MS010231 (gene) Bitter gourd (TR) v1

BLAST of MS010231 vs. NCBI nr
Match: KAG6570371.1 (hypothetical protein SDJN03_29286, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 4169.4 bits (10812), Expect = 0.0e+00
Identity = 2078/2322 (89.49%), Postives = 2192/2322 (94.40%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            MDTGK LEDQFSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGY++VTVLEKHQ VGGM
Sbjct: 2    MDTGKPLEDQFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYSDVTVLEKHQDVGGM 61

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLA+ETGSELEEMDSHKLALI T+G+YQDIGVA
Sbjct: 62   CESVEIEGKIYDLGGQVLAANSAPTIFHLAQETGSELEEMDSHKLALIHTSGEYQDIGVA 121

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TLELQDKAKDSGHIGVHAVS FASDLTPAYLEAHGLTSVPKSVAYGYTASG
Sbjct: 122  DDYTSIISLTLELQDKAKDSGHIGVHAVSAFASDLTPAYLEAHGLTSVPKSVAYGYTASG 181

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGFLQDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISES+PIKVHCNTEVVSVRRSF+
Sbjct: 182  YGFLQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESIPIKVHCNTEVVSVRRSFK 241

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            +V LHVMD D NLTS EFDK+IISGSFPF++GRTYRSSTTK SEEG E MDMSHLEKELF
Sbjct: 242  TVTLHVMDLDTNLTSWEFDKIIISGSFPFRNGRTYRSSTTKSSEEGAETMDMSHLEKELF 301

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKIEGL+HLPLGFYYFG+HMDNPETIGYPVAMQRFY DTDIFLFWSYG
Sbjct: 302  SKVYTIDYYTTVLKIEGLHHLPLGFYYFGEHMDNPETIGYPVAMQRFYADTDIFLFWSYG 361

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGP V ELAINTVK MG EVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 362  NSADITGPKVAELAINTVKKMGAEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 421

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYYVGGLMAFELTERNSSYAM+LVCKHFA NNSSPMFSYAKPMF LQSKRE+ +KGL 
Sbjct: 422  LNTYYVGGLMAFELTERNSSYAMTLVCKHFA-NNSSPMFSYAKPMFFLQSKRERDVKGLG 481

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFPDL+SLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 482  ELPGVEFPDLNSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 541

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKPPIKPGDR+LLIYVPGLDFIDAFFGCLRAK++PVPVLPPDPLQRGGQALLKIEY
Sbjct: 542  LLSNQKPPIKPGDRVLLIYVPGLDFIDAFFGCLRAKILPVPVLPPDPLQRGGQALLKIEY 601

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSCGAVAILSTL YHSAVRVGKVKNMI  +RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 602  IAKSCGAVAILSTLSYHSAVRVGKVKNMIGLMRENGKSSAVWPKLPWMHTDSWIKNFANL 661

Query: 661  APEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS 720
            AP+ + NQSEPH DDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS
Sbjct: 662  APDAMTNQSEPHSDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS 721

Query: 721  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFEL 780
            WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIK PLLWL  +STY+ATHSAGPNFAFEL
Sbjct: 722  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKKPLLWLHVMSTYKATHSAGPNFAFEL 781

Query: 781  VARRLEA-NKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            VARRLEA NK K Q Y+LSS+VFLM+AAEP+RK TLK+F+ELT P GL+EEVMAPGYGLA
Sbjct: 782  VARRLEANNKGKAQTYDLSSMVFLMIAAEPIRKTTLKKFLELTSPFGLTEEVMAPGYGLA 841

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG PI+ID QGRVCCGYV+Q NADIDIR VNPGTG ELEEDGKEGEIWI
Sbjct: 842  ENCVFVSCAFGEGIPIYIDWQGRVCCGYVNQGNADIDIRIVNPGTGTELEEDGKEGEIWI 901

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQDTFRNELQN  GRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 902  SSPSAGIGYWGREELSQDTFRNELQNHHGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 961

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGIP+PDCSDQVG+VVIAEVKDGK
Sbjct: 962  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIPVPDCSDQVGLVVIAEVKDGK 1021

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            PVAKD+IDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1022 PVAKDIIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1081

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA----SVQPSPRISNKDIEEFLKGLVSE 1140
            AIKLRR FLRSFSTGTCKEGNTPRPQL+NLSRA    SVQP PRISNKDIEEFLKGLVSE
Sbjct: 1082 AIKLRRNFLRSFSTGTCKEGNTPRPQLTNLSRAFVQPSVQPGPRISNKDIEEFLKGLVSE 1141

Query: 1141 LTNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISEN 1200
            LTNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTA+CIA+LA ISEN
Sbjct: 1142 LTNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTATCIADLARISEN 1201

Query: 1201 ILNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFS 1260
            IL KNHAQSTKN ANPTFET CALIEMEKIS TR+F IWFF LLALI VA+ML FPAY S
Sbjct: 1202 ILTKNHAQSTKNTANPTFETTCALIEMEKISWTRRFGIWFFQLLALILVAMMLAFPAYLS 1261

Query: 1261 ISAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT 1320
            ISAF SS+ ILH  TD IPLMNYLLPLTLAPLAWILCIVSSC+CI+F GNSFLRPNYALT
Sbjct: 1262 ISAFISSMPILHTFTDHIPLMNYLLPLTLAPLAWILCIVSSCMCISFLGNSFLRPNYALT 1321

Query: 1321 PEVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDIT 1380
            PEVS+WS+DFVKWWA YKAQ+VSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDTI IT
Sbjct: 1322 PEVSLWSMDFVKWWAFYKAQEVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTIGIT 1381

Query: 1381 DPSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEV 1440
            DPSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGA LGEE EV
Sbjct: 1382 DPSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGAILGEEVEV 1441

Query: 1441 PALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYF 1500
            PALQKIEGIV TS   NL+K SKPQ  AG+RQEL AIYHFLGIYL+GFLGSLSAAIVYYF
Sbjct: 1442 PALQKIEGIVTTSVIGNLEKRSKPQRTAGERQELVAIYHFLGIYLLGFLGSLSAAIVYYF 1501

Query: 1501 YIWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGI 1560
            YIWLSQSSPSLQHLAFI L G FHW PFT IAYATIFAEVPSNATSFA+LFSS YLFHGI
Sbjct: 1502 YIWLSQSSPSLQHLAFICLVGAFHWMPFTVIAYATIFAEVPSNATSFAVLFSSMYLFHGI 1561

Query: 1561 ILCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAV 1620
            I CILTF++K LLT K  MEQNPLKIWLCHRI  ASHLRFA LLSGTEAFCIYLRLLGAV
Sbjct: 1562 IFCILTFVMKSLLTNKSKMEQNPLKIWLCHRIITASHLRFANLLSGTEAFCIYLRLLGAV 1621

Query: 1621 VGKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQS 1680
            +GKHCSIRAINPV DPELI IRT VHLGDFSRIISGFYST GL+RGKIE+Q+N+VIGSQS
Sbjct: 1622 IGKHCSIRAINPVLDPELIYIRTGVHLGDFSRIISGFYSTGGLSRGKIEIQDNSVIGSQS 1681

Query: 1681 TVLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYK 1740
             VL GSV+QE+VILGALSVAPMNSTLIKGGVYVGSRTPVMIKNT H+LDERIEKMD  YK
Sbjct: 1682 IVLLGSVIQEDVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTKHMLDERIEKMDTEYK 1741

Query: 1741 KIVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRH 1800
            KIVGNL+ANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGK+YPVFIRH
Sbjct: 1742 KIVGNLSANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKSYPVFIRH 1801

Query: 1801 SNSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAA 1860
            SNSLSADDDAR+DARGAALRI SD SD++PLLDLTLKTGNAFYARTIADFATWLVCGLAA
Sbjct: 1802 SNSLSADDDARVDARGAALRILSDGSDSTPLLDLTLKTGNAFYARTIADFATWLVCGLAA 1861

Query: 1861 REEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTIN 1920
            REEHVK+VPHIR+AVWNSLRL+DSY++LHYYSNICRLFRFKDGQEMYVK KLRPYD+TIN
Sbjct: 1862 REEHVKKVPHIRNAVWNSLRLADSYSKLHYYSNICRLFRFKDGQEMYVKLKLRPYDRTIN 1921

Query: 1921 EDSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHG 1980
            EDSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF SRVNSPGG+RYVFQLQ+RPV   
Sbjct: 1922 EDSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLSRVNSPGGVRYVFQLQMRPVPQD 1981

Query: 1981 EVDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSA 2040
            E D+DIALDCTKPWDE EFP +DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS 
Sbjct: 1982 EADQDIALDCTKPWDETEFPLIDIGEIEIHQSLSKEESEALEFNPFLRCDEVDVISATSV 2041

Query: 2041 SQSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKA 2100
            SQSASIDHGRSLIYEICQHLRNGSPLPEAWKIFL+QSDTKVDLSGCPVAAA+K R NEKA
Sbjct: 2042 SQSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLQQSDTKVDLSGCPVAAALKERGNEKA 2101

Query: 2101 ALNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVS 2160
            AL+RTW+QNFW+TF QPLLQTALPY+IIGL  FFPLA V+HLKEDKKLPL WLLPL+WVS
Sbjct: 2102 ALDRTWYQNFWLTFCQPLLQTALPYYIIGLATFFPLACVVHLKEDKKLPLHWLLPLMWVS 2161

Query: 2161 SGIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIF 2220
            SGIIAALCCV+AKWVLVQ+KK+GESIGIWS+RIFMDTIWQ I+T+VGDYFMEMT+GSFIF
Sbjct: 2162 SGIIAALCCVVAKWVLVQRKKEGESIGIWSVRIFMDTIWQGIKTVVGDYFMEMTSGSFIF 2221

Query: 2221 VLIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGK 2280
            V+IMKLMGSDVDL+QG+YVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG 
Sbjct: 2222 VVIMKLMGSDVDLEQGSYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGN 2281

Query: 2281 IEIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            IEIGEGG VGSRAIAMPGVRVESEA+LA LSLAMKEEI+RAS
Sbjct: 2282 IEIGEGGFVGSRAIAMPGVRVESEATLAPLSLAMKEEIIRAS 2322

