MS010139 (gene) Bitter gourd (TR) v1

BLAST of MS010139 vs. NCBI nr
Match: XP_022152982.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 761.5 bits (1965), Expect = 3.3e-216
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA 60
           MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA
Sbjct: 1   MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA 60

Query: 61  EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY 120
           EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY
Sbjct: 61  EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY 120

Query: 121 GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL 180
           GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL
Sbjct: 121 GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL 180

Query: 181 MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK 240
           MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK
Sbjct: 181 MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK 240

Query: 241 LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG 300
           LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG
Sbjct: 241 LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG 300

Query: 301 WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP 360
           WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP
Sbjct: 301 WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP 360

Query: 361 PTDPKA 367
           PTDPKA
Sbjct: 361 PTDPKA 366

BLAST of MS010139 vs. NCBI nr
Match: XP_022152989.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 562.8 bits (1449), Expect = 2.2e-156
Identity = 268/360 (74.44%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVV 67
           +L+ LLA      I   SPP+P DK+++N WFS+NVKPLA R+AELDP LVAAE+ A VV
Sbjct: 12  RLLTLLATF--ALILAASPPVPADKSQVNKWFSDNVKPLADRKAELDPALVAAEEGATVV 71

Query: 68  KVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVP 127
           KV ADGSGDFKT+TEAINSVP GNT RVVIWIGGGVYKEKL ++R KPFVTLYGSP N+P
Sbjct: 72  KVRADGSGDFKTVTEAINSVPTGNTKRVVIWIGGGVYKEKLKIERTKPFVTLYGSPNNMP 131

Query: 128 NLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKAA 187
           NLTFDG A K+GTVYSATL VESDYFTAANL I+N+SPRPDGK  G QALAARLMG KAA
Sbjct: 132 NLTFDGDANKFGTVYSATLTVESDYFTAANLIIVNSSPRPDGKRKGAQALAARLMGTKAA 191

Query: 188 VYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITA 247
           VYNCKF GFQDTLCDD GLHL+KDC+IQGT+DFIFG+GTSLYLNS+LDV+GDG L VITA
Sbjct: 192 VYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNSQLDVVGDGGLAVITA 251

Query: 248 HSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRH 307
           HSREEESD SGYSFVH SI GTGG+NTYLGRAW  RS+T+F+YTT+ADI++PEGW+DM+H
Sbjct: 252 HSREEESDPSGYSFVHGSITGTGGKNTYLGRAWMARSRTVFAYTTMADIINPEGWNDMKH 311

Query: 308 HGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPTDPKA 367
            GFD+TV F E+KC+GPG++ +GRVKY K+L+D EVKP+++L F+QSDKWLLPPP +P+A
Sbjct: 312 PGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLSDVEVKPFVSLGFLQSDKWLLPPP-NPEA 368

BLAST of MS010139 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 562.4 bits (1448), Expect = 2.9e-156
Identity = 266/358 (74.30%), Postives = 307/358 (85.75%), Query Frame = 0

Query: 5   GGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNA 64
           G  +LV+ LAA     I+ VSPP+P +K++L  WFSENVKP A  +AELDP LVAAE+NA
Sbjct: 9   GLTRLVIFLAAF--AAIFAVSPPVPAEKSQLEGWFSENVKPFADCKAELDPALVAAEENA 68

Query: 65  KVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPA 124
            VVKV ADGSGDFKT+TEAINSVP GNT RVVIWIG GVYKEKL +DR KPFVTLYGSP 
Sbjct: 69  TVVKVRADGSGDFKTVTEAINSVPAGNTRRVVIWIGEGVYKEKLKIDRNKPFVTLYGSPN 128

Query: 125 NVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGD 184
           N+PNLTFDG A KYGTVYSATLIVESDYF AANL I N+SPRPDG + G QALAARLMG 
Sbjct: 129 NMPNLTFDGDAAKYGTVYSATLIVESDYFVAANLVIENSSPRPDGIRKGAQALAARLMGT 188

Query: 185 KAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKV 244
           KAA+YNCKF GFQDTLCDD GLHL+KDC+IQGT+DFIFG+GTSLYLNS LDV+GDG L V
Sbjct: 189 KAAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFIFGKGTSLYLNSRLDVVGDGGLAV 248

Query: 245 ITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHD 304
           ITAHSREEESD+SGYSF HCSI GTGG+NTYLGRAW PRS+ +F+YTT+ADI+H EGW+D
Sbjct: 249 ITAHSREEESDSSGYSFAHCSITGTGGKNTYLGRAWMPRSRVVFAYTTMADIIHAEGWND 308

Query: 305 MRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
           M H G+DATV F E+KC+GPGA+ +GRVKY K+L+D EVKP+++L ++QS+KWLLPPP
Sbjct: 309 MNHSGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDVEVKPFISLGYLQSEKWLLPPP 364

BLAST of MS010139 vs. NCBI nr
Match: XP_011657599.1 (putative pectinesterase 63 [Cucumis sativus])

HSP 1 Score: 537.3 bits (1383), Expect = 1.0e-148
Identity = 258/358 (72.07%), Postives = 300/358 (83.80%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGR-EAELDPKLVAAEKNAKV 67
           +LV  LA      I  VSPPIP +K++L  WF+ENVKP A R +AELDP L  AE+NA V
Sbjct: 12  RLVTFLATF--TAILAVSPPIPVEKSQLAGWFTENVKPFAVRNKAELDPALATAEENATV 71

Query: 68  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGS-PAN 127
           +KVM+DG+G+FKT+TEAI SVP  N  RVVIWIG GVYKEKL +DR KPFVTLYGS P N
Sbjct: 72  IKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLYGSDPKN 131

Query: 128 VPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGDK 187
           +P LTFDG A KYGTVYSATLIVE+DYFTAANL I N+SPRPDG + G QALAAR MG K
Sbjct: 132 MPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTK 191

Query: 188 AAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVI 247
           AA+YNCKF GFQDTLCDD GLH +KDC+IQGT+DFIFG+GTSLYLN++LDV GDG L VI
Sbjct: 192 AAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVI 251

Query: 248 TAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDM 307
           TAHSRE+E+DTSGYSFVHCSI GTGG+NTYLGRAW PRS+ +F+YTTIADI+HPEGW+DM
Sbjct: 252 TAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDM 311

Query: 308 RHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPT 363
           +H GFD TV F E+KC+GPGA  TGRV YGK+LTD EVKP+L L+++QS+KWLLPPP+
Sbjct: 312 KHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPS 367

BLAST of MS010139 vs. NCBI nr
Match: XP_016900732.1 (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 526.9 bits (1356), Expect = 1.4e-145
Identity = 248/355 (69.86%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVV 67
           +LV  LA      I  VS PIP +K+++++WFS+NVKPLA R+AELDP LVAAE+NA ++
Sbjct: 8   RLVTFLATF--TTILAVSTPIPVEKSQIDAWFSQNVKPLADRKAELDPALVAAEENATII 67

Query: 68  KVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVP 127
           KVM+DG+GDFKTITEAI SVP  N  RV+I IG G+YKEKL +DR KPFVTLYGSP  +P
Sbjct: 68  KVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYGSPKGMP 127

Query: 128 NLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGDKAA 187
            LTFDG A K+GTVYSATLIVE+DYFTAANL I N+SPRPDG + G QALAAR MG KAA
Sbjct: 128 KLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARFMGTKAA 187

Query: 188 VYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITA 247
           +YNCKF GFQDTLCDD GLH +KDC+IQGT+DFIFG+GTSLY+N++LDV GDG L VITA
Sbjct: 188 IYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDGGLAVITA 247

Query: 248 HSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRH 307
           HSRE+ESD SGYSFVHCSI GTGG+NT+LGRAW PRS+ +F+YTT+ADI+HPEGW+DM+H
Sbjct: 248 HSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPEGWNDMKH 307

Query: 308 HGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
            GFD TV F E+KC+GPG+  T RV YGK+L+D EV P+L+L ++QS+KWLLPPP
Sbjct: 308 AGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLPPP 360

BLAST of MS010139 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.4e-105
Identity = 184/356 (51.69%), Postives = 247/356 (69.38%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I L +L+  V P+       PIP  K ++  WF+ NV PLA R+  LDP LVAAE   ++
Sbjct: 8   ILLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQRKG-LDPALVAAEAAPRI 67

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V   G G+FKT+T+AI SVP GNT RV+I +  G YKEK+ +DR KPF+TL G P  +
Sbjct: 68  INVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAM 127

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P +T+DG A KYGTV SA+LI+ SDYF A N+ + NT+P PDGK  G QAL+ R+ G+ A
Sbjct: 128 PVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFA 187

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYGFQDT+CDD G H FKDCY++GT DFIFG GTS+YL ++L V+GDG ++VI 
Sbjct: 188 AFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG-IRVIA 247

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +   + SGYSFVHC + GTGG   YLGRAW    K +++YT +  +++P GW + +
Sbjct: 248 AHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 307

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
               D TVF+ E+KC+GPG+ +  RV + +++ DKE   +L+L +IQ  KWLLPPP
Sbjct: 308 TPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPP 359

BLAST of MS010139 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 5.4e-105
Identity = 186/357 (52.10%), Postives = 243/357 (68.07%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           + L+V  A+  P+ +     PIP D+ ++  WF  NVKP + R   LDP+L AAE + +V
Sbjct: 9   VALLVFFAS--PVVLADDITPIPADRAQIPQWFMANVKPFSQRRGTLDPELEAAEASRRV 68

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V  +G GDFKTI  AI S+P  N NRV+I +  G+Y EK+ VD  +P+VTL G P   
Sbjct: 69  IIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPGAE 128

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQ--QALAARLMGDK 186
            NLT+ G A KYGTV SATLIV +  F AANLNIINTSP P  KPG   QALA R+ GDK
Sbjct: 129 TNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMP--KPGTQGQALAMRINGDK 188

Query: 187 AAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVI 246
           AA YNC+FYGFQDTLCDD G H FK+CYI+GT DFIFGRG SLYL ++L  +GDG L+VI
Sbjct: 189 AAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDG-LRVI 248

Query: 247 TAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDM 306
            AH+R+  ++ +GYSFVHC + G  G   YLGRAW    K ++SYT ++ +++P GW + 
Sbjct: 249 AAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQEN 308

Query: 307 RHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
           R    D TVF+ E+ CTGPG+ +  RV + +++ +KE   +LTL +I+  KWLLPPP
Sbjct: 309 RVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

BLAST of MS010139 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 378.6 bits (971), Expect = 7.8e-104
Identity = 182/356 (51.12%), Postives = 247/356 (69.38%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I L +L+  V P+       PIP  K ++  WF+ +V PLA R+  LDP LVAAE   ++
Sbjct: 8   ILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQRKG-LDPALVAAEAAPRI 67

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V   G G+FKT+T+AI SVP GNT RV+I +  G Y+EK+ +DR KPF+TL G P  +
Sbjct: 68  INVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAM 127

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P +T+DG A KYGTV SA+LI+ SDYF A N+ + NT+P PDGK  G QAL+ R+ G+ A
Sbjct: 128 PVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFA 187

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYGFQDT+CDD G H FKDCY++GT DFIFG GTS+YL ++L V+GDG ++VI 
Sbjct: 188 AFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG-IRVIA 247

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +   + SGYSFVHC + GTGG   YLGRAW    K +++YT +  +++P GW + +
Sbjct: 248 AHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 307

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
               D TVF+ E+KC+GPG+ +  RV + +++ DKE   +L+L +IQ  KWLLPPP
Sbjct: 308 TPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPP 359

BLAST of MS010139 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 372.9 bits (956), Expect = 4.3e-102
Identity = 180/357 (50.42%), Postives = 240/357 (67.23%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I  ++L+    P+     + PIP +K ++  WF+ NVK                      
Sbjct: 9   IVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVK---------------------- 68

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
                +G G FKTITEAINSV  GNT RV+I IG GVYKEK+ +DR+KPF+TLYG P  +
Sbjct: 69  ----QNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAM 128

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P LTFDG A +YGTV SATLIV SDYF A N+ + N++P PDGK  G QAL+ R+ G+KA
Sbjct: 129 PVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKA 188

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYG+QDT+CDD G H FKDCYI+GT DFIFG G SLYL ++L+V+GDG ++VIT
Sbjct: 189 AFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDG-IRVIT 248

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +  ++ SGYSFVHC + GT G   YLGR+W    K +++YT ++ +++P GW + R
Sbjct: 249 AHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENR 308

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPT 363
             G D TVF+ E+KCTG G+ +  RVKY +++ D E K +++L +IQ   WLLPPP+
Sbjct: 309 EAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

BLAST of MS010139 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 1.1e-100
Identity = 174/352 (49.43%), Postives = 238/352 (67.61%), Query Frame = 0

Query: 12  LLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVVKVMA 71
           L+    P+ +   + PIP ++ ++  WF  NVKP + R+  LDP L AAE   +++ V  
Sbjct: 12  LVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGTLDPALEAAEAARQIITVNQ 71

Query: 72  DGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVPNLTF 131
            G  +FKT+ EAI S+P GN NRV+I +  GVY EK+ +D A+PF+TL G P     LT+
Sbjct: 72  KGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAETVLTY 131

Query: 132 DGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQ--QALAARLMGDKAAVYN 191
            G A +YGTV SATLIV ++YF AA+L I NT+P P  KPG   QALA R+  DKAA Y+
Sbjct: 132 HGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMP--KPGSQGQALAMRINADKAAFYS 191

Query: 192 CKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITAHSR 251
           C+F+GFQDTLCDD G H FKDCYI+GT DFIFGRG SLYLN++L  +GDG L+VITA  R
Sbjct: 192 CRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDG-LRVITAQGR 251

Query: 252 EEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRHHGF 311
           +  ++ +GY+FVHC + GT G   YLGR+W    K ++++T +  +++P GW +  + G+
Sbjct: 252 QSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNRGY 311

Query: 312 DATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
           D TVF+ E+KC GPG+    RV Y +++   EV P+LTL +I+   WLLPPP
Sbjct: 312 DKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPP 359

BLAST of MS010139 vs. ExPASy TrEMBL
Match: A0A6J1DHQ9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020589 PE=4 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 1.6e-216
Identity = 366/366 (100.00%), Postives = 366/366 (100.00%), Query Frame = 0

Query: 1   MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA 60
           MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA
Sbjct: 1   MKYWGGIKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAA 60

Query: 61  EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY 120
           EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY
Sbjct: 61  EKNAKVVKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLY 120

Query: 121 GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL 180
           GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL
Sbjct: 121 GSPANVPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQQALAARL 180

Query: 181 MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK 240
           MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK
Sbjct: 181 MGDKAAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGK 240

Query: 241 LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG 300
           LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG
Sbjct: 241 LKVITAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEG 300

Query: 301 WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP 360
           WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP
Sbjct: 301 WHDMRHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPP 360

Query: 361 PTDPKA 367
           PTDPKA
Sbjct: 361 PTDPKA 366

BLAST of MS010139 vs. ExPASy TrEMBL
Match: A0A6J1DHP7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020595 PE=4 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 268/360 (74.44%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVV 67
           +L+ LLA      I   SPP+P DK+++N WFS+NVKPLA R+AELDP LVAAE+ A VV
Sbjct: 12  RLLTLLATF--ALILAASPPVPADKSQVNKWFSDNVKPLADRKAELDPALVAAEEGATVV 71

Query: 68  KVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVP 127
           KV ADGSGDFKT+TEAINSVP GNT RVVIWIGGGVYKEKL ++R KPFVTLYGSP N+P
Sbjct: 72  KVRADGSGDFKTVTEAINSVPTGNTKRVVIWIGGGVYKEKLKIERTKPFVTLYGSPNNMP 131

Query: 128 NLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKAA 187
           NLTFDG A K+GTVYSATL VESDYFTAANL I+N+SPRPDGK  G QALAARLMG KAA
Sbjct: 132 NLTFDGDANKFGTVYSATLTVESDYFTAANLIIVNSSPRPDGKRKGAQALAARLMGTKAA 191

Query: 188 VYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITA 247
           VYNCKF GFQDTLCDD GLHL+KDC+IQGT+DFIFG+GTSLYLNS+LDV+GDG L VITA
Sbjct: 192 VYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSLYLNSQLDVVGDGGLAVITA 251

Query: 248 HSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRH 307
           HSREEESD SGYSFVH SI GTGG+NTYLGRAW  RS+T+F+YTT+ADI++PEGW+DM+H
Sbjct: 252 HSREEESDPSGYSFVHGSITGTGGKNTYLGRAWMARSRTVFAYTTMADIINPEGWNDMKH 311

Query: 308 HGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPTDPKA 367
            GFD+TV F E+KC+GPG++ +GRVKY K+L+D EVKP+++L F+QSDKWLLPPP +P+A
Sbjct: 312 PGFDSTVLFGEYKCSGPGSEPSGRVKYSKQLSDVEVKPFVSLGFLQSDKWLLPPP-NPEA 368

BLAST of MS010139 vs. ExPASy TrEMBL
Match: A0A0A0KEH2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 4.8e-149
Identity = 258/358 (72.07%), Postives = 300/358 (83.80%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGR-EAELDPKLVAAEKNAKV 67
           +LV  LA      I  VSPPIP +K++L  WF+ENVKP A R +AELDP L  AE+NA V
Sbjct: 12  RLVTFLATF--TAILAVSPPIPVEKSQLAGWFTENVKPFAVRNKAELDPALATAEENATV 71

Query: 68  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGS-PAN 127
           +KVM+DG+G+FKT+TEAI SVP  N  RVVIWIG GVYKEKL +DR KPFVTLYGS P N
Sbjct: 72  IKVMSDGTGNFKTVTEAIASVPADNKKRVVIWIGVGVYKEKLKIDRNKPFVTLYGSDPKN 131

Query: 128 VPNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGDK 187
           +P LTFDG A KYGTVYSATLIVE+DYFTAANL I N+SPRPDG + G QALAAR MG K
Sbjct: 132 MPKLTFDGDAAKYGTVYSATLIVEADYFTAANLIIENSSPRPDGVRKGAQALAARFMGTK 191

Query: 188 AAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVI 247
           AA+YNCKF GFQDTLCDD GLH +KDC+IQGT+DFIFG+GTSLYLN++LDV GDG L VI
Sbjct: 192 AAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYLNTQLDVAGDGGLAVI 251

Query: 248 TAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDM 307
           TAHSRE+E+DTSGYSFVHCSI GTGG+NTYLGRAW PRS+ +F+YTTIADI+HPEGW+DM
Sbjct: 252 TAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVFAYTTIADIIHPEGWNDM 311

Query: 308 RHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPT 363
           +H GFD TV F E+KC+GPGA  TGRV YGK+LTD EVKP+L L+++QS+KWLLPPP+
Sbjct: 312 KHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLGLEYVQSEKWLLPPPS 367

BLAST of MS010139 vs. ExPASy TrEMBL
Match: A0A5A7V7V3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 6.5e-146
Identity = 248/355 (69.86%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVV 67
           +LV  LA      I  VS PIP +K+++++WFS+NVKPLA R+AELDP LVAAE+NA ++
Sbjct: 12  RLVTFLATF--TTILAVSTPIPVEKSQIDAWFSQNVKPLADRKAELDPALVAAEENATII 71

Query: 68  KVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVP 127
           KVM+DG+GDFKTITEAI SVP  N  RV+I IG G+YKEKL +DR KPFVTLYGSP  +P
Sbjct: 72  KVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYGSPKGMP 131

Query: 128 NLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGDKAA 187
            LTFDG A K+GTVYSATLIVE+DYFTAANL I N+SPRPDG + G QALAAR MG KAA
Sbjct: 132 KLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARFMGTKAA 191

Query: 188 VYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITA 247
           +YNCKF GFQDTLCDD GLH +KDC+IQGT+DFIFG+GTSLY+N++LDV GDG L VITA
Sbjct: 192 IYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDGGLAVITA 251

Query: 248 HSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRH 307
           HSRE+ESD SGYSFVHCSI GTGG+NT+LGRAW PRS+ +F+YTT+ADI+HPEGW+DM+H
Sbjct: 252 HSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPEGWNDMKH 311

Query: 308 HGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
            GFD TV F E+KC+GPG+  T RV YGK+L+D EV P+L+L ++QS+KWLLPPP
Sbjct: 312 AGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLPPP 364

BLAST of MS010139 vs. ExPASy TrEMBL
Match: A0A1S4DXM9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 6.5e-146
Identity = 248/355 (69.86%), Postives = 298/355 (83.94%), Query Frame = 0

Query: 8   KLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVV 67
           +LV  LA      I  VS PIP +K+++++WFS+NVKPLA R+AELDP LVAAE+NA ++
Sbjct: 8   RLVTFLATF--TTILAVSTPIPVEKSQIDAWFSQNVKPLADRKAELDPALVAAEENATII 67

Query: 68  KVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVP 127
           KVM+DG+GDFKTITEAI SVP  N  RV+I IG G+YKEKL +DR KPFVTLYGSP  +P
Sbjct: 68  KVMSDGTGDFKTITEAIASVPADNKKRVMISIGDGIYKEKLKIDRNKPFVTLYGSPKGMP 127

Query: 128 NLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDG-KPGQQALAARLMGDKAA 187
            LTFDG A K+GTVYSATLIVE+DYFTAANL I N+SPRPDG + G QALAAR MG KAA
Sbjct: 128 KLTFDGDAAKFGTVYSATLIVEADYFTAANLVIENSSPRPDGVRKGAQALAARFMGTKAA 187

Query: 188 VYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITA 247
           +YNCKF GFQDTLCDD GLH +KDC+IQGT+DFIFG+GTSLY+N++LDV GDG L VITA
Sbjct: 188 IYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSLYVNTQLDVAGDGGLAVITA 247

Query: 248 HSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRH 307
           HSRE+ESD SGYSFVHCSI GTGG+NT+LGRAW PRS+ +F+YTT+ADI+HPEGW+DM+H
Sbjct: 248 HSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVFAYTTMADIIHPEGWNDMKH 307

Query: 308 HGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
            GFD TV F E+KC+GPG+  T RV YGK+L+D EV P+L+L ++QS+KWLLPPP
Sbjct: 308 AGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLSLAYVQSEKWLLPPP 360

BLAST of MS010139 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 383.6 bits (984), Expect = 1.7e-106
Identity = 184/356 (51.69%), Postives = 247/356 (69.38%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I L +L+  V P+       PIP  K ++  WF+ NV PLA R+  LDP LVAAE   ++
Sbjct: 8   ILLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQRKG-LDPALVAAEAAPRI 67

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V   G G+FKT+T+AI SVP GNT RV+I +  G YKEK+ +DR KPF+TL G P  +
Sbjct: 68  INVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFITLMGQPNAM 127

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P +T+DG A KYGTV SA+LI+ SDYF A N+ + NT+P PDGK  G QAL+ R+ G+ A
Sbjct: 128 PVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFA 187

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYGFQDT+CDD G H FKDCY++GT DFIFG GTS+YL ++L V+GDG ++VI 
Sbjct: 188 AFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG-IRVIA 247

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +   + SGYSFVHC + GTGG   YLGRAW    K +++YT +  +++P GW + +
Sbjct: 248 AHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 307

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
               D TVF+ E+KC+GPG+ +  RV + +++ DKE   +L+L +IQ  KWLLPPP
Sbjct: 308 TPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLLPPP 359

BLAST of MS010139 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 3.8e-106
Identity = 186/357 (52.10%), Postives = 243/357 (68.07%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           + L+V  A+  P+ +     PIP D+ ++  WF  NVKP + R   LDP+L AAE + +V
Sbjct: 9   VALLVFFAS--PVVLADDITPIPADRAQIPQWFMANVKPFSQRRGTLDPELEAAEASRRV 68

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V  +G GDFKTI  AI S+P  N NRV+I +  G+Y EK+ VD  +P+VTL G P   
Sbjct: 69  IIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYVTLLGKPGAE 128

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQ--QALAARLMGDK 186
            NLT+ G A KYGTV SATLIV +  F AANLNIINTSP P  KPG   QALA R+ GDK
Sbjct: 129 TNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMP--KPGTQGQALAMRINGDK 188

Query: 187 AAVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVI 246
           AA YNC+FYGFQDTLCDD G H FK+CYI+GT DFIFGRG SLYL ++L  +GDG L+VI
Sbjct: 189 AAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAVGDG-LRVI 248

Query: 247 TAHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDM 306
            AH+R+  ++ +GYSFVHC + G  G   YLGRAW    K ++SYT ++ +++P GW + 
Sbjct: 249 AAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMSSVVNPSGWQEN 308

Query: 307 RHHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
           R    D TVF+ E+ CTGPG+ +  RV + +++ +KE   +LTL +I+  KWLLPPP
Sbjct: 309 RVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWLLPPP 359

BLAST of MS010139 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 378.6 bits (971), Expect = 5.5e-105
Identity = 182/356 (51.12%), Postives = 247/356 (69.38%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I L +L+  V P+       PIP  K ++  WF+ +V PLA R+  LDP LVAAE   ++
Sbjct: 8   ILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQRKG-LDPALVAAEAAPRI 67

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
           + V   G G+FKT+T+AI SVP GNT RV+I +  G Y+EK+ +DR KPF+TL G P  +
Sbjct: 68  INVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFITLMGQPNAM 127

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P +T+DG A KYGTV SA+LI+ SDYF A N+ + NT+P PDGK  G QAL+ R+ G+ A
Sbjct: 128 PVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALSMRISGNFA 187

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYGFQDT+CDD G H FKDCY++GT DFIFG GTS+YL ++L V+GDG ++VI 
Sbjct: 188 AFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVGDG-IRVIA 247

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +   + SGYSFVHC + GTGG   YLGRAW    K +++YT +  +++P GW + +
Sbjct: 248 AHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVYAYTEMTSVVNPTGWQENK 307

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
               D TVF+ E+KC+GPG+ +  RV + +++ DKE   +L+L +IQ  KWLLPPP
Sbjct: 308 TPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLLPPP 359

BLAST of MS010139 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.0e-103
Identity = 180/357 (50.42%), Postives = 240/357 (67.23%), Query Frame = 0

Query: 7   IKLVVLLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKV 66
           I  ++L+    P+     + PIP +K ++  WF+ NVK                      
Sbjct: 9   IVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTNVK---------------------- 68

Query: 67  VKVMADGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANV 126
                +G G FKTITEAINSV  GNT RV+I IG GVYKEK+ +DR+KPF+TLYG P  +
Sbjct: 69  ----QNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFITLYGHPNAM 128

Query: 127 PNLTFDGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGK-PGQQALAARLMGDKA 186
           P LTFDG A +YGTV SATLIV SDYF A N+ + N++P PDGK  G QAL+ R+ G+KA
Sbjct: 129 PVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQALSMRISGNKA 188

Query: 187 AVYNCKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVIT 246
           A YNCKFYG+QDT+CDD G H FKDCYI+GT DFIFG G SLYL ++L+V+GDG ++VIT
Sbjct: 189 AFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVVGDG-IRVIT 248

Query: 247 AHSREEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMR 306
           AH+ +  ++ SGYSFVHC + GT G   YLGR+W    K +++YT ++ +++P GW + R
Sbjct: 249 AHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVYAYTDMSSVVNPSGWQENR 308

Query: 307 HHGFDATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPPT 363
             G D TVF+ E+KCTG G+ +  RVKY +++ D E K +++L +IQ   WLLPPP+
Sbjct: 309 EAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWLLPPPS 337

BLAST of MS010139 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 368.2 bits (944), Expect = 7.5e-102
Identity = 174/352 (49.43%), Postives = 238/352 (67.61%), Query Frame = 0

Query: 12  LLAAVLPICIYGVSPPIPTDKTKLNSWFSENVKPLAGREAELDPKLVAAEKNAKVVKVMA 71
           L+    P+ +   + PIP ++ ++  WF  NVKP + R+  LDP L AAE   +++ V  
Sbjct: 12  LVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKGTLDPALEAAEAARQIITVNQ 71

Query: 72  DGSGDFKTITEAINSVPKGNTNRVVIWIGGGVYKEKLLVDRAKPFVTLYGSPANVPNLTF 131
            G  +FKT+ EAI S+P GN NRV+I +  GVY EK+ +D A+PF+TL G P     LT+
Sbjct: 72  KGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFITLLGQPGAETVLTY 131

Query: 132 DGVAKKYGTVYSATLIVESDYFTAANLNIINTSPRPDGKPGQ--QALAARLMGDKAAVYN 191
            G A +YGTV SATLIV ++YF AA+L I NT+P P  KPG   QALA R+  DKAA Y+
Sbjct: 132 HGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMP--KPGSQGQALAMRINADKAAFYS 191

Query: 192 CKFYGFQDTLCDDAGLHLFKDCYIQGTIDFIFGRGTSLYLNSELDVLGDGKLKVITAHSR 251
           C+F+GFQDTLCDD G H FKDCYI+GT DFIFGRG SLYLN++L  +GDG L+VITA  R
Sbjct: 192 CRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAVGDG-LRVITAQGR 251

Query: 252 EEESDTSGYSFVHCSIVGTGGRNTYLGRAWKPRSKTIFSYTTIADILHPEGWHDMRHHGF 311
           +  ++ +GY+FVHC + GT G   YLGR+W    K ++++T +  +++P GW +  + G+
Sbjct: 252 QSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMTSVVNPSGWRENLNRGY 311

Query: 312 DATVFFAEHKCTGPGADRTGRVKYGKELTDKEVKPYLTLDFIQSDKWLLPPP 362
           D TVF+ E+KC GPG+    RV Y +++   EV P+LTL +I+   WLLPPP
Sbjct: 312 DKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWLLPPP 359