MS010186 (gene) Bitter gourd (TR) v1

Overview
NameMS010186
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptioncinnamoyl-CoA reductase 1-like
Locationscaffold391: 560209 .. 563007 (-)
RNA-Seq ExpressionMS010186
SyntenyMS010186
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGGCGGAGCGGCGAAGACTGTGTGCGTCACCGGCGCCTCTGGATACATCGCTTCTTGGATTGTCAAGTTCCTTCTCCGGCGGGGTTACACCGTCAAAGCCAGCGTCCGAGATCCGAGTTTGTCTCTCATTTTTCTTCTTCCGCTTCCTCCATCAAATGCATCACAAATTTACTTCACAATATTCTGTTTCCCTACAAATCCTGCTGCCTATTTCATGTTTCCTAGTTTTTGGCGACTTGTTTGATGATCGGTTTGCCTTTCTGTCCCGTAGGCGATCCGATTAAAACTGCACATTTGCTTGCACTCGATGGAGCGGCTGAGAGGCTTCATCTGTTTAAAGCCAATCTCCTAGAGGAAGGTTCATTTGATTCTGCTGTTCAGGGCTGCGAAGGGGTTTTTCATACAGCTTCTCCCTTCTTCCATTCTGTTTCAGATCCACAGGTTTGTCCACTGCAAAGTATGTATGGATTGAGCCTAGAAGATTCAGAGTTTTGTATTGTGTGTCATATATGTCTCTGGAAGTATTATGTTAATGAAACTAGTTTCTGAAGAATTGTGGATGTCGCGTAATAAGAATAATCAACTTATGGAAGAACAATAATTCCCTCAGATTAGCTTTTACTCGTTATAGAAGCAGAAGAAAGAAGACTCTAGGAGTTGTTGTCTTATTCAATTCTATTTATTTCTATCTTTTGTCTTCTTTTTCACTACACAGGTGTTCTTTCTTTCCATTTCTGCAAATATCTTATAGCATGAAACAATTGAAATTTCGTTTTTAATTCATGGAACAAGTAAGAAGTATATGCTGGTCGAGCTGCTCCTTAGTAACCCTGAAATCTTCCATGGTAAAGGGGATACGTTGGCCTTTTAATAAGATTCTTTTTTTCAGGCTGAACTTATTGATCCTGCATTGAAAGGAACACTGAATGTTCTTAAGTCAGTTGCAAAATCATCAACTGTCAAGCGGGTTGTCTTGACATCTTCAATTGCTGCTGTTGCTTATAACGAAAAGCCTCGTACTCCCGATGTTGTAGTTGATGAGACTTGGTTTTCTAGTCCGGAGGTTTGCAAGGAATTGAAGGTATCCATTTCATTCATTTTTCATACTCCCACCATCCTTGGTAAGAAGGCAAAAAACAGAGGAAAAATAAAAGAGTACTTGTGAGCATGACTCCACTTGCTATCGTACCTTGTCATGTGGTACCCCGATAAAAAACCGATGCATACACATAATTCTAACTTCAATTTGCATTCTCAATTTCCTAGAGCTCGCTGAGTTATGGTATCAAAGAGGTTCTTATTGTTAATAATATTGTATGGATTTTTTTTTTAATGTCTTCTTTCTCTGGATTGGATGTCTGTCATTTTGTGCTCCTCTTTCCCTTGATATGCTCCACAAGTTTCTCAACAGAATTGAGAAAGAAAGAGCTCTCATCAGTTTTTTTTCCCTCACCTATATCCCGATTTCAATTAAATAATCAAACATGGAAGTCACACCATATGTCAAAGTTTGTCCTTGTTTCCTATCATATTTCTGTATTACAGTTGCCTCAGTGTTTCAGAAGCTAAATATAAATCCTTTCCTCTTATTTCCTTATGGGTTTCAGCTTTGGTACGTGCTCTCCAAAACTTTAGCTGAGGAGGCAGCCTGGAATTTTGTAAGAGAGAAGGGAATTGACATGGTTACCATAAACCCTGCTATGGTTATCGGTCCATTATTGCAGCCGACTCTGAATACTAGTGCTGAAGCTATTTTGAACTTAATATCTGGTATTTGTTCGGTTATCTGGGGTTATGCTTAACGCGTTTCCTCTTTCCTTTCATCAAACTGTTTTAAGTGCTTAGTAACAATGAACTATACATTGCATCAGAATTCTTCACAAGATCTTTCCGAGTATGATAAATCTATAACCTTGTGGTTTTAATCTAATGCAGGAGCACAAACATTTCCAAATTCCACATTCGGATGGGTTAATGTCAAAGATGTTGCTAATGCACACATTCAAGCCTATGAGATTCCATCAGCTAGTGGAAGATACTGTTTGGTTGAGAGAGTTATTCACTATTCAGGAACTGTGAAGATTTTACGTGATCTCTACCCTTCATTAAAACTTCCAGATAAGTAAGTTTTCCTGATCCACACCTACCCTCCGAAGAACTTTGTTTATTTATTTTTAAATGGCTTTCCCGACAAGAACAGTATCTTAAATTATTTCATCTGCAAACTTGCTTCTTTGTAGCTTCCTTGGCTTTGATTCCTAGCATTTATCCCTAAGTTTCTTTATAAGAGAATCAATCGAACATTTTTGGTAAATCGGACAAAGGTTGGTGGAAGATTCTACAATTTAGCGAATGAGAACAAATAGAAAACTAATTTCATAAAGTGCGTCATGCTCTTTCTTTTGGCTGAACCATATTCTGAGGCTCTGAAATTTCATGACCAGAACTTTATCATATTTTTTATTCTTGCATGAAAGAGCATCAAGGTTTCATAAATCTTTGATCTACAAAGATTTCATATTGCTGTAGTTGGTACAATTAATCTGCACCTCAATCCTCTTGAAGCCGTGATCATTATAGGTGTTATCTGTCATATTCATACTCGGTTTCGACGACAAGTCTAGTTAATCAGTACTACACTTTCAACTGCAGGTGTGCAGATGATAAACCGTTTATACCGACATACCAGGTCTCGATGGAGAAAGCAAAGAGCTTAGGCATTGAATTCATTCCTCTAGAGGAGAGTCTGAAGGAAACCGTTGAGAGCTTGAAGGAAAAGAAATTTGTCAGCTTC

mRNA sequence

ATGAGCGGCGGAGCGGCGAAGACTGTGTGCGTCACCGGCGCCTCTGGATACATCGCTTCTTGGATTGTCAAGTTCCTTCTCCGGCGGGGTTACACCGTCAAAGCCAGCGTCCGAGATCCGAGCGATCCGATTAAAACTGCACATTTGCTTGCACTCGATGGAGCGGCTGAGAGGCTTCATCTGTTTAAAGCCAATCTCCTAGAGGAAGGTTCATTTGATTCTGCTGTTCAGGGCTGCGAAGGGGTTTTTCATACAGCTTCTCCCTTCTTCCATTCTGTTTCAGATCCACAGGCTGAACTTATTGATCCTGCATTGAAAGGAACACTGAATGTTCTTAAGTCAGTTGCAAAATCATCAACTGTCAAGCGGGTTGTCTTGACATCTTCAATTGCTGCTGTTGCTTATAACGAAAAGCCTCGTACTCCCGATGTTGTAGTTGATGAGACTTGGTTTTCTAGTCCGGAGGTTTGCAAGGAATTGAAGCTTTGGTACGTGCTCTCCAAAACTTTAGCTGAGGAGGCAGCCTGGAATTTTGTAAGAGAGAAGGGAATTGACATGGTTACCATAAACCCTGCTATGGTTATCGGTCCATTATTGCAGCCGACTCTGAATACTAGTGCTGAAGCTATTTTGAACTTAATATCTGGAGCACAAACATTTCCAAATTCCACATTCGGATGGGTTAATGTCAAAGATGTTGCTAATGCACACATTCAAGCCTATGAGATTCCATCAGCTAGTGGAAGATACTGTTTGGTTGAGAGAGTTATTCACTATTCAGGAACTGTGAAGATTTTACGTGATCTCTACCCTTCATTAAAACTTCCAGATAAGTGTGCAGATGATAAACCGTTTATACCGACATACCAGGTCTCGATGGAGAAAGCAAAGAGCTTAGGCATTGAATTCATTCCTCTAGAGGAGAGTCTGAAGGAAACCGTTGAGAGCTTGAAGGAAAAGAAATTTGTCAGCTTC

Coding sequence (CDS)

ATGAGCGGCGGAGCGGCGAAGACTGTGTGCGTCACCGGCGCCTCTGGATACATCGCTTCTTGGATTGTCAAGTTCCTTCTCCGGCGGGGTTACACCGTCAAAGCCAGCGTCCGAGATCCGAGCGATCCGATTAAAACTGCACATTTGCTTGCACTCGATGGAGCGGCTGAGAGGCTTCATCTGTTTAAAGCCAATCTCCTAGAGGAAGGTTCATTTGATTCTGCTGTTCAGGGCTGCGAAGGGGTTTTTCATACAGCTTCTCCCTTCTTCCATTCTGTTTCAGATCCACAGGCTGAACTTATTGATCCTGCATTGAAAGGAACACTGAATGTTCTTAAGTCAGTTGCAAAATCATCAACTGTCAAGCGGGTTGTCTTGACATCTTCAATTGCTGCTGTTGCTTATAACGAAAAGCCTCGTACTCCCGATGTTGTAGTTGATGAGACTTGGTTTTCTAGTCCGGAGGTTTGCAAGGAATTGAAGCTTTGGTACGTGCTCTCCAAAACTTTAGCTGAGGAGGCAGCCTGGAATTTTGTAAGAGAGAAGGGAATTGACATGGTTACCATAAACCCTGCTATGGTTATCGGTCCATTATTGCAGCCGACTCTGAATACTAGTGCTGAAGCTATTTTGAACTTAATATCTGGAGCACAAACATTTCCAAATTCCACATTCGGATGGGTTAATGTCAAAGATGTTGCTAATGCACACATTCAAGCCTATGAGATTCCATCAGCTAGTGGAAGATACTGTTTGGTTGAGAGAGTTATTCACTATTCAGGAACTGTGAAGATTTTACGTGATCTCTACCCTTCATTAAAACTTCCAGATAAGTGTGCAGATGATAAACCGTTTATACCGACATACCAGGTCTCGATGGAGAAAGCAAAGAGCTTAGGCATTGAATTCATTCCTCTAGAGGAGAGTCTGAAGGAAACCGTTGAGAGCTTGAAGGAAAAGAAATTTGTCAGCTTC

Protein sequence

MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAYEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEFIPLEESLKETVESLKEKKFVSF
Homology
BLAST of MS010186 vs. NCBI nr
Match: XP_022153022.1 (cinnamoyl-CoA reductase 1-like [Momordica charantia])

HSP 1 Score: 642.9 bits (1657), Expect = 1.5e-180
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH
Sbjct: 57  MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 116

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST
Sbjct: 117 LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 176

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 177 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 236

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA
Sbjct: 237 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 296

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG
Sbjct: 297 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 356

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEKKFVSF
Sbjct: 357 IEFIPLEESLKETVESLKEKKFVSF 381

BLAST of MS010186 vs. NCBI nr
Match: XP_038901182.1 (phenylacetaldehyde reductase-like [Benincasa hispida])

HSP 1 Score: 603.6 bits (1555), Expect = 1.0e-168
Identity = 297/325 (91.38%), Postives = 315/325 (96.92%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTG SGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGGSGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFD+AV+GC+GVFHTASPFFH+ SDPQAELIDPALKGTLNVLKSVAK+S+
Sbjct: 61  LFKANLLEEGSFDTAVEGCQGVFHTASPFFHAASDPQAELIDPALKGTLNVLKSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PRTPD V+DETWFS+PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNGQPRTPDTVIDETWFSNPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YE PSASGRYCLVE+V+HYSG VKIL DLYPSL+LPDKC DDKPFIPTYQVS+EKAKSLG
Sbjct: 241 YETPSASGRYCLVEKVVHYSGIVKILHDLYPSLQLPDKCVDDKPFIPTYQVSVEKAKSLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEK F+SF
Sbjct: 301 IEFIPLEESLKETVESLKEKNFISF 325

BLAST of MS010186 vs. NCBI nr
Match: KAG6605921.1 (Cinnamoyl-CoA reductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035869.1 Cinnamoyl-CoA reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 600.1 bits (1546), Expect = 1.1e-167
Identity = 297/325 (91.38%), Postives = 314/325 (96.62%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGASGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAV+GCEGVFHTASPFFH+VSDPQAELIDPALKGTLNVL SVAK+S+
Sbjct: 61  LFKANLLEEGSFDSAVEGCEGVFHTASPFFHNVSDPQAELIDPALKGTLNVLNSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD VVDETWF+ PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNRQPRNPDTVVDETWFTDPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAIL LISGA+TFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILKLISGAETFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVE+VIH+SG VKIL DLYPSL+LPDKCADDKP +PTYQVS+EKAK LG
Sbjct: 241 YEIPSASGRYCLVEKVIHHSGIVKILHDLYPSLRLPDKCADDKPQVPTYQVSVEKAKGLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEKKF+SF
Sbjct: 301 IEFIPLEESLKETVESLKEKKFISF 325

BLAST of MS010186 vs. NCBI nr
Match: XP_022957943.1 (cinnamoyl-CoA reductase 1-like [Cucurbita moschata])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 296/325 (91.08%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGASGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAV+GCEGVFHTASPFFH+VSDPQAELIDPALKGTLNVL SVAK+S+
Sbjct: 61  LFKANLLEEGSFDSAVEGCEGVFHTASPFFHNVSDPQAELIDPALKGTLNVLNSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD VVDETWF+ PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNRQPRNPDTVVDETWFTDPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAIL LISGA+TFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILKLISGAETFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVE+VIH+SG VKIL DLYPSL+LPDKCADDKP +PTYQVS+EKAK LG
Sbjct: 241 YEIPSASGRYCLVEKVIHHSGIVKILHDLYPSLRLPDKCADDKPQVPTYQVSVEKAKGLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEE LKETVESLKEKKF+SF
Sbjct: 301 IEFIPLEEGLKETVESLKEKKFISF 325

BLAST of MS010186 vs. NCBI nr
Match: XP_022995113.1 (cinnamoyl-CoA reductase 1-like [Cucurbita maxima])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 297/325 (91.38%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGASGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAV+GCEGVFHTASPFFH+VSDPQAELIDPALKGTLNVL SVAK+S+
Sbjct: 61  LFKANLLEEGSFDSAVEGCEGVFHTASPFFHNVSDPQAELIDPALKGTLNVLNSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD VVDETWF+ PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNRQPRNPDTVVDETWFTDPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAIL LISGA+TF NSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILKLISGAETFQNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVE+VIHYSG VKIL DLYPSL+LPDKCADDKP +PTYQVS+EKAK LG
Sbjct: 241 YEIPSASGRYCLVEKVIHYSGIVKILHDLYPSLQLPDKCADDKPQVPTYQVSVEKAKGLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEKKF+SF
Sbjct: 301 IEFIPLEESLKETVESLKEKKFISF 325

BLAST of MS010186 vs. ExPASy Swiss-Prot
Match: A0A0B6VQ48 (Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.4e-151
Identity = 256/319 (80.25%), Postives = 294/319 (92.16%), Query Frame = 0

Query: 7   KTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANL 66
           K VCVTGASGYIASW+VK LL+RGYTVKASVR+P+DP KT HLLALDGA ERL LFKA+L
Sbjct: 4   KVVCVTGASGYIASWLVKLLLQRGYTVKASVRNPNDPTKTEHLLALDGAKERLQLFKADL 63

Query: 67  LEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVL 126
           LEEGSFDSAV+GCEGVFHTASPF+H V+DP+AEL+DPA+KGTLNVL S +KS ++KRVVL
Sbjct: 64  LEEGSFDSAVEGCEGVFHTASPFYHDVTDPKAELLDPAVKGTLNVLNSCSKSPSIKRVVL 123

Query: 127 TSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDM 186
           TSSIAAVAYN KPRTPDVVVDETWF+ P+VCKE KLWYVLSKTLAE+AAW FV+EKGIDM
Sbjct: 124 TSSIAAVAYNGKPRTPDVVVDETWFTDPDVCKESKLWYVLSKTLAEDAAWKFVKEKGIDM 183

Query: 187 VTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAYEIPSA 246
           VTINPAMVIGPLLQPTLNTSA AILN+I GA+T+PN++FGW+NVKDVANAH+QA+EIPSA
Sbjct: 184 VTINPAMVIGPLLQPTLNTSAAAILNIIKGARTYPNASFGWINVKDVANAHVQAFEIPSA 243

Query: 247 SGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEFIPL 306
           SGRYCLVERV H++  ++I+ +LYP L+LP+KC+DDKPF+PTYQVS EKAKSLGIEFIPL
Sbjct: 244 SGRYCLVERVAHFTEVLQIIHELYPDLQLPEKCSDDKPFVPTYQVSKEKAKSLGIEFIPL 303

Query: 307 EESLKETVESLKEKKFVSF 326
           + SLKET+ESLKEK  VSF
Sbjct: 304 DISLKETIESLKEKSIVSF 322

BLAST of MS010186 vs. ExPASy Swiss-Prot
Match: A0A059TC02 (Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 4.8e-81
Identity = 162/322 (50.31%), Postives = 218/322 (67.70%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           M   + + VCVTGA G+IASW+VK LL +GYTV+ +VR+P DP K  HL  L+GA ERL 
Sbjct: 1   MRSVSGQVVCVTGAGGFIASWLVKILLEKGYTVRGTVRNPDDP-KNGHLRELEGAKERLT 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           L KA+LL+  S   A+ GC+GVFHTASP    V+D   ++++PA+ GT NV+ + A+++ 
Sbjct: 61  LCKADLLDYQSLREAINGCDGVFHTASP----VTDDPEQMVEPAVIGTKNVINAAAEAN- 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           V+RVV TSSI AV Y +  R P+ VVDET +S P+ CK  K WY   K +AE+AAW   +
Sbjct: 121 VRRVVFTSSIGAV-YMDPNRDPETVVDETCWSDPDFCKNTKNWYCYGKMVAEQAAWEEAK 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISG-AQTFPNSTFGWVNVKDVANAHIQ 240
           EKG+D+V INP +V GPLLQ T+N S   IL  ++G A+T+ NS   +V+VKDVA AHI 
Sbjct: 181 EKGVDLVVINPVLVQGPLLQTTVNASVLHILKYLTGSAKTYANSVQAYVDVKDVALAHIL 240

Query: 241 AYEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCAD-DKPFIPTYQVSMEKAKS 300
            YE P ASGRY   E V+H    V+IL   +P   +P KC+D  KP +  Y+ S +K K 
Sbjct: 241 LYETPEASGRYLCAESVLHRGDVVEILSKFFPEYPIPTKCSDVTKPRVKPYKFSNQKLKD 300

Query: 301 LGIEFIPLEESLKETVESLKEK 321
           LG+EF P+++ L ETV+SL+EK
Sbjct: 301 LGLEFTPVKQCLYETVKSLQEK 315

BLAST of MS010186 vs. ExPASy Swiss-Prot
Match: Q9S9N9 (Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 4.5e-79
Identity = 159/318 (50.00%), Postives = 214/318 (67.30%), Query Frame = 0

Query: 5   AAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKA 64
           A KTVCVTGA GYIASWIVK LL RGYTVK +VR+P DP K  HL  L+G  ERL L KA
Sbjct: 9   AGKTVCVTGAGGYIASWIVKILLERGYTVKGTVRNPDDP-KNTHLRELEGGKERLILCKA 68

Query: 65  NLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRV 124
           +L +  +  +A+ GC+GVFHTASP    V+D   ++++PA+ G   V+ + A++  VKRV
Sbjct: 69  DLQDYEALKAAIDGCDGVFHTASP----VTDDPEQMVEPAVNGAKFVINAAAEAK-VKRV 128

Query: 125 VLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGI 184
           V+TSSI AV Y +  R P+ VVDE+ +S  + CK  K WY   K +AE+AAW   +EKG+
Sbjct: 129 VITSSIGAV-YMDPNRDPEAVVDESCWSDLDFCKNTKNWYCYGKMVAEQAAWETAKEKGV 188

Query: 185 DMVTINPAMVIGPLLQPTLNTSAEAILNLISG-AQTFPNSTFGWVNVKDVANAHIQAYEI 244
           D+V +NP +V+GP LQPT+N S   +L  ++G A+T+ N T  +V+V+DVA AH+  YE 
Sbjct: 189 DLVVLNPVLVLGPPLQPTINASLYHVLKYLTGSAKTYANLTQAYVDVRDVALAHVLVYEA 248

Query: 245 PSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDK-PFIPTYQVSMEKAKSLGIE 304
           PSASGRY L E   H    V+IL  L+P   LP KC D+K P    Y+ + +K K LG+E
Sbjct: 249 PSASGRYLLAESARHRGEVVEILAKLFPEYPLPTKCKDEKNPRAKPYKFTNQKIKDLGLE 308

Query: 305 FIPLEESLKETVESLKEK 321
           F   ++SL +TV+SL+EK
Sbjct: 309 FTSTKQSLYDTVKSLQEK 319

BLAST of MS010186 vs. ExPASy Swiss-Prot
Match: Q9SAH9 (Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.3e-73
Identity = 151/316 (47.78%), Postives = 205/316 (64.87%), Query Frame = 0

Query: 7   KTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANL 66
           K VCVTGA GYIASWIVK LL RGYTV+ +VR+P+DP K  HL  L GA ERL L  A+L
Sbjct: 6   KLVCVTGAGGYIASWIVKLLLERGYTVRGTVRNPTDP-KNNHLRELQGAKERLTLHSADL 65

Query: 67  LEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVL 126
           L+  +  + + GC+GVFHTASP    ++D    +++PA+ G   V+ + AK+  VKRVV 
Sbjct: 66  LDYEALCATIDGCDGVFHTASP----MTDDPETMLEPAVNGAKFVIDAAAKAK-VKRVVF 125

Query: 127 TSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDM 186
           TSSI AV Y    R    +VDE  +S  + CK  K WY   K LAE++AW   + KG+D+
Sbjct: 126 TSSIGAV-YMNPNRDTQAIVDENCWSDLDFCKNTKNWYCYGKMLAEQSAWETAKAKGVDL 185

Query: 187 VTINPAMVIGPLLQPTLNTSAEAILNLISG-AQTFPNSTFGWVNVKDVANAHIQAYEIPS 246
           V +NP +V+GP LQ  +N S   IL  ++G A+T+ N T  +V+V+DVA  H+  YE PS
Sbjct: 186 VVLNPVLVLGPPLQSAINASLVHILKYLTGSAKTYANLTQVYVDVRDVALGHVLVYEAPS 245

Query: 247 ASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDK-PFIPTYQVSMEKAKSLGIEFI 306
           ASGRY L E  +H    V+IL   +P   LP KC+D+K P    Y+ + +K K LG+EF 
Sbjct: 246 ASGRYILAETALHRGEVVEILAKFFPEYPLPTKCSDEKNPRAKPYKFTTQKIKDLGLEFK 305

Query: 307 PLEESLKETVESLKEK 321
           P+++SL E+V+SL+EK
Sbjct: 306 PIKQSLYESVKSLQEK 314

BLAST of MS010186 vs. ExPASy Swiss-Prot
Match: Q500U8 (Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 9.1e-72
Identity = 147/318 (46.23%), Postives = 206/318 (64.78%), Query Frame = 0

Query: 9   VCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANLLE 68
           VCVTGASG++ASW+VK LL  GY V  +VRDP +  K AHL  L+GA ERL L KA+L+E
Sbjct: 8   VCVTGASGFLASWLVKRLLLEGYEVIGTVRDPGNEKKLAHLWKLEGAKERLRLVKADLME 67

Query: 69  EGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVLTS 128
           EGSFD+A+ GC+GVFHTASP     S+P+ E++ PA++GTLNVL+S  K+ ++KRVVLTS
Sbjct: 68  EGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSLKRVVLTS 127

Query: 129 SIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDMVT 188
           S + V   +    P + +DE+ ++S E+CK  ++WY LSKTLAE+AAW F  E GID+VT
Sbjct: 128 SSSTVRIRD-DFDPKIPLDESIWTSVELCKRFQVWYALSKTLAEQAAWKFSEENGIDLVT 187

Query: 189 INPAMVIGPLLQPTLNTSAEAILNLISG-AQTFP-NSTFGWVNVKDVANAHIQAYEIPSA 248
           + P+ ++GP L P L ++A  +L L+ G  + F  +   G+V++ DVA  HI  +E  +A
Sbjct: 188 VLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTHIVVFEHEAA 247

Query: 249 SGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEFIPL 308
            GRY     VI     V  L   YPSL +P +   +K     Y     K +SLG++F  L
Sbjct: 248 QGRYICSSNVISLEELVSFLSARYPSLPIPKRF--EKLNRLHYDFDTSKIQSLGLKFKSL 307

Query: 309 EESLKETVESLKEKKFVS 325
           EE   + + SL E+ ++S
Sbjct: 308 EEMFDDCIASLVEQGYLS 322

BLAST of MS010186 vs. ExPASy TrEMBL
Match: A0A6J1DHU3 (cinnamoyl-CoA reductase 1-like OS=Momordica charantia OX=3673 GN=LOC111020626 PE=3 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 7.3e-181
Identity = 325/325 (100.00%), Postives = 325/325 (100.00%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH
Sbjct: 57  MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 116

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST
Sbjct: 117 LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 176

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 177 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 236

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA
Sbjct: 237 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 296

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG
Sbjct: 297 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 356

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEKKFVSF
Sbjct: 357 IEFIPLEESLKETVESLKEKKFVSF 381

BLAST of MS010186 vs. ExPASy TrEMBL
Match: A0A6J1K6Z5 (cinnamoyl-CoA reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490754 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 297/325 (91.38%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGASGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAV+GCEGVFHTASPFFH+VSDPQAELIDPALKGTLNVL SVAK+S+
Sbjct: 61  LFKANLLEEGSFDSAVEGCEGVFHTASPFFHNVSDPQAELIDPALKGTLNVLNSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD VVDETWF+ PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNRQPRNPDTVVDETWFTDPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAIL LISGA+TF NSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILKLISGAETFQNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVE+VIHYSG VKIL DLYPSL+LPDKCADDKP +PTYQVS+EKAK LG
Sbjct: 241 YEIPSASGRYCLVEKVIHYSGIVKILHDLYPSLQLPDKCADDKPQVPTYQVSVEKAKGLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEESLKETVESLKEKKF+SF
Sbjct: 301 IEFIPLEESLKETVESLKEKKFISF 325

BLAST of MS010186 vs. ExPASy TrEMBL
Match: A0A6J1H1P9 (cinnamoyl-CoA reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459327 PE=3 SV=1)

HSP 1 Score: 598.6 bits (1542), Expect = 1.6e-167
Identity = 296/325 (91.08%), Postives = 313/325 (96.31%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSGGAAKTVCVTGASGYIASWIVKFLL+RGYTVKASVRDP+DPIKTAHLLALDGAAERLH
Sbjct: 1   MSGGAAKTVCVTGASGYIASWIVKFLLQRGYTVKASVRDPADPIKTAHLLALDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSAV+GCEGVFHTASPFFH+VSDPQAELIDPALKGTLNVL SVAK+S+
Sbjct: 61  LFKANLLEEGSFDSAVEGCEGVFHTASPFFHNVSDPQAELIDPALKGTLNVLNSVAKASS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD VVDETWF+ PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNRQPRNPDTVVDETWFTDPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDMVTINPAMVIGPLLQPTLNTSAEAIL LISGA+TFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILKLISGAETFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YEIPSASGRYCLVE+VIH+SG VKIL DLYPSL+LPDKCADDKP +PTYQVS+EKAK LG
Sbjct: 241 YEIPSASGRYCLVEKVIHHSGIVKILHDLYPSLRLPDKCADDKPQVPTYQVSVEKAKGLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           IEFIPLEE LKETVESLKEKKF+SF
Sbjct: 301 IEFIPLEEGLKETVESLKEKKFISF 325

BLAST of MS010186 vs. ExPASy TrEMBL
Match: A0A1S3BNJ9 (cinnamoyl-CoA reductase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491499 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.3e-165
Identity = 289/325 (88.92%), Postives = 312/325 (96.00%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSG AAKTVCVTG SGYIASWIVKFLL+RGYTVKASVR+P+DPIKTAHLL+LDGAAERLH
Sbjct: 1   MSGEAAKTVCVTGGSGYIASWIVKFLLQRGYTVKASVRNPADPIKTAHLLSLDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSA++GC+GVFHTASPFFH+VSDPQAELIDPALKGTLNVLKSVAKSS+
Sbjct: 61  LFKANLLEEGSFDSAIEGCQGVFHTASPFFHNVSDPQAELIDPALKGTLNVLKSVAKSSS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD V+DETWFS+PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNGQPRNPDTVIDETWFSNPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDM+TINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMITINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YE+PSA+GRYCLVE VIHYSG VKIL DLYPSL+LPDKCADDKPF P YQVS+EKAK+LG
Sbjct: 241 YEVPSANGRYCLVESVIHYSGIVKILHDLYPSLQLPDKCADDKPFTPVYQVSVEKAKNLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           I+FIPL ESLKETVESLKEK F++F
Sbjct: 301 IQFIPLVESLKETVESLKEKNFINF 325

BLAST of MS010186 vs. ExPASy TrEMBL
Match: A0A5D3B8F2 (Cinnamoyl-CoA reductase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00960 PE=3 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.3e-165
Identity = 289/325 (88.92%), Postives = 312/325 (96.00%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MSG AAKTVCVTG SGYIASWIVKFLL+RGYTVKASVR+P+DPIKTAHLL+LDGAAERLH
Sbjct: 1   MSGEAAKTVCVTGGSGYIASWIVKFLLQRGYTVKASVRNPADPIKTAHLLSLDGAAERLH 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSA++GC+GVFHTASPFFH+VSDPQAELIDPALKGTLNVLKSVAKSS+
Sbjct: 61  LFKANLLEEGSFDSAIEGCQGVFHTASPFFHNVSDPQAELIDPALKGTLNVLKSVAKSSS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSS+AAVAYN +PR PD V+DETWFS+PE+CKELKLWYVLSKTLAEEAAWNFVR
Sbjct: 121 VKRVVLTSSMAAVAYNGQPRNPDTVIDETWFSNPEICKELKLWYVLSKTLAEEAAWNFVR 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           EKGIDM+TINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHI A
Sbjct: 181 EKGIDMITINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHILA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           YE+PSA+GRYCLVE VIHYSG VKIL DLYPSL+LPDKCADDKPF P YQVS+EKAK+LG
Sbjct: 241 YEVPSANGRYCLVESVIHYSGIVKILHDLYPSLQLPDKCADDKPFTPVYQVSVEKAKNLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           I+FIPL ESLKETVESLKEK F++F
Sbjct: 301 IQFIPLVESLKETVESLKEKNFINF 325

BLAST of MS010186 vs. TAIR 10
Match: AT5G19440.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-145
Identity = 244/324 (75.31%), Postives = 286/324 (88.27%), Query Frame = 0

Query: 2   SGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHL 61
           + G  K VCVTGASGYIASW+VKFLL RGYTVKASVRDPSDP KT HL++L+GA ERLHL
Sbjct: 3   NSGEGKVVCVTGASGYIASWLVKFLLSRGYTVKASVRDPSDPKKTQHLVSLEGAKERLHL 62

Query: 62  FKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTV 121
           FKA+LLE+GSFDSA+ GC GVFHTASPFF+   DPQAELIDPA+KGTLNVL S AK+S+V
Sbjct: 63  FKADLLEQGSFDSAIDGCHGVFHTASPFFNDAKDPQAELIDPAVKGTLNVLNSCAKASSV 122

Query: 122 KRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVRE 181
           KRVV+TSS+AAV YN KPRTPDV VDETWFS PE+C+  K+WYVLSKTLAE+AAW   +E
Sbjct: 123 KRVVVTSSMAAVGYNGKPRTPDVTVDETWFSDPELCEASKMWYVLSKTLAEDAAWKLAKE 182

Query: 182 KGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAY 241
           KG+D+VTINPAMVIGPLLQPTLNTSA AILNLI+GA+TFPN +FGWVNVKDVANAHIQA+
Sbjct: 183 KGLDIVTINPAMVIGPLLQPTLNTSAAAILNLINGAKTFPNLSFGWVNVKDVANAHIQAF 242

Query: 242 EIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGI 301
           E+PSA+GRYCLVERV+H+S  V ILR+LYP+L LP++C D+ P++PTYQVS +K +SLGI
Sbjct: 243 EVPSANGRYCLVERVVHHSEIVNILRELYPNLPLPERCVDENPYVPTYQVSKDKTRSLGI 302

Query: 302 EFIPLEESLKETVESLKEKKFVSF 326
           ++IPL+ S+KETVESLKEK F  F
Sbjct: 303 DYIPLKVSIKETVESLKEKGFAQF 326

BLAST of MS010186 vs. TAIR 10
Match: AT1G51410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 504.6 bits (1298), Expect = 5.9e-143
Identity = 244/325 (75.08%), Postives = 279/325 (85.85%), Query Frame = 0

Query: 1   MSGGAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLH 60
           MS    KTVCVTGASGYIASWIVK LL RGYTVKASVRDP+DP KT HLLAL+GA ERL 
Sbjct: 1   MSSEEEKTVCVTGASGYIASWIVKLLLLRGYTVKASVRDPNDPRKTEHLLALEGAEERLK 60

Query: 61  LFKANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSST 120
           LFKANLLEEGSFDSA+ GCEGVFHTASPF+H V DPQAEL+DPA+KGT+NVL S  K+S+
Sbjct: 61  LFKANLLEEGSFDSAIDGCEGVFHTASPFYHDVKDPQAELLDPAVKGTINVLSSCLKTSS 120

Query: 121 VKRVVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVR 180
           VKRVVLTSSIAAVA+N  PRTP+ +VDETWF+ P+ C+  KLWYVLSKTLAE AAW F +
Sbjct: 121 VKRVVLTSSIAAVAFNGMPRTPETIVDETWFADPDYCRASKLWYVLSKTLAENAAWKFAK 180

Query: 181 EKGIDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQA 240
           E  + +V+INPAMVIGPLLQPTLNTSA A+L+LI GAQTFPN+TFGWVNVKDVANAHIQA
Sbjct: 181 ENNLQLVSINPAMVIGPLLQPTLNTSAAAVLSLIKGAQTFPNATFGWVNVKDVANAHIQA 240

Query: 241 YEIPSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLG 300
           +E P A GRYCLVERV HYS  V IL DLYP  +LP+KCAD+K +IPTY+VS EKA+SLG
Sbjct: 241 FENPDADGRYCLVERVAHYSEVVNILHDLYPDFQLPEKCADEKIYIPTYKVSKEKAESLG 300

Query: 301 IEFIPLEESLKETVESLKEKKFVSF 326
           +EF+PLE S+KETVESL++K F+ F
Sbjct: 301 VEFVPLEVSIKETVESLRDKGFIRF 325

BLAST of MS010186 vs. TAIR 10
Match: AT1G09510.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 365.9 bits (938), Expect = 3.3e-101
Identity = 186/317 (58.68%), Postives = 236/317 (74.45%), Query Frame = 0

Query: 7   KTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANL 66
           K VCVTGASGY+ASWIVK LL RGYTV+A+VRDPSD  KT HLLALDGA E+L LFKA+L
Sbjct: 6   KMVCVTGASGYVASWIVKLLLLRGYTVRATVRDPSDEKKTEHLLALDGAKEKLKLFKADL 65

Query: 67  LEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVL 126
           LEEGSF+ A++GC+ VFHTASP   +V+DPQ ELIDPA+KGTLNVLK+ AK S+VKRV++
Sbjct: 66  LEEGSFEQAIEGCDAVFHTASPVSLTVTDPQIELIDPAVKGTLNVLKTCAKVSSVKRVIV 125

Query: 127 TSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDM 186
           TSS+AAV + E    P+ +VDE+ FS P  C E KLWY LSKTLAE+ AW F +EKG+D+
Sbjct: 126 TSSMAAVLFREPTLGPNDLVDESCFSDPNFCTEKKLWYALSKTLAEDEAWRFAKEKGLDL 185

Query: 187 VTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAYEIPSA 246
           V INP +V+GPLL+P+L  S   I+ LI+G   F N  F  V+V+DVA AHI+A+E PSA
Sbjct: 186 VVINPGLVLGPLLKPSLTFSVNVIVELITGKDNFINKDFRLVDVRDVALAHIKAFETPSA 245

Query: 247 SGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEFIPL 306
           +GRY +   V+  +   KILR+ +P L L +K    +     Y++ +EK KSLGIEF P 
Sbjct: 246 NGRYIIEGPVVTINDIEKILREFFPDLNLGNKGEASEIIPVIYKLCVEKVKSLGIEFTPT 305

Query: 307 EESLKETVESLKEKKFV 324
           E +L++T+ SLKEK  V
Sbjct: 306 EATLRDTILSLKEKCLV 322

BLAST of MS010186 vs. TAIR 10
Match: AT1G09480.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 352.1 bits (902), Expect = 4.9e-97
Identity = 179/317 (56.47%), Postives = 229/317 (72.24%), Query Frame = 0

Query: 4   GAAKTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFK 63
           G  K VCVTGASGYIASWIVK LL RGYTVKA+VRD +D  KT HLLALDGA ERL LFK
Sbjct: 50  GGGKLVCVTGASGYIASWIVKLLLLRGYTVKATVRDLTDRKKTEHLLALDGAKERLKLFK 109

Query: 64  ANLLEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKR 123
           A+LLEE SF+ A++GC+ VFHTASP F +V DPQ ELIDPALKGT+NVL +  ++ +V+R
Sbjct: 110 ADLLEESSFEQAIEGCDAVFHTASPVFFTVKDPQTELIDPALKGTMNVLNTCKETPSVRR 169

Query: 124 VVLTSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKG 183
           V+LTSS AAV + + P     VVDET+FS P +C+E K WY LSK LAE AAW F ++ G
Sbjct: 170 VILTSSTAAVLFRQPPVEASDVVDETFFSDPSLCRETKNWYPLSKILAENAAWEFAKDNG 229

Query: 184 IDMVTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAYEI 243
           IDMV +NP  + GPLLQPTLN S E I++ I+G   F +  + +V+V+DVA AHI+A E 
Sbjct: 230 IDMVVLNPGFIFGPLLQPTLNFSVELIVDFINGKNPFNSRFYRFVDVRDVALAHIKALET 289

Query: 244 PSASGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEF 303
           PSA+GRY +   ++  S  + ILR+L P L + D   +        +V +EK K+LG+EF
Sbjct: 290 PSANGRYIIDGPIMSVSDIIDILRELLPDLCIADTNEESVMNEMLCKVCVEKVKNLGVEF 349

Query: 304 IPLEESLKETVESLKEK 321
            P++ SL++T+ SLKEK
Sbjct: 350 TPMKSSLRDTIVSLKEK 366

BLAST of MS010186 vs. TAIR 10
Match: AT1G09490.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 1.9e-96
Identity = 178/314 (56.69%), Postives = 224/314 (71.34%), Query Frame = 0

Query: 7   KTVCVTGASGYIASWIVKFLLRRGYTVKASVRDPSDPIKTAHLLALDGAAERLHLFKANL 66
           K VCVTGASGYIASWIVK LL RGYTV A+VRDP D  KT HLLALDGA ERL LFKA+L
Sbjct: 6   KVVCVTGASGYIASWIVKLLLLRGYTVNATVRDPKDKKKTEHLLALDGAKERLKLFKADL 65

Query: 67  LEEGSFDSAVQGCEGVFHTASPFFHSVSDPQAELIDPALKGTLNVLKSVAKSSTVKRVVL 126
           LEE SFD A+ GC+ VFHTASP   +V+DPQ ELIDPALKGT+NVL +  + S+VKRV+L
Sbjct: 66  LEESSFDQAIDGCDAVFHTASPVLFTVTDPQTELIDPALKGTINVLNTCKQVSSVKRVIL 125

Query: 127 TSSIAAVAYNEKPRTPDVVVDETWFSSPEVCKELKLWYVLSKTLAEEAAWNFVREKGIDM 186
           TSS AAV   + P  P+ +VDET+FS P +C+E K WY LSK LAE AAW F ++ GIDM
Sbjct: 126 TSSTAAVLSRQPPIGPNDLVDETFFSDPSLCRETKNWYSLSKILAENAAWQFAKDNGIDM 185

Query: 187 VTINPAMVIGPLLQPTLNTSAEAILNLISGAQTFPNSTFGWVNVKDVANAHIQAYEIPSA 246
           V +NP  + GPLLQPTLN S E I++ I+G   F    + + +V+DVA  HI+A E PSA
Sbjct: 186 VVLNPGFICGPLLQPTLNMSVELIVDFINGKNPFNKRYYRFSDVRDVALVHIKALETPSA 245

Query: 247 SGRYCLVERVIHYSGTVKILRDLYPSLKLPDKCADDKPFIPTYQVSMEKAKSLGIEFIPL 306
           +GRY +    +  +  + ILR L+P L + D   + +      QV +EK K+LG+EF P+
Sbjct: 246 NGRYIIDGPNMSVNDIIDILRKLFPDLSIADTNEESEMNEMICQVCVEKVKNLGVEFTPM 305

Query: 307 EESLKETVESLKEK 321
           + SL++T+ SLKEK
Sbjct: 306 KSSLRDTIVSLKEK 319

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022153022.11.5e-180100.00cinnamoyl-CoA reductase 1-like [Momordica charantia][more]
XP_038901182.11.0e-16891.38phenylacetaldehyde reductase-like [Benincasa hispida][more]
KAG6605921.11.1e-16791.38Cinnamoyl-CoA reductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_022957943.13.2e-16791.08cinnamoyl-CoA reductase 1-like [Cucurbita moschata][more]
XP_022995113.13.2e-16791.38cinnamoyl-CoA reductase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A0B6VQ484.4e-15180.25Phenylacetaldehyde reductase OS=Rosa hybrid cultivar OX=128735 GN=PAR PE=1 SV=1[more]
A0A059TC024.8e-8150.31Cinnamoyl-CoA reductase 1 OS=Petunia hybrida OX=4102 GN=CCR1 PE=1 SV=1[more]
Q9S9N94.5e-7950.00Cinnamoyl-CoA reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CCR1 PE=1 SV=1[more]
Q9SAH91.3e-7347.78Cinnamoyl-CoA reductase 2 OS=Arabidopsis thaliana OX=3702 GN=CCR2 PE=1 SV=1[more]
Q500U89.1e-7246.23Tetraketide alpha-pyrone reductase 1 OS=Arabidopsis thaliana OX=3702 GN=TKPR1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1DHU37.3e-181100.00cinnamoyl-CoA reductase 1-like OS=Momordica charantia OX=3673 GN=LOC111020626 PE... [more]
A0A6J1K6Z51.6e-16791.38cinnamoyl-CoA reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490754 PE=3 ... [more]
A0A6J1H1P91.6e-16791.08cinnamoyl-CoA reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111459327 PE=... [more]
A0A1S3BNJ97.3e-16588.92cinnamoyl-CoA reductase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349149... [more]
A0A5D3B8F27.3e-16588.92Cinnamoyl-CoA reductase 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT5G19440.12.2e-14575.31NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G51410.15.9e-14375.08NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09510.13.3e-10158.68NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09480.14.9e-9756.47NAD(P)-binding Rossmann-fold superfamily protein [more]
AT1G09490.11.9e-9656.69NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 7..325
e-value: 1.4E-141
score: 473.3
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 3..323
NoneNo IPR availablePANTHERPTHR10366:SF730BIFUNCTIONAL POLYMYXIN RESISTANCE PROTEIN, ARNA-RELATEDcoord: 3..323
NoneNo IPR availableCDDcd08958FR_SDR_ecoord: 9..300
e-value: 9.68104E-152
score: 425.835
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 9..244
e-value: 1.1E-19
score: 70.9
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 7..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010186.1MS010186.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity