MS010241 (gene) Bitter gourd (TR) v1

Overview


Name	MS010241
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	RING-type E3 ubiquitin transferase
Location	scaffold391: 1092063 .. 1093477 (+)
RNA-Seq Expression	MS010241
Synteny	MS010241

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATGAGTATTGTAGAATTCAGAGAGAAGGAGATGTACCAGCAGAAGAAGTTAAGGGAAGAACATACTTCTTCTTCTGGGGCCAGCTTCTGCAAGGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAAGCAGCAACTTCTCGTCCAGAGTTCAAAAGCGTCTCTTCCAGGAAATCTTCCACTGGTTTTGGCAGGAATTGTAACAATGTACATGAGCTACTAGATTGCACTGTTTGCATGGATTTAATGCACCCTCCAATTTACCAGGTACCATATTCTAATTGTGATGAATTTCAGAACTTACAATGCTTGCCCATCCAAGAAGGAATCTTGAAACAAATGCCATAAGTTTGTGACCTTGAATCAAAGTTAGAGGCCTCGTGCATTATTAAGAAATCCTTCTGTCCCCGAAAATAGTTTTCAATGAAGTATAAAAAGCGTTCTAGCTGGCTTCACTCTACCTTCAAATGGAAGACTATTTTGTTAAATCATTGAGTTCCACTGTGGCTGAGTTTATTAACACATTTAAGGTAACTCTAATCCCATAGCAGTTGATATAGTTTTATGTTTTAGTGTGCAAATGGCCACACCCTTTGTTCAAGCTGCAAGTCAAGGGTTCACAACTGCTGCCCTACGTGCCGCCATGAGCTTGGAAACATAAGGTGCCTAGTGCTGGAAAAGGTGGCCGAGTCGCTCGAACTCCCTTGTGTGTACCAAAATCTAGGCTGCATTGACACGCTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATATCGCCCCTACAACTGCCCTTATGCAGGAGGTGAATGTTCTGTAACTGGTGACATCCCGTACCTCGTCATGCATCTCAAGGACGATCACGAGGTTGATATGCATGAGGGATGTTCCTTCAGCCATAGATACGTCAAAGCCAATCCCCGAGAAGTTGAAAATGCTACATGGATGCTAACTGTGAGATCTGCCTCTCTGTTTCTCTCTGTTTATGGCTGGAATCTCCTGCATAAACTCTGATTTTCGTGTTTAGGATGAAAGAAGCAGCATTCCATTTAACATTCTCTGTTTTTCATATGAATCTATTGCAGGTATTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGGTTAGGAACAGCACCTGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGACGATGATGCGAAGAAATTTTCTTACAGTTTGGAAGTTGGTGGAAATGGGAGAAAACTCATGTGGCAAGGTACGCCGAGAAGCATCAGGGATAGCCATCGGAAGGTTCGGGACAGTCAGGATGGATTGATCATTCACAGGAACTTGGCTCTGTTCTGCTCTGGAAATGATGGCCAGGAACTGAAGCTCAAGGTTGTAGGACGAATATGGAGAGTACGG

mRNA sequence

CCCATGAGTATTGTAGAATTCAGAGAGAAGGAGATGTACCAGCAGAAGAAGTTAAGGGAAGAACATACTTCTTCTTCTGGGGCCAGCTTCTGCAAGGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAAGCAGCAACTTCTCGTCCAGAGTTCAAAAGCGTCTCTTCCAGGAAATCTTCCACTGGTTTTGGCAGGAATTGTAACAATGTACATGAGCTACTAGATTGCACTGTTTGCATGGATTTAATGCACCCTCCAATTTACCAGTGTGCAAATGGCCACACCCTTTGTTCAAGCTGCAAGTCAAGGGTTCACAACTGCTGCCCTACGTGCCGCCATGAGCTTGGAAACATAAGGTGCCTAGTGCTGGAAAAGGTGGCCGAGTCGCTCGAACTCCCTTGTGTGTACCAAAATCTAGGCTGCATTGACACGCTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATATCGCCCCTACAACTGCCCTTATGCAGGAGGTGAATGTTCTGTAACTGGTGACATCCCGTACCTCGTCATGCATCTCAAGGACGATCACGAGGTTGATATGCATGAGGGATGTTCCTTCAGCCATAGATACGTCAAAGCCAATCCCCGAGAAGTTGAAAATGCTACATGGATGCTAACTGTATTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGGTTAGGAACAGCACCTGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGACGATGATGCGAAGAAATTTTCTTACAGTTTGGAAGTTGGTGGAAATGGGAGAAAACTCATGTGGCAAGGTACGCCGAGAAGCATCAGGGATAGCCATCGGAAGGTTCGGGACAGTCAGGATGGATTGATCATTCACAGGAACTTGGCTCTGTTCTGCTCTGGAAATGATGGCCAGGAACTGAAGCTCAAGGTTGTAGGACGAATATGGAGAGTACGG

Coding sequence (CDS)

CCCATGAGTATTGTAGAATTCAGAGAGAAGGAGATGTACCAGCAGAAGAAGTTAAGGGAAGAACATACTTCTTCTTCTGGGGCCAGCTTCTGCAAGGAAGTGACCGAGTTGCGCCTTACATCGATAGATTCTCAAGCAGCAACTTCTCGTCCAGAGTTCAAAAGCGTCTCTTCCAGGAAATCTTCCACTGGTTTTGGCAGGAATTGTAACAATGTACATGAGCTACTAGATTGCACTGTTTGCATGGATTTAATGCACCCTCCAATTTACCAGTGTGCAAATGGCCACACCCTTTGTTCAAGCTGCAAGTCAAGGGTTCACAACTGCTGCCCTACGTGCCGCCATGAGCTTGGAAACATAAGGTGCCTAGTGCTGGAAAAGGTGGCCGAGTCGCTCGAACTCCCTTGTGTGTACCAAAATCTAGGCTGCATTGACACGCTCCCATACTACAGCAAGCTTAAACATGAGAAGAACTGTAAATATCGCCCCTACAACTGCCCTTATGCAGGAGGTGAATGTTCTGTAACTGGTGACATCCCGTACCTCGTCATGCATCTCAAGGACGATCACGAGGTTGATATGCATGAGGGATGTTCCTTCAGCCATAGATACGTCAAAGCCAATCCCCGAGAAGTTGAAAATGCTACATGGATGCTAACTGTATTTAACTGCTTTGGACGACAATTCTGTTTGCACTTTGAAGCATTTCGGTTAGGAACAGCACCTGTTTACATGGCGTTCCTCCGGTTCATGGGCGACGACGATGATGCGAAGAAATTTTCTTACAGTTTGGAAGTTGGTGGAAATGGGAGAAAACTCATGTGGCAAGGTACGCCGAGAAGCATCAGGGATAGCCATCGGAAGGTTCGGGACAGTCAGGATGGATTGATCATTCACAGGAACTTGGCTCTGTTCTGCTCTGGAAATGATGGCCAGGAACTGAAGCTCAAGGTTGTAGGACGAATATGGAGAGTACGG

Protein sequence

PMSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSSTGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWRVR

Homology

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BLAST of MS010241 vs. NCBI nr
Match: XP_022153850.1 (putative E3 ubiquitin-protein ligase SINAT1 [Momordica charantia] >XP_022153851.1 putative E3 ubiquitin-protein ligase SINAT1 [Momordica charantia])

HSP 1 Score: 688.7 bits (1776), Expect = 2.4e-194
Identity = 323/325 (99.38%), Postives = 324/325 (99.69%), Query Frame = 0


Query: 2   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 61
           MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS
Sbjct: 1   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 60

Query: 62  STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 121
           STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR
Sbjct: 61  STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 120

Query: 122 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 181
           CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY
Sbjct: 121 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 180

Query: 182 LVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 241
           LVMHLKDDHEVDMHEGCSFSHRYVK+NPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 181 LVMHLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 240

Query: 242 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 301
           PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN
Sbjct: 241 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 300

Query: 302 LALFCSGNDGQELKLKVVGRIWRVR 327
           LALFCSGN GQELKLKVVGRIWRVR
Sbjct: 301 LALFCSGNHGQELKLKVVGRIWRVR 325

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BLAST of MS010241 vs. NCBI nr
Match: XP_038900383.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 4.9e-171
Identity = 286/325 (88.00%), Postives = 303/325 (93.23%), Query Frame = 0


Query: 2   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 61
           M+ VEFREK MYQQ+K REEH + S ASFCKEVTELRLTSIDSQAATSRPEFK ++ RK+
Sbjct: 13  MTTVEFREKGMYQQQKSREEH-AFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKA 72

Query: 62  ST-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNI 121
           ST GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+R+ NCCPTCRHELGNI
Sbjct: 73  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 132

Query: 122 RCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 181
           RCL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 133 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 192

Query: 182 YLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGT 241
           YLVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 193 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 252

Query: 242 APVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHR 301
           APVYMAFLRFMGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R
Sbjct: 253 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 312

Query: 302 NLALFCSGNDGQELKLKVVGRIWRV 326
            LALFCSGN+G ELKLKV GRIWRV
Sbjct: 313 KLALFCSGNEGMELKLKVTGRIWRV 336

[Close]

BLAST of MS010241 vs. NCBI nr
Match: XP_038900384.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_038900386.1 E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 4.9e-171
Identity = 286/325 (88.00%), Postives = 303/325 (93.23%), Query Frame = 0


Query: 2   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 61
           M+ VEFREK MYQQ+K REEH + S ASFCKEVTELRLTSIDSQAATSRPEFK ++ RK+
Sbjct: 1   MTTVEFREKGMYQQQKSREEH-AFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKA 60

Query: 62  ST-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNI 121
           ST GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+R+ NCCPTCRHELGNI
Sbjct: 61  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 120

Query: 122 RCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 181
           RCL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 121 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 180

Query: 182 YLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGT 241
           YLVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 181 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 240

Query: 242 APVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHR 301
           APVYMAFLRFMGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R
Sbjct: 241 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 300

Query: 302 NLALFCSGNDGQELKLKVVGRIWRV 326
            LALFCSGN+G ELKLKV GRIWRV
Sbjct: 301 KLALFCSGNEGMELKLKVTGRIWRV 324

[Close]

BLAST of MS010241 vs. NCBI nr
Match: XP_038900382.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 611.3 bits (1575), Expect = 4.9e-171
Identity = 286/325 (88.00%), Postives = 303/325 (93.23%), Query Frame = 0


Query: 2   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 61
           M+ VEFREK MYQQ+K REEH + S ASFCKEVTELRLTSIDSQAATSRPEFK ++ RK+
Sbjct: 13  MTTVEFREKGMYQQQKSREEH-AFSAASFCKEVTELRLTSIDSQAATSRPEFKGITFRKA 72

Query: 62  ST-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNI 121
           ST GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+R+ NCCPTCRHELGNI
Sbjct: 73  STGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARIQNCCPTCRHELGNI 132

Query: 122 RCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIP 181
           RCL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP
Sbjct: 133 RCLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECRVTGDIP 192

Query: 182 YLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGT 241
           YLVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGT
Sbjct: 193 YLVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGT 252

Query: 242 APVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHR 301
           APVYMAFLRFMGDDDDAK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R
Sbjct: 253 APVYMAFLRFMGDDDDAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQR 312

Query: 302 NLALFCSGNDGQELKLKVVGRIWRV 326
            LALFCSGN+G ELKLKV GRIWRV
Sbjct: 313 KLALFCSGNEGMELKLKVTGRIWRV 336

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BLAST of MS010241 vs. NCBI nr
Match: XP_031740164.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetical protein Csa_018085 [Cucumis sativus])

HSP 1 Score: 605.9 bits (1561), Expect = 2.0e-169
Identity = 285/325 (87.69%), Postives = 301/325 (92.62%), Query Frame = 0


Query: 3   SIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSS 62
           +I+EFREK MYQQ K REEH +SS ASFCKEVTELRLTSIDSQ ATSRPEFK V+ RK+S
Sbjct: 3   TIIEFREKGMYQQPKSREEH-ASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKAS 62

Query: 63  T-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 122
           T GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNIR
Sbjct: 63  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 122

Query: 123 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 182
           CL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP 
Sbjct: 123 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPS 182

Query: 183 LVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242
           LVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 183 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242

Query: 243 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 302
           PVYMAFLRFMGDD +AK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R 
Sbjct: 243 PVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 302

Query: 303 LALFCSGNDGQELKLKVVGRIWRVR 327
           LALFCSGN+G ELKLKV GRIWRVR
Sbjct: 303 LALFCSGNEGMELKLKVTGRIWRVR 326

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BLAST of MS010241 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.9e-131
Identity = 218/303 (71.95%), Postives = 251/303 (82.84%), Query Frame = 0


Query: 27  GASFCKEVTELRLTSIDSQAATSRPEFKSVSSRK-SSTGFGR----NCNNVHELLDCTVC 86
           G S  KEV E   T +D +  T++ E  +    K  S G G+    + N V+ELL+C VC
Sbjct: 4   GGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVC 63

Query: 87  MDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNL 146
            +LM+PPI+QC NGHTLCS+CK RV N CPTCR+ELGNIRCL LEKVAESLE+PC YQNL
Sbjct: 64  TNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNL 123

Query: 147 GCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFS 206
           GC D  PYYSKLKHE++C++RPY CPYAG ECSVTGDIP LV+HLKDDH+VDMH+GC+F+
Sbjct: 124 GCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFN 183

Query: 207 HRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFS 266
           HRYVK+NP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AKKFS
Sbjct: 184 HRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFS 243

Query: 267 YSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGR 325
           YSLEVG +GRKL WQG PRSIRDSHRKVRDSQDGLII RNLAL+ SG D QELKL+V GR
Sbjct: 244 YSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGR 303

[Close]

BLAST of MS010241 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 1.6e-129
Identity = 213/300 (71.00%), Postives = 248/300 (82.67%), Query Frame = 0


Query: 27  GASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSSTGFGR--NCNNVHELLDCTVCMDL 86
           G S  KE  E   T +D +   ++ E  S  ++  S   G+  + N V+ELL+C VC +L
Sbjct: 4   GGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNL 63

Query: 87  MHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCI 146
           M+PPI+QC NGHTLCSSCK RV N CPTCR+ELGNIRCL LEKVAESLE+PC YQNLGC 
Sbjct: 64  MYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQ 123

Query: 147 DTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRY 206
           D  PYYSKLKHE++C++R Y+CPYAG ECSVTGDIP LV HLKDDH+VDMH+GC+F+HRY
Sbjct: 124 DIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRY 183

Query: 207 VKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSL 266
           VK+NP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AKKFSYSL
Sbjct: 184 VKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSL 243

Query: 267 EVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWR 325
           EVG + RKL WQG PRSIRDSHRKVRDSQDGLII RNLAL+ SG+D +ELKL+V GRIW+
Sbjct: 244 EVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of MS010241 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 3.0e-123
Identity = 193/255 (75.69%), Postives = 221/255 (86.67%), Query Frame = 0


Query: 70  NNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVA 129
           ++V ELL+C VC+  M+PPI+QC+NGHTLCS CK RVHN CPTCRHELGNIRCL LEKVA
Sbjct: 45  SSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKVA 104

Query: 130 ESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDD 189
            SLELPC YQN GC+   PYY KLKHE  C+YRPY+CPYAG EC+V GDIPYLV HLKDD
Sbjct: 105 ASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKDD 164

Query: 190 HEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLR 249
           H+VDMH GC+F+HRYVK+NP EVENATWMLTVF+CFG+ FCLHFEAF+LG APVY+AFLR
Sbjct: 165 HKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFLR 224

Query: 250 FMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGN 309
           FMGDD +AK +SYSLEVGG GRK++WQG PRSIRDSHRKVRDS DGLII RN+ALF SG 
Sbjct: 225 FMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSGG 284

Query: 310 DGQELKLKVVGRIWR 325
           + +ELKL+V GRIW+
Sbjct: 285 ERKELKLRVTGRIWK 299

[Close]

BLAST of MS010241 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 441.8 bits (1135), Expect = 6.7e-123
Identity = 196/283 (69.26%), Postives = 231/283 (81.63%), Query Frame = 0


Query: 42  IDSQAATSRPEFKSVSSRKSSTGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSS 101
           ID      + +F S  S+  +T       +V+ELL+C VC + M+PPI+QC NGHTLCS+
Sbjct: 10  IDDDEIHQKHQFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNGHTLCST 69

Query: 102 CKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKY 161
           CKSRVHN CPTCR ELG+IRCL LEKVAESLELPC Y NLGC+   PYYSKLKHE  C +
Sbjct: 70  CKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNF 129

Query: 162 RPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTV 221
           RPY+CPYAG EC+  GDI +LV HL+DDH+VDMH GC+F+HRYVK+NPREVENATWMLTV
Sbjct: 130 RPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTV 189

Query: 222 FNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRS 281
           F CFG+ FCLHFEAF+LG APVYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+GTPRS
Sbjct: 190 FQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRS 249

Query: 282 IRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWR 325
           +RDSHRKVRDS DGLII RN+ALF SG D +ELKL+V GRIW+
Sbjct: 250 VRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWK 292

[Close]

BLAST of MS010241 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 9.6e-122
Identity = 198/293 (67.58%), Postives = 235/293 (80.20%), Query Frame = 0


Query: 43  DSQAATSRPEFKSVSSRKSSTGFGRNCN-----------NVHELLDCTVCMDLMHPPIYQ 102
           D +    R  + SVS    +     N N           +VHELL+C VC + M+PPI+Q
Sbjct: 17  DEEIHQDRHSYASVSKHHHTNNNTTNVNAAASGLLPTTTSVHELLECPVCTNSMYPPIHQ 76

Query: 103 CANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCIDTLPYYS 162
           C NGHTLCS+CK+RVHN CPTCR ELG+IRCL LEKVAESLELPC + +LGC +  PYYS
Sbjct: 77  CHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYS 136

Query: 163 KLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRYVKANPRE 222
           KLKHE  C +RPY+CPYAG ECSVTGDIP+LV HL+DDH+VDMH GC+F+HRYVK+NPRE
Sbjct: 137 KLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPRE 196

Query: 223 VENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGR 282
           VENATWMLTVF+CFG+ FCLHFEAF+LG APVYMAFLRFMGD+ +A+ ++YSLEVGG GR
Sbjct: 197 VENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGR 256

Query: 283 KLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWR 325
           KL+W+GTPRS+RDSHRKVRDS DGLII RN+ALF SG D +ELKL+V GRIW+
Sbjct: 257 KLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of MS010241 vs. ExPASy TrEMBL
Match: A0A6J1DI06 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111021266 PE=3 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 1.2e-194
Identity = 323/325 (99.38%), Postives = 324/325 (99.69%), Query Frame = 0


Query: 2   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 61
           MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS
Sbjct: 1   MSIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKS 60

Query: 62  STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 121
           STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR
Sbjct: 61  STGFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 120

Query: 122 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 181
           CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY
Sbjct: 121 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 180

Query: 182 LVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 241
           LVMHLKDDHEVDMHEGCSFSHRYVK+NPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 181 LVMHLKDDHEVDMHEGCSFSHRYVKSNPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 240

Query: 242 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 301
           PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN
Sbjct: 241 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 300

Query: 302 LALFCSGNDGQELKLKVVGRIWRVR 327
           LALFCSGN GQELKLKVVGRIWRVR
Sbjct: 301 LALFCSGNHGQELKLKVVGRIWRVR 325

[Close]

BLAST of MS010241 vs. ExPASy TrEMBL
Match: A0A0A0KWV9 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 9.9e-170
Identity = 285/325 (87.69%), Postives = 301/325 (92.62%), Query Frame = 0


Query: 3   SIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSS 62
           +I+EFREK MYQQ K REEH +SS ASFCKEVTELRLTSIDSQ ATSRPEFK V+ RK+S
Sbjct: 3   TIIEFREKGMYQQPKSREEH-ASSAASFCKEVTELRLTSIDSQPATSRPEFKGVTFRKAS 62

Query: 63  T-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 122
           T GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNIR
Sbjct: 63  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 122

Query: 123 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 182
           CL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP 
Sbjct: 123 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPS 182

Query: 183 LVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242
           LVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 183 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242

Query: 243 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 302
           PVYMAFLRFMGDD +AK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R 
Sbjct: 243 PVYMAFLRFMGDDSEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 302

Query: 303 LALFCSGNDGQELKLKVVGRIWRVR 327
           LALFCSGN+G ELKLKV GRIWRVR
Sbjct: 303 LALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of MS010241 vs. ExPASy TrEMBL
Match: A0A1S3BMT4 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.7e-169
Identity = 284/325 (87.38%), Postives = 302/325 (92.92%), Query Frame = 0


Query: 3   SIVEFREKEMYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSS 62
           +I+EFREK MYQQ+K REEH +SS ASFCKEVTEL LTSIDSQ ATSRPEFK V+ RK+S
Sbjct: 3   TIIEFREKGMYQQQKSREEH-ASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKAS 62

Query: 63  T-GFGRNCNNVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIR 122
           T GF R+ N+VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNIR
Sbjct: 63  TGGFARSRNDVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIR 122

Query: 123 CLVLEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPY 182
           CL LEKVAESLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP 
Sbjct: 123 CLALEKVAESLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPS 182

Query: 183 LVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242
           LVMHLKDDHEVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTA
Sbjct: 183 LVMHLKDDHEVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTA 242

Query: 243 PVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRN 302
           PVYMAFLRFMGDD++AK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R 
Sbjct: 243 PVYMAFLRFMGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRK 302

Query: 303 LALFCSGNDGQELKLKVVGRIWRVR 327
           LALFCSGN+G ELKLKV GRIWRVR
Sbjct: 303 LALFCSGNEGMELKLKVTGRIWRVR 326

[Close]

BLAST of MS010241 vs. ExPASy TrEMBL
Match: A0A5D3DDZ5 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00330 PE=3 SV=1)

HSP 1 Score: 593.6 bits (1529), Expect = 5.1e-166
Identity = 278/316 (87.97%), Postives = 294/316 (93.04%), Query Frame = 0


Query: 12  MYQQKKLREEHTSSSGASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSST-GFGRNCN 71
           MYQQ+K REEH +SS ASFCKEVTEL LTSIDSQ ATSRPEFK V+ RK+ST GF R+ N
Sbjct: 1   MYQQQKSREEH-ASSAASFCKEVTELHLTSIDSQPATSRPEFKGVTFRKASTGGFARSRN 60

Query: 72  NVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAE 131
           +VHELLDCTVCM+LMHPPIYQCANGHTLCSSCK+RV NCCPTCRHELGNIRCL LEKVAE
Sbjct: 61  DVHELLDCTVCMNLMHPPIYQCANGHTLCSSCKARVQNCCPTCRHELGNIRCLALEKVAE 120

Query: 132 SLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDH 191
           SLELPCVYQNLGCID  PYYSKLKHEKNCKYRPYNCPYAGGEC VTGDIP LVMHLKDDH
Sbjct: 121 SLELPCVYQNLGCIDMFPYYSKLKHEKNCKYRPYNCPYAGGECHVTGDIPSLVMHLKDDH 180

Query: 192 EVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 251
           EVDMH+GCSFSHRYVK+NP+EVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF
Sbjct: 181 EVDMHDGCSFSHRYVKSNPQEVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 240

Query: 252 MGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGND 311
           MGDD++AK+FSYSLEVGGNGRKL+WQG PRSIRDSHRKVRDS DGLII R LALFCSGN+
Sbjct: 241 MGDDNEAKQFSYSLEVGGNGRKLIWQGIPRSIRDSHRKVRDSHDGLIIQRKLALFCSGNE 300

Query: 312 GQELKLKVVGRIWRVR 327
           G ELKLKV GRIWRVR
Sbjct: 301 GMELKLKVTGRIWRVR 315

[Close]

BLAST of MS010241 vs. ExPASy TrEMBL
Match: A0A1R3GPA5 (RING-type E3 ubiquitin transferase OS=Corchorus olitorius OX=93759 GN=COLO4_34000 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.8e-138
Identity = 228/303 (75.25%), Postives = 256/303 (84.49%), Query Frame = 0


Query: 27  GASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSSTGFG-----RNCNNVHELLDCTVC 86
           G   CK+V E R+   D   ATS  E +    RK++TG G      + N+VHELL+C VC
Sbjct: 4   GGGICKDVIESRIAFADYDMATSSAELRGSPLRKAATGLGGTFGAASTNDVHELLECPVC 63

Query: 87  MDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNL 146
           ++LM+PPIYQC NGHT+CS CK+RVHN CPTCR+ELGNIRCL LEKVAESLELPC YQ L
Sbjct: 64  INLMYPPIYQCPNGHTVCSICKARVHNSCPTCRNELGNIRCLALEKVAESLELPCKYQIL 123

Query: 147 GCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFS 206
           GC D  PYYSKLKHEKNC+YRPYNCPYAG ECSVTGDIP+LVMHLK+DH+VDMH+GC+F+
Sbjct: 124 GCHDIFPYYSKLKHEKNCRYRPYNCPYAGAECSVTGDIPFLVMHLKNDHKVDMHDGCTFN 183

Query: 207 HRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFS 266
           HRYVK+NP EVENATWMLTVFNCFGRQFCLHFEAF +G APVYMAFLRFMGD+D+A++FS
Sbjct: 184 HRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFHIGMAPVYMAFLRFMGDEDEARQFS 243

Query: 267 YSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGR 325
           YSLEVGGNGRK MWQG PRSIRDSHRKVRDSQDGLII RNLALF SG D QELKLKV GR
Sbjct: 244 YSLEVGGNGRKFMWQGVPRSIRDSHRKVRDSQDGLIIQRNLALFFSGGDRQELKLKVAGR 303

[Close]

BLAST of MS010241 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 469.2 bits (1206), Expect = 2.8e-132
Identity = 218/303 (71.95%), Postives = 251/303 (82.84%), Query Frame = 0


Query: 27  GASFCKEVTELRLTSIDSQAATSRPEFKSVSSRK-SSTGFGR----NCNNVHELLDCTVC 86
           G S  KEV E   T +D +  T++ E  +    K  S G G+    + N V+ELL+C VC
Sbjct: 4   GGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVC 63

Query: 87  MDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNL 146
            +LM+PPI+QC NGHTLCS+CK RV N CPTCR+ELGNIRCL LEKVAESLE+PC YQNL
Sbjct: 64  TNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNL 123

Query: 147 GCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFS 206
           GC D  PYYSKLKHE++C++RPY CPYAG ECSVTGDIP LV+HLKDDH+VDMH+GC+F+
Sbjct: 124 GCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFN 183

Query: 207 HRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFS 266
           HRYVK+NP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AKKFS
Sbjct: 184 HRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFS 243

Query: 267 YSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGR 325
           YSLEVG +GRKL WQG PRSIRDSHRKVRDSQDGLII RNLAL+ SG D QELKL+V GR
Sbjct: 244 YSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGR 303

[Close]

BLAST of MS010241 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-130
Identity = 213/300 (71.00%), Postives = 248/300 (82.67%), Query Frame = 0


Query: 27  GASFCKEVTELRLTSIDSQAATSRPEFKSVSSRKSSTGFGR--NCNNVHELLDCTVCMDL 86
           G S  KE  E   T +D +   ++ E  S  ++  S   G+  + N V+ELL+C VC +L
Sbjct: 4   GGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKFHSSNGVYELLECPVCTNL 63

Query: 87  MHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCI 146
           M+PPI+QC NGHTLCSSCK RV N CPTCR+ELGNIRCL LEKVAESLE+PC YQNLGC 
Sbjct: 64  MYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCQ 123

Query: 147 DTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRY 206
           D  PYYSKLKHE++C++R Y+CPYAG ECSVTGDIP LV HLKDDH+VDMH+GC+F+HRY
Sbjct: 124 DIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCTFNHRY 183

Query: 207 VKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSL 266
           VK+NP EVENATWMLTVFNCFGRQFCLHFEAF+LG APVYMAFLRFMGD+++AKKFSYSL
Sbjct: 184 VKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSL 243

Query: 267 EVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWR 325
           EVG + RKL WQG PRSIRDSHRKVRDSQDGLII RNLAL+ SG+D +ELKL+V GRIW+
Sbjct: 244 EVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVTGRIWK 303

[Close]

BLAST of MS010241 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 438.0 bits (1125), Expect = 6.8e-123
Identity = 198/293 (67.58%), Postives = 235/293 (80.20%), Query Frame = 0


Query: 43  DSQAATSRPEFKSVSSRKSSTGFGRNCN-----------NVHELLDCTVCMDLMHPPIYQ 102
           D +    R  + SVS    +     N N           +VHELL+C VC + M+PPI+Q
Sbjct: 17  DEEIHQDRHSYASVSKHHHTNNNTTNVNAAASGLLPTTTSVHELLECPVCTNSMYPPIHQ 76

Query: 103 CANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAESLELPCVYQNLGCIDTLPYYS 162
           C NGHTLCS+CK+RVHN CPTCR ELG+IRCL LEKVAESLELPC + +LGC +  PYYS
Sbjct: 77  CHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYS 136

Query: 163 KLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDHEVDMHEGCSFSHRYVKANPRE 222
           KLKHE  C +RPY+CPYAG ECSVTGDIP+LV HL+DDH+VDMH GC+F+HRYVK+NPRE
Sbjct: 137 KLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPRE 196

Query: 223 VENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGR 282
           VENATWMLTVF+CFG+ FCLHFEAF+LG APVYMAFLRFMGD+ +A+ ++YSLEVGG GR
Sbjct: 197 VENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGR 256

Query: 283 KLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGNDGQELKLKVVGRIWR 325
           KL+W+GTPRS+RDSHRKVRDS DGLII RN+ALF SG D +ELKL+V GRIW+
Sbjct: 257 KLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWK 309

[Close]

BLAST of MS010241 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 430.3 bits (1105), Expect = 1.4e-120
Identity = 188/254 (74.02%), Postives = 220/254 (86.61%), Query Frame = 0


Query: 71  NVHELLDCTVCMDLMHPPIYQCANGHTLCSSCKSRVHNCCPTCRHELGNIRCLVLEKVAE 130
           +V+ELL+C VC   M+PPI+QC NGHTLCS+CK RVHN CPTCR ELG+IRCL LEKVAE
Sbjct: 57  SVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAE 116

Query: 131 SLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTGDIPYLVMHLKDDH 190
           SLELPC + NLGC +  PYYSKLKHE  C +RPY+CPYAG EC + GDIP+LV HL+DDH
Sbjct: 117 SLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDH 176

Query: 191 EVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFRLGTAPVYMAFLRF 250
           +VDMH G +F+HRYVK+NPREVENATWMLTVF+CFG+ FCLHFEAF+LG  PVYMAFLRF
Sbjct: 177 KVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRF 236

Query: 251 MGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLIIHRNLALFCSGND 310
           MGD++DA+ +SYSLEVGG+GRKL W+GTPRSIRDSHRKVRDS DGLII RN+ALF SG D
Sbjct: 237 MGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGD 296

Query: 311 GQELKLKVVGRIWR 325
            +ELKL+V G+IW+
Sbjct: 297 RKELKLRVTGKIWK 310

[Close]

BLAST of MS010241 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.2e-96
Identity = 153/207 (73.91%), Postives = 178/207 (85.99%), Query Frame = 0


Query: 119 NIRCLV-LEKVAESLELPCVYQNLGCIDTLPYYSKLKHEKNCKYRPYNCPYAGGECSVTG 178
           N  C++ LEKVAESLELPC Y NLGC+   PYYSKLKHE  C +RPY+CPYAG EC+  G
Sbjct: 10  NQECIIALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVG 69

Query: 179 DIPYLVMHLKDDHEVDMHEGCSFSHRYVKANPREVENATWMLTVFNCFGRQFCLHFEAFR 238
           DI +LV HL+DDH+VDMH GC+F+HRYVK+NPREVENATWMLTVF CFG+ FCLHFEAF+
Sbjct: 70  DITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQ 129

Query: 239 LGTAPVYMAFLRFMGDDDDAKKFSYSLEVGGNGRKLMWQGTPRSIRDSHRKVRDSQDGLI 298
           LG APVYMAFLRFMGD+DDA+ ++YSLEVGG+GRK  W+GTPRS+RDSHRKVRDS DGLI
Sbjct: 130 LGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLI 189

Query: 299 IHRNLALFCSGNDGQELKLKVVGRIWR 325
           I RN+ALF SG D +ELKL+V GRIW+
Sbjct: 190 IQRNMALFFSGGDKKELKLRVTGRIWK 216

The following BLAST results are available for this feature:

BLAST of MS010241 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022153850.1	2.4e-194	99.38	putative E3 ubiquitin-protein ligase SINAT1 [Momordica charantia] >XP_022153851....	[more]
XP_038900383.1	4.9e-171	88.00	E3 ubiquitin-protein ligase SINAT2-like isoform X2 [Benincasa hispida]	[more]
XP_038900384.1	4.9e-171	88.00	E3 ubiquitin-protein ligase SINAT2-like isoform X3 [Benincasa hispida] >XP_03890...	[more]
XP_038900382.1	4.9e-171	88.00	E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Benincasa hispida]	[more]
XP_031740164.1	2.0e-169	87.69	E3 ubiquitin-protein ligase SINAT2-like [Cucumis sativus] >KGN54095.1 hypothetic...	[more]

BLAST of MS010241 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2P4	3.9e-131	71.95	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]
P93748	1.6e-129	71.00	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q10L91	3.0e-123	75.69	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
Q8S3N1	6.7e-123	69.26	E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=...	[more]
Q84JL3	9.6e-122	67.58	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]

BLAST of MS010241 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DI06	1.2e-194	99.38	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102126...	[more]
A0A0A0KWV9	9.9e-170	87.69	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_4G285710 PE...	[more]
A0A1S3BMT4	1.7e-169	87.38	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491565 PE=3 ...	[more]
A0A5D3DDZ5	5.1e-166	87.97	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1R3GPA5	5.8e-138	75.25	RING-type E3 ubiquitin transferase OS=Corchorus olitorius OX=93759 GN=COLO4_3400...	[more]

BLAST of MS010241 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58040.1	2.8e-132	71.95	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	1.2e-130	71.00	Protein with RING/U-box and TRAF-like domains	[more]
AT3G61790.1	6.8e-123	67.58	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	1.4e-120	74.02	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	3.2e-96	73.91	TRAF-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008974	TRAF-like	GENE3D	2.60.210.10	Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A	coord: 189..319 e-value: 1.4E-36 score: 127.2
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 32..130 e-value: 1.2E-5 score: 26.8
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 131..186 e-value: 1.8E-16 score: 62.1
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 120..318 e-value: 4.6E-79 score: 265.1
None	No IPR available	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 26..324
None	No IPR available	PANTHER	PTHR10315:SF123	E3 UBIQUITIN-PROTEIN LIGASE SINAT2-LIKE	coord: 26..324
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 76..114 e-value: 1.00199E-13 score: 62.3022
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 65..118
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 131..322
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 131..191 score: 13.869804
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 78..114 score: 9.346889

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010241.1	MS010241.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005515	protein binding
molecular_function	GO:0008270	zinc ion binding