDQPFSRFCHPSTVQDL 358
            + V+WD ++PT   N ++A + +S    + + +P +V +L
Sbjct: 330 NQYVFWDAFHPTETANKIIAHNTFSKS--ANYSYPISVYEL 361