MS010225 (gene) Bitter gourd (TR) v1

BLAST of MS010225 vs. NCBI nr
Match: XP_022153766.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1839.7 bits (4764), Expect = 0.0e+00
Identity = 894/898 (99.55%), Postives = 896/898 (99.78%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR 60
           MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR
Sbjct: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR 60

Query: 61  REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT 120
           REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT
Sbjct: 61  REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT 120

Query: 121 GSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTGLA 180
           GSEKATIRVEAKLTSEDLVEEIYEASF+VASDFGEIGAVIVENPNEKEMYIKEVDLTGLA
Sbjct: 121 GSEKATIRVEAKLTSEDLVEEIYEASFQVASDFGEIGAVIVENPNEKEMYIKEVDLTGLA 180

Query: 181 SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK 240
           SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK
Sbjct: 181 SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK 240

Query: 241 STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY 300
           STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY
Sbjct: 241 STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY 300

Query: 301 VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP 360
           VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP
Sbjct: 301 VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP 360

Query: 361 TSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQSVT 420
           TSLNDLLPW FKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQ VT
Sbjct: 361 TSLNDLLPWRFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQLVT 420

Query: 421 EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR 480
           EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR
Sbjct: 421 EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR 480

Query: 481 ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK 540
           ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK
Sbjct: 481 ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK 540

Query: 541 AHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR 600
           AHALVNDAGYHQL+THWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR
Sbjct: 541 AHALVNDAGYHQLITHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR 600

Query: 601 SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK 660
           SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK
Sbjct: 601 SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK 660

Query: 661 LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY 720
           LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY
Sbjct: 661 LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY 720

Query: 721 APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP 780
           APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP
Sbjct: 721 APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP 780

Query: 781 ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA 840
           ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA
Sbjct: 781 ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA 840

Query: 841 AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
           AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI
Sbjct: 841 AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 898

BLAST of MS010225 vs. NCBI nr
Match: XP_022153765.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1570.4 bits (4065), Expect = 0.0e+00
Identity = 771/906 (85.10%), Postives = 821/906 (90.62%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSS----NGRLS 60
           MLKGQGFG   RAAAPASLRH+QRP L SI+A AAF+P Y N   QKQKSS    NGRL 
Sbjct: 1   MLKGQGFGCSPRAAAPASLRHLQRPLLSSINAGAAFMPHYSNSRWQKQKSSKSSRNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LVC REIKA L+T+A+PTT+VQTKVVVKKITG  TSS  LRD K DVEKFLKL+FASN L
Sbjct: 61  LVC-REIKAALATAAEPTTTVQTKVVVKKITGEITSSTLLRDAK-DVEKFLKLEFASNQL 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           DPRTGSEK TIRVEAKL SEDLVEEIYEASFEV  DFGEIGAVIVENPNEKEMYIKEVDL
Sbjct: 121 DPRTGSEKPTIRVEAKLASEDLVEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
           TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT
Sbjct: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPV 300
           GERK+TD IYDYDVYNDLGDCT EEL+RPVLGG ERPYPRRCRTGRPHC +DPD+EE+  
Sbjct: 241 GERKATDRIYDYDVYNDLGDCTSEELKRPVLGGPERPYPRRCRTGRPHCTEDPDSEERTT 300

Query: 301 GSMYVPRDQTFSNVKQAVFTID----LLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGF 360
           G++YVPRD+TFS +KQ  F +D    +LR   P+L++ FD+   F NF+ IDAL+  DGF
Sbjct: 301 GAIYVPRDETFSTIKQGAFNVDTILSVLRTILPRLQVHFDRDTGFKNFKSIDALYDVDGF 360

Query: 361 NLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLN 420
           NL  LES  SL DLLPWIFKLVSET EFFL FQ PEPMDRDKFFWLSDEEFAR+TLAGLN
Sbjct: 361 NLPPLESTDSLKDLLPWIFKLVSETGEFFLRFQSPEPMDRDKFFWLSDEEFARQTLAGLN 420

Query: 421 PYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDML 480
           P SIQ VTEWPL+SKLDPEIYGPPESAFNT+ML+QQIGSMTVQEAIQKKKL+I+DYHDML
Sbjct: 421 PCSIQLVTEWPLVSKLDPEIYGPPESAFNTEMLNQQIGSMTVQEAIQKKKLYILDYHDML 480

Query: 481 LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH 540
           LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH
Sbjct: 481 LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH 540

Query: 541 LWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYT 600
           LW+WRFAKAH L +DAGYHQLV+HW+RTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRYT
Sbjct: 541 LWIWRFAKAHVLAHDAGYHQLVSHWVRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYT 600

Query: 601 MEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEED 660
           +EINA ARSNLINA G IETTFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEED
Sbjct: 601 LEINAAARSNLINAGGTIETTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEED 660

Query: 661 PFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRT 720
           P ASHGL+LNIKDYPFANDGLILWDALK+WVTEYVNHYYPDPYLITSDTELQAWWTEIRT
Sbjct: 661 PTASHGLRLNIKDYPFANDGLILWDALKQWVTEYVNHYYPDPYLITSDTELQAWWTEIRT 720

Query: 721 VGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN 780
           VGHADKK A GWP LNTPQDLI+I++NIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN
Sbjct: 721 VGHADKKDAAGWPTLNTPQDLIEIVSNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN 780

Query: 781 MPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSW 840
           MPTED NPALW+SFLEKPEDVLLDAFPS  QAT VM+VLDVLSSHSPDEEYIGK MEPSW
Sbjct: 781 MPTEDPNPALWKSFLEKPEDVLLDAFPSQYQATQVMMVLDVLSSHSPDEEYIGKNMEPSW 840

Query: 841 GDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGV 899
           GDDPVIKAAFD+F+ KMKELE+I+D RNAD +LKNRTGAG+TPYELLKPFSGPGVTGKGV
Sbjct: 841 GDDPVIKAAFDKFNAKMKELELIVDLRNADLDLKNRTGAGVTPYELLKPFSGPGVTGKGV 900

BLAST of MS010225 vs. NCBI nr
Match: XP_022148710.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 722/907 (79.60%), Postives = 789/907 (86.99%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSS----NGRLS 60
           MLKGQGFG  T AAAPASL H+QRP LCS +A AAFLP Y  PSCQKQ+SS    NGRL 
Sbjct: 1   MLKGQGFGCSTGAAAPASLSHLQRPLLCSTNAGAAFLPHYSKPSCQKQRSSKSSRNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LVC REIKA ++T+A+PTT+VQTKVVV+K+TG  TSS  +RDVK DVEKFLKL+FASNLL
Sbjct: 61  LVC-REIKAAIATAAEPTTTVQTKVVVQKVTGEITSSTIVRDVKPDVEKFLKLEFASNLL 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           D +TGSEKA I+VEA+L+SED  EEIYEASFEV  DFGEIGAVIVENPNEKEMYIKEVDL
Sbjct: 121 DLKTGSEKARIKVEARLSSEDPKEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
           TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLP  TPAGLKSMRA ELVNLRGNGT
Sbjct: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPV 300
           GER   D IYDYDVYNDLG C+  +L+RPVLGG E PYPRRCRTGRPHC+  P+ EE+  
Sbjct: 241 GERVPYDRIYDYDVYNDLGSCS-GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRK 300

Query: 301 G-SMYVPRDQTFSNVKQAVF----TIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDG 360
             S+Y+PRD+ FSN+KQ  F     +  +    P L + FD KA FSNF++IDAL++ DG
Sbjct: 301 NESIYIPRDEAFSNIKQDAFMKDSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDG 360

Query: 361 FNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGL 420
           F L  LES TSL D LPWIFKL S T +  L F  PEPMDRDKFFWLSDE+FAR+TLAGL
Sbjct: 361 FKLPTLESSTSLKDDLPWIFKLDS-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGL 420

Query: 421 NPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDM 480
           NP SIQ VTEWPL+SKLDPEIYGPPESAFN +ML+Q+IGS+TVQEAIQKKKL+I+DYHDM
Sbjct: 421 NPCSIQLVTEWPLVSKLDPEIYGPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDM 480

Query: 481 LLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQAT 540
           LLPYVQKVRA++GTTLYGSRTLFFLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQAT
Sbjct: 481 LLPYVQKVRAVEGTTLYGSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQAT 540

Query: 541 HLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRY 600
           HLW+WRFAKAH L +DAGYHQLV+HWLRTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRY
Sbjct: 541 HLWIWRFAKAHVLAHDAGYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRY 600

Query: 601 TMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEE 660
           T+EINA AR+NLI+A G IE TFS +KYSMELSSTAY LQWQFDLQALPADLIHRGLAEE
Sbjct: 601 TLEINASARTNLISAGGTIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEE 660

Query: 661 DPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIR 720
           DP ASHGLKLNIKDYPFANDGLILWDALKEWVTEYVN+YY D  ++TSD ELQ WWTEIR
Sbjct: 661 DPTASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIR 720

Query: 721 TVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIART 780
           TVGHADKK A GWP LNTPQDLIDI+TNIAWVAS HHAAVNFGQYAYAGYFPNRPSI +T
Sbjct: 721 TVGHADKKDAAGWPTLNTPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKT 780

Query: 781 NMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPS 840
           NMPTED NPALW+ FLEKPE  LLDAFPS  QAT VM+VL++LSSHSPDEEY+GK MEPS
Sbjct: 781 NMPTEDPNPALWKCFLEKPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPS 840

Query: 841 WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKG 899
           WGDDPVIKAAFDRFS KMK LEVIID RNAD +LKNRTGAG++PYELLKPFS  GVTGKG
Sbjct: 841 WGDDPVIKAAFDRFSAKMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKG 900

BLAST of MS010225 vs. NCBI nr
Match: XP_022995022.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 689/907 (75.96%), Postives = 777/907 (85.67%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSL-CSIHAEAAFLPPYWNPSCQKQKS---SNGRLS 60
           MLK QG  GC+   APA+L H+QRP L   I+A  +F  P+WN    KQK+   SNGRL 
Sbjct: 1   MLKSQGV-GCSTRVAPATLCHLQRPLLGRGINAGVSF--PFWNNPGNKQKAKSISNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LV  REIKA L+T A+PTT+V TKV+VKKITG  TSS  L + K  ++K LKL+FAS L+
Sbjct: 61  LV-SREIKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESK-ALDKLLKLEFASILV 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           DPRTG EK  I V+AKL SED++EEIYEAS EV +DFG+IGAVIVEN NEKEM++KEV+L
Sbjct: 121 DPRTGLEKPPISVQAKLISEDVLEEIYEASLEVPADFGDIGAVIVENHNEKEMFVKEVEL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
            GLASGP  +TCNSWVQP T++P   RVFFT KSYLP QTPAGL+SMR  ELVNLRGNGT
Sbjct: 181 NGLASGPLIVTCNSWVQPKTLVPTQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKP 300
           GER+S D +YDYDVYNDLGD    E+L+RPVLGG ERPYPRRCRTGRP  K DP++E++ 
Sbjct: 241 GERRSYDRVYDYDVYNDLGDPDKSEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRA 300

Query: 301 VGSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDG 360
            GS+YVPRD+TFS++KQ VF     + +LRA  P+L++ FD+   F NF+ +DALF  DG
Sbjct: 301 TGSIYVPRDETFSDLKQGVFNASTLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDG 360

Query: 361 FNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGL 420
           FNL  LES TSL DLLPWIFK +SET +F   FQ PEPMDRDKFFWL DEEFAR+TLAGL
Sbjct: 361 FNLPPLESTTSLKDLLPWIFKFLSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGL 420

Query: 421 NPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDM 480
           NP SIQ V EWPLMS+L+PEIYGPPESAFNTQM+D++IGSMTVQEAIQ+KKLFI+DY+DM
Sbjct: 421 NPCSIQLVKEWPLMSQLNPEIYGPPESAFNTQMIDEEIGSMTVQEAIQEKKLFILDYNDM 480

Query: 481 LLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQAT 540
           LLPYV++VRALKGTTLYGSRTLFFLNED TLRPLAIELTRPPMD KPQWK VYGPSE AT
Sbjct: 481 LLPYVRRVRALKGTTLYGSRTLFFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENAT 540

Query: 541 HLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRY 600
           +LW+WRFAK+H L +DAGYHQLV+HWLRTHC+VEPY IATNRQLSAMHPI+RLL+PHFRY
Sbjct: 541 NLWLWRFAKSHVLAHDAGYHQLVSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRY 600

Query: 601 TMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEE 660
           TMEINA AR+NLINA G IE+TFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEE
Sbjct: 601 TMEINAAARTNLINAGGTIESTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEE 660

Query: 661 DPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIR 720
           DP ASHGLKL+IKDYPFANDGLILWDALK+WVTEYVNHYYPDP L+ SDTELQAWWTEIR
Sbjct: 661 DPTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNHYYPDPSLVASDTELQAWWTEIR 720

Query: 721 TVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIART 780
           TVGHADK+  P WP LNTPQDLIDI+TNIAWVASAHHAAVNFGQYAY GYFP+RPSIART
Sbjct: 721 TVGHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYGGYFPSRPSIART 780

Query: 781 NMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPS 840
           NMPTED NPALW+SFLEKPE+VLL+AFPS  QAT VMLVLDVLSSHSPDEEY+GK MEPS
Sbjct: 781 NMPTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPS 840

Query: 841 WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKG 899
           WGD+P IKAAFDRF+ +MKELE+IID+RNAD NLKNRTGAG+TPYELLKPFSGPGVTGKG
Sbjct: 841 WGDNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKG 900

BLAST of MS010225 vs. NCBI nr
Match: KAG6605891.1 (Linoleate 13S-lipoxygenase 2-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1392.1 bits (3602), Expect = 0.0e+00
Identity = 687/906 (75.83%), Postives = 778/906 (85.87%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKS---SNGRLSL 60
           MLK QG  GC    APA+L H+QRP    I+A  +F  P+WN +  KQK+   SNGRL L
Sbjct: 1   MLKSQGV-GCATRVAPATLCHLQRPLPRGINAGVSF--PFWNNTQNKQKAKSISNGRLRL 60

Query: 61  VCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLD 120
           V  REIKA L+T A+PTT+V TKV+VKKITG  TSS  L + K  ++K LKL+FAS L+D
Sbjct: 61  V-SREIKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESK-ALDKLLKLEFASILVD 120

Query: 121 PRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLT 180
           P+TG EK  I V+AKL SED++EEIYEAS EV++DFG+IGAVIVEN NEKEM++KEV+L 
Sbjct: 121 PKTGLEKPPISVQAKLISEDVLEEIYEASLEVSADFGDIGAVIVENHNEKEMFVKEVELN 180

Query: 181 GLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTG 240
           GLASGP  +TCNSWVQP T++P   RVFFT KSYLP QTPAGL+SMR  ELVNLRGNGTG
Sbjct: 181 GLASGPLIVTCNSWVQPKTLVPTQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGTG 240

Query: 241 ERKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPV 300
           ER+S D IYDYDVYNDLGD    E+L+RPVLGG ERPYPRRCRTGRP  K DP++E++  
Sbjct: 241 ERQSYDRIYDYDVYNDLGDPDKSEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRAT 300

Query: 301 GSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGF 360
           GS+YVPRD+TFS++KQ VF     + +LRA  P+L++ F++   F NF+ +DALF  DGF
Sbjct: 301 GSIYVPRDETFSDLKQGVFNASTLLSVLRAIIPRLQVHFNQNTGFPNFKAVDALFDVDGF 360

Query: 361 NLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLN 420
           NL  LES TSL DLLPWIFK +SET +F   FQ PEPMDRDKFFWL DEEFAR+TLAGLN
Sbjct: 361 NLPPLESTTSLKDLLPWIFKFLSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGLN 420

Query: 421 PYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDML 480
           P SI+ V EWPLMS+L+PEIYGPPESAFNTQM+D++IGSMTVQEAIQ+KKLFI+DY+DML
Sbjct: 421 PCSIRLVKEWPLMSQLNPEIYGPPESAFNTQMIDREIGSMTVQEAIQQKKLFILDYNDML 480

Query: 481 LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH 540
           LPYV++VRALKGTTLYGSRTLFFLNED TLRPLAIELTRPPMD KPQWK VYGPSE AT+
Sbjct: 481 LPYVRRVRALKGTTLYGSRTLFFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENATN 540

Query: 541 LWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYT 600
           LW+WRFAK+H L +DAGYHQLV+HWLRTHC+VEPY IATNRQLSAMHPI+RLL+PHFRYT
Sbjct: 541 LWLWRFAKSHVLAHDAGYHQLVSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRYT 600

Query: 601 MEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEED 660
           MEINA AR+NLINA G IE+TFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEED
Sbjct: 601 MEINAAARTNLINAGGTIESTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEED 660

Query: 661 PFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRT 720
           P ASHGLKL+IKDYPFANDGLILWDALK+WVTEYVNHYYPDP L+ SDTELQAWWTEIRT
Sbjct: 661 PTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNHYYPDPSLVASDTELQAWWTEIRT 720

Query: 721 VGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN 780
           VGHADK+  P WP LNTPQDLIDI+TNIAWVASAHHAAVNFGQYAYAGYFP+RPSIARTN
Sbjct: 721 VGHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIARTN 780

Query: 781 MPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSW 840
           MPTED NPALW+SFLEKPE+VLL+AFPS  QAT VMLVLDVLSSHSPDEEY+GK MEPSW
Sbjct: 781 MPTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPSW 840

Query: 841 GDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGV 899
           GD+P IKAAFDRF+ +MKELE+IID+RNAD NLKNRTGAG+TPYELLKPFSGPGVTGKGV
Sbjct: 841 GDNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKGV 900

BLAST of MS010225 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 976.1 bits (2522), Expect = 2.7e-283
Identity = 494/845 (58.46%), Postives = 611/845 (72.31%), Query Frame = 0

Query: 64  KAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRTGSE 123
           KA LS++ K  T V+  V V+K   L+  S+ L D+ D + K L L   +  LD +TG E
Sbjct: 60  KAVLSSTEK-ATGVKAVVTVQKQVNLNL-SRGLDDIGDLLGKSLLLWIVAAELDHKTGIE 119

Query: 124 KATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTGLASGP 183
           K  IR  A    +   +  YEA F +  DFGE+GA+++EN + KEMY+K + + G   G 
Sbjct: 120 KPGIRAYAHRGRDVDGDTHYEADFVIPQDFGEVGAILIENEHHKEMYVKNIVIDGFVHGK 179

Query: 184 STITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKSTD 243
             ITCNSWV      P  +R+FFT+KSYLP QTP+G+  +R  ELV LRG+G GERK  +
Sbjct: 180 VEITCNSWVHSKFDNPD-KRIFFTNKSYLPSQTPSGVSRLREEELVTLRGDGIGERKVFE 239

Query: 244 NIYDYDVYNDLG--DCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMYV 303
            IYDYDVYNDLG  D   ++ +RPVLGG E PYPRRC+TGRP  KKDP +E +    +YV
Sbjct: 240 RIYDYDVYNDLGEADSNNDDAKRPVLGGKELPYPRRCKTGRPRSKKDPLSETRST-FVYV 299

Query: 304 PRDQTFSNVKQAVFT----IDLLRAAFPKLE-IFFDKKATFSNFEEIDALFSRDGFNLRN 363
           PRD+ FS VK   F+      +L A  P LE +  D    F +F  ID+LF+  G +L  
Sbjct: 300 PRDEAFSEVKSVAFSGNTVYSVLHAVVPALESVVTDPNLGFPHFPAIDSLFN-VGVDLPG 359

Query: 364 L-ESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYS 423
           L +  + L +++P + K +S+T +  L F+ P+ + RDKF W  D EFAR+TLAGLNPYS
Sbjct: 360 LGDKKSGLFNVVPRLIKAISDTRKDVLLFESPQLVQRDKFSWFRDVEFARQTLAGLNPYS 419

Query: 424 IQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGS-MTVQEAIQKKKLFIVDYHDMLLP 483
           I+ VTEWPL SKLDP++YGPPES    ++++++IG+ MTV++A+Q+KKLFI+DYHD+LLP
Sbjct: 420 IRLVTEWPLRSKLDPKVYGPPESEITKELIEKEIGNYMTVEQAVQQKKLFILDYHDLLLP 479

Query: 484 YVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSE-QATHL 543
           YV KV  LKG+ LYGSRT+FFL    TL+PLAIELTRPP+DDKPQWK VY P++  AT  
Sbjct: 480 YVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAIELTRPPVDDKPQWKEVYSPNDWNATGA 539

Query: 544 WVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTM 603
           W+W+ AKAH L +D+GYHQLV+HWLRTHC  EPY IA+NRQLSAMHPIYRLLHPHFRYTM
Sbjct: 540 WLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEPYIIASNRQLSAMHPIYRLLHPHFRYTM 599

Query: 604 EINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDP 663
           EINA AR  LINA+G+IE++F   KY++ELSS AY  +W+FD +ALP +LI RGLA EDP
Sbjct: 600 EINALAREALINANGVIESSFFPGKYAIELSSIAYGAEWRFDQEALPQNLISRGLAVEDP 659

Query: 664 FASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTV 723
              HGLKL I+DYPFANDGL+LWD LK+WVT YVNHYYP   LI SD ELQAWW+EI+ V
Sbjct: 660 NEPHGLKLAIEDYPFANDGLVLWDILKQWVTNYVNHYYPQTNLIESDKELQAWWSEIKNV 719

Query: 724 GHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNM 783
           GH DK+  P WP L TP DLI IIT I WV S HHAAVNFGQY+YAGYFPNRP++AR+ M
Sbjct: 720 GHGDKRDEPWWPELKTPNDLIGIITTIVWVTSGHHAAVNFGQYSYAGYFPNRPTVARSKM 779

Query: 784 PTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWG 843
           PTED     W  F+ KPE+ LL  FPS  QAT VM +LDVLS+HSPDEEYIG+ +EP W 
Sbjct: 780 PTEDPTAEEWEWFMNKPEEALLRCFPSQIQATKVMAILDVLSNHSPDEEYIGEKIEPYWA 839

Query: 844 DDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVP 899
           +DPVI AAF+ FS K+KELE IID RN D  L NR GAG+ PYELLKP+S PGVTGKGVP
Sbjct: 840 EDPVINAAFEVFSGKLKELEGIIDARNNDSKLSNRNGAGVMPYELLKPYSEPGVTGKGVP 899

BLAST of MS010225 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 4.1e-263
Identity = 461/849 (54.30%), Postives = 585/849 (68.90%), Query Frame = 0

Query: 61  REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT 120
           + I+     + +PT S     V   + G    S     V D + +   L+  S+ LD  +
Sbjct: 66  KSIRKKCVVTVQPTISGALTAVTVGLLGTVADS-----VSDFLGRSFLLELVSSDLD-SS 125

Query: 121 GSEKATIRVEAKLTSEDLVEEI--YEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTG 180
           G EK T++  A     D   ++  Y+  FEV  DFGEIGAV+V+N   ++ Y+K + L  
Sbjct: 126 GKEKDTVKAYATYDELDKESKLYKYQCEFEVPDDFGEIGAVLVQNERHRDAYVKNIVLDE 185

Query: 181 LASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGE 240
           +     T TC+SW+      P  +R+FF +KSYLP +TP GLKS+R  +L +LRGNG GE
Sbjct: 186 IV----TFTCDSWIHSKFDNPD-KRIFFLNKSYLPSETPEGLKSLRQKDLESLRGNGEGE 245

Query: 241 RKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVG 300
           R+S D IYDYD YND+GD  T  ++ RPVLGG E P+PRRCRTGR     +P +E +   
Sbjct: 246 RQSFDRIYDYDTYNDIGDPDTDSDMARPVLGGNEHPFPRRCRTGRKMTSTEPWSESRTTL 305

Query: 301 SMYVPRDQTFSNVKQ----AVFTIDLLRAAFPKL-EIFFDKKATFSNFEEIDALFSRDGF 360
             YVPRD+ F+ +KQ    A     +L    P L  +  D+   F  F +I+ L+ +   
Sbjct: 306 PFYVPRDEDFAEIKQITRGATTLYSVLHGVIPALSSVLKDEDKGFPLFRDIELLYEKG-- 365

Query: 361 NLRNLESPTSLNDL--LPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAG 420
              +++ P     L  LP + K ++ + +  L F+ P    +D F W  DEEF R+TLAG
Sbjct: 366 --VDIDPPPDSGTLSALPRLVKAITNSTKKVLQFETPRIKHKDSFSWFRDEEFCRQTLAG 425

Query: 421 LNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQI-GSMTVQEAIQKKKLFIVDYH 480
           LNPYSIQ VTEWPLMSKLDPE+YGP ESA   + ++++I G MT +EA+++K+LF++DYH
Sbjct: 426 LNPYSIQLVTEWPLMSKLDPEVYGPAESAITKETVEEEIKGFMTXEEALEQKRLFLLDYH 485

Query: 481 DMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQ 540
           D+LLPYV KVR ++GTTLYGSRTL FL    TLRPLAIELTRPP + KPQWK VY P   
Sbjct: 486 DLLLPYVNKVREIEGTTLYGSRTLMFLTCTGTLRPLAIELTRPPNNGKPQWKHVYTPCWD 545

Query: 541 ATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHF 600
           AT  W+W+ AKAH L +D+GYHQLV+HWLRTHC  EPY IATNRQLS MHPI RLL PH 
Sbjct: 546 ATDXWLWKLAKAHVLAHDSGYHQLVSHWLRTHCVTEPYIIATNRQLSKMHPIQRLLCPHL 605

Query: 601 RYTMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLA 660
           RYTM+IN  AR +LINA+GIIE++FS +KYSM+LSS AY  +W+FD +ALPADLI RG+A
Sbjct: 606 RYTMQINGLARLSLINANGIIESSFSPRKYSMQLSSDAYAQKWRFDHEALPADLISRGMA 665

Query: 661 EEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTE 720
            ED  A HG+KL I+DYPFANDGL+LWDA+K+W T Y+NHYYP   L+ SD ELQAWWTE
Sbjct: 666 VEDESAPHGIKLTIEDYPFANDGLLLWDAIKQWATAYINHYYPQAKLVESDEELQAWWTE 725

Query: 721 IRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIA 780
           IRTVGHADKK  P WP L T QDLI +++ I WV+S HH+AVNFGQY + GYFPNRP+IA
Sbjct: 726 IRTVGHADKKDEPWWPQLKTQQDLIGVVSTIMWVSSGHHSAVNFGQYDFGGYFPNRPTIA 785

Query: 781 RTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTME 840
           RT MP ED     W +F+EKPEDVLL+ FP+  QAT VM +LDVLSSHSPDEEYIG +ME
Sbjct: 786 RTKMPNEDPTFEEWEAFMEKPEDVLLNCFPTQIQATKVMAILDVLSSHSPDEEYIGTSME 845

Query: 841 PSWGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTG 899
            SW  +P IK+AF+ F  ++K+L+ IID RN D  L+NRTGAG+  Y+LLKPFSG GVTG
Sbjct: 846 ASWEAEPAIKSAFEEFCGRLKKLDDIIDSRNRDPILRNRTGAGLVQYQLLKPFSGHGVTG 899

BLAST of MS010225 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 6.7e-258
Identity = 453/873 (51.89%), Postives = 580/873 (66.44%), Query Frame = 0

Query: 42  NPSCQKQKSSNGRLSLVCRREIKAGLSTSAKPTTSVQTKVVVKKITG-LSTSSQFLRDVK 101
           NP    ++++  R +L  R ++ A  +   +   +V+  +   K+ G ++   +FL  + 
Sbjct: 31  NPISAGRRNNLPRPNLRRRCKVTASRANIEQEGNTVKEPIQNIKVKGYITAQEEFLEGIT 90

Query: 102 -----DDVEKFLKLQFASNLLDPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGE 161
                DD+           L+  +T  ++ T+   A+    +  +E YE  FE+  DFG 
Sbjct: 91  WSRGLDDIADIRGRSLLVELISAKT-DQRITVEDYAQRVWAEAPDEKYECEFEMPEDFGP 150

Query: 162 IGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQ 221
           +GA+ ++N   +++++K V+L  L  G  T TC SWV P +V P  +R+FF+ KSYLP Q
Sbjct: 151 VGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDP-TKRIFFSDKSYLPSQ 210

Query: 222 TPAGLKSMRAAELVNLRGNG---TGERKSTDNIYDYDVYNDLGDCTLE-ELRRPVLGGLE 281
           TP  LK  R  EL  L+G      GE    + IYDYDVYND+GD   + EL RPV+GGL 
Sbjct: 211 TPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLT 270

Query: 282 RPYPRRCRTGRPHCKKDPDTEEKPVGSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKL 341
            PYPRRC+TGR  C+ DP +E++  G  YVPRD+ FS  K   FT    +  L + FP++
Sbjct: 271 HPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQI 330

Query: 342 E-IFFDKKATFSNFEEIDALFSRDGFNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQR 401
           E +    +  F +F+ I  LF  +G     L     L  LLP I K + E ++  L F  
Sbjct: 331 ESVLLSPQEPFPHFKAIQNLF-EEGI---QLPKDAGLLPLLPRIIKALGEAQDDILQFDA 390

Query: 402 PEPMDRDKFFWLSDEEFARETLAGLNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLD 461
           P  ++RD+F WL D+EFAR+TLAGLNPYSIQ V EWPL+SKLDP +YG P S    ++++
Sbjct: 391 PVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVE 450

Query: 462 QQI-GSMTVQEAIQKKKLFIVDYHDMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPL 521
           +++ G+MTV EA++ K+LF++DYHD+LLPYV KVR L  TTLY SRTLFFL++D TLRP+
Sbjct: 451 REVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPV 510

Query: 522 AIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVE 581
           AIELT PP  +KPQWK+V+ P   AT  W+W  AK HA+ +DAGYHQL++HWLRTH   E
Sbjct: 511 AIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTE 570

Query: 582 PYCIATNRQLSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTFSTKKYSMELSS 641
           PY IA NRQLSAMHPIYRLLHPHFRYTMEINA AR +L+N  GIIET F   KY++ELSS
Sbjct: 571 PYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSS 630

Query: 642 TAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTE 701
             Y   W+FD + LPADLI RGLAEED  A HG++L I DYPFANDGLILWDA+KEWVT+
Sbjct: 631 AVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTD 690

Query: 702 YVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVAS 761
           YV HYYPD  LITSD ELQ WW+E+R +GH DKK  P WP L T  DLI ++T IAWV S
Sbjct: 691 YVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTS 750

Query: 762 AHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQAT 821
            HHAAVNFGQY Y GYFPNRP+  R  MPTED      + F E PE VLL  +PS  QAT
Sbjct: 751 GHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQAT 810

Query: 822 TVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNL 881
            VM+ LD+LS+HSPDEEYIG+  E SW ++PVI AAF+RF  K++ LE +ID RN +  L
Sbjct: 811 LVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITL 870

Query: 882 KNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
           KNR GAG+  YELLKP S  GVTG GVP S+SI
Sbjct: 871 KNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

BLAST of MS010225 vs. ExPASy Swiss-Prot
Match: P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 879.4 bits (2271), Expect = 3.5e-254
Identity = 466/910 (51.21%), Postives = 613/910 (67.36%), Query Frame = 0

Query: 14  AAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCRREIKAGLSTSAKP 73
           A+ + +  ++RPS  S    AA        + + +   + R+ +VC           +  
Sbjct: 25  ASSSCIASLRRPSSSSSSVVAA--------ARRTRGQGSSRVVVVCASSSATASRGDSSS 84

Query: 74  TTSVQTKVVVKKITGLS-TSSQFLR---DVKDDVEKFLKLQFASNLLDPRTGSEKATIRV 133
             +    V VK +  +  T  + +    D++D + + L L+  S+ LD +TG EKAT+R 
Sbjct: 85  DMAAAAAVRVKAVATIKVTVGELINRSIDIRDLIGRSLSLELVSSELDAKTGKEKATVRS 144

Query: 134 EA-KLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTGL-ASGPST-- 193
            A  +  +D     YEA F+V S FG IGA+IV N   +EM++++++LT    +G ST  
Sbjct: 145 YAHNVDDDDHSVVTYEADFDVPSGFGPIGAIIVTNELRQEMFLEDINLTASDGAGNSTVL 204

Query: 194 -ITCNSWVQPPTVLPK---VQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKS 253
            I CNSWVQP +V  +    +R+FF +K+YLP QTPAGL+S R  +L   RG+GTGER++
Sbjct: 205 PIRCNSWVQPKSVGDEGTPSKRIFFANKTYLPGQTPAGLRSYRKNDLQQKRGDGTGEREA 264

Query: 254 TDNIYDYDVYNDLGDCTLE-ELRRPVLGGLER-PYPRRCRTGRPHCKKDPDTEEKPVGSM 313
            D +YDYDVYNDLG+     +L RPVLGG ++ PYPRRCRTGRP  KKDP +E +  G++
Sbjct: 265 DDRVYDYDVYNDLGNPDSNGDLARPVLGGNKQFPYPRRCRTGRPPSKKDPKSETRK-GNV 324

Query: 314 YVPRDQTFSNVKQAVF---TI-DLLRAAFPKLE-IFFDK---KATFSNFEEIDALFSRDG 373
           YVPRD+ FS  K+  F   T+  +L+AA P  + +  DK      F +F  ID LF  DG
Sbjct: 325 YVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSLLLDKLKWNLPFPSFFVIDKLF-EDG 384

Query: 374 FNLRNLESPTSLNDLLPWIFKLVSET-EEFFLHFQRPEPMDRDKFFWLSDEEFARETLAG 433
             L  ++    L  ++P + + + +T  E  L F+ P  + +DKF WL DEEFARETLAG
Sbjct: 385 VELPGVDKLNFLESVVPRLLEHLRDTPAEKILRFETPANIQKDKFAWLRDEEFARETLAG 444

Query: 434 LNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGS-MTVQEAIQKKKLFIVDYH 493
           +NPY+I+ V E+PL SKLDP +YGP ESA    +L++Q+   MTV+EAI +K+LF++D+H
Sbjct: 445 INPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFH 504

Query: 494 DMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQ 553
           D+ LPYV K+R+L  TT+YGSRT+FFL +D TL+ LAIELTRP    +PQW++V+ PS  
Sbjct: 505 DLFLPYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLLAIELTRPASPSQPQWRQVFTPSTD 564

Query: 554 ATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHF 613
           AT  W+WR AKAH   +DAG+H+L+THWLRTHC+VEPY IA NRQLS MHPIY+LL PHF
Sbjct: 565 ATMSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLRPHF 624

Query: 614 RYTMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLA 673
           RYTM INA ARS LI+A GIIE +FS +KYSMELSS AY   W+FD +ALPADL+ RG+A
Sbjct: 625 RYTMRINARARSALISAGGIIERSFSPQKYSMELSSVAYDKLWRFDTEALPADLVRRGMA 684

Query: 674 EEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTE 733
           EEDP A HGLKL I+DYPFANDGL++WDA+K WV  YV  +YPD   +  D ELQA+WTE
Sbjct: 685 EEDPTAEHGLKLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTE 744

Query: 734 IRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIA 793
           +RT GH DKK AP WP L++P+ L   +T I WVA+AHHAAVNFGQY + GYFPNRPSIA
Sbjct: 745 VRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIA 804

Query: 794 RTNMPTED-FNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTM 853
           RT MP E+  + A    FL+ P+  L + FPS  QAT VM VLDVLSSHS DEEY+G   
Sbjct: 805 RTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSSHSTDEEYLGGEQ 864

Query: 854 EPSWGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVT 899
              W  D  ++AA+D F+ ++KE+E +ID RN D  LKNR GAGI PY+L+KPFS  GVT
Sbjct: 865 TRPWNSDAAVQAAYDGFAARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDSGVT 924

BLAST of MS010225 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 1.4e-252
Identity = 449/840 (53.45%), Postives = 588/840 (70.00%), Query Frame = 0

Query: 80  KVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRTGSEKATIRVEA-KLTSEDL 139
           KV V+ +      S+ + +++D + + L L+  S+ L+ +TG +KAT+   A K+  +D 
Sbjct: 105 KVTVEGLLNSLRPSKAIDNIRDLIGRSLFLELVSSELEAKTGKKKATVHSYAHKVDDDDH 164

Query: 140 VEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLT-GLASGPST---ITCNSWVQP 199
               YEA F+V + FG IGAV+V N   +EM++++++LT G  +G ST   I CNSWVQP
Sbjct: 165 GVVTYEADFDVPTGFGPIGAVVVTNELGQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQP 224

Query: 200 PTVLPK---VQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKSTDNIYDYDVY 259
            + + +    +R+FF +K+YLP QTPAGL+S R  +L   RGNG G+R++ D +YDYDVY
Sbjct: 225 KSSIDEGTPGKRIFF-AKAYLPGQTPAGLRSYREEDLKQKRGNGAGQREADDRVYDYDVY 284

Query: 260 NDLGDCTLE-ELRRPVLGGLER-PYPRRCRTGRPHCKKDPDTEEKPVGSMYVPRDQTFSN 319
           NDLG+     +L RPVLGG ++ PYPRRCRTGRP  KKDP +E +  G++YVPRD+ FS 
Sbjct: 285 NDLGNPDSNGDLARPVLGGSKQFPYPRRCRTGRPPSKKDPKSETRK-GNVYVPRDEEFSE 344

Query: 320 VKQAVFTI----DLLRAAFPKLEIF----FDKKATFSNFEEIDALFSRDGFNLRNLESPT 379
           VK A F +     +L AA P  +            F +F  ID LF  DG  L  +E   
Sbjct: 345 VKNAQFLLKTLQSVLHAAVPAAQSALIDNLSLNLPFPSFFVIDKLF-EDGVELPGVEKLG 404

Query: 380 SLNDLLPWIFKLVSETE-EFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQSVT 439
            L+ ++P + +L+ ++  +  L F  P  + +DKF WL DEEFARETLAG+NPY+I+ V 
Sbjct: 405 FLHSIVPRLLELLRDSPGDKILLFDTPANVQKDKFAWLRDEEFARETLAGINPYAIELVR 464

Query: 440 EWPLMSKLDPEIYGPPESAFNTQMLDQQIGS-MTVQEAIQKKKLFIVDYHDMLLPYVQKV 499
           E+PL SKLDP +YGP ESA    +L++Q+   MTV+EAI +K+LF++D+HD+ LPYV K+
Sbjct: 465 EFPLKSKLDPAVYGPAESAITADLLEEQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKI 524

Query: 500 RALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFA 559
           R+LK TT+YGSRT+FFL +D TLR LAIELTRP    +PQW++V+ PS   T  W+WR A
Sbjct: 525 RSLKHTTMYGSRTIFFLTDDGTLRLLAIELTRPASPSQPQWRQVFTPSTDTTKSWLWRMA 584

Query: 560 KAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADA 619
           KAH   +DAG+H+L+THWLRTHC+VEPY IA NRQLS MHPIY+LLHPHFRYTM INA A
Sbjct: 585 KAHVRAHDAGHHELITHWLRTHCAVEPYIIAANRQLSEMHPIYQLLHPHFRYTMRINALA 644

Query: 620 RSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGL 679
           RS LI+A GIIE +FS +KYSMELSS AY   W+FD++ALPADL+ RG+AEEDP A HGL
Sbjct: 645 RSRLISAAGIIELSFSPQKYSMELSSVAYDKLWRFDMEALPADLVRRGMAEEDPTAEHGL 704

Query: 680 KLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKK 739
           +L I+DYPFANDGL++WDA+K WV  YV  +YPD   +  D ELQA+WTE+RT GH DKK
Sbjct: 705 RLAIEDYPFANDGLLIWDAIKTWVQAYVARFYPDADSVAGDEELQAFWTEVRTKGHGDKK 764

Query: 740 YAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTED-F 799
            AP WP L++P+ L   +T I WVA+AHHAAVNFGQY + GYFPNRPSIART MP E+  
Sbjct: 765 DAPWWPKLDSPESLAHTLTTIVWVAAAHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPV 824

Query: 800 NPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVI 859
           + A    FL+ P+  L + FPS  QAT VM VLDVLS+HS DEEY+G      W  D  +
Sbjct: 825 DGAAMERFLDNPDQALRECFPSQVQATVVMAVLDVLSTHSTDEEYLGGEQTRPWNSDAAV 884

Query: 860 KAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
           +AA+  F+ ++KE+E +ID RN D  LKNR GAGI PY+L+KPFS  GVTG G+P S SI
Sbjct: 885 QAAYAGFTARLKEIEGVIDGRNKDRKLKNRCGAGILPYQLMKPFSDAGVTGMGIPNSTSI 941

BLAST of MS010225 vs. ExPASy TrEMBL
Match: A0A6J1DLP5 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021206 PE=3 SV=1)

HSP 1 Score: 1839.7 bits (4764), Expect = 0.0e+00
Identity = 894/898 (99.55%), Postives = 896/898 (99.78%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR 60
           MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR
Sbjct: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSSNGRLSLVCR 60

Query: 61  REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT 120
           REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT
Sbjct: 61  REIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT 120

Query: 121 GSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTGLA 180
           GSEKATIRVEAKLTSEDLVEEIYEASF+VASDFGEIGAVIVENPNEKEMYIKEVDLTGLA
Sbjct: 121 GSEKATIRVEAKLTSEDLVEEIYEASFQVASDFGEIGAVIVENPNEKEMYIKEVDLTGLA 180

Query: 181 SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK 240
           SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK
Sbjct: 181 SGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERK 240

Query: 241 STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY 300
           STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY
Sbjct: 241 STDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPVGSMY 300

Query: 301 VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP 360
           VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP
Sbjct: 301 VPRDQTFSNVKQAVFTIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGFNLRNLESP 360

Query: 361 TSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQSVT 420
           TSLNDLLPW FKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQ VT
Sbjct: 361 TSLNDLLPWRFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQLVT 420

Query: 421 EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR 480
           EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR
Sbjct: 421 EWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVR 480

Query: 481 ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK 540
           ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK
Sbjct: 481 ALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAK 540

Query: 541 AHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR 600
           AHALVNDAGYHQL+THWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR
Sbjct: 541 AHALVNDAGYHQLITHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINADAR 600

Query: 601 SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK 660
           SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK
Sbjct: 601 SNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLK 660

Query: 661 LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY 720
           LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY
Sbjct: 661 LNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKY 720

Query: 721 APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP 780
           APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP
Sbjct: 721 APGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNP 780

Query: 781 ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA 840
           ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA
Sbjct: 781 ALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKA 840

Query: 841 AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
           AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI
Sbjct: 841 AFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 898

BLAST of MS010225 vs. ExPASy TrEMBL
Match: A0A6J1DHR2 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111021205 PE=3 SV=1)

HSP 1 Score: 1570.4 bits (4065), Expect = 0.0e+00
Identity = 771/906 (85.10%), Postives = 821/906 (90.62%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSS----NGRLS 60
           MLKGQGFG   RAAAPASLRH+QRP L SI+A AAF+P Y N   QKQKSS    NGRL 
Sbjct: 1   MLKGQGFGCSPRAAAPASLRHLQRPLLSSINAGAAFMPHYSNSRWQKQKSSKSSRNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LVC REIKA L+T+A+PTT+VQTKVVVKKITG  TSS  LRD K DVEKFLKL+FASN L
Sbjct: 61  LVC-REIKAALATAAEPTTTVQTKVVVKKITGEITSSTLLRDAK-DVEKFLKLEFASNQL 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           DPRTGSEK TIRVEAKL SEDLVEEIYEASFEV  DFGEIGAVIVENPNEKEMYIKEVDL
Sbjct: 121 DPRTGSEKPTIRVEAKLASEDLVEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
           TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT
Sbjct: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPV 300
           GERK+TD IYDYDVYNDLGDCT EEL+RPVLGG ERPYPRRCRTGRPHC +DPD+EE+  
Sbjct: 241 GERKATDRIYDYDVYNDLGDCTSEELKRPVLGGPERPYPRRCRTGRPHCTEDPDSEERTT 300

Query: 301 GSMYVPRDQTFSNVKQAVFTID----LLRAAFPKLEIFFDKKATFSNFEEIDALFSRDGF 360
           G++YVPRD+TFS +KQ  F +D    +LR   P+L++ FD+   F NF+ IDAL+  DGF
Sbjct: 301 GAIYVPRDETFSTIKQGAFNVDTILSVLRTILPRLQVHFDRDTGFKNFKSIDALYDVDGF 360

Query: 361 NLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLN 420
           NL  LES  SL DLLPWIFKLVSET EFFL FQ PEPMDRDKFFWLSDEEFAR+TLAGLN
Sbjct: 361 NLPPLESTDSLKDLLPWIFKLVSETGEFFLRFQSPEPMDRDKFFWLSDEEFARQTLAGLN 420

Query: 421 PYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDML 480
           P SIQ VTEWPL+SKLDPEIYGPPESAFNT+ML+QQIGSMTVQEAIQKKKL+I+DYHDML
Sbjct: 421 PCSIQLVTEWPLVSKLDPEIYGPPESAFNTEMLNQQIGSMTVQEAIQKKKLYILDYHDML 480

Query: 481 LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH 540
           LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH
Sbjct: 481 LPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQATH 540

Query: 541 LWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYT 600
           LW+WRFAKAH L +DAGYHQLV+HW+RTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRYT
Sbjct: 541 LWIWRFAKAHVLAHDAGYHQLVSHWVRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRYT 600

Query: 601 MEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEED 660
           +EINA ARSNLINA G IETTFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEED
Sbjct: 601 LEINAAARSNLINAGGTIETTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEED 660

Query: 661 PFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRT 720
           P ASHGL+LNIKDYPFANDGLILWDALK+WVTEYVNHYYPDPYLITSDTELQAWWTEIRT
Sbjct: 661 PTASHGLRLNIKDYPFANDGLILWDALKQWVTEYVNHYYPDPYLITSDTELQAWWTEIRT 720

Query: 721 VGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN 780
           VGHADKK A GWP LNTPQDLI+I++NIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN
Sbjct: 721 VGHADKKDAAGWPTLNTPQDLIEIVSNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTN 780

Query: 781 MPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSW 840
           MPTED NPALW+SFLEKPEDVLLDAFPS  QAT VM+VLDVLSSHSPDEEYIGK MEPSW
Sbjct: 781 MPTEDPNPALWKSFLEKPEDVLLDAFPSQYQATQVMMVLDVLSSHSPDEEYIGKNMEPSW 840

Query: 841 GDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGV 899
           GDDPVIKAAFD+F+ KMKELE+I+D RNAD +LKNRTGAG+TPYELLKPFSGPGVTGKGV
Sbjct: 841 GDDPVIKAAFDKFNAKMKELELIVDLRNADLDLKNRTGAGVTPYELLKPFSGPGVTGKGV 900

BLAST of MS010225 vs. ExPASy TrEMBL
Match: A0A6J1D3N9 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017305 PE=3 SV=1)

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 722/907 (79.60%), Postives = 789/907 (86.99%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSLCSIHAEAAFLPPYWNPSCQKQKSS----NGRLS 60
           MLKGQGFG  T AAAPASL H+QRP LCS +A AAFLP Y  PSCQKQ+SS    NGRL 
Sbjct: 1   MLKGQGFGCSTGAAAPASLSHLQRPLLCSTNAGAAFLPHYSKPSCQKQRSSKSSRNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LVC REIKA ++T+A+PTT+VQTKVVV+K+TG  TSS  +RDVK DVEKFLKL+FASNLL
Sbjct: 61  LVC-REIKAAIATAAEPTTTVQTKVVVQKVTGEITSSTIVRDVKPDVEKFLKLEFASNLL 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           D +TGSEKA I+VEA+L+SED  EEIYEASFEV  DFGEIGAVIVENPNEKEMYIKEVDL
Sbjct: 121 DLKTGSEKARIKVEARLSSEDPKEEIYEASFEVGPDFGEIGAVIVENPNEKEMYIKEVDL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
           TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLP  TPAGLKSMRA ELVNLRGNGT
Sbjct: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPLLTPAGLKSMRANELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKPV 300
           GER   D IYDYDVYNDLG C+  +L+RPVLGG E PYPRRCRTGRPHC+  P+ EE+  
Sbjct: 241 GERVPYDRIYDYDVYNDLGSCS-GDLKRPVLGGAEHPYPRRCRTGRPHCENSPELEERRK 300

Query: 301 G-SMYVPRDQTFSNVKQAVF----TIDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDG 360
             S+Y+PRD+ FSN+KQ  F     +  +    P L + FD KA FSNF++IDAL++ DG
Sbjct: 301 NESIYIPRDEAFSNIKQDAFMKDSVLSFIHTIVPNLWVHFDDKAGFSNFKDIDALYAVDG 360

Query: 361 FNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGL 420
           F L  LES TSL D LPWIFKL S T +  L F  PEPMDRDKFFWLSDE+FAR+TLAGL
Sbjct: 361 FKLPTLESSTSLKDDLPWIFKLDS-TGKISLRFDPPEPMDRDKFFWLSDEQFARQTLAGL 420

Query: 421 NPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDM 480
           NP SIQ VTEWPL+SKLDPEIYGPPESAFN +ML+Q+IGS+TVQEAIQKKKL+I+DYHDM
Sbjct: 421 NPCSIQLVTEWPLVSKLDPEIYGPPESAFNIEMLNQKIGSITVQEAIQKKKLYILDYHDM 480

Query: 481 LLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQAT 540
           LLPYVQKVRA++GTTLYGSRTLFFLNEDETL+PLAIELTRPPMDDKPQWKRVYGPSEQAT
Sbjct: 481 LLPYVQKVRAVEGTTLYGSRTLFFLNEDETLKPLAIELTRPPMDDKPQWKRVYGPSEQAT 540

Query: 541 HLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRY 600
           HLW+WRFAKAH L +DAGYHQLV+HWLRTHC+VEPYCIATNRQLSAMHPIYRLLHPHFRY
Sbjct: 541 HLWIWRFAKAHVLAHDAGYHQLVSHWLRTHCAVEPYCIATNRQLSAMHPIYRLLHPHFRY 600

Query: 601 TMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEE 660
           T+EINA AR+NLI+A G IE TFS +KYSMELSSTAY LQWQFDLQALPADLIHRGLAEE
Sbjct: 601 TLEINASARTNLISAGGTIEFTFSPQKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEE 660

Query: 661 DPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIR 720
           DP ASHGLKLNIKDYPFANDGLILWDALKEWVTEYVN+YY D  ++TSD ELQ WWTEIR
Sbjct: 661 DPTASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNYYYHDSSMVTSDAELQEWWTEIR 720

Query: 721 TVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIART 780
           TVGHADKK A GWP LNTPQDLIDI+TNIAWVAS HHAAVNFGQYAYAGYFPNRPSI +T
Sbjct: 721 TVGHADKKDAAGWPTLNTPQDLIDIVTNIAWVASGHHAAVNFGQYAYAGYFPNRPSITKT 780

Query: 781 NMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPS 840
           NMPTED NPALW+ FLEKPE  LLDAFPS  QAT VM+VL++LSSHSPDEEY+GK MEPS
Sbjct: 781 NMPTEDPNPALWKCFLEKPEHFLLDAFPSQYQATKVMMVLNILSSHSPDEEYVGKNMEPS 840

Query: 841 WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKG 899
           WGDDPVIKAAFDRFS KMK LEVIID RNAD +LKNRTGAG++PYELLKPFS  GVTGKG
Sbjct: 841 WGDDPVIKAAFDRFSAKMKNLEVIIDRRNADFSLKNRTGAGVSPYELLKPFSESGVTGKG 900

BLAST of MS010225 vs. ExPASy TrEMBL
Match: A0A6J1JXK9 (Lipoxygenase OS=Cucurbita maxima OX=3661 GN=LOC111490700 PE=3 SV=1)

HSP 1 Score: 1392.5 bits (3603), Expect = 0.0e+00
Identity = 689/907 (75.96%), Postives = 777/907 (85.67%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSL-CSIHAEAAFLPPYWNPSCQKQKS---SNGRLS 60
           MLK QG  GC+   APA+L H+QRP L   I+A  +F  P+WN    KQK+   SNGRL 
Sbjct: 1   MLKSQGV-GCSTRVAPATLCHLQRPLLGRGINAGVSF--PFWNNPGNKQKAKSISNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LV  REIKA L+T A+PTT+V TKV+VKKITG  TSS  L + K  ++K LKL+FAS L+
Sbjct: 61  LV-SREIKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESK-ALDKLLKLEFASILV 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           DPRTG EK  I V+AKL SED++EEIYEAS EV +DFG+IGAVIVEN NEKEM++KEV+L
Sbjct: 121 DPRTGLEKPPISVQAKLISEDVLEEIYEASLEVPADFGDIGAVIVENHNEKEMFVKEVEL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
            GLASGP  +TCNSWVQP T++P   RVFFT KSYLP QTPAGL+SMR  ELVNLRGNGT
Sbjct: 181 NGLASGPLIVTCNSWVQPKTLVPTQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKP 300
           GER+S D +YDYDVYNDLGD    E+L+RPVLGG ERPYPRRCRTGRP  K DP++E++ 
Sbjct: 241 GERRSYDRVYDYDVYNDLGDPDKSEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRA 300

Query: 301 VGSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDG 360
            GS+YVPRD+TFS++KQ VF     + +LRA  P+L++ FD+   F NF+ +DALF  DG
Sbjct: 301 TGSIYVPRDETFSDLKQGVFNASTLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDG 360

Query: 361 FNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGL 420
           FNL  LES TSL DLLPWIFK +SET +F   FQ PEPMDRDKFFWL DEEFAR+TLAGL
Sbjct: 361 FNLPPLESTTSLKDLLPWIFKFLSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGL 420

Query: 421 NPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDM 480
           NP SIQ V EWPLMS+L+PEIYGPPESAFNTQM+D++IGSMTVQEAIQ+KKLFI+DY+DM
Sbjct: 421 NPCSIQLVKEWPLMSQLNPEIYGPPESAFNTQMIDEEIGSMTVQEAIQEKKLFILDYNDM 480

Query: 481 LLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQAT 540
           LLPYV++VRALKGTTLYGSRTLFFLNED TLRPLAIELTRPPMD KPQWK VYGPSE AT
Sbjct: 481 LLPYVRRVRALKGTTLYGSRTLFFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENAT 540

Query: 541 HLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRY 600
           +LW+WRFAK+H L +DAGYHQLV+HWLRTHC+VEPY IATNRQLSAMHPI+RLL+PHFRY
Sbjct: 541 NLWLWRFAKSHVLAHDAGYHQLVSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRY 600

Query: 601 TMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEE 660
           TMEINA AR+NLINA G IE+TFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEE
Sbjct: 601 TMEINAAARTNLINAGGTIESTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEE 660

Query: 661 DPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIR 720
           DP ASHGLKL+IKDYPFANDGLILWDALK+WVTEYVNHYYPDP L+ SDTELQAWWTEIR
Sbjct: 661 DPTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNHYYPDPSLVASDTELQAWWTEIR 720

Query: 721 TVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIART 780
           TVGHADK+  P WP LNTPQDLIDI+TNIAWVASAHHAAVNFGQYAY GYFP+RPSIART
Sbjct: 721 TVGHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYGGYFPSRPSIART 780

Query: 781 NMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPS 840
           NMPTED NPALW+SFLEKPE+VLL+AFPS  QAT VMLVLDVLSSHSPDEEY+GK MEPS
Sbjct: 781 NMPTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPS 840

Query: 841 WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKG 899
           WGD+P IKAAFDRF+ +MKELE+IID+RNAD NLKNRTGAG+TPYELLKPFSGPGVTGKG
Sbjct: 841 WGDNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKG 900

BLAST of MS010225 vs. ExPASy TrEMBL
Match: A0A6J1H2B7 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459461 PE=3 SV=1)

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 689/907 (75.96%), Postives = 778/907 (85.78%), Query Frame = 0

Query: 1   MLKGQGFGGCTRAAAPASLRHMQRPSL-CSIHAEAAFLPPYWNPSCQKQKS---SNGRLS 60
           MLK QG  GC+   APA+L H+QRPSL   I+A  +F  P+WN    KQK+   SNGRL 
Sbjct: 1   MLKSQGV-GCSTRVAPATLCHLQRPSLGRGINAGVSF--PFWNNPGNKQKAKSISNGRLR 60

Query: 61  LVCRREIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLL 120
           LV  REIKA L+T A+PTT+V TKV+VKKITG  TSS  L + K  ++K LKL+FAS L+
Sbjct: 61  LV-SREIKAALTTVAEPTTTVLTKVIVKKITGEITSSTLLMESK-ALDKLLKLEFASILV 120

Query: 121 DPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDL 180
           DP+TG EK  I V+AKL SED++EEIYEAS EV +DFG+IGAVIVEN NEKE ++KEV+L
Sbjct: 121 DPKTGLEKPPISVQAKLISEDVLEEIYEASLEVPADFGDIGAVIVENHNEKEKFVKEVEL 180

Query: 181 TGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGT 240
            GLASGP  +TCNSWVQP T++P   RVFFT KSYLP QTPAGL+SMR  ELVNLRGNGT
Sbjct: 181 NGLASGPLIVTCNSWVQPKTLVPTQNRVFFTDKSYLPSQTPAGLRSMRETELVNLRGNGT 240

Query: 241 GERKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTEEKP 300
           GER+S D IYDYDVYNDLGD    E+L+RPVLGG ERPYPRRCRTGRP  K DP++E++ 
Sbjct: 241 GERQSYDRIYDYDVYNDLGDPDKSEDLKRPVLGGTERPYPRRCRTGRPPTKTDPNSEQRA 300

Query: 301 VGSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKLEIFFDKKATFSNFEEIDALFSRDG 360
            GS+YVPRD+TFS++KQ VF     + +LRA  P+L++ FD+   F NF+ +DALF  DG
Sbjct: 301 TGSIYVPRDETFSDLKQGVFNASTLLSVLRAIIPRLQVHFDQNTGFPNFKAVDALFDVDG 360

Query: 361 FNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARETLAGL 420
           FNL  LES TSL DLLPWIFK +SET +F   FQ PEPMDRDKFFWL DEEFAR+TLAGL
Sbjct: 361 FNLPPLESTTSLKDLLPWIFKFLSETGQFLFRFQSPEPMDRDKFFWLRDEEFARQTLAGL 420

Query: 421 NPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDM 480
           NP SIQ V EWPLMS+L+PEIYGPPESAFNTQM+D++IGSMTVQEAIQ+KKLFI+DY+DM
Sbjct: 421 NPCSIQLVKEWPLMSQLNPEIYGPPESAFNTQMIDREIGSMTVQEAIQQKKLFILDYNDM 480

Query: 481 LLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPSEQAT 540
           LLPYV++VRALKGTTLYGSRTLFFLNED TLRPLAIELTRPPMD KPQWK VYGPSE AT
Sbjct: 481 LLPYVRRVRALKGTTLYGSRTLFFLNEDGTLRPLAIELTRPPMDGKPQWKGVYGPSENAT 540

Query: 541 HLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRY 600
           +LW+WRFAK+H L +DAGYHQLV+HWLRTHC+VEPY IATNRQLSAMHPI+RLL+PHFRY
Sbjct: 541 NLWLWRFAKSHVLAHDAGYHQLVSHWLRTHCAVEPYVIATNRQLSAMHPIHRLLNPHFRY 600

Query: 601 TMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEE 660
           TMEINA AR+NLINA G IE+TFS  KYSMELSSTAY LQWQFDLQALPADLIHRGLAEE
Sbjct: 601 TMEINAAARTNLINAGGTIESTFSPLKYSMELSSTAYDLQWQFDLQALPADLIHRGLAEE 660

Query: 661 DPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIR 720
           DP ASHGLKL+IKDYPFANDGLILWDALK+WVTEYVNHYYPDP L+ SDTELQ+WWTEIR
Sbjct: 661 DPTASHGLKLHIKDYPFANDGLILWDALKQWVTEYVNHYYPDPSLVASDTELQSWWTEIR 720

Query: 721 TVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIART 780
           TVGHADK+  P WP LNTPQDLIDI+TNIAWVASAHHAAVNFGQYAYAGYFP+RPSIART
Sbjct: 721 TVGHADKQDEPWWPLLNTPQDLIDIVTNIAWVASAHHAAVNFGQYAYAGYFPSRPSIART 780

Query: 781 NMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPS 840
           NMPTED NPALW+SFLEKPE+VLL+AFPS  QAT VMLVLDVLSSHSPDEEY+GK MEPS
Sbjct: 781 NMPTEDTNPALWKSFLEKPEEVLLNAFPSQYQATQVMLVLDVLSSHSPDEEYLGKNMEPS 840

Query: 841 WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKG 899
           WGD+P IKAAFDRF+ +MKELE+IID+RNAD NLKNRTGAG+TPYELLKPFSGPGVTGKG
Sbjct: 841 WGDNPAIKAAFDRFNKRMKELELIIDHRNADFNLKNRTGAGVTPYELLKPFSGPGVTGKG 900

BLAST of MS010225 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 891.7 bits (2303), Expect = 4.8e-259
Identity = 453/873 (51.89%), Postives = 580/873 (66.44%), Query Frame = 0

Query: 42  NPSCQKQKSSNGRLSLVCRREIKAGLSTSAKPTTSVQTKVVVKKITG-LSTSSQFLRDVK 101
           NP    ++++  R +L  R ++ A  +   +   +V+  +   K+ G ++   +FL  + 
Sbjct: 31  NPISAGRRNNLPRPNLRRRCKVTASRANIEQEGNTVKEPIQNIKVKGYITAQEEFLEGIT 90

Query: 102 -----DDVEKFLKLQFASNLLDPRTGSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGE 161
                DD+           L+  +T  ++ T+   A+    +  +E YE  FE+  DFG 
Sbjct: 91  WSRGLDDIADIRGRSLLVELISAKT-DQRITVEDYAQRVWAEAPDEKYECEFEMPEDFGP 150

Query: 162 IGAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQ 221
           +GA+ ++N   +++++K V+L  L  G  T TC SWV P +V P  +R+FF+ KSYLP Q
Sbjct: 151 VGAIKIQNQYHRQLFLKGVELK-LPGGSITFTCESWVAPKSVDP-TKRIFFSDKSYLPSQ 210

Query: 222 TPAGLKSMRAAELVNLRGNG---TGERKSTDNIYDYDVYNDLGDCTLE-ELRRPVLGGLE 281
           TP  LK  R  EL  L+G      GE    + IYDYDVYND+GD   + EL RPV+GGL 
Sbjct: 211 TPEPLKKYRKEELETLQGKNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLT 270

Query: 282 RPYPRRCRTGRPHCKKDPDTEEKPVGSMYVPRDQTFSNVKQAVFT----IDLLRAAFPKL 341
            PYPRRC+TGR  C+ DP +E++  G  YVPRD+ FS  K   FT    +  L + FP++
Sbjct: 271 HPYPRRCKTGRKPCETDPSSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQI 330

Query: 342 E-IFFDKKATFSNFEEIDALFSRDGFNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQR 401
           E +    +  F +F+ I  LF  +G     L     L  LLP I K + E ++  L F  
Sbjct: 331 ESVLLSPQEPFPHFKAIQNLF-EEGI---QLPKDAGLLPLLPRIIKALGEAQDDILQFDA 390

Query: 402 PEPMDRDKFFWLSDEEFARETLAGLNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLD 461
           P  ++RD+F WL D+EFAR+TLAGLNPYSIQ V EWPL+SKLDP +YG P S    ++++
Sbjct: 391 PVLINRDRFSWLRDDEFARQTLAGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVE 450

Query: 462 QQI-GSMTVQEAIQKKKLFIVDYHDMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPL 521
           +++ G+MTV EA++ K+LF++DYHD+LLPYV KVR L  TTLY SRTLFFL++D TLRP+
Sbjct: 451 REVKGNMTVDEALKNKRLFVLDYHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPV 510

Query: 522 AIELTRPPMDDKPQWKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVE 581
           AIELT PP  +KPQWK+V+ P   AT  W+W  AK HA+ +DAGYHQL++HWLRTH   E
Sbjct: 511 AIELTCPPNINKPQWKQVFTPGYDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTE 570

Query: 582 PYCIATNRQLSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTFSTKKYSMELSS 641
           PY IA NRQLSAMHPIYRLLHPHFRYTMEINA AR +L+N  GIIET F   KY++ELSS
Sbjct: 571 PYIIAANRQLSAMHPIYRLLHPHFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSS 630

Query: 642 TAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTE 701
             Y   W+FD + LPADLI RGLAEED  A HG++L I DYPFANDGLILWDA+KEWVT+
Sbjct: 631 AVYGKLWRFDQEGLPADLIKRGLAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTD 690

Query: 702 YVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVAS 761
           YV HYYPD  LITSD ELQ WW+E+R +GH DKK  P WP L T  DLI ++T IAWV S
Sbjct: 691 YVKHYYPDEELITSDEELQGWWSEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTS 750

Query: 762 AHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQAT 821
            HHAAVNFGQY Y GYFPNRP+  R  MPTED      + F E PE VLL  +PS  QAT
Sbjct: 751 GHHAAVNFGQYGYGGYFPNRPTTTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQAT 810

Query: 822 TVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNL 881
            VM+ LD+LS+HSPDEEYIG+  E SW ++PVI AAF+RF  K++ LE +ID RN +  L
Sbjct: 811 LVMVTLDLLSTHSPDEEYIGEQQEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITL 870

Query: 882 KNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
           KNR GAG+  YELLKP S  GVTG GVP S+SI
Sbjct: 871 KNRAGAGVVKYELLKPTSEHGVTGMGVPYSISI 896

BLAST of MS010225 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 733.4 bits (1892), Expect = 2.2e-211
Identity = 385/814 (47.30%), Postives = 521/814 (64.00%), Query Frame = 0

Query: 101 DDVEKFLKLQFASNLLDPRTG----SEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEI 160
           D + + + L+  S  +DP+T     S+ A ++  +K ++       Y A F V S FG  
Sbjct: 119 DKIGRNVVLELMSTQVDPKTNEPKKSKAAVLKDWSKKSNSKAERVHYTAEFTVDSAFGSP 178

Query: 161 GAVIVENPNEKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQT 220
           GA+ V N ++KE +++ + + G A GP    CNSWVQ     P  +R+ FT++ YLP +T
Sbjct: 179 GAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPS-KRILFTNQPYLPSET 238

Query: 221 PAGLKSMRAAELVNLRGNGTGERKSTDNIYDYDVYNDLGDCTL-EELRRPVLGGLERPYP 280
           P+GL+++R  EL NLRGNG GERK +D IYDYDVYND+G+  +  EL RP LGG E PYP
Sbjct: 239 PSGLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISRELARPTLGGREFPYP 298

Query: 281 RRCRTGRPHCKKDPDTE---EKPVGSMYVPRDQTFSNVKQAVFTIDLLRAA----FPKLE 340
           RRCRTGR     D  +E   EKP+  MYVPRD+ F   KQ  F    L+A      P L+
Sbjct: 299 RRCRTGRSSTDTDMMSERRVEKPL-PMYVPRDEQFEESKQNTFAACRLKAVLHNLIPSLK 358

Query: 341 IFFDKKATFSNFEEIDALFSRDGFNLR-NLESPTSLNDLLPWIFKLVSETEEFFLHFQRP 400
                   F+NF EID+L+ ++G  L+   +        LP I   + ++ E  L +  P
Sbjct: 359 ASI-LAEDFANFGEIDSLY-KEGLLLKLGFQDDMFKKFPLPKIVTTLQKSSEGLLRYDTP 418

Query: 401 EPMDRDKFFWLSDEEFARETLAGLNPYSIQSVTEWPLMSKLDPEIYGPP-ESAFNTQMLD 460
           + + +DK+ WL D+EFAR+ +AG+NP +I+ VT +P +S LDPEIYGP   SA     + 
Sbjct: 419 KIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLDPEIYGPGLHSALTEDHII 478

Query: 461 QQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLA 520
            Q+  +TVQ+A++  +LF+VDYHD+ LP++ ++ AL G   Y +RT+ FL    TL+P+A
Sbjct: 479 GQLDGLTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKAYATRTILFLTRLGTLKPIA 538

Query: 521 IELTRPPMDDKPQ-WKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVE 580
           IEL+ P      Q  KRV  P   AT  W+W+ AKAH   NDAG HQLV HWLRTH  +E
Sbjct: 539 IELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSNDAGVHQLVNHWLRTHACLE 598

Query: 581 PYCIATNRQLSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTFSTKKYSMELSS 640
           P+ +A +RQLSAMHPI++LL PH RYT+EINA AR  LI+ADG+IE+ F+  +Y +E+SS
Sbjct: 599 PFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISADGVIESCFTAGQYGLEISS 658

Query: 641 TAYHLQWQFDLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTE 700
            AY  +W+FD++ LPADLI RG+A  DP   HGLKL ++DYP+ANDGL+LW A++ WV  
Sbjct: 659 AAYKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDYPYANDGLLLWSAIQTWVRT 718

Query: 701 YVNHYYPDPYLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVAS 760
           YV  YY +  LI +DTELQAW++E   VGHAD + A  WP L+T +DL+ +IT I W+AS
Sbjct: 719 YVERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPKLSTVEDLVSVITTIIWLAS 778

Query: 761 AHHAAVNFGQYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQAT 820
           A HAA+NFGQY Y GY PNRP + R  +P E  +   + SF+E P+     + PS  Q T
Sbjct: 779 AQHAALNFGQYPYGGYVPNRPPLMRRLIPDE--SDPEFTSFIEDPQKYFFSSMPSLLQTT 838

Query: 821 TVMLVLDVLSSHSPDEEYIGKTMEPS-WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCN 880
             M V+D LS+HSPDEEYIG+  +PS W  D  I  AF  FS ++  +E  ID RN D +
Sbjct: 839 KFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFSAEIGRIEKEIDKRNRDPS 898

Query: 881 LKNRTGAGITPYELLKPFSGPGVTGKGVPCSVSI 899
            +NR GAG+ PYEL+ P S PGVT +GVP SVSI
Sbjct: 899 RRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

BLAST of MS010225 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 714.5 bits (1843), Expect = 1.0e-205
Identity = 385/852 (45.19%), Postives = 529/852 (62.09%), Query Frame = 0

Query: 62  EIKAGLSTSAKPTTSVQTKVVVKKITGLSTSSQFLRDVKDDVEKFLKLQFASNLLDPRT- 121
           E KA +    +   +V+ K    K     T  + L    D + + + L+  S  LDP+T 
Sbjct: 77  EEKAAVKFKVRAVVTVRNK---NKEDLKETLVKHLDAFADKIGRNIVLELISTQLDPKTK 136

Query: 122 ---GSEKATIRVEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLT 181
               S  A ++  +K +        Y A F V + FG  GA+ V N ++KE +++ + + 
Sbjct: 137 LPKKSNAAVLKDWSKKSKTKAERVHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIE 196

Query: 182 GLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTG 241
           G A GP    CNSWVQ     P  +R+FFT++ YLP +TP+GL+ +R  EL NLRG+G+G
Sbjct: 197 GFALGPVHFPCNSWVQSQKDHPD-KRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSG 256

Query: 242 ERKSTDNIYDYDVYNDLGD-CTLEELRRPVLGGLERPYPRRCRTGRPHCKKDPDTE---E 301
            RK +D IYD+DVYNDLG+     EL RP LGG E PYPRRCRTGR     D D E   E
Sbjct: 257 VRKLSDRIYDFDVYNDLGNPDKSSELSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVE 316

Query: 302 KPVGSMYVPRDQTFSNVKQAVFTIDLLRAA----FPKLEIFFDKKATFSNFEEIDALFSR 361
           KP+  MYVPRD+ F   KQ  F    L+A      P L+     +  F++F EID L+ +
Sbjct: 317 KPL-PMYVPRDEQFEESKQDTFAAGRLKAVLHHLIPSLKASIVAE-DFADFGEIDRLY-K 376

Query: 362 DGFNLR-NLESPTSLNDLLP-WIFKLVSETEEFFLHFQRPEPMDRDKFFWLSDEEFARET 421
           +G  L+   +        LP  +   + E+ +  L +  P+ + +DK  WL D+EFAR+ 
Sbjct: 377 EGLLLKLGFQDDIFKKFPLPKVVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQA 436

Query: 422 LAGLNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVD 481
           +AG+NP +I+ V  +P +S LDP+IYGP  SA     +   +   +VQ+A+++ +L+++D
Sbjct: 437 IAGINPVNIERVKTFPPVSNLDPKIYGPQHSALTDDHIIGHLDGFSVQQALEENRLYMLD 496

Query: 482 YHDMLLPYVQKVRALKGTTLYGSRTLFFLNEDETLRPLAIELTRPPMDDKPQWKRVYGPS 541
           YHD+ LP++ ++ AL G   Y +RT+FFL    TL+P+AIEL+ PP   K + KRV  P 
Sbjct: 497 YHDIFLPFLDRINALDGRKAYATRTIFFLTRLGTLKPVAIELSLPPHGPKHRSKRVLTPP 556

Query: 542 EQATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHP 601
             AT  W+W+ AKAH   NDAG HQLV HWLRTH  +EP+ +A +RQLSAMHPI++LL P
Sbjct: 557 VDATSNWMWQLAKAHVSSNDAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDP 616

Query: 602 HFRYTMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRG 661
           H RYT+EINA AR +LI+ADG+IE  F+   Y ME+S+ AY   W+FD++ LPADLI RG
Sbjct: 617 HMRYTLEINALARQSLISADGVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRG 676

Query: 662 LAEEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWW 721
           +A  D    HGLKL I+DYP+ANDGL+LW A++ WV  YV  YYP+P LI +D+ELQ+W+
Sbjct: 677 MAIPDATQPHGLKLLIEDYPYANDGLLLWSAIQTWVRTYVERYYPNPNLIKTDSELQSWY 736

Query: 722 TEIRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPS 781
           +E   VGHAD + A  WP L+T  DL+ I+T + W+ASA HAA+NFGQY Y GY PNRP 
Sbjct: 737 SESINVGHADLRDADWWPELSTVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPP 796

Query: 782 IARTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKT 841
           + R  +P E  +   + SF+  PE     + PS  Q +  M V+D LS+HSPDEEYIG+ 
Sbjct: 797 LMRRLIPDE--SDPEYASFISHPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGER 856

Query: 842 MEPS-WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPG 899
            +PS W  D  I  AF  F+ ++  +E  I+ RNAD + +NR GAG+ PYELL P S PG
Sbjct: 857 QQPSIWTGDAEIVEAFYGFAAEIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPG 916

BLAST of MS010225 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 696.4 bits (1796), Expect = 2.9e-200
Identity = 362/806 (44.91%), Postives = 494/806 (61.29%), Query Frame = 0

Query: 109 LQFASNLLDPRTGSEKATIR---VEAKLTSEDLVEEIYEASFEVASDFGEIGAVIVENPN 168
           +Q  S  +DP TG  + ++    +      +D    ++ A F V  +FG+ GA++V N  
Sbjct: 117 IQLVSEEIDPETGKGRKSLESPVMGLPKAVKDPRYLVFTADFTVPINFGKPGAILVTNLL 176

Query: 169 EKEMYIKEVDLTGLASGPSTITCNSWVQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRA 228
             E+ + E+ +   ++       N+W+      P+  R+ F S+  LP +TP G+K +R 
Sbjct: 177 STEICLSEIIIED-STDTILFPANTWIHSKNDNPQA-RIIFRSQPCLPSETPDGIKELRE 236

Query: 229 AELVNLRGNGTGERKSTDNIYDYDVYNDLGDCTLEELRRPVLGGLERPYPRRCRTGRPHC 288
            +LV++RG+G GERK  + IYDYDVYNDLGD    E  RPVLG  E PYPRRCRTGRP  
Sbjct: 237 KDLVSVRGDGKGERKPHERIYDYDVYNDLGDPRKTERVRPVLGVPETPYPRRCRTGRPLV 296

Query: 289 KKDPDTEE--KPVGSMYVPRDQTFSNVKQAVFTIDLLRAAFPKL-----EIFFDKKATFS 348
            KDP  E   K     YVPRD+ F  +K+  F     +A F  L         +    F+
Sbjct: 297 SKDPPCESRGKEKEEFYVPRDEVFEEIKRDTFRAGRFKALFHNLVPSIAAALSNLDIPFT 356

Query: 349 NFEEIDALFSRDGFNLRNLESPTSLNDLLPWIFKLVSETEEFFLHFQRPEPMDRDKFFWL 408
            F +ID L+  +          T L   +      +    E  L +  P  +  D+F WL
Sbjct: 357 CFSDIDNLYKSNIVLGHTEPKDTGLGGFIGGFMNGILNVTETLLKYDTPAVIKWDRFAWL 416

Query: 409 SDEEFARETLAGLNPYSIQSVTEWPLMSKLDPEIYGPPESAFNTQMLDQQIG--SMTVQE 468
            D EF R+ LAG+NP +I+ + E P+ S LDP +YGP ES    +++ +++     T+++
Sbjct: 417 RDNEFGRQALAGVNPVNIELLKELPIRSNLDPALYGPQESVLTEEIIAREVEHYGTTIEK 476

Query: 469 AIQKKKLFIVDYHDMLLPYVQKVRALK--GTTLYGSRTLFFLNEDETLRPLAIELTRPPM 528
           A+++K+LF+VDYHD+LLP+V+K+ ++K      Y SRT+FF +++  LRPLAIEL+ PP 
Sbjct: 477 ALEEKRLFLVDYHDILLPFVEKINSIKEDPRKTYASRTIFFYSKNGALRPLAIELSLPPT 536

Query: 529 DDKPQWKRVYGPSEQATHLWVWRFAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQ 588
            +    K VY     AT  W+W+ AKAH   NDAG HQLV HWLRTH S+EPY IATNRQ
Sbjct: 537 AESEN-KFVYTHGHDATTHWIWKLAKAHVCSNDAGVHQLVNHWLRTHASMEPYIIATNRQ 596

Query: 589 LSAMHPIYRLLHPHFRYTMEINADARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQF 648
           LS MHP+Y+LLHPH RYT+EINA AR +LIN  GIIE+ F+  KY+MELSS AY   W+F
Sbjct: 597 LSTMHPVYKLLHPHMRYTLEINARARKSLINGGGIIESCFTPGKYAMELSSAAYKSMWRF 656

Query: 649 DLQALPADLIHRGLAEEDPFASHGLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDP 708
           D++ LPADL+ RG+AEED  A  G++L I DYP+A DGL++W A+K+ V  YV H+Y D 
Sbjct: 657 DMEGLPADLVRRGMAEEDSSAECGVRLVIDDYPYAADGLLIWKAIKDLVESYVKHFYSDS 716

Query: 709 YLITSDTELQAWWTEIRTVGHADKKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFG 768
             ITSD ELQAWW EI+  GH DKK  P WP LNT QDL  I+TN+ W+AS  HAA+NFG
Sbjct: 717 KSITSDLELQAWWDEIKNKGHYDKKDEPWWPKLNTTQDLSQILTNMIWIASGQHAAINFG 776

Query: 769 QYAYAGYFPNRPSIARTNMPTEDFNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVL 828
           QY + GY PNRP++ R  +P E      +  F+  P+   L + P+  QAT VM V + L
Sbjct: 777 QYPFGGYVPNRPTLLRKLIPQE--TDPDYEMFMRNPQYSFLGSLPTQLQATKVMAVQETL 836

Query: 829 SSHSPDEEYIGKTMEPS--WGDDPVIKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAG 888
           S+HSPDEEY+ +  E    W  D  +   F++FS ++ ++E  I+ RN D  LKNRTGAG
Sbjct: 837 STHSPDEEYLIELREVQRHWFQDEQVVKYFNKFSEELVKIEKTINERNKDKKLKNRTGAG 896

Query: 889 ITPYELLKPFSGPGVTGKGVPCSVSI 899
           + PYELL P S  GVTG+G+P S+SI
Sbjct: 897 MPPYELLLPTSPHGVTGRGIPNSISI 917

BLAST of MS010225 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 631.7 bits (1628), Expect = 8.9e-181
Identity = 356/781 (45.58%), Postives = 475/781 (60.82%), Query Frame = 0

Query: 133 LTSEDLVEEIYEASFEVASDFGEIGAVIVENPNEKEMYIKEVDLTGL-ASGPSTITCNSW 192
           +TS    E  ++ +F+  +DFG  GA ++ N +  E  +K + L  +   G     CNSW
Sbjct: 87  ITSLTAGESAFKVTFDYETDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGRVHYICNSW 146

Query: 193 VQPPTVLPKVQRVFFTSKSYLPFQTPAGLKSMRAAELVNLRGNGTGERKSTDNIYDYDVY 252
           + P        RVFF++K+YLP +TPA L   R  ELV+LRG G GE K  D +YDY  Y
Sbjct: 147 IYPAKHY-TTDRVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDRVYDYAYY 206

Query: 253 NDLGDCTLEELRRPVLGGL-ERPYPRRCRTGRPHCKKDPDTEEK-PVGS---MYVPRDQT 312
           NDLG     +  RPVLGG  E PYPRR RTGR   K+DP TE + P+ S   +YVPRD+ 
Sbjct: 207 NDLG--VPPKNPRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDER 266

Query: 313 FSNVKQAVFTIDLLRAAF----PKLE-IFFDKKATFSNFEEIDALFSRDGFNLRNLESPT 372
           F ++K + F    L+A      P LE +F D    F +FE++  ++  +G +L N     
Sbjct: 267 FGHLKMSDFLAYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIY-EEGIDLPNQALID 326

Query: 373 SLNDLLPW-IFKLVSETE-EFFLHFQRPEPMDRDKFFWLSDEEFARETLAGLNPYSIQSV 432
           S+   +P  + K +  T+ + FL F  P+ +  DK  W +DEEFARE LAGLNP  IQ +
Sbjct: 327 SIVKNIPLEMLKEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLL 386

Query: 433 TEWPLMSKLDPEIYGPPESAFNTQMLDQQIGSMTVQEAIQKKKLFIVDYHDMLLPYVQKV 492
            E+P  SKLD E YG   S      ++  +  +TV+EA++K++LFI+D+HD L+PY+ +V
Sbjct: 387 KEFPPKSKLDSESYGNQNSTITKSHIEHNLDGLTVEEALEKERLFILDHHDTLMPYLGRV 446

Query: 493 RALKGTTLYGSRTLFFLNEDETLRPLAIELTRP-PMDDK-PQWKRVYGPSEQATHLWVWR 552
                T  Y SRTL FL +D TL+PL IEL+ P P  DK      VY P E   +  +W+
Sbjct: 447 NTTT-TKTYASRTLLFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTPGE-GVYDSLWQ 506

Query: 553 FAKAHALVNDAGYHQLVTHWLRTHCSVEPYCIATNRQLSAMHPIYRLLHPHFRYTMEINA 612
            AKA   VND+G HQL++HW++TH S+EP+ IATNRQLS +HP+++LL PHFR TM INA
Sbjct: 507 LAKAFVGVNDSGNHQLISHWMQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINA 566

Query: 613 DARSNLINADGIIETTFSTKKYSMELSSTAYHLQWQFDLQALPADLIHRGLAEEDPFASH 672
            AR  LIN  GI E T    KY+ME+SS  Y   W F  QALPA+L  RG+A EDP A H
Sbjct: 567 LARQILINGGGIFEITVFPSKYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPH 626

Query: 673 GLKLNIKDYPFANDGLILWDALKEWVTEYVNHYYPDPYLITSDTELQAWWTEIRTVGHAD 732
           GL+L IKDYP+A DGL +W A++ WV +Y+  +Y     I +DTELQAWW E+R  GH D
Sbjct: 627 GLRLRIKDYPYAVDGLEVWYAIESWVRDYIFLFYKIEEDIQTDTELQAWWKEVREEGHGD 686

Query: 733 KKYAPGWPALNTPQDLIDIITNIAWVASAHHAAVNFGQYAYAGYFPNRPSIARTNMPTED 792
           KK  P WP + T ++L++  T I WVASA HAAVNFGQY  AGY PNRP+I+R  MP E 
Sbjct: 687 KKSEPWWPKMQTREELVESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKE- 746

Query: 793 FNPALWRSFLEKPEDVLLDAFPSPNQATTVMLVLDVLSSHSPDEEYIGKTMEPSWGDDPV 852
            N   +    + P+ V L    +  Q    + ++++LS+HS DE Y+G+     W  +  
Sbjct: 747 -NTPEFEELEKNPDKVFLKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKE 806

Query: 853 IKAAFDRFSMKMKELEVIIDYRNADCNLKNRTGAGITPYELLKPFSGPGVTGKGVPCSVS 899
              AF++F  K+KE+E  ID RN D  LKNRTG    PY LL P S  GVTG+G+P SVS
Sbjct: 807 ALEAFEKFGEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVS 859