BLAST of MS010231 vs. NCBI nr
Match: KAG7010248.1 (fadD28, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 4166.7 bits (10805), Expect = 0.0e+00
Identity = 2076/2321 (89.44%), Postives = 2190/2321 (94.36%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            MDTGK LEDQFSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGY++VTVLEKHQ VGGM
Sbjct: 1    MDTGKPLEDQFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYSDVTVLEKHQDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLA+ETGSELEEMDSHKLALI T+G+YQDIGVA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAQETGSELEEMDSHKLALIHTSGEYQDIGVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TLELQDKAKDSGHIGVHAVS FASDLTPAYLEAHGLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLELQDKAKDSGHIGVHAVSAFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGFLQDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISES+PIKVHCNTEVVSVRRSF+
Sbjct: 181  YGFLQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESIPIKVHCNTEVVSVRRSFK 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            +V LHVMD D NLTS EFDK+IISGSFPF++GRTYRSSTTK SEEG E MDMSHLEKELF
Sbjct: 241  TVTLHVMDLDTNLTSWEFDKIIISGSFPFRNGRTYRSSTTKSSEEGAETMDMSHLEKELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKIEGL+HLPLGFYYFG+HMDNPETIGYPVAMQRFY DTDIFLFWSYG
Sbjct: 301  SKVYTIDYYTTVLKIEGLHHLPLGFYYFGEHMDNPETIGYPVAMQRFYADTDIFLFWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGP V ELAINTVK MG EVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPKVAELAINTVKKMGAEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYYVGGLMAFELTERNSSYAM+LVCKHFA NNSSPMFSYAKPMF LQSKRE+ +KGL 
Sbjct: 421  LNTYYVGGLMAFELTERNSSYAMTLVCKHFA-NNSSPMFSYAKPMFFLQSKRERDVKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFPDL+SLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPDLNSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKPPIKPGDR+LLIYVPGLDFIDAFFGCLRAK++PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPPIKPGDRVLLIYVPGLDFIDAFFGCLRAKILPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSCGAVAILSTL YHSAVRVGKVKNMI  +RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCGAVAILSTLSYHSAVRVGKVKNMIGLMRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  APEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS 720
            AP+ + NQSEPH DDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS
Sbjct: 661  APDAMTNQSEPHSDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS 720

Query: 721  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFEL 780
            WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIK PLLWL  +STY+ATHSAGPNFAFEL
Sbjct: 721  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKKPLLWLHVMSTYKATHSAGPNFAFEL 780

Query: 781  VARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAE 840
            VARRLEANK K Q Y+LSS+VFLM+AAEP+RK TLK+F+ELT P GL+EEVMAPGYGLAE
Sbjct: 781  VARRLEANKGKAQTYDLSSMVFLMIAAEPIRKTTLKKFLELTSPFGLTEEVMAPGYGLAE 840

Query: 841  NCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWIS 900
            NCVFVSCAFGEG PIFID QGRVCCGYVDQ NADIDIR VNPGTG ELEEDGKEGEIWIS
Sbjct: 841  NCVFVSCAFGEGIPIFIDWQGRVCCGYVDQGNADIDIRIVNPGTGTELEEDGKEGEIWIS 900

Query: 901  SPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAGR 960
            SPSAGIGYWGREELSQDTFRNELQN  GRRY RTGDLGR+IDGKLFITGRIKDLIIAAGR
Sbjct: 901  SPSAGIGYWGREELSQDTFRNELQNHHGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAGR 960

Query: 961  NIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGKP 1020
            NIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGIP+PDCSDQVG+VVIAEVKDGKP
Sbjct: 961  NIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIPVPDCSDQVGLVVIAEVKDGKP 1020

Query: 1021 VAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPEA 1080
            VAKD+IDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPEA
Sbjct: 1021 VAKDIIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPEA 1080

Query: 1081 IKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA----SVQPSPRISNKDIEEFLKGLVSEL 1140
            IKLRR FLRSFSTGTCKEGNTPRPQL+NLSRA    SVQP PRISNKDIEEFLKGLVSEL
Sbjct: 1081 IKLRRNFLRSFSTGTCKEGNTPRPQLTNLSRAFVRPSVQPGPRISNKDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTA+CIA+LASISENI
Sbjct: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTATCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L KNHAQSTKN AN T ET CALIEMEKIS TR+F IWFF LLALI VA+ML FPAY SI
Sbjct: 1201 LAKNHAQSTKNTANSTCETTCALIEMEKISWTRRFGIWFFQLLALILVAMMLAFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAF SS+ ILH  TD IPLMNYLLPLTLAPLAWILCIVSSC+CI+F GNSFLRPNYALTP
Sbjct: 1261 SAFISSMPILHTFTDHIPLMNYLLPLTLAPLAWILCIVSSCMCISFLGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA YKAQ+VSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDTI ITD
Sbjct: 1321 EVSIWSMDFVKWWAFYKAQEVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTIGITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKG  LGEE EVP
Sbjct: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGGILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQKIEGIV TS   NL+K SKPQ  AG+RQEL AIYHFLGIYL+GFLGSLSAAIVYYFY
Sbjct: 1441 ALQKIEGIVTTSVIGNLEKRSKPQRTAGERQELVAIYHFLGIYLLGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQHLAFI L G FHW PFT IAYATIFAEVPSNATSFA+LFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHLAFICLVGAFHWMPFTVIAYATIFAEVPSNATSFAVLFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
             CILTF++K LLT K  MEQNPLK+WLCHRI  ASHLRFA LLSGTEAFCIYLRLLGAV+
Sbjct: 1561 FCILTFVMKSLLTNKSKMEQNPLKMWLCHRIITASHLRFANLLSGTEAFCIYLRLLGAVI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPV DPELI IRT VHLGDFSRIISGFYST GL+RGKIE+Q+N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVLDPELIYIRTGVHLGDFSRIISGFYSTGGLSRGKIEIQDNSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+VILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMH+LDERIEKMD  YKK
Sbjct: 1681 VLPGSVIQEDVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHMLDERIEKMDTKYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNL+ANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGK+YPVFIRHS
Sbjct: 1741 IVGNLSANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SD++PLLDLTLKTGNAFYARTIADFATWLVCGLAAR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDSTPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            EEHVK+VPHIR+AVWNSLRL+DSY+ELHYYSNICRLFRFKDGQEMYVK KLRPYD+TINE
Sbjct: 1861 EEHVKKVPHIRNAVWNSLRLADSYSELHYYSNICRLFRFKDGQEMYVKLKLRPYDRTINE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF SRVNSPGG+RYVFQLQ+RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLSRVNSPGGVRYVFQLQMRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             D+DIALDCTKPWDE EFP +DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 ADQDIALDCTKPWDETEFPLIDIGEIEIHQSLSKEESEALEFNPFLRCDEVDVISATSVS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFL+QSDTKVDLSGCPVAAA+K+R  EKAA
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLQQSDTKVDLSGCPVAAALKKRGKEKAA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+R+W+QNFW+TF QPLLQTALPY+IIGL  FFPLA V+HLKEDKKLPL WLLPL+WVSS
Sbjct: 2101 LDRSWYQNFWLTFCQPLLQTALPYYIIGLATFFPLACVVHLKEDKKLPLHWLLPLMWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GI+AAL CV+AKWVLVQ+KK+GESIGIWS+RIFMDTIWQ I+T+VGDYFMEMT+GSFIF 
Sbjct: 2161 GIMAALGCVVAKWVLVQRKKEGESIGIWSVRIFMDTIWQGIKTVVGDYFMEMTSGSFIFA 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            +IMKLMGSDVDL+QG+YVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 VIMKLMGSDVDLEQGSYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGEGG VGSRAIAMPGVRVESEA+LA LSLAMKEEI+RA+
Sbjct: 2281 EIGEGGFVGSRAIAMPGVRVESEATLAPLSLAMKEEIIRAT 2320

BLAST of MS010231 vs. NCBI nr
Match: TYK21654.1 (Long-chain-fatty-acid--AMP ligase FadD28 [Cucumis melo var. makuwa])

HSP 1 Score: 4106.2 bits (10648), Expect = 0.0e+00
Identity = 2040/2321 (87.89%), Postives = 2175/2321 (93.71%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            MDTGK+LED+FSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGYT VTVLEK+Q VGGM
Sbjct: 1    MDTGKSLEDKFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYTGVTVLEKNQDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS  
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSSN 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+ELF
Sbjct: 241  SVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG P+F+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPVFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSEL 1140
            AIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSEL
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENI
Sbjct: 1141 TNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY SI
Sbjct: 1201 LAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP
Sbjct: 1261 SAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITD
Sbjct: 1321 EVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGV IAEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EVP
Sbjct: 1381 PSLVSIGDGVAIAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYFY
Sbjct: 1441 ALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
            LCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA +
Sbjct: 1561 LCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAKI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+V+LGALSV+PMNSTLI+GGVYVGSRTPVMIKNTMH+LDERIEKMD IYKK
Sbjct: 1681 VLPGSVIQEDVVLGALSVSPMNSTLIRGGVYVGSRTPVMIKNTMHILDERIEKMDKIYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNLAANLAATTLKVKTRYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRHS
Sbjct: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL AR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            E+HVK+VPHIRDAVWNSLRL+ SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+E
Sbjct: 1861 EQHVKKVPHIRDAVWNSLRLTKSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 AERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSIS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK A
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKTA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+RTW+QN W+TF QP LQTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSS
Sbjct: 2101 LDRTWYQNLWLTFFQPTLQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GIIAALCCV+AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFV
Sbjct: 2161 GIIAALCCVVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFV 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            LIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 LIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2321

BLAST of MS010231 vs. NCBI nr
Match: XP_008449759.1 (PREDICTED: uncharacterized protein LOC103491548 isoform X1 [Cucumis melo] >KAA0041362.1 Long-chain-fatty-acid--AMP ligase FadD28 [Cucumis melo var. makuwa])

HSP 1 Score: 4097.7 bits (10626), Expect = 0.0e+00
Identity = 2034/2321 (87.63%), Postives = 2172/2321 (93.58%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            M TGK+LED+FSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGYT VTVLEK+  VGGM
Sbjct: 1    MATGKSLEDKFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYTGVTVLEKNHDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS  
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSSN 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+ELF
Sbjct: 241  SVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG PIF+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSEL 1140
            AIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSEL
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENI
Sbjct: 1141 TNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY SI
Sbjct: 1201 LAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP
Sbjct: 1261 SAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITD
Sbjct: 1321 EVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGV +AEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EVP
Sbjct: 1381 PSLVSIGDGVAVAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYFY
Sbjct: 1441 ALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
            LCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA +
Sbjct: 1561 LCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAKI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+V+LGALSV+PMNSTLI+GG+YVGSRTPVMIKNTMH+LDERIEKMD IYKK
Sbjct: 1681 VLPGSVIQEDVVLGALSVSPMNSTLIRGGIYVGSRTPVMIKNTMHILDERIEKMDKIYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNLAANLAATTLKVK RYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRHS
Sbjct: 1741 IVGNLAANLAATTLKVKARYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL AR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            E+HVK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+E
Sbjct: 1861 EQHVKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 AERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSIS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK A
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKTA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+RTW+QN W+TF QP  QTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSS
Sbjct: 2101 LDRTWYQNLWLTFFQPTFQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GIIAALCC++AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFV
Sbjct: 2161 GIIAALCCIVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFV 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            LIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 LIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2321

BLAST of MS010231 vs. NCBI nr
Match: XP_008449760.1 (PREDICTED: uncharacterized protein LOC103491548 isoform X2 [Cucumis melo])

HSP 1 Score: 3998.0 bits (10367), Expect = 0.0e+00
Identity = 1983/2262 (87.67%), Postives = 2117/2262 (93.59%), Query Frame = 0

Query: 60   MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGV 119
            MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI V
Sbjct: 1    MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRV 60

Query: 120  AEDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTAS 179
            A+DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTAS
Sbjct: 61   ADDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTAS 120

