MS010254 (gene) Bitter gourd (TR) v1

Overview


Name	MS010254
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	scaffold391: 1165300 .. 1166467 (+)
RNA-Seq Expression	MS010254
Synteny	MS010254

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGGTAATTAACTCGTAACCATTTCGTTTTTAGTTTTCATAACAAAATTCAAATAATAATAATTTTGTTGTTACCACCTTCTGTATGTGAATTAGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGGTAATGAAAATTCCTTCGTCTCTTTAATCTTTCTTTTCTCTTTTCTAAATCCTTAATTTGGTTCATCAATTTTTTAAATCGTTTTATTTTGGTATTTAAACTTCTAAATTTTCTATCTTTTCCCCAGACTTAATTACAACAACAACAAAAAAAAACCATTTTGGATCAACGTTGATATTTTTCAATTTAGCAAAGTGGATACCTTGATTTTTTAGCTTGTACATTAATGTGTTGACCTAGGTGAGGCATGTATTTGAGCTAAACAAGTGGACTAAGGAGAGTTTTGAGTAAGTAGTTGCATTTACTAAAATAACGTTTTAAATTTAAAATTTATAGACATTTTGAAAATTCAACTAAAATAAGATTGAAATTTACTTTTTGGGTTTTTAGGAAAAAGAAGACTTCCCTCCTAAATAAATGAGTAAATCTTACAAATATTTTTTATCTACTTAAAAATTTAAAAAAAAAAAAAACCCCCCTTTTTATTATGGTGTGGAATTGTTGTGTGTTTGTTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATT

mRNA sequence

ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATT

Coding sequence (CDS)

ATGGGCTCAAAATGGTGCGCGTTGGCGTTGGCGTTGGCGGTGGTGGTGCTTCTGGTGGGTTGTGTGAGGTCCAATATAGAGCAGGACAGGGCGGAGTGCGCGGACCAGGTGATTGGGCTGGCCACATGCCTGCCGTACGTGGGCGGGGCGGCCAAAGCGCCGACGCCGGACTGCTGCGGCGGCCTCAAGCTCGTGCTTCAAAAGAGTAAGAAATGTCTTTGCGTGTTGATAAAGGACCGTGATGACCCCACCCTCGGCCTCAAAGTTAACCTCACTCTTGCTCTTGGACTCCCCACTGCCTGCCATGCCCCTGCCGACATAGCCGAATGCGTCGACCTTCTGCATCTGCCGCCAAACTCCCAAGAAGCCAAGGACTTTCTGGGGTCTAAAAATAACACCACTGCTGCAACCACTCCTTCAGCAGCTGCAGCGAGTGGGAATTCTACAAAAAGTGGAGAAAATCCACAAGGGAAAAGTGATGGTGGAATGAGGAAACAATGGTTCGGCGTGGAGATATTCCTTTGGTTTTTCACTTCATTGCTTCTACTAATTTGTATT

Protein sequence

MGSKWCALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI

Homology

[Close]

BLAST of MS010254 vs. NCBI nr
Match: XP_022153758.1 (protein YLS3-like [Momordica charantia])

HSP 1 Score: 364.0 bits (933), Expect = 7.7e-97
Identity = 184/186 (98.92%), Postives = 184/186 (98.92%), Query Frame = 0


Query: 1   MGSKWCALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG 60
           MGSKWC  ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG
Sbjct: 1   MGSKWC--ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG 60

Query: 61  GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS 120
           GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS
Sbjct: 61  GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS 120

Query: 121 QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL 180
           QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL
Sbjct: 121 QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL 180

Query: 181 LLLICI 187
           LLLICI
Sbjct: 181 LLLICI 184

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BLAST of MS010254 vs. NCBI nr
Match: XP_038900291.1 (non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida])

HSP 1 Score: 242.7 bits (618), Expect = 2.6e-60
Identity = 123/178 (69.10%), Postives = 141/178 (79.21%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           L + + +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GL LVLQKS
Sbjct: 8   LLIVMALLFVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLNLVLQKS 67

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           +KCLC+LIKDRDDP+LGLKVNLTLALGLPTACH PADI +C+ LLHL PNS+EAKDFL +
Sbjct: 68  RKCLCILIKDRDDPSLGLKVNLTLALGLPTACHTPADIKDCIGLLHLSPNSKEAKDFLET 127

Query: 130 KNNTTAATTPSAAAASGNSTKSGENPQGKSDGG-MRKQWFGVEIFLWFFTSLLLLICI 187
               +  T+  A A SGNST   +N + K+DGG  R QW GVEIF+WF TS  LL CI
Sbjct: 128 NPKGSNTTSAPATAVSGNST---QNSEAKNDGGTKRNQWLGVEIFVWFSTSSFLLTCI 182

[Close]

BLAST of MS010254 vs. NCBI nr
Match: XP_011653558.1 (protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucumis sativus])

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-58
Identity = 122/179 (68.16%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           L  A+ +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GLKLVL KS
Sbjct: 9   LFAALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKS 68

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           +KCLCVLIKDRDDP+LGLKVNL+LALGLP+ACHAPA+I +CV LLHL PNS EAKDFLGS
Sbjct: 69  RKCLCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGS 128

Query: 130 KNN--TTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
            N+  T  +T   A   SG+S+   +N + K+DG  R QW GVE+F+WF TS  L  CI
Sbjct: 129 PNSKETNTSTPAHATPVSGSSS---QNSEVKNDGTKRNQWLGVEMFIWFITSSFLFTCI 184

[Close]

BLAST of MS010254 vs. NCBI nr
Match: XP_023512874.1 (protein YLS3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 234.6 bits (597), Expect = 7.0e-58
Identity = 123/178 (69.10%), Postives = 144/178 (80.90%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           ++ A+ +LLVG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAMALLLVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+G+
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGT 133

Query: 130 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
           K  NTTAA    A AA GNST   + P  K   GMR QW GVE+F+ F TS LL  CI
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPVEKG-SGMRNQWLGVEMFIGFLTSSLLFTCI 184

[Close]

BLAST of MS010254 vs. NCBI nr
Match: XP_022986686.1 (protein YLS3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 233.4 bits (594), Expect = 1.6e-57
Identity = 121/178 (67.98%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 130 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
           K  NTTAA    A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 184

[Close]

BLAST of MS010254 vs. ExPASy Swiss-Prot
Match: F4I082 (Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=3702 GN=LTPG6 PE=2 SV=2)

HSP 1 Score: 156.8 bits (395), Expect = 2.4e-37
Identity = 80/177 (45.20%), Postives = 117/177 (66.10%), Query Frame = 0


Query: 8   LALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 67
           L + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++
Sbjct: 8   LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 67

Query: 68  KSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF 127
           KS+KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F
Sbjct: 68  KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 127

Query: 128 --LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 182
             LG   +   +T+P+     G  T  G+    KS+G   K W GVE+ ++   SL+
Sbjct: 128 ENLGRIEDNYNSTSPTQIHKDG--TGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLI 182

[Close]

BLAST of MS010254 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 7.9e-36
Identity = 81/199 (40.70%), Postives = 118/199 (59.30%), Query Frame = 0


Query: 4   KWCALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLK 63
           K  A A+AL +V ++V    ++  +D+ EC  Q++G+ATCLPYV G AK+PTPDCC GLK
Sbjct: 7   KVMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLK 66

Query: 64  LVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEA 123
            V+    KCLC++I++R+DP LGL+VN++LAL LP+ CHA ADI +C  LLHL PNS +A
Sbjct: 67  QVINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDA 126

Query: 124 KDF----------------LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQW 183
           + F                 GS +  T+ ++   ++A  NS ++   P         KQW
Sbjct: 127 QVFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQW 186

Query: 184 FGVEIFLWFFTSLLLLICI 187
            G+E+   FF    + I +
Sbjct: 187 LGLEVVAHFFVIFYIFILV 205

[Close]

BLAST of MS010254 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 1.3e-35
Identity = 83/196 (42.35%), Postives = 121/196 (61.73%), Query Frame = 0


Query: 7   ALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVL 66
           A A+AL VV ++V     +  +D+ EC +Q++G+ATCLPYV G AK+PTPDCC GLK VL
Sbjct: 10  ATAIALIVVAMVVAAA-DDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVL 69

Query: 67  QKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF 126
             +KKCLCV+I+DR+DP LGL++N++LAL LP+ CHA AD+ +C  LLHL PNS +A+ F
Sbjct: 70  NSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVF 129

Query: 127 ------LGSKNNTTAAT---------TPSAAAASGNSTKSGENPQGKSDG-GMRKQWFGV 186
                 L      +A T         +P++ +  GN++    +  G++      KQW G+
Sbjct: 130 YQLAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGL 189

[Close]

BLAST of MS010254 vs. ExPASy Swiss-Prot
Match: Q9M2G1 (Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3702 GN=LTPG22 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 9.6e-26
Identity = 69/164 (42.07%), Postives = 96/164 (58.54%), Query Frame = 0


Query: 8   LALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 67
           LAL + V V+ +G VRS + QD   C D +  L +CLP+V   AKAP   CC  LK+ + 
Sbjct: 14  LALCITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKID 73

Query: 68  K--SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKD 127
           K  ++KCLC L+KDRDDP LG KV+   A+ LP+ACH PA+I++C DLLHL P+S  A  
Sbjct: 74  KGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS-PASQ 133

Query: 128 FLGSKNNTTAATTPSAAAASGNSTKSGENPQ-GKSDGGMRKQWF 169
                  +++ T    A ++ +S K  +N Q G    G    W+
Sbjct: 134 IFKQFTESSSQTVGHKAVSTSSSIKGRDNKQFGLMMAGALSIWY 175

[Close]

BLAST of MS010254 vs. ExPASy Swiss-Prot
Match: F4HZB9 (Non-specific lipid transfer protein GPI-anchored 19 OS=Arabidopsis thaliana OX=3702 GN=LTPG19 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-09
Identity = 51/177 (28.81%), Postives = 77/177 (43.50%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           LAL +   L G         +A C D +  L+ CL Y+ G + +P+  CC     V+Q S
Sbjct: 6   LALVIATFLYG----GATTVQAGCRDTLTSLSPCLYYLNGGSSSPSWSCCRQFSTVVQSS 65

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
            +CLC ++   +    G K N TLAL LPTAC+         +     P +  A   +GS
Sbjct: 66  PECLCSVVNSNESSFYGFKFNRTLALNLPTACNVQTPSPSLCNTGGNVPTTLPANTPVGS 125

Query: 130 KNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
                +A +PS   +  N T SG      S+    K      + +  F S+ L++ I
Sbjct: 126 PR---SAPSPSGTTSPAN-TPSGSKKFPLSNESSSKS----NVIILSFVSIALVLAI 170

[Close]

BLAST of MS010254 vs. ExPASy TrEMBL
Match: A0A6J1DIB7 (protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111021200 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.7e-97
Identity = 184/186 (98.92%), Postives = 184/186 (98.92%), Query Frame = 0


Query: 1   MGSKWCALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG 60
           MGSKWC  ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG
Sbjct: 1   MGSKWC--ALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCG 60

Query: 61  GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS 120
           GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS
Sbjct: 61  GLKLVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNS 120

Query: 121 QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL 180
           QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL
Sbjct: 121 QEAKDFLGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSL 180

Query: 181 LLLICI 187
           LLLICI
Sbjct: 181 LLLICI 184

[Close]

