Homology
BLAST of MS010200 vs. NCBI nr
Match:
XP_022148831.1 (uncharacterized protein LOC111017390 [Momordica charantia])
HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0
Query: 1 MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK 60
MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK
Sbjct: 1 MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK 60
Query: 61 GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD 120
GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD
Sbjct: 61 GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD 120
Query: 121 NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN 180
NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN 180
Query: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS 240
TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS 240
Query: 241 QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG 300
QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG
Sbjct: 241 QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG 300
Query: 301 VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR 360
VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR
Sbjct: 301 VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR 360
Query: 361 RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG 420
RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG
Sbjct: 361 RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG 420
Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES 480
LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES 480
Query: 481 DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF
Sbjct: 481 DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 538
BLAST of MS010200 vs. NCBI nr
Match:
XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])
HSP 1 Score: 943.7 bits (2438), Expect = 6.8e-271
Identity = 469/541 (86.69%), Postives = 493/541 (91.13%), Query Frame = 0
Query: 1 MVRVGVKP-SWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSS 60
M+R+ VKP WCS I +++ PL PSI RPVL PRLFLTR SASS L K+ E SS
Sbjct: 1 MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLE--SS 60
Query: 61 SAKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
S KG SD L PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61 SPKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
Query: 121 DFDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDV 180
DF+NFWSECPWD+DLKY KAVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDV
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180
Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLS 240
LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMD+PSVLELSKD VKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
Query: 241 HLSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
HLSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
Query: 301 EEGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
+EGVILEAESGDFLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
Query: 361 KRRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNED 420
KRRR+FRVGSLKWLT PPDQF+GLQCKVRHGPSFYSCNLTME +EDG EDIAVVHL+ED
Sbjct: 361 KRRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSED 420
Query: 421 DQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVK 480
DQGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E A MEDKSKLGKP+KIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVK 480
Query: 481 PESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQI 539
PES LKE E K+DTK IHGE NQ TT ++QIGE SSDEAIT APMKWLG+L KKLLQ+
Sbjct: 481 PESVLKESEAKDDTK--IHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQV 537
BLAST of MS010200 vs. NCBI nr
Match:
XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 941.8 bits (2433), Expect = 2.6e-270
Identity = 464/540 (85.93%), Postives = 495/540 (91.67%), Query Frame = 0
Query: 1 MVRVGVKPSWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSS 60
M+R+ VKP WC+ I +++ PLRPSI RPVL +PRLFLTR SASS + K+SE S S
Sbjct: 1 MLRLRVKPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASE--SYS 60
Query: 61 AKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
AKG SDG L+PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61 AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
Query: 121 FDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVL 180
F+NFWSECPWD+DLKY +AVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSH 240
CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMD+PSVLELSKD VKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
Query: 241 LSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEE 300
LSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE+
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
Query: 301 EGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
EGVILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
Query: 361 RRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDD 420
RRRLFRVGSLKWL+ PDQFT LQCKVRHGPSFYSCNLTMER+ED ED+AVVHL+EDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420
Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKP 480
QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALEIA MEDKSKLGKP+KIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
Query: 481 ESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
E+ KE E K+DTK IHG L+NQ+TT VEQ GE +SDEA+T APMKWLG+L KKLLQ+F
Sbjct: 481 ENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
BLAST of MS010200 vs. NCBI nr
Match:
XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])
HSP 1 Score: 940.3 bits (2429), Expect = 7.5e-270
Identity = 463/540 (85.74%), Postives = 495/540 (91.67%), Query Frame = 0
Query: 1 MVRVGVKPSWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSS 60
M+R+ V P WC+ I +++ PLRPSI RPVL +PRLFLTR SASS + K+SE SSS
Sbjct: 1 MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASE--SSS 60
Query: 61 AKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
AKG SDG L+PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61 AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
Query: 121 FDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVL 180
F+NFWSECPWD+DLKY KAVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSH 240
CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM++PSVLELSKD VKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240
Query: 241 LSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEE 300
LSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE+
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
Query: 301 EGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
EGVILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
Query: 361 RRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDD 420
RRRLFRVGSLKWL+ PDQFT LQCKVRHGPSFYSCNLTMER+ED ED+AVVHL+EDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420
Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKP 480
QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALEIA MEDKSKLGKP+KIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
Query: 481 ESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
E+ KE E K+DTK IHG L+NQ+TT VEQ GE +SDEA+T APMKWLG+L KKLLQ+F
Sbjct: 481 ENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
BLAST of MS010200 vs. NCBI nr
Match:
KAG6570391.1 (hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 937.2 bits (2421), Expect = 6.4e-269
Identity = 462/540 (85.56%), Postives = 494/540 (91.48%), Query Frame = 0
Query: 1 MVRVGVKPSWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSS 60
M+R+ V P WC+ I +++ PLRPSI RPVL +PRLFLTR SASS + K+SE SSS
Sbjct: 1 MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASE--SSS 60
Query: 61 AKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
AKG SDG L+PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61 AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
Query: 121 FDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVL 180
F+NFWSECPWD+DLKY KAVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSH 240
CNTRIKFGAFMDAISNMEF+YVASGHYANVIHPSADQM++PSVLELSKD VKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFNYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240
Query: 241 LSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEE 300
LSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE+
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
Query: 301 EGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
EGVILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
Query: 361 RRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDD 420
RRRLFRVGSLKWL+ PDQFT LQCKVRHGPSFYSCNLTMER+E ED+AVVHL+EDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEGSDEDVAVVHLSEDD 420
Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKP 480
QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALEIA MEDKSKLGKP+KIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
Query: 481 ESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
E+ KE E K+DTK IHG L+NQ+TT VEQ GE +SDEA+T APMKWLG+L KKLLQ+F
Sbjct: 481 ENASKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
BLAST of MS010200 vs. ExPASy Swiss-Prot
Match:
Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)
HSP 1 Score: 370.5 bits (950), Expect = 3.1e-101
Identity = 171/365 (46.85%), Postives = 251/365 (68.77%), Query Frame = 0
Query: 80 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAV 139
+K+AVLLSGGVDSSVAL R+++ S +YLKIW +++ ++ CPW EDL YV+AV
Sbjct: 1 MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60
Query: 140 CDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
C + +VP E+++ +EY+ VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61 CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120
Query: 200 YVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDE 259
V +GHYA + D+ L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180
Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFW 319
VR++A NLPNK+RKDSQGICFLGKIK++EF+ H+GE++G I+E E+G +GTH G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240
