MS010158 (gene) Bitter gourd (TR) v1

BLAST of MS010158 vs. NCBI nr
Match: XP_038901515.1 (E3 ubiquitin-protein ligase KEG-like isoform X1 [Benincasa hispida])

HSP 1 Score: 3143.6 bits (8149), Expect = 0.0e+00
Identity = 1510/1624 (92.98%), Postives = 1556/1624 (95.81%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICR HYDEDSRAPLLLQCGHTFC+HCLSQIIAPTPPKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRIHYDEDSRAPLLLQCGHTFCKHCLSQIIAPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVST GNSVLSLPKNFAILPMIS  SVSHSAEVSDSDDDDAGADEDG D+SDRGRR+SGC
Sbjct: 61   HVSTIGNSVLSLPKNFAILPMISSASVSHSAEVSDSDDDDAGADEDGVDESDRGRRSSGC 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
            HGCG GFGDHELKLVRKID GKREE+ELWF WLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  HGCGEGFGDHELKLVRKIDGGKREEMELWFAWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTV-PEQE 300
            GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG   PE E
Sbjct: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPPEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SS+QHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSD WSFGCALVEMCTGS
Sbjct: 301  SSRQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDVWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGLSAEEIYR+VVKE +LPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLSAEEIYRSVVKEGKLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFLRHLQGI RSPT R  E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLRHLQGIHRSPT-RPVEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAIL+YSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNSSSVISLLEAHNSEGQTALHLACRRGSPELVDAILDYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAECVRALIRKSANGMFRLMEGF RSVAHVCAYYGQPDCMRELLRAGAD
Sbjct: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFSRSVAHVCAYYGQPDCMRELLRAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCIT+LNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITSLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELASPEEISEAIDIPS+TGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ
Sbjct: 661  RWAELASPEEISEAIDIPSSTGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGA+C
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNLHNTIPLHLALARGAKPCVQLLLSAGADC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECLECILLILKYP AAIGVRNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPGAAIGVRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMD L EKGIHLSPT FQVGDWVKF+RCVANPAYGW+GAGPRSVGFVQGS S 
Sbjct: 841  REWIFEELMDGLAEKGIHLSPTIFQVGDWVKFKRCVANPAYGWQGAGPRSVGFVQGSHSG 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
            DGLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSIGTVLCIDD
Sbjct: 901  DGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSRDSIGTVLCIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYTVP AKHGFGAVTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTVPAAKHGFGAVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPD SLLIEFCYL SPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDCSLLIEFCYLPSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLLII+LP+R+GPWKVDPSDMEKV+KF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLIIDLPHRNGPWKVDPSDMEKVDKFKVGDWVRVKTSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQE+HILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEVHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGKIERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKIERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNNT +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEK+ P+KIGQYV FRAGLAKP
Sbjct: 1261 SNNTGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKVSPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLK-DD 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLK DD
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKEDD 1380

Query: 1381 TDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVK 1440
            TDGRKSLP GS+GVVQGLSYHENEWDGSVLVGFCREPE+WVGHTSKLEK +RF+IGQ VK
Sbjct: 1381 TDGRKSLPTGSIGVVQGLSYHENEWDGSVLVGFCREPELWVGHTSKLEKMERFYIGQHVK 1440

Query: 1441 VKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNI 1500
            VK SV NPRFGWSGHSHASIVSITAIDADGKIKVS+PSAQKPWTLDPSEV  VEEEQL I
Sbjct: 1441 VKSSVTNPRFGWSGHSHASIVSITAIDADGKIKVSSPSAQKPWTLDPSEVVLVEEEQLKI 1500

Query: 1501 GDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPF 1560
            GDWVKVKPSIVMPVYHWGDV+RQSVGV+HKMEDGELWVAFCF+EQLW+CKDSE+EKVRPF
Sbjct: 1501 GDWVKVKPSIVMPVYHWGDVSRQSVGVIHKMEDGELWVAFCFMEQLWMCKDSEMEKVRPF 1560

Query: 1561 RVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVL 1620
            RVGD VRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRV+FRWREGRPWIGDPADLVL
Sbjct: 1561 RVGDMVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVKFRWREGRPWIGDPADLVL 1620

BLAST of MS010158 vs. NCBI nr
Match: XP_023533534.1 (E3 ubiquitin-protein ligase KEG-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3115.9 bits (8077), Expect = 0.0e+00
Identity = 1493/1623 (91.99%), Postives = 1545/1623 (95.19%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICRTHYDEDSR PLLLQCGHTFC+HCLSQIIAPTPPKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRTHYDEDSRVPLLLQCGHTFCKHCLSQIIAPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVS  GNSVLSLPKNFAILPMISP SVSHSAEVSDSDDDDAG DEDG  +SDRGRR+ G 
Sbjct: 61   HVSAVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDAGGDEDGVGESDRGRRSYGF 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
             GCG GFGDHELKLVRKID  KR E+ELWFGWLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  DGCGAGFGDHELKLVRKIDGEKRVEVELWFGWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVP-EQE 300
            GADIARAVVELHAADVLCM+LKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG  P E E
Sbjct: 241  GADIARAVVELHAADVLCMDLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPQEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSDAWSFGCALVEMCTGS
Sbjct: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDAWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGL+AEEIYRAVVKE RLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLTAEEIYRAVVKEGRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFL+HLQGI RSPT  ++E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLQHLQGIHRSPTKPSSEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNNSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAEC+RALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECLRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCITTLNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITTLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELA PEEISEAIDIPS+TGTALCMAAALKKDRE EGRELVR+LLK+KA+PAAQDPQQ
Sbjct: 661  RWAELALPEEISEAIDIPSSTGTALCMAAALKKDRETEGRELVRILLKSKANPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AA  +RNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAATSIRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL EKGIHLSPT FQVGDWVKF+ CV NPAYGW+GAGPRSVGFVQGS SS
Sbjct: 841  REWIFEELMDALAEKGIHLSPTIFQVGDWVKFKSCVTNPAYGWQGAGPRSVGFVQGSHSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
             GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSI TVL IDD
Sbjct: 901  GGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVKEPRFKLLGQSRDSIATVLYIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYT P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTAPNAKHGFGFVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCYL SPWLCEPEEIE V PFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYLPSPWLCEPEEIELVAPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLL+I+LPNRHGPWKVDPSDMEKVEKF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLLIDLPNRHGPWKVDPSDMEKVEKFKVGDWVRVKGSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFR KTFPCSVTDIEKVPPFEVGQEIHILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRRKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKTERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNN  +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEKIPP+KIGQYV FRAGLAKP
Sbjct: 1261 SNNNGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKIPPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDDT 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMY VGEWVKLKDDT
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYEVGEWVKLKDDT 1380

Query: 1381 DGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVKV 1440
            +GRKSLPAGS+GVVQGLSYHENEWDGSVLVGFCREPE+WVGHTS+LEKTDRF+IGQRVKV
Sbjct: 1381 EGRKSLPAGSIGVVQGLSYHENEWDGSVLVGFCREPELWVGHTSELEKTDRFYIGQRVKV 1440

Query: 1441 KPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNIG 1500
            K SVP+PRFGWSGHSHA+IVSITAIDADGKIKVS+PSAQKPWTLDPSEVD VEEE+LNIG
Sbjct: 1441 KTSVPSPRFGWSGHSHANIVSITAIDADGKIKVSSPSAQKPWTLDPSEVDLVEEEKLNIG 1500

Query: 1501 DWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPFR 1560
            DWVKVKPSIVMPV+HWG+V+RQSVG++HKM+ GELWVAFCF+EQLW+CKD EVEKVRPFR
Sbjct: 1501 DWVKVKPSIVMPVHHWGEVSRQSVGIIHKMDGGELWVAFCFMEQLWMCKDLEVEKVRPFR 1560

Query: 1561 VGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVLD 1620
            VGDAVRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVLD
Sbjct: 1561 VGDAVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVLD 1620

BLAST of MS010158 vs. NCBI nr
Match: XP_008449651.1 (PREDICTED: E3 ubiquitin-protein ligase KEG-like [Cucumis melo])

HSP 1 Score: 3110.1 bits (8062), Expect = 0.0e+00
Identity = 1490/1625 (91.69%), Postives = 1551/1625 (95.45%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICR H+DEDSR+PLLLQCGHTFC+HCLSQIIAPT PKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRIHFDEDSRSPLLLQCGHTFCKHCLSQIIAPTSPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVST GNSVLSLPKNFAILPMISP SVSHSAEV+DSDDDDAGADEDGAD+SDRGRR+SGC
Sbjct: 61   HVSTIGNSVLSLPKNFAILPMISPASVSHSAEVTDSDDDDAGADEDGADESDRGRRSSGC 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
            HGCG GFGDHELKLVRKID GKREE+ELWF WLRSR GGCRHRVV RRV+MGNVGDLDWV
Sbjct: 121  HGCGEGFGDHELKLVRKIDGGKREEMELWFAWLRSRVGGCRHRVVVRRVKMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRAS+WCRNVCSFLGV+KVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASIWCRNVCSFLGVMKVEDYLCIVMDWFPGSVQSEMQRSDGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTV-PEQE 300
            GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG   PE E
Sbjct: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPPEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SS+QHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSD WSFGCALVEMCTGS
Sbjct: 301  SSRQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDVWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGLSAEEIYR+VVKE +LPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLSAEEIYRSVVKEGKLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            +FLRHLQGI R PT  T E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  VFLRHLQGIHRPPTRPTAEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAIL+YSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNSSSVISLLEAHNSEGQTALHLACRRGSPELVDAILDYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCIT+LNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITSLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             W ELASPEEISEAIDIPS+TGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ
Sbjct: 661  RWVELASPEEISEAIDIPSSTGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNI N+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDVELVKIILDAGVDVNITNLHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFH+AADAAKFIRECLECILLILKYP AAIGVRNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHLAADAAKFIRECLECILLILKYPGAAIGVRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL+EKGIHLSPT FQVGDWVKF++CV NPAYGW+GAGPRSVGFVQGSQ S
Sbjct: 841  REWIFEELMDALEEKGIHLSPTIFQVGDWVKFKKCVTNPAYGWQGAGPRSVGFVQGSQGS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
            DGLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+ DSIGTVLCIDD
Sbjct: 901  DGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSPDSIGTVLCIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYT+P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTLPAAKHGFGDVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCY+ SPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYVPSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVS+IESNGLLII+LPNRHGPWKVDPSDMEKV+KF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSEIESNGLLIIDLPNRHGPWKVDPSDMEKVDKFKVGDWVRVKTSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQE+HILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEVHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK+ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVR+KQCLG +SNYE
Sbjct: 1201 TPASSGKVERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRIKQCLGARSNYE 1260

Query: 1261 SNNTVREKIAVVYSI-QDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAK 1320
            SNNT +E IAVVYSI QDYSYLELA CF +GK  VH TEVEKI P+KIGQYV FRAGL K
Sbjct: 1261 SNNTGKENIAVVYSILQDYSYLELAFCF-QGKLVVHFTEVEKISPIKIGQYVHFRAGLTK 1320

Query: 1321 PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDD 1380
            PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLK+D
Sbjct: 1321 PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKED 1380

Query: 1381 -TDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRV 1440
             TDGRKSLPA S+GVVQGLSYHENEWDGSVLV FCREPE+WVGHTSKLEKT+RF+IGQRV
Sbjct: 1381 YTDGRKSLPAESIGVVQGLSYHENEWDGSVLVAFCREPELWVGHTSKLEKTERFYIGQRV 1440

Query: 1441 KVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLN 1500
            KVKPS+PNPRFGWSGHSHASIVSITAIDADGKIKVS+ SAQKPW LDPSEV  VEEEQLN
Sbjct: 1441 KVKPSIPNPRFGWSGHSHASIVSITAIDADGKIKVSSSSAQKPWILDPSEVVMVEEEQLN 1500

Query: 1501 IGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRP 1560
            IGDWVK+KPSIVMP YHWGDV RQSVGV+HKMEDGELWVAFCF+EQLW+CKDSE+EKVRP
Sbjct: 1501 IGDWVKIKPSIVMPAYHWGDVARQSVGVIHKMEDGELWVAFCFMEQLWMCKDSEMEKVRP 1560

Query: 1561 FRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLV 1620
            FRVGD VRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLV
Sbjct: 1561 FRVGDRVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLV 1620

BLAST of MS010158 vs. NCBI nr
Match: XP_022958181.1 (E3 ubiquitin-protein ligase KEG-like [Cucurbita moschata] >KAG6605941.1 E3 ubiquitin-protein ligase KEG, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3108.2 bits (8057), Expect = 0.0e+00
Identity = 1490/1623 (91.81%), Postives = 1543/1623 (95.07%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            M KKWKRVGIPSCSICRTHYDEDSR PLLLQCGHTFC+HCLSQIIAPTPPKPSL CPKCR
Sbjct: 1    MAKKWKRVGIPSCSICRTHYDEDSRVPLLLQCGHTFCKHCLSQIIAPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVS  GNSVLSLPKNFAILPMISP SVSHSAEVSDSDDDDAG DEDG  +SDRGRR+ G 
Sbjct: 61   HVSAVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDAGGDEDGVGESDRGRRSYGF 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
             GCG GFGDHELKLVRKID  KR E+ELWFGWLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  DGCGAGFGDHELKLVRKIDGEKRVEVELWFGWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVP-EQE 300
            GADIARAVVELHAADVLCM+LKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG  P E E
Sbjct: 241  GADIARAVVELHAADVLCMDLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPQEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSDAWSFGCALVEMCTGS
Sbjct: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDAWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGL+AEEIYRAVVKE RLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLTAEEIYRAVVKEGRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFL+HLQGI RSPT  ++E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLQHLQGIHRSPTKPSSEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNNSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAEC+RALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECLRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCITTLNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITTLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELA PEEISEAIDIPS+TGTALCMAAALKKDRE EGRELVR+LLK+KA+PAAQDPQQ
Sbjct: 661  RWAELALPEEISEAIDIPSSTGTALCMAAALKKDRETEGRELVRILLKSKANPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AA  +RNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAATSIRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL EKGIHLSPT FQVGDWVKF+ CV NPAYGW+GA PRSVGFVQGS SS
Sbjct: 841  REWIFEELMDALAEKGIHLSPTIFQVGDWVKFKSCVTNPAYGWQGAVPRSVGFVQGSHSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
             GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSI TVL IDD
Sbjct: 901  GGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVKEPRFKLLGQSRDSIATVLYIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYT P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTAPNAKHGFGFVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCYL SPWLCEPEEIE V PFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYLPSPWLCEPEEIELVAPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLL+I+LPNRHGPWKVDPSDMEKVEKF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLLIDLPNRHGPWKVDPSDMEKVEKFKVGDWVRVKGSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFR KTFPCSVTDIEKVPPFEVGQEIHILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRRKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKTERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNN  +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEKIPP+KIGQYV FRAGLAKP
Sbjct: 1261 SNNNGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKIPPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDDT 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMY VGEWVKLKDD 
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYEVGEWVKLKDDA 1380

Query: 1381 DGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVKV 1440
            +GRKSLPAGS+GVVQGLSYHENEWDGSVLVGFCREPE+WVGHTS+LEKTDRF+IGQRVKV
Sbjct: 1381 EGRKSLPAGSIGVVQGLSYHENEWDGSVLVGFCREPELWVGHTSELEKTDRFYIGQRVKV 1440

Query: 1441 KPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNIG 1500
            K SVP+PRFGWSGHSHA+IVSITAIDADGKIKVS+PSAQKPWTLDPSEVD VEEE+LNIG
Sbjct: 1441 KTSVPSPRFGWSGHSHANIVSITAIDADGKIKVSSPSAQKPWTLDPSEVDLVEEEKLNIG 1500

Query: 1501 DWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPFR 1560
            DWVKVKPSIVMPV+HWG+V+R+SVG++HKM+ GELWVAFCF+EQLW+CKDSEVEKVRPFR
Sbjct: 1501 DWVKVKPSIVMPVHHWGEVSRRSVGIIHKMDGGELWVAFCFMEQLWMCKDSEVEKVRPFR 1560

Query: 1561 VGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVLD 1620
            VGDAVRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVLD
Sbjct: 1561 VGDAVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVLD 1620

BLAST of MS010158 vs. NCBI nr
Match: XP_022995009.1 (E3 ubiquitin-protein ligase KEG-like [Cucurbita maxima])

HSP 1 Score: 3107.4 bits (8055), Expect = 0.0e+00
Identity = 1489/1623 (91.74%), Postives = 1541/1623 (94.95%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICRTHYDEDSR PLLLQCGHTFC+HCLSQII PTPPKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRTHYDEDSRVPLLLQCGHTFCKHCLSQIIVPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVS  GNSVLSLPKNFAILPMISP SVSHSAEVSDSDDDDAG DEDG   SDRGRR+ G 
Sbjct: 61   HVSAVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDAGGDEDGVGDSDRGRRSYGF 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
             GCG GFGDHELKLVRKID  KR E+ELWFGWLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  DGCGAGFGDHELKLVRKIDGEKRVEVELWFGWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVP-EQE 300
            GADIARAVVELHAADVLCM+LKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG  P E E
Sbjct: 241  GADIARAVVELHAADVLCMDLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPQEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSDAWSFGCALVEMCTGS
Sbjct: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDAWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGL+AEEIYRAVVKE RLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLTAEEIYRAVVKEGRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFL+HLQGI RSPT  ++E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLQHLQGIHRSPTKPSSEVVSSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNNSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAEC+RALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECLRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCITTLNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITTLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELA PEEISEAIDIPS+TGTALCMAAALKKDRE EGRELVR+LLK+KA+PAAQDPQQ
Sbjct: 661  RWAELALPEEISEAIDIPSSTGTALCMAAALKKDRETEGRELVRILLKSKANPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AA  +RNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAATSIRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL EKGIHLSPT FQVGDWVKF+ CV NPAYGW+GAGPRSVGFVQGS SS
Sbjct: 841  REWIFEELMDALAEKGIHLSPTIFQVGDWVKFKSCVTNPAYGWQGAGPRSVGFVQGSHSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
             GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSI TVL IDD
Sbjct: 901  GGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSRDSIATVLYIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYTV  AKHGFG+VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTVSNAKHGFGSVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCYL SPWLCEPEEIE V PFKIGDQVC+KRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYLPSPWLCEPEEIELVAPFKIGDQVCIKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLL+I+LPNRHGPWKVDPSDMEKVEKF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLLIDLPNRHGPWKVDPSDMEKVEKFKVGDWVRVKGSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFR KTFPCSVTDIEKVPPFEVGQEIHILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRRKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKTERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNN  +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEKIPP+KIGQYV FRAGLAKP
Sbjct: 1261 SNNNGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKIPPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDDT 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMY VGEWVKLKDDT
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYEVGEWVKLKDDT 1380

Query: 1381 DGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVKV 1440
            +GRKSLPAGS+GVVQG SYHENEWDGSVLVGFCREPE+WVGHTS+LEKTDRF++GQRVKV
Sbjct: 1381 EGRKSLPAGSIGVVQGSSYHENEWDGSVLVGFCREPELWVGHTSELEKTDRFYVGQRVKV 1440

Query: 1441 KPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNIG 1500
            K SV NPRF WSGHSHA IVSITAIDADGKIKVS+PSAQKPWTLDPSEVD VEEE+LNIG
Sbjct: 1441 KTSVSNPRFRWSGHSHADIVSITAIDADGKIKVSSPSAQKPWTLDPSEVDLVEEEKLNIG 1500

Query: 1501 DWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPFR 1560
            DWVKVKPSIVMPV+HWG+V+RQSVG++HKM+ GELWVAFCF+EQLW+CKDSEVEKVRPFR
Sbjct: 1501 DWVKVKPSIVMPVHHWGEVSRQSVGIIHKMDGGELWVAFCFMEQLWMCKDSEVEKVRPFR 1560

Query: 1561 VGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVLD 1620
            VGDAVRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVLD
Sbjct: 1561 VGDAVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVLD 1620

BLAST of MS010158 vs. ExPASy Swiss-Prot
Match: Q9FY48 (E3 ubiquitin-protein ligase KEG OS=Arabidopsis thaliana OX=3702 GN=KEG PE=1 SV=2)

HSP 1 Score: 2131.3 bits (5521), Expect = 0.0e+00
Identity = 1006/1632 (61.64%), Postives = 1275/1632 (78.12%), Query Frame = 0

Query: 7    RVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCRHVSTAG 66
            RV +P CS+C T Y+ED R PLLLQCGH FC+ CLS++ + T    +L CP+CRHVS  G
Sbjct: 4    RVKVPCCSVCHTRYNEDERVPLLLQCGHGFCKDCLSKMFS-TSSDTTLTCPRCRHVSVVG 63

Query: 67   NSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGCHG---- 126
            NSV  L KN+A+L +I   S   + +   +DD+D   +EDG+D+ D  R   G H     
Sbjct: 64   NSVQGLRKNYAMLALIHAASGGANFDCDYTDDEDDDDEEDGSDE-DGARAARGFHASSSI 123

Query: 127  ---CG--VGFGDH-ELKLVRKIDE----GKREELELWFGWLRSRGGGCRHRVVARRVRMG 186
               CG  +  G H E+KLVR+I E    G    +E+W   +   GG C+HRV  +++ + 
Sbjct: 124  NSLCGPVIEVGAHPEMKLVRQIGEESSSGGFGGVEMWDATVAGGGGRCKHRVAVKKMTLT 183

Query: 187  NVGDLDWVEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RL 246
               D++W++ QLE LRRASMWCRNVC+F GV+K++  LC++MDR  GSVQSEMQR+  RL
Sbjct: 184  EDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSVQSEMQRNEGRL 243

Query: 247  TLEQILRFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKP-CHR 306
            TLEQILR+GAD+AR V ELHAA V+CMN+KPSN LLDA+G+AVVSDYGL  ILKKP C +
Sbjct: 244  TLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYGLAPILKKPTCQK 303

Query: 307  AGTVPEQESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCA 366
              T PE +SSK     +C+ LSPHY +PEAW P+K+   LF +D  G+S +SDAWSFGC 
Sbjct: 304  --TRPEFDSSKVTLYTDCVTLSPHYTAPEAWGPVKK---LFWEDASGVSPESDAWSFGCT 363

Query: 367  LVEMCTGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKR 426
            LVEMCTGSTPW GLS EEI++AVVK  ++PPQY  IVGVGIPRELWKMIGECLQ+KP KR
Sbjct: 364  LVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGECLQFKPSKR 423

Query: 427  PTFHAMLAIFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSV-LDILQVKSNHLHQLV 486
            PTF+AMLA FLRHLQ IPRSP+              + Q+P +  + + Q   N+LH++V
Sbjct: 424  PTFNAMLATFLRHLQEIPRSPSASPDNGIAKICEVNIVQAPRATNIGVFQDNPNNLHRVV 483

Query: 487  SEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDA 546
             EGD  GVR++L+K+A+G   SSV SLLEA N++GQ+ALHLACRRGS ELV+AILEY +A
Sbjct: 484  LEGDFEGVRNILAKAAAGGGGSSVRSLLEAQNADGQSALHLACRRGSAELVEAILEYGEA 543

Query: 547  DIDSPDENGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCM 606
            ++D  D++G+PP+VFALA GS +CV  LI+K AN   RL EG G SVAHVC+Y+GQPDCM
Sbjct: 544  NVDIVDKDGDPPLVFALAAGSPQCVHVLIKKGANVRSRLREGSGPSVAHVCSYHGQPDCM 603

Query: 607  RELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCI 666
            RELL AGADPNAVDD GE+VLH A+AKK+T CAIVI+E+GG RSM   N+K LTPLHMC+
Sbjct: 604  RELLVAGADPNAVDDEGETVLHRAVAKKYTDCAIVILENGGSRSMTVSNAKCLTPLHMCV 663

Query: 667  TTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKA 726
             T NV V+K W E++SPEEIS+AI+IPS  GTALCMAA+++KD E EGRELV++LL A A
Sbjct: 664  ATWNVAVIKRWVEVSSPEEISQAINIPSPVGTALCMAASIRKDHEKEGRELVQILLAAGA 723

Query: 727  DPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQ 786
            DP AQD Q  RT LHTAAMAN+ ELV++ILDAGV+ NIRNVHNTIPLH+ALARGA  CV 
Sbjct: 724  DPTAQDAQHGRTALHTAAMANNVELVRVILDAGVNANIRNVHNTIPLHMALARGANSCVS 783

Query: 787  LLLSAGANCNLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKT 846
            LLL +G++CN+QDD+GDNAFHIAADAAK IRE L+ ++++L+ PDAA+ VRNH    GKT
Sbjct: 784  LLLESGSDCNIQDDEGDNAFHIAADAAKMIRENLDWLIVMLRSPDAAVDVRNHS---GKT 843

Query: 847  FCDLLEALPREWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSV 906
              D LEALPREWI E+LM+AL ++G+HLSPT ++VGDWVKF+R +  P +GW+GA P+SV
Sbjct: 844  VRDFLEALPREWISEDLMEALLKRGVHLSPTIYEVGDWVKFKRGITTPLHGWQGAKPKSV 903

Query: 907  GFVQGSQSSDGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDS 966
            GFVQ     + + ++FCSG A VLA+E++K+IPLDRGQ V+LR DV EPRF   GQ+RDS
Sbjct: 904  GFVQTILEKEDMIIAFCSGEARVLANEVVKLIPLDRGQHVRLRADVKEPRFGWRGQSRDS 963

Query: 967  IGTVLCIDDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAV 1026
            +GTVLC+ DE+GI+R+GF GASRGW+ADPA+ +R++EFKVGDWVR+R  + +AKHGFG+V
Sbjct: 964  VGTVLCV-DEDGILRVGFPGASRGWKADPAEMERVEEFKVGDWVRIRQNLTSAKHGFGSV 1023

Query: 1027 TPGSIGVVYGIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPW 1086
             PGS+G+VY +RPDSSLL+E  YL +PW CEPEE+EPV PF+IGD+VCVKRS++EPR+ W
Sbjct: 1024 VPGSMGIVYCVRPDSSLLVELSYLPNPWHCEPEEVEPVAPFRIGDRVCVKRSVAEPRYAW 1083

Query: 1087 DGETHNSVGKVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPK 1146
             GETH+SVGK+S+IE++GLLIIE+PNR  PW+ DPSDMEK++ F+VGDWVRVKASV SPK
Sbjct: 1084 GGETHHSVGKISEIENDGLLIIEIPNRPIPWQADPSDMEKIDDFKVGDWVRVKASVSSPK 1143

Query: 1147 YGWEDVPRNSIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVT 1206
            YGWED+ RNSIG++ SL+EDGDV +AFCFRSK F CSVTD+EKV PF VGQEIH+ PS+T
Sbjct: 1144 YGWEDITRNSIGVMHSLDEDGDVGIAFCFRSKPFSCSVTDVEKVTPFHVGQEIHMTPSIT 1203

Query: 1207 QPSLGWLDETPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQ 1266
            QP LGW +ETPA+ GK+ RIDMDGTL+ +V+G++ LWRV+PGDAE LSG  VGDWVR K 
Sbjct: 1204 QPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELLSGFEVGDWVRSKP 1263

Query: 1267 CLGTKSNYESNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYV 1326
             LG + +Y+ +N  RE IAVV+SIQ+  YLELACCF +G++  H T++EKIP LK+GQ+V
Sbjct: 1264 SLGNRPSYDWSNVGRESIAVVHSIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFV 1323

Query: 1327 RFRAGLAKPRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVG 1386
             F+ G+ +PRWGWR A P+SRG++T V+A+GE+RV+ FGL G WRGDPAD EVE M+ VG
Sbjct: 1324 HFQKGITEPRWGWRAAKPDSRGIITTVHADGEVRVAFFGLPGLWRGDPADLEVEPMFEVG 1383

Query: 1387 EWVKLKDDTDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDR 1446
            EWV+L++     KS+  GSVGVV G+ Y  +EWDG+  V FC E E W G TS LEK  +
Sbjct: 1384 EWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAKK 1443

Query: 1447 FHIGQRVKVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDP 1506
              +GQ+ +VK +V  PRFGWSGHSH S+ +I+AIDADGK+++ TP+  K W LDPSEV+ 
Sbjct: 1444 LVVGQKTRVKLAVKQPRFGWSGHSHGSVGTISAIDADGKLRIYTPAGSKTWMLDPSEVET 1503

Query: 1507 VEEEQLNIGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDS 1566
            +EEE+L IGDWV+VK SI  P Y WG+V   S GVVH+MEDG+L V+FCF+++LW+CK  
Sbjct: 1504 IEEEELKIGDWVRVKASITTPTYQWGEVNPSSTGVVHRMEDGDLCVSFCFLDRLWLCKAG 1563

Query: 1567 EVEKVRPFRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWI 1621
            E+E++RPFR+GD V+ ++GL  PRWGWGMETHAS+G VVGVDAN K+R++F WREGRPWI
Sbjct: 1564 ELERIRPFRIGDRVKIKDGLVTPRWGWGMETHASKGHVVGVDANGKLRIKFLWREGRPWI 1623

BLAST of MS010158 vs. ExPASy Swiss-Prot
Match: Q8BZ25 (Ankyrin repeat and protein kinase domain-containing protein 1 OS=Mus musculus OX=10090 GN=Ankk1 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-25
Identity = 155/649 (23.88%), Postives = 256/649 (39.45%), Query Frame = 0

Query: 185 EKLRRASMWCRNVCSFLGVLKVEDHLCIVMD-RFPGSVQSEMQRSRLTLEQILRFGADIA 244
           E ++   +  +++ S  GV K    L IVM+    GS++  +    L     LR   + +
Sbjct: 83  EAVKMEKIKFQHIVSIYGVCK--QPLGIVMEFMASGSLEKTLPTHSLCWPLKLRIIHETS 142

Query: 245 RAVVELHA--ADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVPEQESSKQ 304
            A+  LH+    +L ++LKP N LLD N H  +SD+GL   +           EQ + KQ
Sbjct: 143 LAMNFLHSIKPPLLHLDLKPGNILLDNNMHVKISDFGLSKWM-----------EQSTQKQ 202

Query: 305 HWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGSTPWA 364
           +     L  +  Y  PE          +F ++      + D +SF   + E+ T   P+A
Sbjct: 203 YIERSALRGTLSYIPPE----------MFLENNKAPGPEYDVYSFAIVIWEILTQKKPYA 262

Query: 365 GLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTF-------HA 424
           GL+   I   V    R   Q  S        ++  ++  C    P KRP F         
Sbjct: 263 GLNMMTIIIRVAAGMRPSLQDVSDEWPEEVHQMVNLMKRCWDQDPKKRPCFLNVAVETDM 322

Query: 425 MLAIFLRHL---------QGIPRSPT---------------GRTTEAPCFHHIDRLEQSP 484
           +L++F   +         Q +   P+                 +  +     I +L  S 
Sbjct: 323 LLSLFQSPMTDPGCEALTQKVSCKPSLSQPHKVSKEVNQEIADSVSSDSLKWILQLSDSK 382

Query: 485 TSVL-DILQVKSNHLHQLVSEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHL 544
           + V  D+ + +   LH LV+ G +  VR LLS              ++   + G T L +
Sbjct: 383 SLVASDVYENRVTPLHFLVAGGSLEQVRLLLSHDVD----------VDCQTASGYTPLLI 442

Query: 545 ACRRGSPELVDAILEYSDADIDSPDENGNPPIVFALAVGSAECVRALIRKSANGMFRLME 604
           A +   P+L   +L +  AD +  DE+G  P+ FA   G     R L+   A  +    E
Sbjct: 443 ATQDQQPDLCALLLAHG-ADTNLADEDGWAPLHFAAQNGDDHTARLLLDHGA--LVNARE 502

Query: 605 GFGRSVAHVCAYYGQPDCMRELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGG 664
             G +  H+ A     +  R L+   AD +  +  G++ LHV  A  F H  +V +  G 
Sbjct: 503 HEGWTPLHLAAQNNFENVARLLVSRQADLSPHEAEGKTPLHV--AAYFGHIGLVKLLSGQ 562

Query: 665 CRSMGFLNSKNLTPLHMCITTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALK 724
              +        TPLH+ +    V  ++    L     + +A+D   +  + L +AAA  
Sbjct: 563 GAELDAQQRNLRTPLHLAVERGKVRAIQ---HLLKCGALPDALD--HSGYSPLHIAAA-- 622

Query: 725 KDREIEGRELVRVLLKAKADPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNV 784
                 G++L+  +L           QQ  T LH A      E++  +  + VD++    
Sbjct: 623 -----RGKDLIFKMLLRYGASLELRTQQGWTPLHLATYKGHLEIIHQLAKSHVDLDALGS 681

Query: 785 HNTIPLHLALARGAKPCVQLLLSAGANCNLQDDDGDNAFHIAADAAKFI 799
               PLHLA  +G +  +  LL  GAN N  +  G    H+A     F+
Sbjct: 683 MQWTPLHLAAFQGEEGVMLALLQCGANPNAAEQSGWTPLHLAVHKGTFL 681

BLAST of MS010158 vs. ExPASy Swiss-Prot
Match: C7B178 (Protein VAPYRIN OS=Petunia hybrida OX=4102 GN=VPY PE=2 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 2.7e-23
Identity = 102/356 (28.65%), Postives = 170/356 (47.75%), Query Frame = 0

Query: 452 SVLDILQVKSNHLHQLVSEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLAC 511
           S + I+ V S  L  LV +G ++ +R++L KS       +    +++ N EGQT LHLA 
Sbjct: 128 SAIKIMFVGSPVLCYLVRKGYMDEIREVLEKS------DTTWKSVDSVNFEGQTLLHLAI 187

Query: 512 RRGSPELVDAILEYSDADIDSPDENGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGF 571
            +G P+LV  +LE+   +I++   + + P+  A A G A  V  L+ K A+      E  
Sbjct: 188 SQGRPDLVQLLLEFG-PNIEAHSRSCSSPLEAASATGEALIVELLLAKKAS--TERTEFS 247

Query: 572 GRSVAHVCAYYGQPDCMRELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCR 631
                H+ A  G  + ++ LL  GA+ N++  +G + LH+A+ ++   CA +++ + G R
Sbjct: 248 ASGPIHLAAGNGHLEVLKLLLLKGANVNSLTKDGNTALHLAVEERRRDCARLLLAN-GAR 307

Query: 632 SMGFLNSKNLTPLHMCITTLNVDVVKMWAELASPEEI-----SEAIDIPS---------- 691
           +         TPLH+     +  +V++  +  + + I       A D+ +          
Sbjct: 308 ADICSTGNGDTPLHIAAGLGDEHMVRVLLQKGAEKYIRNKYGKTAYDVAAEHGHNKLFDA 367

Query: 692 -ATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQCRTVLHTAAMANDAELVK 751
              G +LC+AA     R+ E R + R LL+  A    +D Q   T LH A      E+VK
Sbjct: 368 LRLGDSLCVAA-----RKGEVRTVQR-LLENGASINGRD-QHGWTALHRACFKGRIEVVK 427

Query: 752 IILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANCNLQDDDGDNAFHIA 792
            ++D G+DVN R+      LH A+  G     +LL+  GA+  L+   G  A  IA
Sbjct: 428 ALIDNGIDVNARDEDGYTALHCAVESGHVDVAELLVKKGADIELRTSKGITALQIA 466

BLAST of MS010158 vs. ExPASy Swiss-Prot
Match: Q5F478 (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Gallus gallus OX=9031 GN=ANKRD44 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.8e-20
Identity = 98/372 (26.34%), Postives = 162/372 (43.55%), Query Frame = 0

Query: 464 LHQLVSEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAIL 523
           LH   S G +N V+ LL+              ++  N  G TALH+AC  G   +V+ ++
Sbjct: 210 LHAAASNGQINIVKHLLNLGVE----------IDEMNIYGNTALHIACYNGQDSVVNELI 269

Query: 524 EYSDADIDSPDENGNPPIVFALA-VGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYY 583
           +Y  A+++ P+ NG  P+ FA A    A C+  L+   A+    +    G+S  H+ A +
Sbjct: 270 DYG-ANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGAD--VNIQSKDGKSPLHMTAVH 329

Query: 584 GQPDCMRELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLT 643
           G+    + L++ G + + VD +G + LHV  A ++ H  ++                N+ 
Sbjct: 330 GRFTRSQTLIQNGGEIDCVDKDGNTPLHV--AARYGHELLINTLITSGADTAKCGIHNMF 389

Query: 644 PLHMCITTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRV 703
           PLH+     + D  +    L+S  E    ID P + G     AAA   +      E +++
Sbjct: 390 PLHLAALNAHSDCCRKL--LSSGFE----IDTPDSFGRTCLHAAAAGGN-----VECIKL 449

Query: 704 LLKAKADPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALA-- 763
           L  + AD   +D ++ RT LH AA       ++ ++  G ++N  +     PLH A A  
Sbjct: 450 LQSSGADFNKKD-KRGRTPLHYAAANCHFHCIETLVTTGANINETDDWGRTPLHYAAASD 509

Query: 764 --------------------------RGAKPCVQLLLSAGANCNLQDDDGDNAFHIAADA 807
                                     + A  C++ LL   AN ++QD +G N  H A  A
Sbjct: 510 MDRKKNILGNSHENAEELERTSEMKEKEAALCLEFLLQNDANPSIQDKEGYNTVHYA--A 552

BLAST of MS010158 vs. ExPASy Swiss-Prot
Match: Q8N8A2 (Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit B OS=Homo sapiens OX=9606 GN=ANKRD44 PE=1 SV=3)

HSP 1 Score: 101.7 bits (252), Expect = 8.1e-20
Identity = 86/330 (26.06%), Postives = 139/330 (42.12%), Query Frame = 0

Query: 464 LHQLVSEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAIL 523
           LH   S G +N V+ LL+              ++  N  G TALH+AC  G   +V+ ++
Sbjct: 210 LHAAASNGQINVVKHLLNLGVE----------IDEINVYGNTALHIACYNGQDAVVNELI 269

Query: 524 EYSDADIDSPDENGNPPIVFALA-VGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYY 583
           +Y  A+++ P+ NG  P+ FA A    A C+  L+   A+    +    G+S  H+ A +
Sbjct: 270 DYG-ANVNQPNNNGFTPLHFAAASTHGALCLELLVNNGAD--VNIQSKDGKSPLHMTAVH 329

Query: 584 GQPDCMRELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLT 643
           G+    + L++ G + + VD +G + LHVA           ++  G   +   ++S  + 
Sbjct: 330 GRFTRSQTLIQNGGEIDCVDKDGNTPLHVAARYGHELLINTLITSGADTAKCGIHS--MF 389

Query: 644 PLHMCITTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRV 703
           PLH+     + D  +    L+S  E    ID P   G                       
Sbjct: 390 PLHLAALNAHSDCCRKL--LSSGFE----IDTPDKFG----------------------- 449

Query: 704 LLKAKADPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARG 763
                           RT LH AA   + E +K++  +G D + ++     PLH A A  
Sbjct: 450 ----------------RTCLHAAAAGGNVECIKLLQSSGADFHKKDKCGRTPLHYAAANC 479

Query: 764 AKPCVQLLLSAGANCNLQDDDGDNAFHIAA 793
              C++ L++ GAN N  DD G  A H AA
Sbjct: 510 HFHCIETLVTTGANVNETDDWGRTALHYAA 479

BLAST of MS010158 vs. ExPASy TrEMBL
Match: A0A1S3BLW0 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491469 PE=4 SV=1)

HSP 1 Score: 3110.1 bits (8062), Expect = 0.0e+00
Identity = 1490/1625 (91.69%), Postives = 1551/1625 (95.45%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICR H+DEDSR+PLLLQCGHTFC+HCLSQIIAPT PKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRIHFDEDSRSPLLLQCGHTFCKHCLSQIIAPTSPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVST GNSVLSLPKNFAILPMISP SVSHSAEV+DSDDDDAGADEDGAD+SDRGRR+SGC
Sbjct: 61   HVSTIGNSVLSLPKNFAILPMISPASVSHSAEVTDSDDDDAGADEDGADESDRGRRSSGC 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
            HGCG GFGDHELKLVRKID GKREE+ELWF WLRSR GGCRHRVV RRV+MGNVGDLDWV
Sbjct: 121  HGCGEGFGDHELKLVRKIDGGKREEMELWFAWLRSRVGGCRHRVVVRRVKMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRAS+WCRNVCSFLGV+KVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASIWCRNVCSFLGVMKVEDYLCIVMDWFPGSVQSEMQRSDGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTV-PEQE 300
            GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG   PE E
Sbjct: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPPEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SS+QHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSD WSFGCALVEMCTGS
Sbjct: 301  SSRQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDVWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGLSAEEIYR+VVKE +LPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLSAEEIYRSVVKEGKLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            +FLRHLQGI R PT  T E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  VFLRHLQGIHRPPTRPTAEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAIL+YSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNSSSVISLLEAHNSEGQTALHLACRRGSPELVDAILDYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCIT+LNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITSLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             W ELASPEEISEAIDIPS+TGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ
Sbjct: 661  RWVELASPEEISEAIDIPSSTGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNI N+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDVELVKIILDAGVDVNITNLHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFH+AADAAKFIRECLECILLILKYP AAIGVRNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHLAADAAKFIRECLECILLILKYPGAAIGVRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL+EKGIHLSPT FQVGDWVKF++CV NPAYGW+GAGPRSVGFVQGSQ S
Sbjct: 841  REWIFEELMDALEEKGIHLSPTIFQVGDWVKFKKCVTNPAYGWQGAGPRSVGFVQGSQGS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
            DGLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+ DSIGTVLCIDD
Sbjct: 901  DGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSPDSIGTVLCIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYT+P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTLPAAKHGFGDVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCY+ SPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYVPSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVS+IESNGLLII+LPNRHGPWKVDPSDMEKV+KF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSEIESNGLLIIDLPNRHGPWKVDPSDMEKVDKFKVGDWVRVKTSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQE+HILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEVHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK+ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVR+KQCLG +SNYE
Sbjct: 1201 TPASSGKVERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRIKQCLGARSNYE 1260

Query: 1261 SNNTVREKIAVVYSI-QDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAK 1320
            SNNT +E IAVVYSI QDYSYLELA CF +GK  VH TEVEKI P+KIGQYV FRAGL K
Sbjct: 1261 SNNTGKENIAVVYSILQDYSYLELAFCF-QGKLVVHFTEVEKISPIKIGQYVHFRAGLTK 1320

Query: 1321 PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDD 1380
            PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLK+D
Sbjct: 1321 PRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKED 1380

Query: 1381 -TDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRV 1440
             TDGRKSLPA S+GVVQGLSYHENEWDGSVLV FCREPE+WVGHTSKLEKT+RF+IGQRV
Sbjct: 1381 YTDGRKSLPAESIGVVQGLSYHENEWDGSVLVAFCREPELWVGHTSKLEKTERFYIGQRV 1440

Query: 1441 KVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLN 1500
            KVKPS+PNPRFGWSGHSHASIVSITAIDADGKIKVS+ SAQKPW LDPSEV  VEEEQLN
Sbjct: 1441 KVKPSIPNPRFGWSGHSHASIVSITAIDADGKIKVSSSSAQKPWILDPSEVVMVEEEQLN 1500

Query: 1501 IGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRP 1560
            IGDWVK+KPSIVMP YHWGDV RQSVGV+HKMEDGELWVAFCF+EQLW+CKDSE+EKVRP
Sbjct: 1501 IGDWVKIKPSIVMPAYHWGDVARQSVGVIHKMEDGELWVAFCFMEQLWMCKDSEMEKVRP 1560

Query: 1561 FRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLV 1620
            FRVGD VRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLV
Sbjct: 1561 FRVGDRVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLV 1620

BLAST of MS010158 vs. ExPASy TrEMBL
Match: A0A6J1H1F3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111459486 PE=4 SV=1)

HSP 1 Score: 3108.2 bits (8057), Expect = 0.0e+00
Identity = 1490/1623 (91.81%), Postives = 1543/1623 (95.07%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            M KKWKRVGIPSCSICRTHYDEDSR PLLLQCGHTFC+HCLSQIIAPTPPKPSL CPKCR
Sbjct: 1    MAKKWKRVGIPSCSICRTHYDEDSRVPLLLQCGHTFCKHCLSQIIAPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVS  GNSVLSLPKNFAILPMISP SVSHSAEVSDSDDDDAG DEDG  +SDRGRR+ G 
Sbjct: 61   HVSAVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDAGGDEDGVGESDRGRRSYGF 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
             GCG GFGDHELKLVRKID  KR E+ELWFGWLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  DGCGAGFGDHELKLVRKIDGEKRVEVELWFGWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVP-EQE 300
            GADIARAVVELHAADVLCM+LKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG  P E E
Sbjct: 241  GADIARAVVELHAADVLCMDLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPQEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSDAWSFGCALVEMCTGS
Sbjct: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDAWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGL+AEEIYRAVVKE RLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLTAEEIYRAVVKEGRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFL+HLQGI RSPT  ++E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLQHLQGIHRSPTKPSSEVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNNSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAEC+RALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECLRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCITTLNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITTLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELA PEEISEAIDIPS+TGTALCMAAALKKDRE EGRELVR+LLK+KA+PAAQDPQQ
Sbjct: 661  RWAELALPEEISEAIDIPSSTGTALCMAAALKKDRETEGRELVRILLKSKANPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AA  +RNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAATSIRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL EKGIHLSPT FQVGDWVKF+ CV NPAYGW+GA PRSVGFVQGS SS
Sbjct: 841  REWIFEELMDALAEKGIHLSPTIFQVGDWVKFKSCVTNPAYGWQGAVPRSVGFVQGSHSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
             GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSI TVL IDD
Sbjct: 901  GGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVKEPRFKLLGQSRDSIATVLYIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYT P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTAPNAKHGFGFVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCYL SPWLCEPEEIE V PFKIGDQVCVKRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYLPSPWLCEPEEIELVAPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLL+I+LPNRHGPWKVDPSDMEKVEKF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLLIDLPNRHGPWKVDPSDMEKVEKFKVGDWVRVKGSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFR KTFPCSVTDIEKVPPFEVGQEIHILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRRKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKTERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNN  +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEKIPP+KIGQYV FRAGLAKP
Sbjct: 1261 SNNNGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKIPPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDDT 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMY VGEWVKLKDD 
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYEVGEWVKLKDDA 1380

Query: 1381 DGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVKV 1440
            +GRKSLPAGS+GVVQGLSYHENEWDGSVLVGFCREPE+WVGHTS+LEKTDRF+IGQRVKV
Sbjct: 1381 EGRKSLPAGSIGVVQGLSYHENEWDGSVLVGFCREPELWVGHTSELEKTDRFYIGQRVKV 1440

Query: 1441 KPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNIG 1500
            K SVP+PRFGWSGHSHA+IVSITAIDADGKIKVS+PSAQKPWTLDPSEVD VEEE+LNIG
Sbjct: 1441 KTSVPSPRFGWSGHSHANIVSITAIDADGKIKVSSPSAQKPWTLDPSEVDLVEEEKLNIG 1500

Query: 1501 DWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPFR 1560
            DWVKVKPSIVMPV+HWG+V+R+SVG++HKM+ GELWVAFCF+EQLW+CKDSEVEKVRPFR
Sbjct: 1501 DWVKVKPSIVMPVHHWGEVSRRSVGIIHKMDGGELWVAFCFMEQLWMCKDSEVEKVRPFR 1560

Query: 1561 VGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVLD 1620
            VGDAVRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVLD
Sbjct: 1561 VGDAVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVLD 1620

BLAST of MS010158 vs. ExPASy TrEMBL
Match: A0A6J1K4G2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111490691 PE=4 SV=1)

HSP 1 Score: 3107.4 bits (8055), Expect = 0.0e+00
Identity = 1489/1623 (91.74%), Postives = 1541/1623 (94.95%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICRTHYDEDSR PLLLQCGHTFC+HCLSQII PTPPKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRTHYDEDSRVPLLLQCGHTFCKHCLSQIIVPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVS  GNSVLSLPKNFAILPMISP SVSHSAEVSDSDDDDAG DEDG   SDRGRR+ G 
Sbjct: 61   HVSAVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDAGGDEDGVGDSDRGRRSYGF 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
             GCG GFGDHELKLVRKID  KR E+ELWFGWLRSR GGCRHRVV RRVRMGNVGDLDWV
Sbjct: 121  DGCGAGFGDHELKLVRKIDGEKRVEVELWFGWLRSRVGGCRHRVVVRRVRMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRASMWCRNVCSFLGVLKVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTVP-EQE 300
            GADIARAVVELHAADVLCM+LKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG  P E E
Sbjct: 241  GADIARAVVELHAADVLCMDLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPQEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSDAWSFGCALVEMCTGS
Sbjct: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDAWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGL+AEEIYRAVVKE RLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLTAEEIYRAVVKEGRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            IFL+HLQGI RSPT  ++E     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  IFLQHLQGIHRSPTKPSSEVVSSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNNSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAEC+RALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECLRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGC+SMGFLNSKNLTPLHMCITTLNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCKSMGFLNSKNLTPLHMCITTLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             WAELA PEEISEAIDIPS+TGTALCMAAALKKDRE EGRELVR+LLK+KA+PAAQDPQQ
Sbjct: 661  RWAELALPEEISEAIDIPSSTGTALCMAAALKKDRETEGRELVRILLKSKANPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIILDAGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDIELVKIILDAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AA  +RNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAATSIRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL EKGIHLSPT FQVGDWVKF+ CV NPAYGW+GAGPRSVGFVQGS SS
Sbjct: 841  REWIFEELMDALAEKGIHLSPTIFQVGDWVKFKSCVTNPAYGWQGAGPRSVGFVQGSHSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
             GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSI TVL IDD
Sbjct: 901  GGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSRDSIATVLYIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYTV  AKHGFG+VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTVSNAKHGFGSVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCYL SPWLCEPEEIE V PFKIGDQVC+KRSISEPRFPWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYLPSPWLCEPEEIELVAPFKIGDQVCIKRSISEPRFPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KVSDIESNGLL+I+LPNRHGPWKVDPSDMEKVEKF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVSDIESNGLLLIDLPNRHGPWKVDPSDMEKVEKFKVGDWVRVKGSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFR KTFPCSVTDIEKVPPFEVGQEIHILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRRKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK ERIDMDGTLNVRVSGKK LWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE
Sbjct: 1201 TPASSGKTERIDMDGTLNVRVSGKKKLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNN  +E IAVVYSIQDYSYLELACCFHEGK  VHCTEVEKIPP+KIGQYV FRAGLAKP
Sbjct: 1261 SNNNGKENIAVVYSIQDYSYLELACCFHEGKLFVHCTEVEKIPPIKIGQYVHFRAGLAKP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDDT 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMY VGEWVKLKDDT
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYEVGEWVKLKDDT 1380

Query: 1381 DGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVKV 1440
            +GRKSLPAGS+GVVQG SYHENEWDGSVLVGFCREPE+WVGHTS+LEKTDRF++GQRVKV
Sbjct: 1381 EGRKSLPAGSIGVVQGSSYHENEWDGSVLVGFCREPELWVGHTSELEKTDRFYVGQRVKV 1440

Query: 1441 KPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNIG 1500
            K SV NPRF WSGHSHA IVSITAIDADGKIKVS+PSAQKPWTLDPSEVD VEEE+LNIG
Sbjct: 1441 KTSVSNPRFRWSGHSHADIVSITAIDADGKIKVSSPSAQKPWTLDPSEVDLVEEEKLNIG 1500

Query: 1501 DWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPFR 1560
            DWVKVKPSIVMPV+HWG+V+RQSVG++HKM+ GELWVAFCF+EQLW+CKDSEVEKVRPFR
Sbjct: 1501 DWVKVKPSIVMPVHHWGEVSRQSVGIIHKMDGGELWVAFCFMEQLWMCKDSEVEKVRPFR 1560

Query: 1561 VGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVLD 1620
            VGDAVRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVLD
Sbjct: 1561 VGDAVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVLD 1620

BLAST of MS010158 vs. ExPASy TrEMBL
Match: A0A0A0KEJ0 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G430710 PE=4 SV=1)

HSP 1 Score: 3104.3 bits (8047), Expect = 0.0e+00
Identity = 1482/1624 (91.26%), Postives = 1548/1624 (95.32%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWK+VGIPSCSICR H+DEDSR+PLLLQCGHTFC+HCLSQIIAPT PKPSL CPKCR
Sbjct: 1    MPKKWKQVGIPSCSICRIHFDEDSRSPLLLQCGHTFCKHCLSQIIAPTSPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGC 120
            HVST GNSVLSLPKNFAILPMISP SVSHSAEV+DSDDDDAGADEDGAD+SDRGRR+SGC
Sbjct: 61   HVSTIGNSVLSLPKNFAILPMISPASVSHSAEVTDSDDDDAGADEDGADESDRGRRSSGC 120

Query: 121  HGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLDWV 180
            HGCG GFGDHELKLVRKID GKREE+ELWF WLRSR GGCRHRVV RRV+MGNVGDLDWV
Sbjct: 121  HGCGEGFGDHELKLVRKIDGGKREEMELWFAWLRSRVGGCRHRVVVRRVKMGNVGDLDWV 180

Query: 181  EKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQILRF 240
            EKQLEKLRRAS+WCRNVCSFLGV+KVED+LCIVMD FPGSVQSEMQRS  RLTLEQILRF
Sbjct: 181  EKQLEKLRRASIWCRNVCSFLGVMKVEDYLCIVMDWFPGSVQSEMQRSGGRLTLEQILRF 240

Query: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTV-PEQE 300
            GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG   PE E
Sbjct: 241  GADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPPEHE 300

Query: 301  SSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCTGS 360
            SS+QHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSD WSFGCALVEMCTGS
Sbjct: 301  SSRQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDVWSFGCALVEMCTGS 360

Query: 361  TPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420
            TPWAGLS EEIYR+VVKE +LPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA
Sbjct: 361  TPWAGLSTEEIYRSVVKEGKLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAMLA 420

Query: 421  IFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480
            +FLRHLQGI R PT  T E      IDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR
Sbjct: 421  VFLRHLQGIHRPPTRPTAEVASSPRIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNGVR 480

Query: 481  DLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDENG 540
            DLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAIL+YSDADIDSPDENG
Sbjct: 481  DLLSKSASGNNSSSVISLLEAHNSEGQTALHLACRRGSPELVDAILDYSDADIDSPDENG 540

Query: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAGAD 600
            NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AGAD
Sbjct: 541  NPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAGAD 600

Query: 601  PNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDVVK 660
            PNAVDDNGESVLHVAIAKKFT CAIVIMEHGGC+SMGFLNSKNLTPLHMCIT+LNVDVVK
Sbjct: 601  PNAVDDNGESVLHVAIAKKFTPCAIVIMEHGGCKSMGFLNSKNLTPLHMCITSLNVDVVK 660

Query: 661  MWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720
             W ELASPEEISEAIDIPS+TGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ
Sbjct: 661  RWVELASPEEISEAIDIPSSTGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDPQQ 720

Query: 721  CRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGANC 780
            CRTVLHTAAMAND ELVKIIL+AGVDVNI N+HNTIPLHLALARGAKPCVQLLLSAGANC
Sbjct: 721  CRTVLHTAAMANDVELVKIILNAGVDVNITNLHNTIPLHLALARGAKPCVQLLLSAGANC 780

Query: 781  NLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEALP 840
            NLQDDDGDNAFH+AADAAK+IRECL+CILLILKYP AAIGVRNH    GKTFCDLLEALP
Sbjct: 781  NLQDDDGDNAFHLAADAAKYIRECLDCILLILKYPGAAIGVRNHS---GKTFCDLLEALP 840

Query: 841  REWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQSS 900
            REWIFEELMDAL+EKGIHLSPT FQVGDWVKF+RCV NPAYGW+GAGPRSVGFVQGSQSS
Sbjct: 841  REWIFEELMDALEEKGIHLSPTIFQVGDWVKFKRCVTNPAYGWQGAGPRSVGFVQGSQSS 900

Query: 901  DGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCIDD 960
            DGLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKL  Q+RDSIGTVLCIDD
Sbjct: 901  DGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLFEQSRDSIGTVLCIDD 960

Query: 961  EEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGVVY 1020
            EEGIIRIGFTGASRG+QADPADFQRLQEFKVGDW+RVRYT+P AKHGFG VTPGSIGVVY
Sbjct: 961  EEGIIRIGFTGASRGFQADPADFQRLQEFKVGDWIRVRYTLPAAKHGFGDVTPGSIGVVY 1020

Query: 1021 GIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNSVG 1080
            GIRPDSSLLIEFCY+ SPWLCEPEEIEPVVPFKIGDQVCVKRSISEPR+PWDGETHNSVG
Sbjct: 1021 GIRPDSSLLIEFCYVQSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRYPWDGETHNSVG 1080

Query: 1081 KVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVPRN 1140
            KV DIESNGLLII+LPNRHGPWKVDPSDMEKV+KF+VGDWVRVK SVPSPKYGW+DVPR+
Sbjct: 1081 KVCDIESNGLLIIDLPNRHGPWKVDPSDMEKVDKFKVGDWVRVKTSVPSPKYGWDDVPRS 1140

Query: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWLDE 1200
            SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQE+HILPSVTQP LGW DE
Sbjct: 1141 SIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEVHILPSVTQPLLGWSDE 1200

Query: 1201 TPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSNYE 1260
            TPASSGK+ERIDMDGTLNVRVSG+K LWRVAPGDAEKLSGLAVGDWVR+KQCLG +SNYE
Sbjct: 1201 TPASSGKLERIDMDGTLNVRVSGRKKLWRVAPGDAEKLSGLAVGDWVRIKQCLGARSNYE 1260

Query: 1261 SNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLAKP 1320
            SNNT +E IAVVYSIQDYSY++LA CF EGKFPVHCTEVEKIPP+KIGQYV FRAGL  P
Sbjct: 1261 SNNTGKENIAVVYSIQDYSYVDLASCFREGKFPVHCTEVEKIPPIKIGQYVHFRAGLIIP 1320

Query: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKDD- 1380
            RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLK+D 
Sbjct: 1321 RWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKEDY 1380

Query: 1381 TDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRVK 1440
            TDGRKSLPAGS+GVVQGLSY ENEWDGSVLVGFCREPE+WVGHTSKLEKT+RF+IGQ VK
Sbjct: 1381 TDGRKSLPAGSIGVVQGLSYQENEWDGSVLVGFCREPELWVGHTSKLEKTERFYIGQHVK 1440

Query: 1441 VKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLNI 1500
            VKPS+PNPRFGWSGHSHASIVSITAIDADGKIKVS+ S QK W LDPSEV  VEEEQLNI
Sbjct: 1441 VKPSIPNPRFGWSGHSHASIVSITAIDADGKIKVSSSSPQKLWILDPSEVVMVEEEQLNI 1500

Query: 1501 GDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRPF 1560
            GDWVK+KPSIVMP YHWGDVTRQSVGV+HKMEDGELWVAFCF+EQLW+CKDSE+EKVRPF
Sbjct: 1501 GDWVKIKPSIVMPAYHWGDVTRQSVGVIHKMEDGELWVAFCFMEQLWMCKDSEMEKVRPF 1560

Query: 1561 RVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLVL 1620
            RVGD VRFREGLKIPRWGWGMETHAS+GQVVGVDAN KVRVRFRWREGRPWIGDPADLVL
Sbjct: 1561 RVGDTVRFREGLKIPRWGWGMETHASKGQVVGVDANGKVRVRFRWREGRPWIGDPADLVL 1620

BLAST of MS010158 vs. ExPASy TrEMBL
Match: A0A6J1FX97 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111448359 PE=4 SV=1)

HSP 1 Score: 3088.1 bits (8005), Expect = 0.0e+00
Identity = 1478/1625 (90.95%), Postives = 1543/1625 (94.95%), Query Frame = 0

Query: 1    MPKKWKRVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCR 60
            MPKKWKRVGIPSCSICR  YDEDSRAPLLLQCGHTFC+HCLSQIIAPTPPKPSL CPKCR
Sbjct: 1    MPKKWKRVGIPSCSICRIQYDEDSRAPLLLQCGHTFCKHCLSQIIAPTPPKPSLTCPKCR 60

Query: 61   HVSTAGNSVLSLPKNFAILPMISPGSVSHSAEVSDS--DDDDAGADEDGADQSDRGRRTS 120
            HVST GNSVLSLPKNFAILPMISP SVSHSAEVSDS  DDDDAGADE+G D SDRGRR+S
Sbjct: 61   HVSTVGNSVLSLPKNFAILPMISPASVSHSAEVSDSDDDDDDAGADEEGGDGSDRGRRSS 120

Query: 121  GCHGCGVGFGDHELKLVRKIDEGKREELELWFGWLRSRGGGCRHRVVARRVRMGNVGDLD 180
            GCHGCG GFGDHELKLVRKID GKREE+ELW GWLRSR GGCRHRV+ RRVR+GNVGDLD
Sbjct: 121  GCHGCGAGFGDHELKLVRKIDGGKREEMELWSGWLRSRVGGCRHRVMVRRVRIGNVGDLD 180

Query: 181  WVEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RLTLEQIL 240
            WVEKQLEKLRRASMWCRNVCSFLGVLKVE +LCIVM+ FPGSVQSEMQRS  RLTLEQIL
Sbjct: 181  WVEKQLEKLRRASMWCRNVCSFLGVLKVEGYLCIVMEGFPGSVQSEMQRSGGRLTLEQIL 240

Query: 241  RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGTV-PE 300
            RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAG   PE
Sbjct: 241  RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKPCHRAGIFPPE 300

Query: 301  QESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCALVEMCT 360
             ESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGIS+QSD WSFGCALVEMCT
Sbjct: 301  HESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISTQSDVWSFGCALVEMCT 360

Query: 361  GSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHAM 420
            GSTPWAGLSAEEIYRAVVK+ RLPPQYASIVGVGIP ELWKMIGECLQYKPLKRPTFHAM
Sbjct: 361  GSTPWAGLSAEEIYRAVVKDGRLPPQYASIVGVGIPGELWKMIGECLQYKPLKRPTFHAM 420

Query: 421  LAIFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNG 480
            LAIFLRHLQGI RSPT  T +     HIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNG
Sbjct: 421  LAIFLRHLQGIHRSPTRPTAKVASSPHIDRLEQSPTSVLDILQVKSNHLHQLVSEGDVNG 480

Query: 481  VRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDE 540
            VRDLLSKSASGNN SSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDE
Sbjct: 481  VRDLLSKSASGNNSSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDADIDSPDE 540

Query: 541  NGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLRAG 600
            NGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELL+AG
Sbjct: 541  NGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCMRELLQAG 600

Query: 601  ADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCITTLNVDV 660
            ADPNAVDDNGESVLHVAI+KKFTHCA+VIMEHGGC+SMGFLNS NLTPLH+CITTLNVDV
Sbjct: 601  ADPNAVDDNGESVLHVAISKKFTHCAMVIMEHGGCKSMGFLNSANLTPLHLCITTLNVDV 660

Query: 661  VKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQDP 720
            VK WAELA PEEISEAIDIPS+ GTALCMAAALKKDREIEGRELVRVLLKAKADPAAQ+P
Sbjct: 661  VKRWAELALPEEISEAIDIPSSAGTALCMAAALKKDREIEGRELVRVLLKAKADPAAQEP 720

Query: 721  QQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQLLLSAGA 780
            QQCRTVLHTAAMAND ELVKIIL+AGVDVNIRN+HNTIPLHLALARGAKPCVQLLLSAGA
Sbjct: 721  QQCRTVLHTAAMANDIELVKIILEAGVDVNIRNMHNTIPLHLALARGAKPCVQLLLSAGA 780

Query: 781  NCNLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKTFCDLLEA 840
            NCNLQDDDGDNAFHIAADAAKFIRECL+CILLILKYP AAIGVR H    GKTFCDLLEA
Sbjct: 781  NCNLQDDDGDNAFHIAADAAKFIRECLDCILLILKYPGAAIGVRTHS---GKTFCDLLEA 840

Query: 841  LPREWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSVGFVQGSQ 900
            LPREWIFEELMDAL EKGI LSPT FQVGDWVKF+RCVANPAYGW+GAGPRSVGFVQGSQ
Sbjct: 841  LPREWIFEELMDALAEKGIRLSPTIFQVGDWVKFKRCVANPAYGWQGAGPRSVGFVQGSQ 900

Query: 901  SSDGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDSIGTVLCI 960
            SS GLSVSFCSGVAHVLADEIIKVIP+DRGQLVQL+PDV EPRFKLLGQ+RDSIGTVLCI
Sbjct: 901  SSGGLSVSFCSGVAHVLADEIIKVIPMDRGQLVQLKPDVREPRFKLLGQSRDSIGTVLCI 960

Query: 961  DDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAVTPGSIGV 1020
            DDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDW+RVRYTVP A+HGF AV+PGSIGV
Sbjct: 961  DDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWIRVRYTVPAARHGFEAVSPGSIGV 1020

Query: 1021 VYGIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNS 1080
            VYGIRPDSSLLIEFCYL SPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNS
Sbjct: 1021 VYGIRPDSSLLIEFCYLPSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPWDGETHNS 1080

Query: 1081 VGKVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPKYGWEDVP 1140
            VGKV DIESNGLLII+LPNRHGPWKVDPSDMEKV+K++VGDWVRVKASVPSPKYGW+DVP
Sbjct: 1081 VGKVGDIESNGLLIIDLPNRHGPWKVDPSDMEKVDKYKVGDWVRVKASVPSPKYGWDDVP 1140

Query: 1141 RNSIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVTQPSLGWL 1200
            R+SIGIIF+LEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQE+HILPSVTQP LGW 
Sbjct: 1141 RSSIGIIFALEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEVHILPSVTQPLLGWS 1200

Query: 1201 DETPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQCLGTKSN 1260
            DETPASSGK+ER+DMDGTLNVRV GKK LWR+APGDAEKLSGL VGDWVRMKQCLGTKSN
Sbjct: 1201 DETPASSGKLERVDMDGTLNVRVFGKKKLWRIAPGDAEKLSGLEVGDWVRMKQCLGTKSN 1260

Query: 1261 YESNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYVRFRAGLA 1320
            YESN T +E IAVVYSIQDYS+LELACCF EGK  +H TEVEK+ P+KIGQYV FRAGLA
Sbjct: 1261 YESNYTGKENIAVVYSIQDYSHLELACCFREGKSFIHYTEVEKVSPIKIGQYVHFRAGLA 1320

Query: 1321 KPRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVGEWVKLKD 1380
            KPRWGWRGANPNSRGVVTAVNANGEIRVSLFGL+GWWRGDP DFEVEQMYAVG+WVKLKD
Sbjct: 1321 KPRWGWRGANPNSRGVVTAVNANGEIRVSLFGLAGWWRGDPTDFEVEQMYAVGDWVKLKD 1380

Query: 1381 DTDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDRFHIGQRV 1440
            DTDGRKSL AGS+GVVQGLSYHENEWDGSVLVGFC EPE+WVGHTSKLEK++RF IGQRV
Sbjct: 1381 DTDGRKSLSAGSIGVVQGLSYHENEWDGSVLVGFCGEPELWVGHTSKLEKSERFCIGQRV 1440

Query: 1441 KVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDPVEEEQLN 1500
            KVK  +PNPRFGWSGHSH S+VSITAIDADGKI+ S+P+AQKPWTLDPSEV  VEEEQLN
Sbjct: 1441 KVKSLIPNPRFGWSGHSHVSVVSITAIDADGKIRASSPAAQKPWTLDPSEVVLVEEEQLN 1500

Query: 1501 IGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDSEVEKVRP 1560
            IGDWVKVK  IVMPVYHWGDV+RQSVGV+HKMEDGELWVAFCF+E+LW+CKDSE+EKVRP
Sbjct: 1501 IGDWVKVKTLIVMPVYHWGDVSRQSVGVIHKMEDGELWVAFCFMERLWMCKDSEMEKVRP 1560

Query: 1561 FRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWIGDPADLV 1620
            FRVGDAVRFREGLKIPRWGWGMETHAS+G+VVGVDAN KVRVRFRWREGRPWIGDPADLV
Sbjct: 1561 FRVGDAVRFREGLKIPRWGWGMETHASKGKVVGVDANGKVRVRFRWREGRPWIGDPADLV 1620

BLAST of MS010158 vs. TAIR 10
Match: AT5G13530.1 (protein kinases;ubiquitin-protein ligases )

HSP 1 Score: 2131.3 bits (5521), Expect = 0.0e+00
Identity = 1006/1632 (61.64%), Postives = 1275/1632 (78.12%), Query Frame = 0

Query: 7    RVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCRHVSTAG 66
            RV +P CS+C T Y+ED R PLLLQCGH FC+ CLS++ + T    +L CP+CRHVS  G
Sbjct: 4    RVKVPCCSVCHTRYNEDERVPLLLQCGHGFCKDCLSKMFS-TSSDTTLTCPRCRHVSVVG 63

Query: 67   NSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGCHG---- 126
            NSV  L KN+A+L +I   S   + +   +DD+D   +EDG+D+ D  R   G H     
Sbjct: 64   NSVQGLRKNYAMLALIHAASGGANFDCDYTDDEDDDDEEDGSDE-DGARAARGFHASSSI 123

Query: 127  ---CG--VGFGDH-ELKLVRKIDE----GKREELELWFGWLRSRGGGCRHRVVARRVRMG 186
               CG  +  G H E+KLVR+I E    G    +E+W   +   GG C+HRV  +++ + 
Sbjct: 124  NSLCGPVIEVGAHPEMKLVRQIGEESSSGGFGGVEMWDATVAGGGGRCKHRVAVKKMTLT 183

Query: 187  NVGDLDWVEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RL 246
               D++W++ QLE LRRASMWCRNVC+F GV+K++  LC++MDR  GSVQSEMQR+  RL
Sbjct: 184  EDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSVQSEMQRNEGRL 243

Query: 247  TLEQILRFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKP-CHR 306
            TLEQILR+GAD+AR V ELHAA V+CMN+KPSN LLDA+G+AVVSDYGL  ILKKP C +
Sbjct: 244  TLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYGLAPILKKPTCQK 303

Query: 307  AGTVPEQESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCA 366
              T PE +SSK     +C+ LSPHY +PEAW P+K+   LF +D  G+S +SDAWSFGC 
Sbjct: 304  --TRPEFDSSKVTLYTDCVTLSPHYTAPEAWGPVKK---LFWEDASGVSPESDAWSFGCT 363

Query: 367  LVEMCTGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKR 426
            LVEMCTGSTPW GLS EEI++AVVK  ++PPQY  IVGVGIPRELWKMIGECLQ+KP KR
Sbjct: 364  LVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGECLQFKPSKR 423

Query: 427  PTFHAMLAIFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSV-LDILQVKSNHLHQLV 486
            PTF+AMLA FLRHLQ IPRSP+              + Q+P +  + + Q   N+LH++V
Sbjct: 424  PTFNAMLATFLRHLQEIPRSPSASPDNGIAKICEVNIVQAPRATNIGVFQDNPNNLHRVV 483

Query: 487  SEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDA 546
             EGD  GVR++L+K+A+G   SSV SLLEA N++GQ+ALHLACRRGS ELV+AILEY +A
Sbjct: 484  LEGDFEGVRNILAKAAAGGGGSSVRSLLEAQNADGQSALHLACRRGSAELVEAILEYGEA 543

Query: 547  DIDSPDENGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCM 606
            ++D  D++G+PP+VFALA GS +CV  LI+K AN   RL EG G SVAHVC+Y+GQPDCM
Sbjct: 544  NVDIVDKDGDPPLVFALAAGSPQCVHVLIKKGANVRSRLREGSGPSVAHVCSYHGQPDCM 603

Query: 607  RELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCI 666
            RELL AGADPNAVDD GE+VLH A+AKK+T CAIVI+E+GG RSM   N+K LTPLHMC+
Sbjct: 604  RELLVAGADPNAVDDEGETVLHRAVAKKYTDCAIVILENGGSRSMTVSNAKCLTPLHMCV 663

Query: 667  TTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKA 726
             T NV V+K W E++SPEEIS+AI+IPS  GTALCMAA+++KD E EGRELV++LL A A
Sbjct: 664  ATWNVAVIKRWVEVSSPEEISQAINIPSPVGTALCMAASIRKDHEKEGRELVQILLAAGA 723

Query: 727  DPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQ 786
            DP AQD Q  RT LHTAAMAN+ ELV++ILDAGV+ NIRNVHNTIPLH+ALARGA  CV 
Sbjct: 724  DPTAQDAQHGRTALHTAAMANNVELVRVILDAGVNANIRNVHNTIPLHMALARGANSCVS 783

Query: 787  LLLSAGANCNLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKT 846
            LLL +G++CN+QDD+GDNAFHIAADAAK IRE L+ ++++L+ PDAA+ VRNH    GKT
Sbjct: 784  LLLESGSDCNIQDDEGDNAFHIAADAAKMIRENLDWLIVMLRSPDAAVDVRNHS---GKT 843

Query: 847  FCDLLEALPREWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSV 906
              D LEALPREWI E+LM+AL ++G+HLSPT ++VGDWVKF+R +  P +GW+GA P+SV
Sbjct: 844  VRDFLEALPREWISEDLMEALLKRGVHLSPTIYEVGDWVKFKRGITTPLHGWQGAKPKSV 903

Query: 907  GFVQGSQSSDGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDS 966
            GFVQ     + + ++FCSG A VLA+E++K+IPLDRGQ V+LR DV EPRF   GQ+RDS
Sbjct: 904  GFVQTILEKEDMIIAFCSGEARVLANEVVKLIPLDRGQHVRLRADVKEPRFGWRGQSRDS 963

Query: 967  IGTVLCIDDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAV 1026
            +GTVLC+ DE+GI+R+GF GASRGW+ADPA+ +R++EFKVGDWVR+R  + +AKHGFG+V
Sbjct: 964  VGTVLCV-DEDGILRVGFPGASRGWKADPAEMERVEEFKVGDWVRIRQNLTSAKHGFGSV 1023

Query: 1027 TPGSIGVVYGIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPW 1086
             PGS+G+VY +RPDSSLL+E  YL +PW CEPEE+EPV PF+IGD+VCVKRS++EPR+ W
Sbjct: 1024 VPGSMGIVYCVRPDSSLLVELSYLPNPWHCEPEEVEPVAPFRIGDRVCVKRSVAEPRYAW 1083

Query: 1087 DGETHNSVGKVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPK 1146
             GETH+SVGK+S+IE++GLLIIE+PNR  PW+ DPSDMEK++ F+VGDWVRVKASV SPK
Sbjct: 1084 GGETHHSVGKISEIENDGLLIIEIPNRPIPWQADPSDMEKIDDFKVGDWVRVKASVSSPK 1143

Query: 1147 YGWEDVPRNSIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVT 1206
            YGWED+ RNSIG++ SL+EDGDV +AFCFRSK F CSVTD+EKV PF VGQEIH+ PS+T
Sbjct: 1144 YGWEDITRNSIGVMHSLDEDGDVGIAFCFRSKPFSCSVTDVEKVTPFHVGQEIHMTPSIT 1203

Query: 1207 QPSLGWLDETPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQ 1266
            QP LGW +ETPA+ GK+ RIDMDGTL+ +V+G++ LWRV+PGDAE LSG  VGDWVR K 
Sbjct: 1204 QPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELLSGFEVGDWVRSKP 1263

Query: 1267 CLGTKSNYESNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYV 1326
             LG + +Y+ +N  RE IAVV+SIQ+  YLELACCF +G++  H T++EKIP LK+GQ+V
Sbjct: 1264 SLGNRPSYDWSNVGRESIAVVHSIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFV 1323

Query: 1327 RFRAGLAKPRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVG 1386
             F+ G+ +PRWGWR A P+SRG++T V+A+GE+RV+ FGL G WRGDPAD EVE M+ VG
Sbjct: 1324 HFQKGITEPRWGWRAAKPDSRGIITTVHADGEVRVAFFGLPGLWRGDPADLEVEPMFEVG 1383

Query: 1387 EWVKLKDDTDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDR 1446
            EWV+L++     KS+  GSVGVV G+ Y  +EWDG+  V FC E E W G TS LEK  +
Sbjct: 1384 EWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAKK 1443

Query: 1447 FHIGQRVKVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDP 1506
              +GQ+ +VK +V  PRFGWSGHSH S+ +I+AIDADGK+++ TP+  K W LDPSEV+ 
Sbjct: 1444 LVVGQKTRVKLAVKQPRFGWSGHSHGSVGTISAIDADGKLRIYTPAGSKTWMLDPSEVET 1503

Query: 1507 VEEEQLNIGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDS 1566
            +EEE+L IGDWV+VK SI  P Y WG+V   S GVVH+MEDG+L V+FCF+++LW+CK  
Sbjct: 1504 IEEEELKIGDWVRVKASITTPTYQWGEVNPSSTGVVHRMEDGDLCVSFCFLDRLWLCKAG 1563

Query: 1567 EVEKVRPFRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWI 1621
            E+E++RPFR+GD V+ ++GL  PRWGWGMETHAS+G VVGVDAN K+R++F WREGRPWI
Sbjct: 1564 ELERIRPFRIGDRVKIKDGLVTPRWGWGMETHASKGHVVGVDANGKLRIKFLWREGRPWI 1623

BLAST of MS010158 vs. TAIR 10
Match: AT5G13530.2 (protein kinases;ubiquitin-protein ligases )

HSP 1 Score: 2126.3 bits (5508), Expect = 0.0e+00
Identity = 1005/1632 (61.58%), Postives = 1275/1632 (78.12%), Query Frame = 0

Query: 7    RVGIPSCSICRTHYDEDSRAPLLLQCGHTFCRHCLSQIIAPTPPKPSLACPKCRHVSTAG 66
            RV +P CS+C T Y+ED R PLLLQCGH FC+ CLS++ + T    +L CP+CRHVS  G
Sbjct: 4    RVKVPCCSVCHTRYNEDERVPLLLQCGHGFCKDCLSKMFS-TSSDTTLTCPRCRHVSVVG 63

Query: 67   NSVLSLPKNFAILPMISPGSVSHSAEVSDSDDDDAGADEDGADQSDRGRRTSGCHG---- 126
            NSV  L KN+A+L +I   S   + +   +DD+D   +EDG+D+ D  R   G H     
Sbjct: 64   NSVQGLRKNYAMLALIHAASGGANFDCDYTDDEDDDDEEDGSDE-DGARAARGFHASSSI 123

Query: 127  ---CG--VGFGDH-ELKLVRKIDE----GKREELELWFGWLRSRGGGCRHRVVARRVRMG 186
               CG  +  G H E+KLVR+I E    G    +E+W   +   GG C+HRV  +++ + 
Sbjct: 124  NSLCGPVIEVGAHPEMKLVRQIGEESSSGGFGGVEMWDATVAGGGGRCKHRVAVKKMTLT 183

Query: 187  NVGDLDWVEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQRS--RL 246
               D++W++ QLE LRRASMWCRNVC+F GV+K++  LC++MDR  GSVQSEMQR+  RL
Sbjct: 184  EDMDVEWMQGQLESLRRASMWCRNVCTFHGVVKMDGSLCLLMDRCFGSVQSEMQRNEGRL 243

Query: 247  TLEQILRFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLPLILKKP-CHR 306
            TLEQILR+GAD+AR V ELHAA V+CMN+KPSN LLDA+G+AVVSDYGL  ILKKP C +
Sbjct: 244  TLEQILRYGADVARGVAELHAAGVICMNIKPSNLLLDASGNAVVSDYGLAPILKKPTCQK 303

Query: 307  AGTVPEQESSKQHWCLECLFLSPHYRSPEAWEPLKRPLHLFRDDGIGISSQSDAWSFGCA 366
              T PE +SSK     +C+ LSPHY +PEAW P+K+   LF +D  G+S +SDAWSFGC 
Sbjct: 304  --TRPEFDSSKVTLYTDCVTLSPHYTAPEAWGPVKK---LFWEDASGVSPESDAWSFGCT 363

Query: 367  LVEMCTGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKR 426
            LVEMCTGSTPW GLS EEI++AVVK  ++PPQY  IVGVGIPRELWKMIGECLQ+KP KR
Sbjct: 364  LVEMCTGSTPWDGLSREEIFQAVVKARKVPPQYERIVGVGIPRELWKMIGECLQFKPSKR 423

Query: 427  PTFHAMLAIFLRHLQGIPRSPTGRTTEAPCFHHIDRLEQSPTSV-LDILQVKSNHLHQLV 486
            PTF+AMLA FLRHLQ IPRSP+              + Q+P +  + + Q   N+LH++V
Sbjct: 424  PTFNAMLATFLRHLQEIPRSPSASPDNGIAKICEVNIVQAPRATNIGVFQDNPNNLHRVV 483

Query: 487  SEGDVNGVRDLLSKSASGNNRSSVISLLEAHNSEGQTALHLACRRGSPELVDAILEYSDA 546
             EGD  GVR++L+K+A+G   SSV SLLEA N++GQ+ALHLACRRGS ELV+AILEY +A
Sbjct: 484  LEGDFEGVRNILAKAAAGGGGSSVRSLLEAQNADGQSALHLACRRGSAELVEAILEYGEA 543

Query: 547  DIDSPDENGNPPIVFALAVGSAECVRALIRKSANGMFRLMEGFGRSVAHVCAYYGQPDCM 606
            ++D  D++G+PP+VFALA GS +CV  LI+K AN   RL EG G SVAHVC+Y+GQPDCM
Sbjct: 544  NVDIVDKDGDPPLVFALAAGSPQCVHVLIKKGANVRSRLREGSGPSVAHVCSYHGQPDCM 603

Query: 607  RELLRAGADPNAVDDNGESVLHVAIAKKFTHCAIVIMEHGGCRSMGFLNSKNLTPLHMCI 666
            RELL AGADPNAVDD GE+VLH A+AKK+T CAIVI+E+GG RSM   N+K LTPLHMC+
Sbjct: 604  RELLVAGADPNAVDDEGETVLHRAVAKKYTDCAIVILENGGSRSMTVSNAKCLTPLHMCV 663

Query: 667  TTLNVDVVKMWAELASPEEISEAIDIPSATGTALCMAAALKKDREIEGRELVRVLLKAKA 726
             T NV V+K W E++SPEEIS+AI+IPS  GTALCMAA+++KD E +GRELV++LL A A
Sbjct: 664  ATWNVAVIKRWVEVSSPEEISQAINIPSPVGTALCMAASIRKDHE-KGRELVQILLAAGA 723

Query: 727  DPAAQDPQQCRTVLHTAAMANDAELVKIILDAGVDVNIRNVHNTIPLHLALARGAKPCVQ 786
            DP AQD Q  RT LHTAAMAN+ ELV++ILDAGV+ NIRNVHNTIPLH+ALARGA  CV 
Sbjct: 724  DPTAQDAQHGRTALHTAAMANNVELVRVILDAGVNANIRNVHNTIPLHMALARGANSCVS 783

Query: 787  LLLSAGANCNLQDDDGDNAFHIAADAAKFIRECLECILLILKYPDAAIGVRNHRQVFGKT 846
            LLL +G++CN+QDD+GDNAFHIAADAAK IRE L+ ++++L+ PDAA+ VRNH    GKT
Sbjct: 784  LLLESGSDCNIQDDEGDNAFHIAADAAKMIRENLDWLIVMLRSPDAAVDVRNHS---GKT 843

Query: 847  FCDLLEALPREWIFEELMDALKEKGIHLSPTTFQVGDWVKFRRCVANPAYGWKGAGPRSV 906
              D LEALPREWI E+LM+AL ++G+HLSPT ++VGDWVKF+R +  P +GW+GA P+SV
Sbjct: 844  VRDFLEALPREWISEDLMEALLKRGVHLSPTIYEVGDWVKFKRGITTPLHGWQGAKPKSV 903

Query: 907  GFVQGSQSSDGLSVSFCSGVAHVLADEIIKVIPLDRGQLVQLRPDVTEPRFKLLGQARDS 966
            GFVQ     + + ++FCSG A VLA+E++K+IPLDRGQ V+LR DV EPRF   GQ+RDS
Sbjct: 904  GFVQTILEKEDMIIAFCSGEARVLANEVVKLIPLDRGQHVRLRADVKEPRFGWRGQSRDS 963

Query: 967  IGTVLCIDDEEGIIRIGFTGASRGWQADPADFQRLQEFKVGDWVRVRYTVPTAKHGFGAV 1026
            +GTVLC+ DE+GI+R+GF GASRGW+ADPA+ +R++EFKVGDWVR+R  + +AKHGFG+V
Sbjct: 964  VGTVLCV-DEDGILRVGFPGASRGWKADPAEMERVEEFKVGDWVRIRQNLTSAKHGFGSV 1023

Query: 1027 TPGSIGVVYGIRPDSSLLIEFCYLSSPWLCEPEEIEPVVPFKIGDQVCVKRSISEPRFPW 1086
             PGS+G+VY +RPDSSLL+E  YL +PW CEPEE+EPV PF+IGD+VCVKRS++EPR+ W
Sbjct: 1024 VPGSMGIVYCVRPDSSLLVELSYLPNPWHCEPEEVEPVAPFRIGDRVCVKRSVAEPRYAW 1083

Query: 1087 DGETHNSVGKVSDIESNGLLIIELPNRHGPWKVDPSDMEKVEKFEVGDWVRVKASVPSPK 1146
             GETH+SVGK+S+IE++GLLIIE+PNR  PW+ DPSDMEK++ F+VGDWVRVKASV SPK
Sbjct: 1084 GGETHHSVGKISEIENDGLLIIEIPNRPIPWQADPSDMEKIDDFKVGDWVRVKASVSSPK 1143

Query: 1147 YGWEDVPRNSIGIIFSLEEDGDVDVAFCFRSKTFPCSVTDIEKVPPFEVGQEIHILPSVT 1206
            YGWED+ RNSIG++ SL+EDGDV +AFCFRSK F CSVTD+EKV PF VGQEIH+ PS+T
Sbjct: 1144 YGWEDITRNSIGVMHSLDEDGDVGIAFCFRSKPFSCSVTDVEKVTPFHVGQEIHMTPSIT 1203

Query: 1207 QPSLGWLDETPASSGKIERIDMDGTLNVRVSGKKNLWRVAPGDAEKLSGLAVGDWVRMKQ 1266
            QP LGW +ETPA+ GK+ RIDMDGTL+ +V+G++ LWRV+PGDAE LSG  VGDWVR K 
Sbjct: 1204 QPRLGWSNETPATIGKVMRIDMDGTLSAQVTGRQTLWRVSPGDAELLSGFEVGDWVRSKP 1263

Query: 1267 CLGTKSNYESNNTVREKIAVVYSIQDYSYLELACCFHEGKFPVHCTEVEKIPPLKIGQYV 1326
             LG + +Y+ +N  RE IAVV+SIQ+  YLELACCF +G++  H T++EKIP LK+GQ+V
Sbjct: 1264 SLGNRPSYDWSNVGRESIAVVHSIQETGYLELACCFRKGRWSTHYTDLEKIPALKVGQFV 1323

Query: 1327 RFRAGLAKPRWGWRGANPNSRGVVTAVNANGEIRVSLFGLSGWWRGDPADFEVEQMYAVG 1386
             F+ G+ +PRWGWR A P+SRG++T V+A+GE+RV+ FGL G WRGDPAD EVE M+ VG
Sbjct: 1324 HFQKGITEPRWGWRAAKPDSRGIITTVHADGEVRVAFFGLPGLWRGDPADLEVEPMFEVG 1383

Query: 1387 EWVKLKDDTDGRKSLPAGSVGVVQGLSYHENEWDGSVLVGFCREPEMWVGHTSKLEKTDR 1446
            EWV+L++     KS+  GSVGVV G+ Y  +EWDG+  V FC E E W G TS LEK  +
Sbjct: 1384 EWVRLREGVSCWKSVGPGSVGVVHGVGYEGDEWDGTTSVSFCGEQERWAGPTSHLEKAKK 1443

Query: 1447 FHIGQRVKVKPSVPNPRFGWSGHSHASIVSITAIDADGKIKVSTPSAQKPWTLDPSEVDP 1506
              +GQ+ +VK +V  PRFGWSGHSH S+ +I+AIDADGK+++ TP+  K W LDPSEV+ 
Sbjct: 1444 LVVGQKTRVKLAVKQPRFGWSGHSHGSVGTISAIDADGKLRIYTPAGSKTWMLDPSEVET 1503

Query: 1507 VEEEQLNIGDWVKVKPSIVMPVYHWGDVTRQSVGVVHKMEDGELWVAFCFVEQLWICKDS 1566
            +EEE+L IGDWV+VK SI  P Y WG+V   S GVVH+MEDG+L V+FCF+++LW+CK  
Sbjct: 1504 IEEEELKIGDWVRVKASITTPTYQWGEVNPSSTGVVHRMEDGDLCVSFCFLDRLWLCKAG 1563

Query: 1567 EVEKVRPFRVGDAVRFREGLKIPRWGWGMETHASEGQVVGVDANAKVRVRFRWREGRPWI 1621
            E+E++RPFR+GD V+ ++GL  PRWGWGMETHAS+G VVGVDAN K+R++F WREGRPWI
Sbjct: 1564 ELERIRPFRIGDRVKIKDGLVTPRWGWGMETHASKGHVVGVDANGKLRIKFLWREGRPWI 1623

BLAST of MS010158 vs. TAIR 10
Match: AT4G32250.1 (Protein kinase superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-32
Identity = 84/250 (33.60%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 180 VEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQR---SRLTLEQIL 239
           V  + E L        NVC   GV  +   +C+VM  + GS+  +M R    +L+L  +L
Sbjct: 87  VVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSLGDKMARLKGGKLSLPDVL 146

Query: 240 RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLP-LILKKPCHRAGTVPE 299
           R+G D+A  ++ELH+   L +NLKPSNFLL  N  A++ D G+P L+L  P      +P 
Sbjct: 147 RYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVGIPYLLLSIP------LPS 206

Query: 300 QESSKQHWCLECLFLSPHYRSPEAWEP-LKRPLHLFRDDGIGISSQSDAWSFGCALVEMC 359
            + +++         +P+Y +PE W+P ++ P+          S ++D+W FGC++VEM 
Sbjct: 207 SDMTER-------LGTPNYMAPEQWQPDVRGPM----------SFETDSWGFGCSIVEML 266

Query: 360 TGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHA 419
           TG  PW+G SA+EIY  VV++     Q    +   IP  L  ++  C  Y    RP+   
Sbjct: 267 TGVQPWSGRSADEIYDLVVRK-----QEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTD 307

Query: 420 MLAIFLRHLQ 425
           +L + L+ LQ
Sbjct: 327 ILLV-LKSLQ 307

BLAST of MS010158 vs. TAIR 10
Match: AT4G32250.2 (Protein kinase superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-32
Identity = 84/250 (33.60%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 180 VEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQR---SRLTLEQIL 239
           V  + E L        NVC   GV  +   +C+VM  + GS+  +M R    +L+L  +L
Sbjct: 87  VVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSLGDKMARLKGGKLSLPDVL 146

Query: 240 RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLP-LILKKPCHRAGTVPE 299
           R+G D+A  ++ELH+   L +NLKPSNFLL  N  A++ D G+P L+L  P      +P 
Sbjct: 147 RYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVGIPYLLLSIP------LPS 206

Query: 300 QESSKQHWCLECLFLSPHYRSPEAWEP-LKRPLHLFRDDGIGISSQSDAWSFGCALVEMC 359
            + +++         +P+Y +PE W+P ++ P+          S ++D+W FGC++VEM 
Sbjct: 207 SDMTER-------LGTPNYMAPEQWQPDVRGPM----------SFETDSWGFGCSIVEML 266

Query: 360 TGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHA 419
           TG  PW+G SA+EIY  VV++     Q    +   IP  L  ++  C  Y    RP+   
Sbjct: 267 TGVQPWSGRSADEIYDLVVRK-----QEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTD 307

Query: 420 MLAIFLRHLQ 425
           +L + L+ LQ
Sbjct: 327 ILLV-LKSLQ 307

BLAST of MS010158 vs. TAIR 10
Match: AT4G32250.3 (Protein kinase superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 1.1e-32
Identity = 84/250 (33.60%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 180 VEKQLEKLRRASMWCRNVCSFLGVLKVEDHLCIVMDRFPGSVQSEMQR---SRLTLEQIL 239
           V  + E L        NVC   GV  +   +C+VM  + GS+  +M R    +L+L  +L
Sbjct: 87  VVDKFEDLFSKCQGLENVCLLRGVSSINGKICVVMKFYEGSLGDKMARLKGGKLSLPDVL 146

Query: 240 RFGADIARAVVELHAADVLCMNLKPSNFLLDANGHAVVSDYGLP-LILKKPCHRAGTVPE 299
           R+G D+A  ++ELH+   L +NLKPSNFLL  N  A++ D G+P L+L  P      +P 
Sbjct: 147 RYGVDLATGILELHSKGFLILNLKPSNFLLSDNDKAILGDVGIPYLLLSIP------LPS 206

Query: 300 QESSKQHWCLECLFLSPHYRSPEAWEP-LKRPLHLFRDDGIGISSQSDAWSFGCALVEMC 359
            + +++         +P+Y +PE W+P ++ P+          S ++D+W FGC++VEM 
Sbjct: 207 SDMTER-------LGTPNYMAPEQWQPDVRGPM----------SFETDSWGFGCSIVEML 266

Query: 360 TGSTPWAGLSAEEIYRAVVKESRLPPQYASIVGVGIPRELWKMIGECLQYKPLKRPTFHA 419
           TG  PW+G SA+EIY  VV++     Q    +   IP  L  ++  C  Y    RP+   
Sbjct: 267 TGVQPWSGRSADEIYDLVVRK-----QEKLSIPSSIPPPLENLLRGCFMYDLRSRPSMTD 307

Query: 420 MLAIFLRHLQ 425
           +L + L+ LQ
Sbjct: 327 ILLV-LKSLQ 307