MS010224 (gene) Bitter gourd (TR) v1

BLAST of MS010224 vs. NCBI nr
Match: XP_022958149.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1385.5 bits (3585), Expect = 0.0e+00
Identity = 665/865 (76.88%), Postives = 741/865 (85.66%), Query Frame = 0

Query: 1   SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEIL 60
           SV+KAS  A  + P+SAKAVVTV RP+       +NFG+K+RL DIT LWG KTLVLE+L
Sbjct: 39  SVVKASVDAPMDHPLSAKAVVTVHRPLRG---LSLNFGIKQRLHDITDLWGAKTLVLELL 98

Query: 61  SAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFE 120
           SAEID                       + TGLEKGTIKGY ++V+++ D+VI YE+KF+
Sbjct: 99  SAEID-----------------------SRTGLEKGTIKGYAERVDRDGDDVITYETKFK 158

Query: 121 VPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTK 180
           VPAEFG+IGAVLVEN+HN+ELFL+DIV+ G+PT PLRFSCNSWL  KSQSDD RIFFTTK
Sbjct: 159 VPAEFGSIGAVLVENEHNQELFLKDIVVYGIPTAPLRFSCNSWLTPKSQSDDTRIFFTTK 218

Query: 181 SYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLG 240
           SYLPS TP GLKR RAEEL+ LQGNGYGKRE HHRIYDYDVYNDLG+ DKG EF+RPVLG
Sbjct: 219 SYLPSNTPKGLKRIRAEELKKLQGNGYGKRETHHRIYDYDVYNDLGDPDKGVEFQRPVLG 278

Query: 241 GKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKP 300
           GKQ+PYPRRCRTGRAR ++D LSEVRS D+YLPRDEAF RI+ V LL  TFYS++N LK 
Sbjct: 279 GKQHPYPRRCRTGRARCQTDSLSEVRSWDIYLPRDEAFSRIEYVRLLGNTFYSLLNSLK- 338

Query: 301 ALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKD 360
            LE  + DTK  +    +  +   KG WN N +LPKL   VV K VDIL L SSE F KD
Sbjct: 339 -LETTITDTKFSITEELSNSNKGIKGIWNFNAILPKLFSSVVTKAVDILTLPSSETFDKD 398

Query: 361 KFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIM 420
           KFFWFRDEEFARQ LAGLNPYSIRLVKEWPLKS+LEP IYG PESAITDEIVE+QIKG+M
Sbjct: 399 KFFWFRDEEFARQTLAGLNPYSIRLVKEWPLKSQLEPDIYGPPESAITDEIVERQIKGVM 458

Query: 421 TVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAP 480
           T+ EAV+Q KLYILDYHDL +PYVAKVRKLKGKTLYGSRTLFFLNPD TLRPLAIELS P
Sbjct: 459 TLEEAVKQKKLYILDYHDLLVPYVAKVRKLKGKTLYGSRTLFFLNPDGTLRPLAIELSRP 518

Query: 481 PIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAH 540
           P+DG+ QW+DV TPCWDATGLWLW+I KAHVLAHD+GHHQLVSHWLRTHC VEPYIIA H
Sbjct: 519 PMDGKAQWKDVLTPCWDATGLWLWKIGKAHVLAHDSGHHQLVSHWLRTHCCVEPYIIATH 578

Query: 541 RQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQW 600
           RQLSAMHPIYRLLHPHFRYTMETNAIAR +LINEGGIIETTFSPGKYSMEFSS+AY + W
Sbjct: 579 RQLSAMHPIYRLLHPHFRYTMETNAIARSSLINEGGIIETTFSPGKYSMEFSSLAYRQHW 638

Query: 601 QFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYP 660
           QFNLEALPADLIHRGLAVEDPNAPHGLKL+IEDYPYANDGLILWDAIKEWA EYVNHYYP
Sbjct: 639 QFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPYANDGLILWDAIKEWAMEYVNHYYP 698

Query: 661 DPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVN 720
           DPSVVKSD+ELQAWWTEIR VGHGDKK+EPWW VL+TP+DLID+VTTIMW+T+GHHAV N
Sbjct: 699 DPSVVKSDEELQAWWTEIREVGHGDKKEEPWWPVLDTPKDLIDIVTTIMWITTGHHAVAN 758

Query: 721 FGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSSVLNI 780
           FGQYSFAKYFPNRPSIAR N+PTE+P K WKYFLEKPENVFL+TFPTQ QATIVSSV ++
Sbjct: 759 FGQYSFAKYFPNRPSIARANVPTEQPKKHWKYFLEKPENVFLETFPTQIQATIVSSVASV 818

Query: 781 LSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGV 840
           L+SH  DEKYLGKD+E AWADEP+I +AF KFS KL+KLE+IIDERN NP+LKNRHGAG+
Sbjct: 819 LASHPPDEKYLGKDMEAAWADEPNINQAFNKFSEKLRKLEKIIDERNVNPELKNRHGAGI 875

Query: 841 EPYQLLKPYSEPGVTGKGVPYSISI 866
           EPY+LLKP+SEPGVTGKGVP+SISI
Sbjct: 879 EPYELLKPHSEPGVTGKGVPFSISI 875

BLAST of MS010224 vs. NCBI nr
Match: XP_022148734.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1299.6 bits (3362), Expect = 0.0e+00
Identity = 636/868 (73.27%), Postives = 718/868 (82.72%), Query Frame = 0

Query: 1   SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL--VLE 60
           S +K+SA      PVSA  VVT+KRP      FP        +  ITH W TKT   VLE
Sbjct: 54  SAVKSSAATPTGNPVSAAVVVTLKRPP-----FP-------SIDGITHFWATKTKPPVLE 113

Query: 61  ILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESK 120
           +LSA++DP+                       TGLEKGTIK Y +KV +ED EVIMY+ K
Sbjct: 114 LLSADLDPT-----------------------TGLEKGTIKVYAKKVAREDAEVIMYDIK 173

Query: 121 FEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFT 180
           F +PA+FGAIGAVLVENQHN+ELFL+DIVIQGLPTGP+RFSCNSWL+ KS S DRRIFFT
Sbjct: 174 FVIPADFGAIGAVLVENQHNKELFLKDIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFT 233

Query: 181 TKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPV 240
           TKSYLPS TP GLKR RA+ELRNLQ NG GKREAHHRIYDYDVYNDLG+ D GDE+KRPV
Sbjct: 234 TKSYLPSNTPDGLKRFRAKELRNLQDNGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPV 293

Query: 241 LGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGL 300
           LGGKQ+PYPRRCRTGR+R  +DPLSEVRSL+VY+PRDE FL IKQV +LAK    M++ +
Sbjct: 294 LGGKQHPYPRRCRTGRSRSPTDPLSEVRSLNVYVPRDETFLEIKQVNMLAKAVSWMLHCV 353

Query: 301 KPALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFG 360
            P LEA   DT L   HLS T+++ +K TWNLNTLL +L+DFV  K VD+LP + S+ F 
Sbjct: 354 VPTLEA---DTNLCSSHLSNTNNL-SKHTWNLNTLLRRLVDFVAHKVVDVLP-SPSQTFN 413

Query: 361 KDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKG 420
            DKFFWFRDEEFARQ LAGLNPYSIRLV EWP+KSKL+P IYG PESAITDEIVE+QIKG
Sbjct: 414 GDKFFWFRDEEFARQTLAGLNPYSIRLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKG 473

Query: 421 IMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELS 480
            MT++EAV+Q KL+ LDYHDLFLPYV KVRKLKGKTLYGSRTLFFLNPDNTL+PLAIEL+
Sbjct: 474 FMTLDEAVKQKKLFALDYHDLFLPYVTKVRKLKGKTLYGSRTLFFLNPDNTLKPLAIELT 533

Query: 481 APPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIA 540
            PP + +PQW+DVF PC DATGLWLWRIAKAHV+AHD G+HQLVSHWLRTHCSVEPYIIA
Sbjct: 534 RPPTNAKPQWKDVFKPCCDATGLWLWRIAKAHVVAHDFGYHQLVSHWLRTHCSVEPYIIA 593

Query: 541 AHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAK 600
            HRQLSAMHPIYRLLHPHFR T+E NA AR+TLIN  GIIET FSPGKYSME +SV Y K
Sbjct: 594 THRQLSAMHPIYRLLHPHFRGTIEINAAARKTLINVEGIIETAFSPGKYSMEITSVVYQK 653

Query: 601 QWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHY 660
           QWQFNLEALPADLI+RGLAVEDPNAPHGLKLTIEDYP+AND LILW+AIK+WATEYVNHY
Sbjct: 654 QWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDALILWEAIKQWATEYVNHY 713

Query: 661 YPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAV 720
           YP+P+++K D+ELQAWW EIRTVGHGDKKDEPWW +LNTP+DLIDVVTTIMWVTSGHHA 
Sbjct: 714 YPEPNLIKLDEELQAWWMEIRTVGHGDKKDEPWWPILNTPKDLIDVVTTIMWVTSGHHAA 773

Query: 721 VNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSV 780
           VNFGQY F +YFP RPS ARV++PTE+P  +LWKYFLE PE VF DTFPT  +A+++  +
Sbjct: 774 VNFGQYFFTRYFPIRPSFARVDVPTEDPNSRLWKYFLENPEKVFSDTFPTHVEASVLLCI 833

Query: 781 LNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHG 840
           LNILS+H  DEKYLG ++EPAW ++  IK AFEKFSGKLKKLEEIIDERN NPKLKNRHG
Sbjct: 834 LNILSTHPPDEKYLGGEMEPAWGEDAIIKEAFEKFSGKLKKLEEIIDERNGNPKLKNRHG 881

Query: 841 AGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           AGVEPYQLLKPYSEPGVTGKGVPYS+SI
Sbjct: 894 AGVEPYQLLKPYSEPGVTGKGVPYSVSI 881

BLAST of MS010224 vs. NCBI nr
Match: XP_022148713.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 630/859 (73.34%), Postives = 716/859 (83.35%), Query Frame = 0

Query: 14  PVSAKAVVTVKRPVGNGELFPINFGLKRRLPDIT-HLWGTKTLVLEILSAEIDPSASPLF 73
           P SA  VVTVKR V  G L   N GLKR L D+T +LWGTKTLV E+LS E+DP+     
Sbjct: 4   PFSAHVVVTVKRHV--GRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPT----- 63

Query: 74  SSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNK-EDDEVIMYESKF-EVPAEFGAIGA 133
                             TGLEKGTIKGY QKV + E+D  I YE++   +PAEFGAIGA
Sbjct: 64  ------------------TGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGA 123

Query: 134 VLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTKSYLPSKTPGG 193
           VLVENQ+N ELFL+DIVI G+P GPLRFSCNSWL+ KS S DRRIFFTTKSYLPS TP G
Sbjct: 124 VLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEG 183

Query: 194 LKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRC 253
           LKR R E+L+ LQGNG+GKRE+HHRIYDYDVYNDLG+ D+GDEFKRP+LGGKQ+PYPRRC
Sbjct: 184 LKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRC 243

Query: 254 RTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLA-KTFYSMINGLKPALEAIVPDT 313
           RTGRAR ++DPLSEV S + Y+PRDEAF+  KQVT  A KT YSM++G  PALEAI  D 
Sbjct: 244 RTGRARSQADPLSEVTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDI 303

Query: 314 KLHVPHLSTTDDVP-NKGTWNLNTLLPKLIDFVVDKGVDILPLASSEN-FGKDKFFWFRD 373
           +    +      +P +K TW  + LLP+LIDF VDK V IL L  S+     DKFFWF+D
Sbjct: 304 ESRFTNRVML--LPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKD 363

Query: 374 EEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIMTVNEAVE 433
           EEFARQ LAGLNP SIRLV EWPL+SKL+PAIYG  +SAIT+EIVEQQIKG MT+NEAVE
Sbjct: 364 EEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNEAVE 423

Query: 434 QNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQ 493
           Q KLYILDYHDLFLPYVAKVRK++GKTLYGSRTLFFLNPDNTL+PLAIEL+ PPIDG+ Q
Sbjct: 424 QKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQ 483

Query: 494 WRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMH 553
           W+DVFTPC ++TGLWLWRIAKAHVLAHD+G+HQLVSHWLRTHCSVEPYIIA HRQLSAMH
Sbjct: 484 WKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMH 543

Query: 554 PIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEAL 613
           PIYRLLHPHFRYTME NAIAR+ L N GGIIET FS GKYSME SS+ Y KQWQFNLEAL
Sbjct: 544 PIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEAL 603

Query: 614 PADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKS 673
           PADLIHRG+AVEDPNA HG+KL+IEDYP+ANDGLILWDAIKEWATEYVNHYYPDP VVKS
Sbjct: 604 PADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKS 663

Query: 674 DDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFA 733
           D ELQAWWTEIR+VGHGDKK+EPWW +LNTPEDLIDVV+++MWVTSGHHA VNFGQY FA
Sbjct: 664 DKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFA 723

Query: 734 KYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVLNILSSHSS 793
            YFP RPS+AR+N+PTEEP  KLWK FLE PE VFLDTFP   QAT++ S+L+ILS+HS 
Sbjct: 724 SYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSP 783

Query: 794 DEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLL 853
           +E+YLG+D+EPAW D+P +K+AF+KFSG+LKKLE+IID+RNANPKLKNRHGAGV+PYQLL
Sbjct: 784 EEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLL 835

Query: 854 KPYSEPGVTGKGVPYSISI 866
           KPYSEPGVT +GVP S+SI
Sbjct: 844 KPYSEPGVTARGVPCSVSI 835

BLAST of MS010224 vs. NCBI nr
Match: XP_022943468.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 605/875 (69.14%), Postives = 709/875 (81.03%), Query Frame = 0

Query: 1   SVIKASAVATG-NKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEI 60
           +VIKAS+VA+   KP+S KA+VTV+R +G+G        L+R L DI  L+G K+LV+E+
Sbjct: 59  AVIKASSVASSTEKPLSVKALVTVQRTLGSG------LYLERGLDDIADLFG-KSLVVEL 118

Query: 61  LSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKF 120
           +SAE+DP                       ATGLEK TI+ Y  K + E DE I YE+ F
Sbjct: 119 VSAEVDP-----------------------ATGLEKATIRHYAHKEDTEGDE-ITYEADF 178

Query: 121 EVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT 180
           ++P +FG IGA+ VEN+H++E++++DIV++G P GPL F C+SW+N K   D +R+FFTT
Sbjct: 179 KIPPDFGIIGAIFVENEHHKEMYVKDIVLEGHPIGPLHFVCSSWVNEKDLPDSKRVFFTT 238

Query: 181 KSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVL 240
           KSYLPS TP GLK+ R EEL+ ++GNG GKRE+H RIYDYDVYNDLG+ +K  E KRPVL
Sbjct: 239 KSYLPSNTPEGLKKLREEELKAVRGNGVGKRESHERIYDYDVYNDLGDPNKNPEHKRPVL 298

Query: 241 GGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLK 300
           GGKQ+PYPRRCRTGR R   DP+SE RSL+ Y+PRDEAF  +KQ T  A +  S++NGL 
Sbjct: 299 GGKQFPYPRRCRTGRPRASRDPVSESRSLNNYVPRDEAFSALKQATFNASSLNSVLNGLI 358

Query: 301 PALEAIVPDTKLHVPHLSTTDDV--------PNKGTWNLNTLLPKLIDFVVDKGVDILPL 360
           PALE++  D  +  PH    DD+        P+KG W L TLLP++ID VVD   DIL  
Sbjct: 359 PALESVSTDNDIRFPHFPAIDDLFDNGVPLPPSKGGWKLATLLPRIIDTVVDSAEDILRF 418

Query: 361 ASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEI 420
              E F +DKFFWFRDEEF RQ LAGLNPYS+RLV EWPLKSKL+PA+YG PES IT +I
Sbjct: 419 HPPETFDRDKFFWFRDEEFGRQTLAGLNPYSLRLVTEWPLKSKLDPAVYGPPESEITGKI 478

Query: 421 VEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLR 480
           +EQQI+G MT++EA+ + +L+ILDYHDL LPYVAKVRKLKG TLYGSRTLFFL+ D+TL+
Sbjct: 479 IEQQIRGFMTLDEALTKKRLFILDYHDLLLPYVAKVRKLKGTTLYGSRTLFFLHSDSTLK 538

Query: 481 PLAIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCS 540
           PLAIEL+ PPIDG+PQW+ VFTP WDAT +WLWRIAKAHVLAHD+G+HQLVSHWLRTHC 
Sbjct: 539 PLAIELTRPPIDGKPQWKKVFTPFWDATSVWLWRIAKAHVLAHDSGYHQLVSHWLRTHCC 598

Query: 541 VEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEF 600
           VEPYIIA++RQLSAMHPIYRLLHPHFRYTME NA+ARQ LIN  GIIET+FSPGKYSMEF
Sbjct: 599 VEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPGKYSMEF 658

Query: 601 SSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWA 660
           SSVAY +QWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYP+ANDGLILWDAIK+WA
Sbjct: 659 SSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWA 718

Query: 661 TEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWV 720
           TEYVN+YYPDPS+VKSD+ELQAWWTEIRT GH DKKDEPWW VLNTPEDLI VVTTIMWV
Sbjct: 719 TEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWV 778

Query: 721 TSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMK-LWKYFLEKPENVFLDTFPTQTQ 780
           TSGHHA VNFGQYSFA YFPNRPSIAR+N+P EE  K  W+YFL KPE+V L+TFPTQ Q
Sbjct: 779 TSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQ 838

Query: 781 ATIVSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANP 840
           AT V++VLNILSSHS DE+Y+GKD+E AWADEP IK AFEKFSG+LK+LE IID+RNA+P
Sbjct: 839 ATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIIDKRNADP 898

Query: 841 KLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
            LKNRHGAGV PY+LLKP SEPGVTGKGVPYSISI
Sbjct: 899 NLKNRHGAGVTPYELLKPESEPGVTGKGVPYSISI 902

BLAST of MS010224 vs. NCBI nr
Match: XP_022958530.1 (linoleate 13S-lipoxygenase 2-1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 618/872 (70.87%), Postives = 701/872 (80.39%), Query Frame = 0

Query: 1   SVIKASAVATG----NKPVSAKAVV-TVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL 60
           +VIK SAV         PV  KAVV TV+RP+   +LFP  FG   RL DI +   ++ L
Sbjct: 53  NVIKPSAVHVSVPSIQSPVYTKAVVTTVQRPI--HQLFP-KFGFIGRLHDIINR-RSRVL 112

Query: 61  VLEILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMY 120
            LE+LSA++DP+                       TGLEKG +K Y QKV +EDDE+I Y
Sbjct: 113 ALELLSADMDPT-----------------------TGLEKGRMKAYEQKVKREDDEII-Y 172

Query: 121 ESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRI 180
           E  F +PA+FG IGAVLVEN H +E+FL+DIVI G+PTGPL FSCNSW+ SK+Q +DRRI
Sbjct: 173 EISFVIPADFGPIGAVLVENMHKKEVFLKDIVIHGIPTGPLHFSCNSWITSKAQCNDRRI 232

Query: 181 FFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFK 240
           FFTTKSYLPSKTP GLKR R EELR LQGNGYGKRE   RIYDYDVYNDLGN DKGDE+K
Sbjct: 233 FFTTKSYLPSKTPDGLKRLRLEELRILQGNGYGKREKQERIYDYDVYNDLGNPDKGDEYK 292

Query: 241 RPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAF-LRIKQVTLLAKTFYSM 300
           RPVLGGKQ+PYPRRCRTGR R ++DP SEV+SL+ Y+PRDEAF + + Q   L K  +SM
Sbjct: 293 RPVLGGKQHPYPRRCRTGRPRTQTDPSSEVKSLEFYVPRDEAFSIPMIQENPLTKALHSM 352

Query: 301 INGLKPALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASS 360
           ++ L PAL+ I  D +  +          +K TW  N+LLP L+      G+ +L  + +
Sbjct: 353 LSALLPALDTIGTDKQCSL----------SKHTWRFNSLLPTLM------GILLLDSSQT 412

Query: 361 ENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQ 420
            N G DKFFWF+DEEFARQ LAGLNPYSIRLV EWPL+SKLEP+IYGSPESAITDEIVEQ
Sbjct: 413 SNNG-DKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLESKLEPSIYGSPESAITDEIVEQ 472

Query: 421 QIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLA 480
           QIKG MT++EAV+Q KL+ILDYHDLF+PYVAKVR+LKG+TLYGSRTLFFLNPDNTLRPLA
Sbjct: 473 QIKGFMTIDEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLA 532

Query: 481 IELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEP 540
           IELS PPID +PQW+DVFTPCWDA GLWLWRIAKAHVLAHD+GHHQLVSHWLRTHC VEP
Sbjct: 533 IELSRPPIDDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGHHQLVSHWLRTHCCVEP 592

Query: 541 YIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSV 600
           Y+IA HRQLSAMHPIYRLLHPHFR+TM  NA+AR+TLIN  G++E+ FSPGKYSME SSV
Sbjct: 593 YVIATHRQLSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLVESIFSPGKYSMEISSV 652

Query: 601 AYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEY 660
            Y KQWQFNLEALPADLIHRGLAVEDPNAPHGLKL+IEDYP+ANDGLILWDAIK+W TEY
Sbjct: 653 VYEKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEY 712

Query: 661 VNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSG 720
           VNHYYPDPS+V SD ELQAWWTEIR VGHGDKKDEPWW +LNTP DLI +VTTI+WVTSG
Sbjct: 713 VNHYYPDPSLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSG 772

Query: 721 HHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATI 780
           HHA VNFGQY F  Y+P RPS+ R+N+PTEEP   L KYFLE PENVFLDTFPTQ QA I
Sbjct: 773 HHAAVNFGQYPFTSYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFLDTFPTQIQANI 832

Query: 781 VSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLK 840
           +  +LNILSSHS DE+YLG D+EPAW DEP IK AFEKFS KLKKLEEIIDERN NPKLK
Sbjct: 833 LLFILNILSSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLK 879

Query: 841 NRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           NRHGAGV PYQ+LKPYSEPGVT +GVP S+SI
Sbjct: 893 NRHGAGVGPYQILKPYSEPGVTARGVPCSVSI 879

BLAST of MS010224 vs. ExPASy Swiss-Prot
Match: O24370 (Linoleate 13S-lipoxygenase 2-1, chloroplastic OS=Solanum tuberosum OX=4113 GN=LOX2.1 PE=1 SV=1)

HSP 1 Score: 1042.3 bits (2694), Expect = 3.0e-303
Identity = 500/872 (57.34%), Postives = 640/872 (73.39%), Query Frame = 0

Query: 6   SAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEILSAEID 65
           + +++  K    KAVVTV++ V        N  L R L DI  L G K+L+L I++AE+D
Sbjct: 61  AVLSSTEKATGVKAVVTVQKQV--------NLNLSRGLDDIGDLLG-KSLLLWIVAAELD 120

Query: 66  PSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFEVPAEF 125
                                    TG+EK  I+ Y  +  ++ D    YE+ F +P +F
Sbjct: 121 HK-----------------------TGIEKPGIRAYAHR-GRDVDGDTHYEADFVIPQDF 180

Query: 126 GAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTKSYLPS 185
           G +GA+L+EN+H++E+++++IVI G   G +  +CNSW++SK  + D+RIFFT KSYLPS
Sbjct: 181 GEVGAILIENEHHKEMYVKNIVIDGFVHGKVEITCNSWVHSKFDNPDKRIFFTNKSYLPS 240

Query: 186 KTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSD-KGDEFKRPVLGGKQY 245
           +TP G+ R R EEL  L+G+G G+R+   RIYDYDVYNDLG +D   D+ KRPVLGGK+ 
Sbjct: 241 QTPSGVSRLREEELVTLRGDGIGERKVFERIYDYDVYNDLGEADSNNDDAKRPVLGGKEL 300

Query: 246 PYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALEA 305
           PYPRRC+TGR R + DPLSE RS  VY+PRDEAF  +K V     T YS+++ + PALE+
Sbjct: 301 PYPRRCKTGRPRSKKDPLSETRSTFVYVPRDEAFSEVKSVAFSGNTVYSVLHAVVPALES 360

Query: 306 IVPDTKLHVPHLSTTDDVPNKGT---------WNLNTLLPKLIDFVVDKGVDILPLASSE 365
           +V D  L  PH    D + N G            L  ++P+LI  + D   D+L   S +
Sbjct: 361 VVTDPNLGFPHFPAIDSLFNVGVDLPGLGDKKSGLFNVVPRLIKAISDTRKDVLLFESPQ 420

Query: 366 NFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQ 425
              +DKF WFRD EFARQ LAGLNPYSIRLV EWPL+SKL+P +YG PES IT E++E++
Sbjct: 421 LVQRDKFSWFRDVEFARQTLAGLNPYSIRLVTEWPLRSKLDPKVYGPPESEITKELIEKE 480

Query: 426 IKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAI 485
           I   MTV +AV+Q KL+ILDYHDL LPYV KV +LKG  LYGSRT+FFL P  TL+PLAI
Sbjct: 481 IGNYMTVEQAVQQKKLFILDYHDLLLPYVNKVNELKGSMLYGSRTIFFLTPQGTLKPLAI 540

Query: 486 ELSAPPIDGQPQWRDVFTP-CWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEP 545
           EL+ PP+D +PQW++V++P  W+ATG WLW++AKAHVL+HD+G+HQLVSHWLRTHC  EP
Sbjct: 541 ELTRPPVDDKPQWKEVYSPNDWNATGAWLWKLAKAHVLSHDSGYHQLVSHWLRTHCCTEP 600

Query: 546 YIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSV 605
           YIIA++RQLSAMHPIYRLLHPHFRYTME NA+AR+ LIN  G+IE++F PGKY++E SS+
Sbjct: 601 YIIASNRQLSAMHPIYRLLHPHFRYTMEINALAREALINANGVIESSFFPGKYAIELSSI 660

Query: 606 AYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEY 665
           AY  +W+F+ EALP +LI RGLAVEDPN PHGLKL IEDYP+ANDGL+LWD +K+W T Y
Sbjct: 661 AYGAEWRFDQEALPQNLISRGLAVEDPNEPHGLKLAIEDYPFANDGLVLWDILKQWVTNY 720

Query: 666 VNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSG 725
           VNHYYP  ++++SD ELQAWW+EI+ VGHGDK+DEPWW  L TP DLI ++TTI+WVTSG
Sbjct: 721 VNHYYPQTNLIESDKELQAWWSEIKNVGHGDKRDEPWWPELKTPNDLIGIITTIVWVTSG 780

Query: 726 HHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATI 785
           HHA VNFGQYS+A YFPNRP++AR  MPTE+P  + W++F+ KPE   L  FP+Q QAT 
Sbjct: 781 HHAAVNFGQYSYAGYFPNRPTVARSKMPTEDPTAEEWEWFMNKPEEALLRCFPSQIQATK 840

Query: 786 VSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLK 845
           V ++L++LS+HS DE+Y+G+ IEP WA++P I  AFE FSGKLK+LE IID RN + KL 
Sbjct: 841 VMAILDVLSNHSPDEEYIGEKIEPYWAEDPVINAAFEVFSGKLKELEGIIDARNNDSKLS 899

Query: 846 NRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           NR+GAGV PY+LLKPYSEPGVTGKGVPYSISI
Sbjct: 901 NRNGAGVMPYELLKPYSEPGVTGKGVPYSISI 899

BLAST of MS010224 vs. ExPASy Swiss-Prot
Match: R9WS04 (Lipoxygenase 2, chloroplastic OS=Tanacetum cinerariifolium OX=118510 GN=LOX2 PE=2 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 9.0e-284
Identity = 485/879 (55.18%), Postives = 628/879 (71.44%), Query Frame = 0

Query: 1   SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEIL 60
           S IKA    +  K +  K VVTV +P  +G L  +  GL   + D    +  ++ +LE++
Sbjct: 54  SNIKAIMPDSIGKSIRKKCVVTV-QPTISGALTAVTVGLLGTVADSVSDFLGRSFLLELV 113

Query: 61  SAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYG--QKVNKEDDEVIMYESK 120
           S+++D S                        G EK T+K Y    +++KE  ++  Y+ +
Sbjct: 114 SSDLDSS------------------------GKEKDTVKAYATYDELDKE-SKLYKYQCE 173

Query: 121 FEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFT 180
           FEVP +FG IGAVLV+N+ + + ++++IV+  + T    F+C+SW++SK  + D+RIFF 
Sbjct: 174 FEVPDDFGEIGAVLVQNERHRDAYVKNIVLDEIVT----FTCDSWIHSKFDNPDKRIFFL 233

Query: 181 TKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPV 240
            KSYLPS+TP GLK  R ++L +L+GNG G+R++  RIYDYD YND+G+ D   +  RPV
Sbjct: 234 NKSYLPSETPEGLKSLRQKDLESLRGNGEGERQSFDRIYDYDTYNDIGDPDTDSDMARPV 293

Query: 241 LGGKQYPYPRRCRTGRARYRSDPLSEVR-SLDVYLPRDEAFLRIKQVTLLAKTFYSMING 300
           LGG ++P+PRRCRTGR    ++P SE R +L  Y+PRDE F  IKQ+T  A T YS+++G
Sbjct: 294 LGGNEHPFPRRCRTGRKMTSTEPWSESRTTLPFYVPRDEDFAEIKQITRGATTLYSVLHG 353

Query: 301 LKPALEAIVPDTKLHVPHLS----------TTDDVPNKGTWNLNTLLPKLIDFVVDKGVD 360
           + PAL +++ D     P               D  P+ GT    + LP+L+  + +    
Sbjct: 354 VIPALSSVLKDEDKGFPLFRDIELLYEKGVDIDPPPDSGTL---SALPRLVKAITNSTKK 413

Query: 361 ILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAI 420
           +L   +     KD F WFRDEEF RQ LAGLNPYSI+LV EWPL SKL+P +YG  ESAI
Sbjct: 414 VLQFETPRIKHKDSFSWFRDEEFCRQTLAGLNPYSIQLVTEWPLMSKLDPEVYGPAESAI 473

Query: 421 TDEIVEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPD 480
           T E VE++IKG MT  EA+EQ +L++LDYHDL LPYV KVR+++G TLYGSRTL FL   
Sbjct: 474 TKETVEEEIKGFMTXEEALEQKRLFLLDYHDLLLPYVNKVREIEGTTLYGSRTLMFLTCT 533

Query: 481 NTLRPLAIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLR 540
            TLRPLAIEL+ PP +G+PQW+ V+TPCWDAT  WLW++AKAHVLAHD+G+HQLVSHWLR
Sbjct: 534 GTLRPLAIELTRPPNNGKPQWKHVYTPCWDATDXWLWKLAKAHVLAHDSGYHQLVSHWLR 593

Query: 541 THCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKY 600
           THC  EPYIIA +RQLS MHPI RLL PH RYTM+ N +AR +LIN  GIIE++FSP KY
Sbjct: 594 THCVTEPYIIATNRQLSKMHPIQRLLCPHLRYTMQINGLARLSLINANGIIESSFSPRKY 653

Query: 601 SMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAI 660
           SM+ SS AYA++W+F+ EALPADLI RG+AVED +APHG+KLTIEDYP+ANDGL+LWDAI
Sbjct: 654 SMQLSSDAYAQKWRFDHEALPADLISRGMAVEDESAPHGIKLTIEDYPFANDGLLLWDAI 713

Query: 661 KEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTT 720
           K+WAT Y+NHYYP   +V+SD+ELQAWWTEIRTVGH DKKDEPWW  L T +DLI VV+T
Sbjct: 714 KQWATAYINHYYPQAKLVESDEELQAWWTEIRTVGHADKKDEPWWPQLKTQQDLIGVVST 773

Query: 721 IMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFP 780
           IMWV+SGHH+ VNFGQY F  YFPNRP+IAR  MP E+P  + W+ F+EKPE+V L+ FP
Sbjct: 774 IMWVSSGHHSAVNFGQYDFGGYFPNRPTIARTKMPNEDPTFEEWEAFMEKPEDVLLNCFP 833

Query: 781 TQTQATIVSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDER 840
           TQ QAT V ++L++LSSHS DE+Y+G  +E +W  EP IK AFE+F G+LKKL++IID R
Sbjct: 834 TQIQATKVMAILDVLSSHSPDEEYIGTSMEASWEAEPAIKSAFEEFCGRLKKLDDIIDSR 893

Query: 841 NANPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           N +P L+NR GAG+  YQLLKP+S  GVTGKGVPYSISI
Sbjct: 894 NRDPILRNRTGAGLVQYQLLKPFSGHGVTGKGVPYSISI 899

BLAST of MS010224 vs. ExPASy Swiss-Prot
Match: P38418 (Lipoxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LOX2 PE=1 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 2.5e-265
Identity = 443/791 (56.01%), Postives = 567/791 (71.68%), Query Frame = 0

Query: 85  LKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFEVPAEFGAIGAVLVENQHNEELFLE 144
           ++L++A   ++ T++ Y Q+V  E  +   YE +FE+P +FG +GA+ ++NQ++ +LFL+
Sbjct: 108 VELISAKTDQRITVEDYAQRVWAEAPDE-KYECEFEMPEDFGPVGAIKIQNQYHRQLFLK 167

Query: 145 DIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTKSYLPSKTPGGLKRSRAEELRNLQG 204
            + ++ LP G + F+C SW+  KS    +RIFF+ KSYLPS+TP  LK+ R EEL  LQG
Sbjct: 168 GVELK-LPGGSITFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQG 227

Query: 205 NG---YGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDP 264
                 G+     RIYDYDVYND+G+ D   E  RPV+GG  +PYPRRC+TGR    +DP
Sbjct: 228 KNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDP 287

Query: 265 LSEVR-SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALEAIVPDTKLHVPHLSTTD 324
            SE R   + Y+PRDE F   K  +   K   + +  + P +E+++   +   PH     
Sbjct: 288 SSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQ 347

Query: 325 DVPNKGTW-----NLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPL 384
           ++  +G        L  LLP++I  + +   DIL   +     +D+F W RD+EFARQ L
Sbjct: 348 NLFEEGIQLPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTL 407

Query: 385 AGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIMTVNEAVEQNKLYILD 444
           AGLNPYSI+LV+EWPL SKL+PA+YG P S IT EIVE+++KG MTV+EA++  +L++LD
Sbjct: 408 AGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLD 467

Query: 445 YHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWRDVFTPC 504
           YHDL LPYV KVR+L   TLY SRTLFFL+ D+TLRP+AIEL+ PP   +PQW+ VFTP 
Sbjct: 468 YHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPG 527

Query: 505 WDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHP 564
           +DAT  WLW +AK H ++HD G+HQL+SHWLRTH   EPYIIAA+RQLSAMHPIYRLLHP
Sbjct: 528 YDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHP 587

Query: 565 HFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRG 624
           HFRYTME NA ARQ+L+N GGIIET F PGKY++E SS  Y K W+F+ E LPADLI RG
Sbjct: 588 HFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRG 647

Query: 625 LAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWW 684
           LA ED  A HG++LTI DYP+ANDGLILWDAIKEW T+YV HYYPD  ++ SD+ELQ WW
Sbjct: 648 LAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWW 707

Query: 685 TEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPS 744
           +E+R +GHGDKKDEPWW VL T +DLI VVTTI WVTSGHHA VNFGQY +  YFPNRP+
Sbjct: 708 SEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPT 767

Query: 745 IARVNMPTEEPM-KLWKYFLEKPENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYLGKD 804
             R+ MPTE+P  +  K F E PE V L T+P+Q QAT+V   L++LS+HS DE+Y+G+ 
Sbjct: 768 TTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQ 827

Query: 805 IEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGV 864
            E +WA+EP I  AFE+F GKL+ LE +IDERN N  LKNR GAGV  Y+LLKP SE GV
Sbjct: 828 QEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGV 887

Query: 865 TGKGVPYSISI 866
           TG GVPYSISI
Sbjct: 888 TGMGVPYSISI 896

BLAST of MS010224 vs. ExPASy Swiss-Prot
Match: P38419 (Lipoxygenase 7, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX1 PE=2 SV=2)

HSP 1 Score: 898.7 bits (2321), Expect = 5.3e-260
Identity = 456/874 (52.17%), Postives = 591/874 (67.62%), Query Frame = 0

Query: 15  VSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEILSAEIDPSASPLFSS 74
           V  KAV T+K  VG          L  R  DI  L G ++L LE++S+E+D         
Sbjct: 84  VRVKAVATIKVTVGE---------LINRSIDIRDLIG-RSLSLELVSSELD--------- 143

Query: 75  PYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFEVPAEFGAIGAVLVE 134
                           TG EK T++ Y   V+ +D  V+ YE+ F+VP+ FG IGA++V 
Sbjct: 144 --------------AKTGKEKATVRSYAHNVDDDDHSVVTYEADFDVPSGFGPIGAIIVT 203

Query: 135 NQHNEELFLEDIVIQGLP----TGPLRFSCNSWLNSKSQSDD----RRIFFTTKSYLPSK 194
           N+  +E+FLEDI +        +  L   CNSW+  KS  D+    +RIFF  K+YLP +
Sbjct: 204 NELRQEMFLEDINLTASDGAGNSTVLPIRCNSWVQPKSVGDEGTPSKRIFFANKTYLPGQ 263

Query: 195 TPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGG-KQYP 254
           TP GL+  R  +L+  +G+G G+REA  R+YDYDVYNDLGN D   +  RPVLGG KQ+P
Sbjct: 264 TPAGLRSYRKNDLQQKRGDGTGEREADDRVYDYDVYNDLGNPDSNGDLARPVLGGNKQFP 323

Query: 255 YPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALEAI 314
           YPRRCRTGR   + DP SE R  +VY+PRDE F   K+   L KT  S++    PA +++
Sbjct: 324 YPRRCRTGRPPSKKDPKSETRKGNVYVPRDEEFSPEKEDYFLRKTVGSVLQAAVPAAQSL 383

Query: 315 VPD-TKLHVPHLS-------TTDDVPNKGTWNLN---TLLPKLIDFVVDKGVD-ILPLAS 374
           + D  K ++P  S         D V   G   LN   +++P+L++ + D   + IL   +
Sbjct: 384 LLDKLKWNLPFPSFFVIDKLFEDGVELPGVDKLNFLESVVPRLLEHLRDTPAEKILRFET 443

Query: 375 SENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVE 434
             N  KDKF W RDEEFAR+ LAG+NPY+I LV+E+PLKSKL+PA+YG  ESAIT +++E
Sbjct: 444 PANIQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLE 503

Query: 435 QQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPL 494
           +Q++ +MTV EA+ Q +L++LD+HDLFLPYV K+R L   T+YGSRT+FFL  D TL+ L
Sbjct: 504 EQMRRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLDHTTMYGSRTVFFLTDDGTLQLL 563

Query: 495 AIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVE 554
           AIEL+ P    QPQWR VFTP  DAT  WLWR+AKAHV AHD GHH+L++HWLRTHC+VE
Sbjct: 564 AIELTRPASPSQPQWRQVFTPSTDATMSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVE 623

Query: 555 PYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSS 614
           PYIIAA+RQLS MHPIY+LL PHFRYTM  NA AR  LI+ GGIIE +FSP KYSME SS
Sbjct: 624 PYIIAANRQLSEMHPIYQLLRPHFRYTMRINARARSALISAGGIIERSFSPQKYSMELSS 683

Query: 615 VAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATE 674
           VAY K W+F+ EALPADL+ RG+A EDP A HGLKL IEDYP+ANDGL++WDAIK W   
Sbjct: 684 VAYDKLWRFDTEALPADLVRRGMAEEDPTAEHGLKLAIEDYPFANDGLLIWDAIKTWVQA 743

Query: 675 YVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTS 734
           YV  +YPD   V  D+ELQA+WTE+RT GHGDKKD PWW  L++PE L   +TTI+WV +
Sbjct: 744 YVARFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAA 803

Query: 735 GHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPM--KLWKYFLEKPENVFLDTFPTQTQA 794
            HHA VNFGQY F  YFPNRPSIAR  MP EEP+     + FL+ P+    + FP+Q QA
Sbjct: 804 AHHAAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQA 863

Query: 795 TIVSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPK 854
           T+V +VL++LSSHS+DE+YLG +    W  +  ++ A++ F+ +LK++E +ID RN + K
Sbjct: 864 TVVMAVLDVLSSHSTDEEYLGGEQTRPWNSDAAVQAAYDGFAARLKEIEGVIDGRNKDRK 923

Query: 855 LKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           LKNR GAG+ PYQL+KP+S+ GVTG G+P S SI
Sbjct: 924 LKNRCGAGILPYQLMKPFSDSGVTGMGIPNSTSI 924

BLAST of MS010224 vs. ExPASy Swiss-Prot
Match: Q84YK8 (Probable lipoxygenase 8, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=CM-LOX2 PE=2 SV=1)

HSP 1 Score: 898.3 bits (2320), Expect = 6.9e-260
Identity = 441/811 (54.38%), Postives = 572/811 (70.53%), Query Frame = 0

Query: 78  LYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFEVPAEFGAIGAVLVENQH 137
           L+  +   +L   TG +K T+  Y  KV+ +D  V+ YE+ F+VP  FG IGAV+V N+ 
Sbjct: 132 LFLELVSSELEAKTGKKKATVHSYAHKVDDDDHGVVTYEADFDVPTGFGPIGAVVVTNEL 191

Query: 138 NEELFLEDI-VIQGLPTG---PLRFSCNSWLNSKSQSDD----RRIFFTTKSYLPSKTPG 197
            +E+FLED+ +  G   G    L   CNSW+  KS  D+    +RIFF  K+YLP +TP 
Sbjct: 192 GQEMFLEDLNLTAGDGAGNSTVLPIRCNSWVQPKSSIDEGTPGKRIFF-AKAYLPGQTPA 251

Query: 198 GLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGG-KQYPYPR 257
           GL+  R E+L+  +GNG G+REA  R+YDYDVYNDLGN D   +  RPVLGG KQ+PYPR
Sbjct: 252 GLRSYREEDLKQKRGNGAGQREADDRVYDYDVYNDLGNPDSNGDLARPVLGGSKQFPYPR 311

Query: 258 RCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALE-AIVP 317
           RCRTGR   + DP SE R  +VY+PRDE F  +K    L KT  S+++   PA + A++ 
Sbjct: 312 RCRTGRPPSKKDPKSETRKGNVYVPRDEEFSEVKNAQFLLKTLQSVLHAAVPAAQSALID 371

Query: 318 DTKLHVPHLS-------TTDDVPNKGTWN---LNTLLPKLIDFVVDK-GVDILPLASSEN 377
           +  L++P  S         D V   G      L++++P+L++ + D  G  IL   +  N
Sbjct: 372 NLSLNLPFPSFFVIDKLFEDGVELPGVEKLGFLHSIVPRLLELLRDSPGDKILLFDTPAN 431

Query: 378 FGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQI 437
             KDKF W RDEEFAR+ LAG+NPY+I LV+E+PLKSKL+PA+YG  ESAIT +++E+Q+
Sbjct: 432 VQKDKFAWLRDEEFARETLAGINPYAIELVREFPLKSKLDPAVYGPAESAITADLLEEQM 491

Query: 438 KGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIE 497
           + +MTV EA+ Q +L++LD+HDLFLPYV K+R LK  T+YGSRT+FFL  D TLR LAIE
Sbjct: 492 RRVMTVEEAISQKRLFMLDFHDLFLPYVHKIRSLKHTTMYGSRTIFFLTDDGTLRLLAIE 551

Query: 498 LSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYI 557
           L+ P    QPQWR VFTP  D T  WLWR+AKAHV AHD GHH+L++HWLRTHC+VEPYI
Sbjct: 552 LTRPASPSQPQWRQVFTPSTDTTKSWLWRMAKAHVRAHDAGHHELITHWLRTHCAVEPYI 611

Query: 558 IAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAY 617
           IAA+RQLS MHPIY+LLHPHFRYTM  NA+AR  LI+  GIIE +FSP KYSME SSVAY
Sbjct: 612 IAANRQLSEMHPIYQLLHPHFRYTMRINALARSRLISAAGIIELSFSPQKYSMELSSVAY 671

Query: 618 AKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVN 677
            K W+F++EALPADL+ RG+A EDP A HGL+L IEDYP+ANDGL++WDAIK W   YV 
Sbjct: 672 DKLWRFDMEALPADLVRRGMAEEDPTAEHGLRLAIEDYPFANDGLLIWDAIKTWVQAYVA 731

Query: 678 HYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHH 737
            +YPD   V  D+ELQA+WTE+RT GHGDKKD PWW  L++PE L   +TTI+WV + HH
Sbjct: 732 RFYPDADSVAGDEELQAFWTEVRTKGHGDKKDAPWWPKLDSPESLAHTLTTIVWVAAAHH 791

Query: 738 AVVNFGQYSFAKYFPNRPSIARVNMPTEEPM--KLWKYFLEKPENVFLDTFPTQTQATIV 797
           A VNFGQY F  YFPNRPSIAR  MP EEP+     + FL+ P+    + FP+Q QAT+V
Sbjct: 792 AAVNFGQYDFGGYFPNRPSIARTVMPVEEPVDGAAMERFLDNPDQALRECFPSQVQATVV 851

Query: 798 SSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKN 857
            +VL++LS+HS+DE+YLG +    W  +  ++ A+  F+ +LK++E +ID RN + KLKN
Sbjct: 852 MAVLDVLSTHSTDEEYLGGEQTRPWNSDAAVQAAYAGFTARLKEIEGVIDGRNKDRKLKN 911

Query: 858 RHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           R GAG+ PYQL+KP+S+ GVTG G+P S SI
Sbjct: 912 RCGAGILPYQLMKPFSDAGVTGMGIPNSTSI 941

BLAST of MS010224 vs. ExPASy TrEMBL
Match: A0A6J1H492 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459464 PE=3 SV=1)

HSP 1 Score: 1385.5 bits (3585), Expect = 0.0e+00
Identity = 665/865 (76.88%), Postives = 741/865 (85.66%), Query Frame = 0

Query: 1   SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEIL 60
           SV+KAS  A  + P+SAKAVVTV RP+       +NFG+K+RL DIT LWG KTLVLE+L
Sbjct: 39  SVVKASVDAPMDHPLSAKAVVTVHRPLRG---LSLNFGIKQRLHDITDLWGAKTLVLELL 98

Query: 61  SAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFE 120
           SAEID                       + TGLEKGTIKGY ++V+++ D+VI YE+KF+
Sbjct: 99  SAEID-----------------------SRTGLEKGTIKGYAERVDRDGDDVITYETKFK 158

Query: 121 VPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTK 180
           VPAEFG+IGAVLVEN+HN+ELFL+DIV+ G+PT PLRFSCNSWL  KSQSDD RIFFTTK
Sbjct: 159 VPAEFGSIGAVLVENEHNQELFLKDIVVYGIPTAPLRFSCNSWLTPKSQSDDTRIFFTTK 218

Query: 181 SYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLG 240
           SYLPS TP GLKR RAEEL+ LQGNGYGKRE HHRIYDYDVYNDLG+ DKG EF+RPVLG
Sbjct: 219 SYLPSNTPKGLKRIRAEELKKLQGNGYGKRETHHRIYDYDVYNDLGDPDKGVEFQRPVLG 278

Query: 241 GKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKP 300
           GKQ+PYPRRCRTGRAR ++D LSEVRS D+YLPRDEAF RI+ V LL  TFYS++N LK 
Sbjct: 279 GKQHPYPRRCRTGRARCQTDSLSEVRSWDIYLPRDEAFSRIEYVRLLGNTFYSLLNSLK- 338

Query: 301 ALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFGKD 360
            LE  + DTK  +    +  +   KG WN N +LPKL   VV K VDIL L SSE F KD
Sbjct: 339 -LETTITDTKFSITEELSNSNKGIKGIWNFNAILPKLFSSVVTKAVDILTLPSSETFDKD 398

Query: 361 KFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIM 420
           KFFWFRDEEFARQ LAGLNPYSIRLVKEWPLKS+LEP IYG PESAITDEIVE+QIKG+M
Sbjct: 399 KFFWFRDEEFARQTLAGLNPYSIRLVKEWPLKSQLEPDIYGPPESAITDEIVERQIKGVM 458

Query: 421 TVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAP 480
           T+ EAV+Q KLYILDYHDL +PYVAKVRKLKGKTLYGSRTLFFLNPD TLRPLAIELS P
Sbjct: 459 TLEEAVKQKKLYILDYHDLLVPYVAKVRKLKGKTLYGSRTLFFLNPDGTLRPLAIELSRP 518

Query: 481 PIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAH 540
           P+DG+ QW+DV TPCWDATGLWLW+I KAHVLAHD+GHHQLVSHWLRTHC VEPYIIA H
Sbjct: 519 PMDGKAQWKDVLTPCWDATGLWLWKIGKAHVLAHDSGHHQLVSHWLRTHCCVEPYIIATH 578

Query: 541 RQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQW 600
           RQLSAMHPIYRLLHPHFRYTMETNAIAR +LINEGGIIETTFSPGKYSMEFSS+AY + W
Sbjct: 579 RQLSAMHPIYRLLHPHFRYTMETNAIARSSLINEGGIIETTFSPGKYSMEFSSLAYRQHW 638

Query: 601 QFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYP 660
           QFNLEALPADLIHRGLAVEDPNAPHGLKL+IEDYPYANDGLILWDAIKEWA EYVNHYYP
Sbjct: 639 QFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPYANDGLILWDAIKEWAMEYVNHYYP 698

Query: 661 DPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVN 720
           DPSVVKSD+ELQAWWTEIR VGHGDKK+EPWW VL+TP+DLID+VTTIMW+T+GHHAV N
Sbjct: 699 DPSVVKSDEELQAWWTEIREVGHGDKKEEPWWPVLDTPKDLIDIVTTIMWITTGHHAVAN 758

Query: 721 FGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFLEKPENVFLDTFPTQTQATIVSSVLNI 780
           FGQYSFAKYFPNRPSIAR N+PTE+P K WKYFLEKPENVFL+TFPTQ QATIVSSV ++
Sbjct: 759 FGQYSFAKYFPNRPSIARANVPTEQPKKHWKYFLEKPENVFLETFPTQIQATIVSSVASV 818

Query: 781 LSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGV 840
           L+SH  DEKYLGKD+E AWADEP+I +AF KFS KL+KLE+IIDERN NP+LKNRHGAG+
Sbjct: 819 LASHPPDEKYLGKDMEAAWADEPNINQAFNKFSEKLRKLEKIIDERNVNPELKNRHGAGI 875

Query: 841 EPYQLLKPYSEPGVTGKGVPYSISI 866
           EPY+LLKP+SEPGVTGKGVP+SISI
Sbjct: 879 EPYELLKPHSEPGVTGKGVPFSISI 875

BLAST of MS010224 vs. ExPASy TrEMBL
Match: A0A6J1D4V5 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017327 PE=3 SV=1)

HSP 1 Score: 1299.6 bits (3362), Expect = 0.0e+00
Identity = 636/868 (73.27%), Postives = 718/868 (82.72%), Query Frame = 0

Query: 1   SVIKASAVATGNKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL--VLE 60
           S +K+SA      PVSA  VVT+KRP      FP        +  ITH W TKT   VLE
Sbjct: 54  SAVKSSAATPTGNPVSAAVVVTLKRPP-----FP-------SIDGITHFWATKTKPPVLE 113

Query: 61  ILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESK 120
           +LSA++DP+                       TGLEKGTIK Y +KV +ED EVIMY+ K
Sbjct: 114 LLSADLDPT-----------------------TGLEKGTIKVYAKKVAREDAEVIMYDIK 173

Query: 121 FEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFT 180
           F +PA+FGAIGAVLVENQHN+ELFL+DIVIQGLPTGP+RFSCNSWL+ KS S DRRIFFT
Sbjct: 174 FVIPADFGAIGAVLVENQHNKELFLKDIVIQGLPTGPVRFSCNSWLSPKSHSGDRRIFFT 233

Query: 181 TKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPV 240
           TKSYLPS TP GLKR RA+ELRNLQ NG GKREAHHRIYDYDVYNDLG+ D GDE+KRPV
Sbjct: 234 TKSYLPSNTPDGLKRFRAKELRNLQDNGSGKREAHHRIYDYDVYNDLGDPDNGDEYKRPV 293

Query: 241 LGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGL 300
           LGGKQ+PYPRRCRTGR+R  +DPLSEVRSL+VY+PRDE FL IKQV +LAK    M++ +
Sbjct: 294 LGGKQHPYPRRCRTGRSRSPTDPLSEVRSLNVYVPRDETFLEIKQVNMLAKAVSWMLHCV 353

Query: 301 KPALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASSENFG 360
            P LEA   DT L   HLS T+++ +K TWNLNTLL +L+DFV  K VD+LP + S+ F 
Sbjct: 354 VPTLEA---DTNLCSSHLSNTNNL-SKHTWNLNTLLRRLVDFVAHKVVDVLP-SPSQTFN 413

Query: 361 KDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKG 420
            DKFFWFRDEEFARQ LAGLNPYSIRLV EWP+KSKL+P IYG PESAITDEIVE+QIKG
Sbjct: 414 GDKFFWFRDEEFARQTLAGLNPYSIRLVTEWPIKSKLDPCIYGPPESAITDEIVEEQIKG 473

Query: 421 IMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELS 480
            MT++EAV+Q KL+ LDYHDLFLPYV KVRKLKGKTLYGSRTLFFLNPDNTL+PLAIEL+
Sbjct: 474 FMTLDEAVKQKKLFALDYHDLFLPYVTKVRKLKGKTLYGSRTLFFLNPDNTLKPLAIELT 533

Query: 481 APPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIA 540
            PP + +PQW+DVF PC DATGLWLWRIAKAHV+AHD G+HQLVSHWLRTHCSVEPYIIA
Sbjct: 534 RPPTNAKPQWKDVFKPCCDATGLWLWRIAKAHVVAHDFGYHQLVSHWLRTHCSVEPYIIA 593

Query: 541 AHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAK 600
            HRQLSAMHPIYRLLHPHFR T+E NA AR+TLIN  GIIET FSPGKYSME +SV Y K
Sbjct: 594 THRQLSAMHPIYRLLHPHFRGTIEINAAARKTLINVEGIIETAFSPGKYSMEITSVVYQK 653

Query: 601 QWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHY 660
           QWQFNLEALPADLI+RGLAVEDPNAPHGLKLTIEDYP+AND LILW+AIK+WATEYVNHY
Sbjct: 654 QWQFNLEALPADLINRGLAVEDPNAPHGLKLTIEDYPFANDALILWEAIKQWATEYVNHY 713

Query: 661 YPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAV 720
           YP+P+++K D+ELQAWW EIRTVGHGDKKDEPWW +LNTP+DLIDVVTTIMWVTSGHHA 
Sbjct: 714 YPEPNLIKLDEELQAWWMEIRTVGHGDKKDEPWWPILNTPKDLIDVVTTIMWVTSGHHAA 773

Query: 721 VNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSV 780
           VNFGQY F +YFP RPS ARV++PTE+P  +LWKYFLE PE VF DTFPT  +A+++  +
Sbjct: 774 VNFGQYFFTRYFPIRPSFARVDVPTEDPNSRLWKYFLENPEKVFSDTFPTHVEASVLLCI 833

Query: 781 LNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHG 840
           LNILS+H  DEKYLG ++EPAW ++  IK AFEKFSGKLKKLEEIIDERN NPKLKNRHG
Sbjct: 834 LNILSTHPPDEKYLGGEMEPAWGEDAIIKEAFEKFSGKLKKLEEIIDERNGNPKLKNRHG 881

Query: 841 AGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           AGVEPYQLLKPYSEPGVTGKGVPYS+SI
Sbjct: 894 AGVEPYQLLKPYSEPGVTGKGVPYSVSI 881

BLAST of MS010224 vs. ExPASy TrEMBL
Match: A0A6J1D4V6 (Lipoxygenase OS=Momordica charantia OX=3673 GN=LOC111017309 PE=3 SV=1)

HSP 1 Score: 1278.1 bits (3306), Expect = 0.0e+00
Identity = 630/859 (73.34%), Postives = 716/859 (83.35%), Query Frame = 0

Query: 14  PVSAKAVVTVKRPVGNGELFPINFGLKRRLPDIT-HLWGTKTLVLEILSAEIDPSASPLF 73
           P SA  VVTVKR V  G L   N GLKR L D+T +LWGTKTLV E+LS E+DP+     
Sbjct: 4   PFSAHVVVTVKRHV--GRLLLPNLGLKRWLYDVTDYLWGTKTLVFELLSTEMDPT----- 63

Query: 74  SSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNK-EDDEVIMYESKF-EVPAEFGAIGA 133
                             TGLEKGTIKGY QKV + E+D  I YE++   +PAEFGAIGA
Sbjct: 64  ------------------TGLEKGTIKGYAQKVGREEEDGEITYETELVNIPAEFGAIGA 123

Query: 134 VLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTKSYLPSKTPGG 193
           VLVENQ+N ELFL+DIVI G+P GPLRFSCNSWL+ KS S DRRIFFTTKSYLPS TP G
Sbjct: 124 VLVENQYNTELFLKDIVIHGVPYGPLRFSCNSWLSPKSHSHDRRIFFTTKSYLPSNTPEG 183

Query: 194 LKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRC 253
           LKR R E+L+ LQGNG+GKRE+HHRIYDYDVYNDLG+ D+GDEFKRP+LGGKQ+PYPRRC
Sbjct: 184 LKRFREEDLKILQGNGHGKRESHHRIYDYDVYNDLGDPDRGDEFKRPILGGKQHPYPRRC 243

Query: 254 RTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLA-KTFYSMINGLKPALEAIVPDT 313
           RTGRAR ++DPLSEV S + Y+PRDEAF+  KQVT  A KT YSM++G  PALEAI  D 
Sbjct: 244 RTGRARSQADPLSEVTSSNFYVPRDEAFIDTKQVTTPAVKTVYSMLHGFIPALEAIAQDI 303

Query: 314 KLHVPHLSTTDDVP-NKGTWNLNTLLPKLIDFVVDKGVDILPLASSEN-FGKDKFFWFRD 373
           +    +      +P +K TW  + LLP+LIDF VDK V IL L  S+     DKFFWF+D
Sbjct: 304 ESRFTNRVML--LPSSKYTWEPSALLPRLIDFAVDKVVRILLLGPSQAVLDSDKFFWFKD 363

Query: 374 EEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIMTVNEAVE 433
           EEFARQ LAGLNP SIRLV EWPL+SKL+PAIYG  +SAIT+EIVEQQIKG MT+NEAVE
Sbjct: 364 EEFARQTLAGLNPCSIRLVTEWPLESKLDPAIYGPTKSAITNEIVEQQIKGFMTLNEAVE 423

Query: 434 QNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQ 493
           Q KLYILDYHDLFLPYVAKVRK++GKTLYGSRTLFFLNPDNTL+PLAIEL+ PPIDG+ Q
Sbjct: 424 QKKLYILDYHDLFLPYVAKVRKIEGKTLYGSRTLFFLNPDNTLKPLAIELTRPPIDGKQQ 483

Query: 494 WRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMH 553
           W+DVFTPC ++TGLWLWRIAKAHVLAHD+G+HQLVSHWLRTHCSVEPYIIA HRQLSAMH
Sbjct: 484 WKDVFTPCLNSTGLWLWRIAKAHVLAHDSGYHQLVSHWLRTHCSVEPYIIAMHRQLSAMH 543

Query: 554 PIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEAL 613
           PIYRLLHPHFRYTME NAIAR+ L N GGIIET FS GKYSME SS+ Y KQWQFNLEAL
Sbjct: 544 PIYRLLHPHFRYTMEINAIAREALTNVGGIIETAFSLGKYSMEVSSMVYQKQWQFNLEAL 603

Query: 614 PADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKS 673
           PADLIHRG+AVEDPNA HG+KL+IEDYP+ANDGLILWDAIKEWATEYVNHYYPDP VVKS
Sbjct: 604 PADLIHRGMAVEDPNAAHGVKLSIEDYPFANDGLILWDAIKEWATEYVNHYYPDPGVVKS 663

Query: 674 DDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFA 733
           D ELQAWWTEIR+VGHGDKK+EPWW +LNTPEDLIDVV+++MWVTSGHHA VNFGQY FA
Sbjct: 664 DKELQAWWTEIRSVGHGDKKEEPWWPILNTPEDLIDVVSSMMWVTSGHHAAVNFGQYYFA 723

Query: 734 KYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATIVSSVLNILSSHSS 793
            YFP RPS+AR+N+PTEEP  KLWK FLE PE VFLDTFP   QAT++ S+L+ILS+HS 
Sbjct: 724 SYFPARPSVARINVPTEEPNSKLWKRFLEDPERVFLDTFPAHIQATVLLSILSILSTHSP 783

Query: 794 DEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLL 853
           +E+YLG+D+EPAW D+P +K+AF+KFSG+LKKLE+IID+RNANPKLKNRHGAGV+PYQLL
Sbjct: 784 EEEYLGEDMEPAWGDDPVVKQAFDKFSGRLKKLEDIIDQRNANPKLKNRHGAGVDPYQLL 835

Query: 854 KPYSEPGVTGKGVPYSISI 866
           KPYSEPGVT +GVP S+SI
Sbjct: 844 KPYSEPGVTARGVPCSVSI 835

BLAST of MS010224 vs. ExPASy TrEMBL
Match: A0A6J1FWY9 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111448227 PE=3 SV=1)

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 605/875 (69.14%), Postives = 709/875 (81.03%), Query Frame = 0

Query: 1   SVIKASAVATG-NKPVSAKAVVTVKRPVGNGELFPINFGLKRRLPDITHLWGTKTLVLEI 60
           +VIKAS+VA+   KP+S KA+VTV+R +G+G        L+R L DI  L+G K+LV+E+
Sbjct: 59  AVIKASSVASSTEKPLSVKALVTVQRTLGSG------LYLERGLDDIADLFG-KSLVVEL 118

Query: 61  LSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKF 120
           +SAE+DP                       ATGLEK TI+ Y  K + E DE I YE+ F
Sbjct: 119 VSAEVDP-----------------------ATGLEKATIRHYAHKEDTEGDE-ITYEADF 178

Query: 121 EVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTT 180
           ++P +FG IGA+ VEN+H++E++++DIV++G P GPL F C+SW+N K   D +R+FFTT
Sbjct: 179 KIPPDFGIIGAIFVENEHHKEMYVKDIVLEGHPIGPLHFVCSSWVNEKDLPDSKRVFFTT 238

Query: 181 KSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVL 240
           KSYLPS TP GLK+ R EEL+ ++GNG GKRE+H RIYDYDVYNDLG+ +K  E KRPVL
Sbjct: 239 KSYLPSNTPEGLKKLREEELKAVRGNGVGKRESHERIYDYDVYNDLGDPNKNPEHKRPVL 298

Query: 241 GGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLK 300
           GGKQ+PYPRRCRTGR R   DP+SE RSL+ Y+PRDEAF  +KQ T  A +  S++NGL 
Sbjct: 299 GGKQFPYPRRCRTGRPRASRDPVSESRSLNNYVPRDEAFSALKQATFNASSLNSVLNGLI 358

Query: 301 PALEAIVPDTKLHVPHLSTTDDV--------PNKGTWNLNTLLPKLIDFVVDKGVDILPL 360
           PALE++  D  +  PH    DD+        P+KG W L TLLP++ID VVD   DIL  
Sbjct: 359 PALESVSTDNDIRFPHFPAIDDLFDNGVPLPPSKGGWKLATLLPRIIDTVVDSAEDILRF 418

Query: 361 ASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEI 420
              E F +DKFFWFRDEEF RQ LAGLNPYS+RLV EWPLKSKL+PA+YG PES IT +I
Sbjct: 419 HPPETFDRDKFFWFRDEEFGRQTLAGLNPYSLRLVTEWPLKSKLDPAVYGPPESEITGKI 478

Query: 421 VEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLR 480
           +EQQI+G MT++EA+ + +L+ILDYHDL LPYVAKVRKLKG TLYGSRTLFFL+ D+TL+
Sbjct: 479 IEQQIRGFMTLDEALTKKRLFILDYHDLLLPYVAKVRKLKGTTLYGSRTLFFLHSDSTLK 538

Query: 481 PLAIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCS 540
           PLAIEL+ PPIDG+PQW+ VFTP WDAT +WLWRIAKAHVLAHD+G+HQLVSHWLRTHC 
Sbjct: 539 PLAIELTRPPIDGKPQWKKVFTPFWDATSVWLWRIAKAHVLAHDSGYHQLVSHWLRTHCC 598

Query: 541 VEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEF 600
           VEPYIIA++RQLSAMHPIYRLLHPHFRYTME NA+ARQ LIN  GIIET+FSPGKYSMEF
Sbjct: 599 VEPYIIASNRQLSAMHPIYRLLHPHFRYTMEINALARQALINANGIIETSFSPGKYSMEF 658

Query: 601 SSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWA 660
           SSVAY +QWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYP+ANDGLILWDAIK+WA
Sbjct: 659 SSVAYKEQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPFANDGLILWDAIKDWA 718

Query: 661 TEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWV 720
           TEYVN+YYPDPS+VKSD+ELQAWWTEIRT GH DKKDEPWW VLNTPEDLI VVTTIMWV
Sbjct: 719 TEYVNYYYPDPSLVKSDEELQAWWTEIRTEGHADKKDEPWWPVLNTPEDLISVVTTIMWV 778

Query: 721 TSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMK-LWKYFLEKPENVFLDTFPTQTQ 780
           TSGHHA VNFGQYSFA YFPNRPSIAR+N+P EE  K  W+YFL KPE+V L+TFPTQ Q
Sbjct: 779 TSGHHAAVNFGQYSFAGYFPNRPSIARINVPVEEKNKEKWEYFLNKPEDVLLETFPTQLQ 838

Query: 781 ATIVSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANP 840
           AT V++VLNILSSHS DE+Y+GKD+E AWADEP IK AFEKFSG+LK+LE IID+RNA+P
Sbjct: 839 ATKVTAVLNILSSHSPDEEYMGKDMEQAWADEPFIKGAFEKFSGRLKELEGIIDKRNADP 898

Query: 841 KLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
            LKNRHGAGV PY+LLKP SEPGVTGKGVPYSISI
Sbjct: 899 NLKNRHGAGVTPYELLKPESEPGVTGKGVPYSISI 902

BLAST of MS010224 vs. ExPASy TrEMBL
Match: A0A6J1H3S4 (Lipoxygenase OS=Cucurbita moschata OX=3662 GN=LOC111459733 PE=3 SV=1)

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 618/872 (70.87%), Postives = 701/872 (80.39%), Query Frame = 0

Query: 1   SVIKASAVATG----NKPVSAKAVV-TVKRPVGNGELFPINFGLKRRLPDITHLWGTKTL 60
           +VIK SAV         PV  KAVV TV+RP+   +LFP  FG   RL DI +   ++ L
Sbjct: 53  NVIKPSAVHVSVPSIQSPVYTKAVVTTVQRPI--HQLFP-KFGFIGRLHDIINR-RSRVL 112

Query: 61  VLEILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEVIMY 120
            LE+LSA++DP+                       TGLEKG +K Y QKV +EDDE+I Y
Sbjct: 113 ALELLSADMDPT-----------------------TGLEKGRMKAYEQKVKREDDEII-Y 172

Query: 121 ESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDDRRI 180
           E  F +PA+FG IGAVLVEN H +E+FL+DIVI G+PTGPL FSCNSW+ SK+Q +DRRI
Sbjct: 173 EISFVIPADFGPIGAVLVENMHKKEVFLKDIVIHGIPTGPLHFSCNSWITSKAQCNDRRI 232

Query: 181 FFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDEFK 240
           FFTTKSYLPSKTP GLKR R EELR LQGNGYGKRE   RIYDYDVYNDLGN DKGDE+K
Sbjct: 233 FFTTKSYLPSKTPDGLKRLRLEELRILQGNGYGKREKQERIYDYDVYNDLGNPDKGDEYK 292

Query: 241 RPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSLDVYLPRDEAF-LRIKQVTLLAKTFYSM 300
           RPVLGGKQ+PYPRRCRTGR R ++DP SEV+SL+ Y+PRDEAF + + Q   L K  +SM
Sbjct: 293 RPVLGGKQHPYPRRCRTGRPRTQTDPSSEVKSLEFYVPRDEAFSIPMIQENPLTKALHSM 352

Query: 301 INGLKPALEAIVPDTKLHVPHLSTTDDVPNKGTWNLNTLLPKLIDFVVDKGVDILPLASS 360
           ++ L PAL+ I  D +  +          +K TW  N+LLP L+      G+ +L  + +
Sbjct: 353 LSALLPALDTIGTDKQCSL----------SKHTWRFNSLLPTLM------GILLLDSSQT 412

Query: 361 ENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQ 420
            N G DKFFWF+DEEFARQ LAGLNPYSIRLV EWPL+SKLEP+IYGSPESAITDEIVEQ
Sbjct: 413 SNNG-DKFFWFKDEEFARQTLAGLNPYSIRLVTEWPLESKLEPSIYGSPESAITDEIVEQ 472

Query: 421 QIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLA 480
           QIKG MT++EAV+Q KL+ILDYHDLF+PYVAKVR+LKG+TLYGSRTLFFLNPDNTLRPLA
Sbjct: 473 QIKGFMTIDEAVKQRKLFILDYHDLFIPYVAKVRELKGRTLYGSRTLFFLNPDNTLRPLA 532

Query: 481 IELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEP 540
           IELS PPID +PQW+DVFTPCWDA GLWLWRIAKAHVLAHD+GHHQLVSHWLRTHC VEP
Sbjct: 533 IELSRPPIDDKPQWKDVFTPCWDAYGLWLWRIAKAHVLAHDSGHHQLVSHWLRTHCCVEP 592

Query: 541 YIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSV 600
           Y+IA HRQLSAMHPIYRLLHPHFR+TM  NA+AR+TLIN  G++E+ FSPGKYSME SSV
Sbjct: 593 YVIATHRQLSAMHPIYRLLHPHFRHTMAINAVARETLINVEGLVESIFSPGKYSMEISSV 652

Query: 601 AYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEY 660
            Y KQWQFNLEALPADLIHRGLAVEDPNAPHGLKL+IEDYP+ANDGLILWDAIK+W TEY
Sbjct: 653 VYEKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLSIEDYPFANDGLILWDAIKQWVTEY 712

Query: 661 VNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSG 720
           VNHYYPDPS+V SD ELQAWWTEIR VGHGDKKDEPWW +LNTP DLI +VTTI+WVTSG
Sbjct: 713 VNHYYPDPSLVNSDGELQAWWTEIREVGHGDKKDEPWWPILNTPNDLIHIVTTIIWVTSG 772

Query: 721 HHAVVNFGQYSFAKYFPNRPSIARVNMPTEEP-MKLWKYFLEKPENVFLDTFPTQTQATI 780
           HHA VNFGQY F  Y+P RPS+ R+N+PTEEP   L KYFLE PENVFLDTFPTQ QA I
Sbjct: 773 HHAAVNFGQYPFTSYYPIRPSLTRLNIPTEEPNSTLRKYFLENPENVFLDTFPTQIQANI 832

Query: 781 VSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLK 840
           +  +LNILSSHS DE+YLG D+EPAW DEP IK AFEKFS KLKKLEEIIDERN NPKLK
Sbjct: 833 LLFILNILSSHSRDEEYLGADMEPAWGDEPVIKEAFEKFSRKLKKLEEIIDERNGNPKLK 879

Query: 841 NRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           NRHGAGV PYQ+LKPYSEPGVT +GVP S+SI
Sbjct: 893 NRHGAGVGPYQILKPYSEPGVTARGVPCSVSI 879

BLAST of MS010224 vs. TAIR 10
Match: AT3G45140.1 (lipoxygenase 2 )

HSP 1 Score: 916.4 bits (2367), Expect = 1.7e-266
Identity = 443/791 (56.01%), Postives = 567/791 (71.68%), Query Frame = 0

Query: 85  LKLVTATGLEKGTIKGYGQKVNKEDDEVIMYESKFEVPAEFGAIGAVLVENQHNEELFLE 144
           ++L++A   ++ T++ Y Q+V  E  +   YE +FE+P +FG +GA+ ++NQ++ +LFL+
Sbjct: 108 VELISAKTDQRITVEDYAQRVWAEAPDE-KYECEFEMPEDFGPVGAIKIQNQYHRQLFLK 167

Query: 145 DIVIQGLPTGPLRFSCNSWLNSKSQSDDRRIFFTTKSYLPSKTPGGLKRSRAEELRNLQG 204
            + ++ LP G + F+C SW+  KS    +RIFF+ KSYLPS+TP  LK+ R EEL  LQG
Sbjct: 168 GVELK-LPGGSITFTCESWVAPKSVDPTKRIFFSDKSYLPSQTPEPLKKYRKEELETLQG 227

Query: 205 NG---YGKREAHHRIYDYDVYNDLGNSDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDP 264
                 G+     RIYDYDVYND+G+ D   E  RPV+GG  +PYPRRC+TGR    +DP
Sbjct: 228 KNREEVGEFTKFERIYDYDVYNDVGDPDNDPELARPVIGGLTHPYPRRCKTGRKPCETDP 287

Query: 265 LSEVR-SLDVYLPRDEAFLRIKQVTLLAKTFYSMINGLKPALEAIVPDTKLHVPHLSTTD 324
            SE R   + Y+PRDE F   K  +   K   + +  + P +E+++   +   PH     
Sbjct: 288 SSEQRYGGEFYVPRDEEFSTAKGTSFTGKAVLAALPSIFPQIESVLLSPQEPFPHFKAIQ 347

Query: 325 DVPNKGTW-----NLNTLLPKLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPL 384
           ++  +G        L  LLP++I  + +   DIL   +     +D+F W RD+EFARQ L
Sbjct: 348 NLFEEGIQLPKDAGLLPLLPRIIKALGEAQDDILQFDAPVLINRDRFSWLRDDEFARQTL 407

Query: 385 AGLNPYSIRLVKEWPLKSKLEPAIYGSPESAITDEIVEQQIKGIMTVNEAVEQNKLYILD 444
           AGLNPYSI+LV+EWPL SKL+PA+YG P S IT EIVE+++KG MTV+EA++  +L++LD
Sbjct: 408 AGLNPYSIQLVEEWPLISKLDPAVYGDPTSLITWEIVEREVKGNMTVDEALKNKRLFVLD 467

Query: 445 YHDLFLPYVAKVRKLKGKTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWRDVFTPC 504
           YHDL LPYV KVR+L   TLY SRTLFFL+ D+TLRP+AIEL+ PP   +PQW+ VFTP 
Sbjct: 468 YHDLLLPYVNKVRELNNTTLYASRTLFFLSDDSTLRPVAIELTCPPNINKPQWKQVFTPG 527

Query: 505 WDATGLWLWRIAKAHVLAHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHP 564
           +DAT  WLW +AK H ++HD G+HQL+SHWLRTH   EPYIIAA+RQLSAMHPIYRLLHP
Sbjct: 528 YDATSCWLWNLAKTHAISHDAGYHQLISHWLRTHACTEPYIIAANRQLSAMHPIYRLLHP 587

Query: 565 HFRYTMETNAIARQTLINEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRG 624
           HFRYTME NA ARQ+L+N GGIIET F PGKY++E SS  Y K W+F+ E LPADLI RG
Sbjct: 588 HFRYTMEINARARQSLVNGGGIIETCFWPGKYALELSSAVYGKLWRFDQEGLPADLIKRG 647

Query: 625 LAVEDPNAPHGLKLTIEDYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWW 684
           LA ED  A HG++LTI DYP+ANDGLILWDAIKEW T+YV HYYPD  ++ SD+ELQ WW
Sbjct: 648 LAEEDKTAEHGVRLTIPDYPFANDGLILWDAIKEWVTDYVKHYYPDEELITSDEELQGWW 707

Query: 685 TEIRTVGHGDKKDEPWWQVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPS 744
           +E+R +GHGDKKDEPWW VL T +DLI VVTTI WVTSGHHA VNFGQY +  YFPNRP+
Sbjct: 708 SEVRNIGHGDKKDEPWWPVLKTQDDLIGVVTTIAWVTSGHHAAVNFGQYGYGGYFPNRPT 767

Query: 745 IARVNMPTEEPM-KLWKYFLEKPENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYLGKD 804
             R+ MPTE+P  +  K F E PE V L T+P+Q QAT+V   L++LS+HS DE+Y+G+ 
Sbjct: 768 TTRIRMPTEDPTDEALKEFYESPEKVLLKTYPSQKQATLVMVTLDLLSTHSPDEEYIGEQ 827

Query: 805 IEPAWADEPHIKRAFEKFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGV 864
            E +WA+EP I  AFE+F GKL+ LE +IDERN N  LKNR GAGV  Y+LLKP SE GV
Sbjct: 828 QEASWANEPVINAAFERFKGKLQYLEGVIDERNVNITLKNRAGAGVVKYELLKPTSEHGV 887

Query: 865 TGKGVPYSISI 866
           TG GVPYSISI
Sbjct: 888 TGMGVPYSISI 896

BLAST of MS010224 vs. TAIR 10
Match: AT1G17420.1 (lipoxygenase 3 )

HSP 1 Score: 742.7 bits (1916), Expect = 3.4e-214
Identity = 376/831 (45.25%), Postives = 535/831 (64.38%), Query Frame = 0

Query: 53  KTLVLEILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEV 112
           + +VLE++S ++DP                   K           +K + +K +K   E 
Sbjct: 117 RNIVLELISTQLDP-------------------KTKLPKKSNAAVLKDWSKK-SKTKAER 176

Query: 113 IMYESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDD 172
           + Y ++F V A FG+ GA+ V N+H +E FLE I I+G   GP+ F CNSW+ S+    D
Sbjct: 177 VHYTAEFTVDAAFGSPGAITVMNKHQKEFFLESITIEGFALGPVHFPCNSWVQSQKDHPD 236

Query: 173 RRIFFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGD 232
           +RIFFT + YLP++TP GL+  R +EL+NL+G+G G R+   RIYD+DVYNDLGN DK  
Sbjct: 237 KRIFFTNQPYLPNETPSGLRVLREKELKNLRGDGSGVRKLSDRIYDFDVYNDLGNPDKSS 296

Query: 233 EFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVR---SLDVYLPRDEAFLRIKQVTLLAK 292
           E  RP LGGK+ PYPRRCRTGR    SD  +E R    L +Y+PRDE F   KQ T  A 
Sbjct: 297 ELSRPKLGGKEVPYPRRCRTGRQSTVSDKDAESRVEKPLPMYVPRDEQFEESKQDTFAAG 356

Query: 293 TFYSMINGLKPALEA-IVPD--------TKLH----VPHLSTTDDVPNKGTWNLNTLLPK 352
              ++++ L P+L+A IV +         +L+    +  L   DD+  K        LPK
Sbjct: 357 RLKAVLHHLIPSLKASIVAEDFADFGEIDRLYKEGLLLKLGFQDDIFKKFP------LPK 416

Query: 353 -LIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKL 412
            ++D + +    +L   + +   KDK  W RD+EFARQ +AG+NP +I  VK +P  S L
Sbjct: 417 VVVDTLQESTKGLLKYDTPKILSKDKNAWLRDDEFARQAIAGINPVNIERVKTFPPVSNL 476

Query: 413 EPAIYGSPESAITDEIVEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTL 472
           +P IYG   SA+TD+ +   + G  +V +A+E+N+LY+LDYHD+FLP++ ++  L G+  
Sbjct: 477 DPKIYGPQHSALTDDHIIGHLDG-FSVQQALEENRLYMLDYHDIFLPFLDRINALDGRKA 536

Query: 473 YGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVLAHD 532
           Y +RT+FFL    TL+P+AIELS PP   + + + V TP  DAT  W+W++AKAHV ++D
Sbjct: 537 YATRTIFFLTRLGTLKPVAIELSLPPHGPKHRSKRVLTPPVDATSNWMWQLAKAHVSSND 596

Query: 533 TGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINEG 592
            G HQLV+HWLRTH  +EP+I+AAHRQLSAMHPI++LL PH RYT+E NA+ARQ+LI+  
Sbjct: 597 AGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINALARQSLISAD 656

Query: 593 GIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDYP 652
           G+IE  F+ G Y ME S+ AY   W+F++E LPADLI RG+A+ D   PHGLKL IEDYP
Sbjct: 657 GVIEGGFTAGAYGMEMSAAAYKSSWRFDMEGLPADLIRRGMAIPDATQPHGLKLLIEDYP 716

Query: 653 YANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQVL 712
           YANDGL+LW AI+ W   YV  YYP+P+++K+D ELQ+W++E   VGH D +D  WW  L
Sbjct: 717 YANDGLLLWSAIQTWVRTYVERYYPNPNLIKTDSELQSWYSESINVGHADLRDADWWPEL 776

Query: 713 NTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFLE 772
           +T +DL+ ++TT++W+ S  HA +NFGQY +  Y PNRP + R  +P E   + +  F+ 
Sbjct: 777 STVDDLVSILTTLIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPE-YASFIS 836

Query: 773 KPENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYLGKDIEPA-WADEPHIKRAFEKFSG 832
            PE  +  + P+  Q +   +V++ LS+HS DE+Y+G+  +P+ W  +  I  AF  F+ 
Sbjct: 837 HPEKYYFSSMPSLAQTSKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVEAFYGFAA 896

Query: 833 KLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           ++ ++E+ I++RNA+P  +NR GAGV PY+LL P SEPGVT +GVP S+SI
Sbjct: 897 EIGRIEKEIEKRNADPDRRNRCGAGVLPYELLVPSSEPGVTCRGVPNSVSI 919

BLAST of MS010224 vs. TAIR 10
Match: AT1G72520.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 735.7 bits (1898), Expect = 4.2e-212
Identity = 370/832 (44.47%), Postives = 530/832 (63.70%), Query Frame = 0

Query: 53  KTLVLEILSAEIDPSASPLFSSPYFLYYNIYFLKLVTATGLEKGTIKGYGQKVNKEDDEV 112
           + +VLE++S ++DP  +    S                   +   +K + +K N + + V
Sbjct: 123 RNVVLELMSTQVDPKTNEPKKS-------------------KAAVLKDWSKKSNSKAERV 182

Query: 113 IMYESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSKSQSDD 172
             Y ++F V + FG+ GA+ V N+H +E FLE I I+G   GP+ F CNSW+ S+     
Sbjct: 183 -HYTAEFTVDSAFGSPGAITVTNKHQKEFFLESITIEGFACGPVHFPCNSWVQSQKDHPS 242

Query: 173 RRIFFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGD 232
           +RI FT + YLPS+TP GL+  R +EL NL+GNG G+R+   RIYDYDVYND+GN D   
Sbjct: 243 KRILFTNQPYLPSETPSGLRTLREKELENLRGNGKGERKLSDRIYDYDVYNDIGNPDISR 302

Query: 233 EFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVR---SLDVYLPRDEAFLRIKQVTLLAK 292
           E  RP LGG+++PYPRRCRTGR+   +D +SE R    L +Y+PRDE F   KQ T  A 
Sbjct: 303 ELARPTLGGREFPYPRRCRTGRSSTDTDMMSERRVEKPLPMYVPRDEQFEESKQNTFAAC 362

Query: 293 TFYSMINGLKPALEAIVPDTKLH-------------VPHLSTTDDVPNKGTWNLNTLLPK 352
              ++++ L P+L+A +                   +  L   DD+  K        LPK
Sbjct: 363 RLKAVLHNLIPSLKASILAEDFANFGEIDSLYKEGLLLKLGFQDDMFKKFP------LPK 422

Query: 353 LIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLE 412
           ++  +      +L   + +   KDK+ W RD+EFARQ +AG+NP +I  V  +P  S L+
Sbjct: 423 IVTTLQKSSEGLLRYDTPKIVSKDKYAWLRDDEFARQAIAGINPVNIERVTSYPPVSNLD 482

Query: 413 PAIYG-SPESAITDEIVEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTL 472
           P IYG    SA+T++ +  Q+ G +TV +A+E N+L+++DYHD++LP++ ++  L G+  
Sbjct: 483 PEIYGPGLHSALTEDHIIGQLDG-LTVQQALETNRLFMVDYHDIYLPFLDRINALDGRKA 542

Query: 473 YGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQ-WRDVFTPCWDATGLWLWRIAKAHVLAH 532
           Y +RT+ FL    TL+P+AIELS P      Q  + V TP  DAT  W+W++AKAHV ++
Sbjct: 543 YATRTILFLTRLGTLKPIAIELSLPSQSSSNQKSKRVVTPPVDATSNWMWQLAKAHVGSN 602

Query: 533 DTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLINE 592
           D G HQLV+HWLRTH  +EP+I+AAHRQLSAMHPI++LL PH RYT+E NA+ARQTLI+ 
Sbjct: 603 DAGVHQLVNHWLRTHACLEPFILAAHRQLSAMHPIFKLLDPHMRYTLEINAVARQTLISA 662

Query: 593 GGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIEDY 652
            G+IE+ F+ G+Y +E SS AY  +W+F++E LPADLI RG+AV DP  PHGLKL +EDY
Sbjct: 663 DGVIESCFTAGQYGLEISSAAYKNKWRFDMEGLPADLIRRGMAVPDPTQPHGLKLLVEDY 722

Query: 653 PYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQV 712
           PYANDGL+LW AI+ W   YV  YY + +++++D ELQAW++E   VGH D +D  WW  
Sbjct: 723 PYANDGLLLWSAIQTWVRTYVERYYANSNLIQTDTELQAWYSESINVGHADHRDAEWWPK 782

Query: 713 LNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYFL 772
           L+T EDL+ V+TTI+W+ S  HA +NFGQY +  Y PNRP + R  +P E   + +  F+
Sbjct: 783 LSTVEDLVSVITTIIWLASAQHAALNFGQYPYGGYVPNRPPLMRRLIPDESDPE-FTSFI 842

Query: 773 EKPENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYLGKDIEPA-WADEPHIKRAFEKFS 832
           E P+  F  + P+  Q T   +V++ LS+HS DE+Y+G+  +P+ W  +  I  AF  FS
Sbjct: 843 EDPQKYFFSSMPSLLQTTKFMAVVDTLSTHSPDEEYIGERQQPSIWTGDAEIVDAFYGFS 902

Query: 833 GKLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
            ++ ++E+ ID+RN +P  +NR GAGV PY+L+ P SEPGVT +GVP S+SI
Sbjct: 903 AEIGRIEKEIDKRNRDPSRRNRCGAGVLPYELMAPSSEPGVTCRGVPNSVSI 926

BLAST of MS010224 vs. TAIR 10
Match: AT1G67560.1 (PLAT/LH2 domain-containing lipoxygenase family protein )

HSP 1 Score: 710.3 bits (1832), Expect = 1.9e-204
Identity = 355/775 (45.81%), Postives = 507/775 (65.42%), Query Frame = 0

Query: 108 EDDEVIMYESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLPTGPLRFSCNSWLNSK 167
           +D   +++ + F VP  FG  GA+LV N  + E+ L +I+I+   T  + F  N+W++SK
Sbjct: 147 KDPRYLVFTADFTVPINFGKPGAILVTNLLSTEICLSEIIIED-STDTILFPANTWIHSK 206

Query: 168 SQSDDRRIFFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGN 227
           + +   RI F ++  LPS+TP G+K  R ++L +++G+G G+R+ H RIYDYDVYNDLG+
Sbjct: 207 NDNPQARIIFRSQPCLPSETPDGIKELREKDLVSVRGDGKGERKPHERIYDYDVYNDLGD 266

Query: 228 SDKGDEFKRPVLGGKQYPYPRRCRTGRARYRSDPLSEVRSL---DVYLPRDEAFLRIKQV 287
             K  E  RPVLG  + PYPRRCRTGR     DP  E R     + Y+PRDE F  IK+ 
Sbjct: 267 PRK-TERVRPVLGVPETPYPRRCRTGRPLVSKDPPCESRGKEKEEFYVPRDEVFEEIKRD 326

Query: 288 TLLAKTFYSMINGLKPALEAIVPDTKLHVPHLSTTDDVPNKG---------TWNLNTLLP 347
           T  A  F ++ + L P++ A + +  +     S  D++                L   + 
Sbjct: 327 TFRAGRFKALFHNLVPSIAAALSNLDIPFTCFSDIDNLYKSNIVLGHTEPKDTGLGGFIG 386

Query: 348 KLIDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKL 407
             ++ +++    +L   +      D+F W RD EF RQ LAG+NP +I L+KE P++S L
Sbjct: 387 GFMNGILNVTETLLKYDTPAVIKWDRFAWLRDNEFGRQALAGVNPVNIELLKELPIRSNL 446

Query: 408 EPAIYGSPESAITDEIVEQQIKGI-MTVNEAVEQNKLYILDYHDLFLPYVAKVRKLK--G 467
           +PA+YG  ES +T+EI+ ++++    T+ +A+E+ +L+++DYHD+ LP+V K+  +K   
Sbjct: 447 DPALYGPQESVLTEEIIAREVEHYGTTIEKALEEKRLFLVDYHDILLPFVEKINSIKEDP 506

Query: 468 KTLYGSRTLFFLNPDNTLRPLAIELSAPPIDGQPQWRDVFTPCWDATGLWLWRIAKAHVL 527
           +  Y SRT+FF + +  LRPLAIELS PP   + + + V+T   DAT  W+W++AKAHV 
Sbjct: 507 RKTYASRTIFFYSKNGALRPLAIELSLPP-TAESENKFVYTHGHDATTHWIWKLAKAHVC 566

Query: 528 AHDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLI 587
           ++D G HQLV+HWLRTH S+EPYIIA +RQLS MHP+Y+LLHPH RYT+E NA AR++LI
Sbjct: 567 SNDAGVHQLVNHWLRTHASMEPYIIATNRQLSTMHPVYKLLHPHMRYTLEINARARKSLI 626

Query: 588 NEGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIE 647
           N GGIIE+ F+PGKY+ME SS AY   W+F++E LPADL+ RG+A ED +A  G++L I+
Sbjct: 627 NGGGIIESCFTPGKYAMELSSAAYKSMWRFDMEGLPADLVRRGMAEEDSSAECGVRLVID 686

Query: 648 DYPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWW 707
           DYPYA DGL++W AIK+    YV H+Y D   + SD ELQAWW EI+  GH DKKDEPWW
Sbjct: 687 DYPYAADGLLIWKAIKDLVESYVKHFYSDSKSITSDLELQAWWDEIKNKGHYDKKDEPWW 746

Query: 708 QVLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKY 767
             LNT +DL  ++T ++W+ SG HA +NFGQY F  Y PNRP++ R  +P E     ++ 
Sbjct: 747 PKLNTTQDLSQILTNMIWIASGQHAAINFGQYPFGGYVPNRPTLLRKLIPQETDPD-YEM 806

Query: 768 FLEKPENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYL--GKDIEPAWADEPHIKRAFE 827
           F+  P+  FL + PTQ QAT V +V   LS+HS DE+YL   ++++  W  +  + + F 
Sbjct: 807 FMRNPQYSFLGSLPTQLQATKVMAVQETLSTHSPDEEYLIELREVQRHWFQDEQVVKYFN 866

Query: 828 KFSGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
           KFS +L K+E+ I+ERN + KLKNR GAG+ PY+LL P S  GVTG+G+P SISI
Sbjct: 867 KFSEELVKIEKTINERNKDKKLKNRTGAGMPPYELLLPTSPHGVTGRGIPNSISI 917

BLAST of MS010224 vs. TAIR 10
Match: AT1G55020.1 (lipoxygenase 1 )

HSP 1 Score: 658.7 bits (1698), Expect = 6.6e-189
Identity = 357/773 (46.18%), Postives = 474/773 (61.32%), Query Frame = 0

Query: 115 YESKFEVPAEFGAIGAVLVENQHNEELFLEDIVIQGLP-TGPLRFSCNSWLNSKSQSDDR 174
           ++  F+   +FG  GA L+ N H  E  L+ + ++ +P  G + + CNSW+         
Sbjct: 97  FKVTFDYETDFGYPGAFLIRNSHFSEFLLKSLTLEDVPGHGRVHYICNSWIYPAKHYTTD 156

Query: 175 RIFFTTKSYLPSKTPGGLKRSRAEELRNLQGNGYGKREAHHRIYDYDVYNDLGNSDKGDE 234
           R+FF+ K+YLP +TP  L + R EEL +L+G G G+ +   R+YDY  YNDLG   K   
Sbjct: 157 RVFFSNKTYLPHETPATLLKYREEELVSLRGTGEGELKEWDRVYDYAYYNDLGVPPKN-- 216

Query: 235 FKRPVLGGKQ-YPYPRRCRTGRARYRSDPLSEVR-----SLDVYLPRDEAFLRIKQVTLL 294
             RPVLGG Q YPYPRR RTGR   + DP +E R     SLD+Y+PRDE F  +K    L
Sbjct: 217 -PRPVLGGTQEYPYPRRGRTGRKPTKEDPQTESRLPITSSLDIYVPRDERFGHLKMSDFL 276

Query: 295 AKTFYSMINGLKPALEAIVPDTKLHVPHLSTT-------DDVPNKGTWN---LNTLLPKL 354
           A    ++   ++PALEA+  DT                  D+PN+   +    N  L  L
Sbjct: 277 AYALKAIAQFIQPALEAVFDDTPKEFDSFEDVLKIYEEGIDLPNQALIDSIVKNIPLEML 336

Query: 355 IDFVVDKGVDILPLASSENFGKDKFFWFRDEEFARQPLAGLNPYSIRLVKEWPLKSKLEP 414
            +     G   L     +   +DK  W  DEEFAR+ LAGLNP  I+L+KE+P KSKL+ 
Sbjct: 337 KEIFRTDGQKFLKFPVPQVIKEDKTAWRTDEEFAREMLAGLNPVVIQLLKEFPPKSKLDS 396

Query: 415 AIYGSPESAITDEIVEQQIKGIMTVNEAVEQNKLYILDYHDLFLPYVAKVRKLKGKTLYG 474
             YG+  S IT   +E  + G +TV EA+E+ +L+ILD+HD  +PY+ +V     KT Y 
Sbjct: 397 ESYGNQNSTITKSHIEHNLDG-LTVEEALEKERLFILDHHDTLMPYLGRVNTTTTKT-YA 456

Query: 475 SRTLFFLNPDNTLRPLAIELSAPPIDGQP--QWRDVFTPCWDATGLW--LWRIAKAHVLA 534
           SRTL FL  D TL+PL IELS P  +G       +V+TP     G++  LW++AKA V  
Sbjct: 457 SRTLLFLKDDGTLKPLVIELSLPHPNGDKFGAVSEVYTP---GEGVYDSLWQLAKAFVGV 516

Query: 535 HDTGHHQLVSHWLRTHCSVEPYIIAAHRQLSAMHPIYRLLHPHFRYTMETNAIARQTLIN 594
           +D+G+HQL+SHW++TH S+EP++IA +RQLS +HP+++LL PHFR TM  NA+ARQ LIN
Sbjct: 517 NDSGNHQLISHWMQTHASIEPFVIATNRQLSVLHPVFKLLEPHFRDTMNINALARQILIN 576

Query: 595 EGGIIETTFSPGKYSMEFSSVAYAKQWQFNLEALPADLIHRGLAVEDPNAPHGLKLTIED 654
            GGI E T  P KY+ME SS  Y   W F  +ALPA+L  RG+AVEDP APHGL+L I+D
Sbjct: 577 GGGIFEITVFPSKYAMEMSSFIYKNHWTFPDQALPAELKKRGMAVEDPEAPHGLRLRIKD 636

Query: 655 YPYANDGLILWDAIKEWATEYVNHYYPDPSVVKSDDELQAWWTEIRTVGHGDKKDEPWWQ 714
           YPYA DGL +W AI+ W  +Y+  +Y     +++D ELQAWW E+R  GHGDKK EPWW 
Sbjct: 637 YPYAVDGLEVWYAIESWVRDYIFLFYKIEEDIQTDTELQAWWKEVREEGHGDKKSEPWWP 696

Query: 715 VLNTPEDLIDVVTTIMWVTSGHHAVVNFGQYSFAKYFPNRPSIARVNMPTEEPMKLWKYF 774
            + T E+L++  T I+WV S  HA VNFGQY  A Y PNRP+I+R  MP E   +  +  
Sbjct: 697 KMQTREELVESCTIIIWVASALHAAVNFGQYPVAGYLPNRPTISRQYMPKENTPEFEE-- 756

Query: 775 LEK-PENVFLDTFPTQTQATIVSSVLNILSSHSSDEKYLGKDIEPAWADEPHIKRAFEKF 834
           LEK P+ VFL T   Q Q  +  S++ ILS+HSSDE YLG+     WA E     AFEKF
Sbjct: 757 LEKNPDKVFLKTITAQLQTLLGISLIEILSTHSSDEVYLGQRDSKEWAAEKEALEAFEKF 816

Query: 835 SGKLKKLEEIIDERNANPKLKNRHGAGVEPYQLLKPYSEPGVTGKGVPYSISI 866
             K+K++E+ IDERN +  LKNR G    PY LL P SE GVTG+G+P S+SI
Sbjct: 817 GEKVKEIEKNIDERNDDETLKNRTGLVKMPYTLLFPSSEGGVTGRGIPNSVSI 859