MS010214 (gene) Bitter gourd (TR) v1

Overview


Name	MS010214
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Glucose-methanol-choline (GMC) oxidoreductase family protein
Location	scaffold391: 755585 .. 755749 (-)
RNA-Seq Expression	MS010214
Synteny	MS010214

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TACCATGGCGAATGCCTTGTGAACAAAGTAGTTGATTGTAATTTCAGGATTATTGGAATTGATGCTCTTCGAGTTGTTGATGGTTCGATATTCACCATGTCACGAGAGACAAATCCTTCGTATCCTATTTATTTCTTTTTTTTTTTCCGTTTCCAATTTGTTCCT

mRNA sequence

TACCATGGCGAATGCCTTGTGAACAAAGTAGTTGATTGTAATTTCAGGATTATTGGAATTGATGCTCTTCGAGTTGTTGATGGTTCGATATTCACCATGTCACGAGAGACAAATCCTTCGTATCCTATTTATTTCTTTTTTTTTTTCCGTTTCCAATTTGTTCCT

Coding sequence (CDS)

TACCATGGCGAATGCCTTGTGAACAAAGTAGTTGATTGTAATTTCAGGATTATTGGAATTGATGCTCTTCGAGTTGTTGATGGTTCGATATTCACCATGTCACGAGAGACAAATCCTTCGTATCCTATTTATTTCTTTTTTTTTTTCCGTTTCCAATTTGTTCCT

Protein sequence

YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNPSYPIYFFFFFRFQFVP

Homology

[Close]

BLAST of MS010214 vs. NCBI nr
Match: XP_027122611.1 ((R)-mandelonitrile lyase-like [Coffea arabica] >XP_027126715.1 (R)-mandelonitrile lyase-like [Coffea arabica] >CDO97767.1 unnamed protein product [Coffea canephora])

HSP 1 Score: 66.2 bits (160), Expect = 9.8e-08
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR++GI ALRVVDGSIFT+S  TNP
Sbjct: 489 YHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNP 527

[Close]

BLAST of MS010214 vs. NCBI nr
Match: XP_027168687.1 ((R)-mandelonitrile lyase-like [Coffea eugenioides])

HSP 1 Score: 66.2 bits (160), Expect = 9.8e-08
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR++GI ALRVVDGSIFT+S  TNP
Sbjct: 455 YHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNP 493

[Close]

BLAST of MS010214 vs. NCBI nr
Match: KAA8541557.1 (hypothetical protein F0562_022709 [Nyssa sinensis])

HSP 1 Score: 65.9 bits (159), Expect = 1.3e-07
Identity = 29/39 (74.36%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR+IGID+LR+VDGS FT+S  TNP
Sbjct: 494 YHGGCLVGKVVDRNFRVIGIDSLRIVDGSTFTVSPGTNP 532

[Close]

BLAST of MS010214 vs. NCBI nr
Match: XP_030478881.1 ((R)-mandelonitrile lyase 1 isoform X1 [Cannabis sativa])

HSP 1 Score: 63.2 bits (152), Expect = 8.3e-07
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD +F++IGID+LRVVDGS FT+S  TNP
Sbjct: 498 YHGGCLVGKVVDGDFKVIGIDSLRVVDGSTFTISPGTNP 536

[Close]

BLAST of MS010214 vs. NCBI nr
Match: XP_030478882.1 ((R)-mandelonitrile lyase 1 isoform X2 [Cannabis sativa])

HSP 1 Score: 63.2 bits (152), Expect = 8.3e-07
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD +F++IGID+LRVVDGS FT+S  TNP
Sbjct: 497 YHGGCLVGKVVDGDFKVIGIDSLRVVDGSTFTISPGTNP 535

[Close]

BLAST of MS010214 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 3.0e-07
Identity = 24/39 (61.54%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KV+D +FR+ GIDALRVVDGS F  +  ++P
Sbjct: 487 YHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHP 525

[Close]

BLAST of MS010214 vs. ExPASy Swiss-Prot
Match: Q9S746 (Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 8.6e-07
Identity = 23/39 (58.97%), Postives = 28/39 (71.79%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVV  N +++G+D LRV+DGS F  S  TNP
Sbjct: 530 YHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNP 568

[Close]

BLAST of MS010214 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.1e-06
Identity = 23/39 (58.97%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KV+D +FR+ GI+ALRVVDGS F  +  ++P
Sbjct: 487 YHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHP 525

[Close]

BLAST of MS010214 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 1.5e-06
Identity = 23/39 (58.97%), Postives = 29/39 (74.36%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KV+D  FR+ GI+ALRVVDGS F  +  ++P
Sbjct: 488 YHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHP 526

[Close]

BLAST of MS010214 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.3e-06
Identity = 22/39 (56.41%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV +V+D +FR+ GI+ALRVVDGS F  +  ++P
Sbjct: 489 YHGGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHP 527

[Close]

BLAST of MS010214 vs. ExPASy TrEMBL
Match: A0A6P6X6P1 ((R)-mandelonitrile lyase-like OS=Coffea arabica OX=13443 GN=LOC113739561 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.8e-08
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR++GI ALRVVDGSIFT+S  TNP
Sbjct: 489 YHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNP 527

[Close]

BLAST of MS010214 vs. ExPASy TrEMBL
Match: A0A068TP29 (GMC_OxRdtase_N domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T00021661001 PE=3 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 4.8e-08
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR++GI ALRVVDGSIFT+S  TNP
Sbjct: 489 YHGGCLVGKVVDRNFRVLGIQALRVVDGSIFTVSPGTNP 527

[Close]

BLAST of MS010214 vs. ExPASy TrEMBL
Match: A0A5J5BIC5 (GMC_OxRdtase_N domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_022709 PE=3 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 6.2e-08
Identity = 29/39 (74.36%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD NFR+IGID+LR+VDGS FT+S  TNP
Sbjct: 494 YHGGCLVGKVVDRNFRVIGIDSLRIVDGSTFTVSPGTNP 532

[Close]

BLAST of MS010214 vs. ExPASy TrEMBL
Match: A0A803QAP7 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.0e-07
Identity = 28/39 (71.79%), Postives = 33/39 (84.62%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVVD +F++IGID+LRVVDGS FT+S  TNP
Sbjct: 460 YHGGCLVGKVVDGDFKVIGIDSLRVVDGSTFTISPGTNP 498

[Close]

BLAST of MS010214 vs. ExPASy TrEMBL
Match: A0A7J6DV07 (GMC_oxred_C domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_002345 PE=4 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 5.3e-07
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 1  YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMS 35
          YHG CLV KVVD NFR+IGIDALRVVDGSIFT+S
Sbjct: 15 YHGGCLVGKVVDRNFRVIGIDALRVVDGSIFTVS 48

[Close]

BLAST of MS010214 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 57.8 bits (138), Expect = 3.3e-09
Identity = 24/43 (55.81%), Postives = 31/43 (72.09%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNPSYPI 44
           YHG C V KVVD N++++G+DALR++DGS F  S  TNP   I
Sbjct: 510 YHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATI 552

[Close]

BLAST of MS010214 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 4.2e-09
Identity = 24/39 (61.54%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG C V +VVD N+R++GID+LRV+DGS F  S  TNP
Sbjct: 519 YHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNP 557

[Close]

BLAST of MS010214 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 57.4 bits (137), Expect = 4.2e-09
Identity = 24/39 (61.54%), Postives = 30/39 (76.92%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG C V +VVD N+R++GID+LRV+DGS F  S  TNP
Sbjct: 486 YHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNP 524

[Close]

BLAST of MS010214 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 57.0 bits (136), Expect = 5.5e-09
Identity = 24/39 (61.54%), Postives = 29/39 (74.36%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG C V KVVD N++++GID LRV+DGS F  S  TNP
Sbjct: 535 YHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFLKSPGTNP 573

[Close]

BLAST of MS010214 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 53.5 bits (127), Expect = 6.1e-08
Identity = 23/39 (58.97%), Postives = 28/39 (71.79%), Query Frame = 0


Query: 1   YHGECLVNKVVDCNFRIIGIDALRVVDGSIFTMSRETNP 40
           YHG CLV KVV  N +++G+D LRV+DGS F  S  TNP
Sbjct: 530 YHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNP 568

The following BLAST results are available for this feature:

BLAST of MS010214 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_027122611.1	9.8e-08	76.92	(R)-mandelonitrile lyase-like [Coffea arabica] >XP_027126715.1 (R)-mandelonitril...	[more]
XP_027168687.1	9.8e-08	76.92	(R)-mandelonitrile lyase-like [Coffea eugenioides]	[more]
KAA8541557.1	1.3e-07	74.36	hypothetical protein F0562_022709 [Nyssa sinensis]	[more]
XP_030478881.1	8.3e-07	71.79	(R)-mandelonitrile lyase 1 isoform X1 [Cannabis sativa]	[more]
XP_030478882.1	8.3e-07	71.79	(R)-mandelonitrile lyase 1 isoform X2 [Cannabis sativa]	[more]

BLAST of MS010214 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52706	3.0e-07	61.54	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q9S746	8.6e-07	58.97	Protein HOTHEAD OS=Arabidopsis thaliana OX=3702 GN=HTH PE=1 SV=1	[more]
Q945K2	1.1e-06	58.97	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
P52707	1.5e-06	58.97	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
O50048	3.3e-06	56.41	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]

BLAST of MS010214 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6P6X6P1	4.8e-08	76.92	(R)-mandelonitrile lyase-like OS=Coffea arabica OX=13443 GN=LOC113739561 PE=3 SV...	[more]
A0A068TP29	4.8e-08	76.92	GMC_OxRdtase_N domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T...	[more]
A0A5J5BIC5	6.2e-08	74.36	GMC_OxRdtase_N domain-containing protein OS=Nyssa sinensis OX=561372 GN=F0562_02...	[more]
A0A803QAP7	4.0e-07	71.79	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A7J6DV07	5.3e-07	85.29	GMC_oxred_C domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_002345...	[more]

BLAST of MS010214 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56060.1	3.3e-09	55.81	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	4.2e-09	61.54	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	4.2e-09	61.54	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	5.5e-09	61.54	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	6.1e-08	58.97	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..43 e-value: 1.4E-11 score: 46.0
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 9..44 e-value: 2.1E-7 score: 31.6

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010214.1	MS010214.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors