MS010286 (gene) Bitter gourd (TR) v1

Overview
NameMS010286
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionF-box/WD-40 repeat-containing protein
Locationscaffold391: 1404090 .. 1405754 (+)
RNA-Seq ExpressionMS010286
SyntenyMS010286
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGAATGCCAGAAGTCTGCCGGGTTTTATAAGGATTCAAGAGTTATTGCGAAAAATTATTGTGAAAAATCAACCGCTGGATCAAGTTATTTCAACCTCAAAGGCAAACCGGAGGGAATTCTATCTGGCACATCGGAATTTGGCTTGGAAAAGGGAAATACTACTTCATCTACTCCGAAGTCAGTCTCTAAAATTGTACCCATTGGTTGTCGGTCAATTACCGACCTTCCGCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCAAAGGAGCTTGGCATTGTATCTTGTGCGTCGACGATTCTTCATAGGCTAGCATCTGAACACCATGTGTGGAAAGAATTCTACAGCGAGAGATGGGGACTTCCAGCTGCATCGGCACCTGTGGCCGCTTCTGGATTTCCCGATGAGAAGTCATGGAAAGATCTTTTTGTGGAGAGAGAGTTTAGGAGCAGAACTTTTATGGGACGATACAGTATGGAGGTTTTGCATGGTCATACTGAAGCAGTTCGTACAGTGTTTCTTCTGGCTTCTGCAAAACTTATATTTACTTCTGGATATGACTCAATTGTGAGAATCTGGGGCTTGGAAGAAGGATTATCCATTGTTTCATCGCGATCACTTGGTTGTACCATTCGTGCACTTGCAGCTGATAGAAAGTTATTGGTTGCTGGAGGTACTGATGGCTTTATCCACTGTTGGAAGGCAATAGAGGGGCTTCCTCACTTGTTTGATGTGAAAGGCCCTCAAAATCACAATACTGAGTTCCGGCTTTGGGAGCATGAGGGACCCATAACCTCACTTGCTCTGGACCTAACAAGGATTTATAGCAGTTCGTGGGACATGACTGTTCGAGTATGGGATCGGTTTTCACAAAAGTGCTTAAGGATCTTGAGGCACGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACGGTTGCCAGCACATCAGGTTCCGATGTATACGTCTGGGATACTAGTAGTGGGGAGTTGGCTACTATCATTCATGATGCTCATGTTGGCAAGGCTTATGCCTTGGCACGAAGCCATACAGGGGATTTTCTATTTACTGGAGGGGAAGATGGTGCAATTCACATGTTTGATATTACTAACCGCAGTGTTGATACAACCGCTCAGCTTGTTGGATCATGGATTCCTCATTCGGGTCCAGTTTATTCCCTAGCATTTGAGTTTCCGTGGCTGGTTTCAGCTTCAAGTGATGGGAAACTCTCACTCATTGACGTGAGAGCATTGCTTAGGACGAGGACACGAACTTTGTGGAAGAGAGATTCCGTGGGACAGCATGCCAGGAGTATCAGTGTGGAGCCTCCGCAGAGAATGTTGCACGGATTTGGGAGCAATTTGTTTGGTGTGGATATTGGTGCGGATCGAATCGTTTGTGGAGGAGAGGAAGGTGTTGTGAGGATCTGGAACTTTTCGCAGGCTCTGGAAACTGAAAGGAGAGCTCGTGCTCTAAGAGGAATTAGGTTAGAGAATAGGATGAGGCGGCGTAGGCTTCAAATAGAAATGAACACCAAAGGTGGTGGTGGTAGGAGTGATCAGTGCTCGGTTGCGGCCAAGAAAACACCATTGAACAGCGATCGTGTTAGCGTTTGGCACAGCAAACGGGGAATGAGTGGAAAGCTTAAAGCT

mRNA sequence

ATGGAATTTGAATGCCAGAAGTCTGCCGGGTTTTATAAGGATTCAAGAGTTATTGCGAAAAATTATTGTGAAAAATCAACCGCTGGATCAAGTTATTTCAACCTCAAAGGCAAACCGGAGGGAATTCTATCTGGCACATCGGAATTTGGCTTGGAAAAGGGAAATACTACTTCATCTACTCCGAAGTCAGTCTCTAAAATTGTACCCATTGGTTGTCGGTCAATTACCGACCTTCCGCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCAAAGGAGCTTGGCATTGTATCTTGTGCGTCGACGATTCTTCATAGGCTAGCATCTGAACACCATGTGTGGAAAGAATTCTACAGCGAGAGATGGGGACTTCCAGCTGCATCGGCACCTGTGGCCGCTTCTGGATTTCCCGATGAGAAGTCATGGAAAGATCTTTTTGTGGAGAGAGAGTTTAGGAGCAGAACTTTTATGGGACGATACAGTATGGAGGTTTTGCATGGTCATACTGAAGCAGTTCGTACAGTGTTTCTTCTGGCTTCTGCAAAACTTATATTTACTTCTGGATATGACTCAATTGTGAGAATCTGGGGCTTGGAAGAAGGATTATCCATTGTTTCATCGCGATCACTTGGTTGTACCATTCGTGCACTTGCAGCTGATAGAAAGTTATTGGTTGCTGGAGGTACTGATGGCTTTATCCACTGTTGGAAGGCAATAGAGGGGCTTCCTCACTTGTTTGATGTGAAAGGCCCTCAAAATCACAATACTGAGTTCCGGCTTTGGGAGCATGAGGGACCCATAACCTCACTTGCTCTGGACCTAACAAGGATTTATAGCAGTTCGTGGGACATGACTGTTCGAGTATGGGATCGGTTTTCACAAAAGTGCTTAAGGATCTTGAGGCACGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACGGTTGCCAGCACATCAGGTTCCGATGTATACGTCTGGGATACTAGTAGTGGGGAGTTGGCTACTATCATTCATGATGCTCATGTTGGCAAGGCTTATGCCTTGGCACGAAGCCATACAGGGGATTTTCTATTTACTGGAGGGGAAGATGGTGCAATTCACATGTTTGATATTACTAACCGCAGTGTTGATACAACCGCTCAGCTTGTTGGATCATGGATTCCTCATTCGGGTCCAGTTTATTCCCTAGCATTTGAGTTTCCGTGGCTGGTTTCAGCTTCAAGTGATGGGAAACTCTCACTCATTGACGTGAGAGCATTGCTTAGGACGAGGACACGAACTTTGTGGAAGAGAGATTCCGTGGGACAGCATGCCAGGAGTATCAGTGTGGAGCCTCCGCAGAGAATGTTGCACGGATTTGGGAGCAATTTGTTTGGTGTGGATATTGGTGCGGATCGAATCGTTTGTGGAGGAGAGGAAGGTGTTGTGAGGATCTGGAACTTTTCGCAGGCTCTGGAAACTGAAAGGAGAGCTCGTGCTCTAAGAGGAATTAGGTTAGAGAATAGGATGAGGCGGCGTAGGCTTCAAATAGAAATGAACACCAAAGGTGGTGGTGGTAGGAGTGATCAGTGCTCGGTTGCGGCCAAGAAAACACCATTGAACAGCGATCGTGTTAGCGTTTGGCACAGCAAACGGGGAATGAGTGGAAAGCTTAAAGCT

Coding sequence (CDS)

ATGGAATTTGAATGCCAGAAGTCTGCCGGGTTTTATAAGGATTCAAGAGTTATTGCGAAAAATTATTGTGAAAAATCAACCGCTGGATCAAGTTATTTCAACCTCAAAGGCAAACCGGAGGGAATTCTATCTGGCACATCGGAATTTGGCTTGGAAAAGGGAAATACTACTTCATCTACTCCGAAGTCAGTCTCTAAAATTGTACCCATTGGTTGTCGGTCAATTACCGACCTTCCGCCTGCAATTATATCTGAGATTTTGAACTGCCTTGATCCAAAGGAGCTTGGCATTGTATCTTGTGCGTCGACGATTCTTCATAGGCTAGCATCTGAACACCATGTGTGGAAAGAATTCTACAGCGAGAGATGGGGACTTCCAGCTGCATCGGCACCTGTGGCCGCTTCTGGATTTCCCGATGAGAAGTCATGGAAAGATCTTTTTGTGGAGAGAGAGTTTAGGAGCAGAACTTTTATGGGACGATACAGTATGGAGGTTTTGCATGGTCATACTGAAGCAGTTCGTACAGTGTTTCTTCTGGCTTCTGCAAAACTTATATTTACTTCTGGATATGACTCAATTGTGAGAATCTGGGGCTTGGAAGAAGGATTATCCATTGTTTCATCGCGATCACTTGGTTGTACCATTCGTGCACTTGCAGCTGATAGAAAGTTATTGGTTGCTGGAGGTACTGATGGCTTTATCCACTGTTGGAAGGCAATAGAGGGGCTTCCTCACTTGTTTGATGTGAAAGGCCCTCAAAATCACAATACTGAGTTCCGGCTTTGGGAGCATGAGGGACCCATAACCTCACTTGCTCTGGACCTAACAAGGATTTATAGCAGTTCGTGGGACATGACTGTTCGAGTATGGGATCGGTTTTCACAAAAGTGCTTAAGGATCTTGAGGCACGGTGACTGGGTTTGGGGACTAGTCCCTCATGATACTACGGTTGCCAGCACATCAGGTTCCGATGTATACGTCTGGGATACTAGTAGTGGGGAGTTGGCTACTATCATTCATGATGCTCATGTTGGCAAGGCTTATGCCTTGGCACGAAGCCATACAGGGGATTTTCTATTTACTGGAGGGGAAGATGGTGCAATTCACATGTTTGATATTACTAACCGCAGTGTTGATACAACCGCTCAGCTTGTTGGATCATGGATTCCTCATTCGGGTCCAGTTTATTCCCTAGCATTTGAGTTTCCGTGGCTGGTTTCAGCTTCAAGTGATGGGAAACTCTCACTCATTGACGTGAGAGCATTGCTTAGGACGAGGACACGAACTTTGTGGAAGAGAGATTCCGTGGGACAGCATGCCAGGAGTATCAGTGTGGAGCCTCCGCAGAGAATGTTGCACGGATTTGGGAGCAATTTGTTTGGTGTGGATATTGGTGCGGATCGAATCGTTTGTGGAGGAGAGGAAGGTGTTGTGAGGATCTGGAACTTTTCGCAGGCTCTGGAAACTGAAAGGAGAGCTCGTGCTCTAAGAGGAATTAGGTTAGAGAATAGGATGAGGCGGCGTAGGCTTCAAATAGAAATGAACACCAAAGGTGGTGGTGGTAGGAGTGATCAGTGCTCGGTTGCGGCCAAGAAAACACCATTGAACAGCGATCGTGTTAGCGTTTGGCACAGCAAACGGGGAATGAGTGGAAAGCTTAAAGCT

Protein sequence

MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSSTPKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYSERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRVSVWHSKRGMSGKLKA
Homology
BLAST of MS010286 vs. NCBI nr
Match: XP_022153816.1 (F-box/WD-40 repeat-containing protein At5g21040 [Momordica charantia])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 554/555 (99.82%), Postives = 554/555 (99.82%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST
Sbjct: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60

Query: 61  PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS 120
           PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS
Sbjct: 61  PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS 120

Query: 121 ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA 180
           ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA
Sbjct: 121 ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA 180

Query: 181 SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI 240
           SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI
Sbjct: 181 SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI 240

Query: 241 EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI 300
           EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI
Sbjct: 241 EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI 300

Query: 301 LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF 360
           LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF
Sbjct: 301 LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF 360

Query: 361 TGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR 420
           TGGEDGAIHMFDITNRSVDTT QLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR
Sbjct: 361 TGGEDGAIHMFDITNRSVDTTTQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR 420

Query: 421 ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI 480
           ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI
Sbjct: 421 ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI 480

Query: 481 WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV 540
           WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV
Sbjct: 481 WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV 540

Query: 541 SVWHSKRGMSGKLKA 556
           SVWHSKRGMSGKLKA
Sbjct: 541 SVWHSKRGMSGKLKA 555

BLAST of MS010286 vs. NCBI nr
Match: XP_022958400.1 (F-box/WD-40 repeat-containing protein At5g21040 [Cucurbita moschata])

HSP 1 Score: 1018.1 bits (2631), Expect = 2.9e-293
Identity = 500/561 (89.13%), Postives = 522/561 (93.05%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGFYKDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 1   MAFECQKSAGFYKDSRVIVKNSCEKPTGGSSYFKLKGKSVGIESEKSEFDLEKGDTTSLS 60

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 61  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 120

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRT+F
Sbjct: 121 NERWGLPIPAIPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTIF 180

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 181 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 240

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 300

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELA++IHDAHVGKAYALARSHTGD
Sbjct: 301 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELASVIHDAHVGKAYALARSHTGD 360

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 361 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 421 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 480

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK--GGGGRSDQCSVAAKKTP 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK  GGGGRSD+CSVAAKKTP
Sbjct: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGGRSDRCSVAAKKTP 540

Query: 541 LNSDRVSVWHSKRGMSGKLKA 556
           +NSD VSVWH+KRG+SGKLKA
Sbjct: 541 MNSDNVSVWHNKRGVSGKLKA 561

BLAST of MS010286 vs. NCBI nr
Match: XP_022995408.1 (F-box/WD-40 repeat-containing protein At5g21040 isoform X2 [Cucurbita maxima] >XP_022995409.1 F-box/WD-40 repeat-containing protein At5g21040 isoform X3 [Cucurbita maxima])

HSP 1 Score: 1010.0 bits (2610), Expect = 8.0e-291
Identity = 498/560 (88.93%), Postives = 519/560 (92.68%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGF KDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 4   MAFECQKSAGFCKDSRVIVKNSCEKPTGGSSYFKLKGKSAGIESEKSEFDLEKGDTTSLS 63

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 64  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 123

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRTVF
Sbjct: 124 NERWGLPIPALPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTVF 183

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 184 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 243

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 244 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 303

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELAT+IHDAHVGKAYAL+RSHTGD
Sbjct: 304 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELATVIHDAHVGKAYALSRSHTGD 363

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 364 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 423

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 424 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 483

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK-GGGGRSDQCSVAAKKTPL 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK GGGGRSD+CSVAAKK  +
Sbjct: 484 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGRSDRCSVAAKKKLM 543

Query: 541 NSDRVSVWHSKRGMSGKLKA 556
           NSD VSVWH+KRG+SGKLKA
Sbjct: 544 NSDNVSVWHNKRGVSGKLKA 563

BLAST of MS010286 vs. NCBI nr
Match: XP_023533894.1 (F-box/WD-40 repeat-containing protein At5g21040 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1008.1 bits (2605), Expect = 3.0e-290
Identity = 497/563 (88.28%), Postives = 519/563 (92.18%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGF KDSRVI K+ CEK T GSSYF LKGK  GI S   EF LEKG+TTS +
Sbjct: 1   MAFECQKSAGFCKDSRVIVKDSCEKPTGGSSYFKLKGKAVGIESEKLEFDLEKGDTTSLS 60

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 61  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 120

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRTVF
Sbjct: 121 NERWGLPIPAIPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTVF 180

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 181 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 240

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 300

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELAT+IHDAHVGKAYALARSHTGD
Sbjct: 301 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELATVIHDAHVGKAYALARSHTGD 360

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGE+GAIHMFD+TNR VDT+ QLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 361 FLFTGGENGAIHMFDVTNRHVDTSTQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 421 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 480

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK----GGGGRSDQCSVAAKK 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK    GGGGRSD+CSVAAKK
Sbjct: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGGGGRSDRCSVAAKK 540

Query: 541 TPLNSDRVSVWHSKRGMSGKLKA 556
           TP+NSD VSVWH+KRG+SGKLKA
Sbjct: 541 TPMNSDNVSVWHNKRGVSGKLKA 563

BLAST of MS010286 vs. NCBI nr
Match: XP_022995407.1 (F-box/WD-40 repeat-containing protein At5g21040 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1006.1 bits (2600), Expect = 1.2e-289
Identity = 498/570 (87.37%), Postives = 519/570 (91.05%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGF KDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 4   MAFECQKSAGFCKDSRVIVKNSCEKPTGGSSYFKLKGKSAGIESEKSEFDLEKGDTTSLS 63

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 64  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 123

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRTVF
Sbjct: 124 NERWGLPIPALPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTVF 183

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 184 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 243

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 244 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 303

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELAT+IHDAHVGKAYAL+RSHTGD
Sbjct: 304 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELATVIHDAHVGKAYALSRSHTGD 363

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 364 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 423

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 424 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 483

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGG-----------GRSDQ 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGG           GRSD+
Sbjct: 484 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGPGGGGGGGRGRSDR 543

Query: 541 CSVAAKKTPLNSDRVSVWHSKRGMSGKLKA 556
           CSVAAKK  +NSD VSVWH+KRG+SGKLKA
Sbjct: 544 CSVAAKKKLMNSDNVSVWHNKRGVSGKLKA 573

BLAST of MS010286 vs. ExPASy Swiss-Prot
Match: Q94AD8 (F-box/WD-40 repeat-containing protein At5g21040 OS=Arabidopsis thaliana OX=3702 GN=At5g21040 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.0e-204
Identity = 361/565 (63.89%), Postives = 417/565 (73.81%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           MEFECQ+      D R         S +G    +L+   +G         +E  N     
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDG--------SVEIPNVKLCC 60

Query: 61  PKSVSKIVPIGCR----------SITDLPPAIISEILNCLDPKELGIVSCASTILHRLAS 120
            K    +VP G +          +I DLP A+ISEILNCLDPKELG+VSC ST LHRLAS
Sbjct: 61  QKKKGTLVPSGSKQLLSDKDLSTTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLAS 120

Query: 121 EHHVWKEFYSERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHT 180
           EHH WKEFY ERWGLP      A+SG  DE+SWKDLFVEREFRSRTF+GRYS++ L+GHT
Sbjct: 121 EHHAWKEFYRERWGLPVVFG-AASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHT 180

Query: 181 EAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGT 240
           EAVRTVFLLASAKL+FTSGYDSIVR+W +EEGLSI +S+ LGCTIRALAAD KLLVAGGT
Sbjct: 181 EAVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGT 240

Query: 241 DGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVW 300
           DGFIHCWK+++GL +LFD+ G Q   TEFRLW HEGPITSLALD+T I+S SWDM+VR+W
Sbjct: 241 DGFIHCWKSLDGLRNLFDLTGFQKEKTEFRLWGHEGPITSLALDMTSIFSGSWDMSVRIW 300

Query: 301 DRFSQKCLRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYAL 360
           DR S KC++ LRH DWVWGL PH+TT+ASTSGSDVY+WD SS     II DAH G  Y+L
Sbjct: 301 DRSSMKCVKTLRHSDWVWGLAPHETTLASTSGSDVYIWDVSSETPLAIIPDAHEGTTYSL 360

Query: 361 ARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASS 420
           ARSHTGDFLFTGGEDG I MF+I     +T+  L+  W+PH+ PVYSL+FEFPWLVSAS 
Sbjct: 361 ARSHTGDFLFTGGEDGGIKMFEIRRYGSETSVVLISQWMPHTSPVYSLSFEFPWLVSASG 420

Query: 421 DGKLSLIDVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIV 480
           DGKL+LIDVR LL+T      KR S      S +VEPPQRMLHGFGSNLF VD+G DRIV
Sbjct: 421 DGKLALIDVRKLLKTNRCAYSKRIS------SSTVEPPQRMLHGFGSNLFSVDVGYDRIV 480

Query: 481 CGGEEGVVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAA 540
           CGGEEG VRIWNF+QALE ERR RAL+G+R ENRMRRRR+Q+EMN K   GR DQCS+AA
Sbjct: 481 CGGEEGTVRIWNFTQALEIERRTRALKGMRHENRMRRRRMQMEMNAK--NGRPDQCSIAA 539

Query: 541 KKTPLNSDRVSVWHSKRGMSGKLKA 556
            K P+N +R   WHSKR  SGK KA
Sbjct: 541 HKNPINGERNRAWHSKRRASGKAKA 539

BLAST of MS010286 vs. ExPASy Swiss-Prot
Match: Q00808 (Vegetative incompatibility protein HET-E-1 OS=Podospora anserina OX=2587412 GN=HET-E1 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 9.9e-18
Identity = 92/359 (25.63%), Postives = 151/359 (42.06%), Query Frame = 0

Query: 164  EVLHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALA--AD 223
            + L GH  +V +V   A  + + +   D  ++IW    G    +    G ++ ++A   D
Sbjct: 835  QTLEGHGSSVLSVAFSADGQRVASGSDDKTIKIWDTASGTGTQTLEGHGGSVWSVAFSPD 894

Query: 224  RKLLVAGGTDGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLAL--DLTRIY 283
            R+ + +G  D  I  W A  G                  L  H G + S+A   D  R+ 
Sbjct: 895  RERVASGSDDKTIKIWDAASG-------------TCTQTLEGHGGRVQSVAFSPDGQRVA 954

Query: 284  SSSWDMTVRVWDRFSQKCLRILR-HGDWVWGLV--PHDTTVASTSG-SDVYVWDTSSGEL 343
            S S D T+++WD  S  C + L  HG  V  +   P    VAS SG   + +WDT+SG  
Sbjct: 955  SGSDDHTIKIWDAASGTCTQTLEGHGSSVLSVAFSPDGQRVASGSGDKTIKIWDTASGTC 1014

Query: 344  ATIIHDAHVGKAYALARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPV 403
               + + H G  +++A S  G  + +G +D  I ++D  + +   T +  G W      V
Sbjct: 1015 TQTL-EGHGGSVWSVAFSPDGQRVASGSDDKTIKIWDTASGTCTQTLEGHGGW------V 1074

Query: 404  YSLAF--EFPWLVSASSDGKLSLIDVRALLRTRTRTL-WKRDSV--------GQHARSIS 463
             S+ F  +   + S S D  + + D  A+  T T+TL    DSV        GQ   S S
Sbjct: 1075 QSVVFSPDGQRVASGSDDHTIKIWD--AVSGTCTQTLEGHGDSVWSVAFSPDGQRVASGS 1134

Query: 464  VEPPQRM-----------LHGFGSNLFGVDIGAD--RIVCGGEEGVVRIWNFSQALETE 491
            ++   ++           L G G  +  V    D  R+  G  +G ++IW+ +    T+
Sbjct: 1135 IDGTIKIWDAASGTCTQTLEGHGGWVHSVAFSPDGQRVASGSIDGTIKIWDAASGTCTQ 1171

BLAST of MS010286 vs. ExPASy Swiss-Prot
Match: C5FP68 (Probable E3 ubiquitin ligase complex SCF subunit sconB OS=Arthroderma otae (strain ATCC MYA-4605 / CBS 113480) OX=554155 GN=sconB PE=3 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.9e-17
Identity = 84/305 (27.54%), Postives = 122/305 (40.00%), Query Frame = 0

Query: 75  ITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYSER---------WGL 134
           +T LPP I  +IL+ LD   L   +  S     LA +  VW     +          WGL
Sbjct: 215 VTALPPEISFKILSYLDTASLCNAAQVSRNWRHLADDDVVWHRMCEQHIDRKCEKCGWGL 274

Query: 135 P------------------AASAPVAASGFPDEKSWKDLFVER-EFRSRTFMGRYSMEVL 194
           P                  A    V        + WK ++++R +  +    GR +  + 
Sbjct: 275 PMLDRKRLKDTKRQVQLRAAGKEAVTGRQQQQHRPWKAVYMDRFKVGTNWKYGRCTTTIF 334

Query: 195 HGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLV 254
            GHT  V  + L     ++ T  YD+ ++IW +E G  I + R    TIR L  D   L+
Sbjct: 335 RGHTNGV--MCLQFDDNILATGSYDATIKIWDIETGKEIRTLRGHESTIRCLQFDDTKLI 394

Query: 255 AGGTDGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMT 314
           +G  D  I  W    G                     H+G +  L  D T + S S D T
Sbjct: 395 SGSLDRTIKVWSWRSG-------------ECISTYTGHQGGVLCLHFDSTTLASGSKDNT 454

Query: 315 VRVWDRFSQKCLRILR-HGDWVWGLVPHDT---TVASTSGS-DVYVWDTSSGELATIIHD 347
           +++W+ F  K  +ILR H DWV   V  DT   TV S S    V +WD  +G+     + 
Sbjct: 455 IKIWN-FQDKSTQILRGHTDWV-NAVKLDTASRTVFSASDDLTVRIWDLDTGK-CIHTYA 501

BLAST of MS010286 vs. ExPASy Swiss-Prot
Match: Q61FW2 (F-box/WD repeat-containing protein sel-10 OS=Caenorhabditis briggsae OX=6238 GN=sel-10 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-14
Identity = 68/259 (26.25%), Postives = 109/259 (42.08%), Query Frame = 0

Query: 166 LHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLL 225
           L+GH+  V T  +    + I +   D  V++W  E+G  + + +    T+R +A     L
Sbjct: 294 LNGHSGGVWTSQISQCGRYIVSGSTDRTVKVWRAEDGFLLHTLQGHTSTVRCMAMANTTL 353

Query: 226 VAGGTDGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDM 285
           V G  D  +  W    GL     V+  Q H    R  + +G I         + S  +D 
Sbjct: 354 VTGSRDCTLRVWDIETGL----HVRTLQGHQAAVRCVQFDGNI---------VVSGGYDF 413

Query: 286 TVRVWDRFSQKCLR-ILRHGDWVWGLVPHDTTVASTSGS---DVYVWDTSSGELATIIHD 345
           TV++WD FS KCLR ++ H + V+ L+         SGS    + VWD S  E   +I  
Sbjct: 414 TVKIWDAFSGKCLRTLIGHSNRVYSLLYESERSIVCSGSLDTSIRVWDFSRPEGQELIAF 473

Query: 346 AHVGKAYALARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFE 405
                +        G+ L +   D  + ++DI      T   ++     H   + SL + 
Sbjct: 474 LSGHTSLTSGMQLRGNILVSCNADSHVRVWDIYE---GTCIHILSG---HRSAITSLQWF 533

Query: 406 FPWLVSASS-DGKLSLIDV 420
              LV+ SS DG + L D+
Sbjct: 534 GRGLVATSSDDGSVKLWDI 533

BLAST of MS010286 vs. ExPASy Swiss-Prot
Match: Q0CY32 (Probable E3 ubiquitin ligase complex SCF subunit sconB OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=sconB PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.1e-12
Identity = 91/368 (24.73%), Postives = 144/368 (39.13%), Query Frame = 0

Query: 141 KSWKDLFVER-EFRSRTFMGRYSMEVLHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGL 200
           + WK+++ +R +  +    GR S ++  GHT  V  + L     ++ T  YD+ ++IW  
Sbjct: 314 RPWKEVYKDRFKVGTNWKYGRCSTKIFKGHTNGV--MCLQFEDNILATGSYDATIKIWDT 373

Query: 201 EEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAIEGLPHLFDVKGPQNHNTEF 260
           E G  + +       IR L  D   L++G  D  +  W    G                 
Sbjct: 374 ETGEELRTLYGHESGIRCLQFDDTKLISGSMDRSLKVWNWRTG-------------ECIS 433

Query: 261 RLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRILR-HGDWVWGLVPHDTTVA 320
               H G +  L  D T + S+S D TV++W+ F  K   +LR H DWV  +    T+  
Sbjct: 434 TYTGHRGGVIGLHFDATILASASVDKTVKIWN-FEDKSTCLLRGHTDWVNAVRVDTTSRT 493

Query: 321 STSGSD---VYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLFTGGEDGAIHMFDITN 380
             S SD   V +WD  + +     H  HVG+   +      +F F        H  +  N
Sbjct: 494 VFSASDDCTVRLWDLDTKQCIRTFH-GHVGQVQQVI-PLPREFEFEE------HDAECEN 553

Query: 381 RSVDTTAQLVGSWIPHS----------------GPVYSLAFEFP--WLVSASSDGKLSLI 440
            +V T +   GS  P +                GP +      P  ++V+++ D      
Sbjct: 554 DNVSTVSDDPGSPAPQTTFGVSSIEPASQSSMFGPSFDNGRPAPPRYIVTSALDS----- 613

Query: 441 DVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGV-DIGAD--RIVCGGE 483
                    T  LW+  +              R L  F  +L GV  +GAD  RIV G E
Sbjct: 614 ---------TIRLWETTT-------------GRCLRTFFGHLEGVWALGADTLRIVSGAE 630

BLAST of MS010286 vs. ExPASy TrEMBL
Match: A0A6J1DLU6 (F-box/WD-40 repeat-containing protein At5g21040 OS=Momordica charantia OX=3673 GN=LOC111021242 PE=4 SV=1)

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 554/555 (99.82%), Postives = 554/555 (99.82%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST
Sbjct: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60

Query: 61  PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS 120
           PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS
Sbjct: 61  PKSVSKIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFYS 120

Query: 121 ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA 180
           ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA
Sbjct: 121 ERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLA 180

Query: 181 SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI 240
           SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI
Sbjct: 181 SAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCWKAI 240

Query: 241 EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI 300
           EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI
Sbjct: 241 EGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRI 300

Query: 301 LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF 360
           LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF
Sbjct: 301 LRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGDFLF 360

Query: 361 TGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR 420
           TGGEDGAIHMFDITNRSVDTT QLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR
Sbjct: 361 TGGEDGAIHMFDITNRSVDTTTQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVR 420

Query: 421 ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI 480
           ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI
Sbjct: 421 ALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGVVRI 480

Query: 481 WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV 540
           WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV
Sbjct: 481 WNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLNSDRV 540

Query: 541 SVWHSKRGMSGKLKA 556
           SVWHSKRGMSGKLKA
Sbjct: 541 SVWHSKRGMSGKLKA 555

BLAST of MS010286 vs. ExPASy TrEMBL
Match: A0A6J1H3C5 (F-box/WD-40 repeat-containing protein At5g21040 OS=Cucurbita moschata OX=3662 GN=LOC111459636 PE=4 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.4e-293
Identity = 500/561 (89.13%), Postives = 522/561 (93.05%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGFYKDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 1   MAFECQKSAGFYKDSRVIVKNSCEKPTGGSSYFKLKGKSVGIESEKSEFDLEKGDTTSLS 60

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 61  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 120

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRT+F
Sbjct: 121 NERWGLPIPAIPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTIF 180

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 181 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 240

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 300

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELA++IHDAHVGKAYALARSHTGD
Sbjct: 301 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELASVIHDAHVGKAYALARSHTGD 360

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 361 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 421 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 480

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK--GGGGRSDQCSVAAKKTP 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK  GGGGRSD+CSVAAKKTP
Sbjct: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGGRSDRCSVAAKKTP 540

Query: 541 LNSDRVSVWHSKRGMSGKLKA 556
           +NSD VSVWH+KRG+SGKLKA
Sbjct: 541 MNSDNVSVWHNKRGVSGKLKA 561

BLAST of MS010286 vs. ExPASy TrEMBL
Match: A0A6J1K5M1 (F-box/WD-40 repeat-containing protein At5g21040 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490963 PE=4 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.9e-291
Identity = 498/560 (88.93%), Postives = 519/560 (92.68%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGF KDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 4   MAFECQKSAGFCKDSRVIVKNSCEKPTGGSSYFKLKGKSAGIESEKSEFDLEKGDTTSLS 63

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 64  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 123

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRTVF
Sbjct: 124 NERWGLPIPALPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTVF 183

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 184 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 243

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 244 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 303

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELAT+IHDAHVGKAYAL+RSHTGD
Sbjct: 304 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELATVIHDAHVGKAYALSRSHTGD 363

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 364 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 423

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 424 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 483

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK-GGGGRSDQCSVAAKKTPL 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTK GGGGRSD+CSVAAKK  +
Sbjct: 484 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGRSDRCSVAAKKKLM 543

Query: 541 NSDRVSVWHSKRGMSGKLKA 556
           NSD VSVWH+KRG+SGKLKA
Sbjct: 544 NSDNVSVWHNKRGVSGKLKA 563

BLAST of MS010286 vs. ExPASy TrEMBL
Match: A0A6J1JYT1 (F-box/WD-40 repeat-containing protein At5g21040 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490963 PE=4 SV=1)

HSP 1 Score: 1006.1 bits (2600), Expect = 5.6e-290
Identity = 498/570 (87.37%), Postives = 519/570 (91.05%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAGF KDSRVI KN CEK T GSSYF LKGK  GI S  SEF LEKG+TTS +
Sbjct: 4   MAFECQKSAGFCKDSRVIVKNSCEKPTGGSSYFKLKGKSAGIESEKSEFDLEKGDTTSLS 63

Query: 61  PKSVS-KIVPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  + VP+GCRSITDLPPAIISEILNCLDPKELGIVSC STILHRLASEHHVWKEFY
Sbjct: 64  SKQLGCRNVPLGCRSITDLPPAIISEILNCLDPKELGIVSCVSTILHRLASEHHVWKEFY 123

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           +ERWGL  PA  APVAA+GF DEKSWKD+FVEREFRS+TFMGRYSMEVLHGHTEAVRTVF
Sbjct: 124 NERWGLPIPALPAPVAATGFSDEKSWKDIFVEREFRSKTFMGRYSMEVLHGHTEAVRTVF 183

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           LL SAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCT+RALAAD KLLVAGGTDGFIHCW
Sbjct: 184 LLVSAKLIFTSGYDSIVRIWDLEEGLSIASSRSLGCTVRALAADTKLLVAGGTDGFIHCW 243

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPIT+LALDLTRIYS SWDMTVRVWDRFSQKC
Sbjct: 244 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITTLALDLTRIYSGSWDMTVRVWDRFSQKC 303

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           LRILRHGDWVWGLVPHDTT+ASTSGSDVYVWDT+SGELAT+IHDAHVGKAYAL+RSHTGD
Sbjct: 304 LRILRHGDWVWGLVPHDTTIASTSGSDVYVWDTNSGELATVIHDAHVGKAYALSRSHTGD 363

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           FLFTGGEDGAIHMFD+TNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 364 FLFTGGEDGAIHMFDVTNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 423

Query: 421 DVRALLRTRTRTLWKRDSVGQH-ARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEG 480
           DVRALLRTR RTLWKRDS GQH  RSI VEPPQRMLHGFGSNLFGVDIG DRIVCGGEEG
Sbjct: 424 DVRALLRTRKRTLWKRDSAGQHVGRSIGVEPPQRMLHGFGSNLFGVDIGLDRIVCGGEEG 483

Query: 481 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGG-----------GRSDQ 540
           VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGG           GRSD+
Sbjct: 484 VVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGGPGGGGGGGRGRSDR 543

Query: 541 CSVAAKKTPLNSDRVSVWHSKRGMSGKLKA 556
           CSVAAKK  +NSD VSVWH+KRG+SGKLKA
Sbjct: 544 CSVAAKKKLMNSDNVSVWHNKRGVSGKLKA 573

BLAST of MS010286 vs. ExPASy TrEMBL
Match: A0A5D3DE99 (F-box/WD-40 repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00900 PE=4 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 477/559 (85.33%), Postives = 501/559 (89.62%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           M FECQKSAG Y+DSRVI KNYCEK T GSS FNL+ + EGI S  SE      N  S T
Sbjct: 1   MAFECQKSAGIYRDSRVIGKNYCEKPTTGSSCFNLEAESEGIESDKSE------NIASLT 60

Query: 61  PKSVSKI-VPIGCRSITDLPPAIISEILNCLDPKELGIVSCASTILHRLASEHHVWKEFY 120
            K +  I  P+GCRSITDLPPAIISEILNCLDPKELGIVSC ST+LH +ASEHHVWKEFY
Sbjct: 61  SKHLLSINAPVGCRSITDLPPAIISEILNCLDPKELGIVSCVSTVLHSIASEHHVWKEFY 120

Query: 121 SERWGL--PAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHTEAVRTVF 180
           SERWGL  PA SAPV  +G  DEKSWKDLFVEREFRS+TFMGRY+MEVLHGHTEAVRTVF
Sbjct: 121 SERWGLPVPAQSAPVVPNGLSDEKSWKDLFVEREFRSKTFMGRYTMEVLHGHTEAVRTVF 180

Query: 181 LLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHCW 240
           +LASAKLIFTSGYDSIVRIW LEEGLSI SSRSLGCTIRALAAD KLL+AGGTDGFIHCW
Sbjct: 181 VLASAKLIFTSGYDSIVRIWELEEGLSIASSRSLGCTIRALAADTKLLIAGGTDGFIHCW 240

Query: 241 KAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKC 300
           KAIEGL +LFDVKGP NHN EFRLW HEGPITSLALDLTRIYS SWDMTVRVWDRFS +C
Sbjct: 241 KAIEGLSYLFDVKGPLNHNIEFRLWGHEGPITSLALDLTRIYSGSWDMTVRVWDRFSHQC 300

Query: 301 LRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYALARSHTGD 360
           L ILRHGDWVWGLVPHDTTVASTSGSDVYVWDT+SGELAT+IHDAHVG AYALARSHTGD
Sbjct: 301 LSILRHGDWVWGLVPHDTTVASTSGSDVYVWDTNSGELATVIHDAHVGYAYALARSHTGD 360

Query: 361 FLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420
           F+FTGGEDGAIHMFDITNR VDT+AQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI
Sbjct: 361 FIFTGGEDGAIHMFDITNRHVDTSAQLVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLI 420

Query: 421 DVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIVCGGEEGV 480
           DVR LLRT+ RTLWKRDS GQH +SISVEPPQRMLHGFGSNLFGVDIG DRIVCGGEEGV
Sbjct: 421 DVRPLLRTKKRTLWKRDSRGQHVQSISVEPPQRMLHGFGSNLFGVDIGMDRIVCGGEEGV 480

Query: 481 VRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAAKKTPLN- 540
           VRIWNF+QALE ERRARALRGIRLENRMRRRRLQIEMNTK GG RSDQCS AAKKTP+N 
Sbjct: 481 VRIWNFTQALEAERRARALRGIRLENRMRRRRLQIEMNTKSGGARSDQCSFAAKKTPMNG 540

Query: 541 SDRVSVWHSKRGMSGKLKA 556
            DRVSVWH+KRG+SGKLKA
Sbjct: 541 GDRVSVWHNKRGVSGKLKA 553

BLAST of MS010286 vs. TAIR 10
Match: AT5G21040.1 (F-box protein 2 )

HSP 1 Score: 713.4 bits (1840), Expect = 1.4e-205
Identity = 361/565 (63.89%), Postives = 417/565 (73.81%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           MEFECQ+      D R         S +G    +L+   +G         +E  N     
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDG--------SVEIPNVKLCC 60

Query: 61  PKSVSKIVPIGCR----------SITDLPPAIISEILNCLDPKELGIVSCASTILHRLAS 120
            K    +VP G +          +I DLP A+ISEILNCLDPKELG+VSC ST LHRLAS
Sbjct: 61  QKKKGTLVPSGSKQLLSDKDLSTTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLAS 120

Query: 121 EHHVWKEFYSERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHT 180
           EHH WKEFY ERWGLP      A+SG  DE+SWKDLFVEREFRSRTF+GRYS++ L+GHT
Sbjct: 121 EHHAWKEFYRERWGLPVVFG-AASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHT 180

Query: 181 EAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGT 240
           EAVRTVFLLASAKL+FTSGYDSIVR+W +EEGLSI +S+ LGCTIRALAAD KLLVAGGT
Sbjct: 181 EAVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGT 240

Query: 241 DGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVW 300
           DGFIHCWK+++GL +LFD+ G Q   TEFRLW HEGPITSLALD+T I+S SWDM+VR+W
Sbjct: 241 DGFIHCWKSLDGLRNLFDLTGFQKEKTEFRLWGHEGPITSLALDMTSIFSGSWDMSVRIW 300

Query: 301 DRFSQKCLRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYAL 360
           DR S KC++ LRH DWVWGL PH+TT+ASTSGSDVY+WD SS     II DAH G  Y+L
Sbjct: 301 DRSSMKCVKTLRHSDWVWGLAPHETTLASTSGSDVYIWDVSSETPLAIIPDAHEGTTYSL 360

Query: 361 ARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASS 420
           ARSHTGDFLFTGGEDG I MF+I     +T+  L+  W+PH+ PVYSL+FEFPWLVSAS 
Sbjct: 361 ARSHTGDFLFTGGEDGGIKMFEIRRYGSETSVVLISQWMPHTSPVYSLSFEFPWLVSASG 420

Query: 421 DGKLSLIDVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIV 480
           DGKL+LIDVR LL+T      KR S      S +VEPPQRMLHGFGSNLF VD+G DRIV
Sbjct: 421 DGKLALIDVRKLLKTNRCAYSKRIS------SSTVEPPQRMLHGFGSNLFSVDVGYDRIV 480

Query: 481 CGGEEGVVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAA 540
           CGGEEG VRIWNF+QALE ERR RAL+G+R ENRMRRRR+Q+EMN K   GR DQCS+AA
Sbjct: 481 CGGEEGTVRIWNFTQALEIERRTRALKGMRHENRMRRRRMQMEMNAK--NGRPDQCSIAA 539

Query: 541 KKTPLNSDRVSVWHSKRGMSGKLKA 556
            K P+N +R   WHSKR  SGK KA
Sbjct: 541 HKNPINGERNRAWHSKRRASGKAKA 539

BLAST of MS010286 vs. TAIR 10
Match: AT5G21040.2 (F-box protein 2 )

HSP 1 Score: 713.4 bits (1840), Expect = 1.4e-205
Identity = 361/565 (63.89%), Postives = 417/565 (73.81%), Query Frame = 0

Query: 1   MEFECQKSAGFYKDSRVIAKNYCEKSTAGSSYFNLKGKPEGILSGTSEFGLEKGNTTSST 60
           MEFECQ+      D R         S +G    +L+   +G         +E  N     
Sbjct: 1   MEFECQERLEAANDQR---------SESGLLNTDLRIGNDG--------SVEIPNVKLCC 60

Query: 61  PKSVSKIVPIGCR----------SITDLPPAIISEILNCLDPKELGIVSCASTILHRLAS 120
            K    +VP G +          +I DLP A+ISEILNCLDPKELG+VSC ST LHRLAS
Sbjct: 61  QKKKGTLVPSGSKQLLSDKDLSTTIIDLPQALISEILNCLDPKELGLVSCVSTYLHRLAS 120

Query: 121 EHHVWKEFYSERWGLPAASAPVAASGFPDEKSWKDLFVEREFRSRTFMGRYSMEVLHGHT 180
           EHH WKEFY ERWGLP      A+SG  DE+SWKDLFVEREFRSRTF+GRYS++ L+GHT
Sbjct: 121 EHHAWKEFYRERWGLPVVFG-AASSGLSDERSWKDLFVEREFRSRTFLGRYSIDTLYGHT 180

Query: 181 EAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLGCTIRALAADRKLLVAGGT 240
           EAVRTVFLLASAKL+FTSGYDSIVR+W +EEGLSI +S+ LGCTIRALAAD KLLVAGGT
Sbjct: 181 EAVRTVFLLASAKLVFTSGYDSIVRMWDMEEGLSIAASKPLGCTIRALAADTKLLVAGGT 240

Query: 241 DGFIHCWKAIEGLPHLFDVKGPQNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRVW 300
           DGFIHCWK+++GL +LFD+ G Q   TEFRLW HEGPITSLALD+T I+S SWDM+VR+W
Sbjct: 241 DGFIHCWKSLDGLRNLFDLTGFQKEKTEFRLWGHEGPITSLALDMTSIFSGSWDMSVRIW 300

Query: 301 DRFSQKCLRILRHGDWVWGLVPHDTTVASTSGSDVYVWDTSSGELATIIHDAHVGKAYAL 360
           DR S KC++ LRH DWVWGL PH+TT+ASTSGSDVY+WD SS     II DAH G  Y+L
Sbjct: 301 DRSSMKCVKTLRHSDWVWGLAPHETTLASTSGSDVYIWDVSSETPLAIIPDAHEGTTYSL 360

Query: 361 ARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAFEFPWLVSASS 420
           ARSHTGDFLFTGGEDG I MF+I     +T+  L+  W+PH+ PVYSL+FEFPWLVSAS 
Sbjct: 361 ARSHTGDFLFTGGEDGGIKMFEIRRYGSETSVVLISQWMPHTSPVYSLSFEFPWLVSASG 420

Query: 421 DGKLSLIDVRALLRTRTRTLWKRDSVGQHARSISVEPPQRMLHGFGSNLFGVDIGADRIV 480
           DGKL+LIDVR LL+T      KR S      S +VEPPQRMLHGFGSNLF VD+G DRIV
Sbjct: 421 DGKLALIDVRKLLKTNRCAYSKRIS------SSTVEPPQRMLHGFGSNLFSVDVGYDRIV 480

Query: 481 CGGEEGVVRIWNFSQALETERRARALRGIRLENRMRRRRLQIEMNTKGGGGRSDQCSVAA 540
           CGGEEG VRIWNF+QALE ERR RAL+G+R ENRMRRRR+Q+EMN K   GR DQCS+AA
Sbjct: 481 CGGEEGTVRIWNFTQALEIERRTRALKGMRHENRMRRRRMQMEMNAK--NGRPDQCSIAA 539

Query: 541 KKTPLNSDRVSVWHSKRGMSGKLKA 556
            K P+N +R   WHSKR  SGK KA
Sbjct: 541 HKNPINGERNRAWHSKRRASGKAKA 539

BLAST of MS010286 vs. TAIR 10
Match: AT3G51930.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 68.9 bits (167), Expect = 1.4e-11
Identity = 90/362 (24.86%), Postives = 141/362 (38.95%), Query Frame = 0

Query: 163 MEVLHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVSSRSLG-CTIRALAAD 222
           + VL  H  +V ++ L     L  + G D IV  W  +  L I +    G  +++AL + 
Sbjct: 54  LAVLSAHVGSVSSLALCGEFLLSASQGKDIIV--W-QQPDLKIFAKFGQGDGSVKALVSV 113

Query: 223 RKLLVAGGTDGFIHCWKA-----------IEGLPHLFDVKGP--------QNHNTEFRLW 282
              +     D  I  WK            ++ LP   D  G         Q      RLW
Sbjct: 114 GSKVFTAHQDSRIRVWKVSRRNSENAFRLVDTLPTTKDYLGKFMKQSNYVQTRRNHKRLW 173

Query: 283 -EHEGPITSLALDLTRIYSSSWDMTVRVWDRFSQKCLRILR-HGDWVWGLVPHDTTVAST 342
            EH   I+ LA+    IYS SWD T++VW     KCL  ++ H D + GLV  D  V S 
Sbjct: 174 IEHADSISCLAVHAGIIYSGSWDKTLKVWRLSDLKCLESIKAHDDAINGLVAGDGRVYSA 233

Query: 343 SG-SDVYVW-----------DTSSGEL----ATIIHDAHVGKAYALARSHTGDFLFTGGE 402
           S    V +W            +SS  L    AT+   A V    ++  S  G++++ GG 
Sbjct: 234 SADGKVKIWGKEKRKQIESTSSSSSSLHVLKATLEGRAEV-SVNSVVVSGDGNWVYGGGS 293

Query: 403 DGAIHMFDITNRSVDTTAQLVG-SWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVRALL 462
           DG +  ++   +  D     +G     H+  V  +      + S S+D  +         
Sbjct: 294 DGFVIGWEKKEKEGDFEEWRLGFETRGHNMAVLCMCVVGEMVCSGSADKSIG-------- 353

Query: 463 RTRTRTLWKRDSVGQHARSISV---EPPQRMLHGFGSNLFGVDIGAD-RIVCGGEEGVVR 482
                 LW+R+  G   +   +   E P + L    +N     +GA   +  GG +  +R
Sbjct: 354 ------LWRREVTGMLCKFGVIHGHEGPVKCLQASPNN-----VGAGFMLYSGGLDKSLR 392

BLAST of MS010286 vs. TAIR 10
Match: AT4G34380.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 68.2 bits (165), Expect = 2.4e-11
Identity = 77/302 (25.50%), Postives = 117/302 (38.74%), Query Frame = 0

Query: 178 LLASAKLIFTSGYDSIVRIW-GLEEGLSIVSSRSLGCTIRALAADRKLLVAGGTDGFIHC 237
           L AS  L++T      +R+W  L+E     SS  L   I+A+      +  G  DG I  
Sbjct: 131 LAASGDLLYTGSDSKNIRVWKNLKEHAGFKSSSGL---IKAIVIFGDRIFTGHQDGKIRI 190

Query: 238 WKAIEGLP-----------------------HLFDVKGPQNHNTEFRLWEHEGPITSLAL 297
           WK  +  P                       H  +V+  +N N+     +H   ++SL+L
Sbjct: 191 WKVSKRKPGKHKRVGTLPTFKSMVKSSVNPKHFMEVR--RNRNSV--KTKHNDAVSSLSL 250

Query: 298 D--LTRIYSSSWDMTVRVWDRFSQKCLR-ILRHGDWVWGLVPHDTTVASTSGSD--VYVW 357
           D  L  +YSSSWD T++VW     KCL  I  H D +  ++     +  T  +D  V VW
Sbjct: 251 DVELGLLYSSSWDTTIKVWRIADSKCLESIHAHDDAINSVMSGFDDLVFTGSADGTVKVW 310

Query: 358 DTSSGELATIIHDAHV-----GKAYALARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQ 417
                   T    A V         ALA       ++ G  DG ++ ++ + RS  T   
Sbjct: 311 KRELQGKGTKHTLAQVLLKQENAVTALAVKSQSSIVYCGSSDGLVNYWERSKRSF-TGGI 370

Query: 418 LVGSWIPHSGPVYSLAFEFPWLVSASSDGKLSLIDVRALLRTRTRTLWKRDSVGQHARSI 446
           L G    H   V  L      L+S S+D  +               +W+RD   +  + +
Sbjct: 371 LKG----HKSAVLCLGIAGNLLLSGSADKNI--------------CVWRRDPSDKSHQCL 406

BLAST of MS010286 vs. TAIR 10
Match: AT1G21651.1 (zinc ion binding )

HSP 1 Score: 66.2 bits (160), Expect = 9.2e-11
Identity = 66/299 (22.07%), Postives = 117/299 (39.13%), Query Frame = 0

Query: 148 VEREFRSRTFMGRYSMEVLHGHTEAVRTVFLLASAKLIFTSGYDSIVRIWGLEEGLSIVS 207
           V+ +F  R   G+   + + GH ++V    L      +F+S YD  + IW L++   + +
Sbjct: 502 VDIDFPGRVSEGKVRSKDMRGHQDSV--TGLAVGGGFLFSSSYDRTILIWSLKDFSHVHT 561

Query: 208 SRSLGCTIRAL---AADRKLLVAGGTDGFIHCWKA---IEGLP----------------- 267
            +     + AL        + V+G   G I  W     +E  P                 
Sbjct: 562 FKGHQDKVMALIHIEGTEPVCVSGDGGGGIFVWSTTFPLEEQPLRKWYEPKDWRYTGIHA 621

Query: 268 -------HLFDVKGP--------QNHNTEFRLWEHEGPITSLALDLTRIYSSSWDMTVRV 327
                  H++   G         Q+ +    +  H+  +++L +    +YS SWD TVR+
Sbjct: 622 LAYSEYGHVYTGSGDNTIKAWSLQDGSLLCTMSGHKSVVSTLVVVNGVLYSGSWDGTVRL 681

Query: 328 WDRFSQKCLRILRHGDWVWGLV--------PHDTTVASTSGSDVYVWDTSSGELATIIHD 387
           W       L +L  G+   G+V           T VA+    D+ +W        T++  
Sbjct: 682 WSLSDNSLLTVL--GEETPGIVRSILSLAADDQTLVAAYQNGDIQIWRDD-----TLMKS 741

Query: 388 AHVGKAYALARSHTGDFLFTGGEDGAIHMFDITNRSVDTTAQLVGSWIPHSGPVYSLAF 401
             +     L+ +  G +LFTGG D  I++ +++   +      VGS IP S  + SL +
Sbjct: 742 MKIQNGAILSIAVNGKWLFTGGWDKTINVQELSGDEISVNCAHVGS-IPGSSVITSLLY 790

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153816.10.0e+0099.82F-box/WD-40 repeat-containing protein At5g21040 [Momordica charantia][more]
XP_022958400.12.9e-29389.13F-box/WD-40 repeat-containing protein At5g21040 [Cucurbita moschata][more]
XP_022995408.18.0e-29188.93F-box/WD-40 repeat-containing protein At5g21040 isoform X2 [Cucurbita maxima] >X... [more]
XP_023533894.13.0e-29088.28F-box/WD-40 repeat-containing protein At5g21040 [Cucurbita pepo subsp. pepo][more]
XP_022995407.11.2e-28987.37F-box/WD-40 repeat-containing protein At5g21040 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q94AD82.0e-20463.89F-box/WD-40 repeat-containing protein At5g21040 OS=Arabidopsis thaliana OX=3702 ... [more]
Q008089.9e-1825.63Vegetative incompatibility protein HET-E-1 OS=Podospora anserina OX=2587412 GN=H... [more]
C5FP684.9e-1727.54Probable E3 ubiquitin ligase complex SCF subunit sconB OS=Arthroderma otae (stra... [more]
Q61FW21.8e-1426.25F-box/WD repeat-containing protein sel-10 OS=Caenorhabditis briggsae OX=6238 GN=... [more]
Q0CY322.1e-1224.73Probable E3 ubiquitin ligase complex SCF subunit sconB OS=Aspergillus terreus (s... [more]
Match NameE-valueIdentityDescription
A0A6J1DLU60.0e+0099.82F-box/WD-40 repeat-containing protein At5g21040 OS=Momordica charantia OX=3673 G... [more]
A0A6J1H3C51.4e-29389.13F-box/WD-40 repeat-containing protein At5g21040 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1K5M13.9e-29188.93F-box/WD-40 repeat-containing protein At5g21040 isoform X2 OS=Cucurbita maxima O... [more]
A0A6J1JYT15.6e-29087.37F-box/WD-40 repeat-containing protein At5g21040 isoform X1 OS=Cucurbita maxima O... [more]
A0A5D3DE991.1e-27785.33F-box/WD-40 repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT5G21040.11.4e-20563.89F-box protein 2 [more]
AT5G21040.21.4e-20563.89F-box protein 2 [more]
AT3G51930.11.4e-1124.86Transducin/WD40 repeat-like superfamily protein [more]
AT4G34380.12.4e-1125.50Transducin/WD40 repeat-like superfamily protein [more]
AT1G21651.19.2e-1122.07zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 78..118
e-value: 2.7E-5
score: 33.6
IPR001810F-box domainPFAMPF00646F-boxcoord: 74..119
e-value: 5.6E-5
score: 22.9
IPR001810F-box domainPROSITEPS50181FBOXcoord: 72..118
score: 10.557245
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 159..198
e-value: 0.077
score: 22.1
coord: 332..372
e-value: 0.02
score: 24.1
coord: 381..418
e-value: 0.014
score: 24.5
coord: 204..238
e-value: 55.0
score: 6.0
coord: 294..329
e-value: 0.73
score: 17.9
coord: 445..482
e-value: 3.4
score: 13.6
coord: 254..291
e-value: 0.0011
score: 28.2
IPR001680WD40 repeatPFAMPF00400WD40coord: 263..291
e-value: 0.028
score: 15.3
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 340..381
score: 9.004632
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 166..207
score: 12.279615
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 261..300
score: 9.372231
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 157..379
e-value: 7.1E-35
score: 122.2
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 380..497
e-value: 3.5E-9
score: 37.8
NoneNo IPR availableGENE3D1.20.1280.50coord: 70..156
e-value: 3.8E-9
score: 38.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 515..555
NoneNo IPR availablePANTHERPTHR19855WD40 REPEAT PROTEIN 12, 37coord: 1..552
NoneNo IPR availablePANTHERPTHR19855:SF19F-BOX/WD-40 REPEAT PLANT PROTEINcoord: 1..552
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 166..202
score: 9.704691
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 70..150
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 165..484

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010286.1MS010286.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding