MS010211 (gene) Bitter gourd (TR) v1

Overview


Name	MS010211
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Ribonuclease H-like superfamily protein
Location	scaffold391: 718846 .. 719232 (+)
RNA-Seq Expression	MS010211
Synteny	MS010211

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACTAAACACTGATGCGGCTTGGCATGCGAACCAAGGGGTGGGAGGTCTCGGATGGATAATCAGGAACGAGAAGGGAGAAATTAAGAAAGCAGGAGGACAAACTATCACCGATGCGCGGGACATTACTTACTTGGAGTTAATGGCGATTTGGCTAGGGATTGAAGCGGTTGTGTCTTCTTCTTCGGTCTCGCTAATAATTGAATCGGAGTCATTGGAAGCGATCCATCTTATTCAAGGAATCCAACAGAATGTGACTGAGATCTTGTGGCTGGTGCAGGATATCAAGAATTTTGGTGAGAGAGCACGATTGAAGAGATTCCAGCACGTCCAAAGGGACGGAAATTCTGTTGCCCAAGAAATTGCAAATCGAGCGGCTGATGGTTTG

mRNA sequence

AAACTAAACACTGATGCGGCTTGGCATGCGAACCAAGGGGTGGGAGGTCTCGGATGGATAATCAGGAACGAGAAGGGAGAAATTAAGAAAGCAGGAGGACAAACTATCACCGATGCGCGGGACATTACTTACTTGGAGTTAATGGCGATTTGGCTAGGGATTGAAGCGGTTGTGTCTTCTTCTTCGGTCTCGCTAATAATTGAATCGGAGTCATTGGAAGCGATCCATCTTATTCAAGGAATCCAACAGAATGTGACTGAGATCTTGTGGCTGGTGCAGGATATCAAGAATTTTGGTGAGAGAGCACGATTGAAGAGATTCCAGCACGTCCAAAGGGACGGAAATTCTGTTGCCCAAGAAATTGCAAATCGAGCGGCTGATGGTTTG

Coding sequence (CDS)

AAACTAAACACTGATGCGGCTTGGCATGCGAACCAAGGGGTGGGAGGTCTCGGATGGATAATCAGGAACGAGAAGGGAGAAATTAAGAAAGCAGGAGGACAAACTATCACCGATGCGCGGGACATTACTTACTTGGAGTTAATGGCGATTTGGCTAGGGATTGAAGCGGTTGTGTCTTCTTCTTCGGTCTCGCTAATAATTGAATCGGAGTCATTGGAAGCGATCCATCTTATTCAAGGAATCCAACAGAATGTGACTGAGATCTTGTGGCTGGTGCAGGATATCAAGAATTTTGGTGAGAGAGCACGATTGAAGAGATTCCAGCACGTCCAAAGGGACGGAAATTCTGTTGCCCAAGAAATTGCAAATCGAGCGGCTGATGGTTTG

Protein sequence

KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQEIANRAADGL

Homology

[Close]

BLAST of MS010211 vs. NCBI nr
Match: XP_022148737.1 (uncharacterized protein LOC111017329 [Momordica charantia])

HSP 1 Score: 250.0 bits (637), Expect = 1.1e-62
Identity = 128/129 (99.22%), Postives = 128/129 (99.22%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAWHANQGVGGLGWIIRNEK EIKKAGGQTITDARDITYLELMAIWLGIEAVVSS
Sbjct: 14  KLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 73

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 120
           SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE
Sbjct: 74  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 133

Query: 121 IANRAADGL 130
           IANRAADGL
Sbjct: 134 IANRAADGL 142

[Close]

BLAST of MS010211 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 110.5 bits (275), Expect = 1.1e-20
Identity = 56/127 (44.09%), Postives = 83/127 (65.35%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNT+AAW A+   GG+GWI+R+EKGE+ KA  + I   R+ITYLE+MAI  G+ A+   
Sbjct: 88  KLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQE 147

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 120
               + +ES+SLEAIHL+    Q+ TEI+WL+++I    +   +   +H+ R+ N VA  
Sbjct: 148 HCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHG 207

Query: 121 IANRAAD 128
           +A RA +
Sbjct: 208 LARRAME 214

[Close]

BLAST of MS010211 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 109.4 bits (272), Expect = 2.4e-20
Identity = 58/135 (42.96%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEA---- 60
           KLNTDAAW A+   GG+GWI+R+EKGE+ KA  + I   R+ITYLE+MAI  G+ A    
Sbjct: 88  KLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQE 147

Query: 61  ----VVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQR 120
               +       + +ES+SLEAIHL+    Q+ TEI+WL+++I    E  ++   +H+ R
Sbjct: 148 HCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISR 207

Query: 121 DGNSVAQEIANRAAD 128
           + N VA ++A RA +
Sbjct: 208 EANKVAHDLARRAME 222

[Close]

BLAST of MS010211 vs. NCBI nr
Match: XP_022154990.1 (uncharacterized protein LOC111022134 isoform X1 [Momordica charantia])

HSP 1 Score: 83.2 bits (204), Expect = 1.8e-12
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAW A+    G+GWI+R+EKGE+ K G + I   R+ITYLE+MAI  G+ A+   
Sbjct: 264 KLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQE 323

Query: 61  SSVSLIIESESLEAIHLI 79
               + +ES+SLEAIHL+
Sbjct: 324 HCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS010211 vs. NCBI nr
Match: XP_022154991.1 (uncharacterized protein LOC111022134 isoform X2 [Momordica charantia])

HSP 1 Score: 83.2 bits (204), Expect = 1.8e-12
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAW A+    G+GWI+R+EKGE+ K G + I   R+ITYLE+MAI  G+ A+   
Sbjct: 229 KLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQE 288

Query: 61  SSVSLIIESESLEAIHLI 79
               + +ES+SLEAIHL+
Sbjct: 289 HCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS010211 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 5.4e-63
Identity = 128/129 (99.22%), Postives = 128/129 (99.22%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAWHANQGVGGLGWIIRNEK EIKKAGGQTITDARDITYLELMAIWLGIEAVVSS
Sbjct: 14  KLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 73

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 120
           SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE
Sbjct: 74  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 133

Query: 121 IANRAADGL 130
           IANRAADGL
Sbjct: 134 IANRAADGL 142

[Close]

BLAST of MS010211 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 5.2e-21
Identity = 56/127 (44.09%), Postives = 83/127 (65.35%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNT+AAW A+   GG+GWI+R+EKGE+ KA  + I   R+ITYLE+MAI  G+ A+   
Sbjct: 88  KLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAICEGLRAIRQE 147

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 120
               + +ES+SLEAIHL+    Q+ TEI+WL+++I    +   +   +H+ R+ N VA  
Sbjct: 148 HCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRHISREANKVAHG 207

Query: 121 IANRAAD 128
           +A RA +
Sbjct: 208 LARRAME 214

[Close]

BLAST of MS010211 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 1.1e-20
Identity = 58/135 (42.96%), Postives = 85/135 (62.96%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEA---- 60
           KLNTDAAW A+   GG+GWI+R+EKGE+ KA  + I   R+ITYLE+MAI  G+ A    
Sbjct: 88  KLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAICEGLRAIRQE 147

Query: 61  ----VVSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQR 120
               +       + +ES+SLEAIHL+    Q+ TEI+WL+++I    E  ++   +H+ R
Sbjct: 148 HCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMKIVSMRHISR 207

Query: 121 DGNSVAQEIANRAAD 128
           + N VA ++A RA +
Sbjct: 208 EANKVAHDLARRAME 222

[Close]

BLAST of MS010211 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 8.8e-13
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAW A+    G+GWI+R+EKGE+ K G + I   R+ITYLE+MAI  G+ A+   
Sbjct: 264 KLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQE 323

Query: 61  SSVSLIIESESLEAIHLI 79
               + +ES+SLEAIHL+
Sbjct: 324 HCRPIHLESDSLEAIHLL 341

[Close]

BLAST of MS010211 vs. ExPASy TrEMBL
Match: A0A6J1DQC9 (uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 8.8e-13
Identity = 41/78 (52.56%), Postives = 55/78 (70.51%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           KLNTDAAW A+    G+GWI+R+EKGE+ K G + I   R+ITYLE+MAI  G+ A+   
Sbjct: 229 KLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVMAICEGLRAIRQE 288

Query: 61  SSVSLIIESESLEAIHLI 79
               + +ES+SLEAIHL+
Sbjct: 289 HCRPIHLESDSLEAIHLL 306

[Close]

BLAST of MS010211 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 59.7 bits (143), Expect = 2.0e-09
Identity = 33/125 (26.40%), Postives = 65/125 (52.00%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           K NTDA W+ +    G+GW++RNEKGE+K  G + +   + +   EL A+   + ++   
Sbjct: 429 KCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRF 488

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSVAQE 120
               +I ES+S   I ++    +    +   +QD++    +    +F  + R+GN++A+ 
Sbjct: 489 QYNYVIFESDSQVLIEILNN-DEIWPSLKPTIQDLQRLLSQFTEVKFVFIPREGNTLAER 548

Query: 121 IANRA 126
           +A  +
Sbjct: 549 VARES 552

[Close]

BLAST of MS010211 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 5.5e-07
Identity = 33/130 (25.38%), Postives = 59/130 (45.38%), Query Frame = 0


Query: 1   KLNTDAAWHANQGVGGLGWIIRNEKGEIKKAGGQTITDARDITYLELMAIWLGIEAVVSS 60
           K NTDA W       G+GWI+RNE G +   G + +   +++   EL A+   +  +   
Sbjct: 146 KCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEAELEALRWAVLTMSRF 205

Query: 61  SSVSLIIESESLEAIHLIQGIQQNVTEILW-----LVQDIKNFGERARLKRFQHVQRDGN 120
           +   +I ES++   ++L+       ++  W      ++DI+         +F+   R GN
Sbjct: 206 NYKRIIFESDAQALVNLLN------SDDFWPTLQPALEDIQQLLHHFEEVKFEFTPRGGN 265

Query: 121 SVAQEIANRA 126
            VA  IA  +
Sbjct: 266 KVADRIARES 269

The following BLAST results are available for this feature:

BLAST of MS010211 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148737.1	1.1e-62	99.22	uncharacterized protein LOC111017329 [Momordica charantia]	[more]
XP_022143535.1	1.1e-20	44.09	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022156777.1	2.4e-20	42.96	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022154990.1	1.8e-12	52.56	uncharacterized protein LOC111022134 isoform X1 [Momordica charantia]	[more]
XP_022154991.1	1.8e-12	52.56	uncharacterized protein LOC111022134 isoform X2 [Momordica charantia]	[more]

BLAST of MS010211 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of MS010211 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D5W1	5.4e-63	99.22	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1CP26	5.2e-21	44.09	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DSV1	1.1e-20	42.96	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DL64	8.8e-13	52.56	uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1DQC9	8.8e-13	52.56	uncharacterized protein LOC111022134 isoform X2 OS=Momordica charantia OX=3673 G...	[more]

BLAST of MS010211 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29090.1	2.0e-09	26.40	Ribonuclease H-like superfamily protein	[more]
AT2G34320.1	5.5e-07	25.38	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..128 e-value: 2.3E-15 score: 58.8
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 3..125 e-value: 3.7E-20 score: 72.0
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 1..126
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 2..123 e-value: 4.99308E-18 score: 71.9616
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..128

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010211.1	MS010211.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity