MS010099 (gene) Bitter gourd (TR) v1

BLAST of MS010099 vs. NCBI nr
Match: XP_022133446.1 (SHUGOSHIN 2 [Momordica charantia])

HSP 1 Score: 690.6 bits (1781), Expect = 7.6e-195
Identity = 389/459 (84.75%), Postives = 391/459 (85.19%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           V GPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYE VDKL
Sbjct: 14  VGGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL
Sbjct: 74  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQ------------EVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGILLSRKLDQ            EVGTAECNEAEESMHTNKDTRPCKANR
Sbjct: 134 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
           PR+SKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ
Sbjct: 194 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSH ID SQCKETSVIQ
Sbjct: 254 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 313

Query: 301 TEVQKIED---------------------------------------------------- 360
           TEVQK+ED                                                    
Sbjct: 314 TEVQKVEDKRFCKRRQSARFRTDEPLATKDLFEIENSHSIAASQCEETSVLQTGVQKVEI 373

Query: 361 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 396
           KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD
Sbjct: 374 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 433

BLAST of MS010099 vs. NCBI nr
Match: XP_038884301.1 (SHUGOSHIN 2 [Benincasa hispida])

HSP 1 Score: 518.5 bits (1334), Expect = 5.2e-143
Identity = 294/411 (71.53%), Postives = 332/411 (80.78%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           V G NMKI GEK +KSSKVGG QRKRLSDISNLKEQPTLQKRDT  Q SLLMT+EYVDKL
Sbjct: 14  VGGQNMKIAGEKTMKSSKVGGGQRKRLSDISNLKEQPTLQKRDTMPQPSLLMTHEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQ NSQMLAELNSGK+RL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQANSQMLAELNSGKERL 133

Query: 121 KALQHELGCKNGILLSRKLD------------QEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKLD             EVGT EC+EAEES++ N+D RPCK NR
Sbjct: 134 KALQHELGCKNGILMSRKLDLERKGKSATLEPGEVGTTECSEAEESINANQDNRPCKTNR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            R+S+RES GTS LQ EV +VE KRPC+ RQ+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 RRQSRRESFGTSFLQKEVPRVEGKRPCLRRQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSV QTEVQK E KRPC+RRQS+RFK EEPVA  DLF+IE+S+  D SQCKE SV+Q
Sbjct: 254 CKETSVHQTEVQKVEGKRPCSRRQSARFKAEEPVAANDLFQIEDSNSTDASQCKEISVLQ 313

Query: 301 TEVQKIEDKRPCLRRQSARFKTEEPVAKTEDLV---SSSTSASSLYKEVVCEIAP-TAVS 360
           T+VQK+E KRPCLRRQS  FK +EPVA  + +    S+STS     +EV+CE+      S
Sbjct: 314 TDVQKVEGKRPCLRRQSTGFKLKEPVAVKDSVEIENSNSTSTFLCKEEVMCEVGKIVPTS 373

Query: 361 SVGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           SV +E  GN+TD+ EVQECRR SVGRP RRAAEKV SYKEIP+NVKMRRQ+
Sbjct: 374 SVEKEVYGNSTDRSEVQECRRISVGRPSRRAAEKVISYKEIPLNVKMRRQI 423

BLAST of MS010099 vs. NCBI nr
Match: XP_008456540.1 (PREDICTED: shugoshin-1 isoform X1 [Cucumis melo] >TYJ99967.1 shugoshin-1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 514.6 bits (1324), Expect = 7.5e-142
Identity = 289/409 (70.66%), Postives = 331/409 (80.93%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYEYVDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKL------------DQEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL              EVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            R+S+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   + SQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKIEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QK+E KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MS010099 vs. NCBI nr
Match: XP_022992974.1 (SHUGOSHIN 2-like isoform X7 [Cucurbita maxima])

HSP 1 Score: 512.7 bits (1319), Expect = 2.8e-141
Identity = 291/405 (71.85%), Postives = 333/405 (82.22%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYEYVDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQE------------VGTAECNEAEESMHTNKDTRPCKANRPRKS 184
           HELGCKNGIL+SRKLD E            VGTA+ +EA ESM+T +D RPCK NR R+S
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  D SQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KIEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           K+E  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR V
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRRPV 420

BLAST of MS010099 vs. NCBI nr
Match: XP_022992970.1 (SHUGOSHIN 2-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 511.9 bits (1317), Expect = 4.9e-141
Identity = 290/403 (71.96%), Postives = 332/403 (82.38%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYEYVDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQE------------VGTAECNEAEESMHTNKDTRPCKANRPRKS 184
           HELGCKNGIL+SRKLD E            VGTA+ +EA ESM+T +D RPCK NR R+S
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  D SQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KIEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           K+E  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRR 394
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRR 418

BLAST of MS010099 vs. ExPASy Swiss-Prot
Match: Q0WTB8 (SHUGOSHIN 2 OS=Arabidopsis thaliana OX=3702 GN=SGO2 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.8e-34
Identity = 153/483 (31.68%), Postives = 235/483 (48.65%), Query Frame = 0

Query: 6   MKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLM-TYEYVDKLQKEN 65
           M +T   +  S++V GSQ+       N+K+   + K +T+Q+ ++L  + EY  KLQKEN
Sbjct: 1   MVLTEMSLDYSNRVLGSQK------DNVKQ---MDKEETQQKENMLFSSQEYAAKLQKEN 60

Query: 66  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 125
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 61  MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 120

Query: 126 HELGCKNGILLSRK-LDQEVGTAECNEAEESMHTNKDTRPCKA-------NRPRKSKRES 185
           HELGCKN +L  +K L+++V     +E+++ +  +     CK+       ++  K KR +
Sbjct: 121 HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 180

Query: 186 SGTSVLQTEVQ--QVEDKRPCVTRQTARFKTEEPVASTKELSEIEN-----FNSTDFSQC 245
              S +  +V+   V D      R+       +     +E+ + E+       S   +Q 
Sbjct: 181 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTEDDIDKGVVSRGVNQD 240

Query: 246 KETSVVQTEVQKAES--------KRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQC 305
            +  V +  V  A +        KR C RRQS+RF ++E   T+ L E++ +      + 
Sbjct: 241 IDNVVNKKFVPDAANPVKESVHRKRQCTRRQSTRFDVQETKQTEKLLEMDGA-----KES 300

Query: 306 KETSVIQ----------------------TEVQKIEDKRPCLRRQSARFKTEEP----VA 365
           KET+                          EV++   +R    R SARF  +EP     +
Sbjct: 301 KETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLRLSARFDIQEPHVTETS 360

Query: 366 KTEDLVS------------------------------------SSTSASSLYKEVVCEIA 395
             +D  S                                     ST   S   E + EIA
Sbjct: 361 NADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQSTKGKSQTDEAIKEIA 420

BLAST of MS010099 vs. ExPASy Swiss-Prot
Match: F4J3S1 (SHUGOSHIN 1 OS=Arabidopsis thaliana OX=3702 GN=SGO1 PE=3 SV=1)

HSP 1 Score: 121.7 bits (304), Expect = 1.9e-26
Identity = 123/411 (29.93%), Postives = 201/411 (48.91%), Query Frame = 0

Query: 7   KITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTK--QQSSLLMTYEYVDKLQKEN 66
           K  GEKM+    +  +QR++L DI+NL+ Q  L  +  K  QQ+ L+ + E  + LQKEN
Sbjct: 21  KAKGEKMVLEPPMNSAQRRKLGDITNLQNQKNLMNQGAKHQQQAILISSKENAENLQKEN 80

Query: 67  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 126
             LMK++ ER+ I     ++L+KLR  FQK+Q+QNL LAQ N+++LAE N+ KD+LK LQ
Sbjct: 81  TKLMKVVMERDGI----KSDLKKLRIEFQKVQEQNLLLAQANTRILAEFNTSKDQLKVLQ 140

Query: 127 HELGCKNGILLSRKLDQEVGTAEC--NEAEESMHTNKDTRPCKANRPRKSKRE----SSG 186
           HELGCKNG++++RK+  +  T  C  + ++     N     CK  +P  +  E    SS 
Sbjct: 141 HELGCKNGLVMARKMLLKEQTLPCTRHASKVKAQANACGGACKTFQPNDADHEHASGSSN 200

Query: 187 TSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIE-NFNSTDFSQCKETSVVQT 246
            + LQ   +    +R    +  A  +  + +  + E  ++E N ++       +      
Sbjct: 201 ANSLQRNEKANSKRRVSGRKNPANSEVLDIIGRSGETCQMEDNIDNKKLVSDSDNDAENH 260

Query: 247 EVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLID----VSQCKETSVIQTE--- 306
                +SKR C  RQSS  K  E   T+ L ++ ++  I      S  K +  ++++   
Sbjct: 261 INDNVQSKRYCAGRQSSSSKTREASQTETLQKVVDAKEIKGDARFSLTKHSDWLKSQEPE 320

Query: 307 -VQKIEDKRPCLRRQSARFKTEEPVAKT--EDLVSSSTSASSLYKEVVCEIAPTAV--SS 366
             + + + R  LRR+SAR K++EP       D + ++    S  +  +  I    V  + 
Sbjct: 321 PSESLYESRFPLRRRSARLKSQEPEPSESFHDSIETTKRRRSAIRSAMFNIQELGVIQNL 380

Query: 367 VGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEI--PINVKMRRQ 395
            G  DD     K       +S+  +P    A +    KEI     + +RRQ
Sbjct: 381 NGLPDDQEIAAKARCSAREQSTGSKP---EAVEPHDTKEIIGKSRISLRRQ 424

BLAST of MS010099 vs. ExPASy Swiss-Prot
Match: E3VXF2 (Shugoshin-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SGO1 PE=2 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.9e-19
Identity = 121/435 (27.82%), Postives = 186/435 (42.76%), Query Frame = 0

Query: 62  KENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLK 121
           KEN  L  L+AERN++IE+S  EL+K+R   Q +QQ+NLQL Q NSQM AE+N GKDR+K
Sbjct: 55  KENAKLRHLLAERNKVIEVSRVELQKIRLALQAMQQKNLQLVQANSQMFAEINQGKDRIK 114

Query: 122 ALQHELGCKNGILLSRKLD-QEVGTAECNEAEESMHTNKDTRPCKANRPRKSKRESSGTS 181
            LQHEL C   +L  +  + +++     N+   +    K TR  K   P K+ + ++G+ 
Sbjct: 115 LLQHELACTIAVLKVKGSELEKMSKTSNNQQNRAKILEKKTRSSKC-APTKAHQMAAGS- 174

Query: 182 VLQTEVQQVEDKRPCVT--RQTARFKTEEPVASTK--ELSEIENFNSTDFSQCKETSVVQ 241
            ++  + +++   P  T   +  + KT +   + +  E  E+    +T    C+   V  
Sbjct: 175 -IREHLVEIQSAVPSYTSCHEPPQDKTNKRCTNRRKSESCEVTMDTNTVQHSCR-PHVEY 234

Query: 242 TEVQKAESKRPCNRRQSSRF-----------------------------KIEEPVATKDL 301
                 +  R   RR+S+R                              K++EP A KD+
Sbjct: 235 NGSSHDDDPRKTRRRRSARLNPGSFEVAEICDKLHEDATVPSAPSSNVPKLQEPNAGKDM 294

Query: 302 F----------EIENSHLIDVSQCKETSVIQT--------EVQKIEDKRPCLRRQSARFK 361
                      E+    +  V +  E   IQ         E  K + + P   R+S R  
Sbjct: 295 ICGGKMKSLQKELPCDAIAQVVEAPELKEIQEAGSSVAGGEAHKFDIEDPEPPRKSMRID 354

Query: 362 TEEP---------VAKTEDLVSS---STSASSLYK------------------------- 394
             +           +  ED +++   STS+  +                           
Sbjct: 355 ANKRKLESFESRLASNKEDCINAICDSTSSVPIQHEQKRKLSRRKSSRLDPGPWEVTNGT 414

BLAST of MS010099 vs. ExPASy Swiss-Prot
Match: Q4QSC8 (Shugoshin-1 OS=Zea mays OX=4577 GN=SGO1 PE=2 SV=2)

HSP 1 Score: 84.7 bits (208), Expect = 2.5e-15
Identity = 132/469 (28.14%), Postives = 195/469 (41.58%), Query Frame = 0

Query: 10  GEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKLQKENMTLMK 69
           G   I+S    G++   L+DI+N   +P   +  T            V  L KEN  L+ 
Sbjct: 25  GGPRIRSPPGKGNKPVALADITN-TGKPNAARSIT------------VPDLVKENTKLLT 84

Query: 70  LIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQHELGC 129
           L+ E+ +II++S  E+ KLR   Q  +QQNL L QTNSQMLAE+N+GKDR+K LQHEL C
Sbjct: 85  LLNEKTKIIDLSRVEIYKLRLALQASKQQNLHLTQTNSQMLAEINTGKDRIKMLQHELSC 144

Query: 130 KNGIL------LSRKLD-----------QEVGTAECNEAEESMHTNKDTRPCKANRPRKS 189
              +L      L RK +           Q + T     A E+ H          +   +S
Sbjct: 145 TTALLKVKDSELDRKKNAGNVQQKGVKSQVLKTKASTVAVEAHHVGDSVTSGVEHHVVES 204

Query: 190 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKT--------------EEPV--------- 249
           +   S  +V Q   Q  + KR    R+++R                 E PV         
Sbjct: 205 QSAVSSNTVCQEPPQDGKQKRMPQRRRSSRLNQGSCEIRGVSQNTLHENPVVPVAPSTLS 264

Query: 250 -----------------------------ASTKELSEIENF-NSTDFSQCKETSVVQTEV 309
                                        AS  E +EI      TD  +  E    +TEV
Sbjct: 265 LEKQYGQTTGKHMKSLQNECSATVHEVIMASEFEKTEINELPQKTDLKEIPEACSSETEV 324

Query: 310 QKAE-SKRPCNRRQSSRFKIEEPVA--TKDLFEIENSHLIDVSQCKETSV---------I 369
           Q  +   +  N +Q+     +  ++  T D  E    + +     K +S+         I
Sbjct: 325 QSHKIGDKAFNSKQNHLTGSQSSLSFNTVDTPEPPEDNTVKRCSKKRSSIEDVNAKLDTI 384

Query: 370 QTEVQKIEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTA--VSS 394
            +E  + E+KR   R+ SAR  +    ++  D+V       + +K+V+  +A +   VS 
Sbjct: 385 TSEPLRHEEKRKSRRKISARLNSVS--SEHTDIV-----VETEHKDVIVSLAGSTSNVSM 444

BLAST of MS010099 vs. ExPASy TrEMBL
Match: A0A6J1BV49 (SHUGOSHIN 2 OS=Momordica charantia OX=3673 GN=LOC111006020 PE=3 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 3.7e-195
Identity = 389/459 (84.75%), Postives = 391/459 (85.19%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           V GPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYE VDKL
Sbjct: 14  VGGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYECVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL
Sbjct: 74  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 133

Query: 121 KALQHELGCKNGILLSRKLDQ------------EVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGILLSRKLDQ            EVGTAECNEAEESMHTNKDTRPCKANR
Sbjct: 134 KALQHELGCKNGILLSRKLDQEIKEKSAALQAGEVGTAECNEAEESMHTNKDTRPCKANR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
           PR+SKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ
Sbjct: 194 PRQSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSH ID SQCKETSVIQ
Sbjct: 254 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHFIDASQCKETSVIQ 313

Query: 301 TEVQKIED---------------------------------------------------- 360
           TEVQK+ED                                                    
Sbjct: 314 TEVQKVEDKRFCKRRQSARFRTDEPLATKDLFEIENSHSIAASQCEETSVLQTGVQKVEI 373

Query: 361 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 396
           KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD
Sbjct: 374 KRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLYKEVVCEIAPTAVSSVGREDDGNATD 433

BLAST of MS010099 vs. ExPASy TrEMBL
Match: A0A5D3BLG7 (Shugoshin-1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00080 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.6e-142
Identity = 289/409 (70.66%), Postives = 331/409 (80.93%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYEYVDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKL------------DQEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL              EVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            R+S+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   + SQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKIEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QK+E KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MS010099 vs. ExPASy TrEMBL
Match: A0A1S3C330 (shugoshin-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496463 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 3.6e-142
Identity = 289/409 (70.66%), Postives = 331/409 (80.93%), Query Frame = 0

Query: 1   VVGPNMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKL 60
           VVG N+KI GEK +KSSK    QRKRLSDISNLKEQPTLQKRDTK Q  LLMTYEYVDKL
Sbjct: 14  VVGQNVKIAGEKTMKSSK---GQRKRLSDISNLKEQPTLQKRDTKTQPGLLMTYEYVDKL 73

Query: 61  QKENMTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRL 120
           QKENMTLMK+IAERNRIIEISGNELEKLRTNFQKLQQQN+QLAQ N QMLAELNS KDRL
Sbjct: 74  QKENMTLMKVIAERNRIIEISGNELEKLRTNFQKLQQQNMQLAQANCQMLAELNSSKDRL 133

Query: 121 KALQHELGCKNGILLSRKL------------DQEVGTAECNEAEESMHTNKDTRPCKANR 180
           KALQHELGCKNGIL+SRKL              EVGT ECNEAEES+  N+D RPCK+NR
Sbjct: 134 KALQHELGCKNGILMSRKLVLESKGKSATLQPGEVGTTECNEAEESISANQDNRPCKSNR 193

Query: 181 PRKSKRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQ 240
            R+S+RES GTS LQTEV ++E KRPC+ +Q+A+FKTEEPVA+  ++ E EN NS D SQ
Sbjct: 194 KRQSRRESFGTSSLQTEVPKIEGKRPCLRQQSAKFKTEEPVAA-NDILETENSNSNDASQ 253

Query: 241 CKETSVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQ 300
           CKETSV+QTEVQK E KRPC+RRQS+RFK EEPV T DL +IE S   + SQC+ETSV+Q
Sbjct: 254 CKETSVLQTEVQKVEGKRPCSRRQSARFKAEEPVNTNDLQQIETSDSTNASQCQETSVLQ 313

Query: 301 TEVQKIEDKRPCLRRQSARFKTEEPVAKTEDLVSSSTSASSLY--KEVVCEIAPTAVSSV 360
            E+QK+E KRPCLRRQS RFK EEPVA  + L   +++++S +  KE +CE+ PT  SSV
Sbjct: 314 AEIQKVEGKRPCLRRQSTRFKLEEPVATKDSLEIENSNSTSAFPCKETMCEVVPT--SSV 373

Query: 361 GREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           G+ED  N+ D+ EVQECRR+SVGRP RRAAEKV SYKEIP+NVKMRRQV
Sbjct: 374 GKEDYDNSIDRSEVQECRRTSVGRPSRRAAEKVISYKEIPVNVKMRRQV 416

BLAST of MS010099 vs. ExPASy TrEMBL
Match: A0A6J1JYY1 (SHUGOSHIN 2-like isoform X7 OS=Cucurbita maxima OX=3661 GN=LOC111489140 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.4e-141
Identity = 291/405 (71.85%), Postives = 333/405 (82.22%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYEYVDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQE------------VGTAECNEAEESMHTNKDTRPCKANRPRKS 184
           HELGCKNGIL+SRKLD E            VGTA+ +EA ESM+T +D RPCK NR R+S
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  D SQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KIEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           K+E  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRRQV 396
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR V
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRRPV 420

BLAST of MS010099 vs. ExPASy TrEMBL
Match: A0A6J1JX77 (SHUGOSHIN 2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111489140 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 2.4e-141
Identity = 290/403 (71.96%), Postives = 332/403 (82.38%), Query Frame = 0

Query: 5   NMKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLMTYEYVDKLQKEN 64
           NM+I G K +KSSKVGGSQRKRLSDISNLKEQP LQKRDTK QSSLL TYEYVDKLQKEN
Sbjct: 19  NMEIAGVKTMKSSKVGGSQRKRLSDISNLKEQPILQKRDTKPQSSLLKTYEYVDKLQKEN 78

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           M L K++AERNRIIEISGNELE LRTNFQKLQQQNLQ AQ NSQMLAELNSGK+RLKALQ
Sbjct: 79  MALTKVLAERNRIIEISGNELETLRTNFQKLQQQNLQFAQANSQMLAELNSGKERLKALQ 138

Query: 125 HELGCKNGILLSRKLDQE------------VGTAECNEAEESMHTNKDTRPCKANRPRKS 184
           HELGCKNGIL+SRKLD E            VGTA+ +EA ESM+T +D RPCK NR R+S
Sbjct: 139 HELGCKNGILMSRKLDLERKGKSATFQPGGVGTAKSSEAAESMNTKQDNRPCKTNRRRQS 198

Query: 185 KRESSGTSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIENFNSTDFSQCKET 244
           ++ES GTSVLQTEVQ+VE KRP   RQ+ARFKTEEPVA+  ++ E +N NS + SQCKET
Sbjct: 199 RQESFGTSVLQTEVQKVEGKRP--RRQSARFKTEEPVAA-NDILETDNSNSNNSSQCKET 258

Query: 245 SVVQTEVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQCKETSVIQTEVQ 304
           SV + EVQK E KRPC+RRQS+R KIEEPVAT DL  IEN +  D SQC+ETSV+QTEVQ
Sbjct: 259 SVRRAEVQKVEGKRPCSRRQSARLKIEEPVATNDLLGIENFNSTDASQCRETSVLQTEVQ 318

Query: 305 KIEDKRPCLRRQSARFKTEEPVAKTE--DLVSSSTSASSLYKEVVCEIAPTAVSSVGRED 364
           K+E  RPCLRRQSARFK EEPVA  +  D V+S+++++SL KE+VCEI     SSV  +D
Sbjct: 319 KVEGIRPCLRRQSARFKLEEPVAIKDSLDTVNSNSTSASLCKEIVCEIKIVPTSSVETKD 378

Query: 365 DGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEIPINVKMRR 394
           +GN+TD+ EVQECRR+SVGRPLRRAAEK+QSYKEIP+NVKMRR
Sbjct: 379 NGNSTDRSEVQECRRTSVGRPLRRAAEKIQSYKEIPLNVKMRR 418

BLAST of MS010099 vs. TAIR 10
Match: AT5G04320.2 (Shugoshin C terminus )

HSP 1 Score: 148.3 bits (373), Expect = 1.3e-35
Identity = 153/483 (31.68%), Postives = 235/483 (48.65%), Query Frame = 0

Query: 6   MKITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTKQQSSLLM-TYEYVDKLQKEN 65
           M +T   +  S++V GSQ+       N+K+   + K +T+Q+ ++L  + EY  KLQKEN
Sbjct: 1   MVLTEMSLDYSNRVLGSQK------DNVKQ---MDKEETQQKENMLFSSQEYAAKLQKEN 60

Query: 66  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 125
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 61  MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 120

Query: 126 HELGCKNGILLSRK-LDQEVGTAECNEAEESMHTNKDTRPCKA-------NRPRKSKRES 185
           HELGCKN +L  +K L+++V     +E+++ +  +     CK+       ++  K KR +
Sbjct: 121 HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 180

Query: 186 SGTSVLQTEVQ--QVEDKRPCVTRQTARFKTEEPVASTKELSEIEN-----FNSTDFSQC 245
              S +  +V+   V D      R+       +     +E+ + E+       S   +Q 
Sbjct: 181 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTEDDIDKGVVSRGVNQD 240

Query: 246 KETSVVQTEVQKAES--------KRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQC 305
            +  V +  V  A +        KR C RRQS+RF ++E   T+ L E++ +      + 
Sbjct: 241 IDNVVNKKFVPDAANPVKESVHRKRQCTRRQSTRFDVQETKQTEKLLEMDGA-----KES 300

Query: 306 KETSVIQ----------------------TEVQKIEDKRPCLRRQSARFKTEEP----VA 365
           KET+                          EV++   +R    R SARF  +EP     +
Sbjct: 301 KETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLRLSARFDIQEPHVTETS 360

Query: 366 KTEDLVS------------------------------------SSTSASSLYKEVVCEIA 395
             +D  S                                     ST   S   E + EIA
Sbjct: 361 NADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQSTKGKSQTDEAIKEIA 420

BLAST of MS010099 vs. TAIR 10
Match: AT5G04320.1 (Shugoshin C terminus )

HSP 1 Score: 127.9 bits (320), Expect = 1.8e-29
Identity = 132/423 (31.21%), Postives = 200/423 (47.28%), Query Frame = 0

Query: 65  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 124
           MTLMK +A RN+++E+SG E++KLR N + +Q++NLQLAQ NSQMLAELN+ +DRLK LQ
Sbjct: 1   MTLMKALAHRNKLVELSGIEIQKLRINLRSVQEKNLQLAQANSQMLAELNTNRDRLKDLQ 60

Query: 125 HELGCKNGILLSRK-LDQEVGTAECNEAEESMHTNKDTRPCKA-------NRPRKSKRES 184
           HELGCKN +L  +K L+++V     +E+++ +  +     CK+       ++  K KR +
Sbjct: 61  HELGCKNALLKVKKHLEEQVLPRTHHESKDKVSASASDGDCKSFQVHDIKHKDTKRKRTT 120

Query: 185 SGTSVLQTEVQ--QVEDKRPCVTRQTARFKTEEPVASTKELSEIEN-----FNSTDFSQC 244
              S +  +V+   V D      R+       +     +E+ + E+       S   +Q 
Sbjct: 121 RIKSSVSADVKPIPVNDSNSKANRKRRVSGVIDTTGIPEEICQTEDDIDKGVVSRGVNQD 180

Query: 245 KETSVVQTEVQKAES--------KRPCNRRQSSRFKIEEPVATKDLFEIENSHLIDVSQC 304
            +  V +  V  A +        KR C RRQS+RF ++E   T+ L E++ +      + 
Sbjct: 181 IDNVVNKKFVPDAANPVKESVHRKRQCTRRQSTRFDVQETKQTEKLLEMDGA-----KES 240

Query: 305 KETSVIQ----------------------TEVQKIEDKRPCLRRQSARFKTEEP----VA 364
           KET+                          EV++   +R    R SARF  +EP     +
Sbjct: 241 KETASFSLRRRSARLRHEEAEPCKSLHEGDEVRETIKRRRVSLRLSARFDIQEPHVTETS 300

Query: 365 KTEDLVS------------------------------------SSTSASSLYKEVVCEIA 395
             +D  S                                     ST   S   E + EIA
Sbjct: 301 NADDARSIVIEESAGSRSESVEPSESRHETKEITRKRSFSTRRQSTKGKSQTDEAIKEIA 360

BLAST of MS010099 vs. TAIR 10
Match: AT3G10440.1 (Shugoshin C terminus )

HSP 1 Score: 121.7 bits (304), Expect = 1.3e-27
Identity = 123/411 (29.93%), Postives = 201/411 (48.91%), Query Frame = 0

Query: 7   KITGEKMIKSSKVGGSQRKRLSDISNLKEQPTLQKRDTK--QQSSLLMTYEYVDKLQKEN 66
           K  GEKM+    +  +QR++L DI+NL+ Q  L  +  K  QQ+ L+ + E  + LQKEN
Sbjct: 21  KAKGEKMVLEPPMNSAQRRKLGDITNLQNQKNLMNQGAKHQQQAILISSKENAENLQKEN 80

Query: 67  MTLMKLIAERNRIIEISGNELEKLRTNFQKLQQQNLQLAQTNSQMLAELNSGKDRLKALQ 126
             LMK++ ER+ I     ++L+KLR  FQK+Q+QNL LAQ N+++LAE N+ KD+LK LQ
Sbjct: 81  TKLMKVVMERDGI----KSDLKKLRIEFQKVQEQNLLLAQANTRILAEFNTSKDQLKVLQ 140

Query: 127 HELGCKNGILLSRKLDQEVGTAEC--NEAEESMHTNKDTRPCKANRPRKSKRE----SSG 186
           HELGCKNG++++RK+  +  T  C  + ++     N     CK  +P  +  E    SS 
Sbjct: 141 HELGCKNGLVMARKMLLKEQTLPCTRHASKVKAQANACGGACKTFQPNDADHEHASGSSN 200

Query: 187 TSVLQTEVQQVEDKRPCVTRQTARFKTEEPVASTKELSEIE-NFNSTDFSQCKETSVVQT 246
            + LQ   +    +R    +  A  +  + +  + E  ++E N ++       +      
Sbjct: 201 ANSLQRNEKANSKRRVSGRKNPANSEVLDIIGRSGETCQMEDNIDNKKLVSDSDNDAENH 260

Query: 247 EVQKAESKRPCNRRQSSRFKIEEPVATKDLFEIENSHLID----VSQCKETSVIQTE--- 306
                +SKR C  RQSS  K  E   T+ L ++ ++  I      S  K +  ++++   
Sbjct: 261 INDNVQSKRYCAGRQSSSSKTREASQTETLQKVVDAKEIKGDARFSLTKHSDWLKSQEPE 320

Query: 307 -VQKIEDKRPCLRRQSARFKTEEPVAKT--EDLVSSSTSASSLYKEVVCEIAPTAV--SS 366
             + + + R  LRR+SAR K++EP       D + ++    S  +  +  I    V  + 
Sbjct: 321 PSESLYESRFPLRRRSARLKSQEPEPSESFHDSIETTKRRRSAIRSAMFNIQELGVIQNL 380

Query: 367 VGREDDGNATDKFEVQECRRSSVGRPLRRAAEKVQSYKEI--PINVKMRRQ 395
            G  DD     K       +S+  +P    A +    KEI     + +RRQ
Sbjct: 381 NGLPDDQEIAAKARCSAREQSTGSKP---EAVEPHDTKEIIGKSRISLRRQ 424