MS010040 (gene) Bitter gourd (TR) v1

Overview
NameMS010040
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
Descriptionleukotriene A-4 hydrolase homolog
Locationscaffold779: 135921 .. 139135 (+)
RNA-Seq ExpressionMS010040
SyntenyMS010040
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCCTGTGGACCCACACTCTTACACAGATTCTACCCATCCTTTCACTGATCACATTTCCCTCTCTCTCTTCTTCGATTTTTCTTCCACGATCATTCATGGTTCTGCCCTAATTTCTCTCGCGAGTTCGTACTCTGGTTCTTTGTCTCTCGATACTCGTTCTCTCACCATCCATTCGATTGTTGATCCTCAATCCCGTGCTCCGATACCGTTTTCACTCGCTCCTCCCGACAAAATCAAAGGCTCTCTTCTCTCCGTTTCGCTCTCCGGCCAGTCCTCTTTCCTCGTGATTTTCAGCACTGCTCCTACGGCCTCGGCTCTGCAATGGCTGACTCCTGCCCAGACTTACAACAAGACGCATCCGTTTGTGTACACGCAGTGCCAGGCTATTCACGCTCGTTCGATTTTTCCCTGCCAGGACACTCCGGCGGCGCGGGTGCGTTATTCTGCGCGTCTGAATATCCCTGGGGAGCTCTCGGCGGTGATGGCCGCGCGAAGTGTGGAGCGCCGCTCACCAGTTGCTGGCGAGGGTTCGATGTTGGCCGGAGGGGCTGCTTCGCTGTGGAGCGGCAAAGGGAGAGTGGTGGAAGAGTTCGTGATGGAGCAGACGATTCCGCCGTACTTGTTCGCGTTTGCCGTGGGAGAGATCGATTTCAGGGAAGTGGGACCGCGGACTAGGGTTTATGGGGAAGCTGCGCCCTCGCAGCTCGACGCTGCAGCGATTGAATTTTCAGGGACAGAGGAGATGGTGGAGCAAGCCGAGAAGTTATATGGACCGTACGTTTGGGACCGATTTGATCTTCTGGTGCTACCTCCGAGCTTTCCTTACGGTGGGATGGAAAATCCGAAGATGGTGTTCTTGACGCCGACGGTGATAAAGGGGGATGCTACGGGTGCGCATGTGGTTGCTCATGAGCTTGCTCATAGCTGGACTGGGAACCTCATCACTAACAAAAACAACGAACATTTTTGGTTGAACGAGGTACTCGCTTAAAATCTACTTTTTTTCCTTTTTGTCTTCATTCCAATTGATTGGTTGCTATTTCATTTAGATATAACTAGGGTTTCCGGGGAACTTTTTACCCAAATTAAATCAAATCACACGGGTTTGTTCTGTTCTACGCTAAATCAATGATTCTAGCGAGAAGTGAGTTGTGTTTGACTTCAGTTTCTGGTTCAAGTATCGTGCGAGCCTAATTGGGGTCCTCATTGATTTATAACAACAGTTTTATCCCTTGTACTCTATATTTTTTTGCTACTGTTAGCTTTGTCTTTTTGTGCAGGCTGTTTTGTAGTGGCCTTTTTTCTGTATTCTTTCAATGGTCCAAATTAAAGTTTGGTTTTCATTTTTAAAAAGTTGTGCAAACTCTTGCCTTGATTTCTTGCTGAGGTTTTTGTGCAAATTAATTTTCTTTTCCACTCTCTTCTAGTGTTAGTGTGTGTGTGGATGGGTTGATTTGGGAAGAAAAGAGTCAAACTTAATGAGCTTAAATGGATCTCAATCTTGTTCTTCTCTGGCAGTTCTTGATGGAATAATTGGAAGATCCTAGTTTTGGCTTGTTATGATTATGGGCAATAGAACTCTCATCATGCCATTGTGCCCAGAAGAGGCTTGTTTGTTCTTTTGAGAGCCTTTGTTTCTCTATCCTTTTTCTAATGCTATTTTTCGTCGCAAATGGCGGTAGGGTTTTACGACGTATGCTGAAAGGAGAATTGTTGAGGTTGTGCAAGGGGAGGATGCAGCTACTCTCAATACAGGAATAGGTTGGAAGGGTTTGCAAGAGGATGTTGAAAGATTTAAGGATAATTTGGAATTCACGAAGCTAAAAAACAACCAAGAAGGCGTGGATCCTGATGATGTGTATTCTCAAATACCCTACGAGAAGGGTTTTCAGTTCCTTTGGCGTATTGAACGCCAGGTATGTTATAATGTTTCTTCAAACTATATCAATCCTTTTTGTTGTTTCATTGTTCGTATTTGTTCTAATGTTCATCCTCTTAATCTTCAGGTGGGAAGGCCTAAGTTTGATGAGTTTCTTAAGAAATATATTGAAACCTTTAAGTTCCAGTCAATTGATACCCAAACCGTTCTTGATTTCATGAAAGCAACCATTCCTGGAATTGAGAAAGAGGTAGATTTGGTAATGTGGACTGAGGGCACTGGTATCCCTCCAGATGCTTATGAACCAGTTTCTAGTTTGTACACGTCGATCCTAGCGCTCGCCAATGAATTTAAGCTCGGTAAAATGCCGACCGACGAAGAAACTGCTGATTGGGGAGGGCAAGAATGGGAGCTGTACTTGCAGAATCTGCCAAAATCTGTTGAAGCCTCACAGGTACTGATTTTTATGATCAACCCCTCTTTTTTCCTTGAGAATGTTATGCTACAAAGGCTGGGGTAGGAATTTGAATATATGAGTTATTAGAAAAGGGAACTCGAACCCATGACTTGTTAGAAAAGGTAGAGATGCTTTTAACTACTCAACTCAACGACAGCGACTTGATATCACTTGCCCAAAAAACGAGTGTCTTCTTCAACTTTTATCTTTCAAATTAGGTTCTTTAGTTATGAATGAGGAGTCCGTGCATCCCCAAGGATGGTGTAGTGGCAAGGACCTTGGACAGTGTGCCTTCCATGAGATCTTTGGTTTAAACCTTTGGCTGGTCATAGTATTGTTCAGCATCAATTTTGAAAAATACCGTAAGCACACAACATAAGTCACATAGCATCAATTCTTATGGTAGGGATCCACAACGTAAACTACACAGCATCAATTCTGAAAAATTAAAAAAAAGAAGTCTATATTTTCTGGAACCAAAGATGGTTCTTGATCACAATTCTACAGTTTGATGCTTCCATGAGCAATCGTGAACTTCATATGATAGTTATGTTTTCATACCATCTGTTTGATTGTGTAGGTCCAGGCGTTAGATGCCCGTTATAAATTCTCAGAATCAAAGAATTATGATGTCAAAGCTGCATTTCTTCAAATTGCAATTGTGTCGAAGTATCGAGATAGCTATGTCGAGGTCGAGAACACTTTAACGGCAGTTGGTAGAATTAAGTACCTCCGTCCTCTGTACACTGCTCTTACACAAGGCACCGGGAGGGAAGAAGAGAAGATTTTGGCGAAGAAGATATATGCCGAAGCCCGTGAGAGTTATCACCCGATCGCTCGAGGGGTCGTTGAATCCATCTTTGCCCAGAACATG

mRNA sequence

ATGGCGCCTGTGGACCCACACTCTTACACAGATTCTACCCATCCTTTCACTGATCACATTTCCCTCTCTCTCTTCTTCGATTTTTCTTCCACGATCATTCATGGTTCTGCCCTAATTTCTCTCGCGAGTTCGTACTCTGGTTCTTTGTCTCTCGATACTCGTTCTCTCACCATCCATTCGATTGTTGATCCTCAATCCCGTGCTCCGATACCGTTTTCACTCGCTCCTCCCGACAAAATCAAAGGCTCTCTTCTCTCCGTTTCGCTCTCCGGCCAGTCCTCTTTCCTCGTGATTTTCAGCACTGCTCCTACGGCCTCGGCTCTGCAATGGCTGACTCCTGCCCAGACTTACAACAAGACGCATCCGTTTGTGTACACGCAGTGCCAGGCTATTCACGCTCGTTCGATTTTTCCCTGCCAGGACACTCCGGCGGCGCGGGTGCGTTATTCTGCGCGTCTGAATATCCCTGGGGAGCTCTCGGCGGTGATGGCCGCGCGAAGTGTGGAGCGCCGCTCACCAGTTGCTGGCGAGGGTTCGATGTTGGCCGGAGGGGCTGCTTCGCTGTGGAGCGGCAAAGGGAGAGTGGTGGAAGAGTTCGTGATGGAGCAGACGATTCCGCCGTACTTGTTCGCGTTTGCCGTGGGAGAGATCGATTTCAGGGAAGTGGGACCGCGGACTAGGGTTTATGGGGAAGCTGCGCCCTCGCAGCTCGACGCTGCAGCGATTGAATTTTCAGGGACAGAGGAGATGGTGGAGCAAGCCGAGAAGTTATATGGACCGTACGTTTGGGACCGATTTGATCTTCTGGTGCTACCTCCGAGCTTTCCTTACGGTGGGATGGAAAATCCGAAGATGGTGTTCTTGACGCCGACGGTGATAAAGGGGGATGCTACGGGTGCGCATGTGGTTGCTCATGAGCTTGCTCATAGCTGGACTGGGAACCTCATCACTAACAAAAACAACGAACATTTTTGGTTGAACGAGGGTTTTACGACGTATGCTGAAAGGAGAATTGTTGAGGTTGTGCAAGGGGAGGATGCAGCTACTCTCAATACAGGAATAGGTTGGAAGGGTTTGCAAGAGGATGTTGAAAGATTTAAGGATAATTTGGAATTCACGAAGCTAAAAAACAACCAAGAAGGCGTGGATCCTGATGATGTGTATTCTCAAATACCCTACGAGAAGGGTTTTCAGTTCCTTTGGCGTATTGAACGCCAGGTGGGAAGGCCTAAGTTTGATGAGTTTCTTAAGAAATATATTGAAACCTTTAAGTTCCAGTCAATTGATACCCAAACCGTTCTTGATTTCATGAAAGCAACCATTCCTGGAATTGAGAAAGAGGTAGATTTGGTAATGTGGACTGAGGGCACTGGTATCCCTCCAGATGCTTATGAACCAGTTTCTAGTTTGTACACGTCGATCCTAGCGCTCGCCAATGAATTTAAGCTCGGTAAAATGCCGACCGACGAAGAAACTGCTGATTGGGGAGGGCAAGAATGGGAGCTGTACTTGCAGAATCTGCCAAAATCTGTTGAAGCCTCACAGGTCCAGGCGTTAGATGCCCGTTATAAATTCTCAGAATCAAAGAATTATGATGTCAAAGCTGCATTTCTTCAAATTGCAATTGTGTCGAAGTATCGAGATAGCTATGTCGAGGTCGAGAACACTTTAACGGCAGTTGGTAGAATTAAGTACCTCCGTCCTCTGTACACTGCTCTTACACAAGGCACCGGGAGGGAAGAAGAGAAGATTTTGGCGAAGAAGATATATGCCGAAGCCCGTGAGAGTTATCACCCGATCGCTCGAGGGGTCGTTGAATCCATCTTTGCCCAGAACATG

Coding sequence (CDS)

ATGGCGCCTGTGGACCCACACTCTTACACAGATTCTACCCATCCTTTCACTGATCACATTTCCCTCTCTCTCTTCTTCGATTTTTCTTCCACGATCATTCATGGTTCTGCCCTAATTTCTCTCGCGAGTTCGTACTCTGGTTCTTTGTCTCTCGATACTCGTTCTCTCACCATCCATTCGATTGTTGATCCTCAATCCCGTGCTCCGATACCGTTTTCACTCGCTCCTCCCGACAAAATCAAAGGCTCTCTTCTCTCCGTTTCGCTCTCCGGCCAGTCCTCTTTCCTCGTGATTTTCAGCACTGCTCCTACGGCCTCGGCTCTGCAATGGCTGACTCCTGCCCAGACTTACAACAAGACGCATCCGTTTGTGTACACGCAGTGCCAGGCTATTCACGCTCGTTCGATTTTTCCCTGCCAGGACACTCCGGCGGCGCGGGTGCGTTATTCTGCGCGTCTGAATATCCCTGGGGAGCTCTCGGCGGTGATGGCCGCGCGAAGTGTGGAGCGCCGCTCACCAGTTGCTGGCGAGGGTTCGATGTTGGCCGGAGGGGCTGCTTCGCTGTGGAGCGGCAAAGGGAGAGTGGTGGAAGAGTTCGTGATGGAGCAGACGATTCCGCCGTACTTGTTCGCGTTTGCCGTGGGAGAGATCGATTTCAGGGAAGTGGGACCGCGGACTAGGGTTTATGGGGAAGCTGCGCCCTCGCAGCTCGACGCTGCAGCGATTGAATTTTCAGGGACAGAGGAGATGGTGGAGCAAGCCGAGAAGTTATATGGACCGTACGTTTGGGACCGATTTGATCTTCTGGTGCTACCTCCGAGCTTTCCTTACGGTGGGATGGAAAATCCGAAGATGGTGTTCTTGACGCCGACGGTGATAAAGGGGGATGCTACGGGTGCGCATGTGGTTGCTCATGAGCTTGCTCATAGCTGGACTGGGAACCTCATCACTAACAAAAACAACGAACATTTTTGGTTGAACGAGGGTTTTACGACGTATGCTGAAAGGAGAATTGTTGAGGTTGTGCAAGGGGAGGATGCAGCTACTCTCAATACAGGAATAGGTTGGAAGGGTTTGCAAGAGGATGTTGAAAGATTTAAGGATAATTTGGAATTCACGAAGCTAAAAAACAACCAAGAAGGCGTGGATCCTGATGATGTGTATTCTCAAATACCCTACGAGAAGGGTTTTCAGTTCCTTTGGCGTATTGAACGCCAGGTGGGAAGGCCTAAGTTTGATGAGTTTCTTAAGAAATATATTGAAACCTTTAAGTTCCAGTCAATTGATACCCAAACCGTTCTTGATTTCATGAAAGCAACCATTCCTGGAATTGAGAAAGAGGTAGATTTGGTAATGTGGACTGAGGGCACTGGTATCCCTCCAGATGCTTATGAACCAGTTTCTAGTTTGTACACGTCGATCCTAGCGCTCGCCAATGAATTTAAGCTCGGTAAAATGCCGACCGACGAAGAAACTGCTGATTGGGGAGGGCAAGAATGGGAGCTGTACTTGCAGAATCTGCCAAAATCTGTTGAAGCCTCACAGGTCCAGGCGTTAGATGCCCGTTATAAATTCTCAGAATCAAAGAATTATGATGTCAAAGCTGCATTTCTTCAAATTGCAATTGTGTCGAAGTATCGAGATAGCTATGTCGAGGTCGAGAACACTTTAACGGCAGTTGGTAGAATTAAGTACCTCCGTCCTCTGTACACTGCTCTTACACAAGGCACCGGGAGGGAAGAAGAGAAGATTTTGGCGAAGAAGATATATGCCGAAGCCCGTGAGAGTTATCACCCGATCGCTCGAGGGGTCGTTGAATCCATCTTTGCCCAGAACATG

Protein sequence

MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHSIVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKTHPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSMLAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAAAIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYIETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANEFKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPIARGVVESIFAQNM
Homology
BLAST of MS010040 vs. NCBI nr
Match: XP_022133728.1 (leukotriene A-4 hydrolase homolog [Momordica charantia])

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 610/613 (99.51%), Postives = 610/613 (99.51%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS
Sbjct: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT
Sbjct: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA
Sbjct: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           AIEFSGTEEMVE AEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA
Sbjct: 241 AIEFSGTEEMVEHAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE
Sbjct: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALD RYKFSESKNYDVKAAFLQI
Sbjct: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDGRYKFSESKNYDVKAAFLQI 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQG GREEEKILAKKIYAEARESYHPI
Sbjct: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGPGREEEKILAKKIYAEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           ARGVVESIFAQNM
Sbjct: 601 ARGVVESIFAQNM 613

BLAST of MS010040 vs. NCBI nr
Match: XP_022993079.1 (leukotriene A-4 hydrolase homolog [Cucurbita maxima])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.5e-303
Identity = 513/613 (83.69%), Postives = 564/613 (92.01%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDS+HP T HISLSLFFDF ST IH SALISL++S+SG LSLD RSLTIHS
Sbjct: 1   MAPVDPHSYTDSSHPLTVHISLSLFFDFPSTTIHASALISLSTSHSGPLSLDARSLTIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++DPQS APIPFS++PPD IKGSLLSVSLSGQSSFLVI+STAP+ SALQW+TP QT+NKT
Sbjct: 61  VLDPQSHAPIPFSISPPDPIKGSLLSVSLSGQSSFLVIYSTAPSCSALQWMTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARV YSARLNIP +LSAVMAARSVERR P  GEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVCYSARLNIPRQLSAVMAARSVERRPPDVGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LW G+GRVVEEFVMEQ IPPYLFAFAVGEI F+EVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDFLWGGEGRVVEEFVMEQPIPPYLFAFAVGEIAFKEVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TGA
Sbjct: 241 AREFAGTEEMMRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLN GIGW G +
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNIGIGWNGWK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           +DVERFKDNLEFTKLK NQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI
Sbjct: 361 DDVERFKDNLEFTKLKTNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T LDF+K  +PGIEKE+DL +WTEGTGIPPDAYEPVS+LYT IL+LAN+
Sbjct: 421 ETFKFKSIDTETFLDFLKENLPGIEKEIDLELWTEGTGIPPDAYEPVSNLYTKILSLAND 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP ++ETADWGGQEWELYL+NLPKSVEASQ+QALDARY+ SESK+Y++K AFLQ+
Sbjct: 481 FKLGKMPREDETADWGGQEWELYLENLPKSVEASQIQALDARYRLSESKDYEIKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD YVEVE TL  VGR+KYLRPLY ALTQG+G EE+KILAK+IY+EARESYHPI
Sbjct: 541 AISSKFRDCYVEVEKTLKEVGRMKYLRPLYNALTQGSGMEEDKILAKRIYSEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           A+ VVE+IF++NM
Sbjct: 601 AQRVVEAIFSKNM 613

BLAST of MS010040 vs. NCBI nr
Match: KAG7033020.1 (Leukotriene A-4 hydrolase-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.5e-303
Identity = 511/613 (83.36%), Postives = 566/613 (92.33%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHS+TDS+HP T HISLSL+FDF ST IHG+AL+SL+SS+SG LSLDTRSLTIH+
Sbjct: 1   MAPVDPHSFTDSSHPLTVHISLSLYFDFPSTTIHGAALVSLSSSHSGPLSLDTRSLTIHA 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++ P+S APIPFSL+PPD IKGSLLSVSLSGQSSFLVI++T+P++SALQWLTP QT+NKT
Sbjct: 61  VLHPESHAPIPFSLSPPDSIKGSLLSVSLSGQSSFLVIYTTSPSSSALQWLTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIP ELSAVMAARSV+RR PVAGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPQELSAVMAARSVKRRPPVAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           L GG   LWSG+GRVVEEFVMEQ IPPYLFAFAVGEI FREVGPRTRVY E+ PS LDAA
Sbjct: 181 LGGGVDLLWSGEGRVVEEFVMEQPIPPYLFAFAVGEIAFREVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TG 
Sbjct: 241 AREFAGTEEMIRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGV 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFT YAERRIVEVVQGEDAATLN GIGW  L+
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTRYAERRIVEVVQGEDAATLNIGIGWNALK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLK+NQEGVDPDDVYSQIPYEKGFQF+WRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKSNQEGVDPDDVYSQIPYEKGFQFVWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T +DF+KA IPGIEKE+DL MWTEGTGIPPDAYEPVS+LYT IL+LANE
Sbjct: 421 ETFKFKSIDTETFIDFLKANIPGIEKEIDLEMWTEGTGIPPDAYEPVSNLYTKILSLANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP D+ETA WGG+EWELYL+NLPK V+ASQ+QALDARY+FS+SK+Y+VK AFLQ+
Sbjct: 481 FKLGKMPRDDETAAWGGREWELYLENLPKPVDASQIQALDARYRFSKSKDYEVKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD Y+EVE TL  VGR+KYLRPLY ALTQG G EEEKILAK+IY+EARE YHPI
Sbjct: 541 AISSKFRDCYIEVEKTLKEVGRMKYLRPLYNALTQGPGMEEEKILAKRIYSEARECYHPI 600

Query: 601 ARGVVESIFAQNM 614
           AR VVE++F++NM
Sbjct: 601 ARRVVETVFSKNM 613

BLAST of MS010040 vs. NCBI nr
Match: XP_022952720.1 (leukotriene A-4 hydrolase homolog [Cucurbita moschata])

HSP 1 Score: 1050.8 bits (2716), Expect = 4.5e-303
Identity = 511/613 (83.36%), Postives = 566/613 (92.33%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHS+TDS+HP T HISLSL+FDF ST IHG+AL+SL+SS+SG LSLDTRSLTIH+
Sbjct: 1   MAPVDPHSFTDSSHPLTVHISLSLYFDFPSTTIHGAALVSLSSSHSGPLSLDTRSLTIHA 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++ P+S APIPFSL+PPD IKGSLLSVSLSGQSSFLVI++T+P++SALQWLTP QT+NKT
Sbjct: 61  VLHPESHAPIPFSLSPPDSIKGSLLSVSLSGQSSFLVIYTTSPSSSALQWLTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIP ELSAVMAARSV+RR PVAGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPQELSAVMAARSVKRRPPVAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LWSG+GRVVEEFVMEQ IPPYLFAFAVGEI FREVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDLLWSGEGRVVEEFVMEQPIPPYLFAFAVGEIAFREVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TG 
Sbjct: 241 AREFAGTEEMIRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGV 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFT YAERRIVEVVQGEDAATLN GIGW  L+
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTRYAERRIVEVVQGEDAATLNIGIGWNALK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLK+NQEGVDPDDVYSQIPYEKGFQF+WRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKSNQEGVDPDDVYSQIPYEKGFQFVWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T +DF+KA IPGIEKE+DL MWTEGTGIPPDAYEPVS+LYT IL+LANE
Sbjct: 421 ETFKFKSIDTETFIDFLKANIPGIEKEIDLEMWTEGTGIPPDAYEPVSNLYTKILSLANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP D+ETA WGG+EWELYL+NLPK V+ASQ+QALD RY+FS+SK+Y+VK AFLQ+
Sbjct: 481 FKLGKMPRDDETAAWGGREWELYLENLPKPVDASQIQALDTRYRFSKSKDYEVKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD Y+EVE TL  VGR+KYLRPLY ALTQG G EEEKILAK+IY+EARE YHPI
Sbjct: 541 AISSKFRDCYIEVEKTLKEVGRMKYLRPLYNALTQGPGMEEEKILAKRIYSEARECYHPI 600

Query: 601 ARGVVESIFAQNM 614
           AR VVE++F++NM
Sbjct: 601 ARRVVETVFSKNM 613

BLAST of MS010040 vs. NCBI nr
Match: KAG6578737.1 (Leucine aminopeptidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1046.6 bits (2705), Expect = 8.5e-302
Identity = 510/613 (83.20%), Postives = 566/613 (92.33%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDS+HP T HISLSLFFDF ST IH SALISL++S+SG LSLD RSL IHS
Sbjct: 1   MAPVDPHSYTDSSHPLTVHISLSLFFDFPSTTIHASALISLSTSHSGPLSLDARSLIIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++DPQS+APIPFS++PPD IKGSL+SVSLSGQSSFLVI+STAP+ SALQW+TP QT+NKT
Sbjct: 61  VLDPQSQAPIPFSISPPDSIKGSLVSVSLSGQSSFLVIYSTAPSCSALQWMTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARV +SARLNIP +LSAVMAARSVERR P AGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVCFSARLNIPRQLSAVMAARSVERRPPGAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LW G+GRVVEEFVMEQ IPPYLFAFAVGEI F+EVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDLLWGGEGRVVEEFVMEQPIPPYLFAFAVGEIAFKEVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TGA
Sbjct: 241 AREFAGTEEMMRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQG+DAATLN GIGW G +
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGKDAATLNIGIGWNGWK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           +DVERFKDNLEFTKLK NQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI
Sbjct: 361 DDVERFKDNLEFTKLKTNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T LDF+KA +PGIEKE++L +WTEGTGIPPDAYEPVS+LYT IL+LAN+
Sbjct: 421 ETFKFKSIDTETFLDFLKANLPGIEKEINLELWTEGTGIPPDAYEPVSNLYTKILSLAND 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP ++ETADWGGQEWELYL+NLPKSVEASQ+QALDARY+ SESK+Y++K AFLQ+
Sbjct: 481 FKLGKMPREDETADWGGQEWELYLENLPKSVEASQIQALDARYRLSESKDYEIKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD YVEVE TL  VGR+KYLRPLY ALTQG+G EE+KILAK+IY+EARESYHPI
Sbjct: 541 AISSKFRDCYVEVEKTLKEVGRMKYLRPLYNALTQGSGMEEDKILAKRIYSEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           A+ VVE+IF++NM
Sbjct: 601 AQRVVEAIFSKNM 613

BLAST of MS010040 vs. ExPASy Swiss-Prot
Match: Q9FY49 (Leucine aminopeptidase OS=Arabidopsis thaliana OX=3702 GN=LKHA4 PE=2 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 2.4e-254
Identity = 429/615 (69.76%), Postives = 524/615 (85.20%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAP+DPHS+TDS+HP T H++LSL+ DF+++IIHGSAL++L+S++SG LSLDTR ++I  
Sbjct: 1   MAPIDPHSFTDSSHPLTTHVALSLYLDFNTSIIHGSALLTLSSAFSGELSLDTRCISIAM 60

Query: 61  IVDPQSRAPIPFSLA-PPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNK 120
           ++DP +  PIP+S++  PD+I+G+ + V LSGQSS L+++ST+P+ASALQWL+P QT++K
Sbjct: 61  VLDPLTLEPIPYSVSTTPDRIRGTEVVVVLSGQSSLLIVYSTSPSASALQWLSPLQTFSK 120

Query: 121 THPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGS 180
            HP+VYTQCQAIHARSIFPCQDTPAAR+RY   +NIP  LSAVM+AR V RR  V  E  
Sbjct: 121 LHPYVYTQCQAIHARSIFPCQDTPAARIRYDVVMNIPNSLSAVMSARHVRRRLAVPEEAK 180

Query: 181 MLAGGA--ASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQ- 240
            L  G+  +SLW G+ RVVEEF MEQ IPPYLFAFAVGE+ FREVGPRTRVY E+A  + 
Sbjct: 181 HLEAGSLGSSLWCGEDRVVEEFAMEQPIPPYLFAFAVGELGFREVGPRTRVYTESAAIEV 240

Query: 241 LDAAAIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD 300
           LDAAA+EF+GTE+M++Q EKL+G Y W+RFDLLVLPPSFPYGGMENP+MVFLTPTVIKGD
Sbjct: 241 LDAAALEFAGTEDMIKQGEKLFGDYEWERFDLLVLPPSFPYGGMENPRMVFLTPTVIKGD 300

Query: 301 ATGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGW 360
           ATGA VVAHELAHSWTGNLITN NNEHFWLNEGFTTYAERRIVEVVQG D ATLN GIGW
Sbjct: 301 ATGAQVVAHELAHSWTGNLITNINNEHFWLNEGFTTYAERRIVEVVQGADIATLNIGIGW 360

Query: 361 KGLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFL 420
           +GL +++ERFKDNLE TKL N QEGVDPDDVYSQ+PYEKGFQF+ RIERQ+GR  FDEFL
Sbjct: 361 RGLTDEMERFKDNLECTKLWNKQEGVDPDDVYSQVPYEKGFQFVLRIERQIGRTAFDEFL 420

Query: 421 KKYIETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILA 480
           KKYI TFKF+SIDT T L+F+KA IPGIEKE++L +WTEG GIP DAYEPVS++YT I++
Sbjct: 421 KKYIATFKFKSIDTNTFLEFLKANIPGIEKEINLQLWTEGVGIPEDAYEPVSTIYTKIIS 480

Query: 481 LANEFKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAA 540
           LA EFK GKMP++++ A+W GQEWELYL+NLPKS E SQV ALD RY+ +ESK+Y+VK +
Sbjct: 481 LAKEFKEGKMPSEDDVAEWNGQEWELYLENLPKSCEPSQVMALDKRYRLAESKDYEVKVS 540

Query: 541 FLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARES 600
           FLQ+A+ SK R+ + EV+ TL  VGR+KYLRPL+TAL Q +G  EEK LAK+++AEARE+
Sbjct: 541 FLQLAVTSKCREYHGEVKKTLKEVGRMKYLRPLFTALAQ-SGGTEEKQLAKQVFAEARET 600

Query: 601 YHPIARGVVESIFAQ 612
           YHPIA+GVVESI ++
Sbjct: 601 YHPIAQGVVESILSK 614

BLAST of MS010040 vs. ExPASy Swiss-Prot
Match: Q84TA3 (Leucine aminopeptidase OS=Oryza sativa subsp. japonica OX=39947 GN=LKHA4 PE=2 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 1.1e-224
Identity = 390/615 (63.41%), Postives = 476/615 (77.40%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           M PVDPHSYTD  HP T   +L+ + DF+++ IH SAL++L++ +SG L LDTR+L +HS
Sbjct: 1   MPPVDPHSYTDGDHPVTAKAALAFYLDFAASTIHASALLTLSAPHSGDLLLDTRALAVHS 60

Query: 61  IVDPQS-RAPIPFSLA-PPDKIKGSLLSVSL-SGQSSFLVIFSTAPTASALQWLTPAQTY 120
                   +PIPFSLA   D + GS L+++L    +SFL+ FST+P+ASALQWL+P QT 
Sbjct: 61  ASTASGPPSPIPFSLADAADPVLGSALTLTLPPDTTSFLLTFSTSPSASALQWLSPPQTA 120

Query: 121 NKTHPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGE 180
           +   PFV++QCQ+IHARS+FPC DTPAAR+ +   LN+P +LSAV AAR V RR P+  +
Sbjct: 121 SSL-PFVFSQCQSIHARSVFPCHDTPAARITFDLLLNVPTQLSAVAAARHVSRRDPLPSD 180

Query: 181 GSMLAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQL 240
                  A  LW   GR+VEEF MEQ++PPYLFAFA G I FR++GPRTRVY E     L
Sbjct: 181 HRGACDDA--LWCAPGRIVEEFQMEQSVPPYLFAFAAGGIGFRDLGPRTRVYAEGGDKVL 240

Query: 241 DAAAIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDA 300
           D AA EF+G EEMV+  E L+GPY W+RFDLLVLPPSFPYGGMENP+MVFLTPTVIKGDA
Sbjct: 241 DEAAREFAGVEEMVKVGESLFGPYEWERFDLLVLPPSFPYGGMENPRMVFLTPTVIKGDA 300

Query: 301 TGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWK 360
            GA VVAHELAHSWTGNLITNK NE FWLNEGFTTYAERRIVEVVQGE+ A LN GIGW+
Sbjct: 301 AGAQVVAHELAHSWTGNLITNKTNEDFWLNEGFTTYAERRIVEVVQGEERAALNMGIGWR 360

Query: 361 GLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLK 420
           GL   +ERFKDN+E+TKLK    G+DPDDVYS++PYEKGFQFLWRIERQ+GRP FDEFLK
Sbjct: 361 GLNRMMERFKDNMEYTKLKPKMAGIDPDDVYSEVPYEKGFQFLWRIERQIGRPAFDEFLK 420

Query: 421 KYIETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILAL 480
            YI TFKF+SIDT+T L+F+K  +PGIE ++DL +W EGTGIPPDA EP S++Y  I +L
Sbjct: 421 NYISTFKFKSIDTETFLEFLKTNVPGIENQIDLQLWIEGTGIPPDAMEPESAIYKKICSL 480

Query: 481 ANEFKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAF 540
           A EFK GK+P+++E ADW GQEWELYL+NLP  VEASQV ALD RYK SES +Y+VK AF
Sbjct: 481 AAEFKSGKLPSEDEVADWSGQEWELYLENLPTDVEASQVTALDERYKLSESCDYEVKVAF 540

Query: 541 LQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESY 600
           LQ+AI +  R  + EVE  L  VGR+KYLRPLY++L + +G  EEK+LA +I++EA E Y
Sbjct: 541 LQLAIPTGCRCYFNEVEKCLKQVGRMKYLRPLYSSLARCSG--EEKMLAHRIFSEAHEFY 600

Query: 601 HPIARGVVESIFAQN 613
           HPIAR V ESI +++
Sbjct: 601 HPIARSVAESILSKH 610

BLAST of MS010040 vs. ExPASy Swiss-Prot
Match: P09960 (Leukotriene A-4 hydrolase OS=Homo sapiens OX=9606 GN=LTA4H PE=1 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 3.0e-100
Identity = 226/609 (37.11%), Postives = 335/609 (55.01%), Query Frame = 0

Query: 17  TDHISLSLFFDFSSTIIHGSALISLASSYSG--SLSLDTRSLTIHSIVDPQSRAPIPFSL 76
           T H+ L    DF+   + G+A +++ S      SL LDT+ LTI  +V   +   + ++L
Sbjct: 19  TKHLHLRCSVDFTRRTLTGTAALTVQSQEDNLRSLVLDTKDLTIEKVV--INGQEVKYAL 78

Query: 77  APPDKIKGSLLSVSLS---GQSSFLVI---FSTAPTASALQWLTPAQTYNKTHPFVYTQC 136
                 KGS + +SL     ++  +VI   F T+P +SALQWLTP QT  K HP++++QC
Sbjct: 79  GERQSYKGSPMEISLPIALSKNQEIVIEISFETSPKSSALQWLTPEQTSGKEHPYLFSQC 138

Query: 137 QAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAA-RSVERRSPVAGEGSMLAGGAAS 196
           QAIH R+I PCQDTP+ ++ Y+A +++P EL A+M+A R  E   P              
Sbjct: 139 QAIHCRAILPCQDTPSVKLTYTAEVSVPKELVALMSAIRDGETPDP-------------- 198

Query: 197 LWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAAAIEFSGT 256
                 R + +F+ +  IP YL A  VG ++ R++GPRT V+ E    Q++ +A EFS T
Sbjct: 199 --EDPSRKIYKFIQKVPIPCYLIALVVGALESRQIGPRTLVWSE--KEQVEKSAYEFSET 258

Query: 257 EEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGAHVVAHEL 316
           E M++ AE L GPYVW ++DLLVLPPSFPYGGMENP + F+TPT++ GD + ++V+AHE+
Sbjct: 259 ESMLKIAEDLGGPYVWGQYDLLVLPPSFPYGGMENPCLTFVTPTLLAGDKSLSNVIAHEI 318

Query: 317 AHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQEDVERFK 376
           +HSWTGNL+TNK  +HFWLNEG T Y ER I   + GE     N   GW  LQ  V+ F 
Sbjct: 319 SHSWTGNLVTNKTWDHFWLNEGHTVYLERHICGRLFGEKFRHFNALGGWGELQNSVKTFG 378

Query: 377 DNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPK-FDEFLKKYIETFKFQ 436
           +   FTKL  +   +DPD  YS +PYEKGF  L+ +E+ +G P+ F  FLK Y+E F ++
Sbjct: 379 ETHPFTKLVVDLTDIDPDVAYSSVPYEKGFALLFYLEQLLGGPEIFLGFLKAYVEKFSYK 438

Query: 437 SIDTQTVLDFMKATIP---GIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILAL------ 496
           SI T    DF+ +       +  +VD   W    G+PP       +L  + +AL      
Sbjct: 439 SITTDDWKDFLYSYFKDKVDVLNQVDWNAWLYSPGLPPIKPNYDMTLTNACIALSQRWIT 498

Query: 497 ANEFKLGKMPTDEETADWGGQEWELYLQNLPKS-VEASQVQALDARYKFSESKNYDVKAA 556
           A E  L      +       Q  E   Q L ++ +    ++ +   Y F+   N +++  
Sbjct: 499 AKEDDLNSFNATDLKDLSSHQLNEFLAQTLQRAPLPLGHIKRMQEVYNFNAINNSEIRFR 558

Query: 557 FLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARES 606
           +L++ I SK+ D+        T  GR+K+ RPL+  L       ++   A + Y E + S
Sbjct: 559 WLRLCIQSKWEDAIPLALKMATEQGRMKFTRPLFKDLAAFDKSHDQ---AVRTYQEHKAS 604

BLAST of MS010040 vs. ExPASy Swiss-Prot
Match: Q7S785 (Leucine aminopeptidase 2 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ara-1 PE=3 SV=2)

HSP 1 Score: 367.1 bits (941), Expect = 3.9e-100
Identity = 242/630 (38.41%), Postives = 356/630 (56.51%), Query Frame = 0

Query: 1   MAPV-DPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLAS---SYSGSLSLDTRSL 60
           MAPV DP++ ++     T H + +L  DF++  + GS ++ L S     S  + LD+  +
Sbjct: 54  MAPVRDPNTLSNYDAWRTRHTTANLKIDFTAKCLRGSVILELESQTDKASKEIVLDSSYV 113

Query: 61  TIHSIVDPQSRAPIPFSLAPPDKIKGSLLSVSL-SGQSSFLVI-----FSTAPTASALQW 120
           T++SI    + AP  +      +  GS + +++  G +   V+      +T    +ALQW
Sbjct: 114 TVNSI--KLNSAPSLWETKARTEPNGSPVHIAVPEGAAKGEVVKVEIELATTDKCTALQW 173

Query: 121 LTPAQTYNKTHPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVER 180
           LTPAQT +K  PF+++QCQAIHARS+FPCQDTP  +  Y   +  P     V+ A  V  
Sbjct: 174 LTPAQTSDKAAPFMFSQCQAIHARSLFPCQDTPDVKSTYDFNITSP----YVVVASGV-- 233

Query: 181 RSPVAGEGSMLAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYG 240
             PV  E   L  G   L+        +F  +  IP YLFA + GEI    VG R+ V  
Sbjct: 234 --PVPDETKDL--GEEKLY--------KFQQKVPIPSYLFALSSGEIASAPVGKRSCVC- 293

Query: 241 EAAPSQLDAAAIEFSG-TEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLT 300
              P++L A+  E  G  ++ +E AEK+  PY W  +++LVLPPSFPYGGMENP   F T
Sbjct: 294 -TGPNELKASQWELEGDMDKFLEAAEKIVFPYRWGEYNVLVLPPSFPYGGMENPIFTFAT 353

Query: 301 PTVIKGDATGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAAT 360
           PT+I GD     V+AHELAHSW+GNL+T+ + EHFWLNEG+T Y ERRI+  + G DA  
Sbjct: 354 PTIISGDKQNIDVIAHELAHSWSGNLVTSCSWEHFWLNEGWTMYLERRILASIHGGDAHF 413

Query: 361 LNTGI-GWKGLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVG 420
             + I GWK L+E ++ + ++ EFTKL  + +G+DPDD +S +PYEKGF F+W ++R VG
Sbjct: 414 DFSAIRGWKALEEAIKEYGEDHEFTKLCISHKGIDPDDAFSTVPYEKGFHFVWSLDRLVG 473

Query: 421 RPKFDEFLKKYIETFKFQSIDT----QTVLDFMKA-TIPGIEKEVDLVMWT---EGTGIP 480
           R  FD+F+  Y   +  +S+D+     T L+F  A     ++ ++  + W      TG+P
Sbjct: 474 RENFDKFIPYYFGKWSNKSLDSYEFKDTFLEFFSAPEYSDLKDKIASIDWEGRFHSTGLP 533

Query: 481 PDAYEPVSSLYTSILALANEFKLGKM-PTDEETADWGGQEWELYL---QNLPKSVEASQV 540
           P   E  +SL      LA ++K     P+  + A W G +  ++L   Q+  + +   Q 
Sbjct: 534 PKP-EFDTSLADVCYELAEKWKSKDFTPSPSDVASWTGNQVLVFLNAVQDFEEPLTVEQS 593

Query: 541 QALDARYKFSESKNYDVKAAFLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQG 600
           QAL   Y  SESKN ++KAA+  IA+ SK   +Y  V + L  VGR+K++RPL+  L   
Sbjct: 594 QALGKAYGLSESKNAELKAAYYHIAMRSKDASAYQGVADLLGEVGRMKFVRPLFRGL--- 653

Query: 601 TGREEEKILAKKIYAEARESYHPIARGVVE 607
              + ++ LA K + + RE YHPI R +VE
Sbjct: 654 --NKVDRELALKTFEKNREFYHPICRQMVE 655

BLAST of MS010040 vs. ExPASy Swiss-Prot
Match: A7EJL9 (Leucine aminopeptidase 2 OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 / Ss-1) OX=665079 GN=SS1G_05513 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 6.7e-100
Identity = 231/629 (36.72%), Postives = 342/629 (54.37%), Query Frame = 0

Query: 3   PVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLAS---SYSGSLSLDTRSLTIH 62
           P DP++ ++  +  T H    L  DF    +HG+  + L S     S  + LDT  + + 
Sbjct: 2   PRDPNTLSNYNNWRTKHTIADLAIDFKKQRVHGTVTLQLESITDKESEEIILDTSFVDVQ 61

Query: 63  SIVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQS------SFLVIFSTAPTASALQWLTP 122
            I    S+    + L   ++  GS LSV + G +      +  +  ST    +ALQWLTP
Sbjct: 62  KIAVDGSKTE-EWVLKERNEPYGSPLSVKIPGGAAKGTIIALDITISTTDKCTALQWLTP 121

Query: 123 AQTYNKTHPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSP 182
           AQT NK  P++++QCQAIH RSIFPCQDTP  +  Y  R+                 RSP
Sbjct: 122 AQTSNKKFPYMFSQCQAIHNRSIFPCQDTPDVKSTYDFRI-----------------RSP 181

Query: 183 VAGEGSMLAGGAASLWSGK----GRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVY 242
           +    S L  GAAS   G+    G ++  F  E  +P YLFA + G+I    +G R+ V 
Sbjct: 182 LPVLASGLPRGAASFVHGENGESGTLLYSFYQEIPMPSYLFALSSGDIATASIGSRSLV- 241

Query: 243 GEAAPSQLDAAAIEFS-GTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFL 302
               P +L  A  E    TE+ +E  EK+  PY W ++++LVLPPSFPYGGMENP   F 
Sbjct: 242 -STGPEELLGAKWELERDTEKFIETIEKIVYPYEWTQYNVLVLPPSFPYGGMENPVFTFA 301

Query: 303 TPTVIKGDATGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAA 362
           TPT+I GD     V+AHELAHSW+GNL++N + EHFWLNEG+T Y ERRI+  V GE   
Sbjct: 302 TPTIISGDRENVDVIAHELAHSWSGNLVSNASWEHFWLNEGWTVYLERRIIAAVHGEAYR 361

Query: 363 TLNTGIGWKGLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVG 422
             ++ IGWK L++ V+ F ++ EFTKL  + +G DPDD +S +PYEKGF FL+ +ER VG
Sbjct: 362 DFSSIIGWKALEDSVKLFGEDHEFTKLVVDLKGKDPDDAFSSVPYEKGFHFLYYLERLVG 421

Query: 423 RPKFDEFLKKYIETFKFQSIDT----QTVLDFM---KATIPGIEKEVDLVMWTEGTGIPP 482
           +P +D+F+  Y  T+K +S+D+     T+LDF    +A    +E  VD   W    G+PP
Sbjct: 422 KPTWDKFIPHYFTTWKKKSLDSYEFKATLLDFFASDEAASKALE-SVDWDSWFYKPGLPP 481

Query: 483 DAYEPVSSLYTSILALANEFKLGK-MPTDEETADWGGQEWELYLQNL---PKSVEASQVQ 542
              E  +SL     ALA +++    +P+  +   W   +  ++LQ +      +  SQ Q
Sbjct: 482 KP-EFDTSLVDKCYALAKKWESKDFVPSPSDIEGWSANQVVVFLQQVQLFTTPLTPSQSQ 541

Query: 543 ALDARYKFSESKNYDVKAAFLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGT 602
           A+   Y   +++N ++ + +  + + +K    Y+     L  VGR+K++R LY  L    
Sbjct: 542 AMGKAYSLVDTQNVELSSRYFGVGLAAKDESVYLPTAELLGKVGRMKFVRTLYRKLL--- 601

Query: 603 GREEEKILAKKIYAEARESYHPIARGVVE 607
               ++ LA++ + + ++ YHPI R  VE
Sbjct: 602 --VVDRKLAEETFEKNKDFYHPICREQVE 603

BLAST of MS010040 vs. ExPASy TrEMBL
Match: A0A6J1C009 (leukotriene A-4 hydrolase homolog OS=Momordica charantia OX=3673 GN=LOC111006242 PE=3 SV=1)

HSP 1 Score: 1213.4 bits (3138), Expect = 0.0e+00
Identity = 610/613 (99.51%), Postives = 610/613 (99.51%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS
Sbjct: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT
Sbjct: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA
Sbjct: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           AIEFSGTEEMVE AEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA
Sbjct: 241 AIEFSGTEEMVEHAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE
Sbjct: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALD RYKFSESKNYDVKAAFLQI
Sbjct: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDGRYKFSESKNYDVKAAFLQI 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQG GREEEKILAKKIYAEARESYHPI
Sbjct: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGPGREEEKILAKKIYAEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           ARGVVESIFAQNM
Sbjct: 601 ARGVVESIFAQNM 613

BLAST of MS010040 vs. ExPASy TrEMBL
Match: A0A6J1JZ81 (leukotriene A-4 hydrolase homolog OS=Cucurbita maxima OX=3661 GN=LOC111489203 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.2e-303
Identity = 513/613 (83.69%), Postives = 564/613 (92.01%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDS+HP T HISLSLFFDF ST IH SALISL++S+SG LSLD RSLTIHS
Sbjct: 1   MAPVDPHSYTDSSHPLTVHISLSLFFDFPSTTIHASALISLSTSHSGPLSLDARSLTIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++DPQS APIPFS++PPD IKGSLLSVSLSGQSSFLVI+STAP+ SALQW+TP QT+NKT
Sbjct: 61  VLDPQSHAPIPFSISPPDPIKGSLLSVSLSGQSSFLVIYSTAPSCSALQWMTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARV YSARLNIP +LSAVMAARSVERR P  GEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVCYSARLNIPRQLSAVMAARSVERRPPDVGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LW G+GRVVEEFVMEQ IPPYLFAFAVGEI F+EVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDFLWGGEGRVVEEFVMEQPIPPYLFAFAVGEIAFKEVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TGA
Sbjct: 241 AREFAGTEEMMRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLN GIGW G +
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNIGIGWNGWK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           +DVERFKDNLEFTKLK NQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI
Sbjct: 361 DDVERFKDNLEFTKLKTNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T LDF+K  +PGIEKE+DL +WTEGTGIPPDAYEPVS+LYT IL+LAN+
Sbjct: 421 ETFKFKSIDTETFLDFLKENLPGIEKEIDLELWTEGTGIPPDAYEPVSNLYTKILSLAND 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP ++ETADWGGQEWELYL+NLPKSVEASQ+QALDARY+ SESK+Y++K AFLQ+
Sbjct: 481 FKLGKMPREDETADWGGQEWELYLENLPKSVEASQIQALDARYRLSESKDYEIKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD YVEVE TL  VGR+KYLRPLY ALTQG+G EE+KILAK+IY+EARESYHPI
Sbjct: 541 AISSKFRDCYVEVEKTLKEVGRMKYLRPLYNALTQGSGMEEDKILAKRIYSEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           A+ VVE+IF++NM
Sbjct: 601 AQRVVEAIFSKNM 613

BLAST of MS010040 vs. ExPASy TrEMBL
Match: A0A6J1GMK0 (leukotriene A-4 hydrolase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455330 PE=3 SV=1)

HSP 1 Score: 1050.8 bits (2716), Expect = 2.2e-303
Identity = 511/613 (83.36%), Postives = 566/613 (92.33%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHS+TDS+HP T HISLSL+FDF ST IHG+AL+SL+SS+SG LSLDTRSLTIH+
Sbjct: 1   MAPVDPHSFTDSSHPLTVHISLSLYFDFPSTTIHGAALVSLSSSHSGPLSLDTRSLTIHA 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++ P+S APIPFSL+PPD IKGSLLSVSLSGQSSFLVI++T+P++SALQWLTP QT+NKT
Sbjct: 61  VLHPESHAPIPFSLSPPDSIKGSLLSVSLSGQSSFLVIYTTSPSSSALQWLTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIP ELSAVMAARSV+RR PVAGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPQELSAVMAARSVKRRPPVAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LWSG+GRVVEEFVMEQ IPPYLFAFAVGEI FREVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDLLWSGEGRVVEEFVMEQPIPPYLFAFAVGEIAFREVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TG 
Sbjct: 241 AREFAGTEEMIRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGV 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFT YAERRIVEVVQGEDAATLN GIGW  L+
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTRYAERRIVEVVQGEDAATLNIGIGWNALK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLK+NQEGVDPDDVYSQIPYEKGFQF+WRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKSNQEGVDPDDVYSQIPYEKGFQFVWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T +DF+KA IPGIEKE+DL MWTEGTGIPPDAYEPVS+LYT IL+LANE
Sbjct: 421 ETFKFKSIDTETFIDFLKANIPGIEKEIDLEMWTEGTGIPPDAYEPVSNLYTKILSLANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP D+ETA WGG+EWELYL+NLPK V+ASQ+QALD RY+FS+SK+Y+VK AFLQ+
Sbjct: 481 FKLGKMPRDDETAAWGGREWELYLENLPKPVDASQIQALDTRYRFSKSKDYEVKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD Y+EVE TL  VGR+KYLRPLY ALTQG G EEEKILAK+IY+EARE YHPI
Sbjct: 541 AISSKFRDCYIEVEKTLKEVGRMKYLRPLYNALTQGPGMEEEKILAKRIYSEARECYHPI 600

Query: 601 ARGVVESIFAQNM 614
           AR VVE++F++NM
Sbjct: 601 ARRVVETVFSKNM 613

BLAST of MS010040 vs. ExPASy TrEMBL
Match: A0A6J1JRM5 (leukotriene A-4 hydrolase homolog OS=Cucurbita maxima OX=3661 GN=LOC111487203 PE=3 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.2e-301
Identity = 508/613 (82.87%), Postives = 563/613 (91.84%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHS+TDS+HP T HISLSL+FDF ST IHG AL+SL++S+SG LSLDTRSLTIHS
Sbjct: 1   MAPVDPHSFTDSSHPLTAHISLSLYFDFPSTTIHGVALVSLSNSHSGPLSLDTRSLTIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++ P+S APIPFSL+PPD IKGSLLSVSLSGQSSFLVI++T+P++SALQWLTP QT+NKT
Sbjct: 61  VLHPESHAPIPFSLSPPDAIKGSLLSVSLSGQSSFLVIYTTSPSSSALQWLTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIP ELSAVMAARSV+RR PVAGEGS 
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPQELSAVMAARSVKRRPPVAGEGSR 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           LAGG   LW G+GRVVEEFVMEQ IPPYLFAFAVGEI FREVGPRTRVY E+ PS LDAA
Sbjct: 181 LAGGVDLLWIGEGRVVEEFVMEQPIPPYLFAFAVGEIAFREVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTEEM+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TG 
Sbjct: 241 AREFAGTEEMIRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGV 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFT YAERRIVEVVQGEDAATLN GIGW  L+
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTRYAERRIVEVVQGEDAATLNIGIGWNALK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           EDVERFKDNLEFTKLK+NQEGVDPDDVYSQ+PYEKGFQF+WRIERQVGRPKFDEFLKKYI
Sbjct: 361 EDVERFKDNLEFTKLKSNQEGVDPDDVYSQVPYEKGFQFVWRIERQVGRPKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T +DF+KA IPGIEKE+DL MWTEGTGIPPDAYEPVS+LYT IL+LANE
Sbjct: 421 ETFKFKSIDTETFIDFLKANIPGIEKEIDLEMWTEGTGIPPDAYEPVSNLYTKILSLANE 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP D+ETA WGG+EWELYL+NLPK V+ASQ+QALDARY+FS+SK+Y+VK AFLQ+
Sbjct: 481 FKLGKMPRDDETAAWGGREWELYLENLPKPVDASQIQALDARYRFSKSKDYEVKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD Y+EVE TL  VGR+KYLRPLY ALTQG G EEEKILAK++Y EARE YHPI
Sbjct: 541 AISSKFRDCYIEVEKTLKEVGRMKYLRPLYNALTQGPGMEEEKILAKRLYCEARECYHPI 600

Query: 601 ARGVVESIFAQNM 614
           AR VVE++F++NM
Sbjct: 601 ARRVVETVFSKNM 613

BLAST of MS010040 vs. ExPASy TrEMBL
Match: A0A6J1FE91 (leukotriene A-4 hydrolase homolog OS=Cucurbita moschata OX=3662 GN=LOC111444926 PE=3 SV=1)

HSP 1 Score: 1045.0 bits (2701), Expect = 1.2e-301
Identity = 510/613 (83.20%), Postives = 565/613 (92.17%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAPVDPHSYTDS+HP T HISLSLFFDF ST IH SALISL++S+SG LSLD RSL IHS
Sbjct: 1   MAPVDPHSYTDSSHPLTVHISLSLFFDFPSTTIHASALISLSTSHSGPLSLDARSLIIHS 60

Query: 61  IVDPQSRAPIPFSLAPPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNKT 120
           ++DPQS+APIPFS++PPD IKGSL+SVSLSGQSSFLVI+STAP+ SALQW+TP QT+NKT
Sbjct: 61  VLDPQSQAPIPFSISPPDSIKGSLVSVSLSGQSSFLVIYSTAPSCSALQWMTPPQTFNKT 120

Query: 121 HPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSM 180
           HPFVYTQCQAIHARSIFPCQDTPAARV +SARLNIP +LSAVMAARSVERR P AGEGSM
Sbjct: 121 HPFVYTQCQAIHARSIFPCQDTPAARVCFSARLNIPRQLSAVMAARSVERRPPGAGEGSM 180

Query: 181 LAGGAASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQLDAA 240
           L GG   LWSG+GRVVEEFVMEQ IPPYLFAFAVGEI F+EVGPRTRVY E+ PS LDAA
Sbjct: 181 LGGGVDLLWSGEGRVVEEFVMEQPIPPYLFAFAVGEIAFKEVGPRTRVYAESVPSVLDAA 240

Query: 241 AIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATGA 300
           A EF+GTE+M+ Q EKL+GPY W+RFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD+TGA
Sbjct: 241 AREFAGTEDMMRQGEKLFGPYDWERFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDSTGA 300

Query: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGWKGLQ 360
           HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLN GIGW G +
Sbjct: 301 HVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNIGIGWNGWK 360

Query: 361 EDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFLKKYI 420
           +DVERFKDNLEFTKLK NQEGVDPDDVYSQIPYEKGFQFLWRIERQVGR KFDEFLKKYI
Sbjct: 361 DDVERFKDNLEFTKLKTNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRLKFDEFLKKYI 420

Query: 421 ETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILALANE 480
           ETFKF+SIDT+T LDF+KA +PGIEKE+DL +WTEGTGIPPDAYEPVS+LYT IL+LAN+
Sbjct: 421 ETFKFKSIDTETFLDFLKANLPGIEKEIDLELWTEGTGIPPDAYEPVSNLYTKILSLAND 480

Query: 481 FKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAAFLQI 540
           FKLGKMP ++ETADWGGQEWELYL+NLPKSVEASQ+QALDARY+ SESK+Y++K AFLQ+
Sbjct: 481 FKLGKMPREDETADWGGQEWELYLENLPKSVEASQIQALDARYRLSESKDYEIKVAFLQL 540

Query: 541 AIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARESYHPI 600
           AI SK+RD YVEVE TL  VGR+KYLRPLY ALTQG+G EE+KILAK+IY+EARESYHPI
Sbjct: 541 AISSKFRDCYVEVEKTLKEVGRMKYLRPLYNALTQGSGMEEDKILAKRIYSEARESYHPI 600

Query: 601 ARGVVESIFAQNM 614
           A+ VVE+IF++NM
Sbjct: 601 AQRVVEAIFSKNM 613

BLAST of MS010040 vs. TAIR 10
Match: AT5G13520.1 (peptidase M1 family protein )

HSP 1 Score: 879.4 bits (2271), Expect = 1.7e-255
Identity = 429/615 (69.76%), Postives = 524/615 (85.20%), Query Frame = 0

Query: 1   MAPVDPHSYTDSTHPFTDHISLSLFFDFSSTIIHGSALISLASSYSGSLSLDTRSLTIHS 60
           MAP+DPHS+TDS+HP T H++LSL+ DF+++IIHGSAL++L+S++SG LSLDTR ++I  
Sbjct: 1   MAPIDPHSFTDSSHPLTTHVALSLYLDFNTSIIHGSALLTLSSAFSGELSLDTRCISIAM 60

Query: 61  IVDPQSRAPIPFSLA-PPDKIKGSLLSVSLSGQSSFLVIFSTAPTASALQWLTPAQTYNK 120
           ++DP +  PIP+S++  PD+I+G+ + V LSGQSS L+++ST+P+ASALQWL+P QT++K
Sbjct: 61  VLDPLTLEPIPYSVSTTPDRIRGTEVVVVLSGQSSLLIVYSTSPSASALQWLSPLQTFSK 120

Query: 121 THPFVYTQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGS 180
            HP+VYTQCQAIHARSIFPCQDTPAAR+RY   +NIP  LSAVM+AR V RR  V  E  
Sbjct: 121 LHPYVYTQCQAIHARSIFPCQDTPAARIRYDVVMNIPNSLSAVMSARHVRRRLAVPEEAK 180

Query: 181 MLAGGA--ASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFREVGPRTRVYGEAAPSQ- 240
            L  G+  +SLW G+ RVVEEF MEQ IPPYLFAFAVGE+ FREVGPRTRVY E+A  + 
Sbjct: 181 HLEAGSLGSSLWCGEDRVVEEFAMEQPIPPYLFAFAVGELGFREVGPRTRVYTESAAIEV 240

Query: 241 LDAAAIEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGD 300
           LDAAA+EF+GTE+M++Q EKL+G Y W+RFDLLVLPPSFPYGGMENP+MVFLTPTVIKGD
Sbjct: 241 LDAAALEFAGTEDMIKQGEKLFGDYEWERFDLLVLPPSFPYGGMENPRMVFLTPTVIKGD 300

Query: 301 ATGAHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNTGIGW 360
           ATGA VVAHELAHSWTGNLITN NNEHFWLNEGFTTYAERRIVEVVQG D ATLN GIGW
Sbjct: 301 ATGAQVVAHELAHSWTGNLITNINNEHFWLNEGFTTYAERRIVEVVQGADIATLNIGIGW 360

Query: 361 KGLQEDVERFKDNLEFTKLKNNQEGVDPDDVYSQIPYEKGFQFLWRIERQVGRPKFDEFL 420
           +GL +++ERFKDNLE TKL N QEGVDPDDVYSQ+PYEKGFQF+ RIERQ+GR  FDEFL
Sbjct: 361 RGLTDEMERFKDNLECTKLWNKQEGVDPDDVYSQVPYEKGFQFVLRIERQIGRTAFDEFL 420

Query: 421 KKYIETFKFQSIDTQTVLDFMKATIPGIEKEVDLVMWTEGTGIPPDAYEPVSSLYTSILA 480
           KKYI TFKF+SIDT T L+F+KA IPGIEKE++L +WTEG GIP DAYEPVS++YT I++
Sbjct: 421 KKYIATFKFKSIDTNTFLEFLKANIPGIEKEINLQLWTEGVGIPEDAYEPVSTIYTKIIS 480

Query: 481 LANEFKLGKMPTDEETADWGGQEWELYLQNLPKSVEASQVQALDARYKFSESKNYDVKAA 540
           LA EFK GKMP++++ A+W GQEWELYL+NLPKS E SQV ALD RY+ +ESK+Y+VK +
Sbjct: 481 LAKEFKEGKMPSEDDVAEWNGQEWELYLENLPKSCEPSQVMALDKRYRLAESKDYEVKVS 540

Query: 541 FLQIAIVSKYRDSYVEVENTLTAVGRIKYLRPLYTALTQGTGREEEKILAKKIYAEARES 600
           FLQ+A+ SK R+ + EV+ TL  VGR+KYLRPL+TAL Q +G  EEK LAK+++AEARE+
Sbjct: 541 FLQLAVTSKCREYHGEVKKTLKEVGRMKYLRPLFTALAQ-SGGTEEKQLAKQVFAEARET 600

Query: 601 YHPIARGVVESIFAQ 612
           YHPIA+GVVESI ++
Sbjct: 601 YHPIAQGVVESILSK 614

BLAST of MS010040 vs. TAIR 10
Match: AT4G33090.1 (aminopeptidase M1 )

HSP 1 Score: 99.0 bits (245), Expect = 1.4e-20
Identity = 90/357 (25.21%), Postives = 144/357 (40.34%), Query Frame = 0

Query: 126 TQCQAIHARSIFPCQDTPAARVRYSARLNIPGELSAVMAARSVERRSPVAGEGSMLAGGA 185
           TQ +   AR  FPC D PA +  +   L +P +L A+     +E +              
Sbjct: 135 TQFEPADARRCFPCWDEPACKATFKITLEVPTDLVALSNMPIMEEKV------------- 194

Query: 186 ASLWSGKGRVVEEFVMEQTIPPYLFAFAVGEIDFRE----VGPRTRVYGEAAPSQLDAAA 245
               +G  ++V  +     +  YL A  VG  D+ E     G + RVY +   +     A
Sbjct: 195 ----NGNLKIV-SYQESPIMSTYLVAIVVGLFDYVEDHTSDGIKVRVYCQVGKADQGKFA 254

Query: 246 IEFSGTEEMVEQAEKLYGPYVWDRFDLLVLPPSFPYGGMENPKMVFLTPTVIKGDATG-- 305
           +   G + +    E    PY   + D++ + P F  G MEN  +V    T +  D     
Sbjct: 255 LHV-GAKTLDLFKEYFAVPYPLPKMDMIAI-PDFAAGAMENYGLVTYRETALLYDEQHSA 314

Query: 306 -------AHVVAHELAHSWTGNLITNKNNEHFWLNEGFTTYAERRIVEVVQGEDAATLNT 365
                  A VVAHELAH W GNL+T +   H WLNEGF T+      + +  E       
Sbjct: 315 ASNKQRVATVVAHELAHQWFGNLVTMEWWTHLWLNEGFATWVSYLATDSLFPE------- 374

Query: 366 GIGWKGLQEDVERFKDNLEFTKLKNN-------QEGVDPDDVYSQIPYEKGFQFLWRIER 425
              WK   + ++   + L    L+ +           + D+++  I Y KG   +  ++ 
Sbjct: 375 ---WKIWTQFLDESTEGLRLDGLEESHPIEVEVNHAAEIDEIFDAISYRKGASVIRMLQS 434

Query: 426 QVGRPKFDEFLKKYIETFKFQSIDTQTVLDFMKATIPGIEKEVDLVM--WTEGTGIP 461
            +G   F + L  YI+   + +  T+   D   A   G  + V+ +M  WT+  G P
Sbjct: 435 YLGAEVFQKSLAAYIKNHAYSNAKTE---DLWAALEAGSGEPVNKLMSSWTKQKGYP 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022133728.10.0e+0099.51leukotriene A-4 hydrolase homolog [Momordica charantia][more]
XP_022993079.14.5e-30383.69leukotriene A-4 hydrolase homolog [Cucurbita maxima][more]
KAG7033020.14.5e-30383.36Leukotriene A-4 hydrolase-like protein [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022952720.14.5e-30383.36leukotriene A-4 hydrolase homolog [Cucurbita moschata][more]
KAG6578737.18.5e-30283.20Leucine aminopeptidase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9FY492.4e-25469.76Leucine aminopeptidase OS=Arabidopsis thaliana OX=3702 GN=LKHA4 PE=2 SV=1[more]
Q84TA31.1e-22463.41Leucine aminopeptidase OS=Oryza sativa subsp. japonica OX=39947 GN=LKHA4 PE=2 SV... [more]
P099603.0e-10037.11Leukotriene A-4 hydrolase OS=Homo sapiens OX=9606 GN=LTA4H PE=1 SV=2[more]
Q7S7853.9e-10038.41Leucine aminopeptidase 2 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / ... [more]
A7EJL96.7e-10036.72Leucine aminopeptidase 2 OS=Sclerotinia sclerotiorum (strain ATCC 18683 / 1980 /... [more]
Match NameE-valueIdentityDescription
A0A6J1C0090.0e+0099.51leukotriene A-4 hydrolase homolog OS=Momordica charantia OX=3673 GN=LOC111006242... [more]
A0A6J1JZ812.2e-30383.69leukotriene A-4 hydrolase homolog OS=Cucurbita maxima OX=3661 GN=LOC111489203 PE... [more]
A0A6J1GMK02.2e-30383.36leukotriene A-4 hydrolase homolog OS=Cucurbita moschata OX=3662 GN=LOC111455330 ... [more]
A0A6J1JRM51.2e-30182.87leukotriene A-4 hydrolase homolog OS=Cucurbita maxima OX=3661 GN=LOC111487203 PE... [more]
A0A6J1FE911.2e-30183.20leukotriene A-4 hydrolase homolog OS=Cucurbita moschata OX=3662 GN=LOC111444926 ... [more]
Match NameE-valueIdentityDescription
AT5G13520.11.7e-25569.76peptidase M1 family protein [more]
AT4G33090.11.4e-2025.21aminopeptidase M1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001930Peptidase M1, alanine aminopeptidase/leukotriene A4 hydrolasePRINTSPR00756ALADIPTASEcoord: 134..149
score: 41.85
coord: 321..333
score: 52.2
coord: 275..285
score: 49.68
coord: 302..317
score: 66.52
IPR015211Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminalSMARTSM01263Leuk_A4_hydro_C_2coord: 469..609
e-value: 2.0E-39
score: 147.0
IPR015211Peptidase M1, leukotriene A4 hydrolase/aminopeptidase C-terminalPFAMPF09127Leuk-A4-hydro_Ccoord: 495..608
e-value: 2.0E-26
score: 92.4
NoneNo IPR availableGENE3D3.30.2010.30coord: 216..314
e-value: 9.3E-40
score: 136.5
NoneNo IPR availablePFAMPF17900Peptidase_M1_Ncoord: 18..162
e-value: 1.6E-13
score: 51.3
NoneNo IPR availablePANTHERPTHR45726:SF3LEUKOTRIENE A-4 HYDROLASEcoord: 2..609
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 227..460
IPR042097Aminopeptidase N-like , N-terminalGENE3D2.60.40.1730tricorn interacting facor f3 domaincoord: 17..215
e-value: 9.4E-34
score: 119.4
IPR042097Aminopeptidase N-like , N-terminalSUPERFAMILY63737Leukotriene A4 hydrolase N-terminal domaincoord: 3..216
IPR038502Peptidase M1, LTA-4 hydrolase/aminopeptidase, C-terminal domain superfamilyGENE3D1.25.40.320coord: 468..609
e-value: 1.4E-36
score: 127.2
IPR014782Peptidase M1, membrane alanine aminopeptidasePFAMPF01433Peptidase_M1coord: 249..450
e-value: 1.8E-42
score: 145.4
IPR027268Peptidase M4/M1, CTD superfamilyGENE3D1.10.390.10Neutral Protease Domain 2coord: 315..464
e-value: 8.7E-35
score: 122.3
IPR034015Aminopeptidase, leukotriene A4 hydrolase-likePANTHERPTHR45726FAMILY NOT NAMEDcoord: 2..609
IPR034015Aminopeptidase, leukotriene A4 hydrolase-likeCDDcd09599M1_LTA4Hcoord: 5..453
e-value: 0.0
score: 609.073
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 486..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS010040.1MS010040.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0008237 metallopeptidase activity
molecular_function GO:0008270 zinc ion binding