MS010245 (gene) Bitter gourd (TR) v1

Overview
NameMS010245
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionTransmembrane protein
Locationscaffold391: 1112322 .. 1112702 (-)
RNA-Seq ExpressionMS010245
SyntenyMS010245
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCGATACTACAACAACTGGTATTCTTATTATTGGGGGGAATACTTTAACTTGACGTTCCCTCCGGTGGAGCTGTGCGTGTTCGGGGTGATCCTGGTGCTGGTGATGGGCTTCTCATGGTACATCTTCTACGACTCCATTCTCCAGGAGTTGATCGTCCAGCTCAAGCTGTTCCTCCTCACCGTCCCCCTGCTTCTGCTCCTCCTCCTCCACCTCCTCTCTCTCGGCCTCCCCTTCCTCCTCCCTCTGCCGGAGCAGGACTCCCTCCACCGCGCCGGCGCCTCCCCGTGGGGCGTCGCTCTCCTCCTCATCTTTCTTCTCTACCTCATCTCTCACCAGTCCCACTACCACCAACGCTGGTTCCCTTTCGGATACAGG

mRNA sequence

ATGGCTCGATACTACAACAACTGGTATTCTTATTATTGGGGGGAATACTTTAACTTGACGTTCCCTCCGGTGGAGCTGTGCGTGTTCGGGGTGATCCTGGTGCTGGTGATGGGCTTCTCATGGTACATCTTCTACGACTCCATTCTCCAGGAGTTGATCGTCCAGCTCAAGCTGTTCCTCCTCACCGTCCCCCTGCTTCTGCTCCTCCTCCTCCACCTCCTCTCTCTCGGCCTCCCCTTCCTCCTCCCTCTGCCGGAGCAGGACTCCCTCCACCGCGCCGGCGCCTCCCCGTGGGGCGTCGCTCTCCTCCTCATCTTTCTTCTCTACCTCATCTCTCACCAGTCCCACTACCACCAACGCTGGTTCCCTTTCGGATACAGG

Coding sequence (CDS)

ATGGCTCGATACTACAACAACTGGTATTCTTATTATTGGGGGGAATACTTTAACTTGACGTTCCCTCCGGTGGAGCTGTGCGTGTTCGGGGTGATCCTGGTGCTGGTGATGGGCTTCTCATGGTACATCTTCTACGACTCCATTCTCCAGGAGTTGATCGTCCAGCTCAAGCTGTTCCTCCTCACCGTCCCCCTGCTTCTGCTCCTCCTCCTCCACCTCCTCTCTCTCGGCCTCCCCTTCCTCCTCCCTCTGCCGGAGCAGGACTCCCTCCACCGCGCCGGCGCCTCCCCGTGGGGCGTCGCTCTCCTCCTCATCTTTCTTCTCTACCTCATCTCTCACCAGTCCCACTACCACCAACGCTGGTTCCCTTTCGGATACAGG

Protein sequence

MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFLLTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQRWFPFGYR
Homology
BLAST of MS010245 vs. NCBI nr
Match: KAG6570356.1 (hypothetical protein SDJN03_29271, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010237.1 hypothetical protein SDJN02_27029, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 187.2 bits (474), Expect = 8.8e-44
Identity = 99/127 (77.95%), Postives = 114/127 (89.76%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYY  ++ Y + EY  LT PP++LCVFG IL+LVM FSWYIFYDS L+++I QLKLFL
Sbjct: 1   MARYY-YYHRYPYLEY--LTLPPLQLCVFGGILLLVMAFSWYIFYDSFLEDIIFQLKLFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           LTVPLLLLLLLHLLSLGLPFL+PLPEQ+SLHRAG SPWGVA+LL+FLLY+ISHQSHYH+R
Sbjct: 61  LTVPLLLLLLLHLLSLGLPFLVPLPEQNSLHRAGGSPWGVAILLVFLLYVISHQSHYHRR 120

Query: 121 WFPFGYR 128
           WFPFGYR
Sbjct: 121 WFPFGYR 124

BLAST of MS010245 vs. NCBI nr
Match: PON93547.1 (Transmembrane protein [Trema orientale])

HSP 1 Score: 148.3 bits (373), Expect = 4.5e-32
Identity = 78/124 (62.90%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYYNN+YSY+  +YF++   P+ LC F +IL  ++GFSWYI Y++  ++LI Q+KLFL
Sbjct: 1   MARYYNNYYSYW--DYFSI---PLHLCFFMLILFFMLGFSWYINYENKFEDLITQVKLFL 60

Query: 61  LTVPLLLLLLLHLLSL-GLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQ 120
           + VPLLLLL++H LS  G+PFL+PLPEQDSLHRAG SPWGV LLL+FLL++IS+QS +H+
Sbjct: 61  MIVPLLLLLVVHCLSSGGVPFLIPLPEQDSLHRAGGSPWGVGLLLVFLLFMISYQSSFHE 119

Query: 121 RWFP 124
           RWFP
Sbjct: 121 RWFP 119

BLAST of MS010245 vs. NCBI nr
Match: PON65170.1 (Transmembrane protein [Parasponia andersonii])

HSP 1 Score: 146.0 bits (367), Expect = 2.2e-31
Identity = 78/124 (62.90%), Postives = 102/124 (82.26%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYYNN+YSY+  +YF++   P+ LC F +IL  ++GFSWYI Y++  ++LI Q+KLFL
Sbjct: 1   MARYYNNYYSYW--DYFSI---PLHLCFFILILFFMLGFSWYINYENKFEDLITQVKLFL 60

Query: 61  LTVPLLLLLLLHLLSL-GLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQ 120
           + VPLLLLL++H LS  GL FL+PLPEQDSLHRAG SPWGV LLL+FLL++IS+QS +H+
Sbjct: 61  MIVPLLLLLVVHCLSSGGLQFLIPLPEQDSLHRAGGSPWGVGLLLVFLLFMISYQSSFHE 119

Query: 121 RWFP 124
           RWFP
Sbjct: 121 RWFP 119

BLAST of MS010245 vs. NCBI nr
Match: XP_007014761.1 (PREDICTED: uncharacterized protein LOC18589643 [Theobroma cacao] >EOY32380.1 Uncharacterized protein TCM_040264 [Theobroma cacao])

HSP 1 Score: 138.7 bits (348), Expect = 3.6e-29
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYY + +  Y      L+ PP+ LC F  IL  V+GFSWYI Y+S+ ++ I QLK FL
Sbjct: 1   MARYYRDSFLDY------LSLPPLHLCFFVSILFFVLGFSWYINYESMFEDFINQLKFFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           +  P++LLLL+H  S  +PFL+PLPEQDSLHR G SPWGVALLL+FLLY+IS+QS +H+R
Sbjct: 61  MLSPVVLLLLIHCFSGSVPFLIPLPEQDSLHRTGGSPWGVALLLVFLLYMISYQSSFHER 119

Query: 121 WFPFG 126
           WFP G
Sbjct: 121 WFPLG 119

BLAST of MS010245 vs. NCBI nr
Match: XP_022756288.1 (uncharacterized protein LOC111304026 [Durio zibethinus])

HSP 1 Score: 137.9 bits (346), Expect = 6.1e-29
Identity = 75/123 (60.98%), Postives = 96/123 (78.05%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYY     Y + +Y  L+ PPV LC+F  IL  V+GFSWYI Y+S+ ++ I QLK FL
Sbjct: 1   MARYYR----YSFLDY--LSPPPVHLCLFVFILFFVLGFSWYINYESMFEDFINQLKFFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           +  PL+LL+L+H LS  +PFL+PLPEQDSLHRAG SPWGVAL+L+FLLY+IS+QS +H+R
Sbjct: 61  MLTPLVLLVLVHCLSGRVPFLIPLPEQDSLHRAGGSPWGVALVLVFLLYMISYQSSFHER 117

Query: 121 WFP 124
           WFP
Sbjct: 121 WFP 117

BLAST of MS010245 vs. ExPASy TrEMBL
Match: A0A0A0KZ55 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280660 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.5e-41
Identity = 96/127 (75.59%), Postives = 107/127 (84.25%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MAR YN +Y Y + EY  LT PP++LCVF VIL++VM FSWY FY S L++ I QLKLFL
Sbjct: 1   MAR-YNYYYRYPYLEY--LTLPPLQLCVFVVILLVVMAFSWYFFYYSFLEDFIFQLKLFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           LTVPLLLLLLLHLLS G  FLLPLPEQDSLHRAG SPWGVA+LL+F LY+ISHQSHYHQR
Sbjct: 61  LTVPLLLLLLLHLLSFGFSFLLPLPEQDSLHRAGGSPWGVAILLVFFLYVISHQSHYHQR 120

Query: 121 WFPFGYR 128
           WFPFGYR
Sbjct: 121 WFPFGYR 124

BLAST of MS010245 vs. ExPASy TrEMBL
Match: A0A2P5F6Z2 (Transmembrane protein OS=Trema orientale OX=63057 GN=TorRG33x02_105310 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.2e-32
Identity = 78/124 (62.90%), Postives = 103/124 (83.06%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYYNN+YSY+  +YF++   P+ LC F +IL  ++GFSWYI Y++  ++LI Q+KLFL
Sbjct: 1   MARYYNNYYSYW--DYFSI---PLHLCFFMLILFFMLGFSWYINYENKFEDLITQVKLFL 60

Query: 61  LTVPLLLLLLLHLLSL-GLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQ 120
           + VPLLLLL++H LS  G+PFL+PLPEQDSLHRAG SPWGV LLL+FLL++IS+QS +H+
Sbjct: 61  MIVPLLLLLVVHCLSSGGVPFLIPLPEQDSLHRAGGSPWGVGLLLVFLLFMISYQSSFHE 119

Query: 121 RWFP 124
           RWFP
Sbjct: 121 RWFP 119

BLAST of MS010245 vs. ExPASy TrEMBL
Match: A0A2P5CVU8 (Transmembrane protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_118760 PE=4 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.1e-31
Identity = 78/124 (62.90%), Postives = 102/124 (82.26%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYYNN+YSY+  +YF++   P+ LC F +IL  ++GFSWYI Y++  ++LI Q+KLFL
Sbjct: 1   MARYYNNYYSYW--DYFSI---PLHLCFFILILFFMLGFSWYINYENKFEDLITQVKLFL 60

Query: 61  LTVPLLLLLLLHLLSL-GLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQ 120
           + VPLLLLL++H LS  GL FL+PLPEQDSLHRAG SPWGV LLL+FLL++IS+QS +H+
Sbjct: 61  MIVPLLLLLVVHCLSSGGLQFLIPLPEQDSLHRAGGSPWGVGLLLVFLLFMISYQSSFHE 119

Query: 121 RWFP 124
           RWFP
Sbjct: 121 RWFP 119

BLAST of MS010245 vs. ExPASy TrEMBL
Match: A0A061GR99 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_040264 PE=4 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.7e-29
Identity = 73/125 (58.40%), Postives = 93/125 (74.40%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYY + +  Y      L+ PP+ LC F  IL  V+GFSWYI Y+S+ ++ I QLK FL
Sbjct: 1   MARYYRDSFLDY------LSLPPLHLCFFVSILFFVLGFSWYINYESMFEDFINQLKFFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           +  P++LLLL+H  S  +PFL+PLPEQDSLHR G SPWGVALLL+FLLY+IS+QS +H+R
Sbjct: 61  MLSPVVLLLLIHCFSGSVPFLIPLPEQDSLHRTGGSPWGVALLLVFLLYMISYQSSFHER 119

Query: 121 WFPFG 126
           WFP G
Sbjct: 121 WFPLG 119

BLAST of MS010245 vs. ExPASy TrEMBL
Match: A0A6P5ZU06 (uncharacterized protein LOC111304026 OS=Durio zibethinus OX=66656 GN=LOC111304026 PE=4 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.0e-29
Identity = 75/123 (60.98%), Postives = 96/123 (78.05%), Query Frame = 0

Query: 1   MARYYNNWYSYYWGEYFNLTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFL 60
           MARYY     Y + +Y  L+ PPV LC+F  IL  V+GFSWYI Y+S+ ++ I QLK FL
Sbjct: 1   MARYYR----YSFLDY--LSPPPVHLCLFVFILFFVLGFSWYINYESMFEDFINQLKFFL 60

Query: 61  LTVPLLLLLLLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQR 120
           +  PL+LL+L+H LS  +PFL+PLPEQDSLHRAG SPWGVAL+L+FLLY+IS+QS +H+R
Sbjct: 61  MLTPLVLLVLVHCLSGRVPFLIPLPEQDSLHRAGGSPWGVALVLVFLLYMISYQSSFHER 117

Query: 121 WFP 124
           WFP
Sbjct: 121 WFP 117

BLAST of MS010245 vs. TAIR 10
Match: AT5G19875.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response to oxidative stress; LOCATED IN: endomembrane system; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G31940.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 110.2 bits (274), Expect = 1.3e-24
Identity = 54/116 (46.55%), Postives = 82/116 (70.69%), Query Frame = 0

Query: 11  YYWGEYFN-LTFPPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFLLTVPLLLLL 70
           YY   Y + L+ P   LC   +++  V+ F+WY+ ++SI+++ +  LKL  +  PL LLL
Sbjct: 6   YYGSSYLDYLSLPNPHLCFLFIVVFFVLSFTWYLNFESIIEDTLDHLKLVFIFTPLFLLL 65

Query: 71  LLHLLSLGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQRWFPFG 126
           L+H  S GL F +P PEQDS+HRAG+SPWGVA +L+ +L+++S+QS + +RWFPFG
Sbjct: 66  LVHFFSGGLSFYVPWPEQDSIHRAGSSPWGVAAVLVLILFMVSYQSDFQERWFPFG 121

BLAST of MS010245 vs. TAIR 10
Match: AT2G31940.1 (unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 16 plant structures; EXPRESSED DURING: 6 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G19875.1); Has 227 Blast hits to 227 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 227; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 87.8 bits (216), Expect = 6.8e-18
Identity = 48/107 (44.86%), Postives = 74/107 (69.16%), Query Frame = 0

Query: 23  PVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFLLTVPLLLLLLLHLLS----LG- 82
           P+ LCVF +IL++ +  SWY  Y+ +++    Q KL L+  PLLLLL +H LS    +G 
Sbjct: 12  PLHLCVFVLILLMFVTISWYASYEPVIEGFTYQFKLALMASPLLLLLAVHFLSDDQGVGG 71

Query: 83  -LPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQRWFP 124
            +  L+ L E++SL+RAG +PWGVA +L+FL +++S+QS + +RWFP
Sbjct: 72  MMTSLIHLNERESLYRAGGTPWGVAFMLVFLFFMVSYQSQFQERWFP 118

BLAST of MS010245 vs. TAIR 10
Match: AT5G42146.1 (unknown protein; Has 98 Blast hits to 98 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 98; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 51.2 bits (121), Expect = 7.0e-07
Identity = 37/108 (34.26%), Postives = 57/108 (52.78%), Query Frame = 0

Query: 22  PPVELCVFGVILVLVMGFSWYIFYDSILQELIVQLKLFLLTVPLLLLLLL------HLLS 81
           PP+ L     I+ L++  S Y  Y   +++    LKLF+L +P+L + +L      H L 
Sbjct: 28  PPLTLLALLAIISLLLFLSSYPRYKYEVEKTAANLKLFMLFLPILFVFVLVSLSFVHRLL 87

Query: 82  LGLPFLLPLPEQDSLHRAGASPWGVALLLIFLLYLISHQSHYHQRWFP 124
               + +   +  SL   G  PWGV L+LI LL L+S QS++H  W+P
Sbjct: 88  FKSSYSVRANQAKSLFGEGNFPWGVLLMLILLLLLVSKQSYFHSLWYP 135

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6570356.18.8e-4477.95hypothetical protein SDJN03_29271, partial [Cucurbita argyrosperma subsp. sorori... [more]
PON93547.14.5e-3262.90Transmembrane protein [Trema orientale][more]
PON65170.12.2e-3162.90Transmembrane protein [Parasponia andersonii][more]
XP_007014761.13.6e-2958.40PREDICTED: uncharacterized protein LOC18589643 [Theobroma cacao] >EOY32380.1 Unc... [more]
XP_022756288.16.1e-2960.98uncharacterized protein LOC111304026 [Durio zibethinus][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A0A0KZ551.5e-4175.59Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280660 PE=4 SV=1[more]
A0A2P5F6Z22.2e-3262.90Transmembrane protein OS=Trema orientale OX=63057 GN=TorRG33x02_105310 PE=4 SV=1[more]
A0A2P5CVU81.1e-3162.90Transmembrane protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_118760 PE=4... [more]
A0A061GR991.7e-2958.40Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_040264 PE=4 SV=1[more]
A0A6P5ZU063.0e-2960.98uncharacterized protein LOC111304026 OS=Durio zibethinus OX=66656 GN=LOC11130402... [more]
Match NameE-valueIdentityDescription
AT5G19875.11.3e-2446.55unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: response... [more]
AT2G31940.16.8e-1844.86unknown protein; INVOLVED IN: biological_process unknown; LOCATED IN: endomembra... [more]
AT5G42146.17.0e-0734.26unknown protein; Has 98 Blast hits to 98 proteins in 12 species: Archae - 0; Bac... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR33306:SF24EXPRESSED PROTEINcoord: 1..125
NoneNo IPR availablePANTHERPTHR33306EXPRESSED PROTEIN-RELATED-RELATEDcoord: 1..125

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010245.1MS010245.1mRNA