Query: 320 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQ 379
F+T+GQR+G++L GPW+V+EKD++ N++++S N + +R F V + W+ +P D
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300
Query: 380 FTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGS 439
F + K+RHG YSC L + +++ + LN+ D G++ GQFA FY+ C+G
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLIA-----KNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352
Query: 440 GVILE 444
I +
Sbjct: 361 AKIFK 352
BLAST of MS010200 vs. ExPASy Swiss-Prot
Match:
O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)
HSP 1 Score: 369.4 bits (947), Expect = 6.9e-101
Identity = 169/365 (46.30%), Postives = 250/365 (68.49%), Query Frame = 0
Query: 80 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAV 139
+K+AVLLSGGVDSSVAL R+++ + +YLKIW +++ ++ CPW EDL YV+A+
Sbjct: 1 MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60
Query: 140 CDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
C++ +VP E+++ +EY+ VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61 CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120
Query: 200 YVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDE 259
V +GHYA + + +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180
Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFW 319
VR++A NLPNKDRKDSQGICFLGKIK++EF+ H+GE++G I+E E+G +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240
Query: 320 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQ 379
F+T+GQR+G++L GPW+V+EKD++ N++++S N + +R F V + W+ P
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIN-DTPTN 300
Query: 380 FTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGS 439
F + K+RHG YSC L + + ++ + LN+ DQG++ GQFA FY+ C+G
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352
Query: 440 GVILE 444
I +
Sbjct: 361 AKIFK 352
BLAST of MS010200 vs. ExPASy Swiss-Prot
Match:
B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)
HSP 1 Score: 369.4 bits (947), Expect = 6.9e-101
Identity = 169/365 (46.30%), Postives = 250/365 (68.49%), Query Frame = 0
Query: 80 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAV 139
+K+AVLLSGGVDSSVAL R+++ + +YLKIW +++ ++ CPW EDL YV+A+
Sbjct: 1 MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60
Query: 140 CDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
C++ +VP E+++ +EY+ VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61 CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120
Query: 200 YVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDE 259
V +GHYA + + +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180
Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFW 319
VR++A NLPNKDRKDSQGICFLGKIK++EF+ H+GE++G I+E E+G +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240
Query: 320 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQ 379
F+T+GQR+G++L GPW+V+EKD++ N++++S N + +R F V + W+ P
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWIN-DTPTN 300
Query: 380 FTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGS 439
F + K+RHG YSC L + + ++ + LN+ DQG++ GQFA FY+ C+G
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352
Query: 440 GVILE 444
I +
Sbjct: 361 AKIFK 352
BLAST of MS010200 vs. ExPASy Swiss-Prot
Match:
Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)
HSP 1 Score: 363.6 bits (932), Expect = 3.8e-99
Identity = 166/365 (45.48%), Postives = 249/365 (68.22%), Query Frame = 0
Query: 80 LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAV 139
+K+AVLLSGGVDSS+AL ++++ + +YLKIW +++ ++ CPW EDL YV+AV
Sbjct: 1 MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60
Query: 140 CDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
C++ ++P E+++ +EY+ VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61 CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120
Query: 200 YVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDE 259
V +GHYA + ++ L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180
Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFW 319
VR++A NLPNKDRKDSQGICFLGKIK++EF+ H+GE++G I+E E+G +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240
Query: 320 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQ 379
F+T+GQR+G++L GPW+V+EKD++ N++++S N + +R F V + W+ P D
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300
Query: 380 FTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGS 439
F + K+RHG YSC L + + ++ + LN+ D G++ GQFA FY+ C+G
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352
Query: 440 GVILE 444
I +
Sbjct: 361 AKIFK 352
BLAST of MS010200 vs. ExPASy Swiss-Prot
Match:
B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)
HSP 1 Score: 360.9 bits (925), Expect = 2.5e-98
Identity = 171/366 (46.72%), Postives = 247/366 (67.49%), Query Frame = 0
Query: 80 LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAV 139
+K+AVLLSGGVDSSVAL + G+ + +YLKIW +++ ++ ECPW+ED+ YV+AV
Sbjct: 1 MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60
