MS010187 (gene) Bitter gourd (TR) v1

BLAST of MS010187 vs. NCBI nr
Match: XP_022148720.1 (uncharacterized protein LOC111017315 [Momordica charantia])

HSP 1 Score: 833.9 bits (2153), Expect = 6.3e-238
Identity = 444/450 (98.67%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREITNK 120
           EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREI NK
Sbjct: 61  EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREIINK 120

Query: 121 KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLERLRRGSRDKQM 180
           KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEIN LGQKLERLRRGSRDKQM
Sbjct: 121 KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINYLGQKLERLRRGSRDKQM 180

Query: 181 EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN 240
           EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN
Sbjct: 181 EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN 240

Query: 241 LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM 300
           LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM
Sbjct: 241 LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM 300

Query: 301 DQVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM 360
           +QVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM
Sbjct: 301 EQVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM 360

Query: 361 AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDE-- 420
           AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDE  
Sbjct: 361 AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDEQQ 420

Query: 421 -QQQKNRSQIKKLEKSQRLPFRDIANLVHN 448
            QQQKNRSQIKKLEKSQRLPFRDIANLVHN
Sbjct: 421 QQQQKNRSQIKKLEKSQRLPFRDIANLVHN 450

BLAST of MS010187 vs. NCBI nr
Match: XP_022944587.1 (calponin homology domain-containing protein DDB_G0272472 isoform X2 [Cucurbita moschata])

HSP 1 Score: 640.6 bits (1651), Expect = 1.0e-179
Identity = 367/480 (76.46%), Postives = 399/480 (83.12%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV+L+          EER++ G G+A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVALMDRGEGRSERVEERENVGVGSAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLEEEINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEEEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKGACGDHESFISSGKF 240

Query: 241 NVEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDSSM- 300
           NVEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDSSM 
Sbjct: 241 NVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDSSMP 300

Query: 301 ---SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360
                 D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQVSRE
Sbjct: 301 THLPNPDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360

Query: 361 FWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMK--GHGKASLPDEMEKHV 420
           FWKD+ALESESQI+SLQSSVEEWK KA+A+E+K+KE ERSK K  GHG+AS PDEMEKHV
Sbjct: 361 FWKDQALESESQIRSLQSSVEEWKHKAIAHETKAKEVERSKKKGQGHGQASPPDEMEKHV 420

Query: 421 LICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRDIAN 444
           LICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRDI N
Sbjct: 421 LICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKTFEKSQRLPFRDITN 478

BLAST of MS010187 vs. NCBI nr
Match: KAG6570403.1 (hypothetical protein SDJN03_29318, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639.8 bits (1649), Expect = 1.7e-179
Identity = 368/480 (76.67%), Postives = 398/480 (82.92%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV L+          EER++ G G+A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVVLMDRGEGRSERVEERENVGVGSAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLEEEINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEEEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATEMKGACGDHESFISSGKF 240

Query: 241 NVEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDSSM- 300
           NVEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDSSM 
Sbjct: 241 NVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDSSMP 300

Query: 301 ---SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360
                 D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQVSRE
Sbjct: 301 THLPNPDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360

Query: 361 FWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMK--GHGKASLPDEMEKHV 420
           FWKD+ALESESQI+SLQSSVEEWK KA+A+E+K+KE ERSK K  GHG+AS PDEMEKHV
Sbjct: 361 FWKDQALESESQIRSLQSSVEEWKHKAIAHETKAKEVERSKKKGQGHGQASPPDEMEKHV 420

Query: 421 LICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRDIAN 444
           LICR+KEKNPN LH RDVRSR REIAIDE      QQQK  S IK  EKSQRLPFRDI N
Sbjct: 421 LICRVKEKNPNLLHARDVRSRRREIAIDEQQLQQQQQQKIHSHIKTFEKSQRLPFRDITN 478

BLAST of MS010187 vs. NCBI nr
Match: XP_022944586.1 (calponin homology domain-containing protein DDB_G0272472 isoform X1 [Cucurbita moschata])

HSP 1 Score: 635.2 bits (1637), Expect = 4.3e-178
Identity = 367/483 (75.98%), Postives = 399/483 (82.61%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV+L+          EER++ G G+A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVALMDRGEGRSERVEERENVGVGSAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLEEEINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEEEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKGACGDHESFISSGKF 240

Query: 241 N---VEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDS 300
           N   VEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDS
Sbjct: 241 NNVQVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDS 300

Query: 301 SM----SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQV 360
           SM       D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQV
Sbjct: 301 SMPTHLPNPDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQV 360

Query: 361 SREFWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMK--GHGKASLPDEME 420
           SREFWKD+ALESESQI+SLQSSVEEWK KA+A+E+K+KE ERSK K  GHG+AS PDEME
Sbjct: 361 SREFWKDQALESESQIRSLQSSVEEWKHKAIAHETKAKEVERSKKKGQGHGQASPPDEME 420

Query: 421 KHVLICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRD 444
           KHVLICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRD
Sbjct: 421 KHVLICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKTFEKSQRLPFRD 480

BLAST of MS010187 vs. NCBI nr
Match: XP_022987000.1 (calponin homology domain-containing protein DDB_G0272472 isoform X2 [Cucurbita maxima])

HSP 1 Score: 634.0 bits (1634), Expect = 9.6e-178
Identity = 365/481 (75.88%), Postives = 395/481 (82.12%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV L+          EER+  G  +A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVVLMDRGEGRSERVEEREIVGVASAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLE+EINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEDEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKDACGDHESFISSGKF 240

Query: 241 NVEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDSSMS 300
           NVEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDSSM 
Sbjct: 241 NVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDSSMP 300

Query: 301 RQ----DWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360
                 D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQVSRE
Sbjct: 301 THLPNLDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360

Query: 361 FWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMKG---HGKASLPDEMEKH 420
           FWKD+ALESESQI+SLQSSVEEWK K +A+ESK+KE ERSK KG   HG+AS PDEMEKH
Sbjct: 361 FWKDQALESESQIRSLQSSVEEWKHKVIAHESKTKEVERSKKKGQGRHGQASPPDEMEKH 420

Query: 421 VLICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRDIA 444
           VLICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRDI 
Sbjct: 421 VLICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKAFEKSQRLPFRDIT 479

BLAST of MS010187 vs. ExPASy TrEMBL
Match: A0A6J1D3Q0 (uncharacterized protein LOC111017315 OS=Momordica charantia OX=3673 GN=LOC111017315 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.1e-238
Identity = 444/450 (98.67%), Postives = 445/450 (98.89%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREITNK 120
           EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREI NK
Sbjct: 61  EALFDQEREFSRESFPVSLLEERKSGGGGAAPVEEKWRFQAEMLRAECNLLRMEREIINK 120

Query: 121 KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLERLRRGSRDKQM 180
           KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEIN LGQKLERLRRGSRDKQM
Sbjct: 121 KLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINYLGQKLERLRRGSRDKQM 180

Query: 181 EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN 240
           EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN
Sbjct: 181 EVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENKSCRDHESFVSSGKLNVEVLRRKMEN 240

Query: 241 LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM 300
           LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM
Sbjct: 241 LSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSMSRQDWTSQEQNQCSGHCKAIIRRIM 300

Query: 301 DQVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM 360
           +QVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM
Sbjct: 301 EQVKAEKDQWSQMQEMLNQVREEMEELQVSREFWKDRALESESQIQSLQSSVEEWKQKAM 360

Query: 361 AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDE-- 420
           AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDE  
Sbjct: 361 AYESKSKEAERSKMKGHGKASLPDEMEKHVLICRMKEKNPNLHTRDVRSRPREIAIDEQQ 420

Query: 421 -QQQKNRSQIKKLEKSQRLPFRDIANLVHN 448
            QQQKNRSQIKKLEKSQRLPFRDIANLVHN
Sbjct: 421 QQQQKNRSQIKKLEKSQRLPFRDIANLVHN 450

BLAST of MS010187 vs. ExPASy TrEMBL
Match: A0A6J1FW14 (calponin homology domain-containing protein DDB_G0272472 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448999 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 5.0e-180
Identity = 367/480 (76.46%), Postives = 399/480 (83.12%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV+L+          EER++ G G+A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVALMDRGEGRSERVEERENVGVGSAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLEEEINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEEEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKGACGDHESFISSGKF 240

Query: 241 NVEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDSSM- 300
           NVEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDSSM 
Sbjct: 241 NVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDSSMP 300

Query: 301 ---SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360
                 D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQVSRE
Sbjct: 301 THLPNPDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360

Query: 361 FWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMK--GHGKASLPDEMEKHV 420
           FWKD+ALESESQI+SLQSSVEEWK KA+A+E+K+KE ERSK K  GHG+AS PDEMEKHV
Sbjct: 361 FWKDQALESESQIRSLQSSVEEWKHKAIAHETKAKEVERSKKKGQGHGQASPPDEMEKHV 420

Query: 421 LICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRDIAN 444
           LICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRDI N
Sbjct: 421 LICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKTFEKSQRLPFRDITN 478

BLAST of MS010187 vs. ExPASy TrEMBL
Match: A0A6J1FUT8 (calponin homology domain-containing protein DDB_G0272472 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448999 PE=4 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 2.1e-178
Identity = 367/483 (75.98%), Postives = 399/483 (82.61%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV+L+          EER++ G G+A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVALMDRGEGRSERVEERENVGVGSAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLEEEINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEEEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKGACGDHESFISSGKF 240

Query: 241 N---VEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDS 300
           N   VEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDS
Sbjct: 241 NNVQVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDS 300

Query: 301 SM----SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQV 360
           SM       D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQV
Sbjct: 301 SMPTHLPNPDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQV 360

Query: 361 SREFWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMK--GHGKASLPDEME 420
           SREFWKD+ALESESQI+SLQSSVEEWK KA+A+E+K+KE ERSK K  GHG+AS PDEME
Sbjct: 361 SREFWKDQALESESQIRSLQSSVEEWKHKAIAHETKAKEVERSKKKGQGHGQASPPDEME 420

Query: 421 KHVLICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRD 444
           KHVLICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRD
Sbjct: 421 KHVLICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKTFEKSQRLPFRD 480

BLAST of MS010187 vs. ExPASy TrEMBL
Match: A0A6J1JFL2 (calponin homology domain-containing protein DDB_G0272472 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484579 PE=4 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 4.6e-178
Identity = 365/481 (75.88%), Postives = 395/481 (82.12%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV L+          EER+  G  +A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVVLMDRGEGRSERVEEREIVGVASAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLE+EINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEDEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKDACGDHESFISSGKF 240

Query: 241 NVEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDSSMS 300
           NVEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDSSM 
Sbjct: 241 NVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDSSMP 300

Query: 301 RQ----DWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360
                 D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQVSRE
Sbjct: 301 THLPNLDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQVSRE 360

Query: 361 FWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMKG---HGKASLPDEMEKH 420
           FWKD+ALESESQI+SLQSSVEEWK K +A+ESK+KE ERSK KG   HG+AS PDEMEKH
Sbjct: 361 FWKDQALESESQIRSLQSSVEEWKHKVIAHESKTKEVERSKKKGQGRHGQASPPDEMEKH 420

Query: 421 VLICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFRDIA 444
           VLICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFRDI 
Sbjct: 421 VLICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKAFEKSQRLPFRDIT 479

BLAST of MS010187 vs. ExPASy TrEMBL
Match: A0A6J1JI67 (calponin homology domain-containing protein DDB_G0272472 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484579 PE=4 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 1.9e-176
Identity = 365/484 (75.41%), Postives = 395/484 (81.61%), Query Frame = 0

Query: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKL 60
           MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSK++PA   S  G DGRGKL
Sbjct: 1   MSSLATVTATRRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKSVPAKPSS--GFDGRGKL 60

Query: 61  EALFDQEREFSRESFPVSLL----------EERKSGGGGAAPVEEKWRFQAEMLRAECNL 120
           EALFDQER F ++ FPV L+          EER+  G  +A  EEKWRFQAEMLRAECNL
Sbjct: 61  EALFDQERAFLKDGFPVVLMDRGEGRSERVEEREIVGVASAAAEEKWRFQAEMLRAECNL 120

Query: 121 LRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDLGQKLER 180
           LRMEREITNKKLEKMK+RMERTLKSAVQALVSG+NK+YEGKDM+MVLE+EINDL +KLER
Sbjct: 121 LRMEREITNKKLEKMKVRMERTLKSAVQALVSGKNKVYEGKDMEMVLEDEINDLAKKLER 180

Query: 181 LRRGSRDKQMEVRKCSNFDKRASLLQRKLEKIGEDSDEPIVTENK-SCRDHESFVSSGKL 240
           LRRGSR+KQ+ VRKCSNFDKRASLLQRKLEKI  +S E I TE K +C DHESF+SSGK 
Sbjct: 181 LRRGSRNKQIVVRKCSNFDKRASLLQRKLEKIDGNSGELIATETKDACGDHESFISSGKF 240

Query: 241 N---VEVLRRKMENLSNGTLLEMMREECRSMLSTTAT-------------TKIRAEHPDS 300
           N   VEVLRRKME+LS GTLLE MREECRSMLS TAT             +KIR EHPDS
Sbjct: 241 NNVQVEVLRRKMEDLSKGTLLEKMREECRSMLSITATSSVVSSATSSAASSKIRTEHPDS 300

Query: 301 SMSRQ----DWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQV 360
           SM       D TSQE+NQCSGHCKAIIRRI +QVKAEKDQWSQMQEMLNQVREEMEELQV
Sbjct: 301 SMPTHLPNLDSTSQERNQCSGHCKAIIRRITEQVKAEKDQWSQMQEMLNQVREEMEELQV 360

Query: 361 SREFWKDRALESESQIQSLQSSVEEWKQKAMAYESKSKEAERSKMKG---HGKASLPDEM 420
           SREFWKD+ALESESQI+SLQSSVEEWK K +A+ESK+KE ERSK KG   HG+AS PDEM
Sbjct: 361 SREFWKDQALESESQIRSLQSSVEEWKHKVIAHESKTKEVERSKKKGQGRHGQASPPDEM 420

Query: 421 EKHVLICRMKEKNPN-LHTRDVRSRPREIAIDE------QQQKNRSQIKKLEKSQRLPFR 444
           EKHVLICR+KEKNPN LH RDVRSR REI+IDE      QQQK  S IK  EKSQRLPFR
Sbjct: 421 EKHVLICRVKEKNPNLLHARDVRSRRREISIDEQQLQQQQQQKIHSHIKAFEKSQRLPFR 480

BLAST of MS010187 vs. TAIR 10
Match: AT1G51405.1 (myosin-related )

HSP 1 Score: 265.8 bits (678), Expect = 6.4e-71
Identity = 186/436 (42.66%), Postives = 256/436 (58.72%), Query Frame = 0

Query: 11  RRPKWQYPQPAPPTPRILHFPRRPRTRRRPSKALPAPGKSILGSDGRGKLEALFDQEREF 70
           R+ KWQYPQ   PTPRIL  PRR   RR  SK L     S    D R KLE LF QER F
Sbjct: 11  RKSKWQYPQVGSPTPRILQLPRRHSVRRNASKGLKTTVSSSSQKDRRVKLEVLFHQERTF 70

Query: 71  SRESFPVSLLE---ERK-------------------SGGGGAAPVEE----KWRFQAEML 130
            R +  V + +   E+K                   SG   ++ V+E    KWRFQAEML
Sbjct: 71  DRGASIVMVNDNGGEKKDEEEGRRRGKVADGREIGVSGLSSSSAVDEVEEAKWRFQAEML 130

Query: 131 RAECNLLRMEREITNKKLEKMKLRMERTLKSAVQALVSGRNKIYEGKDMKMVLEEEINDL 190
           R+ECNLLR+E+EI  KK+E+ K RMERTL+SAV  L+SG+ +I EG     VLE+EI+ L
Sbjct: 131 RSECNLLRIEKEIALKKMERRKKRMERTLRSAVLTLLSGKERISEGTKESKVLEDEISYL 190

Query: 191 GQKLERLRRGSRDKQMEVRKCS-NFDKRASLLQRKLEKIGEDSDEPIVTE--------NK 250
            +KL  L +  + K ME R    NFDK+AS+L+R+LE+  E   E +  +        + 
Sbjct: 191 VEKLNEL-KSPKVKDMEARNFKHNFDKQASVLRRELERFDEAVSEEVCVKGIQKMAEASF 250

Query: 251 SCRDHESFVSSGKLNVEVLRRKMENLSNGTLLEMMREECRSMLSTTATTKIRAEHPDSSM 310
           S    ++ + +   N++ L  KME LS G LLE M +E  S L   +++ ++  +     
Sbjct: 251 SVHSDQNALRNNNGNIDTLSSKMEALSKGVLLERMEKEYGSSLVAPSSSSVQDMYCKGIK 310

Query: 311 SRQDWTSQEQNQCSGHCKAIIRRIMDQVKAEKDQWSQMQEMLNQVREEMEELQVSREFWK 370
           +      +E+  CS HCK ++R+I D+V+AE +QWSQMQEMLNQVR+EMEELQ  R+FW+
Sbjct: 311 AH-----EEKKDCSRHCKVVMRKIADEVRAEAEQWSQMQEMLNQVRKEMEELQSCRDFWQ 370

Query: 371 DRALESESQIQSLQSSVEEWKQKAMAYESKSK---------EAERSKMKGHGKA-----S 398
           +RALE++S+IQ+L SSVE W++KA++ E+K K         + E  +++   K       
Sbjct: 371 NRALEADSEIQNLHSSVEGWRRKALSSEAKLKNLQAEVCGLQEEIKRLRKEDKLEPEKNK 430