MS010147 (gene) Bitter gourd (TR) v1

BLAST of MS010147 vs. NCBI nr
Match: XP_022153031.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Momordica charantia] >XP_022153032.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Momordica charantia] >XP_022153033.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Momordica charantia] >XP_022153034.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Momordica charantia] >XP_022153035.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Momordica charantia])

HSP 1 Score: 846.7 bits (2186), Expect = 9.5e-242
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 60

Query: 82  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 141
           DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 120

Query: 142 RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR 201
           RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR
Sbjct: 121 RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR 180

Query: 202 VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC 261
           VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC
Sbjct: 181 VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC 240

Query: 262 SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS 321
           SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS
Sbjct: 241 SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS 300

Query: 322 LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA 381
           LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA
Sbjct: 301 LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA 360

Query: 382 GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 441
           GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA
Sbjct: 361 GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 420

Query: 442 LNRVAADSSKM 453
           LNRVAADSSKM
Sbjct: 421 LNRVAADSSKM 431

BLAST of MS010147 vs. NCBI nr
Match: KAG7035896.1 (Hydroxymethylglutaryl-CoA lyase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 773.5 bits (1996), Expect = 1.0e-219
Identity = 393/453 (86.75%), Postives = 425/453 (93.82%), Query Frame = 0

Query: 1   WQKTYFVYFSNTVLVFAFASTMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGM 60
           WQ+TY VYFSN+VLV A   TMSS+EEPLGLDKLPSMSTIDRIQRFSSGACRPIG+D+GM
Sbjct: 30  WQRTYLVYFSNSVLVLASTFTMSSVEEPLGLDKLPSMSTIDRIQRFSSGACRPIGDDIGM 89

Query: 61  GICWIQGGRSSSSNSCNEYDEDARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFC 120
           GICWI+GGRSS SNSC EYDEDARETFPWRRHRKD SQNGSVN+RTMS+G K  LSG+FC
Sbjct: 90  GICWIEGGRSSLSNSCREYDEDARETFPWRRHRKDASQNGSVNKRTMSIGSKKFLSGDFC 149

Query: 121 DSHCSPGRDYQTNSSGDNVQHRT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVEL 180
           DSHCS G +YQT S  DN+Q+ T KF+ G+PKFVKIVEVGPRDGLQNEK+MVPTSVKVEL
Sbjct: 150 DSHCSSGPEYQTESR-DNIQNMTNKFWNGIPKFVKIVEVGPRDGLQNEKDMVPTSVKVEL 209

Query: 181 IHRLVSSGLTVIEATSFVSPSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAA 240
           IHRLVSSGL V+EATSFVSP+RVPQLSDA+EVME VR+LEG RLPVLTPN+KGF AAVAA
Sbjct: 210 IHRLVSSGLPVVEATSFVSPTRVPQLSDATEVMEGVRNLEGVRLPVLTPNMKGFRAAVAA 269

Query: 241 GAKEVAVFASASESFSKSNINCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGA 300
           GAKEVAVFASASESFSKSNINCSIEESL RYRAVTSAAK+LGIPVRGYVSCVVGCPTEGA
Sbjct: 270 GAKEVAVFASASESFSKSNINCSIEESLDRYRAVTSAAKELGIPVRGYVSCVVGCPTEGA 329

Query: 301 VPPSKVAYVAKELYEMGCFEISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYG 360
           +PP KVAYVAK+LY+MGCFEISLGDTIGV TPGTVV MLEAV+A VPV+KLAVHFHDTYG
Sbjct: 330 IPPYKVAYVAKQLYDMGCFEISLGDTIGVATPGTVVQMLEAVMAVVPVDKLAVHFHDTYG 389

Query: 361 QSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKV 420
           QSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDV+YMLHGLGIKTNVDLAKV
Sbjct: 390 QSLPNILVSLQMGISTIDSSVAGLGGCPYAKGASGNVATEDVLYMLHGLGIKTNVDLAKV 449

Query: 421 MSAGNFISNHLGRPSGSKTAVALNRVAADSSKM 453
           +SAGNFI+NHLGRPSGSKTAVAL+RVAAD+SK+
Sbjct: 450 ISAGNFINNHLGRPSGSKTAVALSRVAADASKI 481

BLAST of MS010147 vs. NCBI nr
Match: XP_022985653.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 390/440 (88.64%), Postives = 415/440 (94.32%), Query Frame = 0

Query: 14  LVFAFASTMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSS 73
           LVFA ASTMSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSSS
Sbjct: 14  LVFASASTMSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSS 73

Query: 74  NSCNEYDEDARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTN 133
           N+C EYDEDARETFPWRRHRKDTSQNGSVN+RTMS G +  +SGNFCDSHCSPG +Y+T 
Sbjct: 74  NTCKEYDEDARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTK 133

Query: 134 SSGDNVQHRT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIE 193
           SS +N+QH T KF   +PKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGL V+E
Sbjct: 134 SSSNNIQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVE 193

Query: 194 ATSFVSPSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASE 253
           ATSFVSP+RVPQLSDA+EVMEAVR+LEGARLPVLTPNLKGF AAV AGAKEVAVFASASE
Sbjct: 194 ATSFVSPTRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASE 253

Query: 254 SFSKSNINCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKEL 313
           SFSKSNINCSIEE L RYRAVTSAAK+L IPVRGYVSCVVGCPTEG++PPSKVAYVAKEL
Sbjct: 254 SFSKSNINCSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKEL 313

Query: 314 YEMGCFEISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMG 373
           YEMGCFEISLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQMG
Sbjct: 314 YEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMG 373

Query: 374 ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR 433
           ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR
Sbjct: 374 ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR 433

Query: 434 PSGSKTAVALNRVAADSSKM 453
           PSGSKT++A N++AAD+SKM
Sbjct: 434 PSGSKTSIAFNQLAADASKM 453

BLAST of MS010147 vs. NCBI nr
Match: XP_022944769.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 770.0 bits (1987), Expect = 1.1e-218
Identity = 391/441 (88.66%), Postives = 415/441 (94.10%), Query Frame = 0

Query: 13  VLVFAFASTMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSS 72
           VLVFA ASTMSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSS
Sbjct: 13  VLVFASASTMSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSS 72

Query: 73  SNSCNEYDEDARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQT 132
           SN+C EYDEDARETFPWRRHRKDTSQNGSVN+RT S G +  +SGNFCDSHCSPG +Y+T
Sbjct: 73  SNTCKEYDEDARETFPWRRHRKDTSQNGSVNRRTSSAGLEKIVSGNFCDSHCSPGHEYKT 132

Query: 133 NSSGDNVQHRT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVI 192
            SS +N+QH T KF   +PKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGL V+
Sbjct: 133 KSSSNNIQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVV 192

Query: 193 EATSFVSPSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASAS 252
           EATSFVSP+RVPQLSDA+EVMEAVR+LEG RLPVLTPNLKGF AAVAAGAKEVAVFASAS
Sbjct: 193 EATSFVSPTRVPQLSDAAEVMEAVRNLEGVRLPVLTPNLKGFGAAVAAGAKEVAVFASAS 252

Query: 253 ESFSKSNINCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKE 312
           ESFSKSNINCSIEE L RYRAVTSAAK L IPVRGYVSCVVGCPTEG++PPSKVAYVAKE
Sbjct: 253 ESFSKSNINCSIEECLDRYRAVTSAAKALAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKE 312

Query: 313 LYEMGCFEISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQM 372
           LYEMGCFEISLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQM
Sbjct: 313 LYEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQM 372

Query: 373 GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG 432
           GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG
Sbjct: 373 GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG 432

Query: 433 RPSGSKTAVALNRVAADSSKM 453
           RPSGSKT++A N++AAD+SKM
Sbjct: 433 RPSGSKTSIAFNQLAADASKM 453

BLAST of MS010147 vs. NCBI nr
Match: XP_023512797.1 (hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo] >XP_023512798.1 hydroxymethylglutaryl-CoA lyase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.1 bits (1969), Expect = 1.4e-216
Identity = 385/432 (89.12%), Postives = 410/432 (94.91%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSSSN+C EYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 82  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 141
           DARETFPWRRHRKDTSQNGSVN+RTMS G +  +SGNFCDSHCSPG +Y+T SS +N+QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSAGPEKLVSGNFCDSHCSPGHEYKTKSSSNNIQH 120

Query: 142 RT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPS 201
            T KF   +PKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGL V+EATSFVSP+
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 202 RVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNIN 261
           RVPQLSDA+EVMEAVR+LEGARLPVLTPNLKGF AAVAAGAKEVAVFASASE FSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVAAGAKEVAVFASASELFSKSNIN 240

Query: 262 CSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 321
           CSIEESL RYRAVTSAAK+L IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEESLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 322 SLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381
           SLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAV 441
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT++
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 442 ALNRVAADSSKM 453
           A N++AAD+SKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of MS010147 vs. ExPASy Swiss-Prot
Match: O81027 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=HMGCL PE=1 SV=2)

HSP 1 Score: 589.7 bits (1519), Expect = 2.7e-167
Identity = 308/435 (70.80%), Postives = 362/435 (83.22%), Query Frame = 0

Query: 21  TMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYD 80
           TMSSLEEPL  DKLPSMST+DRIQRFSSGACRP  +D+GMG  WI+G   ++SNSC + D
Sbjct: 35  TMSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDD 94

Query: 81  ED-ARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNV 140
           +  A+E+FPWRRH +  S+   + +     GR +++SG   +S     + Y T S+ +  
Sbjct: 95  KSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGT 154

Query: 141 QH-RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVS 200
            H   K   G+PKFVKIVEVGPRDGLQNEKN+VPTSVKVELI RLVSSGL V+EATSFVS
Sbjct: 155 SHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVS 214

Query: 201 PSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSN 260
           P  VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASESFS SN
Sbjct: 215 PKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSN 274

Query: 261 INCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCF 320
           INC+IEESL+RYR V +AAK+  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGCF
Sbjct: 275 INCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCF 334

Query: 321 EISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDS 380
           EISLGDTIG+GTPG+VVPMLEAV+A VP +KLAVHFHDTYGQ+L NILVSLQMGIS +DS
Sbjct: 335 EISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDS 394

Query: 381 SVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT 440
           S+AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FIS HLGRP+GSK 
Sbjct: 395 SIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKA 454

Query: 441 AVALN-RVAADSSKM 453
           AVALN R+ AD+SK+
Sbjct: 455 AVALNRRITADASKI 468

BLAST of MS010147 vs. ExPASy Swiss-Prot
Match: Q8JZS7 (3-hydroxy-3-methylglutaryl-CoA lyase, cytoplasmic OS=Mus musculus OX=10090 GN=Hmgcll1 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 3.0e-105
Identity = 189/294 (64.29%), Postives = 237/294 (80.61%), Query Frame = 0

Query: 148 GMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSRVPQLSD 207
           G+P++VKIVEVGPRDGLQNEK +VPT +K+ELI++L  +GL+VIE TSFVS   VPQ++D
Sbjct: 43  GLPEYVKIVEVGPRDGLQNEKVIVPTDIKIELINQLSQTGLSVIEVTSFVSSRWVPQMAD 102

Query: 208 ASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINCSIEESL 267
            +EVM  +R   G R PVLTPNL+GF+ AVAAGA E+AVF +ASESFSK NINCSIEES+
Sbjct: 103 HAEVMRGIRQYPGVRYPVLTPNLQGFQHAVAAGATEIAVFGAASESFSKKNINCSIEESM 162

Query: 268 VRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDTIG 327
            R++ V S+A+ + IPVRGYVSC +GCP EG++ P KV  V+K LY MGC+EISLGDTIG
Sbjct: 163 GRFQEVISSARHMDIPVRGYVSCALGCPYEGSITPQKVTEVSKRLYGMGCYEISLGDTIG 222

Query: 328 VGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCP 387
           VGTPG++  MLE+V+  +P   LAVH HDTYGQ+L NIL +LQMGI+ +DS+V+GLGGCP
Sbjct: 223 VGTPGSMKMMLESVMKEIPPGALAVHCHDTYGQALANILTALQMGINVVDSAVSGLGGCP 282

Query: 388 YAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 442
           YAKGASGNVATED++YML+G+G+ T VDL KVM AG FI   + + + SK A A
Sbjct: 283 YAKGASGNVATEDLIYMLNGMGLNTGVDLYKVMEAGEFICKAVNKTTNSKVAQA 336

BLAST of MS010147 vs. ExPASy Swiss-Prot
Match: P35915 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Gallus gallus OX=9031 GN=HMGCL PE=1 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 5.1e-105
Identity = 194/296 (65.54%), Postives = 236/296 (79.73%), Query Frame = 0

Query: 150 PKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSRVPQLSDAS 209
           P+ VK+VEVGPRDGLQNEK++VPT VK+ LI  L  +GL VIEATSFVSP  VPQ++D +
Sbjct: 3   PQRVKVVEVGPRDGLQNEKSVVPTPVKIRLIDMLSETGLPVIEATSFVSPRWVPQMADHA 62

Query: 210 EVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINCSIEESLVR 269
           EVM+ +  L G   PVLTPNLKGF+AAVAAGAKEV++F +ASE F+K NINCSIEESL R
Sbjct: 63  EVMQGINKLPGVSYPVLTPNLKGFQAAVAAGAKEVSIFGAASELFTKKNINCSIEESLER 122

Query: 270 YRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISLGDTIGVG 329
           +  V +AA+   IPVRGYVSCV+GCP EG +  +KVA V+K++Y MGC+EISLGD IG+G
Sbjct: 123 FSEVMNAARAASIPVRGYVSCVLGCPYEGNISAAKVAEVSKKMYSMGCYEISLGDRIGIG 182

Query: 330 TPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAGLGGCPYA 389
           TPG++  ML AV+  VPV  LAVH HDTYGQ+L NILV+LQMG+S +D+SVAGLGGCPYA
Sbjct: 183 TPGSMKEMLAAVMKEVPVGALAVHCHDTYGQALANILVALQMGVSVVDASVAGLGGCPYA 242

Query: 390 KGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVALNRV 446
           +GASGNVATED+VYML+GLGI T VDL K+M  G FI N L R + SK + A  R+
Sbjct: 243 QGASGNVATEDLVYMLNGLGIHTGVDLQKLMDTGTFICNALNRRTNSKVSQAACRL 298

BLAST of MS010147 vs. ExPASy Swiss-Prot
Match: Q29448 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Bos taurus OX=9913 GN=HMGCL PE=2 SV=2)

HSP 1 Score: 382.1 bits (980), Expect = 8.7e-105
Identity = 195/299 (65.22%), Postives = 232/299 (77.59%), Query Frame = 0

Query: 143 TKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSRV 202
           T   G  PK VKIVEVGPRDGLQNEKN+VPT VK++LI  L  +GL V+EATSFVSP  V
Sbjct: 23  TSSVGTFPKQVKIVEVGPRDGLQNEKNIVPTPVKIKLIDMLSEAGLPVVEATSFVSPKWV 82

Query: 203 PQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINCS 262
           PQ++D +EV++ ++   G   PVLTPN KGF+AAVAAGAKEVA+F +ASE F+K NINCS
Sbjct: 83  PQMADHAEVLKGIQKFPGVNYPVLTPNFKGFQAAVAAGAKEVAIFGAASELFTKKNINCS 142

Query: 263 IEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISL 322
           I+ESL R+  +  AA+  GI VRGYVSCV+GCP EG + P+KVA V K+LY MGC+EISL
Sbjct: 143 IDESLQRFDEILKAARAAGISVRGYVSCVLGCPYEGKISPAKVAEVTKKLYSMGCYEISL 202

Query: 323 GDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAG 382
           GDTIGVGTPG +  ML AVL  VPV  LAVH HDTYGQ+L N L +LQMG+S +DSSVAG
Sbjct: 203 GDTIGVGTPGAMKDMLSAVLQEVPVTALAVHCHDTYGQALANTLTALQMGVSVMDSSVAG 262

Query: 383 LGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 442
           LGGCPYA+GASGN+ATED+VYML GLGI T V+L K++ AG FI   L R + SK A A
Sbjct: 263 LGGCPYAQGASGNLATEDLVYMLAGLGIHTGVNLQKLLEAGAFICQALNRRTNSKVAQA 321

BLAST of MS010147 vs. ExPASy Swiss-Prot
Match: Q5R9E1 (Hydroxymethylglutaryl-CoA lyase, mitochondrial OS=Pongo abelii OX=9601 GN=HMGCL PE=2 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 8.7e-105
Identity = 194/299 (64.88%), Postives = 232/299 (77.59%), Query Frame = 0

Query: 143 TKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSRV 202
           T   G +PK VKIVEVGPRDGLQNEKN+V T VK++LI  L  +GL+VIE TSFVSP  V
Sbjct: 23  TSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWV 82

Query: 203 PQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINCS 262
           PQ+ D +EV++ ++   G   PVLTPNLKGFEAAVAAGAKEVA+F +ASE F+K NINCS
Sbjct: 83  PQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVAIFGAASELFTKKNINCS 142

Query: 263 IEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEISL 322
           IEES  R+ A+  AA+   I VRGYVSC +GCP EG + P+KVA V K+LY MGC+EISL
Sbjct: 143 IEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKLYSMGCYEISL 202

Query: 323 GDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVAG 382
           GDTIGVGTPG +  ML AV+  VP+  LAVH HDTYGQ+L N L++LQMG+S +DSSVAG
Sbjct: 203 GDTIGVGTPGIMKGMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAG 262

Query: 383 LGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 442
           LGGCPYA+GASGN+ATED+VYML GLGI T V+L K++ AGNFI   L R + SK A A
Sbjct: 263 LGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQA 321

BLAST of MS010147 vs. ExPASy TrEMBL
Match: A0A6J1DFM2 (3-hydroxy-3-methylglutarate-CoA lyase OS=Momordica charantia OX=3673 GN=LOC111020633 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.6e-242
Identity = 431/431 (100.00%), Postives = 431/431 (100.00%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 60

Query: 82  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 141
           DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 120

Query: 142 RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR 201
           RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR
Sbjct: 121 RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPSR 180

Query: 202 VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC 261
           VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC
Sbjct: 181 VPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNINC 240

Query: 262 SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS 321
           SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS
Sbjct: 241 SIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEIS 300

Query: 322 LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA 381
           LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA
Sbjct: 301 LGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSVA 360

Query: 382 GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 441
           GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA
Sbjct: 361 GLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAVA 420

Query: 442 LNRVAADSSKM 453
           LNRVAADSSKM
Sbjct: 421 LNRVAADSSKM 431

BLAST of MS010147 vs. ExPASy TrEMBL
Match: A0A6J1JE93 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.5e-219
Identity = 390/440 (88.64%), Postives = 415/440 (94.32%), Query Frame = 0

Query: 14  LVFAFASTMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSS 73
           LVFA ASTMSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSSS
Sbjct: 14  LVFASASTMSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSS 73

Query: 74  NSCNEYDEDARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTN 133
           N+C EYDEDARETFPWRRHRKDTSQNGSVN+RTMS G +  +SGNFCDSHCSPG +Y+T 
Sbjct: 74  NTCKEYDEDARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTK 133

Query: 134 SSGDNVQHRT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIE 193
           SS +N+QH T KF   +PKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGL V+E
Sbjct: 134 SSSNNIQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVE 193

Query: 194 ATSFVSPSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASE 253
           ATSFVSP+RVPQLSDA+EVMEAVR+LEGARLPVLTPNLKGF AAV AGAKEVAVFASASE
Sbjct: 194 ATSFVSPTRVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASE 253

Query: 254 SFSKSNINCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKEL 313
           SFSKSNINCSIEE L RYRAVTSAAK+L IPVRGYVSCVVGCPTEG++PPSKVAYVAKEL
Sbjct: 254 SFSKSNINCSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKEL 313

Query: 314 YEMGCFEISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMG 373
           YEMGCFEISLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQMG
Sbjct: 314 YEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMG 373

Query: 374 ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR 433
           ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR
Sbjct: 374 ISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGR 433

Query: 434 PSGSKTAVALNRVAADSSKM 453
           PSGSKT++A N++AAD+SKM
Sbjct: 434 PSGSKTSIAFNQLAADASKM 453

BLAST of MS010147 vs. ExPASy TrEMBL
Match: A0A6J1FZ09 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449124 PE=3 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.5e-219
Identity = 391/441 (88.66%), Postives = 415/441 (94.10%), Query Frame = 0

Query: 13  VLVFAFASTMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSS 72
           VLVFA ASTMSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSS
Sbjct: 13  VLVFASASTMSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSS 72

Query: 73  SNSCNEYDEDARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQT 132
           SN+C EYDEDARETFPWRRHRKDTSQNGSVN+RT S G +  +SGNFCDSHCSPG +Y+T
Sbjct: 73  SNTCKEYDEDARETFPWRRHRKDTSQNGSVNRRTSSAGLEKIVSGNFCDSHCSPGHEYKT 132

Query: 133 NSSGDNVQHRT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVI 192
            SS +N+QH T KF   +PKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGL V+
Sbjct: 133 KSSSNNIQHTTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVV 192

Query: 193 EATSFVSPSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASAS 252
           EATSFVSP+RVPQLSDA+EVMEAVR+LEG RLPVLTPNLKGF AAVAAGAKEVAVFASAS
Sbjct: 193 EATSFVSPTRVPQLSDAAEVMEAVRNLEGVRLPVLTPNLKGFGAAVAAGAKEVAVFASAS 252

Query: 253 ESFSKSNINCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKE 312
           ESFSKSNINCSIEE L RYRAVTSAAK L IPVRGYVSCVVGCPTEG++PPSKVAYVAKE
Sbjct: 253 ESFSKSNINCSIEECLDRYRAVTSAAKALAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKE 312

Query: 313 LYEMGCFEISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQM 372
           LYEMGCFEISLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQM
Sbjct: 313 LYEMGCFEISLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQM 372

Query: 373 GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG 432
           GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG
Sbjct: 373 GISTIDSSVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLG 432

Query: 433 RPSGSKTAVALNRVAADSSKM 453
           RPSGSKT++A N++AAD+SKM
Sbjct: 433 RPSGSKTSIAFNQLAADASKM 453

BLAST of MS010147 vs. ExPASy TrEMBL
Match: A0A6J1JBX8 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111483647 PE=3 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.4e-216
Identity = 383/432 (88.66%), Postives = 408/432 (94.44%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSSSN+C EYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 82  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 141
           DARETFPWRRHRKDTSQNGSVN+RTMS G +  +SGNFCDSHCSPG +Y+T SS +N+QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTMSTGPEKLVSGNFCDSHCSPGHEYRTKSSSNNIQH 120

Query: 142 RT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPS 201
            T KF   +PKFVKIVEVGPRDGLQNEK MVPT VKVELIHRLVSSGL V+EATSFVSP+
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTFVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 202 RVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNIN 261
           RVPQLSDA+EVMEAVR+LEGARLPVLTPNLKGF AAV AGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGARLPVLTPNLKGFRAAVVAGAKEVAVFASASESFSKSNIN 240

Query: 262 CSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 321
           CSIEE L RYRAVTSAAK+L IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKELAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 322 SLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381
           SLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAV 441
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT++
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 442 ALNRVAADSSKM 453
           A N++AAD+SKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of MS010147 vs. ExPASy TrEMBL
Match: A0A6J1FZ07 (3-hydroxy-3-methylglutarate-CoA lyase OS=Cucurbita moschata OX=3662 GN=LOC111449124 PE=3 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.2e-215
Identity = 383/432 (88.66%), Postives = 407/432 (94.21%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPLGLDKLPSMSTIDR+QRFSSGACRPIG+DMGMGICWIQGGRSSSSN+C EYDE
Sbjct: 1   MSSLEEPLGLDKLPSMSTIDRLQRFSSGACRPIGDDMGMGICWIQGGRSSSSNTCKEYDE 60

Query: 82  DARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQH 141
           DARETFPWRRHRKDTSQNGSVN+RT S G +  +SGNFCDSHCSPG +Y+T SS +N+QH
Sbjct: 61  DARETFPWRRHRKDTSQNGSVNRRTSSAGLEKIVSGNFCDSHCSPGHEYKTKSSSNNIQH 120

Query: 142 RT-KFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSPS 201
            T KF   +PKFVKIVEVGPRDGLQNEK MVPTSVKVELIHRLVSSGL V+EATSFVSP+
Sbjct: 121 TTNKFLKDIPKFVKIVEVGPRDGLQNEKGMVPTSVKVELIHRLVSSGLPVVEATSFVSPT 180

Query: 202 RVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNIN 261
           RVPQLSDA+EVMEAVR+LEG RLPVLTPNLKGF AAVAAGAKEVAVFASASESFSKSNIN
Sbjct: 181 RVPQLSDAAEVMEAVRNLEGVRLPVLTPNLKGFGAAVAAGAKEVAVFASASESFSKSNIN 240

Query: 262 CSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFEI 321
           CSIEE L RYRAVTSAAK L IPVRGYVSCVVGCPTEG++PPSKVAYVAKELYEMGCFEI
Sbjct: 241 CSIEECLDRYRAVTSAAKALAIPVRGYVSCVVGCPTEGSIPPSKVAYVAKELYEMGCFEI 300

Query: 322 SLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 381
           SLGDTIGV TPGTVVPMLEAV+A VPV+KLAVHFHDTYGQSLPNILVSLQMGISTIDSSV
Sbjct: 301 SLGDTIGVATPGTVVPMLEAVMAVVPVDKLAVHFHDTYGQSLPNILVSLQMGISTIDSSV 360

Query: 382 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTAV 441
           AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT++
Sbjct: 361 AGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTSI 420

Query: 442 ALNRVAADSSKM 453
           A N++AAD+SKM
Sbjct: 421 AFNQLAADASKM 432

BLAST of MS010147 vs. TAIR 10
Match: AT2G26800.2 (Aldolase superfamily protein )

HSP 1 Score: 589.7 bits (1519), Expect = 2.0e-168
Identity = 308/435 (70.80%), Postives = 362/435 (83.22%), Query Frame = 0

Query: 21  TMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYD 80
           TMSSLEEPL  DKLPSMST+DRIQRFSSGACRP  +D+GMG  WI+G   ++SNSC + D
Sbjct: 35  TMSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDD 94

Query: 81  ED-ARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNV 140
           +  A+E+FPWRRH +  S+   + +     GR +++SG   +S     + Y T S+ +  
Sbjct: 95  KSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGT 154

Query: 141 QH-RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVS 200
            H   K   G+PKFVKIVEVGPRDGLQNEKN+VPTSVKVELI RLVSSGL V+EATSFVS
Sbjct: 155 SHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVS 214

Query: 201 PSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSN 260
           P  VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASESFS SN
Sbjct: 215 PKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSN 274

Query: 261 INCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCF 320
           INC+IEESL+RYR V +AAK+  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGCF
Sbjct: 275 INCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCF 334

Query: 321 EISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDS 380
           EISLGDTIG+GTPG+VVPMLEAV+A VP +KLAVHFHDTYGQ+L NILVSLQMGIS +DS
Sbjct: 335 EISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDS 394

Query: 381 SVAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKT 440
           S+AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FIS HLGRP+GSK 
Sbjct: 395 SIAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKA 454

Query: 441 AVALN-RVAADSSKM 453
           AVALN R+ AD+SK+
Sbjct: 455 AVALNRRITADASKI 468

BLAST of MS010147 vs. TAIR 10
Match: AT2G26800.1 (Aldolase superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 7.4e-168
Identity = 307/434 (70.74%), Postives = 361/434 (83.18%), Query Frame = 0

Query: 22  MSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYDE 81
           MSSLEEPL  DKLPSMST+DRIQRFSSGACRP  +D+GMG  WI+G   ++SNSC + D+
Sbjct: 1   MSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDDK 60

Query: 82  D-ARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNVQ 141
             A+E+FPWRRH +  S+   + +     GR +++SG   +S     + Y T S+ +   
Sbjct: 61  SFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGTS 120

Query: 142 H-RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVSP 201
           H   K   G+PKFVKIVEVGPRDGLQNEKN+VPTSVKVELI RLVSSGL V+EATSFVSP
Sbjct: 121 HISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVSP 180

Query: 202 SRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSNI 261
             VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASESFS SNI
Sbjct: 181 KWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSNI 240

Query: 262 NCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCFE 321
           NC+IEESL+RYR V +AAK+  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGCFE
Sbjct: 241 NCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCFE 300

Query: 322 ISLGDTIGVGTPGTVVPMLEAVLAFVPVEKLAVHFHDTYGQSLPNILVSLQMGISTIDSS 381
           ISLGDTIG+GTPG+VVPMLEAV+A VP +KLAVHFHDTYGQ+L NILVSLQMGIS +DSS
Sbjct: 301 ISLGDTIGIGTPGSVVPMLEAVMAVVPADKLAVHFHDTYGQALANILVSLQMGISIVDSS 360

Query: 382 VAGLGGCPYAKGASGNVATEDVVYMLHGLGIKTNVDLAKVMSAGNFISNHLGRPSGSKTA 441
           +AGLGGCPYAKGASGNVATEDVVYML+GLG+ TNVDL K+++AG+FIS HLGRP+GSK A
Sbjct: 361 IAGLGGCPYAKGASGNVATEDVVYMLNGLGVHTNVDLGKLIAAGDFISKHLGRPNGSKAA 420

Query: 442 VALN-RVAADSSKM 453
           VALN R+ AD+SK+
Sbjct: 421 VALNRRITADASKI 433

BLAST of MS010147 vs. TAIR 10
Match: AT2G26800.3 (Aldolase superfamily protein )

HSP 1 Score: 403.3 bits (1035), Expect = 2.6e-112
Identity = 214/317 (67.51%), Postives = 252/317 (79.50%), Query Frame = 0

Query: 21  TMSSLEEPLGLDKLPSMSTIDRIQRFSSGACRPIGNDMGMGICWIQGGRSSSSNSCNEYD 80
           TMSSLEEPL  DKLPSMST+DRIQRFSSGACRP  +D+GMG  WI+G   ++SNSC + D
Sbjct: 35  TMSSLEEPLSFDKLPSMSTMDRIQRFSSGACRP-RDDVGMGHRWIEGRDCTTSNSCIDDD 94

Query: 81  ED-ARETFPWRRHRKDTSQNGSVNQRTMSLGRKNSLSGNFCDSHCSPGRDYQTNSSGDNV 140
           +  A+E+FPWRRH +  S+   + +     GR +++SG   +S     + Y T S+ +  
Sbjct: 95  KSFAKESFPWRRHTRKLSEGEHMFRNISFAGRTSTVSGTLRESKSFKEQKYSTFSNENGT 154

Query: 141 QH-RTKFFGGMPKFVKIVEVGPRDGLQNEKNMVPTSVKVELIHRLVSSGLTVIEATSFVS 200
            H   K   G+PKFVKIVEVGPRDGLQNEKN+VPTSVKVELI RLVSSGL V+EATSFVS
Sbjct: 155 SHISNKISKGIPKFVKIVEVGPRDGLQNEKNIVPTSVKVELIQRLVSSGLPVVEATSFVS 214

Query: 201 PSRVPQLSDASEVMEAVRDLEGARLPVLTPNLKGFEAAVAAGAKEVAVFASASESFSKSN 260
           P  VPQL+DA +VM+AV  L+GARLPVLTPNLKGF+AAV+AGAKEVA+FASASESFS SN
Sbjct: 215 PKWVPQLADAKDVMDAVNTLDGARLPVLTPNLKGFQAAVSAGAKEVAIFASASESFSLSN 274

Query: 261 INCSIEESLVRYRAVTSAAKKLGIPVRGYVSCVVGCPTEGAVPPSKVAYVAKELYEMGCF 320
           INC+IEESL+RYR V +AAK+  +PVRGYVSCVVGCP EG V PSKVAYV KELY+MGCF
Sbjct: 275 INCTIEESLLRYRVVATAAKEHSVPVRGYVSCVVGCPVEGPVLPSKVAYVVKELYDMGCF 334

Query: 321 EISLGDTIGVGTPGTVV 336
           EISLGDTIG+GTPG  V
Sbjct: 335 EISLGDTIGIGTPGNFV 350