BLAST of MS010254 vs. ExPASy TrEMBL
Match: A0A0A0KZ48 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 6.9e-59
Identity = 122/179 (68.16%), Postives = 141/179 (78.77%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           L  A+ +L VG V SNI+QDRAECADQV+GLATCLPYVGG AKAPTPDCC GLKLVL KS
Sbjct: 9   LFAALALLSVGFVSSNIDQDRAECADQVVGLATCLPYVGGEAKAPTPDCCSGLKLVLDKS 68

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           +KCLCVLIKDRDDP+LGLKVNL+LALGLP+ACHAPA+I +CV LLHL PNS EAKDFLGS
Sbjct: 69  RKCLCVLIKDRDDPSLGLKVNLSLALGLPSACHAPANIKDCVGLLHLSPNSPEAKDFLGS 128

Query: 130 KNN--TTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
            N+  T  +T   A   SG+S+   +N + K+DG  R QW GVE+F+WF TS  L  CI
Sbjct: 129 PNSKETNTSTPAHATPVSGSSS---QNSEVKNDGTKRNQWLGVEMFIWFITSSFLFTCI 184

[Close]

BLAST of MS010254 vs. ExPASy TrEMBL
Match: A0A6J1JBX4 (protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 7.6e-58
Identity = 121/178 (67.98%), Postives = 142/178 (79.78%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 130 KN-NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
           K  NTTAA    A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 KGANTTAA---PATAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 184

[Close]

BLAST of MS010254 vs. ExPASy TrEMBL
Match: A0A6J1J894 (protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.7e-57
Identity = 118/177 (66.67%), Postives = 140/177 (79.10%), Query Frame = 0


Query: 10  LALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKS 69
           ++ A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KS
Sbjct: 14  ISCAIALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKS 73

Query: 70  KKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGS 129
           KKCLCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GS
Sbjct: 74  KKCLCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGS 133

Query: 130 KNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
           K     A T +A AA GNST   + P  K   GMR QW G E+F+ F TS LL  C+
Sbjct: 134 K----GANTTAAPAAPGNST---QTPMEKG-SGMRNQWLGAEMFIAFLTSSLLFTCV 182

[Close]

BLAST of MS010254 vs. ExPASy TrEMBL
Match: A0A6J1FVR4 (protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.2e-57
Identity = 123/175 (70.29%), Postives = 141/175 (80.57%), Query Frame = 0


Query: 13  AVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQKSKKC 72
           A+ +L VG V SN++QDRAECADQV+GLATCLPYVGG A+APTPDCC GLK+VL+KSKKC
Sbjct: 17  AMALLFVGFVSSNVDQDRAECADQVVGLATCLPYVGGEARAPTPDCCSGLKVVLEKSKKC 76

Query: 73  LCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDFLGSKN- 132
           LCVLIKDR+DP+LGLKVNLTLALGLP ACHAPA+I +C+ LLHL PNSQ+AKDF+GSK  
Sbjct: 77  LCVLIKDRNDPSLGLKVNLTLALGLPNACHAPANIQDCIGLLHLSPNSQDAKDFMGSKGA 136

Query: 133 NTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLLLLICI 187
           NTTAA    A AA GNST+S   P  K   GMR QW  VE+F+ F TS LL  CI
Sbjct: 137 NTTAA---PAFAAPGNSTQS---PMEKG-SGMRNQWSSVEMFIGFLTSSLLFTCI 184

[Close]

BLAST of MS010254 vs. TAIR 10
Match: AT1G55260.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 1.7e-38
Identity = 80/177 (45.20%), Postives = 117/177 (66.10%), Query Frame = 0


Query: 8   LALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 67
           L + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 68  KSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF 127
           KS+KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 170

Query: 128 --LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 182
             LG   +   +T+P+     G  T  G+    KS+G   K W GVE+ ++   SL+
Sbjct: 171 ENLGRIEDNYNSTSPTQIHKDG--TGGGKAEPVKSNGWKEKSWLGVELLIYLLVSLI 225

[Close]

BLAST of MS010254 vs. TAIR 10
Match: AT1G55260.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 5.1e-38
Identity = 79/177 (44.63%), Postives = 116/177 (65.54%), Query Frame = 0


Query: 8   LALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 67
           L + + +  +L+G   S++ QDR EC +Q+I L+TC+PYVGG AKAPT DCC G   V++
Sbjct: 51  LFITIVITSMLLGFGNSDLAQDREECTNQLIELSTCIPYVGGDAKAPTKDCCAGFGQVIR 110

Query: 68  KSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPA-DIAECVDLLHLPPNSQEAKDF 127
           KS+KC+C+L++D+DDP LG+K+N TLA  LP+ACH  A +I +C+ +LHLP NS  AK+F
Sbjct: 111 KSEKCVCILVRDKDDPQLGIKINATLAAHLPSACHITAPNITDCISILHLPRNSTLAKEF 170

Query: 128 --LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQWFGVEIFLWFFTSLL 182
             LG   +   +T+P+     G     G+    KS+G   K W GVE+ ++   SL+
Sbjct: 171 ENLGRIEDNYNSTSPTQIHKGG-----GKAEPVKSNGWKEKSWLGVELLIYLLVSLI 222

[Close]

BLAST of MS010254 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 151.8 bits (382), Expect = 5.6e-37
Identity = 81/199 (40.70%), Postives = 118/199 (59.30%), Query Frame = 0


Query: 4   KWCALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLK 63
           K  A A+AL +V ++V    ++  +D+ EC  Q++G+ATCLPYV G AK+PTPDCC GLK
Sbjct: 7   KVMATAIALIMVAMVVDAAGADKGKDKEECTAQLVGMATCLPYVQGKAKSPTPDCCSGLK 66

Query: 64  LVLQKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEA 123
            V+    KCLC++I++R+DP LGL+VN++LAL LP+ CHA ADI +C  LLHL PNS +A
Sbjct: 67  QVINSDMKCLCMIIQERNDPDLGLQVNVSLALALPSVCHATADITKCPALLHLDPNSPDA 126

Query: 124 KDF----------------LGSKNNTTAATTPSAAAASGNSTKSGENPQGKSDGGMRKQW 183
           + F                 GS +  T+ ++   ++A  NS ++   P         KQW
Sbjct: 127 QVFYQLAKGLNETVSASAPTGSASEPTSMSSTPGSSAGNNSGRTTSVPGTNHAQSFSKQW 186

Query: 184 FGVEIFLWFFTSLLLLICI 187
            G+E+   FF    + I +
Sbjct: 187 LGLEVVAHFFVIFYIFILV 205

[Close]

BLAST of MS010254 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 151.0 bits (380), Expect = 9.5e-37
Identity = 83/196 (42.35%), Postives = 121/196 (61.73%), Query Frame = 0


Query: 7   ALALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVL 66
           A A+AL VV ++V     +  +D+ EC +Q++G+ATCLPYV G AK+PTPDCC GLK VL
Sbjct: 10  ATAIALIVVAMVVAAA-DDKTKDKEECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQVL 69

Query: 67  QKSKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKDF 126
             +KKCLCV+I+DR+DP LGL++N++LAL LP+ CHA AD+ +C  LLHL PNS +A+ F
Sbjct: 70  NSNKKCLCVIIQDRNDPDLGLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQVF 129

Query: 127 ------LGSKNNTTAAT---------TPSAAAASGNSTKSGENPQGKSDG-GMRKQWFGV 186
                 L      +A T         +P++ +  GN++    +  G++      KQW G+
Sbjct: 130 YQLAKGLNKTGPASAPTGSSPGPISISPTSGSDDGNNSGRTTSVPGRNHAQSFYKQWLGL 189

[Close]

BLAST of MS010254 vs. TAIR 10
Match: AT3G58550.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 118.2 bits (295), Expect = 6.9e-27
Identity = 69/164 (42.07%), Postives = 96/164 (58.54%), Query Frame = 0


Query: 8   LALALAVVVLLVGCVRSNIEQDRAECADQVIGLATCLPYVGGAAKAPTPDCCGGLKLVLQ 67
           LAL + V V+ +G VRS + QD   C D +  L +CLP+V   AKAP   CC  LK+ + 
Sbjct: 14  LALCITVAVMFLG-VRSELSQDIKGCQDAMSDLYSCLPFVTNKAKAPDSTCCSTLKVKID 73

Query: 68  K--SKKCLCVLIKDRDDPTLGLKVNLTLALGLPTACHAPADIAECVDLLHLPPNSQEAKD 127
           K  ++KCLC L+KDRDDP LG KV+   A+ LP+ACH PA+I++C DLLHL P+S  A  
Sbjct: 74  KGQTRKCLCTLVKDRDDPGLGFKVDANRAMSLPSACHVPANISQCPDLLHLLPDS-PASQ 133

Query: 128 FLGSKNNTTAATTPSAAAASGNSTKSGENPQ-GKSDGGMRKQWF 169
                  +++ T    A ++ +S K  +N Q G    G    W+
Sbjct: 134 IFKQFTESSSQTVGHKAVSTSSSIKGRDNKQFGLMMAGALSIWY 175

The following BLAST results are available for this feature:

BLAST of MS010254 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153758.1	7.7e-97	98.92	protein YLS3-like [Momordica charantia]	[more]
XP_038900291.1	2.6e-60	69.10	non-specific lipid transfer protein GPI-anchored 6 [Benincasa hispida]	[more]
XP_011653558.1	1.4e-58	68.16	protein YLS3 [Cucumis sativus] >KGN54079.1 hypothetical protein Csa_018100 [Cucu...	[more]
XP_023512874.1	7.0e-58	69.10	protein YLS3-like [Cucurbita pepo subsp. pepo]	[more]
XP_022986686.1	1.6e-57	67.98	protein YLS3-like isoform X1 [Cucurbita maxima]	[more]

BLAST of MS010254 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I082	2.4e-37	45.20	Non-specific lipid transfer protein GPI-anchored 6 OS=Arabidopsis thaliana OX=37...	[more]
O64864	7.9e-36	40.70	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
O64865	1.3e-35	42.35	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
Q9M2G1	9.6e-26	42.07	Non-specific lipid transfer protein GPI-anchored 22 OS=Arabidopsis thaliana OX=3...	[more]
F4HZB9	3.7e-09	28.81	Non-specific lipid transfer protein GPI-anchored 19 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of MS010254 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DIB7	3.7e-97	98.92	protein YLS3-like OS=Momordica charantia OX=3673 GN=LOC111021200 PE=4 SV=1	[more]
A0A0A0KZ48	6.9e-59	68.16	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G280560 PE=4 SV...	[more]
A0A6J1JBX4	7.6e-58	67.98	protein YLS3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV...	[more]
A0A6J1J894	1.7e-57	66.67	protein YLS3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484364 PE=4 SV...	[more]
A0A6J1FVR4	2.2e-57	70.29	protein YLS3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448546 PE=4 ...	[more]

BLAST of MS010254 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G55260.1	1.7e-38	45.20	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G55260.2	5.1e-38	44.63	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	5.6e-37	40.70	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44300.1	9.5e-37	42.35	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G58550.1	6.9e-27	42.07	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 33..110 e-value: 0.0075 score: 25.5
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 16..110 e-value: 2.3E-12 score: 46.9
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 30..108 e-value: 3.6E-13 score: 51.2
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 32..110
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 136..160
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 136..156
None	No IPR available	PANTHER	PTHR33044:SF194	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 9..176
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..21 score: 5.0
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 40..102 e-value: 7.4317E-17 score: 69.3778
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 9..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010254.1	MS010254.1	mRNA