Query: 180  GYGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSF 239
            GYGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS 
Sbjct: 121  GYGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSS 180

Query: 240  RSVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKEL 299
             SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+EL
Sbjct: 181  NSVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEEL 240

Query: 300  FSKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSY 359
            FSKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSY
Sbjct: 241  FSKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSY 300

Query: 360  GNSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQG 419
            GNSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQG
Sbjct: 301  GNSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQG 360

Query: 420  SLNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGL 479
            SLNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL
Sbjct: 361  SLNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGL 420

Query: 480  VELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQ 539
             ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQ
Sbjct: 421  GELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQ 480

Query: 540  KLLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIE 599
            KLLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIE
Sbjct: 481  KLLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIE 540

Query: 600  YIAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFAN 659
            YIAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFAN
Sbjct: 541  YIAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFAN 600

Query: 660  LA-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL 719
            L  P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL
Sbjct: 601  LTPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL 660

Query: 720  VSWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAF 779
            VSWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAF
Sbjct: 661  VSWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAF 720

Query: 780  ELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGL 839
            ELVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGL
Sbjct: 721  ELVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGL 780

Query: 840  AENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIW 899
            AENCVFVSCAFGEG PIF+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIW
Sbjct: 781  AENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIW 840

Query: 900  ISSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAA 959
            ISSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAA
Sbjct: 841  ISSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAA 900

Query: 960  GRNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDG 1019
            GRNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDG
Sbjct: 901  GRNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDG 960

Query: 1020 KPVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP 1079
            KP+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP
Sbjct: 961  KPIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP 1020

Query: 1080 EAIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSE 1139
            EAIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSE
Sbjct: 1021 EAIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSE 1080

Query: 1140 LTNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISEN 1199
            LTNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISEN
Sbjct: 1081 LTNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISEN 1140

Query: 1200 ILNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFS 1259
            IL K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY S
Sbjct: 1141 ILAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLS 1200

Query: 1260 ISAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT 1319
            ISAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT
Sbjct: 1201 ISAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT 1260

Query: 1320 PEVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDIT 1379
            PEVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DIT
Sbjct: 1261 PEVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDIT 1320

Query: 1380 DPSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEV 1439
            DPSLVSIGDGV +AEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EV
Sbjct: 1321 DPSLVSIGDGVAVAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEV 1380

Query: 1440 PALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYF 1499
            PALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYF
Sbjct: 1381 PALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYF 1440

Query: 1500 YIWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGI 1559
            YIWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGI
Sbjct: 1441 YIWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGI 1500

Query: 1560 ILCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAV 1619
            ILCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA 
Sbjct: 1501 ILCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAK 1560

Query: 1620 VGKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQS 1679
            +GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS
Sbjct: 1561 IGKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQS 1620

Query: 1680 TVLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYK 1739
             VLPGSV+QE+V+LGALSV+PMNSTLI+GG+YVGSRTPVMIKNTMH+LDERIEKMD IYK
Sbjct: 1621 IVLPGSVIQEDVVLGALSVSPMNSTLIRGGIYVGSRTPVMIKNTMHILDERIEKMDKIYK 1680

Query: 1740 KIVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRH 1799
            KIVGNLAANLAATTLKVK RYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRH
Sbjct: 1681 KIVGNLAANLAATTLKVKARYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRH 1740

Query: 1800 SNSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAA 1859
            SNSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL A
Sbjct: 1741 SNSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPA 1800

Query: 1860 REEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTIN 1919
            RE+HVK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+
Sbjct: 1801 REQHVKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTID 1860

Query: 1920 EDSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHG 1979
            EDSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   
Sbjct: 1861 EDSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQD 1920

Query: 1980 EVDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSA 2039
            E +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS 
Sbjct: 1921 EAERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSI 1980

Query: 2040 SQSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKA 2099
            SQSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK 
Sbjct: 1981 SQSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKT 2040

Query: 2100 ALNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVS 2159
            AL+RTW+QN W+TF QP  QTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVS
Sbjct: 2041 ALDRTWYQNLWLTFFQPTFQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVS 2100

Query: 2160 SGIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIF 2219
            SGIIAALCC++AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIF
Sbjct: 2101 SGIIAALCCIVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIF 2160

Query: 2220 VLIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGK 2279
            VLIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG 
Sbjct: 2161 VLIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGN 2220

Query: 2280 IEIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            IEIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2221 IEIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2262

BLAST of MS010231 vs. ExPASy Swiss-Prot
Match: B2HIM0 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD28 PE=3 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 2.8e-66
Identity = 175/547 (31.99%), Postives = 279/547 (51.01%), Query Frame = 0

Query: 552  GDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEYIAKSCGAVAIL 611
            GDR +++   GL+++ AF G L+A  I VP+  P    +GG +  +   + +    VAIL
Sbjct: 62   GDRAVILAPQGLEYVVAFLGALQAGRIAVPLSVP----QGGASDERATSVLRDASPVAIL 121

Query: 612  STLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANLAPEVVANQSEP 671
            +T              +I  + ++  + +  P    +  D       +L     +     
Sbjct: 122  TT------------SPVIDDVTQHVSAQSAGPAPSIIELDR-----LDLDAAAGSGAGTE 181

Query: 672  HPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTS------RTVLVSWLPQY 731
            +    ++LQ+TSGST +  GVM++H  L+ N + +   Y + +       + LVSWLP Y
Sbjct: 182  NYPATAYLQYTSGSTREPAGVMLSHQNLVTNFEQLMSGYFADTDGIAPPDSTLVSWLPFY 241

Query: 732  HDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFELVARRL 791
            HDMGL+ G+   ++ G  A+L SP++F++ P  WLQ ++T     SA PNFAFEL A+++
Sbjct: 242  HDMGLVLGVCAPILGGYQAVLTSPVSFLQRPARWLQMLATSSHAFSAAPNFAFELAAKKV 301

Query: 792  EANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAENCVFV 851
              + D +   +L +++ ++  +E V+  TLKRF +      L E+V+ P YGLAE  V+V
Sbjct: 302  --SDDDMAGLDLGNVLTILSGSERVQPATLKRFADRFARFNLQEKVLRPSYGLAEATVYV 361

Query: 852  SCAFGEGKPIFIDSQGR-VCCGYVDQDNADID-------------IRTVNPGTGKELEED 911
            S +     P  ++     +  G   Q  +                +R V+P T  E   D
Sbjct: 362  STSRPGQPPELVEFDAESLSTGQAKQCESGAGTQLVSYVLPRSPIVRVVDPDTCTEC-PD 421

Query: 912  GKEGEIWISSPSAGIGYWGREELSQDTFRNEL----QNRPGRRYIRTGDLGRIIDGKLFI 971
            G  GEIW+   +  IGYW + E S+ TF  +L    +  P   ++RTGD G I DGK+FI
Sbjct: 422  GTVGEIWVCGDNVAIGYWNKPEESERTFGGKLASPSEGTPEGPWLRTGDSGFITDGKMFI 481

Query: 972  TGRIKDLIIAAGRNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDC---- 1031
             GRIKDL+I  GRN  P D+E T++   E+ R   CA I VP D   EK + I +     
Sbjct: 482  IGRIKDLLIVYGRNHSPDDIEATIQ---EITR-SRCAAISVPGDRSTEKLVAIIEYRRRG 541

Query: 1032 -SDQVGVVVIAEVKDGKPVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKR 1070
             SDQ  + ++  VK           ++ + ++  HG+SVA + L+ P +I  TTSGK++R
Sbjct: 542  DSDQEAMDMLVAVK----------REVTSALSNSHGLSVADLVLVAPGSIPTTTSGKVRR 570

BLAST of MS010231 vs. ExPASy Swiss-Prot
Match: A0R618 (Long-chain-fatty-acid--AMP ligase FadD32 OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=fadD32 PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.6e-64
Identity = 192/634 (30.28%), Postives = 293/634 (46.21%), Query Frame = 0

Query: 485  VEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLN---EEGSVLCRRTYRELHLNASCIAQKL 544
            ++FPD SS+  ++  W    V  DK+ Y +L+   E   V    T+ +       +A +L
Sbjct: 13   IKFPDGSSIVAHVERWAK--VRGDKLAYRFLDFSTERDGVPRDLTWAQFSARNRAVAARL 72

Query: 545  LSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEYI 604
                +   +PGDR+ ++    LD++ AFFG L A  I VP+  P      G    ++  +
Sbjct: 73   ----QQVTQPGDRVAILCPQNLDYLVAFFGALYAGRIAVPLFDPSEPGHVG----RLHAV 132

Query: 605  AKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSAL-WPKLPWMHTDSWIKNFANL 664
              +C   AIL+T      VR         +   N +   +    +P     +W+      
Sbjct: 133  LDNCHPSAILTTTEAAEGVR-----KFFRTRPANQRPRVIAVDAVPDDVASTWV------ 192

Query: 665  APEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVS 724
                  N  EP    +++LQ+TSGST    GV ITH  L  NV  +    +       +S
Sbjct: 193  ------NPDEPDETTIAYLQYTSGSTRIPTGVQITHLNLATNVVQVIEALEGEEGDRGLS 252

Query: 725  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQ----ATHSAGPNF 784
            WLP +HDMGLI  L   ++ G      +P  F++ P  W++ ++  +     T S  PNF
Sbjct: 253  WLPFFHDMGLITALLAPMI-GHYFTFMTPAAFVRRPERWIRELARKEGDTGGTISVAPNF 312

Query: 785  AFELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGY 844
            AF+  A R    K      +LS++  ++  +EP+   T++RF E   P G   + + P Y
Sbjct: 313  AFDHAAAR-GVPKPGSPPLDLSNVKAVLNGSEPISAATVRRFNEAFGPFGFPPKAIKPSY 372

Query: 845  GLAENCVFVSCAFGEGKP-IFIDSQGRVCCGYVDQDNADIDIRTVNPGTGK--------- 904
            GLAE  +FVS      +P I    + ++  G + + +AD          GK         
Sbjct: 373  GLAEATLFVSTTPSAEEPKIITVDRDQLNSGRIVEVDADSPKAVAQASAGKVGIAEWAVI 432

Query: 905  -------ELEEDGKEGEIWISSPSAGIGYWGREELSQDTFRNELQNRPG----------R 964
                   EL  DG+ GEIWIS  + G GYWG+ E S  TF+N L++R             
Sbjct: 433  VDAESATEL-PDGQVGEIWISGQNMGTGYWGKPEESVATFQNILKSRTNPSHAEGATDDA 492

Query: 965  RYIRTGDLGRIIDGKLFITGRIKDLIIAAGRNIYPADVEKTVESSSELLRPGCCAVIGVP 1024
             ++RTGD G   DG L+ITGR+KDL+I  GRN YP D+E + + +S+ +R G  A   VP
Sbjct: 493  TWVRTGDYGAFYDGDLYITGRVKDLVIIDGRNHYPQDLEYSAQEASKAIRTGYVAAFSVP 552

Query: 1025 EDILMEK-------GIPIPDCSDQVGVVVIAEVKDG--KPVAKDVIDQIQNRVAEEHGVS 1075
             + L ++       GI          +V++AE   G  K     + D I+  +A  HGV+
Sbjct: 553  ANQLPDEVFENAHSGIKRDPDDTSEQLVIVAERAPGAHKLDIGPITDDIRAAIAVRHGVT 612

BLAST of MS010231 vs. ExPASy Swiss-Prot
Match: B2HIN2 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 3.5e-64
Identity = 190/572 (33.22%), Postives = 288/572 (50.35%), Query Frame = 0

Query: 526  TYRELHLNASCIAQKLLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPP 585
            T+ +++  A  +A +L         PGDR+ ++   GL++I AF G L+A  I VP+  P
Sbjct: 42   TWSQVYARACVVADELTMYG----VPGDRVAILAPQGLEYIVAFLGALQAGFIGVPLSTP 101

Query: 586  DPLQRGGQALLKIEYIAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKL 645
                + G    ++  + +    VAIL+T    SAV VG V    +S  ++G+ +    ++
Sbjct: 102  ----QYGVHDERVSAVLRDSQPVAILTT----SAV-VGDVTKYASS--QDGQPAPSVIEV 161

Query: 646  PWMHTDSWIKNFANLAPEVVANQSEPHPDDVS-FLQFTSGSTGDAKGVMITHGGLIHNVK 705
              +  D+         P     Q+ P P   S +LQ+TSGST    GV+++H  +I NV 
Sbjct: 162  DLLDLDT---------PR--PQQALPQPASGSAYLQYTSGSTRTPAGVIVSHENVIANVT 221

Query: 706  LMRRRY-----KSTSRTVLVSWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLW 765
                 Y     K  + T +VSWLP +HDMGLI G+   LV+G TA+L SPM+F++ P  W
Sbjct: 222  QSLYGYFGGPDKFPADTTVVSWLPLFHDMGLILGICAPLVTGCTAVLLSPMSFLRRPARW 281

Query: 766  LQTISTYQATHSAGPNFAFELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFI 825
            +Q ++++    SA PNFAFEL  RR     + +   +L  ++ ++  +E +   T+KRF 
Sbjct: 282  MQLLASHPKCFSAAPNFAFELAVRR--TTDEDLAGLDLGDVLGIVSGSERIHVATIKRFT 341

Query: 826  ELTIPLGLSEEVMAPGYGLAENCVFV-----------------SCAFGEGKPIFID-SQG 885
            E   P  LS   + P YGLAE  ++V                 S   G  +P   D S G
Sbjct: 342  ERFAPFNLSPAAVRPSYGLAEATLYVAAPEPGTTPRTVRFDYESLTAGHARPCRADGSVG 401

Query: 886  RVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGREELSQDTFRN 945
                 Y   D +   +R VNP T  E    G  GEIW       +GYW + E S  TF  
Sbjct: 402  TELISYGSPDPS--AVRIVNPETMIE-NPSGTVGEIWAHGEHVAMGYWQKPEQSDRTFNA 461

Query: 946  ELQN----RPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAGRNIYPADVEKTVESSSEL 1005
             + N     P   ++RTGDLG + +G+LFI GRIKDL+I  GRN YP D+E T++     
Sbjct: 462  RIVNPAPGTPEGPWLRTGDLGVMSNGELFIMGRIKDLVIVDGRNHYPDDIEATIQE---- 521

Query: 1006 LRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGKPVAKDVIDQIQNRVAEEH 1065
            +  G  A I VP++I  E+ + I +   +      A VK      + V  +I + +++ H
Sbjct: 522  ITGGRVAAIAVPDNI-TEQLVAIIELKRRGASAEEAMVK-----LRSVKREITSAISKSH 572

Query: 1066 GVSVASVKLIKPRTISKTTSGKIKRFECLKQF 1070
             + VA V L+ P +I  TTSGKI+R  C++++
Sbjct: 582  SLRVADVVLVPPGSIPITTSGKIRRAACVERY 572

BLAST of MS010231 vs. ExPASy Swiss-Prot
Match: B2HMK0 (Long-chain-fatty-acid--AMP ligase FadD32 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD32 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.9e-64
Identity = 198/638 (31.03%), Postives = 301/638 (47.18%), Query Frame = 0

Query: 485  VEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLN---EEGSVLCRRTYRELHLNASCIAQKL 544
            + FP+ ++L  ++  W    V  DK+ Y +L+   E   V     + E       +  +L
Sbjct: 13   IRFPENTNLVRHVEKWA--RVRGDKLAYRFLDFSTERDGVERDILWSEFSARNRAVGARL 72

Query: 545  LSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEYI 604
                +   +PGDR+ ++    LD++ +FFG L +  I VP+   DP + G     ++  +
Sbjct: 73   ----QQVTQPGDRIAILCPQNLDYLISFFGALYSGRIAVPLF--DPAEPGHVG--RLHAV 132

Query: 605  AKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANLA 664
               C    IL+T      VR       I S     +S+   P++  +  D+       + 
Sbjct: 133  LDDCTPSTILTTTDSAEGVR-----KFIRS-----RSAKERPRV--IAVDA-------VP 192

Query: 665  PEVVANQSEPHPDDV--SFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 724
             EV +   +P  +++  ++LQ+TSGST    GV ITH  L  NV  +    +       V
Sbjct: 193  TEVASTWQQPEANELTTAYLQYTSGSTRVPSGVQITHLNLPTNVLQVLNALEGQEGDRGV 252

Query: 725  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQ----ATHSAGPN 784
            SWLP +HDMGLI  L  A V G +    +P  F++ P  W++ ++        T SA PN
Sbjct: 253  SWLPFFHDMGLITVLL-ASVLGHSFTFMTPAAFVRRPGRWIRELARKPGETGGTFSAAPN 312

Query: 785  FAFELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPG 844
            FAFE  A R    +D     +LS++  ++  +EPV   ++++F +   P GL E  + P 
Sbjct: 313  FAFEHAAMR-GVPRDDEPPLDLSNVKGILNGSEPVSPASMRKFFKAFEPYGLRETAVKPS 372

Query: 845  YGLAENCVFVSCAFGEGKPIFI--------------------DSQGRVCCGYVDQDNADI 904
            YGLAE  +FVS    +  P  I                    ++  +V  G V  D   +
Sbjct: 373  YGLAEATLFVSTTPMDEVPTVIHVDRDELNKQRFVEVAADAPNAVAQVSAGKVGVDEWAV 432

Query: 905  DIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGREELSQDTFRNELQNRPGRR----- 964
                V+  T  EL  DG+ GEIW+   + GIGYWG+EE S  TFRN L++R         
Sbjct: 433  ---IVDTETASEL-PDGQIGEIWLHGNNLGIGYWGKEEESAQTFRNILKSRVPESHAEGA 492

Query: 965  -----YIRTGDLGRIIDGKLFITGRIKDLIIAAGRNIYPADVEKTVESSSELLRPGCCAV 1024
                 ++RTGD G    G L+I GRIKDL+I  GRN YP D+E T + S++ LR G  A 
Sbjct: 493  PDDGLWVRTGDYGTYFKGHLYIAGRIKDLVIIDGRNHYPQDLEYTAQESTKALRVGYVAA 552

Query: 1025 IGVPEDILMEKGIPIP---------DCSDQVGVVVIAEVKDGKPVAKDVIDQIQNRVAEE 1075
              VP + L +K    P         D S+Q+ +V        K   + + D I+  +A  
Sbjct: 553  FSVPANQLPQKVFDDPHAGLSFDPEDTSEQLVIVGERAAGTHKLEYQPIADDIRAAIAVG 612

BLAST of MS010231 vs. ExPASy Swiss-Prot
Match: Q7TXM1 (Long-chain-fatty-acid--AMP ligase FadD26 OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=fadD26 PE=1 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.5e-62
Identity = 186/577 (32.24%), Postives = 287/577 (49.74%), Query Frame = 0

Query: 526  TYRELHLNASCIAQKLLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPP 585
            T+ +++  A  IA++L    K    PGDR+ ++   GL+++ AF G L+A  I VP+  P
Sbjct: 42   TWSQVYSRACIIAEEL----KLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTP 101

Query: 586  DPLQRGGQALLKIEYIAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKL 645
                + G    ++  + +    VAIL+T    S+V VG V    AS   +G+ + +  ++
Sbjct: 102  ----QYGIHDDRVSAVLQDSKPVAILTT----SSV-VGDVTKYAAS--HDGQPAPVVVEV 161

Query: 646  PWMHTDSWIKNFANLAPEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKL 705
              +  DS         P  +   S  H    ++LQ+TSGST    GV+++H  +I NV  
Sbjct: 162  DLLDLDS---------PRQMPAFSRQH-TGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQ 221

Query: 706  MRRRYKSTSRTV----LVSWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQ 765
                Y      +    +VSWLP YHDMGLI G+   LV+   A+L SPM+F++ P  W+Q
Sbjct: 222  SMYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAVLMSPMSFLRRPARWMQ 281

Query: 766  TISTYQATHSAGPNFAFELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIEL 825
             ++T     SA PNFAFEL  RR   +   +   +L  +V ++  +E +   T++RFIE 
Sbjct: 282  LLATSGRCFSAAPNFAFELAVRR--TSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIER 341

Query: 826  TIPLGLSEEVMAPGYGLAENCVFVSC-----------------AFGEGKPIFID-SQGRV 885
              P  LS   + P YGLAE  ++V+                    G+ +P   D S G  
Sbjct: 342  FAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTE 401

Query: 886  CCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGREELSQDTFRNEL 945
               Y   D +   +R VNP T  E    G  GEIW+      +GYW + + +   F  +L
Sbjct: 402  LISYGSPDPS--SVRIVNPETMVE-NPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKL 461

Query: 946  QN----RPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAGRNIYPADVEKTVESSSELLR 1005
             +     P   ++RTGDLG I DG+LFI GRIKDL+I  GRN YP D+E T++     + 
Sbjct: 462  VDPAPAAPEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQE----IT 521

Query: 1006 PGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGKPVAKDVI-------DQIQNR 1065
             G  A I VP+DI  +             +V I E K     A++V+        ++ + 
Sbjct: 522  GGRAAAIAVPDDITEQ-------------LVAIIEFKRRGSTAEEVMLKLRSVKREVTSA 571

Query: 1066 VAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQF 1070
            +++ H + VA + L+ P +I  TTSGKI+R  C++++
Sbjct: 582  ISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERY 571

BLAST of MS010231 vs. ExPASy TrEMBL
Match: A0A5D3DDI8 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00160 PE=4 SV=1)

HSP 1 Score: 4106.2 bits (10648), Expect = 0.0e+00
Identity = 2040/2321 (87.89%), Postives = 2175/2321 (93.71%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            MDTGK+LED+FSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGYT VTVLEK+Q VGGM
Sbjct: 1    MDTGKSLEDKFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYTGVTVLEKNQDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS  
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSSN 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+ELF
Sbjct: 241  SVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG P+F+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPVFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSEL 1140
            AIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSEL
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENI
Sbjct: 1141 TNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY SI
Sbjct: 1201 LAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP
Sbjct: 1261 SAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITD
Sbjct: 1321 EVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGV IAEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EVP
Sbjct: 1381 PSLVSIGDGVAIAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYFY
Sbjct: 1441 ALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
            LCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA +
Sbjct: 1561 LCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAKI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+V+LGALSV+PMNSTLI+GGVYVGSRTPVMIKNTMH+LDERIEKMD IYKK
Sbjct: 1681 VLPGSVIQEDVVLGALSVSPMNSTLIRGGVYVGSRTPVMIKNTMHILDERIEKMDKIYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNLAANLAATTLKVKTRYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRHS
Sbjct: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL AR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            E+HVK+VPHIRDAVWNSLRL+ SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+E
Sbjct: 1861 EQHVKKVPHIRDAVWNSLRLTKSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 AERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSIS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK A
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKTA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+RTW+QN W+TF QP LQTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSS
Sbjct: 2101 LDRTWYQNLWLTFFQPTLQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GIIAALCCV+AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFV
Sbjct: 2161 GIIAALCCVVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFV 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            LIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 LIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2321

BLAST of MS010231 vs. ExPASy TrEMBL
Match: A0A5A7TD32 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00070 PE=4 SV=1)

HSP 1 Score: 4097.7 bits (10626), Expect = 0.0e+00
Identity = 2034/2321 (87.63%), Postives = 2172/2321 (93.58%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            M TGK+LED+FSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGYT VTVLEK+  VGGM
Sbjct: 1    MATGKSLEDKFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYTGVTVLEKNHDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS  
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSSN 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+ELF
Sbjct: 241  SVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG PIF+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSEL 1140
            AIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSEL
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENI
Sbjct: 1141 TNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY SI
Sbjct: 1201 LAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP
Sbjct: 1261 SAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITD
Sbjct: 1321 EVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGV +AEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EVP
Sbjct: 1381 PSLVSIGDGVAVAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYFY
Sbjct: 1441 ALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
            LCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA +
Sbjct: 1561 LCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAKI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+V+LGALSV+PMNSTLI+GG+YVGSRTPVMIKNTMH+LDERIEKMD IYKK
Sbjct: 1681 VLPGSVIQEDVVLGALSVSPMNSTLIRGGIYVGSRTPVMIKNTMHILDERIEKMDKIYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNLAANLAATTLKVK RYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRHS
Sbjct: 1741 IVGNLAANLAATTLKVKARYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL AR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            E+HVK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+E
Sbjct: 1861 EQHVKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 AERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSIS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK A
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKTA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+RTW+QN W+TF QP  QTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSS
Sbjct: 2101 LDRTWYQNLWLTFFQPTFQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GIIAALCC++AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFV
Sbjct: 2161 GIIAALCCIVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFV 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            LIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 LIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2321

BLAST of MS010231 vs. ExPASy TrEMBL
Match: A0A1S3BNF2 (uncharacterized protein LOC103491548 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491548 PE=4 SV=1)

HSP 1 Score: 4097.7 bits (10626), Expect = 0.0e+00
Identity = 2034/2321 (87.63%), Postives = 2172/2321 (93.58%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            M TGK+LED+FSKLHPSLPLNT+IGIIGGGPSGLSAAYALAKLGYT VTVLEK+  VGGM
Sbjct: 1    MATGKSLEDKFSKLHPSLPLNTRIGIIGGGPSGLSAAYALAKLGYTGVTVLEKNHDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS  
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSSN 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+ELF
Sbjct: 241  SVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG PIF+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSEL 1140
            AIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSEL
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSEL 1140

Query: 1141 TNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENI 1200
            TNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENI
Sbjct: 1141 TNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENI 1200

Query: 1201 LNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSI 1260
            L K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY SI
Sbjct: 1201 LAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLSI 1260

Query: 1261 SAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320
            SAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP
Sbjct: 1261 SAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTP 1320

Query: 1321 EVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITD 1380
            EVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITD
Sbjct: 1321 EVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITD 1380

Query: 1381 PSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVP 1440
            PSLVSIGDGV +AEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EVP
Sbjct: 1381 PSLVSIGDGVAVAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEVP 1440

Query: 1441 ALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFY 1500
            ALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYFY
Sbjct: 1441 ALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYFY 1500

Query: 1501 IWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGII 1560
            IWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGII
Sbjct: 1501 IWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGII 1560

Query: 1561 LCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVV 1620
            LCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA +
Sbjct: 1561 LCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAKI 1620

Query: 1621 GKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQST 1680
            GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS 
Sbjct: 1621 GKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSI 1680

Query: 1681 VLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKK 1740
            VLPGSV+QE+V+LGALSV+PMNSTLI+GG+YVGSRTPVMIKNTMH+LDERIEKMD IYKK
Sbjct: 1681 VLPGSVIQEDVVLGALSVSPMNSTLIRGGIYVGSRTPVMIKNTMHILDERIEKMDKIYKK 1740

Query: 1741 IVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHS 1800
            IVGNLAANLAATTLKVK RYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRHS
Sbjct: 1741 IVGNLAANLAATTLKVKARYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRHS 1800

Query: 1801 NSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAR 1860
            NSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL AR
Sbjct: 1801 NSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAR 1860

Query: 1861 EEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINE 1920
            E+HVK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+E
Sbjct: 1861 EQHVKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDE 1920

Query: 1921 DSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGE 1980
            DSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E
Sbjct: 1921 DSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDE 1980

Query: 1981 VDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSAS 2040
             +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS S
Sbjct: 1981 AERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSIS 2040

Query: 2041 QSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAA 2100
            QSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK A
Sbjct: 2041 QSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKTA 2100

Query: 2101 LNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSS 2160
            L+RTW+QN W+TF QP  QTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSS
Sbjct: 2101 LDRTWYQNLWLTFFQPTFQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSS 2160

Query: 2161 GIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFV 2220
            GIIAALCC++AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFV
Sbjct: 2161 GIIAALCCIVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFV 2220

Query: 2221 LIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKI 2280
            LIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG I
Sbjct: 2221 LIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGNI 2280

Query: 2281 EIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            EIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2321

BLAST of MS010231 vs. ExPASy TrEMBL
Match: A0A0A0KZ76 (Carrier domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G286390 PE=4 SV=1)

HSP 1 Score: 4092.0 bits (10611), Expect = 0.0e+00
Identity = 2030/2318 (87.58%), Postives = 2170/2318 (93.62%), Query Frame = 0

Query: 1    MDTGKTLEDQFSKLHPSLPLNTKIGIIGGGPSGLSAAYALAKLGYTEVTVLEKHQAVGGM 60
            MDTGK+ ED+FSKLHPSLPLNT+ GIIGGGPSGLSAAYALAKLGY EVTVLEK+Q VGGM
Sbjct: 1    MDTGKSFEDKFSKLHPSLPLNTRFGIIGGGPSGLSAAYALAKLGYNEVTVLEKNQDVGGM 60

Query: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGVA 120
            CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI VA
Sbjct: 61   CESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRVA 120

Query: 121  EDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTASG 180
            +DY S IS+TLELQDKAKDSG+IGVHAVS FASDLTP YLEAHGLTSVPKSVAYGYTASG
Sbjct: 121  DDYTSSISLTLELQDKAKDSGYIGVHAVSAFASDLTPTYLEAHGLTSVPKSVAYGYTASG 180

Query: 181  YGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSFR 240
            YGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS +
Sbjct: 181  YGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCQTEVVSVRRSSK 240

Query: 241  SVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKELF 300
            SV L VMDRD+N TSLEFDK+IISGSFPF++ RTYRSS+ KLSEE  E+MDMSHLE+ELF
Sbjct: 241  SVTLRVMDRDKNFTSLEFDKIIISGSFPFRNCRTYRSSSPKLSEEEAEVMDMSHLEEELF 300

Query: 301  SKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSYG 360
            SKVYTIDYYTTVLKI+GLNHLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSYG
Sbjct: 301  SKVYTIDYYTTVLKIDGLNHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSYG 360

Query: 361  NSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQGS 420
            NSADITGPNVTELA+NT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQGS
Sbjct: 361  NSADITGPNVTELAMNTIAKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQGS 420

Query: 421  LNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGLV 480
            LNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSS  FSYAKPMFL QSK E+   GL 
Sbjct: 421  LNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSLTFSYAKPMFLFQSKPERDAMGLG 480

Query: 481  ELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQK 540
            ELPGVEFP+LSSLDGYLRHWGSHHVT+D+VLY WLNEEG VL +RTYRELHLNASCIAQK
Sbjct: 481  ELPGVEFPNLSSLDGYLRHWGSHHVTQDRVLYNWLNEEGLVLGQRTYRELHLNASCIAQK 540

Query: 541  LLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIEY 600
            LLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIEY
Sbjct: 541  LLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIEY 600

Query: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFANL 660
            IAKSCGAVAILSTLGYHSAVRVGKVKNMI  +RE GKSSA+WPKLPWMHTDSWIKNFANL
Sbjct: 601  IAKSCGAVAILSTLGYHSAVRVGKVKNMIGLMREKGKSSAVWPKLPWMHTDSWIKNFANL 660

Query: 661  A-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLV 720
              P+ +A+QS+PHPD+VSFLQFTSGSTGDAKGV+ITHGGLIHNVKLMRRRYKSTSRTVLV
Sbjct: 661  TPPDTMADQSDPHPDEVSFLQFTSGSTGDAKGVVITHGGLIHNVKLMRRRYKSTSRTVLV 720

Query: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFE 780
            SWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAFE
Sbjct: 721  SWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAFE 780

Query: 781  LVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLA 840
            LVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT P GL+EE MAPGYGLA
Sbjct: 781  LVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTAPFGLTEEKMAPGYGLA 840

Query: 841  ENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWI 900
            ENCVFVSCAFGEG PIF+D QGRVCCGYVDQDNADIDIR VNPGTGKELEEDGKEGEIWI
Sbjct: 841  ENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNADIDIRIVNPGTGKELEEDGKEGEIWI 900

Query: 901  SSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAAG 960
            SSPSAGIGYWGREELSQ+TFRNELQN PGRRYIRTGDLGR+IDGKLFITGRIKDLIIAAG
Sbjct: 901  SSPSAGIGYWGREELSQETFRNELQNHPGRRYIRTGDLGRVIDGKLFITGRIKDLIIAAG 960

Query: 961  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGK 1020
            RNIYPADVEKTVESSS+LLRPGCCAV+GVPE+ILMEKGI +PDCSDQVG+VVIAEVKDGK
Sbjct: 961  RNIYPADVEKTVESSSDLLRPGCCAVVGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDGK 1020

Query: 1021 PVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPE 1080
            P+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP+
Sbjct: 1021 PIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVPD 1080

Query: 1081 AIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRASVQPSPRISNKDIEEFLKGLVSELTNI 1140
            AIKLRRTFLRSFSTGTCKEG TPR Q +NLSRASVQP PRISN+DIEEFLKGLVSELTNI
Sbjct: 1081 AIKLRRTFLRSFSTGTCKEGITPRFQQTNLSRASVQPDPRISNRDIEEFLKGLVSELTNI 1140

Query: 1141 PINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISENILNK 1200
            PINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISENIL K
Sbjct: 1141 PINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISENILAK 1200

Query: 1201 NHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFSISAF 1260
            + AQSTKN  N TFET CAL+ ME IS T QF IWFF LLALIFVA+M++FPAY S+SAF
Sbjct: 1201 SRAQSTKNTTNLTFETNCALVRMETISWTHQFGIWFFQLLALIFVAMMVIFPAYLSMSAF 1260

Query: 1261 TSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTPEVS 1320
             SS+ ILH LTD I LMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTPEVS
Sbjct: 1261 LSSLPILHDLTDNIYLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALTPEVS 1320

Query: 1321 IWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDITDPSL 1380
            IWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DITDPSL
Sbjct: 1321 IWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDITDPSL 1380

Query: 1381 VSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEVPALQ 1440
            VSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGA LGEE EVPALQ
Sbjct: 1381 VSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGAILGEEVEVPALQ 1440

Query: 1441 KIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYFYIWL 1500
            +IEGI  TS N+  +K S+P+   G+RQE + IYHFLGIYL+GFLGSLSAAIVYYFYIWL
Sbjct: 1441 RIEGIETTSGNRCFEKGSEPRRTGGERQERDTIYHFLGIYLLGFLGSLSAAIVYYFYIWL 1500

Query: 1501 SQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGIILCI 1560
            SQSSPSLQH AF+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGIILCI
Sbjct: 1501 SQSSPSLQHFAFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGIILCI 1560

Query: 1561 LTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAVVGKH 1620
            LTF +K LLT+KP MEQ PLKIWLCHRI  ASHLRFAKLLSGTEAFCIYLRLLGA +GKH
Sbjct: 1561 LTFFVKKLLTSKPQMEQTPLKIWLCHRIITASHLRFAKLLSGTEAFCIYLRLLGAKIGKH 1620

Query: 1621 CSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQSTVLP 1680
            CSIRAINPVSDPELISIR  VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS VLP
Sbjct: 1621 CSIRAINPVSDPELISIRMGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQSIVLP 1680

Query: 1681 GSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYKKIVG 1740
            GS++QE+V+LGALSV+PMNSTLI+GGVYVGSRTPVMIKNTMH+LDERIEKMD IYKKIVG
Sbjct: 1681 GSLIQEDVVLGALSVSPMNSTLIRGGVYVGSRTPVMIKNTMHILDERIEKMDRIYKKIVG 1740

Query: 1741 NLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRHSNSL 1800
            NLAANLAATTLKVK RYFHRIGVSGKG+L IYD+IKGLPDHKIFS GK+YPVFIRHSNSL
Sbjct: 1741 NLAANLAATTLKVKRRYFHRIGVSGKGYLTIYDDIKGLPDHKIFSPGKSYPVFIRHSNSL 1800

Query: 1801 SADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAAREEH 1860
            SADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL ARE+H
Sbjct: 1801 SADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPAREQH 1860

Query: 1861 VKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTINEDSG 1920
            VK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+EDSG
Sbjct: 1861 VKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTIDEDSG 1920

Query: 1921 KVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHGEVDR 1980
            KVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   E ++
Sbjct: 1921 KVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQDEAEQ 1980

Query: 1981 DIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSASQSA 2040
            DIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS SQSA
Sbjct: 1981 DIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSISQSA 2040

Query: 2041 SIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKAALNR 2100
            SIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK AL+R
Sbjct: 2041 SIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERSNEKTALDR 2100

Query: 2101 TWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVSSGII 2160
            TW+QN W+TF QP LQTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVSSGII
Sbjct: 2101 TWYQNLWLTFFQPTLQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVSSGII 2160

Query: 2161 AALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIFVLIM 2220
            AALCCV+AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIFVLIM
Sbjct: 2161 AALCCVVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIFVLIM 2220

Query: 2221 KLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGKIEIG 2280
            KLMGSDVD++QGTYVDSMGALLNPEMV++H+GGSVGREALLFGHIYEGGG VKFG IEIG
Sbjct: 2221 KLMGSDVDMNQGTYVDSMGALLNPEMVRVHRGGSVGREALLFGHIYEGGGVVKFGNIEIG 2280

Query: 2281 EGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            E G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2281 EDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2318

BLAST of MS010231 vs. ExPASy TrEMBL
Match: A0A1S3BNQ6 (uncharacterized protein LOC103491548 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491548 PE=4 SV=1)

HSP 1 Score: 3998.0 bits (10367), Expect = 0.0e+00
Identity = 1983/2262 (87.67%), Postives = 2117/2262 (93.59%), Query Frame = 0

Query: 60   MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEEMDSHKLALIDTTGKYQDIGV 119
            MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEE+DSHKLALIDT+G+YQDI V
Sbjct: 1    MCESVEIEGKIYDLGGQVLAANSAPTIFHLAKETGSELEELDSHKLALIDTSGEYQDIRV 60

Query: 120  AEDYKSIISVTLELQDKAKDSGHIGVHAVSEFASDLTPAYLEAHGLTSVPKSVAYGYTAS 179
            A+DY SIIS+TL+LQDKAKDSGHIGVHAVS FASDLTP YLEA GLTSVPKSVAYGYTAS
Sbjct: 61   ADDYTSIISLTLQLQDKAKDSGHIGVHAVSAFASDLTPTYLEARGLTSVPKSVAYGYTAS 120

Query: 180  GYGFLQDMPYAYAHEFTRTSMAGKIRRFRRGYGGFWKKISESLPIKVHCNTEVVSVRRSF 239
            GYGF+QDMPYAY HEFTRTSMAGKIRRF+ GYGG WK+ISESLPIKVHC TEVVSVRRS 
Sbjct: 121  GYGFVQDMPYAYVHEFTRTSMAGKIRRFKGGYGGLWKRISESLPIKVHCKTEVVSVRRSS 180

Query: 240  RSVILHVMDRDRNLTSLEFDKVIISGSFPFKSGRTYRSSTTKLSEEGVEIMDMSHLEKEL 299
             SV L VMDRD+NL SLEFDK+IISGSFPF++ RTYRSS+ KLSEEG E+MDMS LE+EL
Sbjct: 181  NSVTLRVMDRDKNLASLEFDKIIISGSFPFRNCRTYRSSSPKLSEEGAEVMDMSRLEEEL 240

Query: 300  FSKVYTIDYYTTVLKIEGLNHLPLGFYYFGKHMDNPETIGYPVAMQRFYPDTDIFLFWSY 359
            FSKVYTIDYYTTVLKI+GL+HLPLGFYYFGKHMDNPETIGYPVAMQ+FYPDT+IFL WSY
Sbjct: 241  FSKVYTIDYYTTVLKIDGLDHLPLGFYYFGKHMDNPETIGYPVAMQKFYPDTNIFLLWSY 300

Query: 360  GNSADITGPNVTELAINTVKTMGGEVKKVILQRRFKYFPHVCSKDMEDGFYRRLELELQG 419
            GNSADITGPNVT LAINT+  MGGEVKKVILQRRFKYFPHVCSKDMEDGFY+RLELELQG
Sbjct: 301  GNSADITGPNVTALAINTITKMGGEVKKVILQRRFKYFPHVCSKDMEDGFYKRLELELQG 360

Query: 420  SLNTYYVGGLMAFELTERNSSYAMSLVCKHFANNNSSPMFSYAKPMFLLQSKREKVLKGL 479
            SLNTYY GGLMAFELTERNSSYAM+LVCKHFAN+NSSP FSYAKPMFL QSK+E+  KGL
Sbjct: 361  SLNTYYTGGLMAFELTERNSSYAMALVCKHFANDNSSPTFSYAKPMFLFQSKQERDAKGL 420

Query: 480  VELPGVEFPDLSSLDGYLRHWGSHHVTRDKVLYTWLNEEGSVLCRRTYRELHLNASCIAQ 539
             ELPGVEFP+LSSLDGYLRHWGSHHVTRD+VLYTWLNEEGSVL +RTYRELHLNASCIAQ
Sbjct: 421  GELPGVEFPNLSSLDGYLRHWGSHHVTRDRVLYTWLNEEGSVLGQRTYRELHLNASCIAQ 480

Query: 540  KLLSNQKPPIKPGDRLLLIYVPGLDFIDAFFGCLRAKVIPVPVLPPDPLQRGGQALLKIE 599
            KLLSNQKP IKPGDR+LLIYVPGLDFIDAFFGCLRAKV+PVPVLPPDPLQRGGQALLKIE
Sbjct: 481  KLLSNQKPSIKPGDRVLLIYVPGLDFIDAFFGCLRAKVLPVPVLPPDPLQRGGQALLKIE 540

Query: 600  YIAKSCGAVAILSTLGYHSAVRVGKVKNMIASIRENGKSSALWPKLPWMHTDSWIKNFAN 659
            YIAKSC AVAILSTL YHSAVRVGKVKNMI   RENGKSSA+WPKLPWMHTDSWIKNFAN
Sbjct: 541  YIAKSCSAVAILSTLSYHSAVRVGKVKNMIGLTRENGKSSAVWPKLPWMHTDSWIKNFAN 600

Query: 660  LA-PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL 719
            L  P+ +A+QS+PHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL
Sbjct: 601  LTPPDTMADQSDPHPDDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVL 660

Query: 720  VSWLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAF 779
            VSWLPQYHDMGLIGGLFTALVSGGTAILFSP+TFIKNPLLWL T+S Y+ATHSAGPNFAF
Sbjct: 661  VSWLPQYHDMGLIGGLFTALVSGGTAILFSPLTFIKNPLLWLHTMSKYKATHSAGPNFAF 720

Query: 780  ELVARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGL 839
            ELVARRLE NK KVQ Y+LSS+VFLM+AAEPVRK TLK+F+ELT+P GL+EE MAPGYGL
Sbjct: 721  ELVARRLEVNKGKVQTYDLSSMVFLMIAAEPVRKTTLKKFLELTVPFGLTEEKMAPGYGL 780

Query: 840  AENCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIW 899
            AENCVFVSCAFGEG PIF+D QGRVCCGYVDQDN DIDIR VNPGTGKELEEDGKEGEIW
Sbjct: 781  AENCVFVSCAFGEGIPIFVDWQGRVCCGYVDQDNTDIDIRIVNPGTGKELEEDGKEGEIW 840

Query: 900  ISSPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRIIDGKLFITGRIKDLIIAA 959
            ISSPSAGIGYWGREELSQ+TFRNELQN PGRRY RTGDLGR+IDGKLFITGRIKDLIIAA
Sbjct: 841  ISSPSAGIGYWGREELSQETFRNELQNHPGRRYTRTGDLGRVIDGKLFITGRIKDLIIAA 900

Query: 960  GRNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDG 1019
            GRNIYPADVEKTVESSS+LLRPGCCAVIGVPE+ILMEKGI +PDCSDQVG+VVIAEVKDG
Sbjct: 901  GRNIYPADVEKTVESSSDLLRPGCCAVIGVPEEILMEKGIQVPDCSDQVGLVVIAEVKDG 960

Query: 1020 KPVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP 1079
            KP+AKD+IDQIQNRVAEEHGVSVAS+KLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP
Sbjct: 961  KPIAKDIIDQIQNRVAEEHGVSVASIKLIKPRTISKTTSGKIKRFECLKQFVDGTLNVVP 1020

Query: 1080 EAIKLRRTFLRSFSTGTCKEGNTPRPQLSNLSRA---SVQPSPRISNKDIEEFLKGLVSE 1139
            EAIKLRRTFLRSFSTGTCKEG TPRPQ + LSRA   SVQP  RISN+DIEEFLKGLVSE
Sbjct: 1021 EAIKLRRTFLRSFSTGTCKEGITPRPQQTKLSRASVPSVQPGLRISNRDIEEFLKGLVSE 1080

Query: 1140 LTNIPINKICATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIAELASISEN 1199
            LTNI INKI ATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIA+LASISEN
Sbjct: 1081 LTNISINKISATESLLSYGIDSILVVRAAQKLSNFLGVPVGAVDIFTASCIADLASISEN 1140

Query: 1200 ILNKNHAQSTKNPANPTFETACALIEMEKISRTRQFAIWFFHLLALIFVAVMLVFPAYFS 1259
            IL K+HAQSTKN  NPT++T C L+ ME +S TRQF IWF  LLALIFVA+M++FPAY S
Sbjct: 1141 ILAKSHAQSTKNTTNPTYDTNCVLVGMETVSWTRQFVIWFSQLLALIFVAMMVLFPAYLS 1200

Query: 1260 ISAFTSSISILHKLTDEIPLMNYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT 1319
            ISAFTSS+ ILH LTD I LM+YLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT
Sbjct: 1201 ISAFTSSLPILHNLTDNISLMDYLLPLTLAPLAWILCIVSSCICIAFFGNSFLRPNYALT 1260

Query: 1320 PEVSIWSIDFVKWWAMYKAQDVSSKVLAVHLRGTVFLKFWYEMFGARIGSSVILDTIDIT 1379
            PEVSIWS+DFVKWWA+YKAQDVSSKVLAVHLRGTVFLK+WYEMFGARIGSSVILDT+DIT
Sbjct: 1261 PEVSIWSLDFVKWWALYKAQDVSSKVLAVHLRGTVFLKYWYEMFGARIGSSVILDTVDIT 1320

Query: 1380 DPSLVSIGDGVVIAEGALIQSHEVKNGVLSFLPIRIGQNSSVGPYASIHKGATLGEEAEV 1439
            DPSLVSIGDGV +AEGALIQSHEVKNGVLSFLPIRIG+NSSVGPYASIHKGA LGEE EV
Sbjct: 1321 DPSLVSIGDGVAVAEGALIQSHEVKNGVLSFLPIRIGRNSSVGPYASIHKGAILGEEVEV 1380

Query: 1440 PALQKIEGIVKTSDNKNLQKCSKPQSIAGKRQELEAIYHFLGIYLVGFLGSLSAAIVYYF 1499
            PALQ+IEGI  TS N+  +K S+P+  AG+RQE E IYHF GIY++GFLGSLSAAIVYYF
Sbjct: 1381 PALQRIEGIETTSGNRCFEKGSEPRRNAGERQERETIYHFFGIYILGFLGSLSAAIVYYF 1440

Query: 1500 YIWLSQSSPSLQHLAFISLAGTFHWTPFTAIAYATIFAEVPSNATSFAILFSSTYLFHGI 1559
            YIWLSQSSPSLQH +F+ L G FHW PFT IAYATIFAEVPSNA SFAILFSS YLFHGI
Sbjct: 1441 YIWLSQSSPSLQHFSFLCLVGAFHWIPFTVIAYATIFAEVPSNAISFAILFSSMYLFHGI 1500

Query: 1560 ILCILTFILKYLLTTKPNMEQNPLKIWLCHRITIASHLRFAKLLSGTEAFCIYLRLLGAV 1619
            ILCILTF++K LLT+KP MEQ PLKIWLCHRIT ASHLRFAKLLSGTEAFCIYLRLLGA 
Sbjct: 1501 ILCILTFVVKNLLTSKPQMEQTPLKIWLCHRITAASHLRFAKLLSGTEAFCIYLRLLGAK 1560

Query: 1620 VGKHCSIRAINPVSDPELISIRTSVHLGDFSRIISGFYSTNGLTRGKIEVQNNAVIGSQS 1679
            +GKHCSIRAINPVSDPELISIRT VHLGDFSRIISGFYSTNGLTRGKIEVQ N+VIGSQS
Sbjct: 1561 IGKHCSIRAINPVSDPELISIRTGVHLGDFSRIISGFYSTNGLTRGKIEVQENSVIGSQS 1620

Query: 1680 TVLPGSVVQENVILGALSVAPMNSTLIKGGVYVGSRTPVMIKNTMHVLDERIEKMDMIYK 1739
             VLPGSV+QE+V+LGALSV+PMNSTLI+GG+YVGSRTPVMIKNTMH+LDERIEKMD IYK
Sbjct: 1621 IVLPGSVIQEDVVLGALSVSPMNSTLIRGGIYVGSRTPVMIKNTMHILDERIEKMDKIYK 1680

Query: 1740 KIVGNLAANLAATTLKVKTRYFHRIGVSGKGHLKIYDNIKGLPDHKIFSAGKTYPVFIRH 1799
            KIVGNLAANLAATTLKVK RYFHRIGVSGKG+LKIYDNIKGLPDHKIFS GK+YPVFIRH
Sbjct: 1681 KIVGNLAANLAATTLKVKARYFHRIGVSGKGYLKIYDNIKGLPDHKIFSPGKSYPVFIRH 1740

Query: 1800 SNSLSADDDARIDARGAALRIFSDESDTSPLLDLTLKTGNAFYARTIADFATWLVCGLAA 1859
            SNSLSADDDARIDARGAALRI SD SDT+PLLDLTLKTGNAFYARTIADFA+WLVCGL A
Sbjct: 1741 SNSLSADDDARIDARGAALRILSDGSDTTPLLDLTLKTGNAFYARTIADFASWLVCGLPA 1800

Query: 1860 REEHVKRVPHIRDAVWNSLRLSDSYAELHYYSNICRLFRFKDGQEMYVKFKLRPYDKTIN 1919
            RE+HVK+VPHIRDAVWNSLRL++SY ELHYYSNICRLFRF DGQEMYVK KLRPYDKTI+
Sbjct: 1801 REQHVKKVPHIRDAVWNSLRLTNSYTELHYYSNICRLFRFNDGQEMYVKLKLRPYDKTID 1860

Query: 1920 EDSGKVEPVGILPPETGAMPRADDDKRPLLFLAEDFQSRVNSPGGIRYVFQLQIRPVSHG 1979
            EDSGKVEP+GILPPETGA+PRADDDKRPLLFLAEDF +RVNSP G+RYVFQ+Q RPV   
Sbjct: 1861 EDSGKVEPIGILPPETGAIPRADDDKRPLLFLAEDFLNRVNSPEGVRYVFQIQTRPVPQD 1920

Query: 1980 EVDRDIALDCTKPWDEAEFPCLDIGEIKIDQSLSKEESEALEFNPFLRCPEVDVISATSA 2039
            E +RDIALDCTKPWDE EFPC DIGEI+I QSLSKEESEALEFNPFLRC EVDVISATS 
Sbjct: 1921 EAERDIALDCTKPWDETEFPCQDIGEIEIHQSLSKEESEALEFNPFLRCHEVDVISATSI 1980

Query: 2040 SQSASIDHGRSLIYEICQHLRNGSPLPEAWKIFLEQSDTKVDLSGCPVAAAVKRRDNEKA 2099
            SQSASIDHGRSLIYEICQHLRNG+PLPEAWKIFL+QSDTKVDLSGCP+AAA+K R NEK 
Sbjct: 1981 SQSASIDHGRSLIYEICQHLRNGTPLPEAWKIFLQQSDTKVDLSGCPMAAALKERANEKT 2040

Query: 2100 ALNRTWFQNFWVTFSQPLLQTALPYFIIGLVIFFPLAYVMHLKEDKKLPLQWLLPLLWVS 2159
            AL+RTW+QN W+TF QP  QTALPYFI+GLVIF PLA VMHLKE+KKLPL WLLPLLWVS
Sbjct: 2041 ALDRTWYQNLWLTFFQPTFQTALPYFIMGLVIFPPLASVMHLKENKKLPLHWLLPLLWVS 2100

Query: 2160 SGIIAALCCVLAKWVLVQKKKDGESIGIWSIRIFMDTIWQAIRTLVGDYFMEMTTGSFIF 2219
            SGIIAALCC++AKW+LVQKKK+GE+IGIWSIRIFMDT WQAI+T+VGDYFMEMTTGSFIF
Sbjct: 2101 SGIIAALCCIVAKWILVQKKKEGETIGIWSIRIFMDTTWQAIKTVVGDYFMEMTTGSFIF 2160

Query: 2220 VLIMKLMGSDVDLDQGTYVDSMGALLNPEMVKIHKGGSVGREALLFGHIYEGGGEVKFGK 2279
            VLIMKLMGSDVD+DQGTYVDSMGALLNPEMVKIH+GGSVGREALLFGHIYEGGGEVKFG 
Sbjct: 2161 VLIMKLMGSDVDMDQGTYVDSMGALLNPEMVKIHRGGSVGREALLFGHIYEGGGEVKFGN 2220

Query: 2280 IEIGEGGSVGSRAIAMPGVRVESEASLASLSLAMKEEIVRAS 2318
            IEIGE G VGSRAIAMPGVRVESEAS+A LSLAMKEEI+RA+
Sbjct: 2221 IEIGEDGFVGSRAIAMPGVRVESEASIAPLSLAMKEEIIRAT 2262

BLAST of MS010231 vs. TAIR 10
Match: AT1G20480.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 105.9 bits (263), Expect = 4.4e-22
Identity = 105/396 (26.52%), Postives = 179/396 (45.20%), Query Frame = 0

Query: 674  DDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKSTSRTVLVSWLPQYHDMGLIGG 733
            DD + L ++SG+TG +KGVM++H  LI  V+  R R+    RT+    +P  H  G  GG
Sbjct: 213  DDTAALLYSSGTTGTSKGVMLSHRNLIALVQAYRARFGLEQRTICT--IPMCHIFG-FGG 272

Query: 734  LFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATH-SAGPNFAFELVARRLEANKDKV 793
              T L++ G  I+  P   +      L  + T+++++ S  P     +V    E N    
Sbjct: 273  FATGLIALGWTIVVLPKFDMAK---LLSAVETHRSSYLSLVPPIVVAMVNGANEIN---- 332

Query: 794  QAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAENCVFVSCAFGEG 853
              Y+LSSL  ++    P+ +   ++F+E        +  +  GYGL E+    +  F + 
Sbjct: 333  SKYDLSSLHTVVAGGAPLSREVTEKFVE-----NYPKVKILQGYGLTESTAIAASMFNKE 392

Query: 854  KPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGRE 913
            +     + G +          +++ + V+P TG+ L  + + GE+WI SP+   GY+  +
Sbjct: 393  ETKRYGASGLLA--------PNVEGKIVDPDTGRVLGVN-QTGELWIRSPTVMKGYFKNK 452

Query: 914  ELSQDTFRNELQNRPGRRYIRTGDLGRII-DGKLFITGRIKDLIIAAGRNIYPADVEKTV 973
            E +  T  +E        +++TGDL  I  DG +F+  R+K+LI   G  + PA++E   
Sbjct: 453  EATASTIDSE-------GWLKTGDLCYIDGDGFVFVVDRLKELIKCNGYQVAPAELE--- 512

Query: 974  ESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEVKDGKPVAKDVIDQIQN 1033
                        A++    +I     IPIPD       +     K G  +++    +I  
Sbjct: 513  ------------ALLLAHPEIADAAVIPIPDMKAGQYPMAYIVRKVGSNLSE---SEIMG 559

Query: 1034 RVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLK 1068
             VA++        K+    +I K  SGKI R E  K
Sbjct: 573  FVAKQVSPYKKIRKVTFLASIPKNPSGKILRRELTK 559

BLAST of MS010231 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-19
Identity = 105/409 (25.67%), Postives = 179/409 (43.77%), Query Frame = 0

Query: 662  PEVVANQSEPHPDDVSFLQFTSGSTGDAKGVMITHGGLI-HNVKLMRRRYKSTSRTVLVS 721
            P   A +++ H DD + L ++SG+TG +KGV  +HG LI H  + +   ++   +T + +
Sbjct: 187  PSGQAVRNQVHKDDTAMLLYSSGTTGRSKGVNSSHGNLIAHVARYIAEPFEQPQQTFICT 246

Query: 722  WLPQYHDMGLIGGLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFEL 781
             +P +H  GL+  +   L  G T ++       +     +  +  Y+AT          +
Sbjct: 247  -VPLFHTFGLLNFVLATLALGTTVVILPRFDLGE----MMAAVEKYRATTLI---LVPPV 306

Query: 782  VARRLEANKDKVQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAE 841
            +   +      ++ Y++S L  +     P+ K   + F++    + + +     GY L E
Sbjct: 307  LVTMINKADQIMKKYDVSFLRTVRCGGAPLSKEVTQGFMKKYPTVDVYQ-----GYALTE 366

Query: 842  NCVFVSCAFGEGKPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWIS 901
                   + G G  I    + R   G V   +  ++ R V+P TG+ +  + + GE+W+ 
Sbjct: 367  -------SNGAGASIESVEESR-RYGAVGLLSCGVEARIVDPNTGQVMGLN-QTGELWLK 426

Query: 902  SPSAGIGYWGREELSQDTFRNELQNRPGRRYIRTGDLGRI-IDGKLFITGRIKDLIIAAG 961
             PS   GY          FRNE +      +++TGDL  I  DG LFI  R+K+LI   G
Sbjct: 427  GPSIAKGY----------FRNEEEIITSEGWLKTGDLCYIDNDGFLFIVDRLKELIKYKG 486

Query: 962  RNIYPADVEKTVESSSELLRPGCCAVIGVPEDILMEKGIPIPDC-SDQVGVVVIAEVKDG 1021
              + PA++E               A++    DIL    IP PD  + Q  +  +A   + 
Sbjct: 487  YQVPPAELE---------------ALLLNHPDILDAAVIPFPDKEAGQFPMAYVARKPES 544

Query: 1022 KPVAKDVIDQIQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLK 1068
                K VID I  +VA    +     K+    +I KT SGK  R + +K
Sbjct: 547  NLCEKKVIDFISKQVAPYKKIR----KVAFIDSIPKTPSGKTLRKDLIK 544

BLAST of MS010231 vs. TAIR 10
Match: AT1G20510.1 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 96.3 bits (238), Expect = 3.5e-19
Identity = 112/399 (28.07%), Postives = 181/399 (45.36%), Query Frame = 0

Query: 674  DDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKS-TSRTVLVSWLPQYHDMGLIG 733
            DD + L ++SG+TG +KGV+ +H  LI  V+ +  R+ S       +  +P +H  GL  
Sbjct: 189  DDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAA 248

Query: 734  GLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFELVARRLEANKDKV 793
                 L  G T I+ S     +     +  I  YQAT  + P     LVA    A++ K 
Sbjct: 249  FATGLLAYGSTIIVLSKFEMHE----MMSAIGKYQAT--SLPLVPPILVAMVNGADQIKA 308

Query: 794  QAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAENCVFVSCAFGEG 853
            + Y+LSS+  ++    P+ K   + F E    + + +     GYGL E     S   G  
Sbjct: 309  K-YDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQ-----GYGLTE-----STGIGAS 368

Query: 854  KPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGRE 913
                 +S+     G +   +A ++ R V+P TG+ L    + GE+W+  PS   GY+  E
Sbjct: 369  TDTVEESRRYGTAGKL---SASMEGRIVDPVTGQILGPK-QTGELWLKGPSIMKGYFSNE 428

Query: 914  ELSQDTFRNELQNRPGRRYIRTGDLGRI-IDGKLFITGRIKDLIIAAGRNIYPADVEKTV 973
            E +  T  +E        ++RTGDL  I  DG +F+  R+K+LI   G  + PA++E   
Sbjct: 429  EATSSTLDSE-------GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELE--- 488

Query: 974  ESSSELLRPGCCAVIGVPEDILMEKGIPIPDCSDQVGVVVIAEV--KDGKPVA-KDVIDQ 1033
                        A++    +I     IP PD   +VG   +A V  K G  ++ K +++ 
Sbjct: 489  ------------ALLLTHPEITDAAVIPFPD--KEVGQFPMAYVVRKTGSSLSEKTIMEF 538

Query: 1034 IQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLK 1068
            +  +VA    +     K+    +I K  SGKI R + +K
Sbjct: 549  VAKQVAPYKRIR----KVAFVSSIPKNPSGKILRKDLIK 538

BLAST of MS010231 vs. TAIR 10
Match: AT1G20510.2 (OPC-8:0 CoA ligase1 )

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-18
Identity = 89/306 (29.08%), Postives = 144/306 (47.06%), Query Frame = 0

Query: 674 DDVSFLQFTSGSTGDAKGVMITHGGLIHNVKLMRRRYKS-TSRTVLVSWLPQYHDMGLIG 733
           DD + L ++SG+TG +KGV+ +H  LI  V+ +  R+ S       +  +P +H  GL  
Sbjct: 189 DDTATLLYSSGTTGMSKGVISSHRNLIAMVQTIVNRFGSDDGEQRFICTVPMFHIYGLAA 248

Query: 734 GLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSAGPNFAFELVARRLEANKDKV 793
                L  G T I+ S     +     +  I  YQAT  + P     LVA    A++ K 
Sbjct: 249 FATGLLAYGSTIIVLSKFEMHE----MMSAIGKYQAT--SLPLVPPILVAMVNGADQIKA 308

Query: 794 QAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAENCVFVSCAFGEG 853
           + Y+LSS+  ++    P+ K   + F E    + + +     GYGL E     S   G  
Sbjct: 309 K-YDLSSMHTVLCGGAPLSKEVTEGFAEKYPTVKILQ-----GYGLTE-----STGIGAS 368

Query: 854 KPIFIDSQGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWGRE 913
                +S+     G +   +A ++ R V+P TG+ L    + GE+W+  PS   GY+  E
Sbjct: 369 TDTVEESRRYGTAGKL---SASMEGRIVDPVTGQILGPK-QTGELWLKGPSIMKGYFSNE 428

Query: 914 ELSQDTFRNELQNRPGRRYIRTGDLGRI-IDGKLFITGRIKDLIIAAGRNIYPADVEKTV 973
           E +  T  +E        ++RTGDL  I  DG +F+  R+K+LI   G  + PA++E  +
Sbjct: 429 EATSSTLDSE-------GWLRTGDLCYIDEDGFIFVVDRLKELIKYKGYQVAPAELEALL 466

Query: 974 ESSSEL 978
            +  E+
Sbjct: 489 LTHPEI 466

BLAST of MS010231 vs. TAIR 10
Match: AT1G20500.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.9e-18
Identity = 104/399 (26.07%), Postives = 174/399 (43.61%), Query Frame = 0

Query: 674  DDVSFLQFTSGSTGDAKGVMITHGGL-IHNVKLMRRRYKSTSRTVLVSWLPQYHDMGLIG 733
            DD + + ++SG+TG +KGV+ +H  L  H  + +    K     + +  +P +H  GL+ 
Sbjct: 197  DDTAMMLYSSGTTGPSKGVISSHRNLTAHVARFISDNLKRDD--IFICTVPMFHTYGLLT 256

Query: 734  GLFTALVSGGTAILFSPMTFIKNPLLWLQTISTYQATHSA-GPNFAFELVARRLEANKDK 793
                 +  G T ++             +  +  ++AT  A  P     LVA   +A+  K
Sbjct: 257  FAMGTVALGSTVVILRRFQLHD----MMDAVEKHRATALALAPPV---LVAMINDADLIK 316

Query: 794  VQAYNLSSLVFLMVAAEPVRKITLKRFIELTIPLGLSEEVMAPGYGLAENCVFVSCAFGE 853
             + Y+LSSL  +     P+ K   + F+E    + + +     GY L E+          
Sbjct: 317  AK-YDLSSLKTVRCGGAPLSKEVTEGFLEKYPTVDILQ-----GYALTES---------N 376

Query: 854  GKPIFIDS-QGRVCCGYVDQDNADIDIRTVNPGTGKELEEDGKEGEIWISSPSAGIGYWG 913
            G   F +S +     G      +D++ R V+P TG+ +  + + GE+W+  PS   GY+ 
Sbjct: 377  GGGAFTNSAEESRRYGTAGTLTSDVEARIVDPNTGRFMGIN-QTGELWLKGPSISKGYFK 436

Query: 914  REELSQDTFRNELQNRPGRRYIRTGDLGRI-IDGKLFITGRIKDLIIAAGRNIYPADVEK 973
             +E + +T   E        +++TGDL  I  DG LF+  R+K+LI   G  + PA++E 
Sbjct: 437  NQEATNETINLE-------GWLKTGDLCYIDEDGFLFVVDRLKELIKYKGYQVPPAELE- 496

Query: 974  TVESSSELLRPGCCAVIGVPEDILMEKGIPIPDC-SDQVGVVVIAEVKDGKPVAKDVIDQ 1033
                          A++    DIL    IP PD  + Q  +  +    +     K VID 
Sbjct: 497  --------------ALLITHPDILDAAVIPFPDKEAGQYPMAYVVRKHESNLSEKQVIDF 544

Query: 1034 IQNRVAEEHGVSVASVKLIKPRTISKTTSGKIKRFECLK 1068
            I  +VA      +  V  I   +I KT SGK  R + +K
Sbjct: 557  ISKQVAPYK--KIRKVSFI--NSIPKTASGKTLRKDLIK 544