Query: 140 CDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 199
C + +VP E++ L +EY+K VV+Y IEE + G TP+PD+ CN RIKFGAF D I N +D
Sbjct: 61 CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120
Query: 200 YVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDE 259
+ +GHYA + + + +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180
Query: 260 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFW 319
+R++A K +LPNK+RKDSQGICFLGKIK+ EF+ H+GE +G I+E E+G LGTH G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240
Query: 320 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQ 379
F+TIGQR+G++L GPW+V EKDI+NN++++S + + + F V W ++ P
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNW--INKPVN 300
Query: 380 FTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGS 439
L K+RHG C + M ++ DI V L E D G++ GQF FY+ C+G
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352
Query: 440 GVILES 445
IL++
Sbjct: 361 AQILQT 352
BLAST of MS010200 vs. ExPASy TrEMBL
Match:
A0A6J1D6J8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Momordica charantia OX=3673 GN=LOC111017390 PE=3 SV=1)
HSP 1 Score: 1107.4 bits (2863), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0
Query: 1 MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK 60
MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK
Sbjct: 1 MVRVGVKPSWCSIFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSSAK 60
Query: 61 GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD 120
GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD
Sbjct: 61 GFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFD 120
Query: 121 NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN 180
NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN
Sbjct: 121 NFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVLCN 180
Query: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS 240
TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS
Sbjct: 181 TRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSHLS 240
Query: 241 QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG 300
QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG
Sbjct: 241 QYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEEEG 300
Query: 301 VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR 360
VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR
Sbjct: 301 VILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRR 360
Query: 361 RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG 420
RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG
Sbjct: 361 RLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDDQG 420
Query: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES 480
LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES
Sbjct: 421 LAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKPES 480
Query: 481 DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF
Sbjct: 481 DLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 538
BLAST of MS010200 vs. ExPASy TrEMBL
Match:
A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)
HSP 1 Score: 940.3 bits (2429), Expect = 3.6e-270
Identity = 463/540 (85.74%), Postives = 495/540 (91.67%), Query Frame = 0
Query: 1 MVRVGVKPSWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSS 60
M+R+ V P WC+ I +++ PLRPSI RPVL +PRLFLTR SASS + K+SE SSS
Sbjct: 1 MLRLRVNPVWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASE--SSS 60
Query: 61 AKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
AKG SDG L+PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61 AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
Query: 121 FDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVL 180
F+NFWSECPWD+DLKY KAVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSH 240
CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM++PSVLELSKD VKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240
Query: 241 LSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEE 300
LSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE+
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
Query: 301 EGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
EGVILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
Query: 361 RRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDD 420
RRRLFRVGSLKWL+ PDQFT LQCKVRHGPSFYSCNLTMER+ED ED+AVVHL+EDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420
Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKP 480
QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC KALEIA MEDKSKLGKP+KIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
Query: 481 ESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
E+ KE E K+DTK IHG L+NQ+TT VEQ GE +SDEA+T APMKWLG+L KKLLQ+F
Sbjct: 481 ENATKECEPKDDTK--IHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
BLAST of MS010200 vs. ExPASy TrEMBL
Match:
A0A6J1JC61 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=LOC111483706 PE=3 SV=1)
HSP 1 Score: 934.1 bits (2413), Expect = 2.6e-268
Identity = 461/540 (85.37%), Postives = 493/540 (91.30%), Query Frame = 0
Query: 1 MVRVGVKPSWCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSSS 60
M+R+ VKP WC+ I +++ LRPSI RPVL +PRLF+TR SASS + K+SE SSS
Sbjct: 1 MLRLRVKPVWCTLPISHFTSLTLRPSILRPVLRRHRPRLFITRFSASSAVHKASE--SSS 60
Query: 61 AKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
AKG SDG L+PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61 AKGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
Query: 121 FDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDVL 180
F+NFWSECPWD+DLKY KAVCD+VDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDVL
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDRVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLSH 240
CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMD+PSVLELSKD VKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
Query: 241 LSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEE 300
LSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE+
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
Query: 301 EGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
EGVILEAESGDFLG HRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360
Query: 361 RRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNEDD 420
RRR FRVGSLKWL+ PDQFTGLQCKVRHGPSFYSC LTMERDED ED+AVV L+EDD
Sbjct: 361 RRRSFRVGSLKWLSGLVPDQFTGLQCKVRHGPSFYSCILTMERDEDSDEDVAVVLLSEDD 420
Query: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVKP 480
QGLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KALEIA MEDKSKLGKP+KIKVKP
Sbjct: 421 QGLAAGQFAAFYQGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480
Query: 481 ESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQIF 539
E+ KE E K+DTK IHG L+NQ+TT VEQ GE +SDEA+T APMKWLG+L KKLLQ+F
Sbjct: 481 ENATKECETKDDTK--IHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536
BLAST of MS010200 vs. ExPASy TrEMBL
Match:
A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)
HSP 1 Score: 916.0 bits (2366), Expect = 7.4e-263
Identity = 455/541 (84.10%), Postives = 484/541 (89.46%), Query Frame = 0
Query: 1 MVRVGVKPS-WCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSS 60
M+R+ V P+ WCS I +++ PL PSI RP+L PRLFLTR SASS + +S SSSS
Sbjct: 2 MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61
Query: 61 SAKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
K SD PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 62 --KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 121
Query: 121 DFDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDV 180
DF+NFWSECPWD+DLKY KAVCDQVDVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDV
Sbjct: 122 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 181
Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLS 240
LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMD+PSVLELSKD VKDQTYFLS
Sbjct: 182 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 241
Query: 241 HLSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
HLSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 242 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 301
Query: 301 EEGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
+EGVILEAESGDFLG HRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 302 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 361
Query: 361 KRRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNED 420
KRRR+FRVGSLKWL+ PPDQFT LQCKVRHGPSFYSCNLTME EDGHED+AVVHL+ED
Sbjct: 362 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 421
Query: 421 DQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVK 480
DQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC+KA+E A MEDKSKLGKP+KIKVK
Sbjct: 422 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 481
Query: 481 PESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQI 539
PES +KE + K+DT EIH +LRN+ T VEQI E S DEA+ MKWLG+L KKLLQI
Sbjct: 482 PESGMKESKAKDDT--EIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQI 538
BLAST of MS010200 vs. ExPASy TrEMBL
Match:
A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)
HSP 1 Score: 906.7 bits (2342), Expect = 4.5e-260
Identity = 446/541 (82.44%), Postives = 482/541 (89.09%), Query Frame = 0
Query: 1 MVRVGVKPS-WCS--IFNYSFPPLRPSIFRPVLCFSKPRLFLTRSSASSLLLKSSESSSS 60
M+R+ V P+ WCS I +++ PL PSI RP+L PRLF TR SASS ++ + S SS
Sbjct: 2 MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASS-AVQHNTSQSS 61
Query: 61 SAKGFSDGLLDPFLSCSMPNYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
K SD PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 62 LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 121
Query: 121 DFDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTEEYWKNVVSYIIEEYRCGRTPNPDV 180
DF+NFWSECPWD+DLKY KAVCDQ+DVPLEVVHLTEEYW+NVVSYIIEEYRCGRTPNPDV
Sbjct: 122 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 181
Query: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDRPSVLELSKDTVKDQTYFLS 240
LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMD+PSVLELSKD VKDQTYFLS
Sbjct: 182 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 241
Query: 241 HLSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
HLSQ QLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 242 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 301
Query: 301 EEGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
+EGVILEAESGDFLG HRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 302 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 361
Query: 361 KRRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFYSCNLTMERDEDGHEDIAVVHLNED 420
KRRR+FRVGSLKWL+ PPDQFTGLQCKVRHGP FYSCNLTME +EDGHED+AVVHL+ED
Sbjct: 362 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 421
Query: 421 DQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCEKALEIAGMEDKSKLGKPVKIKVK 480
DQGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC+KA+E A MEDKSKLGKP+KIKVK
Sbjct: 422 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 481
Query: 481 PESDLKEHEHKEDTKIEIHGELRNQRTTAVEQIGETSSDEAITPAPMKWLGNLGKKLLQI 539
P+S + E + K+DTKI+ E RN+ T VEQ+ + S DEA+ PMKWLG+L KKLLQI
Sbjct: 482 PDSGMNESKAKDDTKIQ--DESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQI 539
BLAST of MS010200 vs. TAIR 10
Match:
AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )
HSP 1 Score: 708.0 bits (1826), Expect = 5.9e-204
Identity = 343/445 (77.08%), Postives = 382/445 (85.84%), Query Frame = 0
Query: 42 SSASSLLLKSSESSS------SSAKGFSDG--LLDPFLSCSMPNYPLKVAVLLSGGVDSS 101
SS+SS L S +SS+ SS + +S ++D +LSCSMP PL+VAVLLSGGVDSS
Sbjct: 36 SSSSSSQLPSPQSSNPITPLISSNRNYSRHAFIVDQYLSCSMPEKPLRVAVLLSGGVDSS 95
Query: 102 VALRLLHAAGHSCTAFYLKIWFQEDFDNFWSECPWDEDLKYVKAVCDQVDVPLEVVHLTE 161
VALRLLHAAGHSCTAFYLKIWFQE F+NFW++CPW++DLKY K VC+QVDVPLEVVHLT+
Sbjct: 96 VALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWEDDLKYAKHVCEQVDVPLEVVHLTD 155
Query: 162 EYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSA 221
EYW+ VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+ME+DYV SGHYA V+HP A
Sbjct: 156 EYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISDMEYDYVGSGHYAKVVHPPA 215
Query: 222 DQMDRPSVLELSKDTVKDQTYFLSHLSQYQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDR 281
DQ D SVLELS+D VKDQTYFLSHLSQ QLKRLLFPLGC+ KDEVRKLA +F+LPNKDR
Sbjct: 216 DQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLLFPLGCVKKDEVRKLATQFDLPNKDR 275
Query: 282 KDSQGICFLGKIKFSEFVARHIGEEEGVILEAESGDFLGTHRGFWFYTIGQRQGLRLPGG 341
KDSQGICFLGKIKFS+FV RHIGE EG+ILEAESGDFLG HRGFWFYTIGQRQGLRLPGG
Sbjct: 276 KDSQGICFLGKIKFSDFVCRHIGEMEGIILEAESGDFLGNHRGFWFYTIGQRQGLRLPGG 335
Query: 342 PWYVVEKDIKNNVVFVSRNYFSFDKRRRLFRVGSLKWLTVSPPDQFTGLQCKVRHGPSFY 401
PWYVVEKD KNNVVFVSRNY+S DKRRR+FRVGSL+W + P + L+CKVRHGP FY
Sbjct: 336 PWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWNSGKPSGKVRELRCKVRHGPGFY 395
Query: 402 SCNLTMERDEDGHEDIAVVHLNEDDQGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVC 461
SC+ ME G D+AVVHL+EDDQGLAAGQFAAFYEG CIGSGVILESWDDQ FPVC
Sbjct: 396 SCSFEME----GDGDVAVVHLDEDDQGLAAGQFAAFYEGTTCIGSGVILESWDDQCFPVC 455
Query: 462 EKALEIAGMEDKSKLGKPVKIKVKP 479
KAL++A +EDK+KLGKPVKI P
Sbjct: 456 AKALQLAALEDKTKLGKPVKIMTMP 476
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022148831.1 | 0.0e+00 | 100.00 | uncharacterized protein LOC111017390 [Momordica charantia] | [more] |
| XP_038901654.1 | 6.8e-271 | 86.69 | tRNA-specific 2-thiouridylase MnmA [Benincasa hispida] | [more] |
| XP_023513286.1 | 2.6e-270 | 85.93 | uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo] | [more] |
| XP_022944568.1 | 7.5e-270 | 85.74 | uncharacterized protein LOC111448983 [Cucurbita moschata] | [more] |
| KAG6570391.1 | 6.4e-269 | 85.56 | hypothetical protein SDJN03_29306, partial [Cucurbita argyrosperma subsp. sorori... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q0SMH1 | 3.1e-101 | 46.85 | tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN... | [more] |
| O51625 | 6.9e-101 | 46.30 | tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... | [more] |
| B7J2N7 | 6.9e-101 | 46.30 | tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... | [more] |
| Q660I8 | 3.8e-99 | 45.48 | tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496... | [more] |
| B2S125 | 2.5e-98 | 46.72 | tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1D6J8 | 0.0e+00 | 100.00 | tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Momordica charantia OX=3673 G... | [more] |
| A0A6J1FY63 | 3.6e-270 | 85.74 | tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN... | [more] |
| A0A6J1JC61 | 2.6e-268 | 85.37 | tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita maxima OX=3661 GN=L... | [more] |
| A0A0A0KX16 | 7.4e-263 | 84.10 | tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs... | [more] |
| A0A5A7TDN6 | 4.5e-260 | 82.44 | tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT1G51310.1 | 5.9e-204 | 77.08 | transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases | [more] |
