MS010076 (gene) Bitter gourd (TR) v1

BLAST of MS010076 vs. NCBI nr
Match: XP_022134403.1 (synaptotagmin-3-like isoform X1 [Momordica charantia])

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 544/547 (99.45%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI
Sbjct: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           P+WVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS
Sbjct: 61  PVWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW
Sbjct: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
           AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSI GLYQFVQETIKKQIANLYL
Sbjct: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSISGLYQFVQETIKKQIANLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHVFGIPILDSSIAM KKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 241 WPHVFGIPILDSSIAMTKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK
Sbjct: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL
Sbjct: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420

Query: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480
           DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML
Sbjct: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480

Query: 481 EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV 540
           EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV
Sbjct: 481 EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV 540

Query: 541 EIQWTVA 548
           EIQWTVA
Sbjct: 541 EIQWTVA 547

BLAST of MS010076 vs. NCBI nr
Match: XP_038884155.1 (synaptotagmin-3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 961.8 bits (2485), Expect = 2.5e-276
Identity = 476/548 (86.86%), Postives = 506/548 (92.34%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LGIIGFG IG+PLGL+VGFFLFIYS+P EVKDPMIRPIYELDS+ALEELIPEI
Sbjct: 1   MGFFSTLLGIIGFG-IGIPLGLVVGFFLFIYSKPDEVKDPMIRPIYELDSDALEELIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDWLNKFLAAMWPYLDKAIC RIR +AKPMFA YIGKF IQSIEFE LS
Sbjct: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICCRIRGMAKPMFAVYIGKFQIQSIEFENLS 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP+LYG KVHETNENEIVMETA+RWAGNPNIVL+LRLFSL I+IQLVDLQIF AP 
Sbjct: 121 LGTLPPKLYGIKVHETNENEIVMETAIRWAGNPNIVLMLRLFSLQIRIQLVDLQIFVAPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPLVP FPCFS IVVSLLEKPHVDFGMK+ G D+MSIPGL QFVQE I+KQ++NLYL
Sbjct: 181 VALKPLVPAFPCFSKIVVSLLEKPHVDFGMKIQGGDIMSIPGLSQFVQEIIRKQVSNLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHVF IPILD+S+A  KKPVGILHV VV+ALKLSKMD+LGTSDPYVKLS+SGERLPSKK
Sbjct: 241 WPHVFEIPILDASVAATKKPVGILHVNVVKALKLSKMDILGTSDPYVKLSISGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNP+WNEKFKLIVKDP+SQVLQLQVYDWDKVGGHDRLGMQLVPLKVL PYE K
Sbjct: 301 TTIKMNNLNPIWNEKFKLIVKDPESQVLQLQVYDWDKVGGHDRLGMQLVPLKVLTPYEKK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDLLKNTNIND  NKKPRGQLVVEL FTPFREESSK S  LD  CRSM SQ+ RDL 
Sbjct: 361 ELTLDLLKNTNINDHQNKKPRGQLVVELKFTPFREESSKFSSRLD-GCRSMVSQDERDLH 420

Query: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480
           DD VGGAGLLSVK+QGAT VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWNEDFQFML
Sbjct: 421 DDVVGGAGLLSVKIQGATSVEGKRHSNPYAVMHFRGEKKKTKMVKKCRDPVWNEDFQFML 480

Query: 481 EEPPLEEKVHIEVRSRR-TLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIH 540
           EEPPLEEK+HIEVRSRR ++FSFLSKESLGHVEINL DVVHNGRIN KYHLINS++GMIH
Sbjct: 481 EEPPLEEKIHIEVRSRRSSIFSFLSKESLGHVEINLVDVVHNGRINTKYHLINSRYGMIH 540

Query: 541 VEIQWTVA 548
           VEIQWTVA
Sbjct: 541 VEIQWTVA 546

BLAST of MS010076 vs. NCBI nr
Match: XP_008456493.1 (PREDICTED: synaptotagmin-3 isoform X1 [Cucumis melo])

HSP 1 Score: 956.8 bits (2472), Expect = 7.9e-275
Identity = 466/547 (85.19%), Postives = 505/547 (92.32%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LGIIGFG IG+PLGL++GFF FIYS+P EVKDPMIRPIYELDS+ALEE+IPEI
Sbjct: 1   MGFFSTLLGIIGFG-IGIPLGLVIGFFFFIYSKPDEVKDPMIRPIYELDSDALEEVIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDWLNKF+AAMWPYLDKAIC  IR ++KPMFAEYIGKF IQSIEFE L 
Sbjct: 61  PLWVKHPDFDRVDWLNKFIAAMWPYLDKAICSSIRGMSKPMFAEYIGKFQIQSIEFESLL 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP+LYG KVH+TNENEIVMETA++WAGNPNIVL+LRLFSL I+IQLVDLQIFAAP 
Sbjct: 121 LGTLPPKLYGIKVHDTNENEIVMETAIKWAGNPNIVLILRLFSLQIRIQLVDLQIFAAPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPLVP FPCF+NIVVSLLEKPH+DFGMK+LG D+MSIPGLYQFVQETI+KQ++NLYL
Sbjct: 181 VALKPLVPAFPCFANIVVSLLEKPHIDFGMKILGGDIMSIPGLYQFVQETIRKQVSNLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHVF IPILD+S+A  KKPVGILHV VV+A KLSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 241 WPHVFEIPILDASVAATKKPVGILHVNVVKASKLSKMDLLGTSDPYVKLSLSGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNP+WNEKFKL+VKDP+SQVLQLQVYDWDKVGGHDRLGMQLVPLKVL PYETK
Sbjct: 301 TTIKMNNLNPIWNEKFKLVVKDPESQVLQLQVYDWDKVGGHDRLGMQLVPLKVLTPYETK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDLLKNTNIND  NKKPRGQLVVEL FTPFREESSK S  LD  CRSM S++ RDL 
Sbjct: 361 ELTLDLLKNTNINDHQNKKPRGQLVVELKFTPFREESSKFSSQLD-GCRSMVSRDERDLQ 420

Query: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480
           DDFVGGAGLLSVK+QGA  VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWNE F+FML
Sbjct: 421 DDFVGGAGLLSVKIQGAASVEGKRHSNPYAVMHFRGEKKKTKMVKKCRDPVWNETFEFML 480

Query: 481 EEPPLEEKVHIEVRSRR-TLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIH 540
           EEPPLEEK+HIEVRSR+ + FSF SKESLGHVEINL DVVHNGRIN KYHLINS+HGMIH
Sbjct: 481 EEPPLEEKIHIEVRSRKSSRFSFFSKESLGHVEINLGDVVHNGRINTKYHLINSRHGMIH 540

Query: 541 VEIQWTV 547
           VEI+WTV
Sbjct: 541 VEIKWTV 545

BLAST of MS010076 vs. NCBI nr
Match: XP_022134404.1 (synaptotagmin-3-like isoform X2 [Momordica charantia] >XP_022134405.1 synaptotagmin-3-like isoform X2 [Momordica charantia] >XP_022134406.1 synaptotagmin-3-like isoform X2 [Momordica charantia])

HSP 1 Score: 941.4 bits (2432), Expect = 3.4e-270
Identity = 464/466 (99.57%), Postives = 464/466 (99.57%), Query Frame = 0

Query: 82  MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI 141
           MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI
Sbjct: 1   MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI 60

Query: 142 VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL 201
           VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL
Sbjct: 61  VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL 120

Query: 202 EKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAMAKKPV 261
           EKPHVDFGMKVLGCDVMSI GLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAM KKPV
Sbjct: 121 EKPHVDFGMKVLGCDVMSISGLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAMTKKPV 180

Query: 262 GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK 321
           GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK
Sbjct: 181 GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK 240

Query: 322 DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR 381
           DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR
Sbjct: 241 DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR 300

Query: 382 GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE 441
           GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE
Sbjct: 301 GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE 360

Query: 442 GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS 501
           GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS
Sbjct: 361 GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS 420

Query: 502 FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 548
           FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA
Sbjct: 421 FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 466

BLAST of MS010076 vs. NCBI nr
Match: KAG6578767.1 (Synaptotagmin-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 934.9 bits (2415), Expect = 3.2e-268
Identity = 455/549 (82.88%), Postives = 505/549 (91.99%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFF+T+LGIIGFG IG+PLGL++GFFLFI+S+P +VKDPMIRPIYELDS+ALEELIPEI
Sbjct: 12  MGFFNTLLGIIGFG-IGIPLGLIMGFFLFIFSKPDKVKDPMIRPIYELDSDALEELIPEI 71

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDW NKFLAAMWPYLDKAIC  IRA+ KP+F+EYIG F IQSIEFE LS
Sbjct: 72  PLWVKHPDFDRVDWFNKFLAAMWPYLDKAICPSIRAMTKPIFSEYIGTFRIQSIEFESLS 131

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP+LYGFKVHET+ENE++META+RWAGNP++VLVLRLFSL I+IQL+DLQIFAAP 
Sbjct: 132 LGTLPPKLYGFKVHETDENELIMETAIRWAGNPDVVLVLRLFSLQIRIQLIDLQIFAAPR 191

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP FPCFSNIVVSLLEKPHVDFG+K+LG D+MSIPGL+QFVQE I+KQ+ NLYL
Sbjct: 192 IVLKPLVPAFPCFSNIVVSLLEKPHVDFGLKILGGDIMSIPGLHQFVQEAIRKQVLNLYL 251

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP VF IPILD+S+   KKPVGILHV VV+AL+LSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 252 WPRVFEIPILDASVGATKKPVGILHVNVVKALRLSKMDLLGTSDPYVKLSLSGERLPSKK 311

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNPVWNEKFKL+VKDP+SQVL+L+VYDWDKVGGHDRLGMQLVPLKVL P+E K
Sbjct: 312 TTIKMNNLNPVWNEKFKLVVKDPESQVLRLRVYDWDKVGGHDRLGMQLVPLKVLAPHEKK 371

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDL+KNT+IN+  NKKPRGQLVVELTFTPFR ESSK S  LD  CRSM SQN RD+ 
Sbjct: 372 ELTLDLIKNTDINNHQNKKPRGQLVVELTFTPFRVESSKSSSRLD-GCRSMVSQNERDIH 431

Query: 421 DDFV-GGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFM 480
           DDFV GGAGLL VK+QGAT VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWN+DFQFM
Sbjct: 432 DDFVGGGAGLLLVKIQGATSVEGKRHSNPYAVTHFRGEKKKTKMVKKCRDPVWNDDFQFM 491

Query: 481 LEEPPLEEKVHIEVRSRRT-LFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMI 540
           LEEPPL+EK+HIE+RSRR+ LF FLSKESLGHVEINL DVVHNGRIN+KYHLINS+HGMI
Sbjct: 492 LEEPPLKEKIHIELRSRRSGLFGFLSKESLGHVEINLIDVVHNGRINQKYHLINSRHGMI 551

Query: 541 HVEIQWTVA 548
           HVEIQWT+A
Sbjct: 552 HVEIQWTLA 558

BLAST of MS010076 vs. ExPASy Swiss-Prot
Match: Q7XA06 (Synaptotagmin-3 OS=Arabidopsis thaliana OX=3702 GN=SYT3 PE=2 SV=1)

HSP 1 Score: 713.0 bits (1839), Expect = 2.6e-204
Identity = 347/549 (63.21%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFF++VLGIIGF +IG+P+GL++GFF+ IYS+P   + P  RP+ E     L +L+P+I
Sbjct: 1   MGFFTSVLGIIGF-VIGIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLLDLLPDI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLW+K+PD++RVDW NKF++ MWPYLDKA+C  IR+  +P+FA+YIG F I+SIEFE LS
Sbjct: 61  PLWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLS 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP ++G K +ETNE E++ E +++WAGNPNIVLVL++ SL I++QLVDLQ FA   
Sbjct: 121 LGTLPPTVHGVKFYETNEKELLFEPSIKWAGNPNIVLVLKVLSLRIRVQLVDLQFFAIVR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPL+PTFPCF  +VVSL+EKPHVDFG+KVLG D+MSIPGLY++VQETIK+Q++++Y 
Sbjct: 181 VALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYH 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP V  IPILDSS A  KKPVG+LHV +++A  L K DLLGTSDPYVKLSL+GE+LP+KK
Sbjct: 241 WPQVLEIPILDSSTASVKKPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIK  NLNP WNE FKLIVKDP SQVLQL+V+DWDKVGGHDRLGMQ++PL+ + P E K
Sbjct: 301 TTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERK 360

Query: 361 ELTLDLLKNTN-INDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDL 420
           E  LDL+KN+N + DS +KK RG+L V+L + PFREES K          S   +++ D 
Sbjct: 361 EFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFREESIKRRK------ESREEKSSED- 420

Query: 421 LDDFVGGAGLLSVKVQGATGVEG-KRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQF 480
            DDF+  AGLLSV VQ A  VEG K+HSNPY V  FRGEK+KTKM+KK RDP WNE+FQF
Sbjct: 421 -DDFLSQAGLLSVAVQSAKDVEGKKKHSNPYAVVLFRGEKKKTKMLKKTRDPRWNEEFQF 480

Query: 481 MLEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMI 540
            LEEPP++E + +EV S+ T F F SKE LGHV+INL DVV NGRIN+KYHLINS++G+I
Sbjct: 481 TLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDINLDDVVDNGRINQKYHLINSRNGII 540

Query: 541 HVEIQWTVA 548
           H+EI+WT +
Sbjct: 541 HIEIRWTTS 540

BLAST of MS010076 vs. ExPASy Swiss-Prot
Match: B6ETT4 (Synaptotagmin-2 OS=Arabidopsis thaliana OX=3702 GN=SYT2 PE=2 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 5.3e-173
Identity = 296/545 (54.31%), Postives = 402/545 (73.76%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MG  ST+LG+IGFG  G  +G+++G++LFIY +  +V+DP I+P+ ELDSE +  + PEI
Sbjct: 1   MGIISTILGVIGFG-FGTTIGIVIGYYLFIYFQSTDVEDPEIKPLVELDSETIATMFPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           P+WVK+PDFDR+DWLNK +  MWPY+DKAIC   ++IAKP+ AE I  + I S+EFE L+
Sbjct: 61  PMWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T++ EI+ME +V+WAGNPNI++V + F L   +Q++DLQ++A P 
Sbjct: 121 LGSLPPSFQGMKVYATDDKEIIMELSVKWAGNPNIIVVAKAFGLKATVQVIDLQVYATPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP+FPCF+NI VSL++KP VDFG+K+LG DVM+IPGLY+FVQE IK Q+AN+YL
Sbjct: 181 ITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMAIPGLYRFVQEIIKDQVANMYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP    + I+D S AM KKPVG+L VKV++A+KL K DLLG SDPYVKL+LSG+++P KK
Sbjct: 241 WPKTLNVQIMDPSKAM-KKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           T +K +NLNP WNE+F L+VK+P+SQ LQL VYDW++VG HD++GM ++ LK L P E K
Sbjct: 301 TVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPEEPK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
            +TL+LLK+    +  ++K RGQLVVE+ + PF++         D    ++   NA +  
Sbjct: 361 LMTLELLKSMEPKEPVSEKSRGQLVVEVEYKPFKD---------DDIPENIDDPNAVEKA 420

Query: 421 DDFV-GGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFM 480
            +      GLL V V  A  +EGK H+NP +   FRGE+RKTK VKK R+P W+EDFQF 
Sbjct: 421 PEGTPSTGGLLVVIVHEAEDLEGKYHTNPSVRLLFRGEERKTKRVKKNREPRWDEDFQFP 480

Query: 481 LEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIH 540
           L+EPP+ +K+H+EV S  +      KE+LG+V INL DVV N RIN+KYHLI+SK+G I 
Sbjct: 481 LDEPPINDKLHVEVISSSSRL-IHPKETLGYVVINLGDVVSNRRINDKYHLIDSKNGRIQ 533

Query: 541 VEIQW 545
           +E+QW
Sbjct: 541 IELQW 533

BLAST of MS010076 vs. ExPASy Swiss-Prot
Match: Q9SKR2 (Synaptotagmin-1 OS=Arabidopsis thaliana OX=3702 GN=SYT1 PE=1 SV=2)

HSP 1 Score: 594.0 bits (1530), Expect = 1.8e-168
Identity = 292/550 (53.09%), Postives = 394/550 (71.64%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LG  GFG +G+ LGL++G+ LF+Y  P +VKDP IR I + D +A+  ++PEI
Sbjct: 1   MGFFSTILGFCGFG-VGISLGLVIGYVLFVYLLPNDVKDPEIRSIADQDPKAMLRMLPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVK+PDFDRVDW+N+FL  MWPYLDKAIC   + IAKP+  E I K+ I S+EFE L+
Sbjct: 61  PLWVKNPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T+E E++ME  ++WA NPNI++ ++ F L   +Q+VDLQ+FA P 
Sbjct: 121 LGSLPPTFQGMKVYLTDEKELIMEPCLKWAANPNILVAIKAFGLKATVQVVDLQVFAQPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP+FPCF+NI VSL+EKPHVDFG+K+ G D+MSIPGLY+FVQE IK Q+AN+YL
Sbjct: 181 ITLKPLVPSFPCFANIYVSLMEKPHVDFGLKLGGADLMSIPGLYRFVQEQIKDQVANMYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP    +PILD + A  ++PVGI+HVKVV+A+ L K DL+G +DP+VK+ LS +++PSKK
Sbjct: 241 WPKTLVVPILDPAKAF-RRPVGIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TT+K  NLNP WNE+FK  V+DPQ+QVL+  VYDW++VG  +++GM ++ LK + P E K
Sbjct: 301 TTVKHKNLNPEWNEEFKFSVRDPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDEHK 360

Query: 361 ELTLDLLKNTNINDSHN--KKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARD 420
             TL+L K  +  +      K RG+L VEL + PF EE     G  +      A +    
Sbjct: 361 AFTLELRKTLDGGEDGQPPDKYRGKLEVELLYKPFTEEEMP-KGFEETQAVQKAPEGT-- 420

Query: 421 LLDDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQF 480
                    G+L V V  A  VEGK H+NPY+  +F+GE+RKTK VKK RDP WNE+F F
Sbjct: 421 -----PAAGGMLVVIVHSAEDVEGKHHTNPYVRIYFKGEERKTKHVKKNRDPRWNEEFTF 480

Query: 481 MLEEPPLEEKVHIEVRSRRTLFSFL-SKESLGHVEINLTDVVHNGRINEKYHLINSKHGM 540
           MLEEPP+ EK+H+EV S  +    L  KE+LG+V+I + DVV+N R+N+K+HLI+SK+G 
Sbjct: 481 MLEEPPVREKLHVEVLSTSSRIGLLHPKETLGYVDIPVVDVVNNKRMNQKFHLIDSKNGK 540

Query: 541 IHVEIQWTVA 548
           I +E++W  A
Sbjct: 541 IQIELEWRTA 540

BLAST of MS010076 vs. ExPASy Swiss-Prot
Match: Q8L706 (Synaptotagmin-5 OS=Arabidopsis thaliana OX=3702 GN=SYT5 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.9e-70
Identity = 177/562 (31.49%), Postives = 300/562 (53.38%), Query Frame = 0

Query: 11  IGFGIIGVPLGLLVGF-----FLFIYSEPQEVKDPMIRPIYELDSEALEE----LIPEI- 70
           +GF I+GV +GLLVG      F+ + +   +++  +   +       +E+    L PE  
Sbjct: 1   MGF-IVGVVIGLLVGIAIIIGFVKLENSRSKLRSELANTVAAFARMTVEDSRKLLPPEFY 60

Query: 71  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 130
           P WV   +  ++ WLN  L  +WPY+D+A  + I+A  +P+  +Y     + S+ F  L+
Sbjct: 61  PSWVVFSERQKLTWLNHHLTKIWPYVDEAASELIKASVEPVLEQYRPAI-VASLTFSKLT 120

Query: 131 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVL-VLRLFSLNIKIQLVDLQIFAAP 190
           LGT+ P+  G  V + ++N I +E  ++W GNPNIVL V  L  +++ IQ+ ++      
Sbjct: 121 LGTVAPQFTGVSVIDGDKNGITLELDMQWDGNPNIVLGVKTLVGVSLPIQVKNIGFTGVF 180

Query: 191 WAALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLY 250
               +PLV  FPCF  + VSL EK  +DF +KV+G D+ +IPGL + ++ETI+  + +  
Sbjct: 181 RLIFRPLVEDFPCFGAVSVSLREKKKLDFTLKVVGGDISAIPGLSEAIEETIRDAVEDSI 240

Query: 251 LWPHVFGIPILDSSIA-MAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPS 310
            WP    IPI+    + +  KPVG+L VK+VQA  L+  DL+G SDP+ K+ +   R  +
Sbjct: 241 TWPVRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKT 300

Query: 311 KKTTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYE 370
           K++    N+LNP+WNE F+ +V+D  +Q L +++YD + V   + +G   + L  LEP +
Sbjct: 301 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 360

Query: 371 TKELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARD 430
            K++ L L+K+  I    + K RG++ +EL + P+   +   +  +  +  S+      D
Sbjct: 361 VKDVWLKLVKDLEI--QRDTKNRGEVHLELLYIPYGSGNGIVNPFVTSSMTSLERVLKND 420

Query: 431 LLDDFVGGA---------GLLSVKVQGATGVE-----GKRHSNPYIVAHFR--GEKRKTK 490
             D+    +         G+LSV V  A  +      GK  ++PY+V   +  G K KT+
Sbjct: 421 TTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGK--ADPYVVLSMKKSGAKSKTR 480

Query: 491 MVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNG 545
           +V    +PVWN+ F F++E+  L + + +EV    T      K+ +G   + LT V+   
Sbjct: 481 VVNDSLNPVWNQTFDFVVED-GLHDMLVLEVWDHDT----FGKDYIGRCILTLTRVIMEE 540

BLAST of MS010076 vs. ExPASy Swiss-Prot
Match: A0JJX5 (Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.5e-63
Identity = 171/560 (30.54%), Postives = 280/560 (50.00%), Query Frame = 0

Query: 16  IGVPLGLLVGF--FLFIYSEPQEVKDPMIRPIYELDSEALEELIPE--IPLWVKHPDFDR 75
           I V  GL+V F  +  + S  +      I     +  +   +L+P    P WV      +
Sbjct: 11  IAVSFGLVVAFARYSSVRSTRRADLAKTIAAFARMTVQDSRKLLPGDFYPSWVVFSQRQK 70

Query: 76  VDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGF 135
           ++WLN  L  +WPY+++A  + I++  +P+  +Y     + S++F   +LGT+ P+  G 
Sbjct: 71  LNWLNLELEKIWPYVNEAASELIKSSVEPVLEQYTPAM-LASLKFSKFTLGTVAPQFTGV 130

Query: 136 KV--HETNENEIVMETAVRWAGNPNIVL-VLRLFSLNIKIQLVDLQIFAAPWAALKPLVP 195
            +   E+  N I ME  ++W GNP IVL V  L  +++ I++ ++          KPLV 
Sbjct: 131 SILESESGPNGITMELEMQWDGNPKIVLDVKTLLGVSLPIEVKNIGFTGVFRLIFKPLVD 190

Query: 196 TFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYLWPHVFGIP 255
            FPCF  +  SL EK  +DF +KV+G ++ SIPG+   ++ETI+  I +   WP    IP
Sbjct: 191 EFPCFGALSYSLREKKGLDFTLKVIGGELTSIPGISDAIEETIRDAIEDSITWPVRKIIP 250

Query: 256 ILDSSIA-MAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNN 315
           IL    + +  KPVG L VKVVQA  L+  D++G SDPY  + +      +KKT    N+
Sbjct: 251 ILPGDYSDLELKPVGKLDVKVVQAKDLANKDMIGKSDPYAIVFIRPLPDRTKKTKTISNS 310

Query: 316 LNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLL 375
           LNP+WNE F+ IV+D  +Q L ++V+D + VG    +G   VPL  L P + K++ L L+
Sbjct: 311 LNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGKVKDIWLKLV 370

Query: 376 KNTNINDSHNKKPRGQLVVELTFTPFREE----------------------SSKCSGMLD 435
           K+  I    + K RGQ+ +EL + P  +E                       S+ S   D
Sbjct: 371 KDLEI--QRDTKNRGQVQLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPESEDSDATD 430

Query: 436 VTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVK 495
           +  + + +   +D++   V    +++ +   A    GK  +   I       K KT++V 
Sbjct: 431 M--KKLVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSKTRVVP 490

Query: 496 KCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRIN 546
              +PVWN+ F F++E+  L + + +EV           K+ +G V + LT V+  G   
Sbjct: 491 DSLNPVWNQTFDFVVED-ALHDLLTLEVWDH----DKFGKDKIGRVIMTLTRVMLEGEFQ 550

BLAST of MS010076 vs. ExPASy TrEMBL
Match: A0A6J1C1W5 (synaptotagmin-3-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111006671 PE=3 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 544/547 (99.45%), Postives = 545/547 (99.63%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI
Sbjct: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           P+WVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS
Sbjct: 61  PVWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW
Sbjct: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
           AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSI GLYQFVQETIKKQIANLYL
Sbjct: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSISGLYQFVQETIKKQIANLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHVFGIPILDSSIAM KKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 241 WPHVFGIPILDSSIAMTKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK
Sbjct: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL
Sbjct: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420

Query: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480
           DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML
Sbjct: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480

Query: 481 EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV 540
           EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV
Sbjct: 481 EEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHV 540

Query: 541 EIQWTVA 548
           EIQWTVA
Sbjct: 541 EIQWTVA 547

BLAST of MS010076 vs. ExPASy TrEMBL
Match: A0A1S3C2Z0 (synaptotagmin-3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496432 PE=3 SV=1)

HSP 1 Score: 956.8 bits (2472), Expect = 3.8e-275
Identity = 466/547 (85.19%), Postives = 505/547 (92.32%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LGIIGFG IG+PLGL++GFF FIYS+P EVKDPMIRPIYELDS+ALEE+IPEI
Sbjct: 1   MGFFSTLLGIIGFG-IGIPLGLVIGFFFFIYSKPDEVKDPMIRPIYELDSDALEEVIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDWLNKF+AAMWPYLDKAIC  IR ++KPMFAEYIGKF IQSIEFE L 
Sbjct: 61  PLWVKHPDFDRVDWLNKFIAAMWPYLDKAICSSIRGMSKPMFAEYIGKFQIQSIEFESLL 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP+LYG KVH+TNENEIVMETA++WAGNPNIVL+LRLFSL I+IQLVDLQIFAAP 
Sbjct: 121 LGTLPPKLYGIKVHDTNENEIVMETAIKWAGNPNIVLILRLFSLQIRIQLVDLQIFAAPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPLVP FPCF+NIVVSLLEKPH+DFGMK+LG D+MSIPGLYQFVQETI+KQ++NLYL
Sbjct: 181 VALKPLVPAFPCFANIVVSLLEKPHIDFGMKILGGDIMSIPGLYQFVQETIRKQVSNLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHVF IPILD+S+A  KKPVGILHV VV+A KLSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 241 WPHVFEIPILDASVAATKKPVGILHVNVVKASKLSKMDLLGTSDPYVKLSLSGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNP+WNEKFKL+VKDP+SQVLQLQVYDWDKVGGHDRLGMQLVPLKVL PYETK
Sbjct: 301 TTIKMNNLNPIWNEKFKLVVKDPESQVLQLQVYDWDKVGGHDRLGMQLVPLKVLTPYETK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDLLKNTNIND  NKKPRGQLVVEL FTPFREESSK S  LD  CRSM S++ RDL 
Sbjct: 361 ELTLDLLKNTNINDHQNKKPRGQLVVELKFTPFREESSKFSSQLD-GCRSMVSRDERDLQ 420

Query: 421 DDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFML 480
           DDFVGGAGLLSVK+QGA  VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWNE F+FML
Sbjct: 421 DDFVGGAGLLSVKIQGAASVEGKRHSNPYAVMHFRGEKKKTKMVKKCRDPVWNETFEFML 480

Query: 481 EEPPLEEKVHIEVRSRR-TLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIH 540
           EEPPLEEK+HIEVRSR+ + FSF SKESLGHVEINL DVVHNGRIN KYHLINS+HGMIH
Sbjct: 481 EEPPLEEKIHIEVRSRKSSRFSFFSKESLGHVEINLGDVVHNGRINTKYHLINSRHGMIH 540

Query: 541 VEIQWTV 547
           VEI+WTV
Sbjct: 541 VEIKWTV 545

BLAST of MS010076 vs. ExPASy TrEMBL
Match: A0A6J1BXQ8 (synaptotagmin-3-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111006671 PE=3 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 1.7e-270
Identity = 464/466 (99.57%), Postives = 464/466 (99.57%), Query Frame = 0

Query: 82  MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI 141
           MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI
Sbjct: 1   MWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLSLGTLPPRLYGFKVHETNENEI 60

Query: 142 VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL 201
           VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL
Sbjct: 61  VMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPWAALKPLVPTFPCFSNIVVSLL 120

Query: 202 EKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAMAKKPV 261
           EKPHVDFGMKVLGCDVMSI GLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAM KKPV
Sbjct: 121 EKPHVDFGMKVLGCDVMSISGLYQFVQETIKKQIANLYLWPHVFGIPILDSSIAMTKKPV 180

Query: 262 GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK 321
           GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK
Sbjct: 181 GILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVK 240

Query: 322 DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR 381
           DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR
Sbjct: 241 DPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHNKKPR 300

Query: 382 GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE 441
           GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE
Sbjct: 301 GQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVE 360

Query: 442 GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS 501
           GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS
Sbjct: 361 GKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFS 420

Query: 502 FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 548
           FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA
Sbjct: 421 FLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 466

BLAST of MS010076 vs. ExPASy TrEMBL
Match: A0A0A0KE51 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G405950 PE=3 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 2.0e-268
Identity = 460/550 (83.64%), Postives = 500/550 (90.91%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFS ++GI+GFG IG+PLGL+VGFF FIYS+P EVKDPMIRPIYELDS++LEE+IPEI
Sbjct: 1   MGFFSILMGIVGFG-IGIPLGLVVGFFFFIYSKPDEVKDPMIRPIYELDSDSLEEVIPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDWLNKFL  MWP LDKAIC  IR +A+PMFAEYIGKF IQSIEFE L 
Sbjct: 61  PLWVKHPDFDRVDWLNKFLEGMWPCLDKAICSSIRGMAEPMFAEYIGKFQIQSIEFETLL 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTL P+LYG KVHETNENEIVMETA++WAGNPNIVL+LRLFSL I+IQLVDLQIFAAP 
Sbjct: 121 LGTLSPKLYGIKVHETNENEIVMETAIKWAGNPNIVLILRLFSLQIRIQLVDLQIFAAPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPLVP FPCF+NIVVSLLEKPHVDFGMK+LG D+MSIPGLYQFVQETI+KQ++NLYL
Sbjct: 181 VALKPLVPAFPCFTNIVVSLLEKPHVDFGMKILGGDIMSIPGLYQFVQETIRKQVSNLYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WPHV  IPILD+S+A  K+PVGILHV VV+ALKLSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 241 WPHVLEIPILDASVAATKRPVGILHVNVVKALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TT+KMNNLNP+WNEKFKLIVKDP+SQVLQLQVYDWDKVGGHDRLGMQLVPLKVL PYETK
Sbjct: 301 TTVKMNNLNPIWNEKFKLIVKDPESQVLQLQVYDWDKVGGHDRLGMQLVPLKVLTPYETK 360

Query: 361 ELTLDLLK--NTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARD 420
           ELTLDLLK  NTNIN+  NKKPRGQLVVEL FTPFREESSK S  LD  C SM S++ RD
Sbjct: 361 ELTLDLLKNTNTNINEYQNKKPRGQLVVELKFTPFREESSKFSSQLD-GCGSMVSRDERD 420

Query: 421 LLDDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQF 480
           L DD VGGAGLLSVK+QGAT VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWNEDF+F
Sbjct: 421 LQDDCVGGAGLLSVKIQGATSVEGKRHSNPYAVMHFRGEKKKTKMVKKCRDPVWNEDFEF 480

Query: 481 MLEEPPLEEKVHIEVRSRR-TLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGM 540
           MLEEPPL EK+HIEVRSRR + FSF SKESLGHVEINL DVVHNGRIN KYHLINS+HGM
Sbjct: 481 MLEEPPLGEKIHIEVRSRRSSRFSFFSKESLGHVEINLGDVVHNGRINTKYHLINSRHGM 540

Query: 541 IHVEIQWTVA 548
           IHV+I+WTVA
Sbjct: 541 IHVQIKWTVA 548

BLAST of MS010076 vs. ExPASy TrEMBL
Match: A0A6J1FKV8 (synaptotagmin-3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444918 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.7e-267
Identity = 455/547 (83.18%), Postives = 501/547 (91.59%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFF+T+LGIIGFG IG+PLGL +GFFLFI+S+P +VKDPMIRPIYELDS+ALEELIPEI
Sbjct: 12  MGFFNTLLGIIGFG-IGIPLGLFMGFFLFIFSKPDKVKDPMIRPIYELDSDALEELIPEI 71

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVKHPDFDRVDW NKFLAAMWPYLDKAIC  IRA+ KP+FAEYIG F IQSIEFE LS
Sbjct: 72  PLWVKHPDFDRVDWFNKFLAAMWPYLDKAICPSIRAMTKPIFAEYIGTFRIQSIEFESLS 131

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP+LYGFKVHET+ENE+VMETA+RWAGNP++VLVLRLFSL I+IQL+DLQIFAAP 
Sbjct: 132 LGTLPPKLYGFKVHETDENELVMETAIRWAGNPDVVLVLRLFSLQIRIQLIDLQIFAAPR 191

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP FPCFSNIVVSLLEKPHVDFG+K+LG D+MSIPGL+QFVQE I+KQ+ NLYL
Sbjct: 192 IVLKPLVPAFPCFSNIVVSLLEKPHVDFGLKILGGDIMSIPGLHQFVQEAIRKQVLNLYL 251

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP VF IPILD+S+   KKPVGILHV VV+AL+LSKMDLLGTSDPYVKLSLSGERLPSKK
Sbjct: 252 WPRVFEIPILDASVGATKKPVGILHVNVVKALRLSKMDLLGTSDPYVKLSLSGERLPSKK 311

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIKMNNLNPVWNEKFKL+VKDP+SQVL+L+VYDWDKVGGHDRLGMQLVPLKVL P+E K
Sbjct: 312 TTIKMNNLNPVWNEKFKLVVKDPESQVLRLRVYDWDKVGGHDRLGMQLVPLKVLAPHEKK 371

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
           ELTLDL+KNT+IN+  NKKPRGQLVVELTFTPFR ESSK S  LD  CRSM SQN RD+ 
Sbjct: 372 ELTLDLIKNTDINNHQNKKPRGQLVVELTFTPFRVESSKSSSQLD-GCRSMVSQNERDIH 431

Query: 421 DDFV-GGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFM 480
           DDFV GGAGLL VK+QGAT VEGKRHSNPY V HFRGEK+KTKMVKKCRDPVWN+DFQFM
Sbjct: 432 DDFVGGGAGLLLVKIQGATSVEGKRHSNPYAVTHFRGEKKKTKMVKKCRDPVWNDDFQFM 491

Query: 481 LEEPPLEEKVHIEVRSRRT-LFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMI 540
           LEEPPL+EK+HIE+RSRR+  F FLSKESLGHVEINL DVVHNGRIN+KYHLINS+HGMI
Sbjct: 492 LEEPPLKEKIHIELRSRRSGHFGFLSKESLGHVEINLIDVVHNGRINQKYHLINSRHGMI 551

Query: 541 HVEIQWT 546
           HVEIQWT
Sbjct: 552 HVEIQWT 556

BLAST of MS010076 vs. TAIR 10
Match: AT5G04220.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 713.0 bits (1839), Expect = 1.9e-205
Identity = 347/549 (63.21%), Postives = 438/549 (79.78%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFF++VLGIIGF +IG+P+GL++GFF+ IYS+P   + P  RP+ E     L +L+P+I
Sbjct: 1   MGFFTSVLGIIGF-VIGIPIGLILGFFVLIYSQPSHQEYPPARPLVETSISVLLDLLPDI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLW+K+PD++RVDW NKF++ MWPYLDKA+C  IR+  +P+FA+YIG F I+SIEFE LS
Sbjct: 61  PLWMKNPDYERVDWFNKFISYMWPYLDKAVCGIIRSSVQPLFADYIGTFCIESIEFENLS 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LGTLPP ++G K +ETNE E++ E +++WAGNPNIVLVL++ SL I++QLVDLQ FA   
Sbjct: 121 LGTLPPTVHGVKFYETNEKELLFEPSIKWAGNPNIVLVLKVLSLRIRVQLVDLQFFAIVR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
            ALKPL+PTFPCF  +VVSL+EKPHVDFG+KVLG D+MSIPGLY++VQETIK+Q++++Y 
Sbjct: 181 VALKPLLPTFPCFGMVVVSLMEKPHVDFGLKVLGGDLMSIPGLYRYVQETIKRQVSSMYH 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP V  IPILDSS A  KKPVG+LHV +++A  L K DLLGTSDPYVKLSL+GE+LP+KK
Sbjct: 241 WPQVLEIPILDSSTASVKKPVGLLHVSILRARNLLKKDLLGTSDPYVKLSLTGEKLPAKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TTIK  NLNP WNE FKLIVKDP SQVLQL+V+DWDKVGGHDRLGMQ++PL+ + P E K
Sbjct: 301 TTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGERK 360

Query: 361 ELTLDLLKNTN-INDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDL 420
           E  LDL+KN+N + DS +KK RG+L V+L + PFREES K          S   +++ D 
Sbjct: 361 EFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFREESIKRRK------ESREEKSSED- 420

Query: 421 LDDFVGGAGLLSVKVQGATGVEG-KRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQF 480
            DDF+  AGLLSV VQ A  VEG K+HSNPY V  FRGEK+KTKM+KK RDP WNE+FQF
Sbjct: 421 -DDFLSQAGLLSVAVQSAKDVEGKKKHSNPYAVVLFRGEKKKTKMLKKTRDPRWNEEFQF 480

Query: 481 MLEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMI 540
            LEEPP++E + +EV S+ T F F SKE LGHV+INL DVV NGRIN+KYHLINS++G+I
Sbjct: 481 TLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDINLDDVVDNGRINQKYHLINSRNGII 540

Query: 541 HVEIQWTVA 548
           H+EI+WT +
Sbjct: 541 HIEIRWTTS 540

BLAST of MS010076 vs. TAIR 10
Match: AT1G20080.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 609.0 bits (1569), Expect = 3.8e-174
Identity = 296/545 (54.31%), Postives = 402/545 (73.76%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MG  ST+LG+IGFG  G  +G+++G++LFIY +  +V+DP I+P+ ELDSE +  + PEI
Sbjct: 1   MGIISTILGVIGFG-FGTTIGIVIGYYLFIYFQSTDVEDPEIKPLVELDSETIATMFPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           P+WVK+PDFDR+DWLNK +  MWPY+DKAIC   ++IAKP+ AE I  + I S+EFE L+
Sbjct: 61  PMWVKNPDFDRIDWLNKLIGHMWPYMDKAICKMAKSIAKPIIAEQIPNYKIDSVEFEMLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T++ EI+ME +V+WAGNPNI++V + F L   +Q++DLQ++A P 
Sbjct: 121 LGSLPPSFQGMKVYATDDKEIIMELSVKWAGNPNIIVVAKAFGLKATVQVIDLQVYATPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP+FPCF+NI VSL++KP VDFG+K+LG DVM+IPGLY+FVQE IK Q+AN+YL
Sbjct: 181 ITLKPLVPSFPCFANIFVSLMDKPQVDFGLKLLGADVMAIPGLYRFVQEIIKDQVANMYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP    + I+D S AM KKPVG+L VKV++A+KL K DLLG SDPYVKL+LSG+++P KK
Sbjct: 241 WPKTLNVQIMDPSKAM-KKPVGLLSVKVIKAIKLKKKDLLGGSDPYVKLTLSGDKVPGKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           T +K +NLNP WNE+F L+VK+P+SQ LQL VYDW++VG HD++GM ++ LK L P E K
Sbjct: 301 TVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPEEPK 360

Query: 361 ELTLDLLKNTNINDSHNKKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLL 420
            +TL+LLK+    +  ++K RGQLVVE+ + PF++         D    ++   NA +  
Sbjct: 361 LMTLELLKSMEPKEPVSEKSRGQLVVEVEYKPFKD---------DDIPENIDDPNAVEKA 420

Query: 421 DDFV-GGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFM 480
            +      GLL V V  A  +EGK H+NP +   FRGE+RKTK VKK R+P W+EDFQF 
Sbjct: 421 PEGTPSTGGLLVVIVHEAEDLEGKYHTNPSVRLLFRGEERKTKRVKKNREPRWDEDFQFP 480

Query: 481 LEEPPLEEKVHIEVRSRRTLFSFLSKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIH 540
           L+EPP+ +K+H+EV S  +      KE+LG+V INL DVV N RIN+KYHLI+SK+G I 
Sbjct: 481 LDEPPINDKLHVEVISSSSRL-IHPKETLGYVVINLGDVVSNRRINDKYHLIDSKNGRIQ 533

Query: 541 VEIQW 545
           +E+QW
Sbjct: 541 IELQW 533

BLAST of MS010076 vs. TAIR 10
Match: AT2G20990.1 (synaptotagmin A )

HSP 1 Score: 594.0 bits (1530), Expect = 1.3e-169
Identity = 292/550 (53.09%), Postives = 394/550 (71.64%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LG  GFG +G+ LGL++G+ LF+Y  P +VKDP IR I + D +A+  ++PEI
Sbjct: 1   MGFFSTILGFCGFG-VGISLGLVIGYVLFVYLLPNDVKDPEIRSIADQDPKAMLRMLPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVK+PDFDRVDW+N+FL  MWPYLDKAIC   + IAKP+  E I K+ I S+EFE L+
Sbjct: 61  PLWVKNPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T+E E++ME  ++WA NPNI++ ++ F L   +Q+VDLQ+FA P 
Sbjct: 121 LGSLPPTFQGMKVYLTDEKELIMEPCLKWAANPNILVAIKAFGLKATVQVVDLQVFAQPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP+FPCF+NI VSL+EKPHVDFG+K+ G D+MSIPGLY+FVQE IK Q+AN+YL
Sbjct: 181 ITLKPLVPSFPCFANIYVSLMEKPHVDFGLKLGGADLMSIPGLYRFVQEQIKDQVANMYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP    +PILD + A  ++PVGI+HVKVV+A+ L K DL+G +DP+VK+ LS +++PSKK
Sbjct: 241 WPKTLVVPILDPAKAF-RRPVGIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETK 360
           TT+K  NLNP WNE+FK  V+DPQ+QVL+  VYDW++VG  +++GM ++ LK + P E K
Sbjct: 301 TTVKHKNLNPEWNEEFKFSVRDPQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDEHK 360

Query: 361 ELTLDLLKNTNINDSHN--KKPRGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARD 420
             TL+L K  +  +      K RG+L VEL + PF EE     G  +      A +    
Sbjct: 361 AFTLELRKTLDGGEDGQPPDKYRGKLEVELLYKPFTEEEMP-KGFEETQAVQKAPEGT-- 420

Query: 421 LLDDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQF 480
                    G+L V V  A  VEGK H+NPY+  +F+GE+RKTK VKK RDP WNE+F F
Sbjct: 421 -----PAAGGMLVVIVHSAEDVEGKHHTNPYVRIYFKGEERKTKHVKKNRDPRWNEEFTF 480

Query: 481 MLEEPPLEEKVHIEVRSRRTLFSFL-SKESLGHVEINLTDVVHNGRINEKYHLINSKHGM 540
           MLEEPP+ EK+H+EV S  +    L  KE+LG+V+I + DVV+N R+N+K+HLI+SK+G 
Sbjct: 481 MLEEPPVREKLHVEVLSTSSRIGLLHPKETLGYVDIPVVDVVNNKRMNQKFHLIDSKNGK 540

Query: 541 IHVEIQWTVA 548
           I +E++W  A
Sbjct: 541 IQIELEWRTA 540

BLAST of MS010076 vs. TAIR 10
Match: AT2G20990.2 (synaptotagmin A )

HSP 1 Score: 580.5 bits (1495), Expect = 1.4e-165
Identity = 292/574 (50.87%), Postives = 394/574 (68.64%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LG  GFG +G+ LGL++G+ LF+Y  P +VKDP IR I + D +A+  ++PEI
Sbjct: 1   MGFFSTILGFCGFG-VGISLGLVIGYVLFVYLLPNDVKDPEIRSIADQDPKAMLRMLPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVK+PDFDRVDW+N+FL  MWPYLDKAIC   + IAKP+  E I K+ I S+EFE L+
Sbjct: 61  PLWVKNPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T+E E++ME  ++WA NPNI++ ++ F L   +Q+VDLQ+FA P 
Sbjct: 121 LGSLPPTFQGMKVYLTDEKELIMEPCLKWAANPNILVAIKAFGLKATVQVVDLQVFAQPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQETIKKQIANLYL 240
             LKPLVP+FPCF+NI VSL+EKPHVDFG+K+ G D+MSIPGLY+FVQE IK Q+AN+YL
Sbjct: 181 ITLKPLVPSFPCFANIYVSLMEKPHVDFGLKLGGADLMSIPGLYRFVQEQIKDQVANMYL 240

Query: 241 WPHVFGIPILDSSIAMAKKPVGILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKK 300
           WP    +PILD + A  ++PVGI+HVKVV+A+ L K DL+G +DP+VK+ LS +++PSKK
Sbjct: 241 WPKTLVVPILDPAKAF-RRPVGIVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKK 300

Query: 301 TTIKMNNLNPVWNEKFKLIVKDPQSQVLQLQVYDWD------------------------ 360
           TT+K  NLNP WNE+FK  V+DPQ+QVL+  VYDW+                        
Sbjct: 301 TTVKHKNLNPEWNEEFKFSVRDPQTQVLEFSVYDWEQVKSYQKGDWCLGVGIYVIFVFIT 360

Query: 361 KVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHN--KKPRGQLVVELTFTPFR 420
           +VG  +++GM ++ LK + P E K  TL+L K  +  +      K RG+L VEL + PF 
Sbjct: 361 QVGNPEKMGMNVLALKEMVPDEHKAFTLELRKTLDGGEDGQPPDKYRGKLEVELLYKPFT 420

Query: 421 EESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGVEGKRHSNPYIVAHF 480
           EE     G  +      A +             G+L V V  A  VEGK H+NPY+  +F
Sbjct: 421 EEEMP-KGFEETQAVQKAPEGT-------PAAGGMLVVIVHSAEDVEGKHHTNPYVRIYF 480

Query: 481 RGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLFSFL-SKESLGHVEI 540
           +GE+RKTK VKK RDP WNE+F FMLEEPP+ EK+H+EV S  +    L  KE+LG+V+I
Sbjct: 481 KGEERKTKHVKKNRDPRWNEEFTFMLEEPPVREKLHVEVLSTSSRIGLLHPKETLGYVDI 540

Query: 541 NLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 548
            + DVV+N R+N+K+HLI+SK+G I +E++W  A
Sbjct: 541 PVVDVVNNKRMNQKFHLIDSKNGKIQIELEWRTA 564

BLAST of MS010076 vs. TAIR 10
Match: AT2G20990.3 (synaptotagmin A )

HSP 1 Score: 575.1 bits (1481), Expect = 6.0e-164
Identity = 292/588 (49.66%), Postives = 394/588 (67.01%), Query Frame = 0

Query: 1   MGFFSTVLGIIGFGIIGVPLGLLVGFFLFIYSEPQEVKDPMIRPIYELDSEALEELIPEI 60
           MGFFST+LG  GFG +G+ LGL++G+ LF+Y  P +VKDP IR I + D +A+  ++PEI
Sbjct: 1   MGFFSTILGFCGFG-VGISLGLVIGYVLFVYLLPNDVKDPEIRSIADQDPKAMLRMLPEI 60

Query: 61  PLWVKHPDFDRVDWLNKFLAAMWPYLDKAICDRIRAIAKPMFAEYIGKFHIQSIEFECLS 120
           PLWVK+PDFDRVDW+N+FL  MWPYLDKAIC   + IAKP+  E I K+ I S+EFE L+
Sbjct: 61  PLWVKNPDFDRVDWINRFLEYMWPYLDKAICKTAKNIAKPIIEEQIPKYKIDSVEFETLT 120

Query: 121 LGTLPPRLYGFKVHETNENEIVMETAVRWAGNPNIVLVLRLFSLNIKIQLVDLQIFAAPW 180
           LG+LPP   G KV+ T+E E++ME  ++WA NPNI++ ++ F L   +Q+VDLQ+FA P 
Sbjct: 121 LGSLPPTFQGMKVYLTDEKELIMEPCLKWAANPNILVAIKAFGLKATVQVVDLQVFAQPR 180

Query: 181 AALKPLVPTFPCFSNIVVSLLEKPHVDFGMKVLGCDVMSIPGLYQFVQ------------ 240
             LKPLVP+FPCF+NI VSL+EKPHVDFG+K+ G D+MSIPGLY+FVQ            
Sbjct: 181 ITLKPLVPSFPCFANIYVSLMEKPHVDFGLKLGGADLMSIPGLYRFVQVDIFLLHTSFKS 240

Query: 241 --------------------------ETIKKQIANLYLWPHVFGIPILDSSIAMAKKPVG 300
                                     E IK Q+AN+YLWP    +PILD + A  ++PVG
Sbjct: 241 LTCMSKNFFMRERADETVTFRLANTYEQIKDQVANMYLWPKTLVVPILDPAKAF-RRPVG 300

Query: 301 ILHVKVVQALKLSKMDLLGTSDPYVKLSLSGERLPSKKTTIKMNNLNPVWNEKFKLIVKD 360
           I+HVKVV+A+ L K DL+G +DP+VK+ LS +++PSKKTT+K  NLNP WNE+FK  V+D
Sbjct: 301 IVHVKVVRAVGLRKKDLMGGADPFVKIKLSEDKIPSKKTTVKHKNLNPEWNEEFKFSVRD 360

Query: 361 PQSQVLQLQVYDWDKVGGHDRLGMQLVPLKVLEPYETKELTLDLLKNTNINDSHN--KKP 420
           PQ+QVL+  VYDW++VG  +++GM ++ LK + P E K  TL+L K  +  +      K 
Sbjct: 361 PQTQVLEFSVYDWEQVGNPEKMGMNVLALKEMVPDEHKAFTLELRKTLDGGEDGQPPDKY 420

Query: 421 RGQLVVELTFTPFREESSKCSGMLDVTCRSMASQNARDLLDDFVGGAGLLSVKVQGATGV 480
           RG+L VEL + PF EE     G  +      A +             G+L V V  A  V
Sbjct: 421 RGKLEVELLYKPFTEEEMP-KGFEETQAVQKAPEGT-------PAAGGMLVVIVHSAEDV 480

Query: 481 EGKRHSNPYIVAHFRGEKRKTKMVKKCRDPVWNEDFQFMLEEPPLEEKVHIEVRSRRTLF 540
           EGK H+NPY+  +F+GE+RKTK VKK RDP WNE+F FMLEEPP+ EK+H+EV S  +  
Sbjct: 481 EGKHHTNPYVRIYFKGEERKTKHVKKNRDPRWNEEFTFMLEEPPVREKLHVEVLSTSSRI 540

Query: 541 SFL-SKESLGHVEINLTDVVHNGRINEKYHLINSKHGMIHVEIQWTVA 548
             L  KE+LG+V+I + DVV+N R+N+K+HLI+SK+G I +E++W  A
Sbjct: 541 GLLHPKETLGYVDIPVVDVVNNKRMNQKFHLIDSKNGKIQIELEWRTA 578