MS010066 (gene) Bitter gourd (TR) v1

Overview


Name	MS010066
Type	gene
Organism	Momordica charantia cv. TR (Bitter gourd (TR) v1)
Description	Mannan endo-1,4-beta-mannosidase
Location	scaffold779: 336460 .. 339052 (+)
RNA-Seq Expression	MS010066
Synteny	MS010066

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGTCAAAACAATGGGTGCGAGTTTAAAACTAGTTTTTCTGATTGCAGTTTTCATCTCCTCTGTTGAGATTACAGTTTCCACCTCTCTTGAAGGTGATGGGGATAATCAGAAATTTACTGAGGATCATTTCACGTATTCAGACATGAGTCATGGGTAACTATTACTCTTGTCTCCTCACCTCCATTGTTACTCTTGTCTCCTCACCTCAATTCTTCATCTTGTATCGGATTTGATGCATGTGGCCTAAAATATCGTCTACTTTCGCCCTTAAGGGGGAGCCTAGTTGACAAAGGCTTGGGTCTCTTGGTCGTACTGATTTAGAGGTCTCGAGTTTGAACCTTCTAGTGAGCTTAATATCAAAAACTCCTAATGTCTCTCAAGTCTAAATCTTGGGACGAGCACGAGTGCCCCTAGATATAAGAAAACAAAGCTCCGACTCCCAATTACCAAGATATATACATATACAATCTACTTTCATGGCAAATATCAAAACATATTTTTATACAGAATTTTGGGATTGAAATAGTATGAAAGTTAGCCCCAAAATAAGATTTAAACGTAGATTTTTTTGGTTGATCAATATAGGTTGGATCAAGTGGTTCAAAAGGAAGATGATACATGGAGTATGGTGGAGACAAAACAGAACCAATTTGTAGTAGACGGCCAACCGTTCTATGTTAATGGTTTCAACACTTACTGGTTGATGGTATTTGCAGCGGATCAATCCACAAGAGGAAAGGTCACAGAGGTTTTTAAACAAGCTTCTTCTGTGGGTTTAACTGTTTGCAGGACTTGGGCCTTCAATGATGGCCAGTGGAGAGCTCTTCAAAAGTCTCCTTCTGTCTATGATGAAGAAGTCTTCAAGGTACTCATCGGCTATTCATTAGGATTATTTACAGAAACAGCTTCCCATTTTTAAGAACAATAGTAACATCTACAAACCATCCAATCGTAGTTCTTAAAGGTCATTTTGGAAACAAAAATAGAAAATTGTATTCCAACGTATCTACTCATGTAACCATGGTGGCCACTTACTAAGATTTAATATCCTACGAGTTTCCTTATCAACCAAATATAGTAGGATTAGGCGATTGGTCCGTGATTAATCGAGGTGCGCGCAAGCTAGTCTGAACACACATATATCAACAAAAAAAAAAGGAAAAAAATATATATTCACTCATGTTTACTTCTTTGACAAAATTATTGTCGTGCGGTGCATCAGAGATCTATTATCATAATTCCTATTTCATCCATATGTAACTGCCCCTTCTTTTATGAATTGCAGGGATTAGATTTTGTGATTAGTGAGGCCAAGAAGTTTAAGATAAGGCTGATATTATCACTGTCAAACAATTGGGAAGCATATGGTGGCAAAGCACAATATATAAAATGGGGGAGAGCTGCTGGCCTCAATTTGACATCTGATGATGATTTCTTCTCTGATCCAACTCTGAGAAGCTATTACAAGGCTCATGTTAAGGTGAGTTCCAAACGTATATTACTCTGCTTAACATGTTAACTAAATTGAATACAAAATAACCTGAAAATGATATTTGTTGATTTTCATGTGAATGGTATAGACTGTGCTTAACAGAGTGAACACGTTCACAAATGTAACGTACAAGGAGGATCCGACCATTTTTGGCTGGGAATTGATGAACGAACCTCGTTGCACATCAGATCCTTCTGGCGATAAGCTGCAGGTTTGTTGTTCTTTAAGAGGAATTGTATTAGCCCCATAGTTCACAATTCATATCATGAAAAGAAAGATATGTGATTGTGGTGCAACTGAGCAACCAACTTTGTAATTCGAAGCTGTTGAATTGAATGGCATTGGCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATTGATCCAAAGCACTTGGTGGAGGTTGGTTTGGAGGGATTCTATGGCCCATCCACACCCAATAGAGTTCAGTTCAATCCAAATACATATGCCCAACAAGTTGGAACTGATTTCATCAGAAACCATAAGGTTCTTGGTGTTGACTTTGCTTCAGTTCACATTTATGCAGATACATGGTAGGTTCCCAAAACAACATTAGGACCTGAATACTAGAATCTGTTCTTTTCTCATGATATACCTTTGATGATTCTAAACAAGAGTTCATTGTCAAAGGGTTTCTCCGACGATCTCGGACACCCATCTCCAGTTCACAAAATCATGGATGGAGGCTCACATAGAGGATGCAGAGAAGTACCTGGGAATGCCAGTCATTTTTGCCGAGTTTGGTGTATCGTCGAAAGATCCTGGATACAACTCAACTTTCAGGGATGCAGCTCTCAACTTGGTGTACAAGACCCTGTTGGACTCAACAAAGAAAGGAGGGAGTGGGGGAGGAAGCCTGCTGTGGCAGCTGTTCCCTGAAGGCACTGAGTACATGGATGATGGTTATGCAATTGTGCTCTCTAAATCTCCTTCAACTTCAAACATCATATCCCTGCAATCTACAAGGTTGTCCATCTTCAACTCCATTTGTTCCATGAAATGTCGTTGGGGCTGTAAGAAGAAAAATGTTATGGATCTCGACTTTCTCGACCACCATGATCATGATGAGATG

mRNA sequence

GAAGTCAAAACAATGGGTGCGAGTTTAAAACTAGTTTTTCTGATTGCAGTTTTCATCTCCTCTGTTGAGATTACAGTTTCCACCTCTCTTGAAGGTGATGGGGATAATCAGAAATTTACTGAGGATCATTTCACGTATTCAGACATGAGTCATGGGTTGGATCAAGTGGTTCAAAAGGAAGATGATACATGGAGTATGGTGGAGACAAAACAGAACCAATTTGTAGTAGACGGCCAACCGTTCTATGTTAATGGTTTCAACACTTACTGGTTGATGGTATTTGCAGCGGATCAATCCACAAGAGGAAAGGTCACAGAGGTTTTTAAACAAGCTTCTTCTGTGGGTTTAACTGTTTGCAGGACTTGGGCCTTCAATGATGGCCAGTGGAGAGCTCTTCAAAAGTCTCCTTCTGTCTATGATGAAGAAGTCTTCAAGGGATTAGATTTTGTGATTAGTGAGGCCAAGAAGTTTAAGATAAGGCTGATATTATCACTGTCAAACAATTGGGAAGCATATGGTGGCAAAGCACAATATATAAAATGGGGGAGAGCTGCTGGCCTCAATTTGACATCTGATGATGATTTCTTCTCTGATCCAACTCTGAGAAGCTATTACAAGGCTCATGTTAAGACTGTGCTTAACAGAGTGAACACGTTCACAAATGTAACGTACAAGGAGGATCCGACCATTTTTGGCTGGGAATTGATGAACGAACCTCGTTGCACATCAGATCCTTCTGGCGATAAGCTGCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATTGATCCAAAGCACTTGGTGGAGGTTGGTTTGGAGGGATTCTATGGCCCATCCACACCCAATAGAGTTCAGTTCAATCCAAATACATATGCCCAACAAGTTGGAACTGATTTCATCAGAAACCATAAGGTTCTTGGTGTTGACTTTGCTTCAGTTCACATTTATGCAGATACATGGGTTTCTCCGACGATCTCGGACACCCATCTCCAGTTCACAAAATCATGGATGGAGGCTCACATAGAGGATGCAGAGAAGTACCTGGGAATGCCAGTCATTTTTGCCGAGTTTGGTGTATCGTCGAAAGATCCTGGATACAACTCAACTTTCAGGGATGCAGCTCTCAACTTGGTGTACAAGACCCTGTTGGACTCAACAAAGAAAGGAGGGAGTGGGGGAGGAAGCCTGCTGTGGCAGCTGTTCCCTGAAGGCACTGAGTACATGGATGATGGTTATGCAATTGTGCTCTCTAAATCTCCTTCAACTTCAAACATCATATCCCTGCAATCTACAAGGTTGTCCATCTTCAACTCCATTTGTTCCATGAAATGTCGTTGGGGCTGTAAGAAGAAAAATGTTATGGATCTCGACTTTCTCGACCACCATGATCATGATGAGATG

Coding sequence (CDS)

GAAGTCAAAACAATGGGTGCGAGTTTAAAACTAGTTTTTCTGATTGCAGTTTTCATCTCCTCTGTTGAGATTACAGTTTCCACCTCTCTTGAAGGTGATGGGGATAATCAGAAATTTACTGAGGATCATTTCACGTATTCAGACATGAGTCATGGGTTGGATCAAGTGGTTCAAAAGGAAGATGATACATGGAGTATGGTGGAGACAAAACAGAACCAATTTGTAGTAGACGGCCAACCGTTCTATGTTAATGGTTTCAACACTTACTGGTTGATGGTATTTGCAGCGGATCAATCCACAAGAGGAAAGGTCACAGAGGTTTTTAAACAAGCTTCTTCTGTGGGTTTAACTGTTTGCAGGACTTGGGCCTTCAATGATGGCCAGTGGAGAGCTCTTCAAAAGTCTCCTTCTGTCTATGATGAAGAAGTCTTCAAGGGATTAGATTTTGTGATTAGTGAGGCCAAGAAGTTTAAGATAAGGCTGATATTATCACTGTCAAACAATTGGGAAGCATATGGTGGCAAAGCACAATATATAAAATGGGGGAGAGCTGCTGGCCTCAATTTGACATCTGATGATGATTTCTTCTCTGATCCAACTCTGAGAAGCTATTACAAGGCTCATGTTAAGACTGTGCTTAACAGAGTGAACACGTTCACAAATGTAACGTACAAGGAGGATCCGACCATTTTTGGCTGGGAATTGATGAACGAACCTCGTTGCACATCAGATCCTTCTGGCGATAAGCTGCAGGGATGGATTCAAGAAATGGCTGTGTATGTGAAGAGCATTGATCCAAAGCACTTGGTGGAGGTTGGTTTGGAGGGATTCTATGGCCCATCCACACCCAATAGAGTTCAGTTCAATCCAAATACATATGCCCAACAAGTTGGAACTGATTTCATCAGAAACCATAAGGTTCTTGGTGTTGACTTTGCTTCAGTTCACATTTATGCAGATACATGGGTTTCTCCGACGATCTCGGACACCCATCTCCAGTTCACAAAATCATGGATGGAGGCTCACATAGAGGATGCAGAGAAGTACCTGGGAATGCCAGTCATTTTTGCCGAGTTTGGTGTATCGTCGAAAGATCCTGGATACAACTCAACTTTCAGGGATGCAGCTCTCAACTTGGTGTACAAGACCCTGTTGGACTCAACAAAGAAAGGAGGGAGTGGGGGAGGAAGCCTGCTGTGGCAGCTGTTCCCTGAAGGCACTGAGTACATGGATGATGGTTATGCAATTGTGCTCTCTAAATCTCCTTCAACTTCAAACATCATATCCCTGCAATCTACAAGGTTGTCCATCTTCAACTCCATTTGTTCCATGAAATGTCGTTGGGGCTGTAAGAAGAAAAATGTTATGGATCTCGACTTTCTCGACCACCATGATCATGATGAGATG

Protein sequence

EVKTMGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM

Homology

[Close]

BLAST of MS010066 vs. NCBI nr
Match: XP_022134211.1 (mannan endo-1,4-beta-mannosidase 6 isoform X1 [Momordica charantia])

HSP 1 Score: 959.9 bits (2480), Expect = 8.0e-276
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0


Query: 5   MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW 64
           MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW
Sbjct: 1   MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW 60

Query: 65  SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF 124
           SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF
Sbjct: 61  SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF 120

Query: 125 NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA 184
           NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA
Sbjct: 121 NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA 180

Query: 185 AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD 244
           AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD
Sbjct: 181 AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD 240

Query: 245 PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN 304
           PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN
Sbjct: 241 PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN 300

Query: 305 HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK 364
           HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK
Sbjct: 301 HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK 360

Query: 365 DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST 424
           DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST
Sbjct: 361 DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST 420

Query: 425 SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM
Sbjct: 421 SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 465

[Close]

BLAST of MS010066 vs. NCBI nr
Match: XP_022134212.1 (mannan endo-1,4-beta-mannosidase 6 isoform X2 [Momordica charantia])

HSP 1 Score: 879.8 bits (2272), Expect = 1.1e-251
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0


Query: 49  MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF 108
           MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF
Sbjct: 1   MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF 60

Query: 109 KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN 168
           KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN
Sbjct: 61  KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN 120

Query: 169 WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP 228
           WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP
Sbjct: 121 WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP 180

Query: 229 TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF 288
           TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF
Sbjct: 181 TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF 240

Query: 289 NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK 348
           NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK
Sbjct: 241 NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK 300

Query: 349 YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE 408
           YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE
Sbjct: 301 YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE 360

Query: 409 YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE 468
           YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE
Sbjct: 361 YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE 420

Query: 469 M 470
           M
Sbjct: 421 M 421

[Close]

BLAST of MS010066 vs. NCBI nr
Match: XP_038886206.1 (mannan endo-1,4-beta-mannosidase 6 isoform X1 [Benincasa hispida])

HSP 1 Score: 865.9 bits (2236), Expect = 1.6e-247
Identity = 412/469 (87.85%), Postives = 442/469 (94.24%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLEGDGDNQKF-------TEDHFTYSDMSHGLDQVVQKE 67
           SLKL+FLI VF+  VE TVS+ +EGDGD+Q F       TE+HFTYSDMSHG D +VQK+
Sbjct: 9   SLKLIFLIVVFMFCVEYTVSSFVEGDGDDQPFDISVEDATENHFTYSDMSHGFDGLVQKD 68

Query: 68  DDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCR 127
           DD WSMVET+QNQFVVDGQPFYVNGFNTYWLM+FAADQSTRGKVTE+FKQA+SVGLTVCR
Sbjct: 69  DDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTELFKQAASVGLTVCR 128

Query: 128 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIK 187
           TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKK+KIRLILSL+NNWEAYGGKAQY+K
Sbjct: 129 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKYKIRLILSLANNWEAYGGKAQYVK 188

Query: 188 WGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPR 247
           WG+AAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVN+FTNV YKEDPTIF WELMNEPR
Sbjct: 189 WGKAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNSFTNVPYKEDPTIFAWELMNEPR 248

Query: 248 CTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 307
           CTSDPSGDKLQGWIQEMAV+VKS+DPKHL+EVGLEGFYGPSTPNRVQFNPNTYAQQVGTD
Sbjct: 249 CTSDPSGDKLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 308

Query: 308 FIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 367
           FIRNHKVLGVDFASVHIYADTWVS TISDTHLQFTKSWMEAHIEDAEKYLGMPV+FAEFG
Sbjct: 309 FIRNHKVLGVDFASVHIYADTWVSQTISDTHLQFTKSWMEAHIEDAEKYLGMPVLFAEFG 368

Query: 368 VSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSK 427
           VSS+DPGYNST+RD  LN VYKTLLDSTKKGGSGGGSL+WQLFPEGT+YMDDGYAIVLSK
Sbjct: 369 VSSRDPGYNSTYRDTVLNSVYKTLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGYAIVLSK 428

Query: 428 SPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
            PSTSNIISL STR+SIFNSICSM+CRWGCKKKNVMD+ F + +DHDEM
Sbjct: 429 FPSTSNIISLHSTRMSIFNSICSMRCRWGCKKKNVMDIIFPNSYDHDEM 477

[Close]

BLAST of MS010066 vs. NCBI nr
Match: XP_004149910.1 (mannan endo-1,4-beta-mannosidase 6 [Cucumis sativus] >KGN47821.1 hypothetical protein Csa_003284 [Cucumis sativus])

HSP 1 Score: 865.5 bits (2235), Expect = 2.1e-247
Identity = 413/469 (88.06%), Postives = 442/469 (94.24%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLEGDGDNQKF-------TEDHFTYSDMSHGLDQVVQKE 67
           +LKLVFLI V IS VE T+S+ +EG+GD+Q F       TE+HFTYSDMSHG D+VVQKE
Sbjct: 9   NLKLVFLIVVLISCVEYTISSFVEGNGDDQPFDFSVEDATENHFTYSDMSHGFDRVVQKE 68

Query: 68  DDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCR 127
            D WSMVETKQNQFVVDGQPFYVNGFNTYWLM+FAADQSTRGKVTEVFKQA+SVGLTVCR
Sbjct: 69  SDAWSMVETKQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTEVFKQAASVGLTVCR 128

Query: 128 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIK 187
           TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSL+NNWEA+GGKAQY+K
Sbjct: 129 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLANNWEAFGGKAQYVK 188

Query: 188 WGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPR 247
           WG+AAGLNLTSDDDFF+DPTLRSYYKAHVKTVLNRVNT+TNVTYKEDPTIF WELMNEPR
Sbjct: 189 WGKAAGLNLTSDDDFFTDPTLRSYYKAHVKTVLNRVNTYTNVTYKEDPTIFAWELMNEPR 248

Query: 248 CTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 307
           CTSDPSG+ LQGWIQEMAV+VKS+DPKHL+EVGLEGFYGPSTPNRVQFNPNTYAQQVGTD
Sbjct: 249 CTSDPSGNTLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 308

Query: 308 FIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 367
           FIRNHKVLGVDFASVHIYADTWVS  ISD HLQFTKSWMEAHIEDAEKYLGMPVIFAEFG
Sbjct: 309 FIRNHKVLGVDFASVHIYADTWVSQAISDAHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 368

Query: 368 VSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSK 427
           VS+KDPGYNST+RD  L+ VYKTLLDSTKKGGSGGGSL+WQLFPEGT+YMDDGYAIVLS 
Sbjct: 369 VSTKDPGYNSTYRDKYLSSVYKTLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGYAIVLSN 428

Query: 428 SPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SPSTSNIISL STR+SIFNSICSMKCRWGCKKKNVMD+ FL+H+DHDEM
Sbjct: 429 SPSTSNIISLHSTRMSIFNSICSMKCRWGCKKKNVMDIIFLNHNDHDEM 477

[Close]

BLAST of MS010066 vs. NCBI nr
Match: XP_022938707.1 (mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >XP_022938708.1 mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata])

HSP 1 Score: 862.4 bits (2227), Expect = 1.7e-246
Identity = 417/471 (88.54%), Postives = 443/471 (94.06%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLE--GDGDNQKF-------TEDHFTYSDMSHGLDQVVQ 67
           SL L+ LIAVFI SVE TVS+ +E  GDGD+Q+F        E+HFTYSDMS+G D VVQ
Sbjct: 9   SLILIGLIAVFIFSVEHTVSSFVEGDGDGDDQQFDFSVEDVPENHFTYSDMSNGFDGVVQ 68

Query: 68  KEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTV 127
           K++D WSMVET+QNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTE+FKQA+SVGLTV
Sbjct: 69  KKNDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTELFKQAASVGLTV 128

Query: 128 CRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQY 187
           CRTWAFNDGQWRALQKSPSVYDEEVFK LDFVISEAKK+KIRLILSL+NNWEAYGGKAQY
Sbjct: 129 CRTWAFNDGQWRALQKSPSVYDEEVFKALDFVISEAKKYKIRLILSLANNWEAYGGKAQY 188

Query: 188 IKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNE 247
           +KWGRAAGLNLTSDDDFFSDPTLRSYYKAHV TVLNRVNT+TNV YKEDPTIF WELMNE
Sbjct: 189 VKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVATVLNRVNTYTNVMYKEDPTIFAWELMNE 248

Query: 248 PRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVG 307
           PRCTSDPSGDKLQGWIQEMAV+VKS+DPKHLVEVGLEGFYGPSTPNRVQ NPNTYAQQVG
Sbjct: 249 PRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLVEVGLEGFYGPSTPNRVQLNPNTYAQQVG 308

Query: 308 TDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAE 367
           TDFIRNHKVLGVDFASVHIYADTWVSP++SDTHLQFTKSWM+AHIEDAEKYLGMPVIFAE
Sbjct: 309 TDFIRNHKVLGVDFASVHIYADTWVSPSVSDTHLQFTKSWMDAHIEDAEKYLGMPVIFAE 368

Query: 368 FGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVL 427
           FGVSSKDPGYNSTFRDA LNLVYKTLL STKKGGSGGGSL+WQLFP+GT+YMDDGYAIVL
Sbjct: 369 FGVSSKDPGYNSTFRDAVLNLVYKTLLGSTKKGGSGGGSLVWQLFPDGTDYMDDGYAIVL 428

Query: 428 SKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SKSPSTSNIISL STRLSIFNSICSM+CRWGCKKKN+MDL F DHHDHDEM
Sbjct: 429 SKSPSTSNIISLHSTRLSIFNSICSMRCRWGCKKKNLMDLIFPDHHDHDEM 479

[Close]

BLAST of MS010066 vs. ExPASy Swiss-Prot
Match: Q9LZV3 (Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 SV=1)

HSP 1 Score: 663.7 bits (1711), Expect = 1.6e-189
Identity = 297/405 (73.33%), Postives = 356/405 (87.90%), Query Frame = 0


Query: 61  DDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCR 120
           ++ W MV+ K  QF ++GQPFYVNGFNTYW+M  AAD STRGKVTEVF+QAS+VG+TV R
Sbjct: 42  EEQWEMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGR 101

Query: 121 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIK 180
           TWAFNDGQWRALQKSPSVYDEEVFK LDFV+SEA+K+KIRLILSL NNW+AYGGKAQY+K
Sbjct: 102 TWAFNDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVK 161

Query: 181 WGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPR 240
           WG A+GLNLTSDDDFF++PTLR++Y++HV+TVLNRVNTFTN+TYK DPTIF WELMNEPR
Sbjct: 162 WGNASGLNLTSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPR 221

Query: 241 CTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 300
           C SDPSGDKLQ WIQEMAV+VKS+D KHLVE+GLEGFYGPS P R +FNPN YA QVGTD
Sbjct: 222 CPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTD 281

Query: 301 FIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 360
           FIRN++VLG+DFASVH+Y D+W+SP +S++ L+FT SWM+AH+EDAE YLGMPV+F EFG
Sbjct: 282 FIRNNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFG 341

Query: 361 VSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSK 420
           VS+ DPG+N++FRD  LN VYK  L+ST+KGG+G GSL+WQ+FP+G E+MDDGYA+ L++
Sbjct: 342 VSAHDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTR 401

Query: 421 SPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHD 466
           + + S IISLQS RL+IFNS+CS +CRWGCKKKN   LD L  HD
Sbjct: 402 AHTASKIISLQSKRLAIFNSLCSWRCRWGCKKKNQTALDALLSHD 446

[Close]

BLAST of MS010066 vs. ExPASy Swiss-Prot
Match: Q5W6G0 (Putative mannan endo-1,4-beta-mannosidase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN5 PE=2 SV=2)

HSP 1 Score: 530.0 bits (1364), Expect = 2.7e-149
Identity = 254/417 (60.91%), Postives = 310/417 (74.34%), Query Frame = 0


Query: 64  WSMVETKQNQFVV-DGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTW 123
           W MV T+  QFVV  G+PFYVNGFNTYWLMV A D STRGKVTEVF+QA++VGLTVCRTW
Sbjct: 75  WGMVRTRGAQFVVGGGRPFYVNGFNTYWLMVLAVDPSTRGKVTEVFRQAAAVGLTVCRTW 134

Query: 124 AFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWG 183
           AFNDG WRALQKSP VYDEEVFK LDFV+SEA+K KIRLIL L NNW+ YGGKAQY++W 
Sbjct: 135 AFNDGGWRALQKSPGVYDEEVFKALDFVVSEARKHKIRLILPLINNWDDYGGKAQYVRWA 194

Query: 184 RAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCT 243
           +AA       D FFSD T+R Y+K+HV  VL RVN +T V Y++DPTI  WELMNEPRC 
Sbjct: 195 QAAAAG-AGADAFFSDETVRGYFKSHVTAVLTRVNAYTGVAYRDDPTIMAWELMNEPRCA 254

Query: 244 SDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPST-PNRVQFNPNTYAQQVGTDF 303
           SDP+GD LQ WI EMA +VKS+DP HL+ VG EGFYGPS+ P R++ NPN      G DF
Sbjct: 255 SDPTGDTLQAWIAEMAFHVKSVDPAHLLGVGAEGFYGPSSPPARLRVNPNADVALAGADF 314

Query: 304 IRNHKVLGVDFASVHIYADTWV-SPTISDTHLQFTKSWMEAHIEDAEKYL-GMPVIFAEF 363
           +RNH+VLGVDFASVH+Y DTW+ +    +  L+F  SW+EAHI DAE  L GMPV+FAEF
Sbjct: 315 VRNHRVLGVDFASVHVYPDTWLPAGATKEAQLRFATSWVEAHIADAEGALGGMPVLFAEF 374

Query: 364 GVSSKD--PGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIV 423
           GVS++     +N+T RDA +  VY  +L ST++GG G G+LLWQ+FPEGT+YMDDGYA+V
Sbjct: 375 GVSTRGARAAFNATSRDAFIEAVYGAMLRSTRRGGGGAGALLWQVFPEGTDYMDDGYAVV 434

Query: 424 LSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKN---------VMDLDFLDHHD 466
           L ++ +T+ I++  S RL  FNS C+  CRWGC K++           D+D   HH+
Sbjct: 435 LPRAAATAGIVAAHSRRLQSFNSRCAWSCRWGCNKRDNDTAETTTAEADVDVSFHHE 490

[Close]

BLAST of MS010066 vs. ExPASy Swiss-Prot
Match: Q0IQJ7 (Mannan endo-1,4-beta-mannosidase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN8 PE=2 SV=2)

HSP 1 Score: 493.8 bits (1270), Expect = 2.1e-138
Identity = 220/364 (60.44%), Postives = 278/364 (76.37%), Query Frame = 0


Query: 66  MVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFN 125
           MVE    Q    G+PF ++GFNTYWLM FAADQ+TR +VT    +A+  GL VCRTWAF+
Sbjct: 1   MVECSGTQLWASGRPFIIHGFNTYWLMSFAADQATRPRVTAAIAEAAEAGLNVCRTWAFS 60

Query: 126 DGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAA 185
           DG +RALQ  P  YDE+VF+ LDFV+SEAK+  +RLILSL NNWE YGGKAQY++WG+ A
Sbjct: 61  DGGYRALQTVPFHYDEDVFQALDFVVSEAKRHNMRLILSLCNNWEDYGGKAQYVRWGKEA 120

Query: 186 GLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDP 245
           GL+LTS+DDFFSDPT++SYYKA V+ V+ R+NT TN TYK+DPTI  WEL+NEPRC SDP
Sbjct: 121 GLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPSDP 180

Query: 246 SGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNH 305
           SGD LQ WI+EMA YVKSIDP HL+E+G+EGFYG STP  +  NP+ Y+   GTDFIRNH
Sbjct: 181 SGDTLQAWIEEMASYVKSIDPVHLLEIGIEGFYGLSTPELLPVNPDEYSGHAGTDFIRNH 240

Query: 306 KVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKD 365
           +  G+D AS+H+Y+DTW+  +I + HLQF   WM+ HI DA   LGMP++  EFGVS KD
Sbjct: 241 QAPGIDLASIHVYSDTWLPHSIKENHLQFVDKWMQQHIHDAANLLGMPIVVGEFGVSVKD 300

Query: 366 PGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTS 425
             + + FR+  +  VY+  L S K+G  GGG LLWQLFPEG E+MDDGYA++ +KSPST 
Sbjct: 301 GKFGNEFREDFMKTVYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPSTL 360

Query: 426 NIIS 430
           ++++
Sbjct: 361 SLLA 364

[Close]

BLAST of MS010066 vs. ExPASy Swiss-Prot
Match: Q2RBB1 (Mannan endo-1,4-beta-mannosidase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=MAN7 PE=2 SV=3)

HSP 1 Score: 486.1 bits (1250), Expect = 4.4e-136
Identity = 215/366 (58.74%), Postives = 279/366 (76.23%), Query Frame = 0


Query: 64  WSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWA 123
           W+MVE +  Q    G+PF ++GFNTYWLM FAADQ+TR +VT    +A   GL VC TWA
Sbjct: 9   WTMVERRGTQLWASGRPFIIHGFNTYWLMSFAADQATRLRVTAAIAEA---GLNVCCTWA 68

Query: 124 FNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGR 183
           F+DG +RALQ +P  YDE+VF+ LDFV+SEA++  +RLILSL NNWE YGGKAQY++WG+
Sbjct: 69  FSDGGYRALQTAPFHYDEDVFRALDFVVSEARRHNMRLILSLCNNWEDYGGKAQYVRWGK 128

Query: 184 AAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTS 243
            AGL+LTS+DDFFSDPT++SYYKA V+ V+ R+NT TN TYK+DPTI  WEL+NEPRC S
Sbjct: 129 EAGLDLTSEDDFFSDPTIKSYYKAFVEAVVTRINTVTNETYKDDPTILAWELINEPRCPS 188

Query: 244 DPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIR 303
           DPSGD LQ W++EMA YVKSIDP HL+E+G+EGFYGPS P  +  NP+ Y+   G DFIR
Sbjct: 189 DPSGDTLQAWMEEMASYVKSIDPVHLLEIGIEGFYGPSIPELLPVNPDEYSGHAGIDFIR 248

Query: 304 NHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSS 363
           NH+  G+D AS+H+Y+D W+  +I + HLQF   WM+ HI+DA   LGMP++  EFGVS 
Sbjct: 249 NHQAPGIDLASIHVYSDIWLPQSIKENHLQFVDKWMQQHIDDAANLLGMPIVVGEFGVSV 308

Query: 364 KDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPS 423
           KD  + + FR+  +  +Y+  L S K+G  GGG LLWQLFPEG E+MDDGYA++ +KSPS
Sbjct: 309 KDGKFGNEFREDFMKTIYRIFLSSWKEGVIGGGCLLWQLFPEGAEHMDDGYAVIFAKSPS 368

Query: 424 TSNIIS 430
           T ++++
Sbjct: 369 TLSLLA 371

[Close]

BLAST of MS010066 vs. ExPASy Swiss-Prot
Match: Q9FZ03 (Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 SV=2)

HSP 1 Score: 424.5 bits (1090), Expect = 1.6e-117
Identity = 196/359 (54.60%), Postives = 254/359 (70.75%), Query Frame = 0


Query: 74  FVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFNDGQWRALQ 133
           F ++G PF  NGFN+YWLM  AA+ S R KV+EV ++ASS GL+VCRTWAF+DG  RALQ
Sbjct: 47  FELNGSPFLFNGFNSYWLMHVAAEPSERYKVSEVLREASSAGLSVCRTWAFSDGGDRALQ 106

Query: 134 KSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAGLNLTSDD 193
            SP VYDE VF+GLDFVISEAKK+ IRLILS  NN+  +GGKAQY++W R AG  +  DD
Sbjct: 107 ISPGVYDERVFQGLDFVISEAKKYGIRLILSFVNNYNDFGGKAQYVQWARNAGAQINGDD 166

Query: 194 DFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDPSGDKLQGW 253
           DF+++   ++YYK H+K V+ R NT T +TYK+D TI  WELMNEPR  +D SG+ L  W
Sbjct: 167 DFYTNYITKNYYKNHIKKVVTRFNTITGMTYKDDSTIMAWELMNEPRNQADYSGNTLNAW 226

Query: 254 IQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNHKVLGVDFA 313
           +QEMA +VKS+D KHL+E+G+EGFYG S P R   NP     QVGTDFI NH +  +DFA
Sbjct: 227 VQEMASFVKSLDNKHLLEIGMEGFYGDSVPERKSINP---GYQVGTDFISNHLIKEIDFA 286

Query: 314 SVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKDPGYNSTFR 373
           ++H Y D W+S    D  + F + WM +H +DA+  L  P++ AEFG SS+DPGYN   R
Sbjct: 287 TIHAYTDQWLSGQSDDAQMIFMQKWMTSHWQDAKNILKKPLVLAEFGKSSRDPGYNQNIR 346

Query: 374 DAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTSNIISLQS 433
           D  ++ +Y+ +    K GG+ GGSL+WQL  +G E  +DGY I L K+PST+ II+ QS
Sbjct: 347 DTFMSTIYRNIYSLAKDGGTMGGSLIWQLVAQGMENYEDGYCIELGKNPSTAGIITSQS 402

[Close]

BLAST of MS010066 vs. ExPASy TrEMBL
Match: A0A6J1BXA2 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111006525 PE=3 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 3.9e-276
Identity = 465/465 (100.00%), Postives = 465/465 (100.00%), Query Frame = 0


Query: 5   MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW 64
           MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW
Sbjct: 1   MGASLKLVFLIAVFISSVEITVSTSLEGDGDNQKFTEDHFTYSDMSHGLDQVVQKEDDTW 60

Query: 65  SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF 124
           SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF
Sbjct: 61  SMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAF 120

Query: 125 NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA 184
           NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA
Sbjct: 121 NDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRA 180

Query: 185 AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD 244
           AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD
Sbjct: 181 AGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSD 240

Query: 245 PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN 304
           PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN
Sbjct: 241 PSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRN 300

Query: 305 HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK 364
           HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK
Sbjct: 301 HKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSK 360

Query: 365 DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST 424
           DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST
Sbjct: 361 DPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPST 420

Query: 425 SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM
Sbjct: 421 SNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 465

[Close]

BLAST of MS010066 vs. ExPASy TrEMBL
Match: A0A6J1BZ08 (Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111006525 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.1e-252
Identity = 421/421 (100.00%), Postives = 421/421 (100.00%), Query Frame = 0


Query: 49  MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF 108
           MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF
Sbjct: 1   MSHGLDQVVQKEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVF 60

Query: 109 KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN 168
           KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN
Sbjct: 61  KQASSVGLTVCRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNN 120

Query: 169 WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP 228
           WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP
Sbjct: 121 WEAYGGKAQYIKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDP 180

Query: 229 TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF 288
           TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF
Sbjct: 181 TIFGWELMNEPRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQF 240

Query: 289 NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK 348
           NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK
Sbjct: 241 NPNTYAQQVGTDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEK 300

Query: 349 YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE 408
           YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE
Sbjct: 301 YLGMPVIFAEFGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTE 360

Query: 409 YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE 468
           YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE
Sbjct: 361 YMDDGYAIVLSKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDE 420

Query: 469 M 470
           M
Sbjct: 421 M 421

[Close]

BLAST of MS010066 vs. ExPASy TrEMBL
Match: A0A0A0KJ68 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G405340 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 9.9e-248
Identity = 413/469 (88.06%), Postives = 442/469 (94.24%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLEGDGDNQKF-------TEDHFTYSDMSHGLDQVVQKE 67
           +LKLVFLI V IS VE T+S+ +EG+GD+Q F       TE+HFTYSDMSHG D+VVQKE
Sbjct: 9   NLKLVFLIVVLISCVEYTISSFVEGNGDDQPFDFSVEDATENHFTYSDMSHGFDRVVQKE 68

Query: 68  DDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCR 127
            D WSMVETKQNQFVVDGQPFYVNGFNTYWLM+FAADQSTRGKVTEVFKQA+SVGLTVCR
Sbjct: 69  SDAWSMVETKQNQFVVDGQPFYVNGFNTYWLMIFAADQSTRGKVTEVFKQAASVGLTVCR 128

Query: 128 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIK 187
           TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSL+NNWEA+GGKAQY+K
Sbjct: 129 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLANNWEAFGGKAQYVK 188

Query: 188 WGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPR 247
           WG+AAGLNLTSDDDFF+DPTLRSYYKAHVKTVLNRVNT+TNVTYKEDPTIF WELMNEPR
Sbjct: 189 WGKAAGLNLTSDDDFFTDPTLRSYYKAHVKTVLNRVNTYTNVTYKEDPTIFAWELMNEPR 248

Query: 248 CTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 307
           CTSDPSG+ LQGWIQEMAV+VKS+DPKHL+EVGLEGFYGPSTPNRVQFNPNTYAQQVGTD
Sbjct: 249 CTSDPSGNTLQGWIQEMAVFVKSMDPKHLLEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 308

Query: 308 FIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 367
           FIRNHKVLGVDFASVHIYADTWVS  ISD HLQFTKSWMEAHIEDAEKYLGMPVIFAEFG
Sbjct: 309 FIRNHKVLGVDFASVHIYADTWVSQAISDAHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 368

Query: 368 VSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSK 427
           VS+KDPGYNST+RD  L+ VYKTLLDSTKKGGSGGGSL+WQLFPEGT+YMDDGYAIVLS 
Sbjct: 369 VSTKDPGYNSTYRDKYLSSVYKTLLDSTKKGGSGGGSLVWQLFPEGTDYMDDGYAIVLSN 428

Query: 428 SPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SPSTSNIISL STR+SIFNSICSMKCRWGCKKKNVMD+ FL+H+DHDEM
Sbjct: 429 SPSTSNIISLHSTRMSIFNSICSMKCRWGCKKKNVMDIIFLNHNDHDEM 477

[Close]

BLAST of MS010066 vs. ExPASy TrEMBL
Match: A0A6J1FEU9 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111444855 PE=3 SV=1)

HSP 1 Score: 862.4 bits (2227), Expect = 8.4e-247
Identity = 417/471 (88.54%), Postives = 443/471 (94.06%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLE--GDGDNQKF-------TEDHFTYSDMSHGLDQVVQ 67
           SL L+ LIAVFI SVE TVS+ +E  GDGD+Q+F        E+HFTYSDMS+G D VVQ
Sbjct: 9   SLILIGLIAVFIFSVEHTVSSFVEGDGDGDDQQFDFSVEDVPENHFTYSDMSNGFDGVVQ 68

Query: 68  KEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTV 127
           K++D WSMVET+QNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTE+FKQA+SVGLTV
Sbjct: 69  KKNDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTELFKQAASVGLTV 128

Query: 128 CRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQY 187
           CRTWAFNDGQWRALQKSPSVYDEEVFK LDFVISEAKK+KIRLILSL+NNWEAYGGKAQY
Sbjct: 129 CRTWAFNDGQWRALQKSPSVYDEEVFKALDFVISEAKKYKIRLILSLANNWEAYGGKAQY 188

Query: 188 IKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNE 247
           +KWGRAAGLNLTSDDDFFSDPTLRSYYKAHV TVLNRVNT+TNV YKEDPTIF WELMNE
Sbjct: 189 VKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVATVLNRVNTYTNVMYKEDPTIFAWELMNE 248

Query: 248 PRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVG 307
           PRCTSDPSGDKLQGWIQEMAV+VKS+DPKHLVEVGLEGFYGPSTPNRVQ NPNTYAQQVG
Sbjct: 249 PRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLVEVGLEGFYGPSTPNRVQLNPNTYAQQVG 308

Query: 308 TDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAE 367
           TDFIRNHKVLGVDFASVHIYADTWVSP++SDTHLQFTKSWM+AHIEDAEKYLGMPVIFAE
Sbjct: 309 TDFIRNHKVLGVDFASVHIYADTWVSPSVSDTHLQFTKSWMDAHIEDAEKYLGMPVIFAE 368

Query: 368 FGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVL 427
           FGVSSKDPGYNSTFRDA LNLVYKTLL STKKGGSGGGSL+WQLFP+GT+YMDDGYAIVL
Sbjct: 369 FGVSSKDPGYNSTFRDAVLNLVYKTLLGSTKKGGSGGGSLVWQLFPDGTDYMDDGYAIVL 428

Query: 428 SKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SKSPSTSNIISL STRLSIFNSICSM+CRWGCKKKN+MDL F DHHDHDEM
Sbjct: 429 SKSPSTSNIISLHSTRLSIFNSICSMRCRWGCKKKNLMDLIFPDHHDHDEM 479

[Close]

BLAST of MS010066 vs. ExPASy TrEMBL
Match: A0A6J1JY29 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111488922 PE=3 SV=1)

HSP 1 Score: 860.1 bits (2221), Expect = 4.2e-246
Identity = 416/471 (88.32%), Postives = 441/471 (93.63%), Query Frame = 0


Query: 8   SLKLVFLIAVFISSVEITVSTSLE--GDGDNQKF-------TEDHFTYSDMSHGLDQVVQ 67
           SL L+ LIAVFI SVE TVS+ +E  GDGD+Q+F        E+HFTYSDMS+G D VVQ
Sbjct: 9   SLILIGLIAVFIFSVEHTVSSFVEGDGDGDDQQFDFSVEDVPENHFTYSDMSNGFDGVVQ 68

Query: 68  KEDDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTV 127
           K++D WSMVET+QNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQA+SVG TV
Sbjct: 69  KKNDAWSMVETRQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQAASVGFTV 128

Query: 128 CRTWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQY 187
           CRTWAFNDGQWRALQKSPSVYDEEVFK LDFVISEAKK+KIRLILSL+NNWEAYGGKAQY
Sbjct: 129 CRTWAFNDGQWRALQKSPSVYDEEVFKALDFVISEAKKYKIRLILSLANNWEAYGGKAQY 188

Query: 188 IKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNE 247
           +KWGRAAGLNLTSDDDFFSDPTLRSYYKAHV TVLNRVNT+TNV YKEDP IF WELMNE
Sbjct: 189 VKWGRAAGLNLTSDDDFFSDPTLRSYYKAHVATVLNRVNTYTNVMYKEDPAIFAWELMNE 248

Query: 248 PRCTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVG 307
           PRCTSDPSGDKLQGWIQEMAV+VKS+DPKHLVEVGLEGFYGPSTPNRVQ NPNTYAQQVG
Sbjct: 249 PRCTSDPSGDKLQGWIQEMAVFVKSMDPKHLVEVGLEGFYGPSTPNRVQLNPNTYAQQVG 308

Query: 308 TDFIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAE 367
           TDFIRNHKVLGVDFASVHIYADTWVSP++SDTHLQFTKSWM+AHIEDAEKYLGMPVIFAE
Sbjct: 309 TDFIRNHKVLGVDFASVHIYADTWVSPSVSDTHLQFTKSWMDAHIEDAEKYLGMPVIFAE 368

Query: 368 FGVSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVL 427
           FGVSSKDPGYNSTFRDA LNLVYKTLL STKKGGSGGGSL+WQLFP+GT+YMDDGYAIVL
Sbjct: 369 FGVSSKDPGYNSTFRDAVLNLVYKTLLGSTKKGGSGGGSLVWQLFPDGTDYMDDGYAIVL 428

Query: 428 SKSPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHDHDEM 470
           SKSPSTSNIISL STRLSIFNSICSM+CRWGCKKKN+MDL F DHHDHDEM
Sbjct: 429 SKSPSTSNIISLHSTRLSIFNSICSMRCRWGCKKKNLMDLIFPDHHDHDEM 479

[Close]

BLAST of MS010066 vs. TAIR 10
Match: AT5G01930.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 663.7 bits (1711), Expect = 1.1e-190
Identity = 297/405 (73.33%), Postives = 356/405 (87.90%), Query Frame = 0


Query: 61  DDTWSMVETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCR 120
           ++ W MV+ K  QF ++GQPFYVNGFNTYW+M  AAD STRGKVTEVF+QAS+VG+TV R
Sbjct: 42  EEQWEMVQRKGMQFTLNGQPFYVNGFNTYWMMTLAADNSTRGKVTEVFQQASAVGMTVGR 101

Query: 121 TWAFNDGQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIK 180
           TWAFNDGQWRALQKSPSVYDEEVFK LDFV+SEA+K+KIRLILSL NNW+AYGGKAQY+K
Sbjct: 102 TWAFNDGQWRALQKSPSVYDEEVFKALDFVLSEARKYKIRLILSLVNNWDAYGGKAQYVK 161

Query: 181 WGRAAGLNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPR 240
           WG A+GLNLTSDDDFF++PTLR++Y++HV+TVLNRVNTFTN+TYK DPTIF WELMNEPR
Sbjct: 162 WGNASGLNLTSDDDFFTNPTLRNFYQSHVRTVLNRVNTFTNITYKNDPTIFAWELMNEPR 221

Query: 241 CTSDPSGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTD 300
           C SDPSGDKLQ WIQEMAV+VKS+D KHLVE+GLEGFYGPS P R +FNPN YA QVGTD
Sbjct: 222 CPSDPSGDKLQSWIQEMAVFVKSLDAKHLVEIGLEGFYGPSAPARTRFNPNPYAAQVGTD 281

Query: 301 FIRNHKVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFG 360
           FIRN++VLG+DFASVH+Y D+W+SP +S++ L+FT SWM+AH+EDAE YLGMPV+F EFG
Sbjct: 282 FIRNNQVLGIDFASVHVYPDSWISPAVSNSFLEFTSSWMQAHVEDAEMYLGMPVLFTEFG 341

Query: 361 VSSKDPGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSK 420
           VS+ DPG+N++FRD  LN VYK  L+ST+KGG+G GSL+WQ+FP+G E+MDDGYA+ L++
Sbjct: 342 VSAHDPGFNTSFRDMMLNTVYKMTLNSTRKGGAGAGSLVWQVFPQGAEFMDDGYAVYLTR 401

Query: 421 SPSTSNIISLQSTRLSIFNSICSMKCRWGCKKKNVMDLDFLDHHD 466
           + + S IISLQS RL+IFNS+CS +CRWGCKKKN   LD L  HD
Sbjct: 402 AHTASKIISLQSKRLAIFNSLCSWRCRWGCKKKNQTALDALLSHD 446

[Close]

BLAST of MS010066 vs. TAIR 10
Match: AT5G66460.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 422.9 bits (1086), Expect = 3.3e-118
Identity = 192/375 (51.20%), Postives = 255/375 (68.00%), Query Frame = 0


Query: 67  VETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFND 126
           V TK  QF ++G P+Y NGFN YWLM  A+D S R K++  F+ AS  GLTV RTWAF+D
Sbjct: 32  VRTKGVQFSLNGYPYYANGFNAYWLMYVASDPSQRSKISTAFQDASRHGLTVARTWAFSD 91

Query: 127 GQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAG 186
           G +RALQ SP  Y+E++F+GLDF ++EA++  I++ILS +NN+E++GG+ QY+ W R+ G
Sbjct: 92  GGYRALQYSPGSYNEDMFQGLDFALAEARRHGIKIILSFANNYESFGGRKQYVDWARSRG 151

Query: 187 LNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDPS 246
             ++S+DDFF+D  ++ +YK H+K VLNR NTFT V YK+DPTI  WELMNEPRC SDPS
Sbjct: 152 RPVSSEDDFFTDSLVKDFYKNHIKAVLNRFNTFTKVHYKDDPTIMAWELMNEPRCPSDPS 211

Query: 247 GDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNHK 306
           G  +Q WI EMA +VKS+D  HL+E GLEGFYG S+P     NP     Q GTDFI N++
Sbjct: 212 GRAIQAWITEMAAHVKSLDRNHLLEAGLEGFYGQSSPQSKTLNP---PGQFGTDFIANNR 271

Query: 307 VLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKDP 366
           + G+DF +VH Y D W   +   + + F   W++AHI+DA+  L  P+I AEFG S K P
Sbjct: 272 IPGIDFVTVHSYPDEWFPDSSEQSQMDFLNKWLDAHIQDAQNVLHKPIILAEFGKSMKKP 331

Query: 367 GYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTSN 426
           GY    RD   N VY  +  S K+GG+  G L WQL   G +   DGY I+LS+S ST N
Sbjct: 332 GYTPAQRDIVFNTVYSKIYGSAKRGGAAAGGLFWQLLVNGIDNFQDGYGIILSQSSSTVN 391

Query: 427 IISLQSTRLSIFNSI 442
           +IS QS +L++   I
Sbjct: 392 VISQQSRKLTLIRKI 403

[Close]

BLAST of MS010066 vs. TAIR 10
Match: AT3G10890.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 402.1 bits (1032), Expect = 6.0e-112
Identity = 182/372 (48.92%), Postives = 250/372 (67.20%), Query Frame = 0


Query: 67  VETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFND 126
           V  K  QF+++G+PFY NGFN YWL   A D +TR K+T VF+ A+   LT+ RTW F D
Sbjct: 32  VSRKGVQFILNGKPFYANGFNAYWLAYEATDSTTRFKITYVFQNATIHDLTIVRTWGFRD 91

Query: 127 GQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAG 186
           G +RALQ +P VYDE+ F+GLDF I+EAK+  I++I++  NN+  +GG+ QY+ W +  G
Sbjct: 92  GGYRALQIAPGVYDEKTFQGLDFAIAEAKRLGIKMIITFVNNYSDFGGRKQYVDWAKNTG 151

Query: 187 LNLTSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDPS 246
            N++SDDDF+++P ++ YYK HVKT++NRVNTFT V YK++PTI GWELMNEP+C +DPS
Sbjct: 152 QNVSSDDDFYTNPLVKQYYKNHVKTMVNRVNTFTKVEYKDEPTIMGWELMNEPQCRADPS 211

Query: 247 GDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNR-VQFNPNTYAQQVGTDFIRNH 306
           G  L  W+ EMA+YVKS+D KHL+  GLEGFYG S+P R    NP   A  +GTDFI NH
Sbjct: 212 GKTLTAWMNEMALYVKSVDSKHLLSTGLEGFYGDSSPQRKTSLNP-VAANVLGTDFIANH 271

Query: 307 KVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKD 366
           K+  +DFAS+H Y D W       + L   + W+E H+EDA+  L  P+I  EFG  +  
Sbjct: 272 KLDAIDFASIHSYPDLWFPNLDEKSRLNLLRKWLECHLEDAQNILKKPLILGEFGKPTNT 331

Query: 367 PGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTS 426
           PGY    RDA  N  + T+ +S +KGG   G+L W +  +G     D  +IVLS++ +T 
Sbjct: 332 PGYTQAQRDAVFNATFDTIYESAEKGGPAAGALFWHVISDGMNNFKDPLSIVLSENSTTV 391

Query: 427 NIISLQSTRLSI 438
           NII+ +S +L +
Sbjct: 392 NIITEESRKLGL 402

[Close]

BLAST of MS010066 vs. TAIR 10
Match: AT3G10900.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 1.1e-108
Identity = 182/366 (49.73%), Postives = 238/366 (65.03%), Query Frame = 0


Query: 73  QFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFNDGQ-WRA 132
           QF+++G+PFY NGFN YWL   A D +TR K+T VF+ A+S+GLT+ RTW F DG  +RA
Sbjct: 36  QFILNGKPFYANGFNAYWLAYEATDPTTRFKITNVFQNATSLGLTIARTWGFRDGAIYRA 95

Query: 133 LQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAGLNLTS 192
           LQ +P  YDE+ F+GLDFVI+EAK+  I+LI+ L NNW+ YGGK QY+ W R+ G  ++S
Sbjct: 96  LQTAPGSYDEQTFQGLDFVIAEAKRIGIKLIILLVNNWDDYGGKKQYVDWARSKGEVVSS 155

Query: 193 DDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDPSGDKLQ 252
           +DDF+ +P ++ +YK HVKTVLNRVNTFT V YK++P I  W+LMNEPRC  D SG  L 
Sbjct: 156 NDDFYRNPVIKDFYKNHVKTVLNRVNTFTKVAYKDEPAIMAWQLMNEPRCGVDKSGKTLM 215

Query: 253 GWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNHKVLGVD 312
            WI EMA +VKS+DP HL+  G EGFYG S+P R        A  VG DFI NH +  +D
Sbjct: 216 DWINEMAPFVKSVDPNHLLSTGHEGFYGDSSPERKNSLNPVSANTVGADFIANHNIDAID 275

Query: 313 FASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKDPGYNST 372
           FAS+H  +D W      ++ L F K W+E HIEDA+  L  PVI AEFG+ S  P Y   
Sbjct: 276 FASMHCGSDLWFQRLDQNSRLAFIKRWLEGHIEDAQNILKKPVILAEFGLGSDTPRYTLA 335

Query: 373 FRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTSNIISLQ 432
            RD      Y  +  S +KGGS  G+L W++  EG        +I+LS   ST NIIS  
Sbjct: 336 NRDGVFTTTYDIIYASAQKGGSAAGALFWEVISEGMSNFAGPSSIILSDKSSTVNIISEH 395

Query: 433 STRLSI 438
           + ++ +
Sbjct: 396 ARKMGL 401

[Close]

BLAST of MS010066 vs. TAIR 10
Match: AT4G28320.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.4e-108
Identity = 182/370 (49.19%), Postives = 249/370 (67.30%), Query Frame = 0


Query: 67  VETKQNQFVVDGQPFYVNGFNTYWLMVFAADQSTRGKVTEVFKQASSVGLTVCRTWAFND 126
           V+    QFVVD +P YVNG+N+YW M  A D+ +R  V E+ +  + +GLTVCRTWAFND
Sbjct: 42  VKRNGTQFVVDDKPLYVNGWNSYWFMDHAVDEHSRNLVGEMLEAGAKMGLTVCRTWAFND 101

Query: 127 GQWRALQKSPSVYDEEVFKGLDFVISEAKKFKIRLILSLSNNWEAYGGKAQYIKWGRAAG 186
           G + ALQ SP  +DE VF+ LD VI+EA+K  +RL+LSL NN +AYGGK QY+KW    G
Sbjct: 102 GGYNALQISPGRFDERVFQALDHVIAEARKHDVRLLLSLVNNLQAYGGKTQYVKWAWQEG 161

Query: 187 LNL-TSDDDFFSDPTLRSYYKAHVKTVLNRVNTFTNVTYKEDPTIFGWELMNEPRCTSDP 246
           + L +S+D FF DP++R+Y+K ++K +L R N+ T + Y+ DPTIF WEL+NEPRCT+D 
Sbjct: 162 VGLSSSNDSFFFDPSIRNYFKNYLKVLLTRKNSVTGIEYRNDPTIFAWELINEPRCTTDV 221

Query: 247 SGDKLQGWIQEMAVYVKSIDPKHLVEVGLEGFYGPSTPNRVQFNPNTYAQQVGTDFIRNH 306
           SG  LQ WI EM  ++KSID KHL+ VGLEGFYGP++P  +  NP  +A Q+GTDF++N 
Sbjct: 222 SGKTLQDWIDEMTGFIKSIDDKHLLTVGLEGFYGPNSPKGLTVNPEQWASQLGTDFVQNS 281

Query: 307 KVLGVDFASVHIYADTWVSPTISDTHLQFTKSWMEAHIEDAEKYLGMPVIFAEFGVSSKD 366
               +DFASVHIY D W      +  L+F   WM++HIED  K L  PV+F EFG+S+++
Sbjct: 282 NSSNIDFASVHIYPDHWFHNQTFEEKLKFVVKWMQSHIEDGLKELKKPVLFTEFGLSNQN 341

Query: 367 PGYNSTFRDAALNLVYKTLLDSTKKGGSGGGSLLWQLFPEGTEYMDDGYAIVLSKSPSTS 426
             Y  + RD    +++  +  S K+  SG G+L+WQLF EG E  +D + IV  +  S  
Sbjct: 342 KDYEPSQRDKFYRIIFDVVYKSAKRKKSGAGTLVWQLFMEGMETFNDDFGIVPHEQDSIY 401

Query: 427 NIISLQSTRL 436
            ++  QS RL
Sbjct: 402 KLMIEQSCRL 411

The following BLAST results are available for this feature:

BLAST of MS010066 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134211.1	8.0e-276	100.00	mannan endo-1,4-beta-mannosidase 6 isoform X1 [Momordica charantia]	[more]
XP_022134212.1	1.1e-251	100.00	mannan endo-1,4-beta-mannosidase 6 isoform X2 [Momordica charantia]	[more]
XP_038886206.1	1.6e-247	87.85	mannan endo-1,4-beta-mannosidase 6 isoform X1 [Benincasa hispida]	[more]
XP_004149910.1	2.1e-247	88.06	mannan endo-1,4-beta-mannosidase 6 [Cucumis sativus] >KGN47821.1 hypothetical pr...	[more]
XP_022938707.1	1.7e-246	88.54	mannan endo-1,4-beta-mannosidase 6-like [Cucurbita moschata] >XP_022938708.1 man...	[more]

BLAST of MS010066 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LZV3	1.6e-189	73.33	Mannan endo-1,4-beta-mannosidase 6 OS=Arabidopsis thaliana OX=3702 GN=MAN6 PE=2 ...	[more]
Q5W6G0	2.7e-149	60.91	Putative mannan endo-1,4-beta-mannosidase 5 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q0IQJ7	2.1e-138	60.44	Mannan endo-1,4-beta-mannosidase 8 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q2RBB1	4.4e-136	58.74	Mannan endo-1,4-beta-mannosidase 7 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9FZ03	1.6e-117	54.60	Mannan endo-1,4-beta-mannosidase 2 OS=Solanum lycopersicum OX=4081 GN=MAN2 PE=2 ...	[more]

BLAST of MS010066 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BXA2	3.9e-276	100.00	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111006525 ...	[more]
A0A6J1BZ08	5.1e-252	100.00	Mannan endo-1,4-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111006525 ...	[more]
A0A0A0KJ68	9.9e-248	88.06	Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G405340 PE=3...	[more]
A0A6J1FEU9	8.4e-247	88.54	Mannan endo-1,4-beta-mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111444855 P...	[more]
A0A6J1JY29	4.2e-246	88.32	Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111488922 PE=...	[more]

BLAST of MS010066 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01930.1	1.1e-190	73.33	Glycosyl hydrolase superfamily protein	[more]
AT5G66460.1	3.3e-118	51.20	Glycosyl hydrolase superfamily protein	[more]
AT3G10890.1	6.0e-112	48.92	Glycosyl hydrolase superfamily protein	[more]
AT3G10900.1	1.1e-108	49.73	Glycosyl hydrolase superfamily protein	[more]
AT4G28320.1	2.4e-108	49.19	Glycosyl hydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001547	Glycoside hydrolase, family 5	PFAM	PF00150	Cellulase	coord: 77..366 e-value: 2.1E-15 score: 56.9
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 64..438 e-value: 5.3E-131 score: 439.0
None	No IPR available	PANTHER	PTHR31451:SF54	MANNAN ENDO-1,4-BETA-MANNOSIDASE 6	coord: 42..465
IPR045053	Mannan endo-1,4-beta-mannosidase-like	PANTHER	PTHR31451	FAMILY NOT NAMED	coord: 42..465
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 66..418

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
MS010066.1	MS010066.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071704	organic substance metabolic process
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds