MS010280 (gene) Bitter gourd (TR) v1

Overview


Name	MS010280
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	urease accessory protein F
Location	scaffold391: 1355716 .. 1356399 (-)
RNA-Seq Expression	MS010280
Synteny	MS010280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGCAAGCATTTGCAGTGGAACCAATGGCAGCTGCTTGATTCCGTTCTTCCTACTGGTGGCTTCGCCCACTCATTTGGTCTCGAGGCTGCAATACAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATGTGTTGGACAACACTGGAAGTTTGCTTCTTCCCTTTGTGCACTCAGCAACACTATCTCCTGATTTAGAAACTTGGCAGAAAAATGACAGATTATTGGATGCAATGTTGACTAATGAAGTTGGTCGGAAGGCATCCATTACGCAAGGGTCAGCTCTCATGAGAGTTGCAGCTGTAGTATTTTCCGAAATCCCATCTTTGAAAACGATGAGAGAAACCTTCTCAGGCACTGGGGCTGTTCCATTCCACCATGCTCCCATATTCGGGCTTATTTGCGGGCTACTTGGATGGGATAGCGCAATGTCGCAGAGGGCTTATCTGTTTATTACCATGAGAGATGTTATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACACTGAAAAAATGGATGAACCGTCCTGTTGAAGAAGCTTGTCAATCGGTTCCTCTGTTAGAGACGGTACAGGGCTGCCACATGTACCTGTTTTCTAGACTGTTTTGTTCT

mRNA sequence

ATGGATGGCAAGCATTTGCAGTGGAACCAATGGCAGCTGCTTGATTCCGTTCTTCCTACTGGTGGCTTCGCCCACTCATTTGGTCTCGAGGCTGCAATACAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATGTGTTGGACAACACTGGAAGTTTGCTTCTTCCCTTTGTGCACTCAGCAACACTATCTCCTGATTTAGAAACTTGGCAGAAAAATGACAGATTATTGGATGCAATGTTGACTAATGAAGTTGGTCGGAAGGCATCCATTACGCAAGGGTCAGCTCTCATGAGAGTTGCAGCTGTAGTATTTTCCGAAATCCCATCTTTGAAAACGATGAGAGAAACCTTCTCAGGCACTGGGGCTGTTCCATTCCACCATGCTCCCATATTCGGGCTTATTTGCGGGCTACTTGGATGGGATAGCGCAATGTCGCAGAGGGCTTATCTGTTTATTACCATGAGAGATGTTATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACACTGAAAAAATGGATGAACCGTCCTGTTGAAGAAGCTTGTCAATCGGTTCCTCTGTTAGAGACGGTACAGGGCTGCCACATGTACCTGTTTTCTAGACTGTTTTGTTCT

Coding sequence (CDS)

ATGGATGGCAAGCATTTGCAGTGGAACCAATGGCAGCTGCTTGATTCCGTTCTTCCTACTGGTGGCTTCGCCCACTCATTTGGTCTCGAGGCTGCAATACAAGCTCGCATTGTCTCACAACCAGAAGATCTCAAAACGTTTGTGATCCATGTGTTGGACAACACTGGAAGTTTGCTTCTTCCCTTTGTGCACTCAGCAACACTATCTCCTGATTTAGAAACTTGGCAGAAAAATGACAGATTATTGGATGCAATGTTGACTAATGAAGTTGGTCGGAAGGCATCCATTACGCAAGGGTCAGCTCTCATGAGAGTTGCAGCTGTAGTATTTTCCGAAATCCCATCTTTGAAAACGATGAGAGAAACCTTCTCAGGCACTGGGGCTGTTCCATTCCACCATGCTCCCATATTCGGGCTTATTTGCGGGCTACTTGGATGGGATAGCGCAATGTCGCAGAGGGCTTATCTGTTTATTACCATGAGAGATGTTATTTCTGCTGCAACAAGACTGAATTTAGTAGGACCTTTGGGTGCAGCTGTGTTGCAGCATCAGCTTGCGCTTGTTGCTGAAGACACACTGAAAAAATGGATGAACCGTCCTGTTGAAGAAGCTTGTCAATCGGTTCCTCTGTTAGAGACGGTACAGGGCTGCCACATGTACCTGTTTTCTAGACTGTTTTGTTCT

Protein sequence

MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS

Homology

[Close]

BLAST of MS010280 vs. NCBI nr
Match: XP_022153866.1 (urease accessory protein F [Momordica charantia] >XP_022153867.1 urease accessory protein F [Momordica charantia])

HSP 1 Score: 460.7 bits (1184), Expect = 7.4e-126
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL
Sbjct: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR
Sbjct: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV
Sbjct: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAED LKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS
Sbjct: 181 LQHQLALVAEDILKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 228

[Close]

BLAST of MS010280 vs. NCBI nr
Match: XP_022946416.1 (urease accessory protein F [Cucurbita moschata])

HSP 1 Score: 421.0 bits (1081), Expect = 6.5e-114
Identity = 204/228 (89.47%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+KNDR L+A+LTNEV RKAS+TQGSALMRVAA+VFSEI SLK MR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of MS010280 vs. NCBI nr
Match: XP_023545319.1 (urease accessory protein F [Cucurbita pepo subsp. pepo])

HSP 1 Score: 421.0 bits (1081), Expect = 6.5e-114
Identity = 204/228 (89.47%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 14  MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 73

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+KNDR L+A+LTNEV RKAS+TQGSALMRVAA+VFSEI SLK MR
Sbjct: 74  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 133

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 134 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 193

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 194 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 241

[Close]

BLAST of MS010280 vs. NCBI nr
Match: XP_022999571.1 (urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory protein F [Cucurbita maxima])

HSP 1 Score: 418.7 bits (1075), Expect = 3.2e-113
Identity = 204/228 (89.47%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+KNDR L+A+LTNEV RKASITQGSALMRVAA+VFSEI SLK MR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASITQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 128 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAE TLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 188 LQHQLALVAEGTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of MS010280 vs. NCBI nr
Match: KAG7030122.1 (Urease accessory protein F, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 417.5 bits (1072), Expect = 7.2e-113
Identity = 202/228 (88.60%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LP+GGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 1   MDDKQFHWSQWQLLDSILPSGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 60

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+ NDR L+A+LTNEV RKAS+TQGSALMRVAA+VFSEI SLK MR
Sbjct: 61  PFVHSTTLSPDLETWENNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 120

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 121 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 181 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 228

[Close]

BLAST of MS010280 vs. ExPASy Swiss-Prot
Match: Q9XHZ3 (Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.0e-90
Identity = 156/223 (69.96%), Postives = 190/223 (85.20%), Query Frame = 0


Query: 6   LQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFVHS 65
           +QW+QWQLLDS+LPTGGFAHSFGLEAAIQ R+VS PEDL+T +IHVLDNT SLLLPFV+S
Sbjct: 18  MQWSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYS 77

Query: 66  ATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFSG 125
           A  SPD+ETW K D +L+A LTN+V  KAS++QGSAL R+AA VF+E+P+LK +R+   G
Sbjct: 78  ALKSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLG 137

Query: 126 TGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQL 185
           +  V FHHAPIFGL+CGLLG DS  SQRAYLF+T+RDV+SAATRLN+VGP+GA+V+QH++
Sbjct: 138 SKNVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRI 197

Query: 186 ALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           A+V E  L+KWMNR   EACQ+ PLL+ VQGCH YLFSRLFCS
Sbjct: 198 AIVTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

[Close]

BLAST of MS010280 vs. ExPASy Swiss-Prot
Match: E0ZS46 (Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 SV=1)

HSP 1 Score: 322.0 bits (824), Expect = 5.4e-87
Identity = 153/225 (68.00%), Postives = 192/225 (85.33%), Query Frame = 0


Query: 4   KHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFV 63
           +H  W+QWQLLDS+LPTGGFAHS+GLEAA+Q+R+V+ PE+L++FV+ VL+NTGSLLLPFV
Sbjct: 37  QHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPFV 96

Query: 64  HSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETF 123
             A  SPD  TW K D+LL+AMLTNEV RKAS++QGSAL+RVAA VF+EI SL+ +R+TF
Sbjct: 97  CCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQTF 156

Query: 124 SGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQH 183
            G+  V FHHAPIFGLICGL+G+DS  +QRAY+F+TMRDVISAATRLNL+GPL A+VLQH
Sbjct: 157 LGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQH 216

Query: 184 QLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           Q+A  AE  ++KW +R VEEA Q+ PLL+ +QGCH Y+FSRLFC+
Sbjct: 217 QVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

[Close]

BLAST of MS010280 vs. ExPASy Swiss-Prot
Match: Q0E3L5 (Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2 SV=2)

HSP 1 Score: 322.0 bits (824), Expect = 5.4e-87
Identity = 153/225 (68.00%), Postives = 192/225 (85.33%), Query Frame = 0


Query: 4   KHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFV 63
           +H  W+QWQLLDS+LPTGGFAHS+GLEAA+Q+R+V+ PE+L++FV+ VL+NTGSLLLPFV
Sbjct: 37  QHSLWSQWQLLDSILPTGGFAHSYGLEAAMQSRMVNNPEELRSFVVQVLENTGSLLLPFV 96

Query: 64  HSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETF 123
             A  SPD  TW K D+LL+AMLTNEV RKAS++QGSAL+RVAA VF+EI SL+ +R+TF
Sbjct: 97  CCANKSPDAATWVKLDQLLEAMLTNEVSRKASMSQGSALLRVAASVFTEIQSLQDLRQTF 156

Query: 124 SGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQH 183
            G+  V FHHAPIFGLICGL+G+DS  +QRAY+F+TMRDVISAATRLNL+GPL A+VLQH
Sbjct: 157 LGSKIVSFHHAPIFGLICGLVGFDSETTQRAYMFVTMRDVISAATRLNLIGPLAASVLQH 216

Query: 184 QLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           Q+A  AE  ++KW +R VEEA Q+ PLL+ +QGCH Y+FSRLFC+
Sbjct: 217 QVAEDAERMVQKWKDRGVEEATQTSPLLDALQGCHAYMFSRLFCT 261

[Close]

BLAST of MS010280 vs. ExPASy Swiss-Prot
Match: B7K911 (Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=65393 GN=ureF PE=3 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 6.1e-14
Identity = 64/221 (28.96%), Postives = 99/221 (44.80%), Query Frame = 0


Query: 12  QLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDN----TGSLLLPFVHSAT 71
           QL DS  P+G F  S GLEA  Q   +   +DL+ F+  +L N    T  + L   +  +
Sbjct: 14  QLSDSFFPSGSFTLSHGLEALAQGEQIHSIKDLEIFLQILLHNKLGTTDLVALIHAYRGS 73

Query: 72  LSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFSGTG 131
              DL+  ++ D  L A    E  R+     G AL+ VA   + +       +ET  GT 
Sbjct: 74  KREDLDAVREADHQLFAQTLIEKNREMGRKSGRALLMVARETWQDTQLETLEKETAKGT- 133

Query: 132 AVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQLAL 191
            +   H  IF ++  + G     +  A+L   +  ++ AA RLN++G L A  +  +LA 
Sbjct: 134 -INGLHPIIFAVVGRVAGLSETDTGLAFLHSFITGLLGAAIRLNIIGHLQAQKILLKLAP 193

Query: 192 VAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
             E T +K     + E     PL++  Q  H  L  RLF +
Sbjct: 194 DLETTYQKATEINLNEMFSCTPLIDIAQMNHQNLDYRLFAN 232

[Close]

BLAST of MS010280 vs. ExPASy Swiss-Prot
Match: Q117Z4 (Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=ureF PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 2.3e-13
Identity = 63/221 (28.51%), Postives = 104/221 (47.06%), Query Frame = 0


Query: 12  QLLDSVLPTGGFAHSFGLEAAIQ-ARIVSQPEDLKTFVIHVLDNTG-SLLLPFVHS--AT 71
           QL DS  PTG F  S GLE  +Q  +I SQPE L    I + +  G S ++  +HS    
Sbjct: 14  QLSDSFFPTGSFTFSHGLETLVQTGKIQSQPEILYFLQILLRNKVGVSDVVALIHSYRGC 73

Query: 72  LSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFSGTG 131
            + D+E  +  DR+L         R+     G AL+ VA+  + +   LKT+    + +G
Sbjct: 74  KNGDIEAVRVADRMLFVQTAIAKNRETQRQSGRALLMVASSTWQD-ERLKTL-NIDAVSG 133

Query: 132 AVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQLAL 191
            +   H  IFG +  ++G D   +  A+L   + +++ AA RL ++G + A  +  QL  
Sbjct: 134 NIHCLHPVIFGAVTSVVGLDERNAVLAFLHGLVTNILGAAIRLGILGHIQAQQILLQLVP 193

Query: 192 VAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
             E      ++  +E+     P ++  Q  H  L  +LF +
Sbjct: 194 DIEAVWSTAVSMNLEQMWSCTPFIDIAQMQHPKLAHKLFAN 232

[Close]

BLAST of MS010280 vs. ExPASy TrEMBL
Match: A0A6J1DLZ1 (urease accessory protein F OS=Momordica charantia OX=3673 GN=LOC111021278 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 3.6e-126
Identity = 227/228 (99.56%), Postives = 227/228 (99.56%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL
Sbjct: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR
Sbjct: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV
Sbjct: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAED LKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS
Sbjct: 181 LQHQLALVAEDILKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 228

[Close]

BLAST of MS010280 vs. ExPASy TrEMBL
Match: A0A6J1G3M3 (urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 3.1e-114
Identity = 204/228 (89.47%), Postives = 215/228 (94.30%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+KNDR L+A+LTNEV RKAS+TQGSALMRVAA+VFSEI SLK MR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASVTQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 128 ETTTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAEDTLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 188 LQHQLALVAEDTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of MS010280 vs. ExPASy TrEMBL
Match: A0A6J1KB78 (urease accessory protein F OS=Cucurbita maxima OX=3661 GN=LOC111493898 PE=4 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.6e-113
Identity = 204/228 (89.47%), Postives = 214/228 (93.86%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD K   W+QWQLLDS+LPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIH+LDNTGSLLL
Sbjct: 8   MDDKQFHWSQWQLLDSILPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHLLDNTGSLLL 67

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHS TLSPDLETW+KNDR L+A+LTNEV RKASITQGSALMRVAA+VFSEI SLK MR
Sbjct: 68  PFVHSTTLSPDLETWEKNDRFLNAILTNEVSRKASITQGSALMRVAAIVFSEISSLKAMR 127

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           ET +GTGAV FHHAPIFGLICGLLGWDS MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 128 ETSTGTGAVSFHHAPIFGLICGLLGWDSTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 187

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLALVAE TLKKWMNRPVEEACQ+VPLLETVQGCH YLFSRLFCS
Sbjct: 188 LQHQLALVAEGTLKKWMNRPVEEACQTVPLLETVQGCHTYLFSRLFCS 235

[Close]

BLAST of MS010280 vs. ExPASy TrEMBL
Match: A0A0A0LJ08 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.8e-109
Identity = 194/228 (85.09%), Postives = 208/228 (91.23%), Query Frame = 0


Query: 1   MDGKHLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLL 60
           MD KH  W+QWQLLDS+LPTGGFAHSFGLEAAIQA+IVS P+DLKTFVIH+LDNTGSL L
Sbjct: 1   MDDKHCHWSQWQLLDSILPTGGFAHSFGLEAAIQAQIVSSPDDLKTFVIHLLDNTGSLFL 60

Query: 61  PFVHSATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMR 120
           PFVHSAT SPD ETW+KND LLDAMLTNEV RKAS+TQGSALMRV+A+VFSEIPSLK MR
Sbjct: 61  PFVHSATQSPDFETWKKNDMLLDAMLTNEVSRKASVTQGSALMRVSAIVFSEIPSLKAMR 120

Query: 121 ETFSGTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAV 180
           E   GTGAV FHHAPIFGLICGLLGWD  MSQRAYLFIT+RDVISAATRLNLVGPLGAAV
Sbjct: 121 ENLYGTGAVSFHHAPIFGLICGLLGWDGTMSQRAYLFITLRDVISAATRLNLVGPLGAAV 180

Query: 181 LQHQLALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           LQHQLA VAED LK+WMNRPVEEACQ+VPLLETVQGCH  LFS++FCS
Sbjct: 181 LQHQLAFVAEDILKRWMNRPVEEACQTVPLLETVQGCHSCLFSKMFCS 228

[Close]

BLAST of MS010280 vs. ExPASy TrEMBL
Match: A0A6P9EB16 (urease accessory protein F OS=Juglans regia OX=51240 GN=LOC109000628 PE=4 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 6.2e-102
Identity = 185/224 (82.59%), Postives = 202/224 (90.18%), Query Frame = 0


Query: 5   HLQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFVH 64
           HLQW+QWQL+DS+LPTGGFAHSFGLEAAIQARI+S PEDL+TFVIHVL+NTGSLLLPFV 
Sbjct: 89  HLQWSQWQLIDSILPTGGFAHSFGLEAAIQARIISGPEDLQTFVIHVLENTGSLLLPFVF 148

Query: 65  SATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFS 124
           SAT+SPDLETWQK DR LDAMLTNEVGRKASI QGSALMRVAA VFSE PSLK+MR+T  
Sbjct: 149 SATISPDLETWQKLDRTLDAMLTNEVGRKASIAQGSALMRVAAAVFSETPSLKSMRDTSV 208

Query: 125 GTGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQ 184
           G+G V FHHAPIFGL CGLLG DS  SQRAYLFITMRDVISAATRLNLVGP+GAAVLQHQ
Sbjct: 209 GSGVVSFHHAPIFGLTCGLLGLDSRTSQRAYLFITMRDVISAATRLNLVGPMGAAVLQHQ 268

Query: 185 LALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           + ++AE  L KWM+R VEEACQ+ PLL+TVQGCH YLFSRLFCS
Sbjct: 269 IVVLAEAILTKWMDRTVEEACQTTPLLDTVQGCHAYLFSRLFCS 312

[Close]

BLAST of MS010280 vs. TAIR 10
Match: AT1G21840.1 (urease accessory protein F )

HSP 1 Score: 334.3 bits (856), Expect = 7.5e-92
Identity = 156/223 (69.96%), Postives = 190/223 (85.20%), Query Frame = 0


Query: 6   LQWNQWQLLDSVLPTGGFAHSFGLEAAIQARIVSQPEDLKTFVIHVLDNTGSLLLPFVHS 65
           +QW+QWQLLDS+LPTGGFAHSFGLEAAIQ R+VS PEDL+T +IHVLDNT SLLLPFV+S
Sbjct: 18  MQWSQWQLLDSILPTGGFAHSFGLEAAIQTRLVSSPEDLETHIIHVLDNTASLLLPFVYS 77

Query: 66  ATLSPDLETWQKNDRLLDAMLTNEVGRKASITQGSALMRVAAVVFSEIPSLKTMRETFSG 125
           A  SPD+ETW K D +L+A LTN+V  KAS++QGSAL R+AA VF+E+P+LK +R+   G
Sbjct: 78  ALKSPDIETWHKLDGILNATLTNQVSSKASMSQGSALFRIAASVFTEVPNLKMIRDASLG 137

Query: 126 TGAVPFHHAPIFGLICGLLGWDSAMSQRAYLFITMRDVISAATRLNLVGPLGAAVLQHQL 185
           +  V FHHAPIFGL+CGLLG DS  SQRAYLF+T+RDV+SAATRLN+VGP+GA+V+QH++
Sbjct: 138 SKNVCFHHAPIFGLVCGLLGMDSETSQRAYLFVTLRDVLSAATRLNIVGPMGASVMQHRI 197

Query: 186 ALVAEDTLKKWMNRPVEEACQSVPLLETVQGCHMYLFSRLFCS 229
           A+V E  L+KWMNR   EACQ+ PLL+ VQGCH YLFSRLFCS
Sbjct: 198 AIVTETVLEKWMNREAGEACQTSPLLDVVQGCHGYLFSRLFCS 240

The following BLAST results are available for this feature:

BLAST of MS010280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153866.1	7.4e-126	99.56	urease accessory protein F [Momordica charantia] >XP_022153867.1 urease accessor...	[more]
XP_022946416.1	6.5e-114	89.47	urease accessory protein F [Cucurbita moschata]	[more]
XP_023545319.1	6.5e-114	89.47	urease accessory protein F [Cucurbita pepo subsp. pepo]	[more]
XP_022999571.1	3.2e-113	89.47	urease accessory protein F [Cucurbita maxima] >XP_022999572.1 urease accessory p...	[more]
KAG7030122.1	7.2e-113	88.60	Urease accessory protein F, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of MS010280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XHZ3	1.0e-90	69.96	Urease accessory protein F OS=Arabidopsis thaliana OX=3702 GN=UREF PE=2 SV=1	[more]
E0ZS46	5.4e-87	68.00	Urease accessory protein F OS=Oryza sativa subsp. indica OX=39946 GN=UREF PE=2 S...	[more]
Q0E3L5	5.4e-87	68.00	Urease accessory protein F OS=Oryza sativa subsp. japonica OX=39947 GN=UREF PE=2...	[more]
B7K911	6.1e-14	28.96	Urease accessory protein UreF OS=Gloeothece citriformis (strain PCC 7424) OX=653...	[more]
Q117Z4	2.3e-13	28.51	Urease accessory protein UreF OS=Trichodesmium erythraeum (strain IMS101) OX=203...	[more]

BLAST of MS010280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DLZ1	3.6e-126	99.56	urease accessory protein F OS=Momordica charantia OX=3673 GN=LOC111021278 PE=4 S...	[more]
A0A6J1G3M3	3.1e-114	89.47	urease accessory protein F OS=Cucurbita moschata OX=3662 GN=LOC111450480 PE=4 SV...	[more]
A0A6J1KB78	1.6e-113	89.47	urease accessory protein F OS=Cucurbita maxima OX=3661 GN=LOC111493898 PE=4 SV=1	[more]
A0A0A0LJ08	1.8e-109	85.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G033980 PE=4 SV=1	[more]
A0A6P9EB16	6.2e-102	82.59	urease accessory protein F OS=Juglans regia OX=51240 GN=LOC109000628 PE=4 SV=1	[more]

BLAST of MS010280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21840.1	7.5e-92	69.96	urease accessory protein F	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038277	UreF domain superfamily	GENE3D	1.10.4190.10	Urease accessory protein UreF	coord: 4..207 e-value: 1.2E-49 score: 171.1
IPR002639	Urease accessory protein UreF	PIRSF	PIRSF009467	Urease_acces_UreF	coord: 4..228 e-value: 9.1E-47 score: 157.7
IPR002639	Urease accessory protein UreF	PFAM	PF01730	UreF	coord: 38..185 e-value: 9.3E-19 score: 68.3
None	No IPR available	PANTHER	PTHR33620	UREASE ACCESSORY PROTEIN F	coord: 3..228

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010280.1	MS010280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0016151	nickel cation binding