MS010097 (gene) Bitter gourd (TR) v1

BLAST of MS010097 vs. NCBI nr
Match: XP_022133448.1 (protein RNA-directed DNA methylation 3, partial [Momordica charantia])

HSP 1 Score: 2241.8 bits (5808), Expect = 0.0e+00
Identity = 1206/1317 (91.57%), Postives = 1215/1317 (92.26%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD
Sbjct: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN
Sbjct: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH
Sbjct: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI
Sbjct: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS
Sbjct: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK 600
            DFS SPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK
Sbjct: 541  DFSFSPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK 600

Query: 601  DVTVKLDSQQKVLTVRSDFLSEVYRKTSTV---EDTEFGSLKPFDILGNEGSSQDWMGGT 660
            DVTVKLDSQQKVLTVRSDFLSEVYRKTSTV   EDTEFGSLKPFDILGNEGSSQDWMGGT
Sbjct: 601  DVTVKLDSQQKVLTVRSDFLSEVYRKTSTVSLSEDTEFGSLKPFDILGNEGSSQDWMGGT 660

Query: 661  GSSTGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASM-IITDVEDSPWV 720
            GSSTGGDGWNSAGPSSERSPWPSFPESGTS     P SS + F   S+    DVEDSPWV
Sbjct: 661  GSSTGGDGWNSAGPSSERSPWPSFPESGTS---NCPGSSSNPFGSESLDAQKDVEDSPWV 720

Query: 721  SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG 780
            SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG
Sbjct: 721  SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG 780

Query: 781  GDY------------------------------------------KDSESGGWKKSQNAS 840
            GD                                           KDSESGGWKKSQNAS
Sbjct: 781  GDCAGPTDQAEDKWDKGKRVSSDNQTGNWGDGTSGKNEPSAWSRDKDSESGGWKKSQNAS 840

Query: 841  FGDENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN 900
            FGDENNSAEASA+KWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN
Sbjct: 841  FGDENNSAEASADKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN 900

Query: 901  TQKQESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGW 960
            TQKQESNR SWGKPSDRGGTRSSESGRGGNQVRGGS GQDSSSDWNKSTTTDGAGKKDGW
Sbjct: 901  TQKQESNRGSWGKPSDRGGTRSSESGRGGNQVRGGSSGQDSSSDWNKSTTTDGAGKKDGW 960

Query: 961  NKPNLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS 1020
            NKPNLASEDESIGKKGWGQGNEA+DSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS
Sbjct: 961  NKPNLASEDESIGKKGWGQGNEANDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS 1020

Query: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080
            SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG
Sbjct: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080

Query: 1081 KDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA 1140
            KDGERKD+GSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA
Sbjct: 1081 KDGERKDQGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA 1140

Query: 1141 EKSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG 1200
            +KSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG
Sbjct: 1141 KKSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG 1200

Query: 1201 GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWEKSGSDRGGFGGGRGRWSSGGG 1260
            GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWE                     
Sbjct: 1201 GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWE--------------------- 1260

Query: 1261 GSDSGWGKSGSDRGGFGGGRGRGRWNQGDSNSNDGGNGGWSSSGGRGGFGGGRGRGR 1272
                   KSGSDRGGFGGGRGRGRWNQ ++ SNDG +GGWS     GG GG RGRGR
Sbjct: 1261 -------KSGSDRGGFGGGRGRGRWNQ-EAGSNDGDSGGWS-----GGGGGHRGRGR 1280

BLAST of MS010097 vs. NCBI nr
Match: XP_023524523.1 (protein RNA-directed DNA methylation 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1909.4 bits (4945), Expect = 0.0e+00
Identity = 1156/1689 (68.44%), Postives = 1258/1689 (74.48%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKDTSSGKRK R+D + +  ARKRRDRSVLQFFEDV+PE+G  SDDSDFLDD
Sbjct: 1    MASKGKGIAKDTSSGKRKLRDDTD-SSAARKRRDRSVLQFFEDVSPELGAYSDDSDFLDD 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEEEF+  PAFKND +K QNIPFFPKEEEMNEEEFDR+MEEHY +GPGLGAFAEENYEN
Sbjct: 61   FMEEEFEPVPAFKNDDSKAQNIPFFPKEEEMNEEEFDRMMEEHYTRGPGLGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            K STGRNP Q S+RD + LWK+KCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAF V+H
Sbjct: 121  KISTGRNPPQQSARDIVFLWKLKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            +KGFIYIEAPRQYDLIEACKGI+GIYSTRIASVPENDISQLLTV+SRVSEV+VGTMARVK
Sbjct: 181  IKGFIYIEAPRQYDLIEACKGINGIYSTRIASVPENDISQLLTVQSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKL+PRIDLQ+MA KFGGGV AKK TNPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLIPRIDLQSMAEKFGGGVVAKKATNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            +EFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KK+SLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  EEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKVSLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNE+NDLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSSM SF DHDL+CFGRKDFGMI
Sbjct: 361  SNEANDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSMISFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LGTEKDDSYKILK+GPDGS+VVNVQRKELKSGPL+ KFT+ DHNGKIISVSDNVKVLEGS
Sbjct: 421  LGTEKDDSYKILKDGPDGSVVVNVQRKELKSGPLDAKFTSVDHNGKIISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSN+C KIKISYDAPSGK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNLCAKIKISYDAPSGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK+ GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCR IAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKE-GREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRAIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTV---EDTEFGSLKPFDILGNEGSSQDWMGG 660
            +DVTVKLDSQQKVLTVRSD L+EV+RK+S V   ED EF SLKPFDILGNEGSSQDWMGG
Sbjct: 601  RDVTVKLDSQQKVLTVRSDLLTEVHRKSSAVSVSEDPEFSSLKPFDILGNEGSSQDWMGG 660

Query: 661  TGSSTGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIIT--DVEDSP 720
            TGSS  GDGWNSAGPSSERSPWPSFPESGT      P SS ++ +    +    DVEDSP
Sbjct: 661  TGSSAAGDGWNSAGPSSERSPWPSFPESGT---LNCPGSSSTNPFGTENLDAKEDVEDSP 720

Query: 721  WVSKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVV 780
            WVSK+   A+TSWGAAKS+V DT NDDQA GWGKSESWDKATAKT SDGN SGAW K VV
Sbjct: 721  WVSKSTAEANTSWGAAKSSV-DTANDDQACGWGKSESWDKATAKTISDGNVSGAWGKSVV 780

Query: 781  PGGDY------------------------------------KDSESGGWKKSQNASFGDE 840
            P GD                                     KDSESGGWKK+ + S GD 
Sbjct: 781  PSGDSAGDKWDKEKHVTSDNQTGKWGGGTSGKNEFSEWSRDKDSESGGWKKNPSVSVGDN 840

Query: 841  NNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQKQ 900
            N  AE SA+KW SKNRS+G WGD N STTVSEI+ AGK N G                  
Sbjct: 841  NTPAETSADKWGSKNRSNGSWGDQNVSTTVSEIQTAGKGNVG------------------ 900

Query: 901  ESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKPN 960
                  W KP                    G+  + + S WN+ T+  G G+   W    
Sbjct: 901  -----GWTKP--------------------GAENKINPSGWNEDTSMKG-GQTSNW---- 960

Query: 961  LASEDESIGKKGWGQGNEASDSGNK-WQSSGS--DGGKKW-GTNESERDGGNKSWNTSKS 1020
              ++DE+    GWG+     D G+  W  S +  DG   W  +NE ER+GGN+SWN SKS
Sbjct: 961  -GNQDEA---GGWGKPMNVGDGGSSAWNKSTACGDGNDSWKRSNELEREGGNRSWNASKS 1020

Query: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080
            SDGD     SAIWKDKSDSSSLTA KGDQWGG W+KQHSSN+TKASEDNSPWNKKSVESG
Sbjct: 1021 SDGD-----SAIWKDKSDSSSLTASKGDQWGGGWNKQHSSNETKASEDNSPWNKKSVESG 1080

Query: 1081 KDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS-- 1140
            KD E +++GSGWNVGK+S GDSASGWGQTSKEAGSSDQ  SWGSNWKK S+ GNEDSS  
Sbjct: 1081 KDNEVENQGSGWNVGKTS-GDSASGWGQTSKEAGSSDQGSSWGSNWKKKSDTGNEDSSSA 1140

Query: 1141 -------------WAEKSNWNSGNESNDNQFT----GDTSGHGSWGGDGSDRGGFRGRGG 1200
                         W EKSNWNSGNE N N         T     W G+ SDRGGFRGRG 
Sbjct: 1141 KKSNWSSGSGNSNWGEKSNWNSGNEFNANHSNDGAEAQTEVSNDWRGESSDRGGFRGRGS 1200

Query: 1201 FRGRGERGRFGGRGRSD-----RGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG 1260
            FRGRGERGRFGGRGRSD     RGGSD GGFGGRGRGRWNSEGGSNDG+N+GWS  GGGG
Sbjct: 1201 FRGRGERGRFGGRGRSDRGGFGRGGSDGGGFGGRGRGRWNSEGGSNDGDNKGWS--GGGG 1260

Query: 1261 DWEKSGSDRGGFGG-GRGR--------------WSSGGG--------------------- 1320
            DWEKS SDRGGFGG GRGR              WSSGGG                     
Sbjct: 1261 DWEKSSSDRGGFGGRGRGRWNRERGSNDGENRGWSSGGGDRERSGSDRGGFRGRGRGRWN 1320

Query: 1321 -------------------------------------GSDSG------WGKSGSDRGGFG 1380
                                                 G DSG      W KS SDRGGF 
Sbjct: 1321 QEGGSRDGDNGGGWSGGRGGYGGRGRGRWNQEGGSNDGGDSGGRGGGSWEKSQSDRGGF- 1380

Query: 1381 GGRGRGRWN-QGDSNSNDGGNGGWSSSGG-------RGGFGGGRGRGRWNQEGDSSSKDS 1440
            GGRGRGRWN +GD+N  D G GGWSS G        RGGFGGG G G+W+QEG   S +S
Sbjct: 1381 GGRGRGRWNREGDTNDGDNG-GGWSSGGNWGKSGSDRGGFGGG-GSGKWSQEG--GSNNS 1440

Query: 1441 DNGGWSGGGGG-WGSSGSGRGGIGGRGRGRRNQDGGSN--DNEDKPASWSTGSGNTGGWS 1500
            D GGWSGGGGG WG SGS RGG GGRGRGRRNQDGGSN  DN DKPASWSTGSGN GGWS
Sbjct: 1441 DYGGWSGGGGGSWGKSGSDRGGFGGRGRGRRNQDGGSNDDDNVDKPASWSTGSGNNGGWS 1500

Query: 1501 SSGGAGSWSRGGDEKNQQQSWKTNNEGGDQSGGQGSGWKEPSGNEQNTWKSSGA------ 1521
            S GGAGSW+ GGD+KNQQQSWK++N+      G+GSGWK PSGNE   WK+SG+      
Sbjct: 1501 SGGGAGSWNGGGDDKNQQQSWKSSND-----AGEGSGWKAPSGNENINWKASGSSGVDDN 1560

BLAST of MS010097 vs. NCBI nr
Match: XP_022957824.1 (protein RNA-directed DNA methylation 3-like [Cucurbita moschata])

HSP 1 Score: 1900.6 bits (4922), Expect = 0.0e+00
Identity = 1147/1652 (69.43%), Postives = 1250/1652 (75.67%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKDTS+GKRK R+D + +  ARKRRDRSVLQFFEDV+PE+G  SDDSDFLDD
Sbjct: 1    MASKGKGIAKDTSAGKRKLRDDTD-SSAARKRRDRSVLQFFEDVSPELGAYSDDSDFLDD 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEEEF+  PAFKND +K QNIPFFPKEEEMNEEEFDR+MEEHY +GPGLGAFAEENYEN
Sbjct: 61   FMEEEFEPVPAFKNDDSKAQNIPFFPKEEEMNEEEFDRMMEEHYTRGPGLGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            K STGRNP Q S+RD ISLWKVKCMVG ERQSVFCLMQKFVDLHSFGTKLQIKSAF V+H
Sbjct: 121  KISTGRNPPQQSARDNISLWKVKCMVGHERQSVFCLMQKFVDLHSFGTKLQIKSAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            +KGFIYIEAPRQYDLIEACKGI+GIYSTRIASVPENDISQLLTV+SRVSEV+VGTMARVK
Sbjct: 181  IKGFIYIEAPRQYDLIEACKGINGIYSTRIASVPENDISQLLTVQSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKL+PRIDLQ+MA KFGGGV AKK TNPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLIPRIDLQSMAEKFGGGVVAKKATNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            +EFRPLMQFRRDRETGKLFEF DGMMLKDGYL+KK+SLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  EEFRPLMQFRRDRETGKLFEFFDGMMLKDGYLYKKVSLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNE+NDLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSSM SF DHDLVCFGRKDFGMI
Sbjct: 361  SNEANDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSMISFGDHDLVCFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LGTEKDDSYKILK+GPDGS+VVNVQRKELKSGPL+ KFT+ D NGKIISVSDNVKVLEGS
Sbjct: 421  LGTEKDDSYKILKDGPDGSVVVNVQRKELKSGPLDAKFTSVDLNGKIISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSN+C KIKISYDAPSGK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNLCAKIKISYDAPSGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSP+KPWAEK+ GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAV K
Sbjct: 541  DFSSSPKSPLSPQKPWAEKE-GREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVHK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTV---EDTEFGSLKPFDILGNEGSSQDWMGG 660
            +DVTVKLDSQQKVLTVRSD L+EV+RK+S V   ED EF SLKPFDILGNEGSSQDWMGG
Sbjct: 601  RDVTVKLDSQQKVLTVRSDLLAEVHRKSSAVSVSEDPEFSSLKPFDILGNEGSSQDWMGG 660

Query: 661  TGSSTGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIIT--DVEDSP 720
            TGSS   DGWNSAGPSSERSPWPSFPESGT      P SS ++ + +  +    DVEDSP
Sbjct: 661  TGSSAAADGWNSAGPSSERSPWPSFPESGT---LNCPGSSSTNPFGSENLDAKKDVEDSP 720

Query: 721  WVSKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVV 780
            WVSK+   A+TSWGAA S+V DT NDDQA GWGKSESWDKATAKT SDGN SGAW K VV
Sbjct: 721  WVSKSTAEANTSWGAANSSV-DTANDDQACGWGKSESWDKATAKTISDGNVSGAWGKSVV 780

Query: 781  PGGDY------------------------------------KDSESGGWKKSQNASFGDE 840
            P GD                                     KDSESGGWKK+ + S GD 
Sbjct: 781  PSGDSAGDKWDKGKHVTSDNQTGKWGGGTSGKNELSAWSRDKDSESGGWKKNPSVSVGDN 840

Query: 841  NNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQKQ 900
            N  AE SA+KW SKNRS+G WGD N STTVSEI+ AGK N G                  
Sbjct: 841  NTPAETSADKWGSKNRSNGSWGDQNVSTTVSEIQTAGKDNVG------------------ 900

Query: 901  ESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKPN 960
                  W KP                    G+  + + S WN+ T+  G G+   W    
Sbjct: 901  -----GWTKP--------------------GAESKINPSGWNEDTSMKG-GQTSNW---- 960

Query: 961  LASEDESIGKKGWGQGNEASDSGNK-WQSSGS--DGGKKW-GTNESERDGGNKSWNTSKS 1020
              + DE+    GWG+     D GN  W  S +  DG   W  +NESER+GGN+SWN SKS
Sbjct: 961  -GNRDEA---GGWGKPMNVGDGGNSAWNKSTACGDGNDSWKKSNESEREGGNRSWNASKS 1020

Query: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080
            SDGD     SAIWK+KSDSSSLTA KGDQWGG W+KQHSSN+TKASEDNSPWNKKSVESG
Sbjct: 1021 SDGD-----SAIWKEKSDSSSLTASKGDQWGGGWNKQHSSNETKASEDNSPWNKKSVESG 1080

Query: 1081 KDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS-- 1140
            KD E +++GSGWNVGK+S GDSASGWGQTS+EAGSSDQ  SWGSNWKK S+ GNEDSS  
Sbjct: 1081 KDNELENQGSGWNVGKTS-GDSASGWGQTSREAGSSDQGSSWGSNWKKKSDTGNEDSSSA 1140

Query: 1141 -------------WAEKSNWNSGNESNDNQFT----GDTSGHGSWGGDGSDRGGFRGRGG 1200
                         W EKSNWNSGNE N N         T     W G+ SDRGGFRGRG 
Sbjct: 1141 KKSNWSSGSGNSNWGEKSNWNSGNEFNANHSNDGAEAQTEVSNDWRGESSDRGGFRGRGS 1200

Query: 1201 FRGRGERGRFGGRGRSD-----RGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG 1260
            FRGRGERGRFGGRGRSD     RGGSDRGGFGGRGRGRWNSEGGSNDG+N+GWSS  GGG
Sbjct: 1201 FRGRGERGRFGGRGRSDRGGFGRGGSDRGGFGGRGRGRWNSEGGSNDGDNKGWSS--GGG 1260

Query: 1261 DWEKSGSDRGGFGG-GRGRWSSGGGGSDS---GWG-------KSGSDRGGFGGGRGRGRW 1320
            DWEKS SDRGGFGG GRGRW+   G +D    GW        +SGSDRGGF  GRGRGRW
Sbjct: 1261 DWEKSSSDRGGFGGRGRGRWNRESGSNDGENRGWSSGGGDRERSGSDRGGF-RGRGRGRW 1320

Query: 1321 NQGDSNSNDGGN-GGWSSSGGRGGFGGGRGRGRWNQEGDSS------------------- 1380
            NQ +  S DG N GGW  SGGRGG+ GGRGRGRWNQEG S+                   
Sbjct: 1321 NQ-EGGSRDGDNGGGW--SGGRGGY-GGRGRGRWNQEGGSNDGGDSVGWGGGGNWGKSGS 1380

Query: 1381 ------------------SKDSDNGGWSGGGGG-WGSSGSGRGGIGGRGRGRRNQDGG-- 1440
                              S +SD GGWSGGGGG WG SGS RGG GGRGRGRRNQDGG  
Sbjct: 1381 DRGGFGGGGSGKWSQEGGSNNSDYGGWSGGGGGSWGKSGSDRGGFGGRGRGRRNQDGGLK 1440

Query: 1441 SNDNEDKPASWSTGSGNTGGWSSSGG-AGSWSRGGDEKNQQQSWKTNNEGGDQSGGQGSG 1500
             +DN DKPASWSTGSGN GGW S GG AGSW+ GGD+KNQQQSWK++N+      G+GSG
Sbjct: 1441 DDDNVDKPASWSTGSGNNGGWGSGGGTAGSWNGGGDDKNQQQSWKSSND-----AGEGSG 1500

Query: 1501 WKEPSGNEQNTWKSSGA------SGWNKSSAGKDIEESCSQKSSWGQAAAKTDAQAGGGW 1521
            WK PSGNE   WK+SG+      SGWNKS+A K+IEES  QK+S G+AA       GGGW
Sbjct: 1501 WKAPSGNENINWKASGSSGVDDNSGWNKSTACKEIEESGDQKNSRGKAAKADIHIQGGGW 1560

BLAST of MS010097 vs. NCBI nr
Match: XP_022992957.1 (protein RNA-directed DNA methylation 3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 1899.0 bits (4918), Expect = 0.0e+00
Identity = 1144/1681 (68.05%), Postives = 1234/1681 (73.41%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKD S GKRK R+DNN +  ARKRRDRSVLQFFEDVAPE GGESDDSDF D 
Sbjct: 1    MASKGKGIAKDASPGKRKLRDDNN-SSAARKRRDRSVLQFFEDVAPEFGGESDDSDFFDG 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEE+F+T PAFKND AK QNIPFFPKEEEMNEEEFDR+MEEHYNQGPG GAFAEENYEN
Sbjct: 61   FMEEDFETIPAFKNDDAKSQNIPFFPKEEEMNEEEFDRMMEEHYNQGPGFGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNP   S+RDTISLWKVKCMVGRERQSVFCLMQKFVDL+SFGTKLQIK+AF V+H
Sbjct: 121  KNSTGRNPPLQSARDTISLWKVKCMVGRERQSVFCLMQKFVDLNSFGTKLQIKAAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTR+ASVPENDISQLLTVRSRVSEV+VGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRVASVPENDISQLLTVRSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMA KFGGG AAKKT+NPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAEKFGGGSAAKKTSNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNES+DLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSS +SF DHDL+CFGRKDFGMI
Sbjct: 361  SNESHDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSTSSFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LG EKDDSYKILK+ P+GS+VVNVQRKELK G L+ KFTAADHNGK+ISVSDNVKVLEGS
Sbjct: 421  LGMEKDDSYKILKDSPNGSVVVNVQRKELKGGSLDAKFTAADHNGKMISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEV+NDGYFCCKSN CEKIKISYDAP GK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVENDGYFCCKSNKCEKIKISYDAPCGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK++GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKETGREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTVEDTEFGSLKPFDILGNEGSSQDWMGGTGS 660
            +DVTVKLDSQQKVLTVRSDFLSEV RK+S V  +E   LKPFDILGNEGSSQDWMGGTGS
Sbjct: 601  RDVTVKLDSQQKVLTVRSDFLSEVQRKSSAVSLSE-DPLKPFDILGNEGSSQDWMGGTGS 660

Query: 661  STGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKT 720
            S GGDGWNS GPSSE +PWPSFPES T       SSS +     S      EDSPWVSK+
Sbjct: 661  SAGGDGWNSTGPSSEGNPWPSFPESSTLNG--PGSSSTNPIGSESFDANKDEDSPWVSKS 720

Query: 721  VPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDY 780
             P+ASTSWGAAKS+V DT N+ QA GWGKS+SW K  AKT SDGNASGAW K  VP GD 
Sbjct: 721  TPDASTSWGAAKSSV-DTANNGQASGWGKSDSWGKTIAKTCSDGNASGAWGKTAVPSGDS 780

Query: 781  ------------------------------------------KDSESGGWKKSQNASFGD 840
                                                      KD+ESGGWKK+Q+A+F D
Sbjct: 781  AGLTENTWDKWDKGKQVSSDNQTGNWDNGTSGKNEHSAWSRDKDAESGGWKKTQSANFDD 840

Query: 841  ENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQK 900
            +   AE           S+G W             P  ++    S WN         T  
Sbjct: 841  DKTPAE-----------SAGDW-----------TNPEAENKVNPSGWN-------EGTSM 900

Query: 901  QESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKP 960
            + S  S+WG   + GG      G+  N   GG      SS WNKST+ DGA + D WNKP
Sbjct: 901  KGSQTSNWGNQDETGGW-----GKPKNVGNGG------SSAWNKSTSGDGAVENDSWNKP 960

Query: 961  NLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKSSDG 1020
             L S DESIGKKGWGQ NEASD+GNKWQSS SDGG KWGTNESE +GG            
Sbjct: 961  KLFSHDESIGKKGWGQSNEASDNGNKWQSSRSDGGTKWGTNESEHEGG------------ 1020

Query: 1021 DTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESGKDG 1080
                      KDKSDSSSLT P+GDQ  G WDKQ SSNDTKASE+NSPWNKKSVESGKDG
Sbjct: 1021 ----------KDKSDSSSLTTPRGDQSVGGWDKQRSSNDTKASEENSPWNKKSVESGKDG 1080

Query: 1081 ERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS----- 1140
            E K++GSGWNVGK+SGGDSASGWGQ SKEAGSSD  G+WGSNWKKNS+ GNEDSS     
Sbjct: 1081 ELKNQGSGWNVGKTSGGDSASGWGQASKEAGSSDLVGNWGSNWKKNSDVGNEDSSLAKKS 1140

Query: 1141 ----------WAEKSNWNSGNESNDNQFTG----------DTSGHGSWGGDGSDRGGFRG 1200
                      W EKSNWNSGNE N N  TG          DTSG+GSW G+ SDRGG+RG
Sbjct: 1141 NWSSGSGNSNWGEKSNWNSGNEYNANHSTGGAEAQTDVSNDTSGYGSWRGENSDRGGYRG 1200

Query: 1201 RGGFRGRGERGRFGGRGRSDRGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG-- 1260
            RGGFRGRGERGRFGGRGRSDRGG  RGGFGGRGRGRWNSEGGSN G+N+GWSSGGGGG  
Sbjct: 1201 RGGFRGRGERGRFGGRGRSDRGGFGRGGFGGRGRGRWNSEGGSNGGDNKGWSSGGGGGDN 1260

Query: 1261 ------------------------------------------------------------ 1320
                                                                        
Sbjct: 1261 KGWSSGGGGGDNRGWSSGGGGDDNKGWSSGGAGDNKGWGGGGGGDNKGWSSGGGDNKGWS 1320

Query: 1321 --------DWEKSGSDRGGFGG-GRGRWSSGGGGSDSGWGKSGSDRGGFGGGRGRGRWNQ 1380
                    DWEKSGSDRGGFGG GRGRWSSG G  D    K GSDRGGF  GRGRGRWNQ
Sbjct: 1321 GGGGGGSSDWEKSGSDRGGFGGRGRGRWSSGSGSHDGDREKFGSDRGGF-RGRGRGRWNQ 1380

Query: 1381 GDSNSNDGGNGGWSSSGGRGGFGGGRGRGRWNQEGDSSSKDSDNGGW-SGGGGGWGSSGS 1440
             D  S+DG NGGW   GGRGG+ GGRGRGRWNQEGDS+  D+  GGW SG GG WG SGS
Sbjct: 1381 -DGGSHDGDNGGW--RGGRGGY-GGRGRGRWNQEGDSNDGDNGGGGWSSGNGGSWGKSGS 1440

Query: 1441 G-RGGIGGRGRGRRNQDGGSNDNEDKPASWSTGSGNTGGWSSSGGAGSWSRGGDEKNQQQ 1500
               GG GGRG G+ NQ+GGSN+N              GGWSS GGAGSW++GGDEKNQ Q
Sbjct: 1441 DIGGGFGGRGSGKWNQEGGSNNN--------------GGWSSGGGAGSWNQGGDEKNQAQ 1500

Query: 1501 SWKTNNEGGDQSGG-QGSGWKEP-SGNEQNTWKSSGA------SGWNKSSAGKDIEESCS 1521
             WK +N+GGD+ GG QGSGWKEP SGNE N WKSSG+      SGWNKS+ GK++EES S
Sbjct: 1501 GWKPSNDGGDKFGGQQGSGWKEPTSGNEPNNWKSSGSSGADHNSGWNKSTTGKEMEESDS 1560

BLAST of MS010097 vs. NCBI nr
Match: XP_022992954.1 (protein RNA-directed DNA methylation 3-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1899.0 bits (4918), Expect = 0.0e+00
Identity = 1144/1681 (68.05%), Postives = 1234/1681 (73.41%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKD S GKRK R+DNN +  ARKRRDRSVLQFFEDVAPE GGESDDSDF D 
Sbjct: 1    MASKGKGIAKDASPGKRKLRDDNN-SSAARKRRDRSVLQFFEDVAPEFGGESDDSDFFDG 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEE+F+T PAFKND AK QNIPFFPKEEEMNEEEFDR+MEEHYNQGPG GAFAEENYEN
Sbjct: 61   FMEEDFETIPAFKNDDAKSQNIPFFPKEEEMNEEEFDRMMEEHYNQGPGFGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNP   S+RDTISLWKVKCMVGRERQSVFCLMQKFVDL+SFGTKLQIK+AF V+H
Sbjct: 121  KNSTGRNPPLQSARDTISLWKVKCMVGRERQSVFCLMQKFVDLNSFGTKLQIKAAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTR+ASVPENDISQLLTVRSRVSEV+VGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRVASVPENDISQLLTVRSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMA KFGGG AAKKT+NPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAEKFGGGSAAKKTSNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNES+DLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSS +SF DHDL+CFGRKDFGMI
Sbjct: 361  SNESHDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSTSSFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LG EKDDSYKILK+ P+GS+VVNVQRKELK G L+ KFTAADHNGK+ISVSDNVKVLEGS
Sbjct: 421  LGMEKDDSYKILKDSPNGSVVVNVQRKELKGGSLDAKFTAADHNGKMISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEV+NDGYFCCKSN CEKIKISYDAP GK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVENDGYFCCKSNKCEKIKISYDAPCGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK++GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKETGREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTVEDTEFGSLKPFDILGNEGSSQDWMGGTGS 660
            +DVTVKLDSQQKVLTVRSDFLSEV RK+S V  +E   LKPFDILGNEGSSQDWMGGTGS
Sbjct: 601  RDVTVKLDSQQKVLTVRSDFLSEVQRKSSAVSLSE-DPLKPFDILGNEGSSQDWMGGTGS 660

Query: 661  STGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKT 720
            S GGDGWNS GPSSE +PWPSFPES T       SSS +     S      EDSPWVSK+
Sbjct: 661  SAGGDGWNSTGPSSEGNPWPSFPESSTLNG--PGSSSTNPIGSESFDANKDEDSPWVSKS 720

Query: 721  VPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDY 780
             P+ASTSWGAAKS+V DT N+ QA GWGKS+SW K  AKT SDGNASGAW K  VP GD 
Sbjct: 721  TPDASTSWGAAKSSV-DTANNGQASGWGKSDSWGKTIAKTCSDGNASGAWGKTAVPSGDS 780

Query: 781  ------------------------------------------KDSESGGWKKSQNASFGD 840
                                                      KD+ESGGWKK+Q+A+F D
Sbjct: 781  AGLTENTWDKWDKGKQVSSDNQTGNWDNGTSGKNEHSAWSRDKDAESGGWKKTQSANFDD 840

Query: 841  ENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQK 900
            +   AE           S+G W             P  ++    S WN         T  
Sbjct: 841  DKTPAE-----------SAGDW-----------TNPEAENKVNPSGWN-------EGTSM 900

Query: 901  QESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKP 960
            + S  S+WG   + GG      G+  N   GG      SS WNKST+ DGA + D WNKP
Sbjct: 901  KGSQTSNWGNQDETGGW-----GKPKNVGNGG------SSAWNKSTSGDGAVENDSWNKP 960

Query: 961  NLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKSSDG 1020
             L S DESIGKKGWGQ NEASD+GNKWQSS SDGG KWGTNESE +GG            
Sbjct: 961  KLFSHDESIGKKGWGQSNEASDNGNKWQSSRSDGGTKWGTNESEHEGG------------ 1020

Query: 1021 DTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESGKDG 1080
                      KDKSDSSSLT P+GDQ  G WDKQ SSNDTKASE+NSPWNKKSVESGKDG
Sbjct: 1021 ----------KDKSDSSSLTTPRGDQSVGGWDKQRSSNDTKASEENSPWNKKSVESGKDG 1080

Query: 1081 ERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS----- 1140
            E K++GSGWNVGK+SGGDSASGWGQ SKEAGSSD  G+WGSNWKKNS+ GNEDSS     
Sbjct: 1081 ELKNQGSGWNVGKTSGGDSASGWGQASKEAGSSDLVGNWGSNWKKNSDVGNEDSSLAKKS 1140

Query: 1141 ----------WAEKSNWNSGNESNDNQFTG----------DTSGHGSWGGDGSDRGGFRG 1200
                      W EKSNWNSGNE N N  TG          DTSG+GSW G+ SDRGG+RG
Sbjct: 1141 NWSSGSGNSNWGEKSNWNSGNEYNANHSTGGAEAQTDVSNDTSGYGSWRGENSDRGGYRG 1200

Query: 1201 RGGFRGRGERGRFGGRGRSDRGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG-- 1260
            RGGFRGRGERGRFGGRGRSDRGG  RGGFGGRGRGRWNSEGGSN G+N+GWSSGGGGG  
Sbjct: 1201 RGGFRGRGERGRFGGRGRSDRGGFGRGGFGGRGRGRWNSEGGSNGGDNKGWSSGGGGGDN 1260

Query: 1261 ------------------------------------------------------------ 1320
                                                                        
Sbjct: 1261 KGWSSGGGGGDNRGWSSGGGGDDNKGWSSGGAGDNKGWGGGGGGDNKGWSSGGGDNKGWS 1320

Query: 1321 --------DWEKSGSDRGGFGG-GRGRWSSGGGGSDSGWGKSGSDRGGFGGGRGRGRWNQ 1380
                    DWEKSGSDRGGFGG GRGRWSSG G  D    K GSDRGGF  GRGRGRWNQ
Sbjct: 1321 GGGGGGSSDWEKSGSDRGGFGGRGRGRWSSGSGSHDGDREKFGSDRGGF-RGRGRGRWNQ 1380

Query: 1381 GDSNSNDGGNGGWSSSGGRGGFGGGRGRGRWNQEGDSSSKDSDNGGW-SGGGGGWGSSGS 1440
             D  S+DG NGGW   GGRGG+ GGRGRGRWNQEGDS+  D+  GGW SG GG WG SGS
Sbjct: 1381 -DGGSHDGDNGGW--RGGRGGY-GGRGRGRWNQEGDSNDGDNGGGGWSSGNGGSWGKSGS 1440

Query: 1441 G-RGGIGGRGRGRRNQDGGSNDNEDKPASWSTGSGNTGGWSSSGGAGSWSRGGDEKNQQQ 1500
               GG GGRG G+ NQ+GGSN+N              GGWSS GGAGSW++GGDEKNQ Q
Sbjct: 1441 DIGGGFGGRGSGKWNQEGGSNNN--------------GGWSSGGGAGSWNQGGDEKNQAQ 1500

Query: 1501 SWKTNNEGGDQSGG-QGSGWKEP-SGNEQNTWKSSGA------SGWNKSSAGKDIEESCS 1521
             WK +N+GGD+ GG QGSGWKEP SGNE N WKSSG+      SGWNKS+ GK++EES S
Sbjct: 1501 GWKPSNDGGDKFGGQQGSGWKEPTSGNEPNNWKSSGSSGADHNSGWNKSTTGKEMEESDS 1560

BLAST of MS010097 vs. ExPASy Swiss-Prot
Match: F4JW79 (Protein RNA-directed DNA methylation 3 OS=Arabidopsis thaliana OX=3702 GN=RDM3 PE=1 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 4.7e-187
Identity = 630/1634 (38.56%), Postives = 842/1634 (51.53%), Query Frame = 0

Query: 1    MASKGKG---IAKDTSSGKRKQREDNNGAD---LARKRRDRSVLQFFEDVAP--EVGGES 60
            M  KGKG      D+ SG +K++      D     +KR++  VLQFFE+ A     GG S
Sbjct: 1    MDRKGKGKQVAGSDSYSGGQKRKNSVEFRDEGLRIKKRKNPEVLQFFEESAEVGYYGGSS 60

Query: 61   DDSD----FLDDFMEEEFDTEPAFK-NDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQG 120
            D+ D    FL+D ME+E + E + K     K ++   FPKEE++NEEEFDRIMEE Y  G
Sbjct: 61   DEDDDGLGFLND-MEDEPEVEESSKAGKGEKGKSSFVFPKEEDLNEEEFDRIMEERYKPG 120

Query: 121  PGLGAFAEENYENKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFG 180
             G   +A+++   K++   + L P+S+D   +WKVKC +GRER+SVFCLM KFV+L   G
Sbjct: 121  SGFLRYADDDI--KDAIEMDALAPTSKDP-PIWKVKCAIGRERRSVFCLMHKFVELRKIG 180

Query: 181  TKLQIKSAFYVDHVKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSR 240
            TKL+I S F VDHVKGFI+IEA +++D++EACK + GIY+TR+  +P+ +   LLTV+ +
Sbjct: 181  TKLEIISVFSVDHVKGFIFIEADKEHDVLEACKSLVGIYATRMVLLPKAETPNLLTVQKK 240

Query: 241  VSEVSVGTMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKT 300
              +VS GT ARVKNGKYKGDLAQIVAV++ R +A +KL+PRID+QA+  K+GGGV  +K 
Sbjct: 241  TKKVSEGTWARVKNGKYKGDLAQIVAVSDTRNKALIKLIPRIDIQALTQKYGGGVTVQKG 300

Query: 301  TNPAPRLINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVM 360
              PAPRLI+SSEL+EFRPL+Q RRDR+TG  FE LD +MLKDGYL+KK+SLDS++ WGV+
Sbjct: 301  QTPAPRLISSSELEEFRPLIQVRRDRDTGITFEHLDSLMLKDGYLYKKVSLDSISSWGVI 360

Query: 361  PSEDELLKFKPPESNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGS--------- 420
            P++DELLKF P +  E+ D+EW+S++YGE+RKKKI+ T + GGKGEG+ G          
Sbjct: 361  PTKDELLKFTPVDRKETGDVEWISEIYGEERKKKILPTCREGGKGEGSGGGKGEGSGGGK 420

Query: 421  ---------------SSMNSFADHDLVCFGRKDFGMILGT-EKDDSYKILKEGPDGSIVV 480
                            S +S+  ++LVCF RKDFG+I+G  +K D YK+LKEG DG +VV
Sbjct: 421  GEGSRGGKGEGSSDFKSESSYELYNLVCFSRKDFGLIVGVDDKGDGYKVLKEGIDGPVVV 480

Query: 481  NVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVFVYDEN 540
             V +KE+++GP + KFTA D N K ISV+D VK+ +G  + KQG+V+ VYR  +F+YDE+
Sbjct: 481  TVGKKEMQNGPFDSKFTALDLNKKQISVNDVVKISKGPSEGKQGVVRQVYRGIIFLYDES 540

Query: 541  EVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWAEKDSG 600
            E +N GYFCCKS  CEK+K+  +  + K      + FEDF SSPKSPLSP+K W  ++  
Sbjct: 541  EEENGGYFCCKSQSCEKVKLFTEESNEKTGGFDGTAFEDFVSSPKSPLSPEKEWQPRERY 600

Query: 601  REYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKKDVTVKLDSQQKVLTVRSDFLSE 660
               N+ D    +SIGQ LRIRVGPLKGYLCRVIA+R  DVTVKLDSQ K+ TV+S+ L+E
Sbjct: 601  NSSNQGDIGSTYSIGQKLRIRVGPLKGYLCRVIALRYSDVTVKLDSQHKIFTVKSEHLAE 660

Query: 661  VYRKT---STVEDTEFGSLKPFDILGNEGSSQDWMGGTGSSTGGDGWNSAGPSSERSPWP 720
            V  +    ST  D   GS +PF +LG E S+ DW  G G+S+ G  WN  GPS++     
Sbjct: 661  VRDRNTVLSTSGDAGTGSFQPFGMLGTESSTGDWAIGAGTSSEGGNWNIGGPSTD----- 720

Query: 721  SFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKTVPNASTSWGAAK--SNVDDT 780
                           S  S     +M+    E +P            WG +K  S+V  T
Sbjct: 721  ---------------SHESLNIERNMVQLCREKNP------------WGGSKPTSDVSPT 780

Query: 781  VNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDYKDSESG---GWKKSQNAS 840
            V DD    W  +     A  K +S  +  G W+    P G    SE+G   GW       
Sbjct: 781  VADDNTSAWANA----AAENKPASASDQPGGWN----PWGKTPASEAGTVSGW------- 840

Query: 841  FGDENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSW- 900
               + +++   A+ W  +  S+    D  +  T          + GSS  N  DE+S W 
Sbjct: 841  --GDTSASNVEASSWEKQGASTSNVADLGSWGT----------HGGSSGGNKQDEDSVWG 900

Query: 901  -----NTQKQESNRSSWGKPSDRGGTRSSESGRGGN----QVRGGSFG-QDSSSD----- 960
                 +   Q+   SSWGK     G  SS   + GN    +  G S+G QD  SD     
Sbjct: 901  KLCEASESSQKKEESSWGKKGGSDG-ESSWGNKDGNSSASKKDGVSWGQQDKGSDESKGG 960

Query: 961  --WNKSTTTDGAGKK----DGWNKPNLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGG 1020
              W+      G+GKK     GWNK    S   S G   WGQ N+ S  G K      DG 
Sbjct: 961  SAWSNQCGDFGSGKKKDGSSGWNKSAEDSNANSKGVPDWGQPNDGSSWGKK-----GDGA 1020

Query: 1021 KKWGTNESERDGGNKSWNTSKSSDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQH 1080
              WG  +   DGG  SW   K  DG+   G S+ W  K D         D  G  W+K+ 
Sbjct: 1021 ASWGKKD---DGG--SW--GKKDDGNKDDGGSS-WGKKDDGQK------DDGGSSWEKKF 1080

Query: 1081 SSNDT--KASEDNSPWNKKSVESGKDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSS 1140
                +  K  +  S W KK       G++ D GS W  GK   G S   WG+  K+ G S
Sbjct: 1081 DGGSSWGKKDDGGSSWGKKDDGGSLWGKKDDGGSSW--GKEDDGGSL--WGK--KDDGES 1140

Query: 1141 DQAGSWGSNWKKNSNAGNEDSSWAEK----SNWNSGNESNDNQFTGDTSGHGSWGGDGSD 1200
                SWG   KK+      +SSW +K    S+W   +E   ++ T D  G G  G  G  
Sbjct: 1141 ----SWG---KKDDG----ESSWGKKDDGGSSWGKKDEGGYSEQTFDRGGRGFGGRRGGG 1200

Query: 1201 RGGFR---GRGGFRGRGE---------RGRFGGRGRSDRGGS------DRGG---FGGRG 1260
            R G R   GRG   G  E          G   G+   D GGS      D GG   +G + 
Sbjct: 1201 RRGGRDQFGRGSSFGNSEDPAPWSKPSGGSSWGKQDGDGGGSSWGKENDAGGGSSWGKQD 1260

Query: 1261 RGRWNSEGGSNDGENRGWSSG-----GGGGDWEKSGSDRGGFGGGRGRWSSGGGGSDSGW 1320
             G  +S G  NDG   G S G     GGG  W K   D GG G   G+   GGG S S W
Sbjct: 1261 NGVGSSWGKQNDGSGGGSSWGKQNDAGGGSSWGK--QDSGGDGSSWGK-QDGGGDSGSAW 1320

Query: 1321 GKSGSDRGGFGGGRGRGRWNQGDSNSNDGGNGG--WSSSGGRGGFGGGRGRGRWNQEGDS 1380
            GK  +  GG   G+             DGG GG  W    G GG G   G+      G S
Sbjct: 1321 GKQNNTSGGSSWGKQSDAGGGSSWGKQDGGGGGSSWGKQDGGGGSGSAWGKQNETSNGSS 1380

Query: 1381 SSKDSDNGGWSGGGGGWGSSGSGRGGIGGRGRGRRNQDGGSN------DNEDKPASWSTG 1440
              K +D    SGGG  WG      GG GG   G++N  GG +      D   KP  W+  
Sbjct: 1381 WGKQND----SGGGSSWGKQD---GGGGGSSWGKQNDGGGGSSWGKQGDGGSKP--WNEH 1440

Query: 1441 SGNTGGWSSSGGAGSWSRGGDEKNQQQSWKTNNEGGDQSGGQGSGWKEPSGNEQNTWKS- 1500
            SG   G+    G G + RGG  +NQ     +   G    GG+ S WK  + N++NTWKS 
Sbjct: 1441 SGGGRGFGERRGGGGF-RGG--RNQ-----SGRGGRSFDGGRSSSWK--TDNQENTWKSD 1485

Query: 1501 -SGASGWNKSSAGKDIEESCSQKSSWGQAA--AKTDAQAGGGWGKSASGWNGGSEDQTTK 1520
             SG S W               K  WG+ +  +K    + GG   +   W+  S+ +T  
Sbjct: 1501 QSGGSDW---------------KKGWGEDSNNSKPSGSSAGGCAGNWPSWDTNSKKETND 1485

BLAST of MS010097 vs. ExPASy Swiss-Prot
Match: Q9STN3 (Putative transcription elongation factor SPT5 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=At4g08350 PE=1 SV=2)

HSP 1 Score: 275.0 bits (702), Expect = 5.0e-72
Identity = 222/733 (30.29%), Postives = 346/733 (47.20%), Query Frame = 0

Query: 4   KGKGIAKDTSSGKRKQREDNN---------GADLARKRRDRSVLQFFEDVAPEVG--GES 63
           +G+    D  + +  Q ED++         G   A KR+  S   F +  A +V    E 
Sbjct: 45  RGRSNFIDDYAEEDSQEEDDDDEDYGSSRGGKGAASKRKKPSASIFLDREAHQVDDEDEE 104

Query: 64  DDSDFLDDFMEEEFDTEPAFKNDAAKDQNIPFFPKEE-EMNEEEFDRIMEEHYNQGPGLG 123
           ++ +  DDF+ +     P  + D   ++   F P++E + + E+ +R ++E ++      
Sbjct: 105 EEDEAEDDFIVDNGTDLPDERGDRRYERR--FLPRDENDEDVEDLERRIQERFS-----S 164

Query: 124 AFAEENYENKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQ 183
              EE  E      +  L PS RD   LW VKC +GRER+   CLMQKF+D    G  LQ
Sbjct: 165 RHHEEYDEEATEVEQQALLPSVRDP-KLWMVKCAIGREREVAVCLMQKFIDR---GADLQ 224

Query: 184 IKSAFYVDHVKGFIYIEAPRQYDLIEACKGISGIYST-RIASVPENDISQLLTVRSRVSE 243
           I+S   +DH+K FIY+EA ++  + EA KG+  IY+  +I  VP  +++ +L+V S+  +
Sbjct: 225 IRSVVALDHLKNFIYVEADKEAHVKEAIKGMRNIYANQKILLVPIREMTDVLSVESKAID 284

Query: 244 VSVGTMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGG-GVAAKKTTN 303
           +S  T  R+K G YKGDLA++V V+N R+R TVKL+PRIDLQA+A+K  G  V+ KK   
Sbjct: 285 LSRDTWVRMKIGTYKGDLAKVVDVDNVRQRVTVKLIPRIDLQALASKLDGREVSKKKAFV 344

Query: 304 PAPRLINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPS 363
           P PR +N  E  E    ++ RRD  TG  FE + GM+ KDG+ +K++SL S+    V P+
Sbjct: 345 PPPRFMNIDEARELHIRVERRRDHMTGDYFENIGGMLFKDGFHYKQVSLKSITVQNVTPT 404

Query: 364 EDELLKFKPPESNESNDLEWLSQLYGEKRK-----------------------------K 423
            DEL KF  P  N   D   LS L+  ++K                              
Sbjct: 405 FDELEKFNKPSENGEGDFGGLSTLFANRKKGHFMKGDAVIVIKGDLKNLKGWVEKVDEEN 464

Query: 424 KIIRTEKGG--------------------------GKGEG-------------------- 483
            +IR+E  G                          G  EG                    
Sbjct: 465 VLIRSEVKGLPDPLAVNERELCKYFEPGNHVKVVSGTHEGATGMVVKVDQHVLIILSDTT 524

Query: 484 -----------------TSGSSSMNSFADHDLVCFGRKDFGMILGTEKDDSYKILKEGPD 543
                            T+G + +  +  HDLV      FG+I+  E ++++++LK  PD
Sbjct: 525 KEHVRVFADHVVESSEVTTGVTKIGDYELHDLVLLDNLSFGVIIRLE-NEAFQVLKGVPD 584

Query: 544 GSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVF 603
              V  V+ +E+K   LE K    D    +I+V D+V+V+EG  K KQG VKH+Y+  +F
Sbjct: 585 RPEVALVKLREIKC-KLEKKINVQDRYKNVIAVKDDVRVIEGPSKGKQGPVKHIYKGVLF 644

Query: 604 VYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWA 623
           +YD + +++ G+ C K   C  +  S    +    D   S + +F +    P SP +   
Sbjct: 645 IYDRHHLEHAGFICAKCTSCIVVGGSRSGANRNGGD-SLSRYGNFKAPAPVPSSPGR--F 704

BLAST of MS010097 vs. ExPASy Swiss-Prot
Match: O80770 (Putative transcription elongation factor SPT5 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=At2g34210 PE=3 SV=2)

HSP 1 Score: 241.9 bits (616), Expect = 4.7e-62
Identity = 217/739 (29.36%), Postives = 336/739 (45.47%), Query Frame = 0

Query: 14  SGKRKQR--EDNNGADLARKRRDRSVLQFFE-DVAPEVGGESDDSDFLD--------DF- 73
           SGK++ R   D++G   ++K+   S    +E +V  +V  + DD D  D        DF 
Sbjct: 36  SGKKRGRSNSDSDGRRGSKKKSSGSAFIDWEVEVDDDVEDDDDDVDVEDGKQQLKFGDFS 95

Query: 74  ----MEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEEN 133
               +  E D      +   +     F P EE+++E E  R +E    +      +A+++
Sbjct: 96  LCFIVSGEADLPNEDSDHRRQYYQRGFHPHEEDVDELE-KRTLERLSTK------YAKDD 155

Query: 134 YE--NKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSA 193
           YE  + N   +  L PS RD   LW VKC +GRER+   CLMQK VD    G++ +I+SA
Sbjct: 156 YELDDVNDVDQQALLPSVRDP-KLWLVKCAIGREREVAVCLMQKIVDR---GSEFKIRSA 215

Query: 194 FYVDHVKGFIYIEAPRQYDLIEACKGISGIYST-RIASVPENDISQLLTVRSRVSEVSVG 253
             +DH++ ++YIEA  +  + EA KG+  IY+  +I  VP  +++ +L+V S+  ++S  
Sbjct: 216 IALDHLQNYVYIEADMEAHVKEAIKGMRNIYANQKILLVPIKEMTAVLSVESKAIDLSRD 275

Query: 254 TMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGV-AAKKTTNPAPR 313
           +  R+K G YKGDLAQ+V V+N RKR TVKL+PRIDLQA+A K  G     KK   P PR
Sbjct: 276 SWVRMKLGIYKGDLAQVVDVDNVRKRVTVKLIPRIDLQALANKLEGTENVKKKAFAPPPR 335

Query: 314 LINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDEL 373
            +N  E  E    ++ RRD  TG  FE + GM+ KDG+L+KK+S  S+    V P+ DEL
Sbjct: 336 FMNIDEARELHIRVEHRRDPMTGDYFENIGGMLFKDGFLYKKVSTKSIAAQNVTPTFDEL 395

Query: 374 LKFKPPESNESNDLEWLSQLYGEKRK-----------------------------KKIIR 433
            +FK P  N   D    S L+  ++K                               +IR
Sbjct: 396 ERFKRPNENGEIDFVDESTLFANRKKGHFMKGDAVIVIKGDLKNLKGWIEKVDEENVLIR 455

Query: 434 TE-------------------------------KGGGKG--------------------- 493
           +E                                 GG G                     
Sbjct: 456 SEMKDLPNPIAVNGRELCKYFEPGNFVKVVSGIHEGGTGMIVKVDQHMLIILSDTTKEHI 515

Query: 494 -----------EGTSGSSSMNSFADHDLVCFGRKDFGMILGTEKDDSYKILKEGPDGSIV 553
                      E T G + +  +  HDLV      FG+IL  +  ++ +ILK  PD S V
Sbjct: 516 CVFADHVAKSAEVTKGVTKIGDYELHDLVILSDFSFGVILKLD-SEAIQILKGVPDSSEV 575

Query: 554 VNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVFVYDE 613
             V+  E+K   +  K    D    +++V D V+V+EG  K KQG V  +Y+  +F++D 
Sbjct: 576 SIVKASEIKY-KIWKKINVQDRYKNVVAVKDVVRVIEGPSKGKQGPVVQIYKGVLFIHDR 635

Query: 614 NEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWAEKDS 641
           + +++ G+ C + + C     ++  P+            D   +P +             
Sbjct: 636 HNLEHTGFICTRCSSCVLAGGNFKTPALVPPSPRRFQRADMGYNPGA-------GGRHQG 695

BLAST of MS010097 vs. ExPASy TrEMBL
Match: A0A6J1BZ56 (protein RNA-directed DNA methylation 3 OS=Momordica charantia OX=3673 GN=LOC111006022 PE=4 SV=1)

HSP 1 Score: 2241.8 bits (5808), Expect = 0.0e+00
Identity = 1206/1317 (91.57%), Postives = 1215/1317 (92.26%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD
Sbjct: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN
Sbjct: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH
Sbjct: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI
Sbjct: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS
Sbjct: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK 600
            DFS SPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK
Sbjct: 541  DFSFSPKSPLSPKKPWAEKDSGREYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKK 600

Query: 601  DVTVKLDSQQKVLTVRSDFLSEVYRKTSTV---EDTEFGSLKPFDILGNEGSSQDWMGGT 660
            DVTVKLDSQQKVLTVRSDFLSEVYRKTSTV   EDTEFGSLKPFDILGNEGSSQDWMGGT
Sbjct: 601  DVTVKLDSQQKVLTVRSDFLSEVYRKTSTVSLSEDTEFGSLKPFDILGNEGSSQDWMGGT 660

Query: 661  GSSTGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASM-IITDVEDSPWV 720
            GSSTGGDGWNSAGPSSERSPWPSFPESGTS     P SS + F   S+    DVEDSPWV
Sbjct: 661  GSSTGGDGWNSAGPSSERSPWPSFPESGTS---NCPGSSSNPFGSESLDAQKDVEDSPWV 720

Query: 721  SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG 780
            SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG
Sbjct: 721  SKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPG 780

Query: 781  GDY------------------------------------------KDSESGGWKKSQNAS 840
            GD                                           KDSESGGWKKSQNAS
Sbjct: 781  GDCAGPTDQAEDKWDKGKRVSSDNQTGNWGDGTSGKNEPSAWSRDKDSESGGWKKSQNAS 840

Query: 841  FGDENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN 900
            FGDENNSAEASA+KWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN
Sbjct: 841  FGDENNSAEASADKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWN 900

Query: 901  TQKQESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGW 960
            TQKQESNR SWGKPSDRGGTRSSESGRGGNQVRGGS GQDSSSDWNKSTTTDGAGKKDGW
Sbjct: 901  TQKQESNRGSWGKPSDRGGTRSSESGRGGNQVRGGSSGQDSSSDWNKSTTTDGAGKKDGW 960

Query: 961  NKPNLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS 1020
            NKPNLASEDESIGKKGWGQGNEA+DSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS
Sbjct: 961  NKPNLASEDESIGKKGWGQGNEANDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKS 1020

Query: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080
            SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG
Sbjct: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080

Query: 1081 KDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA 1140
            KDGERKD+GSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA
Sbjct: 1081 KDGERKDQGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSSWA 1140

Query: 1141 EKSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG 1200
            +KSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG
Sbjct: 1141 KKSNWNSGNESNDNQFTGDTSGHGSWGGDGSDRGGFRGRGGFRGRGERGRFGGRGRSDRG 1200

Query: 1201 GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWEKSGSDRGGFGGGRGRWSSGGG 1260
            GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWE                     
Sbjct: 1201 GSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGGDWE--------------------- 1260

Query: 1261 GSDSGWGKSGSDRGGFGGGRGRGRWNQGDSNSNDGGNGGWSSSGGRGGFGGGRGRGR 1272
                   KSGSDRGGFGGGRGRGRWNQ ++ SNDG +GGWS     GG GG RGRGR
Sbjct: 1261 -------KSGSDRGGFGGGRGRGRWNQ-EAGSNDGDSGGWS-----GGGGGHRGRGR 1280

BLAST of MS010097 vs. ExPASy TrEMBL
Match: A0A6J1H340 (protein RNA-directed DNA methylation 3-like OS=Cucurbita moschata OX=3662 GN=LOC111459247 PE=4 SV=1)

HSP 1 Score: 1900.6 bits (4922), Expect = 0.0e+00
Identity = 1147/1652 (69.43%), Postives = 1250/1652 (75.67%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKDTS+GKRK R+D + +  ARKRRDRSVLQFFEDV+PE+G  SDDSDFLDD
Sbjct: 1    MASKGKGIAKDTSAGKRKLRDDTD-SSAARKRRDRSVLQFFEDVSPELGAYSDDSDFLDD 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEEEF+  PAFKND +K QNIPFFPKEEEMNEEEFDR+MEEHY +GPGLGAFAEENYEN
Sbjct: 61   FMEEEFEPVPAFKNDDSKAQNIPFFPKEEEMNEEEFDRMMEEHYTRGPGLGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            K STGRNP Q S+RD ISLWKVKCMVG ERQSVFCLMQKFVDLHSFGTKLQIKSAF V+H
Sbjct: 121  KISTGRNPPQQSARDNISLWKVKCMVGHERQSVFCLMQKFVDLHSFGTKLQIKSAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            +KGFIYIEAPRQYDLIEACKGI+GIYSTRIASVPENDISQLLTV+SRVSEV+VGTMARVK
Sbjct: 181  IKGFIYIEAPRQYDLIEACKGINGIYSTRIASVPENDISQLLTVQSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKL+PRIDLQ+MA KFGGGV AKK TNPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLIPRIDLQSMAEKFGGGVVAKKATNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            +EFRPLMQFRRDRETGKLFEF DGMMLKDGYL+KK+SLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  EEFRPLMQFRRDRETGKLFEFFDGMMLKDGYLYKKVSLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNE+NDLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSSM SF DHDLVCFGRKDFGMI
Sbjct: 361  SNEANDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSMISFGDHDLVCFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LGTEKDDSYKILK+GPDGS+VVNVQRKELKSGPL+ KFT+ D NGKIISVSDNVKVLEGS
Sbjct: 421  LGTEKDDSYKILKDGPDGSVVVNVQRKELKSGPLDAKFTSVDLNGKIISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSN+C KIKISYDAPSGK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNLCAKIKISYDAPSGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSP+KPWAEK+ GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAV K
Sbjct: 541  DFSSSPKSPLSPQKPWAEKE-GREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVHK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTV---EDTEFGSLKPFDILGNEGSSQDWMGG 660
            +DVTVKLDSQQKVLTVRSD L+EV+RK+S V   ED EF SLKPFDILGNEGSSQDWMGG
Sbjct: 601  RDVTVKLDSQQKVLTVRSDLLAEVHRKSSAVSVSEDPEFSSLKPFDILGNEGSSQDWMGG 660

Query: 661  TGSSTGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIIT--DVEDSP 720
            TGSS   DGWNSAGPSSERSPWPSFPESGT      P SS ++ + +  +    DVEDSP
Sbjct: 661  TGSSAAADGWNSAGPSSERSPWPSFPESGT---LNCPGSSSTNPFGSENLDAKKDVEDSP 720

Query: 721  WVSKTVPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVV 780
            WVSK+   A+TSWGAA S+V DT NDDQA GWGKSESWDKATAKT SDGN SGAW K VV
Sbjct: 721  WVSKSTAEANTSWGAANSSV-DTANDDQACGWGKSESWDKATAKTISDGNVSGAWGKSVV 780

Query: 781  PGGDY------------------------------------KDSESGGWKKSQNASFGDE 840
            P GD                                     KDSESGGWKK+ + S GD 
Sbjct: 781  PSGDSAGDKWDKGKHVTSDNQTGKWGGGTSGKNELSAWSRDKDSESGGWKKNPSVSVGDN 840

Query: 841  NNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQKQ 900
            N  AE SA+KW SKNRS+G WGD N STTVSEI+ AGK N G                  
Sbjct: 841  NTPAETSADKWGSKNRSNGSWGDQNVSTTVSEIQTAGKDNVG------------------ 900

Query: 901  ESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKPN 960
                  W KP                    G+  + + S WN+ T+  G G+   W    
Sbjct: 901  -----GWTKP--------------------GAESKINPSGWNEDTSMKG-GQTSNW---- 960

Query: 961  LASEDESIGKKGWGQGNEASDSGNK-WQSSGS--DGGKKW-GTNESERDGGNKSWNTSKS 1020
              + DE+    GWG+     D GN  W  S +  DG   W  +NESER+GGN+SWN SKS
Sbjct: 961  -GNRDEA---GGWGKPMNVGDGGNSAWNKSTACGDGNDSWKKSNESEREGGNRSWNASKS 1020

Query: 1021 SDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESG 1080
            SDGD     SAIWK+KSDSSSLTA KGDQWGG W+KQHSSN+TKASEDNSPWNKKSVESG
Sbjct: 1021 SDGD-----SAIWKEKSDSSSLTASKGDQWGGGWNKQHSSNETKASEDNSPWNKKSVESG 1080

Query: 1081 KDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS-- 1140
            KD E +++GSGWNVGK+S GDSASGWGQTS+EAGSSDQ  SWGSNWKK S+ GNEDSS  
Sbjct: 1081 KDNELENQGSGWNVGKTS-GDSASGWGQTSREAGSSDQGSSWGSNWKKKSDTGNEDSSSA 1140

Query: 1141 -------------WAEKSNWNSGNESNDNQFT----GDTSGHGSWGGDGSDRGGFRGRGG 1200
                         W EKSNWNSGNE N N         T     W G+ SDRGGFRGRG 
Sbjct: 1141 KKSNWSSGSGNSNWGEKSNWNSGNEFNANHSNDGAEAQTEVSNDWRGESSDRGGFRGRGS 1200

Query: 1201 FRGRGERGRFGGRGRSD-----RGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG 1260
            FRGRGERGRFGGRGRSD     RGGSDRGGFGGRGRGRWNSEGGSNDG+N+GWSS  GGG
Sbjct: 1201 FRGRGERGRFGGRGRSDRGGFGRGGSDRGGFGGRGRGRWNSEGGSNDGDNKGWSS--GGG 1260

Query: 1261 DWEKSGSDRGGFGG-GRGRWSSGGGGSDS---GWG-------KSGSDRGGFGGGRGRGRW 1320
            DWEKS SDRGGFGG GRGRW+   G +D    GW        +SGSDRGGF  GRGRGRW
Sbjct: 1261 DWEKSSSDRGGFGGRGRGRWNRESGSNDGENRGWSSGGGDRERSGSDRGGF-RGRGRGRW 1320

Query: 1321 NQGDSNSNDGGN-GGWSSSGGRGGFGGGRGRGRWNQEGDSS------------------- 1380
            NQ +  S DG N GGW  SGGRGG+ GGRGRGRWNQEG S+                   
Sbjct: 1321 NQ-EGGSRDGDNGGGW--SGGRGGY-GGRGRGRWNQEGGSNDGGDSVGWGGGGNWGKSGS 1380

Query: 1381 ------------------SKDSDNGGWSGGGGG-WGSSGSGRGGIGGRGRGRRNQDGG-- 1440
                              S +SD GGWSGGGGG WG SGS RGG GGRGRGRRNQDGG  
Sbjct: 1381 DRGGFGGGGSGKWSQEGGSNNSDYGGWSGGGGGSWGKSGSDRGGFGGRGRGRRNQDGGLK 1440

Query: 1441 SNDNEDKPASWSTGSGNTGGWSSSGG-AGSWSRGGDEKNQQQSWKTNNEGGDQSGGQGSG 1500
             +DN DKPASWSTGSGN GGW S GG AGSW+ GGD+KNQQQSWK++N+      G+GSG
Sbjct: 1441 DDDNVDKPASWSTGSGNNGGWGSGGGTAGSWNGGGDDKNQQQSWKSSND-----AGEGSG 1500

Query: 1501 WKEPSGNEQNTWKSSGA------SGWNKSSAGKDIEESCSQKSSWGQAAAKTDAQAGGGW 1521
            WK PSGNE   WK+SG+      SGWNKS+A K+IEES  QK+S G+AA       GGGW
Sbjct: 1501 WKAPSGNENINWKASGSSGVDDNSGWNKSTACKEIEESGDQKNSRGKAAKADIHIQGGGW 1560

BLAST of MS010097 vs. ExPASy TrEMBL
Match: A0A6J1JRE1 (protein RNA-directed DNA methylation 3-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111489134 PE=4 SV=1)

HSP 1 Score: 1899.0 bits (4918), Expect = 0.0e+00
Identity = 1144/1681 (68.05%), Postives = 1234/1681 (73.41%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKD S GKRK R+DNN +  ARKRRDRSVLQFFEDVAPE GGESDDSDF D 
Sbjct: 1    MASKGKGIAKDASPGKRKLRDDNN-SSAARKRRDRSVLQFFEDVAPEFGGESDDSDFFDG 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEE+F+T PAFKND AK QNIPFFPKEEEMNEEEFDR+MEEHYNQGPG GAFAEENYEN
Sbjct: 61   FMEEDFETIPAFKNDDAKSQNIPFFPKEEEMNEEEFDRMMEEHYNQGPGFGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNP   S+RDTISLWKVKCMVGRERQSVFCLMQKFVDL+SFGTKLQIK+AF V+H
Sbjct: 121  KNSTGRNPPLQSARDTISLWKVKCMVGRERQSVFCLMQKFVDLNSFGTKLQIKAAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTR+ASVPENDISQLLTVRSRVSEV+VGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRVASVPENDISQLLTVRSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMA KFGGG AAKKT+NPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAEKFGGGSAAKKTSNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNES+DLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSS +SF DHDL+CFGRKDFGMI
Sbjct: 361  SNESHDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSTSSFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LG EKDDSYKILK+ P+GS+VVNVQRKELK G L+ KFTAADHNGK+ISVSDNVKVLEGS
Sbjct: 421  LGMEKDDSYKILKDSPNGSVVVNVQRKELKGGSLDAKFTAADHNGKMISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEV+NDGYFCCKSN CEKIKISYDAP GK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVENDGYFCCKSNKCEKIKISYDAPCGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK++GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKETGREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTVEDTEFGSLKPFDILGNEGSSQDWMGGTGS 660
            +DVTVKLDSQQKVLTVRSDFLSEV RK+S V  +E   LKPFDILGNEGSSQDWMGGTGS
Sbjct: 601  RDVTVKLDSQQKVLTVRSDFLSEVQRKSSAVSLSE-DPLKPFDILGNEGSSQDWMGGTGS 660

Query: 661  STGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKT 720
            S GGDGWNS GPSSE +PWPSFPES T       SSS +     S      EDSPWVSK+
Sbjct: 661  SAGGDGWNSTGPSSEGNPWPSFPESSTLNG--PGSSSTNPIGSESFDANKDEDSPWVSKS 720

Query: 721  VPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDY 780
             P+ASTSWGAAKS+V DT N+ QA GWGKS+SW K  AKT SDGNASGAW K  VP GD 
Sbjct: 721  TPDASTSWGAAKSSV-DTANNGQASGWGKSDSWGKTIAKTCSDGNASGAWGKTAVPSGDS 780

Query: 781  ------------------------------------------KDSESGGWKKSQNASFGD 840
                                                      KD+ESGGWKK+Q+A+F D
Sbjct: 781  AGLTENTWDKWDKGKQVSSDNQTGNWDNGTSGKNEHSAWSRDKDAESGGWKKTQSANFDD 840

Query: 841  ENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQK 900
            +   AE           S+G W             P  ++    S WN         T  
Sbjct: 841  DKTPAE-----------SAGDW-----------TNPEAENKVNPSGWN-------EGTSM 900

Query: 901  QESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKP 960
            + S  S+WG   + GG      G+  N   GG      SS WNKST+ DGA + D WNKP
Sbjct: 901  KGSQTSNWGNQDETGGW-----GKPKNVGNGG------SSAWNKSTSGDGAVENDSWNKP 960

Query: 961  NLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKSSDG 1020
             L S DESIGKKGWGQ NEASD+GNKWQSS SDGG KWGTNESE +GG            
Sbjct: 961  KLFSHDESIGKKGWGQSNEASDNGNKWQSSRSDGGTKWGTNESEHEGG------------ 1020

Query: 1021 DTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESGKDG 1080
                      KDKSDSSSLT P+GDQ  G WDKQ SSNDTKASE+NSPWNKKSVESGKDG
Sbjct: 1021 ----------KDKSDSSSLTTPRGDQSVGGWDKQRSSNDTKASEENSPWNKKSVESGKDG 1080

Query: 1081 ERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS----- 1140
            E K++GSGWNVGK+SGGDSASGWGQ SKEAGSSD  G+WGSNWKKNS+ GNEDSS     
Sbjct: 1081 ELKNQGSGWNVGKTSGGDSASGWGQASKEAGSSDLVGNWGSNWKKNSDVGNEDSSLAKKS 1140

Query: 1141 ----------WAEKSNWNSGNESNDNQFTG----------DTSGHGSWGGDGSDRGGFRG 1200
                      W EKSNWNSGNE N N  TG          DTSG+GSW G+ SDRGG+RG
Sbjct: 1141 NWSSGSGNSNWGEKSNWNSGNEYNANHSTGGAEAQTDVSNDTSGYGSWRGENSDRGGYRG 1200

Query: 1201 RGGFRGRGERGRFGGRGRSDRGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG-- 1260
            RGGFRGRGERGRFGGRGRSDRGG  RGGFGGRGRGRWNSEGGSN G+N+GWSSGGGGG  
Sbjct: 1201 RGGFRGRGERGRFGGRGRSDRGGFGRGGFGGRGRGRWNSEGGSNGGDNKGWSSGGGGGDN 1260

Query: 1261 ------------------------------------------------------------ 1320
                                                                        
Sbjct: 1261 KGWSSGGGGGDNRGWSSGGGGDDNKGWSSGGAGDNKGWGGGGGGDNKGWSSGGGDNKGWS 1320

Query: 1321 --------DWEKSGSDRGGFGG-GRGRWSSGGGGSDSGWGKSGSDRGGFGGGRGRGRWNQ 1380
                    DWEKSGSDRGGFGG GRGRWSSG G  D    K GSDRGGF  GRGRGRWNQ
Sbjct: 1321 GGGGGGSSDWEKSGSDRGGFGGRGRGRWSSGSGSHDGDREKFGSDRGGF-RGRGRGRWNQ 1380

Query: 1381 GDSNSNDGGNGGWSSSGGRGGFGGGRGRGRWNQEGDSSSKDSDNGGW-SGGGGGWGSSGS 1440
             D  S+DG NGGW   GGRGG+ GGRGRGRWNQEGDS+  D+  GGW SG GG WG SGS
Sbjct: 1381 -DGGSHDGDNGGW--RGGRGGY-GGRGRGRWNQEGDSNDGDNGGGGWSSGNGGSWGKSGS 1440

Query: 1441 G-RGGIGGRGRGRRNQDGGSNDNEDKPASWSTGSGNTGGWSSSGGAGSWSRGGDEKNQQQ 1500
               GG GGRG G+ NQ+GGSN+N              GGWSS GGAGSW++GGDEKNQ Q
Sbjct: 1441 DIGGGFGGRGSGKWNQEGGSNNN--------------GGWSSGGGAGSWNQGGDEKNQAQ 1500

Query: 1501 SWKTNNEGGDQSGG-QGSGWKEP-SGNEQNTWKSSGA------SGWNKSSAGKDIEESCS 1521
             WK +N+GGD+ GG QGSGWKEP SGNE N WKSSG+      SGWNKS+ GK++EES S
Sbjct: 1501 GWKPSNDGGDKFGGQQGSGWKEPTSGNEPNNWKSSGSSGADHNSGWNKSTTGKEMEESDS 1560

BLAST of MS010097 vs. ExPASy TrEMBL
Match: A0A6J1JYW2 (protein RNA-directed DNA methylation 3-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111489134 PE=4 SV=1)

HSP 1 Score: 1899.0 bits (4918), Expect = 0.0e+00
Identity = 1144/1681 (68.05%), Postives = 1234/1681 (73.41%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKD S GKRK R+DNN +  ARKRRDRSVLQFFEDVAPE GGESDDSDF D 
Sbjct: 1    MASKGKGIAKDASPGKRKLRDDNN-SSAARKRRDRSVLQFFEDVAPEFGGESDDSDFFDG 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEE+F+T PAFKND AK QNIPFFPKEEEMNEEEFDR+MEEHYNQGPG GAFAEENYEN
Sbjct: 61   FMEEDFETIPAFKNDDAKSQNIPFFPKEEEMNEEEFDRMMEEHYNQGPGFGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNP   S+RDTISLWKVKCMVGRERQSVFCLMQKFVDL+SFGTKLQIK+AF V+H
Sbjct: 121  KNSTGRNPPLQSARDTISLWKVKCMVGRERQSVFCLMQKFVDLNSFGTKLQIKAAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTR+ASVPENDISQLLTVRSRVSEV+VGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRVASVPENDISQLLTVRSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMA KFGGG AAKKT+NPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAEKFGGGSAAKKTSNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNES+DLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSS +SF DHDL+CFGRKDFGMI
Sbjct: 361  SNESHDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSTSSFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LG EKDDSYKILK+ P+GS+VVNVQRKELK G L+ KFTAADHNGK+ISVSDNVKVLEGS
Sbjct: 421  LGMEKDDSYKILKDSPNGSVVVNVQRKELKGGSLDAKFTAADHNGKMISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEV+NDGYFCCKSN CEKIKISYDAP GK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVENDGYFCCKSNKCEKIKISYDAPCGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK++GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKETGREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTVEDTEFGSLKPFDILGNEGSSQDWMGGTGS 660
            +DVTVKLDSQQKVLTVRSDFLSEV RK+S V  +E   LKPFDILGNEGSSQDWMGGTGS
Sbjct: 601  RDVTVKLDSQQKVLTVRSDFLSEVQRKSSAVSLSE-DPLKPFDILGNEGSSQDWMGGTGS 660

Query: 661  STGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKT 720
            S GGDGWNS GPSSE +PWPSFPES T       SSS +     S      EDSPWVSK+
Sbjct: 661  SAGGDGWNSTGPSSEGNPWPSFPESSTLNG--PGSSSTNPIGSESFDANKDEDSPWVSKS 720

Query: 721  VPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDY 780
             P+ASTSWGAAKS+V DT N+ QA GWGKS+SW K  AKT SDGNASGAW K  VP GD 
Sbjct: 721  TPDASTSWGAAKSSV-DTANNGQASGWGKSDSWGKTIAKTCSDGNASGAWGKTAVPSGDS 780

Query: 781  ------------------------------------------KDSESGGWKKSQNASFGD 840
                                                      KD+ESGGWKK+Q+A+F D
Sbjct: 781  AGLTENTWDKWDKGKQVSSDNQTGNWDNGTSGKNEHSAWSRDKDAESGGWKKTQSANFDD 840

Query: 841  ENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQK 900
            +   AE           S+G W             P  ++    S WN         T  
Sbjct: 841  DKTPAE-----------SAGDW-----------TNPEAENKVNPSGWN-------EGTSM 900

Query: 901  QESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKP 960
            + S  S+WG   + GG      G+  N   GG      SS WNKST+ DGA + D WNKP
Sbjct: 901  KGSQTSNWGNQDETGGW-----GKPKNVGNGG------SSAWNKSTSGDGAVENDSWNKP 960

Query: 961  NLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKSSDG 1020
             L S DESIGKKGWGQ NEASD+GNKWQSS SDGG KWGTNESE +GG            
Sbjct: 961  KLFSHDESIGKKGWGQSNEASDNGNKWQSSRSDGGTKWGTNESEHEGG------------ 1020

Query: 1021 DTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESGKDG 1080
                      KDKSDSSSLT P+GDQ  G WDKQ SSNDTKASE+NSPWNKKSVESGKDG
Sbjct: 1021 ----------KDKSDSSSLTTPRGDQSVGGWDKQRSSNDTKASEENSPWNKKSVESGKDG 1080

Query: 1081 ERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS----- 1140
            E K++GSGWNVGK+SGGDSASGWGQ SKEAGSSD  G+WGSNWKKNS+ GNEDSS     
Sbjct: 1081 ELKNQGSGWNVGKTSGGDSASGWGQASKEAGSSDLVGNWGSNWKKNSDVGNEDSSLAKKS 1140

Query: 1141 ----------WAEKSNWNSGNESNDNQFTG----------DTSGHGSWGGDGSDRGGFRG 1200
                      W EKSNWNSGNE N N  TG          DTSG+GSW G+ SDRGG+RG
Sbjct: 1141 NWSSGSGNSNWGEKSNWNSGNEYNANHSTGGAEAQTDVSNDTSGYGSWRGENSDRGGYRG 1200

Query: 1201 RGGFRGRGERGRFGGRGRSDRGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG-- 1260
            RGGFRGRGERGRFGGRGRSDRGG  RGGFGGRGRGRWNSEGGSN G+N+GWSSGGGGG  
Sbjct: 1201 RGGFRGRGERGRFGGRGRSDRGGFGRGGFGGRGRGRWNSEGGSNGGDNKGWSSGGGGGDN 1260

Query: 1261 ------------------------------------------------------------ 1320
                                                                        
Sbjct: 1261 KGWSSGGGGGDNRGWSSGGGGDDNKGWSSGGAGDNKGWGGGGGGDNKGWSSGGGDNKGWS 1320

Query: 1321 --------DWEKSGSDRGGFGG-GRGRWSSGGGGSDSGWGKSGSDRGGFGGGRGRGRWNQ 1380
                    DWEKSGSDRGGFGG GRGRWSSG G  D    K GSDRGGF  GRGRGRWNQ
Sbjct: 1321 GGGGGGSSDWEKSGSDRGGFGGRGRGRWSSGSGSHDGDREKFGSDRGGF-RGRGRGRWNQ 1380

Query: 1381 GDSNSNDGGNGGWSSSGGRGGFGGGRGRGRWNQEGDSSSKDSDNGGW-SGGGGGWGSSGS 1440
             D  S+DG NGGW   GGRGG+ GGRGRGRWNQEGDS+  D+  GGW SG GG WG SGS
Sbjct: 1381 -DGGSHDGDNGGW--RGGRGGY-GGRGRGRWNQEGDSNDGDNGGGGWSSGNGGSWGKSGS 1440

Query: 1441 G-RGGIGGRGRGRRNQDGGSNDNEDKPASWSTGSGNTGGWSSSGGAGSWSRGGDEKNQQQ 1500
               GG GGRG G+ NQ+GGSN+N              GGWSS GGAGSW++GGDEKNQ Q
Sbjct: 1441 DIGGGFGGRGSGKWNQEGGSNNN--------------GGWSSGGGAGSWNQGGDEKNQAQ 1500

Query: 1501 SWKTNNEGGDQSGG-QGSGWKEP-SGNEQNTWKSSGA------SGWNKSSAGKDIEESCS 1521
             WK +N+GGD+ GG QGSGWKEP SGNE N WKSSG+      SGWNKS+ GK++EES S
Sbjct: 1501 GWKPSNDGGDKFGGQQGSGWKEPTSGNEPNNWKSSGSSGADHNSGWNKSTTGKEMEESDS 1560

BLAST of MS010097 vs. ExPASy TrEMBL
Match: A0A6J1JV02 (protein RNA-directed DNA methylation 3-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111489134 PE=4 SV=1)

HSP 1 Score: 1897.1 bits (4913), Expect = 0.0e+00
Identity = 1143/1678 (68.12%), Postives = 1233/1678 (73.48%), Query Frame = 0

Query: 1    MASKGKGIAKDTSSGKRKQREDNNGADLARKRRDRSVLQFFEDVAPEVGGESDDSDFLDD 60
            MASKGKGIAKD S GKRK R+DNN +  ARKRRDRSVLQFFEDVAPE GGESDDSDF D 
Sbjct: 1    MASKGKGIAKDASPGKRKLRDDNN-SSAARKRRDRSVLQFFEDVAPEFGGESDDSDFFDG 60

Query: 61   FMEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEENYEN 120
            FMEE+F+T PAFKND AK QNIPFFPKEEEMNEEEFDR+MEEHYNQGPG GAFAEENYEN
Sbjct: 61   FMEEDFETIPAFKNDDAKSQNIPFFPKEEEMNEEEFDRMMEEHYNQGPGFGAFAEENYEN 120

Query: 121  KNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSAFYVDH 180
            KNSTGRNP   S+RDTISLWKVKCMVGRERQSVFCLMQKFVDL+SFGTKLQIK+AF V+H
Sbjct: 121  KNSTGRNPPLQSARDTISLWKVKCMVGRERQSVFCLMQKFVDLNSFGTKLQIKAAFCVEH 180

Query: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSRVSEVSVGTMARVK 240
            VKGFIYIEAPRQYDLIEACKGISGIYSTR+ASVPENDISQLLTVRSRVSEV+VGTMARVK
Sbjct: 181  VKGFIYIEAPRQYDLIEACKGISGIYSTRVASVPENDISQLLTVRSRVSEVAVGTMARVK 240

Query: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKTTNPAPRLINSSEL 300
            NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMA KFGGG AAKKT+NPAPRLINSSEL
Sbjct: 241  NGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAEKFGGGSAAKKTSNPAPRLINSSEL 300

Query: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDELLKFKPPE 360
            DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYL+KKISLDSLNCWGVMPSEDELLKFKPPE
Sbjct: 301  DEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLYKKISLDSLNCWGVMPSEDELLKFKPPE 360

Query: 361  SNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGSSSMNSFADHDLVCFGRKDFGMI 420
            SNES+DLEWLSQLYGEK+KKKIIRTEKGGGKGEG+SGSSS +SF DHDL+CFGRKDFGMI
Sbjct: 361  SNESHDLEWLSQLYGEKKKKKIIRTEKGGGKGEGSSGSSSTSSFGDHDLICFGRKDFGMI 420

Query: 421  LGTEKDDSYKILKEGPDGSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGS 480
            LG EKDDSYKILK+ P+GS+VVNVQRKELK G L+ KFTAADHNGK+ISVSDNVKVLEGS
Sbjct: 421  LGMEKDDSYKILKDSPNGSVVVNVQRKELKGGSLDAKFTAADHNGKMISVSDNVKVLEGS 480

Query: 481  LKDKQGIVKHVYRHTVFVYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFE 540
            LKDKQGIVKHVYRHTVFVYDENEV+NDGYFCCKSN CEKIKISYDAP GK+DDKGFSGFE
Sbjct: 481  LKDKQGIVKHVYRHTVFVYDENEVENDGYFCCKSNKCEKIKISYDAPCGKEDDKGFSGFE 540

Query: 541  DFSSSPKSPLSPKKPWAEKDSGREYNRDDK-DGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600
            DFSSSPKSPLSPKKPWAEK++GREYNRDD+ DGMFSIGQTLRIRVGPLKGYLCRVIAVRK
Sbjct: 541  DFSSSPKSPLSPKKPWAEKETGREYNRDDRGDGMFSIGQTLRIRVGPLKGYLCRVIAVRK 600

Query: 601  KDVTVKLDSQQKVLTVRSDFLSEVYRKTSTVEDTEFGSLKPFDILGNEGSSQDWMGGTGS 660
            +DVTVKLDSQQKVLTVRSDFLSEV RK+S V  +E   LKPFDILGNEGSSQDWMGGTGS
Sbjct: 601  RDVTVKLDSQQKVLTVRSDFLSEVQRKSSAVSLSE-DPLKPFDILGNEGSSQDWMGGTGS 660

Query: 661  STGGDGWNSAGPSSERSPWPSFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKT 720
            S GGDGWNS GPSSE +PWPSFPES T       SSS +     S      EDSPWVSK+
Sbjct: 661  SAGGDGWNSTGPSSEGNPWPSFPESSTLNG--PGSSSTNPIGSESFDANKDEDSPWVSKS 720

Query: 721  VPNASTSWGAAKSNVDDTVNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDY 780
             P+ASTSWGAAKS+V DT N+ QA GWGKS+SW K  AKT SDGNASGAW K  VP GD 
Sbjct: 721  TPDASTSWGAAKSSV-DTANNGQASGWGKSDSWGKTIAKTCSDGNASGAWGKTAVPSGDS 780

Query: 781  ------------------------------------------KDSESGGWKKSQNASFGD 840
                                                      KD+ESGGWKK+Q+A+F D
Sbjct: 781  AGLTENTWDKWDKGKQVSSDNQTGNWDNGTSGKNEHSAWSRDKDAESGGWKKTQSANFDD 840

Query: 841  ENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSWNTQK 900
            +   AE           S+G W             P  ++    S WN         T  
Sbjct: 841  DKTPAE-----------SAGDW-----------TNPEAENKVNPSGWN-------EGTSM 900

Query: 901  QESNRSSWGKPSDRGGTRSSESGRGGNQVRGGSFGQDSSSDWNKSTTTDGAGKKDGWNKP 960
            + S  S+WG   + GG      G+  N   GG      SS WNKST+ DGA + D WNKP
Sbjct: 901  KGSQTSNWGNQDETGGW-----GKPKNVGNGG------SSAWNKSTSGDGAVENDSWNKP 960

Query: 961  NLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGGKKWGTNESERDGGNKSWNTSKSSDG 1020
             L S DESIGKKGWGQ NEASD+GNKWQSS SDGG KWGTNESE +GG            
Sbjct: 961  KLFSHDESIGKKGWGQSNEASDNGNKWQSSRSDGGTKWGTNESEHEGG------------ 1020

Query: 1021 DTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQHSSNDTKASEDNSPWNKKSVESGKDG 1080
                      KDKSDSSSLT P+GDQ  G WDKQ SSNDTKASE+NSPWNKKSVESGKDG
Sbjct: 1021 ----------KDKSDSSSLTTPRGDQSVGGWDKQRSSNDTKASEENSPWNKKSVESGKDG 1080

Query: 1081 ERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSSDQAGSWGSNWKKNSNAGNEDSS----- 1140
            E K++GSGWNVGK+SGGDSASGWGQ SKEAGSSD  G+WGSNWKKNS+ GNEDSS     
Sbjct: 1081 ELKNQGSGWNVGKTSGGDSASGWGQASKEAGSSDLVGNWGSNWKKNSDVGNEDSSLAKKS 1140

Query: 1141 ----------WAEKSNWNSGNESNDNQFTG----------DTSGHGSWGGDGSDRGGFRG 1200
                      W EKSNWNSGNE N N  TG          DTSG+GSW G+ SDRGG+RG
Sbjct: 1141 NWSSGSGNSNWGEKSNWNSGNEYNANHSTGGAEAQTDVSNDTSGYGSWRGENSDRGGYRG 1200

Query: 1201 RGGFRGRGERGRFGGRGRSDRGGSDRGGFGGRGRGRWNSEGGSNDGENRGWSSGGGGG-- 1260
            RGGFRGRGERGRFGGRGRSDRGG  RGGFGGRGRGRWNSEGGSN G+N+GWSSGGGGG  
Sbjct: 1201 RGGFRGRGERGRFGGRGRSDRGGFGRGGFGGRGRGRWNSEGGSNGGDNKGWSSGGGGGDN 1260

Query: 1261 ------------------------------------------------------------ 1320
                                                                        
Sbjct: 1261 KGWSSGGGGGDNRGWSSGGGGDDNKGWSSGGAGDNKGWGGGGGGDNKGWSSGGGDNKGWS 1320

Query: 1321 --------DWEKSGSDRGGFGG-GRGRWSSGGGGSDSGWGKSGSDRGGFGGGRGRGRWNQ 1380
                    DWEKSGSDRGGFGG GRGRWSSG G  D    K GSDRGGF  GRGRGRWNQ
Sbjct: 1321 GGGGGGSSDWEKSGSDRGGFGGRGRGRWSSGSGSHDGDREKFGSDRGGF-RGRGRGRWNQ 1380

Query: 1381 GDSNSNDGGNGGWSSSGGRGGFGGGRGRGRWNQEGDSSSKDSDNGGW-SGGGGGWGSSGS 1440
             D  S+DG NGGW   GGRGG+ GGRGRGRWNQEGDS+  D+  GGW SG GG WG SGS
Sbjct: 1381 -DGGSHDGDNGGW--RGGRGGY-GGRGRGRWNQEGDSNDGDNGGGGWSSGNGGSWGKSGS 1440

Query: 1441 G-RGGIGGRGRGRRNQDGGSNDNEDKPASWSTGSGNTGGWSSSGGAGSWSRGGDEKNQQQ 1500
               GG GGRG G+ NQ+GGSN+N              GGWSS GGAGSW++GGDEKNQ Q
Sbjct: 1441 DIGGGFGGRGSGKWNQEGGSNNN--------------GGWSSGGGAGSWNQGGDEKNQAQ 1500

Query: 1501 SWKTNNEGGDQSGG-QGSGWKEP-SGNEQNTWKSSGA------SGWNKSSAGKDIEESCS 1518
             WK +N+GGD+ GG QGSGWKEP SGNE N WKSSG+      SGWNKS+ GK++EES S
Sbjct: 1501 GWKPSNDGGDKFGGQQGSGWKEPTSGNEPNNWKSSGSSGADHNSGWNKSTTGKEMEESDS 1560

BLAST of MS010097 vs. TAIR 10
Match: AT5G04290.1 (kow domain-containing transcription factor 1 )

HSP 1 Score: 657.1 bits (1694), Expect = 3.3e-188
Identity = 630/1634 (38.56%), Postives = 842/1634 (51.53%), Query Frame = 0

Query: 1    MASKGKG---IAKDTSSGKRKQREDNNGAD---LARKRRDRSVLQFFEDVAP--EVGGES 60
            M  KGKG      D+ SG +K++      D     +KR++  VLQFFE+ A     GG S
Sbjct: 1    MDRKGKGKQVAGSDSYSGGQKRKNSVEFRDEGLRIKKRKNPEVLQFFEESAEVGYYGGSS 60

Query: 61   DDSD----FLDDFMEEEFDTEPAFK-NDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQG 120
            D+ D    FL+D ME+E + E + K     K ++   FPKEE++NEEEFDRIMEE Y  G
Sbjct: 61   DEDDDGLGFLND-MEDEPEVEESSKAGKGEKGKSSFVFPKEEDLNEEEFDRIMEERYKPG 120

Query: 121  PGLGAFAEENYENKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFG 180
             G   +A+++   K++   + L P+S+D   +WKVKC +GRER+SVFCLM KFV+L   G
Sbjct: 121  SGFLRYADDDI--KDAIEMDALAPTSKDP-PIWKVKCAIGRERRSVFCLMHKFVELRKIG 180

Query: 181  TKLQIKSAFYVDHVKGFIYIEAPRQYDLIEACKGISGIYSTRIASVPENDISQLLTVRSR 240
            TKL+I S F VDHVKGFI+IEA +++D++EACK + GIY+TR+  +P+ +   LLTV+ +
Sbjct: 181  TKLEIISVFSVDHVKGFIFIEADKEHDVLEACKSLVGIYATRMVLLPKAETPNLLTVQKK 240

Query: 241  VSEVSVGTMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGVAAKKT 300
              +VS GT ARVKNGKYKGDLAQIVAV++ R +A +KL+PRID+QA+  K+GGGV  +K 
Sbjct: 241  TKKVSEGTWARVKNGKYKGDLAQIVAVSDTRNKALIKLIPRIDIQALTQKYGGGVTVQKG 300

Query: 301  TNPAPRLINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVM 360
              PAPRLI+SSEL+EFRPL+Q RRDR+TG  FE LD +MLKDGYL+KK+SLDS++ WGV+
Sbjct: 301  QTPAPRLISSSELEEFRPLIQVRRDRDTGITFEHLDSLMLKDGYLYKKVSLDSISSWGVI 360

Query: 361  PSEDELLKFKPPESNESNDLEWLSQLYGEKRKKKIIRTEKGGGKGEGTSGS--------- 420
            P++DELLKF P +  E+ D+EW+S++YGE+RKKKI+ T + GGKGEG+ G          
Sbjct: 361  PTKDELLKFTPVDRKETGDVEWISEIYGEERKKKILPTCREGGKGEGSGGGKGEGSGGGK 420

Query: 421  ---------------SSMNSFADHDLVCFGRKDFGMILGT-EKDDSYKILKEGPDGSIVV 480
                            S +S+  ++LVCF RKDFG+I+G  +K D YK+LKEG DG +VV
Sbjct: 421  GEGSRGGKGEGSSDFKSESSYELYNLVCFSRKDFGLIVGVDDKGDGYKVLKEGIDGPVVV 480

Query: 481  NVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVFVYDEN 540
             V +KE+++GP + KFTA D N K ISV+D VK+ +G  + KQG+V+ VYR  +F+YDE+
Sbjct: 481  TVGKKEMQNGPFDSKFTALDLNKKQISVNDVVKISKGPSEGKQGVVRQVYRGIIFLYDES 540

Query: 541  EVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWAEKDSG 600
            E +N GYFCCKS  CEK+K+  +  + K      + FEDF SSPKSPLSP+K W  ++  
Sbjct: 541  EEENGGYFCCKSQSCEKVKLFTEESNEKTGGFDGTAFEDFVSSPKSPLSPEKEWQPRERY 600

Query: 601  REYNRDDKDGMFSIGQTLRIRVGPLKGYLCRVIAVRKKDVTVKLDSQQKVLTVRSDFLSE 660
               N+ D    +SIGQ LRIRVGPLKGYLCRVIA+R  DVTVKLDSQ K+ TV+S+ L+E
Sbjct: 601  NSSNQGDIGSTYSIGQKLRIRVGPLKGYLCRVIALRYSDVTVKLDSQHKIFTVKSEHLAE 660

Query: 661  VYRKT---STVEDTEFGSLKPFDILGNEGSSQDWMGGTGSSTGGDGWNSAGPSSERSPWP 720
            V  +    ST  D   GS +PF +LG E S+ DW  G G+S+ G  WN  GPS++     
Sbjct: 661  VRDRNTVLSTSGDAGTGSFQPFGMLGTESSTGDWAIGAGTSSEGGNWNIGGPSTD----- 720

Query: 721  SFPESGTSVSFFIPSSSLSSFYVASMIITDVEDSPWVSKTVPNASTSWGAAK--SNVDDT 780
                           S  S     +M+    E +P            WG +K  S+V  T
Sbjct: 721  ---------------SHESLNIERNMVQLCREKNP------------WGGSKPTSDVSPT 780

Query: 781  VNDDQAPGWGKSESWDKATAKTSSDGNASGAWDKKVVPGGDYKDSESG---GWKKSQNAS 840
            V DD    W  +     A  K +S  +  G W+    P G    SE+G   GW       
Sbjct: 781  VADDNTSAWANA----AAENKPASASDQPGGWN----PWGKTPASEAGTVSGW------- 840

Query: 841  FGDENNSAEASANKWSSKNRSSGGWGDCNASTTVSEIKPAGKSNAGSSAWNISDENSSW- 900
               + +++   A+ W  +  S+    D  +  T          + GSS  N  DE+S W 
Sbjct: 841  --GDTSASNVEASSWEKQGASTSNVADLGSWGT----------HGGSSGGNKQDEDSVWG 900

Query: 901  -----NTQKQESNRSSWGKPSDRGGTRSSESGRGGN----QVRGGSFG-QDSSSD----- 960
                 +   Q+   SSWGK     G  SS   + GN    +  G S+G QD  SD     
Sbjct: 901  KLCEASESSQKKEESSWGKKGGSDG-ESSWGNKDGNSSASKKDGVSWGQQDKGSDESKGG 960

Query: 961  --WNKSTTTDGAGKK----DGWNKPNLASEDESIGKKGWGQGNEASDSGNKWQSSGSDGG 1020
              W+      G+GKK     GWNK    S   S G   WGQ N+ S  G K      DG 
Sbjct: 961  SAWSNQCGDFGSGKKKDGSSGWNKSAEDSNANSKGVPDWGQPNDGSSWGKK-----GDGA 1020

Query: 1021 KKWGTNESERDGGNKSWNTSKSSDGDTGGGNSAIWKDKSDSSSLTAPKGDQWGGDWDKQH 1080
              WG  +   DGG  SW   K  DG+   G S+ W  K D         D  G  W+K+ 
Sbjct: 1021 ASWGKKD---DGG--SW--GKKDDGNKDDGGSS-WGKKDDGQK------DDGGSSWEKKF 1080

Query: 1081 SSNDT--KASEDNSPWNKKSVESGKDGERKDRGSGWNVGKSSGGDSASGWGQTSKEAGSS 1140
                +  K  +  S W KK       G++ D GS W  GK   G S   WG+  K+ G S
Sbjct: 1081 DGGSSWGKKDDGGSSWGKKDDGGSLWGKKDDGGSSW--GKEDDGGSL--WGK--KDDGES 1140

Query: 1141 DQAGSWGSNWKKNSNAGNEDSSWAEK----SNWNSGNESNDNQFTGDTSGHGSWGGDGSD 1200
                SWG   KK+      +SSW +K    S+W   +E   ++ T D  G G  G  G  
Sbjct: 1141 ----SWG---KKDDG----ESSWGKKDDGGSSWGKKDEGGYSEQTFDRGGRGFGGRRGGG 1200

Query: 1201 RGGFR---GRGGFRGRGE---------RGRFGGRGRSDRGGS------DRGG---FGGRG 1260
            R G R   GRG   G  E          G   G+   D GGS      D GG   +G + 
Sbjct: 1201 RRGGRDQFGRGSSFGNSEDPAPWSKPSGGSSWGKQDGDGGGSSWGKENDAGGGSSWGKQD 1260

Query: 1261 RGRWNSEGGSNDGENRGWSSG-----GGGGDWEKSGSDRGGFGGGRGRWSSGGGGSDSGW 1320
             G  +S G  NDG   G S G     GGG  W K   D GG G   G+   GGG S S W
Sbjct: 1261 NGVGSSWGKQNDGSGGGSSWGKQNDAGGGSSWGK--QDSGGDGSSWGK-QDGGGDSGSAW 1320

Query: 1321 GKSGSDRGGFGGGRGRGRWNQGDSNSNDGGNGG--WSSSGGRGGFGGGRGRGRWNQEGDS 1380
            GK  +  GG   G+             DGG GG  W    G GG G   G+      G S
Sbjct: 1321 GKQNNTSGGSSWGKQSDAGGGSSWGKQDGGGGGSSWGKQDGGGGSGSAWGKQNETSNGSS 1380

Query: 1381 SSKDSDNGGWSGGGGGWGSSGSGRGGIGGRGRGRRNQDGGSN------DNEDKPASWSTG 1440
              K +D    SGGG  WG      GG GG   G++N  GG +      D   KP  W+  
Sbjct: 1381 WGKQND----SGGGSSWGKQD---GGGGGSSWGKQNDGGGGSSWGKQGDGGSKP--WNEH 1440

Query: 1441 SGNTGGWSSSGGAGSWSRGGDEKNQQQSWKTNNEGGDQSGGQGSGWKEPSGNEQNTWKS- 1500
            SG   G+    G G + RGG  +NQ     +   G    GG+ S WK  + N++NTWKS 
Sbjct: 1441 SGGGRGFGERRGGGGF-RGG--RNQ-----SGRGGRSFDGGRSSSWK--TDNQENTWKSD 1485

Query: 1501 -SGASGWNKSSAGKDIEESCSQKSSWGQAA--AKTDAQAGGGWGKSASGWNGGSEDQTTK 1520
             SG S W               K  WG+ +  +K    + GG   +   W+  S+ +T  
Sbjct: 1501 QSGGSDW---------------KKGWGEDSNNSKPSGSSAGGCAGNWPSWDTNSKKETND 1485

BLAST of MS010097 vs. TAIR 10
Match: AT4G08350.1 (global transcription factor group A2 )

HSP 1 Score: 275.0 bits (702), Expect = 3.6e-73
Identity = 222/733 (30.29%), Postives = 346/733 (47.20%), Query Frame = 0

Query: 4   KGKGIAKDTSSGKRKQREDNN---------GADLARKRRDRSVLQFFEDVAPEVG--GES 63
           +G+    D  + +  Q ED++         G   A KR+  S   F +  A +V    E 
Sbjct: 45  RGRSNFIDDYAEEDSQEEDDDDEDYGSSRGGKGAASKRKKPSASIFLDREAHQVDDEDEE 104

Query: 64  DDSDFLDDFMEEEFDTEPAFKNDAAKDQNIPFFPKEE-EMNEEEFDRIMEEHYNQGPGLG 123
           ++ +  DDF+ +     P  + D   ++   F P++E + + E+ +R ++E ++      
Sbjct: 105 EEDEAEDDFIVDNGTDLPDERGDRRYERR--FLPRDENDEDVEDLERRIQERFS-----S 164

Query: 124 AFAEENYENKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQ 183
              EE  E      +  L PS RD   LW VKC +GRER+   CLMQKF+D    G  LQ
Sbjct: 165 RHHEEYDEEATEVEQQALLPSVRDP-KLWMVKCAIGREREVAVCLMQKFIDR---GADLQ 224

Query: 184 IKSAFYVDHVKGFIYIEAPRQYDLIEACKGISGIYST-RIASVPENDISQLLTVRSRVSE 243
           I+S   +DH+K FIY+EA ++  + EA KG+  IY+  +I  VP  +++ +L+V S+  +
Sbjct: 225 IRSVVALDHLKNFIYVEADKEAHVKEAIKGMRNIYANQKILLVPIREMTDVLSVESKAID 284

Query: 244 VSVGTMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGG-GVAAKKTTN 303
           +S  T  R+K G YKGDLA++V V+N R+R TVKL+PRIDLQA+A+K  G  V+ KK   
Sbjct: 285 LSRDTWVRMKIGTYKGDLAKVVDVDNVRQRVTVKLIPRIDLQALASKLDGREVSKKKAFV 344

Query: 304 PAPRLINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPS 363
           P PR +N  E  E    ++ RRD  TG  FE + GM+ KDG+ +K++SL S+    V P+
Sbjct: 345 PPPRFMNIDEARELHIRVERRRDHMTGDYFENIGGMLFKDGFHYKQVSLKSITVQNVTPT 404

Query: 364 EDELLKFKPPESNESNDLEWLSQLYGEKRK-----------------------------K 423
            DEL KF  P  N   D   LS L+  ++K                              
Sbjct: 405 FDELEKFNKPSENGEGDFGGLSTLFANRKKGHFMKGDAVIVIKGDLKNLKGWVEKVDEEN 464

Query: 424 KIIRTEKGG--------------------------GKGEG-------------------- 483
            +IR+E  G                          G  EG                    
Sbjct: 465 VLIRSEVKGLPDPLAVNERELCKYFEPGNHVKVVSGTHEGATGMVVKVDQHVLIILSDTT 524

Query: 484 -----------------TSGSSSMNSFADHDLVCFGRKDFGMILGTEKDDSYKILKEGPD 543
                            T+G + +  +  HDLV      FG+I+  E ++++++LK  PD
Sbjct: 525 KEHVRVFADHVVESSEVTTGVTKIGDYELHDLVLLDNLSFGVIIRLE-NEAFQVLKGVPD 584

Query: 544 GSIVVNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVF 603
              V  V+ +E+K   LE K    D    +I+V D+V+V+EG  K KQG VKH+Y+  +F
Sbjct: 585 RPEVALVKLREIKC-KLEKKINVQDRYKNVIAVKDDVRVIEGPSKGKQGPVKHIYKGVLF 644

Query: 604 VYDENEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWA 623
           +YD + +++ G+ C K   C  +  S    +    D   S + +F +    P SP +   
Sbjct: 645 IYDRHHLEHAGFICAKCTSCIVVGGSRSGANRNGGD-SLSRYGNFKAPAPVPSSPGR--F 704

BLAST of MS010097 vs. TAIR 10
Match: AT2G34210.1 (Transcription elongation factor Spt5 )

HSP 1 Score: 241.9 bits (616), Expect = 3.4e-63
Identity = 217/739 (29.36%), Postives = 336/739 (45.47%), Query Frame = 0

Query: 14  SGKRKQR--EDNNGADLARKRRDRSVLQFFE-DVAPEVGGESDDSDFLD--------DF- 73
           SGK++ R   D++G   ++K+   S    +E +V  +V  + DD D  D        DF 
Sbjct: 36  SGKKRGRSNSDSDGRRGSKKKSSGSAFIDWEVEVDDDVEDDDDDVDVEDGKQQLKFGDFS 95

Query: 74  ----MEEEFDTEPAFKNDAAKDQNIPFFPKEEEMNEEEFDRIMEEHYNQGPGLGAFAEEN 133
               +  E D      +   +     F P EE+++E E  R +E    +      +A+++
Sbjct: 96  LCFIVSGEADLPNEDSDHRRQYYQRGFHPHEEDVDELE-KRTLERLSTK------YAKDD 155

Query: 134 YE--NKNSTGRNPLQPSSRDTISLWKVKCMVGRERQSVFCLMQKFVDLHSFGTKLQIKSA 193
           YE  + N   +  L PS RD   LW VKC +GRER+   CLMQK VD    G++ +I+SA
Sbjct: 156 YELDDVNDVDQQALLPSVRDP-KLWLVKCAIGREREVAVCLMQKIVDR---GSEFKIRSA 215

Query: 194 FYVDHVKGFIYIEAPRQYDLIEACKGISGIYST-RIASVPENDISQLLTVRSRVSEVSVG 253
             +DH++ ++YIEA  +  + EA KG+  IY+  +I  VP  +++ +L+V S+  ++S  
Sbjct: 216 IALDHLQNYVYIEADMEAHVKEAIKGMRNIYANQKILLVPIKEMTAVLSVESKAIDLSRD 275

Query: 254 TMARVKNGKYKGDLAQIVAVNNARKRATVKLVPRIDLQAMAAKFGGGV-AAKKTTNPAPR 313
           +  R+K G YKGDLAQ+V V+N RKR TVKL+PRIDLQA+A K  G     KK   P PR
Sbjct: 276 SWVRMKLGIYKGDLAQVVDVDNVRKRVTVKLIPRIDLQALANKLEGTENVKKKAFAPPPR 335

Query: 314 LINSSELDEFRPLMQFRRDRETGKLFEFLDGMMLKDGYLFKKISLDSLNCWGVMPSEDEL 373
            +N  E  E    ++ RRD  TG  FE + GM+ KDG+L+KK+S  S+    V P+ DEL
Sbjct: 336 FMNIDEARELHIRVEHRRDPMTGDYFENIGGMLFKDGFLYKKVSTKSIAAQNVTPTFDEL 395

Query: 374 LKFKPPESNESNDLEWLSQLYGEKRK-----------------------------KKIIR 433
            +FK P  N   D    S L+  ++K                               +IR
Sbjct: 396 ERFKRPNENGEIDFVDESTLFANRKKGHFMKGDAVIVIKGDLKNLKGWIEKVDEENVLIR 455

Query: 434 TE-------------------------------KGGGKG--------------------- 493
           +E                                 GG G                     
Sbjct: 456 SEMKDLPNPIAVNGRELCKYFEPGNFVKVVSGIHEGGTGMIVKVDQHMLIILSDTTKEHI 515

Query: 494 -----------EGTSGSSSMNSFADHDLVCFGRKDFGMILGTEKDDSYKILKEGPDGSIV 553
                      E T G + +  +  HDLV      FG+IL  +  ++ +ILK  PD S V
Sbjct: 516 CVFADHVAKSAEVTKGVTKIGDYELHDLVILSDFSFGVILKLD-SEAIQILKGVPDSSEV 575

Query: 554 VNVQRKELKSGPLEGKFTAADHNGKIISVSDNVKVLEGSLKDKQGIVKHVYRHTVFVYDE 613
             V+  E+K   +  K    D    +++V D V+V+EG  K KQG V  +Y+  +F++D 
Sbjct: 576 SIVKASEIKY-KIWKKINVQDRYKNVVAVKDVVRVIEGPSKGKQGPVVQIYKGVLFIHDR 635

Query: 614 NEVDNDGYFCCKSNMCEKIKISYDAPSGKDDDKGFSGFEDFSSSPKSPLSPKKPWAEKDS 641
           + +++ G+ C + + C     ++  P+            D   +P +             
Sbjct: 636 HNLEHTGFICTRCSSCVLAGGNFKTPALVPPSPRRFQRADMGYNPGA-------GGRHQG 695