MS010087 (gene) Bitter gourd (TR) v1

Overview


Name	MS010087
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	S4 RNA-binding domain-containing protein
Location	scaffold779: 516722 .. 519821 (+)
RNA-Seq Expression	MS010087
Synteny	MS010087

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCCTTAGCCGCAGCACTCTCTTCACTCTCCATTCTCTCCAAACCTTCCTTTATCCTCAACCCCATTCGCGCCATTCCTCGCATTTCCTGCTCTCTCTCCTCCAATTCCGCTCCCAATGCCTCCTCTTCATCTCCGTTGGAGTTCAATATCACATTCGCGCCCCCAAAGCCGAAACCCAAATCCGAAATCCAACACCCATTTCAGTTCATTGATCCAAACACCGGCGGTCAGCTCTTAATTCCCTGGATTGTTCGCGGCGAGGATGGCAACCTCCAGCTCCAATCGCACCCTCCTACGCGGTTCCTCCAATCCATGTCGGAGGCTGAAACCAAGCCCAGGAGGAAGAAGGGTTCTGCCGATAAGCGCGTGCCTGAACCACCCCCCAAGCATTCCAAGGCTGCCCGGAGATTTTACAACGAGAATATTAGGGACTCGTCGCAACGTCTCAGTAAAGTCCTTGCTGCTGCTGGAGGTCGTTCTTCTGACTTTTGATAACGTTTCTGTCTTTTTACTTATTGTTGTTTGGCAGTTGGTGCGGCGCTAGTCGTCTTTCCTTGGGTTATTTTTTGAAAAATGTTTAGTTATACCGATAAGGAGTTCTAGAAATATAATGGAAGGAATATCTACTTGTAGTTTAGTTAGTAATGTGGCGACACGCCTAGATGCAGAAGACGCACTTGTGTTGGTGTAAATCCATGCTTGAGATGACTTTACTTGGCCCGAACTTCAAACGGTCATCCTTTGATATGAGCTCTAACATAGGGTCAATGTTCAACTATACTTCAATCAGAAACAATGTCAAAGGCCCCCAAAGAACCAAGAAAGAAATATGATTGGAGCTCAACATCGTCTATCACGCATCCTTCTTTTGTTCTTGTTGTCTATGCATACTTTTTTATTTTTGCTGCTTAGTAGTTAGTGCTCAGTTACTTGCTGCTTGTTTGGCTTATGTTAAGGGTAATTCTTCTCTTTGAGCTTCATTTTTCTTAAAAGTAGTATGGTGGACTATGTAAATTGACATGTATTGTAAAAGTTCGCTTTCGTGAGCAATGACAGATATGACTAAATATTTCTTGTATTTATCCTGTTTGACCCTTTCGGAGGTGTGAACATTTGGTTTGAGTTTTTGTATTTAGATACGTAGATGGAGGATGTGCCCTATTTTTATCCTGTTTCTCCATCTACTTCATTCATGGTAGGAACTTAAGTTTGAATACTTCTGTGGATAACATGTTATTTCTAGCTAGTCTCAACCAGGTTAAGAAGACTGGGGATGAAAGTTCAGGTGTTCACCATTTGGTTGAGTTTGTAATTATAATTTCCATGAACTAAATGTTAAGTTGTAAGGCTAGGTACATGATCCTTCCTCGTGTTTTATGGTTTAGCATTGGTCTTGGAGTGTTCTGTTTTGTTTTCTTCTCCAATGTCCTGTATACGTTAACTATCATGGTCCCAGTAAGATCTCATAGTTGGTATTGTCTCCTCTTTTGTTTGTCAGTGGCATCAAGACGGAGCAGTGAAGAGCTTATTTTTGAAGGCCGTGTGACTGTTAATGGTTCTGTTTGCAATACTCCTCAGGTCAGTTGGCTGTACCGTGTGGGTTGGATGGATTTTTGTCTTAACCACGAAGGAATAAATGATTCACATTTCTGTAGACTCGGGTGGATCCTGCAAGAGACGTTATTTATGTGAATGGAAATAGGCTCCCCAAGAAGCTGCCGCCAAAAGTTTATCTAGCCTTGAACAAGCCAAAAGGGTTTGTCACTTAACCTCTGCGGTCAATTTTCCTCCCCTACCTGTGATTCCATTGCTAATTTTATTTAATGCTACGGCAGGTATATCTGTTCATCTGGGAAAAAGGAATCGAAGTCTGTGATAAGTTTATTTGATGATTATTTGAAGAGTTGGGTATGTTGAACCTACTAATCCATGTTGTCTTCAAGCAAAAGACTAGAAAGGAGCGGTGATCTTGTGATCATATTTACTCATGAAATATCATTTGCAAGTTATTCAACTAAAACTTTTTATTTGCAGGATAAAACATATCCAGGACAACCCAAACCACGTCTGTTTACTGTTGGCCGACTTGATGTTGCTACAACTGGGTTGATTATAGTGACCAATGATGGTATGTTCTTTGACAAACTAATCATCGTTGCCTCCGTTTTTAGGATTTCCATAGAAAAAATTCGGAGTTAGAAGGACAAAATTAACATTGATATTTCTTTCAGGAGATTTTGCTCAAAGTATCTCACATCCTTCATCTGGCTTATCGAAGGAGTAAGTGATTGCATTTTCTTTCTTAACTATGGCTGAGGTATTATAAGGTTACCTACAAAAATAATTTTTGAAATAATTTCCATGGAGTAATTTAAAGATCAACAGATCAACCAAAAACTCCGACATCATAGTTACCTACTGTGTACTGACCATTTCAGGGATAAGGAATATGATACTGATCCTTTTCTTTTGTTCTTTTTTCCCTATAAAAAATTCAATTAAAGCATTTTCTTTTCTTTTTTTATGCTTCCTAATACATAAATTTTTAAGTTTTTGAATGTTGTGATTACTAAAAATACCAACATCTGCACTTTATAACTGTAGATACATTGCAGCAATAGATGGTGCTGTTAGTAAGCAGAACCTGATAGCAATCAGTGAGGGAACAATAATTGATGGCGTCCATTGCACCCCAGATTCTGTGGAGCTGCTACCGCGACAGCCAGATGTATCAAGATCTCGTCTGCGTATTGTGGTGCGTTAGTCCATCCATATTATACTTTTTGAATCTCTATGCATTCTCAGCTCATTCATGCCTTCTTGTTTGATCTAATTGATCTTGAAGTTATGATTGTAAAGACCTGCAACGTCTCTTGCTTTTCTAACCTGAATGTTGTTGGTATTTATGTTCTCTTCTCTATAATTTTCGATGCTATGCAGTAGTCTGTGTGGAAGAAAATATACTAGTACCTTTTTTCTGCTCCCGATGCTTGTTACAGCTCCGGACTAACTTAATGTGCTTTTGCAGGTACATGAAGGAAGGAACCATGAAGTTCGAGAACTTGTAAAAGCTGCTGGTCTAGAGGTTAGCTTC

mRNA sequence

ATGGCTTCCTTAGCCGCAGCACTCTCTTCACTCTCCATTCTCTCCAAACCTTCCTTTATCCTCAACCCCATTCGCGCCATTCCTCGCATTTCCTGCTCTCTCTCCTCCAATTCCGCTCCCAATGCCTCCTCTTCATCTCCGTTGGAGTTCAATATCACATTCGCGCCCCCAAAGCCGAAACCCAAATCCGAAATCCAACACCCATTTCAGTTCATTGATCCAAACACCGGCGGTCAGCTCTTAATTCCCTGGATTGTTCGCGGCGAGGATGGCAACCTCCAGCTCCAATCGCACCCTCCTACGCGGTTCCTCCAATCCATGTCGGAGGCTGAAACCAAGCCCAGGAGGAAGAAGGGTTCTGCCGATAAGCGCGTGCCTGAACCACCCCCCAAGCATTCCAAGGCTGCCCGGAGATTTTACAACGAGAATATTAGGGACTCGTCGCAACGTCTCAGTAAAGTCCTTGCTGCTGCTGGAGTGGCATCAAGACGGAGCAGTGAAGAGCTTATTTTTGAAGGCCGTGTGACTGTTAATGGTTCTGTTTGCAATACTCCTCAGACTCGGGTGGATCCTGCAAGAGACGTTATTTATGTGAATGGAAATAGGCTCCCCAAGAAGCTGCCGCCAAAAGTTTATCTAGCCTTGAACAAGCCAAAAGGGTATATCTGTTCATCTGGGAAAAAGGAATCGAAGTCTGTGATAAGTTTATTTGATGATTATTTGAAGAGTTGGGATAAAACATATCCAGGACAACCCAAACCACGTCTGTTTACTGTTGGCCGACTTGATGTTGCTACAACTGGGTTGATTATAGTGACCAATGATGGAGATTTTGCTCAAAGTATCTCACATCCTTCATCTGGCTTATCGAAGGAATACATTGCAGCAATAGATGGTGCTGTTAGTAAGCAGAACCTGATAGCAATCAGTGAGGGAACAATAATTGATGGCGTCCATTGCACCCCAGATTCTGTGGAGCTGCTACCGCGACAGCCAGATGTATCAAGATCTCGTCTGCGTATTGTGGTACATGAAGGAAGGAACCATGAAGTTCGAGAACTTGTAAAAGCTGCTGGTCTAGAGGTTAGCTTC

Coding sequence (CDS)

ATGGCTTCCTTAGCCGCAGCACTCTCTTCACTCTCCATTCTCTCCAAACCTTCCTTTATCCTCAACCCCATTCGCGCCATTCCTCGCATTTCCTGCTCTCTCTCCTCCAATTCCGCTCCCAATGCCTCCTCTTCATCTCCGTTGGAGTTCAATATCACATTCGCGCCCCCAAAGCCGAAACCCAAATCCGAAATCCAACACCCATTTCAGTTCATTGATCCAAACACCGGCGGTCAGCTCTTAATTCCCTGGATTGTTCGCGGCGAGGATGGCAACCTCCAGCTCCAATCGCACCCTCCTACGCGGTTCCTCCAATCCATGTCGGAGGCTGAAACCAAGCCCAGGAGGAAGAAGGGTTCTGCCGATAAGCGCGTGCCTGAACCACCCCCCAAGCATTCCAAGGCTGCCCGGAGATTTTACAACGAGAATATTAGGGACTCGTCGCAACGTCTCAGTAAAGTCCTTGCTGCTGCTGGAGTGGCATCAAGACGGAGCAGTGAAGAGCTTATTTTTGAAGGCCGTGTGACTGTTAATGGTTCTGTTTGCAATACTCCTCAGACTCGGGTGGATCCTGCAAGAGACGTTATTTATGTGAATGGAAATAGGCTCCCCAAGAAGCTGCCGCCAAAAGTTTATCTAGCCTTGAACAAGCCAAAAGGGTATATCTGTTCATCTGGGAAAAAGGAATCGAAGTCTGTGATAAGTTTATTTGATGATTATTTGAAGAGTTGGGATAAAACATATCCAGGACAACCCAAACCACGTCTGTTTACTGTTGGCCGACTTGATGTTGCTACAACTGGGTTGATTATAGTGACCAATGATGGAGATTTTGCTCAAAGTATCTCACATCCTTCATCTGGCTTATCGAAGGAATACATTGCAGCAATAGATGGTGCTGTTAGTAAGCAGAACCTGATAGCAATCAGTGAGGGAACAATAATTGATGGCGTCCATTGCACCCCAGATTCTGTGGAGCTGCTACCGCGACAGCCAGATGTATCAAGATCTCGTCTGCGTATTGTGGTACATGAAGGAAGGAACCATGAAGTTCGAGAACTTGTAAAAGCTGCTGGTCTAGAGGTTAGCTTC

Protein sequence

MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGSADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEVSF

Homology

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BLAST of MS010087 vs. NCBI nr
Match: XP_022133495.1 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Momordica charantia])

HSP 1 Score: 706.4 bits (1822), Expect = 1.2e-199
Identity = 361/362 (99.72%), Postives = 362/362 (100.00%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK
Sbjct: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS
Sbjct: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS
Sbjct: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360

Query: 361 EV 363
           E+
Sbjct: 361 EI 362

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BLAST of MS010087 vs. NCBI nr
Match: XP_038884576.1 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Benincasa hispida])

HSP 1 Score: 643.3 bits (1658), Expect = 1.3e-180
Identity = 334/364 (91.76%), Postives = 345/364 (94.78%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNA--SSSSPLEFNITFAPPK 60
           M SLA+  SSLS LSKPSFILNPIRAIPRISCSLSSNS PNA  SSSS LEFNITFAPPK
Sbjct: 1   MVSLAS--SSLSFLSKPSFILNPIRAIPRISCSLSSNSTPNASSSSSSSLEFNITFAPPK 60

Query: 61  PKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKK 120
           PKPKSE++ PFQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSE ETKP++KK
Sbjct: 61  PKPKSEVEGPFQFIDRNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEDETKPKKKK 120

Query: 121 GSADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180
            SADKR P  PPKHSKAARRFYNENIRD+SQRLSKVLAAAGVASRRSSEELIFEGRVTVN
Sbjct: 121 VSADKR-PTEPPKHSKAARRFYNENIRDASQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180

Query: 181 GSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD 240
           GSVCNTPQTRVDPARDVIYVNGNRLPKKLP KVYLALNKPKGYICSSGKKESKSVISLFD
Sbjct: 181 GSVCNTPQTRVDPARDVIYVNGNRLPKKLPQKVYLALNKPKGYICSSGKKESKSVISLFD 240

Query: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAID 300
           DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLI+VTNDGDFAQSISHPSSGLSKEYIAAID
Sbjct: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLILVTNDGDFAQSISHPSSGLSKEYIAAID 300

Query: 301 GAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAA 360
           GAVSKQNLIAISEGTIIDG+HCTPDSVELLPRQPD+SR RLRIVVHEGRNHEVRELVKAA
Sbjct: 301 GAVSKQNLIAISEGTIIDGIHCTPDSVELLPRQPDISRPRLRIVVHEGRNHEVRELVKAA 360

Query: 361 GLEV 363
           GLE+
Sbjct: 361 GLEI 361

[Close]

BLAST of MS010087 vs. NCBI nr
Match: XP_016901969.1 (PREDICTED: ribosomal large subunit pseudouridine synthase B isoform X2 [Cucumis melo])

HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 326/362 (90.06%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           M SLA+  SSLS LSKPS I N IRAIPRISCSLSSNS PNASSSS  EFNITFAPPKPK
Sbjct: 1   MVSLAS--SSLSFLSKPSLIFNRIRAIPRISCSLSSNSTPNASSSSSFEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKS+++ PFQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSE ETKP++KK S
Sbjct: 61  PKSDVEDPFQFIDRNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEDETKPKKKKVS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           A K + E PPKHSKAARRFYNENIR+SSQRLSKVLAAAGVASRRSSEELIF GRVTVNGS
Sbjct: 121 AGKPITE-PPKHSKAARRFYNENIRESSQRLSKVLAAAGVASRRSSEELIFGGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARD+IYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDIIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDG 
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGD 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGT IDGVHCTPDSVELLPRQPD+SR RLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTTIDGVHCTPDSVELLPRQPDISRPRLRIVVHEGRNHEVRELVKAAGL 359

Query: 361 EV 363
           E+
Sbjct: 361 EI 359

[Close]

BLAST of MS010087 vs. NCBI nr
Match: XP_008456519.1 (PREDICTED: uncharacterized RNA pseudouridine synthase aq_1464 isoform X1 [Cucumis melo])

HSP 1 Score: 631.3 bits (1627), Expect = 5.1e-177
Identity = 326/362 (90.06%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           M SLA+  SSLS LSKPS I N IRAIPRISCSLSSNS PNASSSS  EFNITFAPPKPK
Sbjct: 1   MVSLAS--SSLSFLSKPSLIFNRIRAIPRISCSLSSNSTPNASSSSSFEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKS+++ PFQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSE ETKP++KK S
Sbjct: 61  PKSDVEDPFQFIDRNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEDETKPKKKKVS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           A K + E PPKHSKAARRFYNENIR+SSQRLSKVLAAAGVASRRSSEELIF GRVTVNGS
Sbjct: 121 AGKPITE-PPKHSKAARRFYNENIRESSQRLSKVLAAAGVASRRSSEELIFGGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARD+IYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDIIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDG 
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGD 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGT IDGVHCTPDSVELLPRQPD+SR RLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTTIDGVHCTPDSVELLPRQPDISRPRLRIVVHEGRNHEVRELVKAAGL 359

Query: 361 EV 363
           E+
Sbjct: 361 EI 359

[Close]

BLAST of MS010087 vs. NCBI nr
Match: XP_004140075.1 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Cucumis sativus] >KGN48104.2 hypothetical protein Csa_003766 [Cucumis sativus])

HSP 1 Score: 629.4 bits (1622), Expect = 1.9e-176
Identity = 326/364 (89.56%), Postives = 341/364 (93.68%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNA--SSSSPLEFNITFAPPK 60
           M SLA+  SSL +LSKPS I NPIRAIPRISCSLSSNS PNA  SSSS LEFNITFAPPK
Sbjct: 1   MVSLAS--SSLLLLSKPSLIFNPIRAIPRISCSLSSNSKPNASSSSSSSLEFNITFAPPK 60

Query: 61  PKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKK 120
           PKPKSE++ PFQFID N+GGQL IPWIVRGEDGNL+LQSHPPTRFL S+SE ETKP++KK
Sbjct: 61  PKPKSEVEDPFQFIDRNSGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSVSEDETKPKKKK 120

Query: 121 GSADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180
            SA K + E PPKHSKAARRFYNENIR+SSQRLSKVLAAAGVASRRSSEELIF GRVTVN
Sbjct: 121 VSAGKPITE-PPKHSKAARRFYNENIRESSQRLSKVLAAAGVASRRSSEELIFGGRVTVN 180

Query: 181 GSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD 240
           GSVCNTPQTRVDPARD+IYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD
Sbjct: 181 GSVCNTPQTRVDPARDIIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD 240

Query: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAID 300
           DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQ ISHPSSGLSKEYIAAID
Sbjct: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQGISHPSSGLSKEYIAAID 300

Query: 301 GAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAA 360
           G VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPD+S+SRLRIVVHEGRNHEVRELVKAA
Sbjct: 301 GTVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDISKSRLRIVVHEGRNHEVRELVKAA 360

Query: 361 GLEV 363
           GLE+
Sbjct: 361 GLEI 361

[Close]

BLAST of MS010087 vs. ExPASy Swiss-Prot
Match: Q8L960 (Putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SVR1 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.7e-122
Identity = 241/386 (62.44%), Postives = 287/386 (74.35%), Query Frame = 0


Query: 1   MASLAAALS---SLSILSKPSFILNP-IRAIPRISCSLSSNSAPNASSSSPLEFNITFAP 60
           MAS+AA+ S   + S L   +F L+P    I  + CS+SS      SSS P+EF+I+FAP
Sbjct: 1   MASVAASSSISFAASFLKIKAFPLSPRFFPIRTLRCSVSS------SSSEPIEFDISFAP 60

Query: 61  PKPKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMS-EAETKPR 120
           PKPKP S            T  QL IPWIVR +DG L+LQS PP R + +++ +A T+  
Sbjct: 61  PKPKPSST-------HGGVTPQQLFIPWIVRSDDGTLKLQSQPPARLIHNLAIDATTQNP 120

Query: 121 RKKGSADKRVP-------------------EPPPKHSKAARRFYNENIRDSSQRLSKVLA 180
           +KK  + K+ P                   E  PK SKAARRFYNEN ++  QRLSKVLA
Sbjct: 121 KKKDKSKKKQPQATSSSSATTTASSPASHSEVKPKLSKAARRFYNENFKEQPQRLSKVLA 180

Query: 181 AAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALN 240
           AAGVASRR+SEELIF+G+VTVNG +CNTPQTRVDP+RD+IYVNGNR+PKKLPPKVY ALN
Sbjct: 181 AAGVASRRTSEELIFDGKVTVNGILCNTPQTRVDPSRDIIYVNGNRIPKKLPPKVYFALN 240

Query: 241 KPKGYICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDG 300
           KPKGYICSSG+KE KS ISLFD+YL SWDK  PG PKPRLFTVGRLDVATTGLI+VTNDG
Sbjct: 241 KPKGYICSSGEKEIKSAISLFDEYLSSWDKRNPGTPKPRLFTVGRLDVATTGLIVVTNDG 300

Query: 301 DFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSR 360
           DFAQ +SHPSS L KEYI  + G + K++L+AISEGT+++GVHC PDSVEL+P+Q D+ R
Sbjct: 301 DFAQKLSHPSSSLPKEYITTVVGDIHKRHLMAISEGTMVEGVHCVPDSVELMPKQHDIPR 360

Query: 361 SRLRIVVHEGRNHEVRELVKAAGLEV 363
           +RLRIVVHEGRNHEVRELVK AGLEV
Sbjct: 361 ARLRIVVHEGRNHEVRELVKNAGLEV 373

[Close]

BLAST of MS010087 vs. ExPASy Swiss-Prot
Match: P35159 (Ribosomal large subunit pseudouridine synthase B OS=Bacillus subtilis (strain 168) OX=224308 GN=rluB PE=1 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 2.2e-29
Identity = 78/214 (36.45%), Postives = 121/214 (56.54%), Query Frame = 0


Query: 149 QRLSKVLAAAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLP 208
           +RL KV+A AGVASRR +EELI EG+V VNG V      +V    D I VNG ++ ++ P
Sbjct: 2   ERLQKVIAHAGVASRRKAEELIKEGKVKVNGKVVTELGVKV-TGSDQIEVNGLKVEREEP 61

Query: 209 PKVYLALNKPKGYICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTG 268
             VY  L KP+G I ++   + + V++   D+ K+  +        R++ +GRLD  T+G
Sbjct: 62  --VYFLLYKPRGVISAAQDDKGRKVVT---DFFKNIPQ--------RIYPIGRLDYDTSG 121

Query: 269 LIIVTNDGDFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELL 328
           L+++TNDG+FA  + HP   + K Y+A + G   K+ L  +  G  ++     P   +LL
Sbjct: 122 LLLLTNDGEFANKLMHPKYEIDKTYVAKVKGIPPKELLRKLERGIRLEEGKTAPAKAKLL 181

Query: 329 PRQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEV 363
                   S +++ +HEGRN +VR + +A G EV
Sbjct: 182 SLDKKKQTSIIQLTIHEGRNRQVRRMFEAIGHEV 201

[Close]

BLAST of MS010087 vs. ExPASy Swiss-Prot
Match: O67444 (Uncharacterized RNA pseudouridine synthase aq_1464 OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1464 PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.4e-28
Identity = 79/215 (36.74%), Postives = 118/215 (54.88%), Query Frame = 0


Query: 150 RLSKVLAAAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPP 209
           R++K L+ AGVASRR +E+LI EGRV VNG V  +   +V+P  D++ V+G   P K   
Sbjct: 5   RINKFLSEAGVASRRKAEKLILEGRVKVNGEVVRSLGVKVNPEVDIVEVDGK--PVKPQR 64

Query: 210 KVYLALNKPKGYICSSGKKES--KSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATT 269
           K Y+ LNKP  Y+   G+     K++  L  D               R+F VGRLD  T 
Sbjct: 65  KRYIILNKPCCYLTQLGRSPDGRKTIEELIKDI------------PERVFPVGRLDYNTE 124

Query: 270 GLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVEL 329
           GL+I+TNDG+ A  I HP   L K Y+A ++G V ++ L  + +G  ++     PD++ +
Sbjct: 125 GLLILTNDGELANRILHPRYKLPKVYLALVEGKVDQKTLKRMKQGIELEDGFAKPDNIRI 184

Query: 330 LPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEV 363
           +  + +   + L I  HEGR H V+  + A G +V
Sbjct: 185 V--RYEGKNTLLEITFHEGRKHLVKRFLGAFGHKV 203

[Close]

BLAST of MS010087 vs. ExPASy Swiss-Prot
Match: O05668 (Uncharacterized RNA pseudouridine synthase ML1370 OS=Mycobacterium leprae (strain TN) OX=272631 GN=ML1370 PE=3 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 4.1e-28
Identity = 83/214 (38.79%), Postives = 119/214 (55.61%), Query Frame = 0


Query: 150 RLSKVLAAAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPP 209
           RL K+L+ AG+ASRR++E+LI EGRV V+G +     TRVDP   V+ V+G ++      
Sbjct: 17  RLQKILSRAGIASRRAAEKLIIEGRVEVDGQLVRELGTRVDPDVSVVRVDGVKVVVD-DS 76

Query: 210 KVYLALNKPKG-YICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTG 269
            VYLALNKP+G Y   S  +    V  L +  ++            +LF VGRLD  T G
Sbjct: 77  LVYLALNKPRGMYSTMSDDRGRPCVGDLIERRVRG---------NKKLFHVGRLDADTEG 136

Query: 270 LIIVTNDGDFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELL 329
           LI++TNDG+ A  + HPS  +SK Y+A + GAV +     +S G  +D      D   ++
Sbjct: 137 LILLTNDGELAHRLMHPSHEVSKTYLATVKGAVPRGLGKKLSVGLELDDGPAHVDDFAVV 196

Query: 330 PRQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEV 363
              P   ++ +R+ +HEGR   VR L+ AAG  V
Sbjct: 197 DAIP--GKTLVRLTLHEGRKRIVRRLLTAAGFPV 218

[Close]

BLAST of MS010087 vs. ExPASy Swiss-Prot
Match: P9WHQ0 (Uncharacterized RNA pseudouridine synthase MT1751.1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1751.1 PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.7e-27
Identity = 81/213 (38.03%), Postives = 118/213 (55.40%), Query Frame = 0


Query: 150 RLSKVLAAAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPP 209
           RL KVL+ AG+ASRR++E++I +GRV V+G V     TRVDP   V+ V+G R+      
Sbjct: 15  RLQKVLSQAGIASRRAAEKMIVDGRVEVDGHVVTELGTRVDPQVAVVRVDGARVVLD-DS 74

Query: 210 KVYLALNKPKGYICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTGL 269
            VYLALNKP+G   +      +  I    D +   ++   G  K  LF VGRLD  T GL
Sbjct: 75  LVYLALNKPRGMHSTMSDDRGRPCIG---DLI---ERKVRGTKK--LFHVGRLDADTEGL 134

Query: 270 IIVTNDGDFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELLP 329
           +++TNDG+ A  + HPS  + K Y+A + G+V +     +  G  +D      D   ++ 
Sbjct: 135 MLLTNDGELAHRLMHPSHEVPKTYLATVTGSVPRGLGRTLRAGIELDDGPAFVDDFAVVD 194

Query: 330 RQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEV 363
             P   ++ +R+ +HEGRN  VR L+ AAG  V
Sbjct: 195 AIP--GKTLVRVTLHEGRNRIVRRLLAAAGFPV 216

[Close]

BLAST of MS010087 vs. ExPASy TrEMBL
Match: A0A6J1BVE6 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006060 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.0e-200
Identity = 361/362 (99.72%), Postives = 362/362 (100.00%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK
Sbjct: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS
Sbjct: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS
Sbjct: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360

Query: 361 EV 363
           E+
Sbjct: 361 EI 362

[Close]

BLAST of MS010087 vs. ExPASy TrEMBL
Match: A0A1S3C3I9 (uncharacterized RNA pseudouridine synthase aq_1464 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496448 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.4e-177
Identity = 326/362 (90.06%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           M SLA+  SSLS LSKPS I N IRAIPRISCSLSSNS PNASSSS  EFNITFAPPKPK
Sbjct: 1   MVSLAS--SSLSFLSKPSLIFNRIRAIPRISCSLSSNSTPNASSSSSFEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKS+++ PFQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSE ETKP++KK S
Sbjct: 61  PKSDVEDPFQFIDRNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEDETKPKKKKVS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           A K + E PPKHSKAARRFYNENIR+SSQRLSKVLAAAGVASRRSSEELIF GRVTVNGS
Sbjct: 121 AGKPITE-PPKHSKAARRFYNENIRESSQRLSKVLAAAGVASRRSSEELIFGGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARD+IYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDIIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDG 
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGD 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGT IDGVHCTPDSVELLPRQPD+SR RLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTTIDGVHCTPDSVELLPRQPDISRPRLRIVVHEGRNHEVRELVKAAGL 359

Query: 361 EV 363
           E+
Sbjct: 361 EI 359

[Close]

BLAST of MS010087 vs. ExPASy TrEMBL
Match: A0A1S4E166 (ribosomal large subunit pseudouridine synthase B isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496448 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.4e-177
Identity = 326/362 (90.06%), Postives = 338/362 (93.37%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPNASSSSPLEFNITFAPPKPK 60
           M SLA+  SSLS LSKPS I N IRAIPRISCSLSSNS PNASSSS  EFNITFAPPKPK
Sbjct: 1   MVSLAS--SSLSFLSKPSLIFNRIRAIPRISCSLSSNSTPNASSSSSFEFNITFAPPKPK 60

Query: 61  PKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKKGS 120
           PKS+++ PFQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSE ETKP++KK S
Sbjct: 61  PKSDVEDPFQFIDRNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEDETKPKKKKVS 120

Query: 121 ADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVNGS 180
           A K + E PPKHSKAARRFYNENIR+SSQRLSKVLAAAGVASRRSSEELIF GRVTVNGS
Sbjct: 121 AGKPITE-PPKHSKAARRFYNENIRESSQRLSKVLAAAGVASRRSSEELIFGGRVTVNGS 180

Query: 181 VCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240
           VCNTPQTRVDPARD+IYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY
Sbjct: 181 VCNTPQTRVDPARDIIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFDDY 240

Query: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGA 300
           LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDG 
Sbjct: 241 LKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAIDGD 300

Query: 301 VSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGL 360
           VSKQNLIAISEGT IDGVHCTPDSVELLPRQPD+SR RLRIVVHEGRNHEVRELVKAAGL
Sbjct: 301 VSKQNLIAISEGTTIDGVHCTPDSVELLPRQPDISRPRLRIVVHEGRNHEVRELVKAAGL 359

Query: 361 EV 363
           E+
Sbjct: 361 EI 359

[Close]

BLAST of MS010087 vs. ExPASy TrEMBL
Match: A0A6J1GVH4 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111457913 PE=3 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.1e-174
Identity = 323/364 (88.74%), Postives = 338/364 (92.86%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPN--ASSSSPLEFNITFAPPK 60
           M SLAA  SSLS+LSKPS ILNPIRAI RISCSLSSNSAPN  +SSSS LEFNITFAPP 
Sbjct: 1   MVSLAA--SSLSVLSKPSLILNPIRAISRISCSLSSNSAPNGSSSSSSSLEFNITFAPPT 60

Query: 61  PKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKK 120
           PKPKS+    FQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSEAE KP++KK
Sbjct: 61  PKPKSKTDDSFQFIDQNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEAEAKPKKKK 120

Query: 121 GSADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180
            S DKRVPE PPK+SKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN
Sbjct: 121 VSVDKRVPE-PPKYSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180

Query: 181 GSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD 240
           GSVCNTPQTRVDPARD+IYVNGNRLPKKLP KVYLALNKPKGYICSSGKKESKSVI+LFD
Sbjct: 181 GSVCNTPQTRVDPARDIIYVNGNRLPKKLPSKVYLALNKPKGYICSSGKKESKSVINLFD 240

Query: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAID 300
           DYLKSW KTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSG+SKEYIAAID
Sbjct: 241 DYLKSWHKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGISKEYIAAID 300

Query: 301 GAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAA 360
           GAVSKQNLI ISEGT+IDGVHC PD+VELLP+QPD+ RSRLRI VHEGRNHEVRELVKAA
Sbjct: 301 GAVSKQNLITISEGTVIDGVHCIPDAVELLPQQPDIPRSRLRITVHEGRNHEVRELVKAA 360

Query: 361 GLEV 363
           GLE+
Sbjct: 361 GLEI 361

[Close]

BLAST of MS010087 vs. ExPASy TrEMBL
Match: A0A6J1JT41 (putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487252 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 1.9e-174
Identity = 322/364 (88.46%), Postives = 338/364 (92.86%), Query Frame = 0


Query: 1   MASLAAALSSLSILSKPSFILNPIRAIPRISCSLSSNSAPN--ASSSSPLEFNITFAPPK 60
           M SLAA  SSLS+LSKPS ILNPIRAI RISCSLSSNSAPN  +SSSS +EFNITFAPP 
Sbjct: 1   MVSLAA--SSLSVLSKPSLILNPIRAISRISCSLSSNSAPNGSSSSSSSMEFNITFAPPT 60

Query: 61  PKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMSEAETKPRRKK 120
           PKPKS+    FQFID NTGGQL IPWIVRGEDGNL+LQSHPPTRFL SMSEAE KP++KK
Sbjct: 61  PKPKSKTDDSFQFIDQNTGGQLFIPWIVRGEDGNLKLQSHPPTRFLHSMSEAEAKPKKKK 120

Query: 121 GSADKRVPEPPPKHSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180
            S DKRVPE PPK+SKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN
Sbjct: 121 VSVDKRVPE-PPKYSKAARRFYNENIRDSSQRLSKVLAAAGVASRRSSEELIFEGRVTVN 180

Query: 181 GSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALNKPKGYICSSGKKESKSVISLFD 240
           GSVCNTPQTRVDPARD+IYVNGNRLPKKLP KVYLALNKPKGYICSSGKKESKSVI+LFD
Sbjct: 181 GSVCNTPQTRVDPARDIIYVNGNRLPKKLPSKVYLALNKPKGYICSSGKKESKSVINLFD 240

Query: 241 DYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGLSKEYIAAID 300
           DYLKSW KTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSG+SKEYIAAID
Sbjct: 241 DYLKSWHKTYPGQPKPRLFTVGRLDVATTGLIIVTNDGDFAQSISHPSSGISKEYIAAID 300

Query: 301 GAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAA 360
           GAVSKQNLI ISEGT+IDGVHC PD+VELLP+QPD+ RSRLRI VHEGRNHEVRELVKAA
Sbjct: 301 GAVSKQNLITISEGTVIDGVHCIPDAVELLPQQPDIPRSRLRITVHEGRNHEVRELVKAA 360

Query: 361 GLEV 363
           GLE+
Sbjct: 361 GLEI 361

[Close]

BLAST of MS010087 vs. TAIR 10
Match: AT2G39140.1 (pseudouridine synthase family protein )

HSP 1 Score: 440.7 bits (1132), Expect = 1.2e-123
Identity = 241/386 (62.44%), Postives = 287/386 (74.35%), Query Frame = 0


Query: 1   MASLAAALS---SLSILSKPSFILNP-IRAIPRISCSLSSNSAPNASSSSPLEFNITFAP 60
           MAS+AA+ S   + S L   +F L+P    I  + CS+SS      SSS P+EF+I+FAP
Sbjct: 1   MASVAASSSISFAASFLKIKAFPLSPRFFPIRTLRCSVSS------SSSEPIEFDISFAP 60

Query: 61  PKPKPKSEIQHPFQFIDPNTGGQLLIPWIVRGEDGNLQLQSHPPTRFLQSMS-EAETKPR 120
           PKPKP S            T  QL IPWIVR +DG L+LQS PP R + +++ +A T+  
Sbjct: 61  PKPKPSST-------HGGVTPQQLFIPWIVRSDDGTLKLQSQPPARLIHNLAIDATTQNP 120

Query: 121 RKKGSADKRVP-------------------EPPPKHSKAARRFYNENIRDSSQRLSKVLA 180
           +KK  + K+ P                   E  PK SKAARRFYNEN ++  QRLSKVLA
Sbjct: 121 KKKDKSKKKQPQATSSSSATTTASSPASHSEVKPKLSKAARRFYNENFKEQPQRLSKVLA 180

Query: 181 AAGVASRRSSEELIFEGRVTVNGSVCNTPQTRVDPARDVIYVNGNRLPKKLPPKVYLALN 240
           AAGVASRR+SEELIF+G+VTVNG +CNTPQTRVDP+RD+IYVNGNR+PKKLPPKVY ALN
Sbjct: 181 AAGVASRRTSEELIFDGKVTVNGILCNTPQTRVDPSRDIIYVNGNRIPKKLPPKVYFALN 240

Query: 241 KPKGYICSSGKKESKSVISLFDDYLKSWDKTYPGQPKPRLFTVGRLDVATTGLIIVTNDG 300
           KPKGYICSSG+KE KS ISLFD+YL SWDK  PG PKPRLFTVGRLDVATTGLI+VTNDG
Sbjct: 241 KPKGYICSSGEKEIKSAISLFDEYLSSWDKRNPGTPKPRLFTVGRLDVATTGLIVVTNDG 300

Query: 301 DFAQSISHPSSGLSKEYIAAIDGAVSKQNLIAISEGTIIDGVHCTPDSVELLPRQPDVSR 360
           DFAQ +SHPSS L KEYI  + G + K++L+AISEGT+++GVHC PDSVEL+P+Q D+ R
Sbjct: 301 DFAQKLSHPSSSLPKEYITTVVGDIHKRHLMAISEGTMVEGVHCVPDSVELMPKQHDIPR 360

Query: 361 SRLRIVVHEGRNHEVRELVKAAGLEV 363
           +RLRIVVHEGRNHEVRELVK AGLEV
Sbjct: 361 ARLRIVVHEGRNHEVRELVKNAGLEV 373

[Close]

BLAST of MS010087 vs. TAIR 10
Match: AT3G43340.1 (Pseudouridine synthase family protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.1e-17
Identity = 40/56 (71.43%), Postives = 49/56 (87.50%), Query Frame = 0


Query: 307 IAISEGTIIDGVHCTPDSVELLPRQPDVSRSRLRIVVHEGRNHEVRELVKAAGLEV 363
           +AISEGT+++G+HC PD VEL+P+Q D+ R+ LRIVVHEGRNHEVRELVK  GLEV
Sbjct: 1   MAISEGTMVEGIHCVPDLVELMPKQHDIPRAWLRIVVHEGRNHEVRELVKNVGLEV 56

The following BLAST results are available for this feature:

BLAST of MS010087 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133495.1	1.2e-199	99.72	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Mom...	[more]
XP_038884576.1	1.3e-180	91.76	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Ben...	[more]
XP_016901969.1	5.1e-177	90.06	PREDICTED: ribosomal large subunit pseudouridine synthase B isoform X2 [Cucumis ...	[more]
XP_008456519.1	5.1e-177	90.06	PREDICTED: uncharacterized RNA pseudouridine synthase aq_1464 isoform X1 [Cucumi...	[more]
XP_004140075.1	1.9e-176	89.56	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic [Cuc...	[more]

BLAST of MS010087 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L960	1.7e-122	62.44	Putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=A...	[more]
P35159	2.2e-29	36.45	Ribosomal large subunit pseudouridine synthase B OS=Bacillus subtilis (strain 16...	[more]
O67444	2.4e-28	36.74	Uncharacterized RNA pseudouridine synthase aq_1464 OS=Aquifex aeolicus (strain V...	[more]
O05668	4.1e-28	38.79	Uncharacterized RNA pseudouridine synthase ML1370 OS=Mycobacterium leprae (strai...	[more]
P9WHQ0	7.7e-27	38.03	Uncharacterized RNA pseudouridine synthase MT1751.1 OS=Mycobacterium tuberculosi...	[more]

BLAST of MS010087 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVE6	6.0e-200	99.72	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=M...	[more]
A0A1S3C3I9	2.4e-177	90.06	uncharacterized RNA pseudouridine synthase aq_1464 isoform X1 OS=Cucumis melo OX...	[more]
A0A1S4E166	2.4e-177	90.06	ribosomal large subunit pseudouridine synthase B isoform X2 OS=Cucumis melo OX=3...	[more]
A0A6J1GVH4	1.1e-174	88.74	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=C...	[more]
A0A6J1JT41	1.9e-174	88.46	putative ribosomal large subunit pseudouridine synthase SVR1, chloroplastic OS=C...	[more]

BLAST of MS010087 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39140.1	1.2e-123	62.44	pseudouridine synthase family protein	[more]
AT3G43340.1	1.1e-17	71.43	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002942	RNA-binding S4 domain	SMART	SM00363	s4_6	coord: 149..208 e-value: 2.8E-14 score: 63.4
IPR002942	RNA-binding S4 domain	PFAM	PF01479	S4	coord: 149..191 e-value: 3.2E-11 score: 42.8
IPR002942	RNA-binding S4 domain	CDD	cd00165	S4	coord: 149..216 e-value: 6.99835E-11 score: 55.7186
IPR020094	Pseudouridine synthase TruA/RsuA/RluB/E/F, N-terminal	GENE3D	3.30.70.580		coord: 212..288 e-value: 2.0E-37 score: 130.3
IPR036986	RNA-binding S4 domain superfamily	GENE3D	3.10.290.10		coord: 145..205 e-value: 4.8E-19 score: 69.4
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 212..354 e-value: 1.3E-16 score: 61.1
IPR042092	Pseudouridine synthase, RsuA/RluB/E/F, catalytic domain	GENE3D	3.30.70.1560		coord: 289..363 e-value: 2.0E-37 score: 130.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 95..136
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 109..136
None	No IPR available	PANTHER	PTHR47683	PSEUDOURIDINE SYNTHASE FAMILY PROTEIN-RELATED	coord: 38..362
None	No IPR available	PROSITE	PS50889	S4	coord: 149..218 score: 14.38512
None	No IPR available	SUPERFAMILY	55174	Alpha-L RNA-binding motif	coord: 124..231
IPR018496	Pseudouridine synthase, RsuA/RluB/E/F, conserved site	PROSITE	PS01149	PSI_RSU	coord: 260..274
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 211..362

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010087.1	MS010087.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0016866	intramolecular transferase activity
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding