MS009864 (gene) Bitter gourd (TR) v1

Overview
NameMS009864
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionBasic helix-loop-helix transcription factor
Locationscaffold173: 933286 .. 936106 (+)
RNA-Seq ExpressionMS009864
SyntenyMS009864
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTCCCAAGCCCACCGGAATCCTCTGGATCGACGGCGACCACGACGACGCCGCCTCTTGGACCAGAAACAACAACGACAACGACGACGACATCGCCATTGCCGGCTCCATTTCTTCCTTCAAATCCATGCTCGAACCCGACTGGTACATCAACTCCTCTACTCCTCTCAATCCCTCCTCTCTCCAAGATTTCCATTCCCTCTCTTCTGCTTCTCACCACCACATTCCGGACCTTGCTTTCTCCTCCAATCCTTCTGATAACTTGCTCTTGAACCCCATAGATTCCTCCTCTTCCTGCTCTCCCTCCCAGGCTTTTGCTCTCGATCCTTCGCATTCCCAATCCCAATCCATGTTGCCTCCTCATAAATCGTGTTTCTCTTCTCTTCTTAATGTCGTTTGCGCCAGCAATTTTGATAATGGCTTCGATTTGGGCGGCGACACTGGCCTGCTTGGATCTTACCAGGGGAATCCAGCTTCCAATTCCCCCGTTTTGATGGGCTTCACCGGCATTTCGTCTCTACCTCAAATCGGTAATCAGGAAATGAGCTTAAACAACTCCGATTTTCCGCCGGGCCGGTTGCTTCCGGTCGTTGATAACCCTCCCGCGTTCGGAGCTGGCTTTAATCCGGCGGCTGCCTTTGAGAGTTTCGATGGCTGTGGGAATGCTCTGTTTCAGAACAGGTCTAAGGTTTTGAGACCTCTCGAAGTGTTTCCCCAGGTCGGCACACAGCCGACGCTGTTCCAGAAGCGAGCGGCGCACCGCGTTGGCTCCGCCGGAGCTGATAAACTGGAGAATTTGGAGGTTTCGGATATGAAATTAGGAGAAGGCTCAATGTGGACGGCAAATTTGGAGAAGCTGAGGAATAAGAACGGCGAAGAGGATATTGAGGACGGCAGCGTGGACGTTTCTCGCTTCAATTATGATTCCGACGAACCCAATGAGTACAATAAGCAAGAGGACAACATTAACACAAAAAACTGCGGAAGCAATTCCAATGCCAATAGTACTGTAACTGGCGGAGACCAGAAGGGGAAGAAGAAGGGGCTGCCGGCAAAAAATCTCATGGCGGAGCGGCGGCGCAGGAAGAAGCTCAACGATAGGCTTTACATGCTTAGGTCCGTCGTACCCAAAATAAGCAAGGTATGGATAGATCTTGAACATGTTTTCTTTGAGATTACAATTGAAAGTTTTTAGCTATTTACGATTCAGAATAATTTGGATTTATGGCATAATGTATCTAATTTATGATCGTTAAGCTGTAATCTTTCTTGGGGAAGTGGGCAATAACTCCTGTTGTCTCATCGTTTAATTTCTTTCTGATAATCCTCTGTGAAGCCAGACCTCAAAATTGTGTTTTCCTTGTTTGCACAGATGGATAGAGCTTCAATACTTGGAGATGCCATTGATTACTTGAAGGAGCTTCTCCAAAGGATAAATGATCTTCACAATGAACTTGAGTCAACTCCTGGTTCTCTGTTGCAGCCTTCTGCGAGCTTTCACCCGTTGACGCCGACGCCGCCGACTCTTCCCTGTCGGGTCAAAGAAGAGCTGTGTCCAAGCTCCTTGTCCAGCCCTAAAACCCAGCCTGCCAGGGTAAATATAATCTAACTTCCATCTTTCTTTCTTTTTCATTCTGCTGTTTGTTTTTGTTTCTTCTACGGTTTGATAGTATCTATGATGCAGCTATAATTTCTCTCTTTTTGTTTGTTTTTAAACTTTCGTAACAAGAACATGTAGCAGGAGGTGGGATTTGGTTATGTAGTGTTAAGGGCATACAATTCTTCTTATATTTGTGATGCAAAAGATTGCAAAGTGATTTTTCTTTTTTTTTTTTAACTGTGGTTGGATGTAGAAGCTACCAATGCATGAACAAATTTTGCCTTGTTCGAATGAGTTAATTCTGGAATTTAATTTTTGAAATTTCAATTTGTTCTTCAGTCAAATGAATCAGTATGAAGTCCCCCAAATAATGAGGTCCAATGATCTATCTGATACTTGCATGGTATAATTTCTTAAACTAGCAATAGCAATGGTGTGTAACAGTCTCATCGTCCTATTTTTTTAGGCTTTAGAAAATATGACCAATTTTGGCAGAGTAGACAGAGGCCCAAGAATGAGTCGTGAATGGATTTGTTCAACAGACCTTGTAGATTTGTTGTTAGTTCTAATGACATTGGTGGAGCTCATACATCTGTTTTTATCTTACTAATTCCATCCATGGAAGTCGGTTCGACCGGGTTCTGATCATTTGACAAACTCAAGCACAGTTTCTATCCATTTTCTGTGAATTTCCATATAAAGTTGGTTTCATTCTTGAACGCTTGTGGGATTTATTTGCATTGTATTCTATGATGAAGATCTGCTTCCAAGTTCCAATCATGATTTGTATGGCGTATAAATAACAGGTTGAAGTCCGGGTCAGAGAAGGCAGAGCTGTCAACATTCACATGTTTTGTTCTCGCAAGCCGGGTCTCTTGCTATCCACCATGAGAGCTCTCGATAACCTCGGGCTGGACGTCCAACAAGCAGTGATCAGCTGTTTCAATGGATTTGCTTTGGATGTGTTTCGAGCTGAGGTTTGCTCTCTTTTTCATTTAAGTTGTAGAGCATTCATTCATGTATGATTATTATCTGCTTTTGGTACTCACCGTGTCGTGTCTTTCTCTACGGCTGTTCGATTTCGTCCATGTTTGTCTCCATTTTTCCTGCAAGGACTCACTCCTTCTCTCTGTTTCAGCAATCCAAGGAAGGTCAGGAGATCTTACCTGAGCAAATTAAAGCAGTGCTATTGGATTCAGCTGGTTTACATGGTGTGATT

mRNA sequence

ATGCTTCCCAAGCCCACCGGAATCCTCTGGATCGACGGCGACCACGACGACGCCGCCTCTTGGACCAGAAACAACAACGACAACGACGACGACATCGCCATTGCCGGCTCCATTTCTTCCTTCAAATCCATGCTCGAACCCGACTGGTACATCAACTCCTCTACTCCTCTCAATCCCTCCTCTCTCCAAGATTTCCATTCCCTCTCTTCTGCTTCTCACCACCACATTCCGGACCTTGCTTTCTCCTCCAATCCTTCTGATAACTTGCTCTTGAACCCCATAGATTCCTCCTCTTCCTGCTCTCCCTCCCAGGCTTTTGCTCTCGATCCTTCGCATTCCCAATCCCAATCCATGTTGCCTCCTCATAAATCGTGTTTCTCTTCTCTTCTTAATGTCGTTTGCGCCAGCAATTTTGATAATGGCTTCGATTTGGGCGGCGACACTGGCCTGCTTGGATCTTACCAGGGGAATCCAGCTTCCAATTCCCCCGTTTTGATGGGCTTCACCGGCATTTCGTCTCTACCTCAAATCGGTAATCAGGAAATGAGCTTAAACAACTCCGATTTTCCGCCGGGCCGGTTGCTTCCGGTCGTTGATAACCCTCCCGCGTTCGGAGCTGGCTTTAATCCGGCGGCTGCCTTTGAGAGTTTCGATGGCTGTGGGAATGCTCTGTTTCAGAACAGGTCTAAGGTTTTGAGACCTCTCGAAGTGTTTCCCCAGGTCGGCACACAGCCGACGCTGTTCCAGAAGCGAGCGGCGCACCGCGTTGGCTCCGCCGGAGCTGATAAACTGGAGAATTTGGAGGTTTCGGATATGAAATTAGGAGAAGGCTCAATGTGGACGGCAAATTTGGAGAAGCTGAGGAATAAGAACGGCGAAGAGGATATTGAGGACGGCAGCGTGGACGTTTCTCGCTTCAATTATGATTCCGACGAACCCAATGAGTACAATAAGCAAGAGGACAACATTAACACAAAAAACTGCGGAAGCAATTCCAATGCCAATAGTACTGTAACTGGCGGAGACCAGAAGGGGAAGAAGAAGGGGCTGCCGGCAAAAAATCTCATGGCGGAGCGGCGGCGCAGGAAGAAGCTCAACGATAGGCTTTACATGCTTAGGTCCGTCGTACCCAAAATAAGCAAGATGGATAGAGCTTCAATACTTGGAGATGCCATTGATTACTTGAAGGAGCTTCTCCAAAGGATAAATGATCTTCACAATGAACTTGAGTCAACTCCTGGTTCTCTGTTGCAGCCTTCTGCGAGCTTTCACCCGTTGACGCCGACGCCGCCGACTCTTCCCTGTCGGGTCAAAGAAGAGCTGTGTCCAAGCTCCTTGTCCAGCCCTAAAACCCAGCCTGCCAGGGTTGAAGTCCGGGTCAGAGAAGGCAGAGCTGTCAACATTCACATGTTTTGTTCTCGCAAGCCGGGTCTCTTGCTATCCACCATGAGAGCTCTCGATAACCTCGGGCTGGACGTCCAACAAGCAGTGATCAGCTGTTTCAATGGATTTGCTTTGGATGTGTTTCGAGCTGAGCAATCCAAGGAAGGTCAGGAGATCTTACCTGAGCAAATTAAAGCAGTGCTATTGGATTCAGCTGGTTTACATGGTGTGATT

Coding sequence (CDS)

ATGCTTCCCAAGCCCACCGGAATCCTCTGGATCGACGGCGACCACGACGACGCCGCCTCTTGGACCAGAAACAACAACGACAACGACGACGACATCGCCATTGCCGGCTCCATTTCTTCCTTCAAATCCATGCTCGAACCCGACTGGTACATCAACTCCTCTACTCCTCTCAATCCCTCCTCTCTCCAAGATTTCCATTCCCTCTCTTCTGCTTCTCACCACCACATTCCGGACCTTGCTTTCTCCTCCAATCCTTCTGATAACTTGCTCTTGAACCCCATAGATTCCTCCTCTTCCTGCTCTCCCTCCCAGGCTTTTGCTCTCGATCCTTCGCATTCCCAATCCCAATCCATGTTGCCTCCTCATAAATCGTGTTTCTCTTCTCTTCTTAATGTCGTTTGCGCCAGCAATTTTGATAATGGCTTCGATTTGGGCGGCGACACTGGCCTGCTTGGATCTTACCAGGGGAATCCAGCTTCCAATTCCCCCGTTTTGATGGGCTTCACCGGCATTTCGTCTCTACCTCAAATCGGTAATCAGGAAATGAGCTTAAACAACTCCGATTTTCCGCCGGGCCGGTTGCTTCCGGTCGTTGATAACCCTCCCGCGTTCGGAGCTGGCTTTAATCCGGCGGCTGCCTTTGAGAGTTTCGATGGCTGTGGGAATGCTCTGTTTCAGAACAGGTCTAAGGTTTTGAGACCTCTCGAAGTGTTTCCCCAGGTCGGCACACAGCCGACGCTGTTCCAGAAGCGAGCGGCGCACCGCGTTGGCTCCGCCGGAGCTGATAAACTGGAGAATTTGGAGGTTTCGGATATGAAATTAGGAGAAGGCTCAATGTGGACGGCAAATTTGGAGAAGCTGAGGAATAAGAACGGCGAAGAGGATATTGAGGACGGCAGCGTGGACGTTTCTCGCTTCAATTATGATTCCGACGAACCCAATGAGTACAATAAGCAAGAGGACAACATTAACACAAAAAACTGCGGAAGCAATTCCAATGCCAATAGTACTGTAACTGGCGGAGACCAGAAGGGGAAGAAGAAGGGGCTGCCGGCAAAAAATCTCATGGCGGAGCGGCGGCGCAGGAAGAAGCTCAACGATAGGCTTTACATGCTTAGGTCCGTCGTACCCAAAATAAGCAAGATGGATAGAGCTTCAATACTTGGAGATGCCATTGATTACTTGAAGGAGCTTCTCCAAAGGATAAATGATCTTCACAATGAACTTGAGTCAACTCCTGGTTCTCTGTTGCAGCCTTCTGCGAGCTTTCACCCGTTGACGCCGACGCCGCCGACTCTTCCCTGTCGGGTCAAAGAAGAGCTGTGTCCAAGCTCCTTGTCCAGCCCTAAAACCCAGCCTGCCAGGGTTGAAGTCCGGGTCAGAGAAGGCAGAGCTGTCAACATTCACATGTTTTGTTCTCGCAAGCCGGGTCTCTTGCTATCCACCATGAGAGCTCTCGATAACCTCGGGCTGGACGTCCAACAAGCAGTGATCAGCTGTTTCAATGGATTTGCTTTGGATGTGTTTCGAGCTGAGCAATCCAAGGAAGGTCAGGAGATCTTACCTGAGCAAATTAAAGCAGTGCTATTGGATTCAGCTGGTTTACATGGTGTGATT

Protein sequence

MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLPPHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGLHGVI
Homology
BLAST of MS009864 vs. NCBI nr
Match: XP_038893918.1 (transcription factor ICE1 [Benincasa hispida])

HSP 1 Score: 887.1 bits (2291), Expect = 7.6e-254
Identity = 475/552 (86.05%), Postives = 493/552 (89.31%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAI-AGSISSFKSMLEPDWYINS--STPL 60
           MLPK TGILW+DGDHDDA SWT  N+ NDD IAI A SISSFKSMLE DWYINS  +TPL
Sbjct: 81  MLPKSTGILWLDGDHDDATSWTTTNHHNDDPIAIAAASISSFKSMLESDWYINSTTTTPL 140

Query: 61  NPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQS 120
           NPS  QDFHSLS  SH  IP L FSSNPSDN LL+PIDSSSSCSPS AF LDP+H  S S
Sbjct: 141 NPSH-QDFHSLS--SHPDIPHLPFSSNPSDNFLLHPIDSSSSCSPSHAFPLDPAH--SHS 200

Query: 121 MLPPHK-SCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQ 180
           +LP HK SCFSSLLNVVC SNFDN FDLGG++GLLG YQGN ASNS VLMGFTGISSLPQ
Sbjct: 201 ILPSHKSSCFSSLLNVVCGSNFDNAFDLGGESGLLGPYQGNQASNSSVLMGFTGISSLPQ 260

Query: 181 IGNQ-------EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRS 240
           IGNQ       E+SLNNSDFP  RLLPVVDNP AFGAGFNPAAAFESFD  GNALFQNRS
Sbjct: 261 IGNQELSLNNSELSLNNSDFPATRLLPVVDNPLAFGAGFNPAAAFESFDDSGNALFQNRS 320

Query: 241 KVLRPLEVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRN 300
           KVL+PLEVFPQVG QPTL+QKRAAHRVGSAG DKLENLEVS  KLGEGSMWT NLE+LR 
Sbjct: 321 KVLKPLEVFPQVGAQPTLYQKRAAHRVGSAGPDKLENLEVSGFKLGEGSMWTPNLERLRK 380

Query: 301 KNGEEDIEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKG 360
             G+EDIEDGSVDVSR NYDSDEPNE+NKQEDN+N KNCGSNSNANST+TGGDQKGKKKG
Sbjct: 381 MTGDEDIEDGSVDVSRLNYDSDEPNEHNKQEDNVNAKNCGSNSNANSTITGGDQKGKKKG 440

Query: 361 LPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNEL 420
           LPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNEL
Sbjct: 441 LPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNEL 500

Query: 421 EST-PGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPK-TQPARVEVRVREGRAVN 480
           EST PGSLLQPSASFHPLTPT PTLPCRVKEELCPSSLSSP  TQPARVEVRVREGRAVN
Sbjct: 501 ESTPPGSLLQPSASFHPLTPTLPTLPCRVKEELCPSSLSSPNTTQPARVEVRVREGRAVN 560

Query: 481 IHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKA 540
           IHMFCSRKPGLLLSTMRALDNLGLD+QQAVISCFNGFALD+F AEQSKEGQEILPEQIKA
Sbjct: 561 IHMFCSRKPGLLLSTMRALDNLGLDIQQAVISCFNGFALDIFSAEQSKEGQEILPEQIKA 620

BLAST of MS009864 vs. NCBI nr
Match: XP_011651587.1 (transcription factor ICE1 [Cucumis sativus] >KGN58244.1 hypothetical protein Csa_017648 [Cucumis sativus])

HSP 1 Score: 850.5 bits (2196), Expect = 7.9e-243
Identity = 454/544 (83.46%), Postives = 479/544 (88.05%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASW-TRNNNDNDDDIAI--AGSISSFKSMLEPDWYINSSTPL 60
           MLPK TGILW+DGDH+DA SW T  N+DN   + +  A SISSFKS+L+ D YINSSTPL
Sbjct: 1   MLPKSTGILWLDGDHEDATSWITTTNHDNHHSLPVPPAPSISSFKSILQSDCYINSSTPL 60

Query: 61  NPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQS 120
           NPS  QDFHSLS  SH HIPDL FSSNPSDN LL+P+DSSSSCSPS AF L PSH  S S
Sbjct: 61  NPSH-QDFHSLS--SHPHIPDLPFSSNPSDNFLLHPLDSSSSCSPSHAFPLHPSH--SNS 120

Query: 121 MLPPHK-SCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQ 180
           +LP HK SCFSSLLNVVC S+FDN FDLGG+ GLLGSYQGN A     LMGFTGISSLPQ
Sbjct: 121 ILPSHKSSCFSSLLNVVCGSSFDNAFDLGGENGLLGSYQGNQA-----LMGFTGISSLPQ 180

Query: 181 IGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLE 240
           I  QE++LNNSDF       V DNPPAFGAGFN AAAFE+FD CGNALFQNRSKVLRPLE
Sbjct: 181 IATQELTLNNSDF------HVPDNPPAFGAGFNSAAAFENFDACGNALFQNRSKVLRPLE 240

Query: 241 VFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDI 300
           VFP+VG QPTLFQKRAAHRVGS GADKLENLEVS  KL EG MWT NLE+LR  NG+EDI
Sbjct: 241 VFPEVGAQPTLFQKRAAHRVGSGGADKLENLEVSGFKLEEGPMWTPNLERLRKMNGDEDI 300

Query: 301 EDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLM 360
           EDGSVDVSRFNYDSDEP EYNKQEDN+N KNCGSNSNANST+TGGDQKGKKKGLPAKNLM
Sbjct: 301 EDGSVDVSRFNYDSDEPIEYNKQEDNVNAKNCGSNSNANSTITGGDQKGKKKGLPAKNLM 360

Query: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PGS 420
           AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLH ELEST PGS
Sbjct: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHKELESTPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
           +LQPSASFHPLTPT PTLPCRVKEELCPS+LSSP TQPARVEVRVREGRAVNIHMFCSRK
Sbjct: 421 VLQPSASFHPLTPTLPTLPCRVKEELCPSNLSSPNTQPARVEVRVREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTMRALDNLGLD+QQAVISCFNGFALDVFRAEQSKEGQE+LPEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMRALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGQEMLPEQIKAVLLDSAGL 528

BLAST of MS009864 vs. NCBI nr
Match: XP_008450018.1 (PREDICTED: transcription factor ICE1 [Cucumis melo])

HSP 1 Score: 841.3 bits (2172), Expect = 4.8e-240
Identity = 451/544 (82.90%), Postives = 476/544 (87.50%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASW-TRNNNDNDDDIAI--AGSISSFKSMLEPDWYINSSTPL 60
           MLPK TGILW+DGDH+DA SW T  N+DN   + I  A SISSFKS+L  D YINSSTPL
Sbjct: 1   MLPKSTGILWLDGDHEDATSWTTTTNHDNHHSLPIPPAPSISSFKSILPSDCYINSSTPL 60

Query: 61  NPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQS 120
           NPS  QDF SLS  SH H+PDL FSSNPSD+ LL+P+DSSSSCSPS AF L PSH  S S
Sbjct: 61  NPSH-QDFPSLS--SHPHLPDLPFSSNPSDDFLLHPLDSSSSCSPSHAFPLHPSH--SNS 120

Query: 121 MLPPHK-SCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQ 180
           +LP HK SCFSSLLNVVC S+FDN FDLGG+ GLLGSYQGN A     LMGFTGISSLPQ
Sbjct: 121 ILPSHKSSCFSSLLNVVCGSSFDNAFDLGGENGLLGSYQGNQA-----LMGFTGISSLPQ 180

Query: 181 IGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLE 240
           I NQE++LNNSDF      PV DN PAFG GFN AAAFE+FD CGNALFQNRSKVLRPLE
Sbjct: 181 IANQELTLNNSDF------PVPDNSPAFGPGFNSAAAFENFDACGNALFQNRSKVLRPLE 240

Query: 241 VFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDI 300
           VFP+VG QPTLFQKRAAHRVGS G DKLENLEVS  KLGEGSMWT NLE+LR  NG+EDI
Sbjct: 241 VFPEVGVQPTLFQKRAAHRVGSGGPDKLENLEVSGFKLGEGSMWTPNLERLRKMNGDEDI 300

Query: 301 EDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLM 360
           EDGSVDVSR NYDSDEP EYNKQEDN+N K CGS+SNANST+TGGDQKGKKKGLPAKNLM
Sbjct: 301 EDGSVDVSRLNYDSDEPIEYNKQEDNVNAKKCGSHSNANSTITGGDQKGKKKGLPAKNLM 360

Query: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PGS 420
           AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PGS
Sbjct: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
           +LQPSASFHPLTPT PTLPCRVKEELCPS+LSSP  QPARVEVRVREGRAVNIHMFCSRK
Sbjct: 421 VLQPSASFHPLTPTLPTLPCRVKEELCPSNLSSPYAQPARVEVRVREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTMRALDNLGLD+QQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMRALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 528

BLAST of MS009864 vs. NCBI nr
Match: XP_022983584.1 (transcription factor ICE1-like [Cucurbita maxima])

HSP 1 Score: 835.9 bits (2158), Expect = 2.0e-238
Identity = 434/544 (79.78%), Postives = 473/544 (86.95%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPS 60
           MLPK TGILW+DGDH D ASW R NN N+DDIA+AGS SSFKSML+ DWY+NS+TPLNPS
Sbjct: 1   MLPKSTGILWLDGDHHDGASWNRTNNHNNDDIAVAGSASSFKSMLQSDWYMNSTTPLNPS 60

Query: 61  SLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLP 120
           + QDFHSLS  SH  + +L F+SNP DNLLL PIDSSS+CS SQAF+LDPSH  SQSMLP
Sbjct: 61  N-QDFHSLS--SHPDVRNLPFNSNPPDNLLLRPIDSSSACSASQAFSLDPSH--SQSMLP 120

Query: 121 PHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQ 180
           P KSCF SL NV C+SNF+NGFDLGG+ GLLGSYQGN  SNS  +MGF GISSLPQIGNQ
Sbjct: 121 PQKSCFPSLFNVACSSNFENGFDLGGENGLLGSYQGNQVSNSSAMMGFNGISSLPQIGNQ 180

Query: 181 EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQ 240
           E+SLNN++F      PV DNPPAFGAGFNP  AFESFDGCGNALFQNRSK LRP E FPQ
Sbjct: 181 ELSLNNAEF------PVADNPPAFGAGFNPTDAFESFDGCGNALFQNRSKALRPPESFPQ 240

Query: 241 V-GTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDG 300
           V GTQPTLFQKRAAHRVGS+GADKLENLEVS  K G+GSMW  N+E+L  KN +EDIED 
Sbjct: 241 VGGTQPTLFQKRAAHRVGSSGADKLENLEVSGSKFGDGSMWMPNMERLM-KNSDEDIEDD 300

Query: 301 SVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAER 360
           S+D+S  NYDSDE NEYNK EDN+N KN GSNSNAN+TV GG+QKGK+KG+PAKNLMAER
Sbjct: 301 SLDISHLNYDSDEANEYNKLEDNVNAKNSGSNSNANTTVAGGNQKGKRKGMPAKNLMAER 360

Query: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST----PGS 420
           RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQ+IN+LHNELEST    PGS
Sbjct: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQKINELHNELESTPPPPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
            LQP ASFHP TPT P LPCRVK+ELCPSS+SSPK QPARVEVR REGRAVNIHMFCSRK
Sbjct: 421 SLQPPASFHPSTPTLPNLPCRVKDELCPSSMSSPKAQPARVEVRAREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTM+ALDNLGLDVQQAV+SCFNGFALDVFRAEQSKEG+EI PEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMKALDNLGLDVQQAVVSCFNGFALDVFRAEQSKEGEEISPEQIKAVLLDSAGL 532

BLAST of MS009864 vs. NCBI nr
Match: XP_022934114.1 (transcription factor ICE1-like [Cucurbita moschata])

HSP 1 Score: 833.2 bits (2151), Expect = 1.3e-237
Identity = 437/551 (79.31%), Postives = 474/551 (86.03%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPS 60
           MLPK TGILW+DGDH D ASW R NN N+DDIA+AGS SSFKSML+ DWY+NS+TPLNPS
Sbjct: 1   MLPKSTGILWLDGDHHDGASWNRTNNHNNDDIAVAGSASSFKSMLQSDWYMNSTTPLNPS 60

Query: 61  SLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLP 120
           + QDFHSLS  SH  + DL F+SNP DNLLL PIDSSS+CS SQAFALDPSH  SQSMLP
Sbjct: 61  N-QDFHSLS--SHPDVRDLPFNSNPPDNLLLRPIDSSSACSASQAFALDPSH--SQSMLP 120

Query: 121 PHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQ 180
           P KSCF SL NV C+SNF+NGFDLGG+ GLLGSYQGN  SNS  +MGF GISSLPQIGNQ
Sbjct: 121 PQKSCFPSLYNVACSSNFENGFDLGGENGLLGSYQGNQVSNSSGMMGFNGISSLPQIGNQ 180

Query: 181 EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQ 240
           E+SLNN++F      PV DNPPAFGAGFNP  AFESFDGCGNALFQNRSK LRP E FPQ
Sbjct: 181 ELSLNNAEF------PVADNPPAFGAGFNPTDAFESFDGCGNALFQNRSKALRPPESFPQ 240

Query: 241 V-GTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDG 300
           V GTQPTLFQKRAAHRVGS+G DKLENLEVS  K G+GSMW  N+E+L  KN +EDIED 
Sbjct: 241 VGGTQPTLFQKRAAHRVGSSGVDKLENLEVSGSKFGDGSMWMPNMERLM-KNSDEDIEDD 300

Query: 301 SVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAER 360
           S+D+SR NYDSDE NEYNK EDN+N KN GSNSNAN+TVT G+QKGK+KG+PAKNLMAER
Sbjct: 301 SLDISRLNYDSDEANEYNKLEDNVNAKNSGSNSNANTTVTDGNQKGKRKGMPAKNLMAER 360

Query: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST------P 420
           RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQ+IN+LHNELEST      P
Sbjct: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQKINELHNELESTPPPPPPP 420

Query: 421 GSLLQPSASFHPLTPT-----PPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNI 480
           GS LQP ASFHPLTPT      P LPCRVKEELCPSS+SSPK QPARVEVR REGRAVNI
Sbjct: 421 GSSLQPPASFHPLTPTLPIPMLPNLPCRVKEELCPSSMSSPKAQPARVEVRAREGRAVNI 480

Query: 481 HMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAV 540
           HMFCSRKPGLLLSTM+ALDNLGLD+QQAVISCFNGFALDVFRAEQSKEG+E+ PEQIKAV
Sbjct: 481 HMFCSRKPGLLLSTMKALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGEEVSPEQIKAV 539

BLAST of MS009864 vs. ExPASy Swiss-Prot
Match: Q9LSE2 (Transcription factor ICE1 OS=Arabidopsis thaliana OX=3702 GN=SCRM PE=1 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 8.7e-128
Identity = 297/545 (54.50%), Postives = 346/545 (63.49%), Query Frame = 0

Query: 7   GILWIDG-----DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 66
           G +W++G     + ++  SW RN  D           S FK MLE DW+  SS   +P  
Sbjct: 9   GGVWLNGGGGEREENEEGSWGRNQEDGS---------SQFKPMLEGDWF--SSNQPHPQD 68

Query: 67  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL-NPIDSSSSCSPSQAFALDPSHSQSQSMLP 126
           LQ   +     +       F  NP+DNLLL + IDSSSSCSPSQAF+LDPS         
Sbjct: 69  LQMLQNQPDFRYFG----GFPFNPNDNLLLQHSIDSSSSCSPSQAFSLDPSQQNQFLSTN 128

Query: 127 PHKSCFSSLLNVVCASN-FDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGN 186
            +K C   LLNV  ++N FDN F+ G ++G L         ++P+ MGF    SL Q+GN
Sbjct: 129 NNKGC---LLNVPSSANPFDNAFEFGSESGFLNQI------HAPISMGF---GSLTQLGN 188

Query: 187 QEMSLNNSDFPPGRLLPVVD---NPPAFGAGFNPAAAFESFDGCGNALF-QNRSKVLRPL 246
           +++S +  DF   R L   +   N      GF      E F    N  F  NR+KVL+PL
Sbjct: 189 RDLS-SVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEGFGSPANGGFVGNRAKVLKPL 248

Query: 247 EVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEED 306
           EV    G QPTLFQKRAA R  S    K+ N E S M               R  + + D
Sbjct: 249 EVLASSGAQPTLFQKRAAMRQSS--GSKMGNSESSGM---------------RRFSDDGD 308

Query: 307 IEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNL 366
           +++  ++VS  NY+SDE NE              S   A S   GG  KGKKKG+PAKNL
Sbjct: 309 MDETGIEVSGLNYESDEINE--------------SGKAAESVQIGGGGKGKKKGMPAKNL 368

Query: 367 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PG 426
           MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PG
Sbjct: 369 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPG 428

Query: 427 SLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSR 486
           SL   S+SFHPLTPTP TL CRVKEELCPSSL SPK Q ARVEVR+REGRAVNIHMFC R
Sbjct: 429 SLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGR 488

Query: 487 KPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAG 540
           +PGLLL+TM+ALDNLGLDVQQAVISCFNGFALDVFRAEQ +EGQEILP+QIKAVL D+AG
Sbjct: 489 RPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAG 494

BLAST of MS009864 vs. ExPASy Swiss-Prot
Match: Q9LPW3 (Transcription factor SCREAM2 OS=Arabidopsis thaliana OX=3702 GN=SCRM2 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.1e-105
Identity = 275/557 (49.37%), Postives = 324/557 (58.17%), Query Frame = 0

Query: 9   LWIDG-------DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 68
           +W+DG       ++ +AASW RN ++                    DW+ N+  P   ++
Sbjct: 6   VWLDGSGESPEVNNGEAASWVRNPDE--------------------DWF-NNPPPPQHTN 65

Query: 69  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL---NPIDSSSSCSP-SQAFALDPSHSQSQS 128
             DF            +  F  NPS+NLLL     IDSSSS SP    F LD +  Q Q 
Sbjct: 66  QNDFRF----------NGGFPLNPSENLLLLLQQSIDSSSSSSPLLHPFTLDAASQQQQQ 125

Query: 129 MLPPH-------KSCFSSLLNVVCASNFDNGF-DLGGDTGLLG-SYQGNPASNSPVLMGF 188
                       K+C  SLLNV   +N  N F D G D+G LG  + GN    SP  M F
Sbjct: 126 QQQQQEQSFLATKACIVSLLNVPTINN--NTFDDFGFDSGFLGQQFHGN--HQSPNSMNF 185

Query: 189 TGIS-SLPQIGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCG-NALFQ 248
           TG++ S+P            DF     LP  +N               S   CG + LF 
Sbjct: 186 TGLNHSVP------------DF-----LPAPEN---------------SSGSCGLSPLFS 245

Query: 249 NRSKVLRPLEVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEK 308
           NR+KVL+PL+V    G+QPTLFQKRAA R  S  + K+ N E S               +
Sbjct: 246 NRAKVLKPLQVMASSGSQPTLFQKRAAMRQSS--SSKMCNSESS--------------SE 305

Query: 309 LRNKNGEEDIED---GSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQ 368
           +R  + E +I+D   G +D+S  NY+SD+ N  N                          
Sbjct: 306 MRKSSYEREIDDTSTGIIDISGLNYESDDHNTNN-------------------------N 365

Query: 369 KGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN 428
           KGKKKG+PAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN
Sbjct: 366 KGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN 425

Query: 429 DLHNELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCP-SSLSSPKTQPARVEVRVRE 488
           DLH ELESTP S    S+S HPLTPTP TL  RVKEELCP SSL SPK Q  RVEVR+RE
Sbjct: 426 DLHTELESTPPS----SSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLRE 450

Query: 489 GRAVNIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILP 540
           G+AVNIHMFC R+PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQ +E  ++LP
Sbjct: 486 GKAVNIHMFCGRRPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQCQEDHDVLP 450

BLAST of MS009864 vs. ExPASy Swiss-Prot
Match: Q10S44 (Transcription factor BHLH3 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH3 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 1.3e-30
Identity = 82/198 (41.41%), Postives = 111/198 (56.06%), Query Frame = 0

Query: 340 GGDQKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELL 399
           G   + K  G P+KNLMAERRRRK+LNDRL MLRS+VPKISKMDR SILGD IDY+KEL 
Sbjct: 169 GVHPRSKLHGTPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDTIDYVKELT 228

Query: 400 QRINDLHNELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVR 459
           +RI  L  E+  TP  L                L   +K+    ++         + +V 
Sbjct: 229 ERIKTLEEEIGVTPEEL---------------DLLNTMKDSSSGNNNEMLVRNSTKFDVE 288

Query: 460 VREGRAVNIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQE 519
            R      I + C   PG+LLST+ AL+ LGL+++Q V+SCF+ F +     ++  + Q 
Sbjct: 289 NRGSGNTRIEICCPANPGVLLSTVSALEVLGLEIEQCVVSCFSDFGMQASCLQEDGKRQV 348

Query: 520 ILPEQIKAVLLDSAGLHG 538
           +  ++IK  L  SAG  G
Sbjct: 349 VSTDEIKQTLFRSAGYGG 351

BLAST of MS009864 vs. ExPASy Swiss-Prot
Match: Q9LSL1 (Transcription factor bHLH93 OS=Arabidopsis thaliana OX=3702 GN=BHLH93 PE=1 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.9e-29
Identity = 102/251 (40.64%), Postives = 137/251 (54.58%), Query Frame = 0

Query: 290 KNGEEDIEDGSVDVSRFNYDSDEPNEY--NKQEDNINTKNCGSNSNANSTVTGGDQKGKK 349
           +NG+E+          FN     P+    + Q  + +   CG  +N         +K KK
Sbjct: 129 QNGQEE---------NFNNPMSYPSPLMESDQSKSFSVGYCGGETN--------KKKSKK 188

Query: 350 -KGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLH 409
            +G P+KNLMAERRRRK+LNDRL MLRS+VPKISKMDR SILGDAIDY+KELL +IN L 
Sbjct: 189 LEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQ 248

Query: 410 NELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAV 469
           +E +      L  S + H              E   P   +SPK +  R +   R     
Sbjct: 249 DEEQE-----LGNSNNSHHSKLFGDLKDLNANE---PLVRNSPKFEIDRRDEDTR----- 308

Query: 470 NIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIK 529
            + + CS KPGLLLST+  L+ LGL+++Q VISCF+ F+L    +E +++   I  E IK
Sbjct: 309 -VDICCSPKPGLLLSTVNTLETLGLEIEQCVISCFSDFSLQASCSEGAEQRDFITSEDIK 348

Query: 530 AVLLDSAGLHG 538
             L  +AG  G
Sbjct: 369 QALFRNAGYGG 348

BLAST of MS009864 vs. ExPASy Swiss-Prot
Match: Q336V8 (Basic helix-loop-helix protein 004 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH004 PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.6e-28
Identity = 90/213 (42.25%), Postives = 119/213 (55.87%), Query Frame = 0

Query: 329 GSNSNANSTVTGGDQKGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASIL 388
           G    A ++V     +  + G P+KNLMAERRRRK+LNDRL MLRSVVP+ISKMDR SIL
Sbjct: 172 GGGGGAAASVRPRSCRPPQPGAPSKNLMAERRRRKRLNDRLSMLRSVVPRISKMDRTSIL 231

Query: 389 GDAIDYLKELLQRINDLHNELES--TPGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSL 448
           GD I Y+KEL+ RI +L  E  +  +  S  +  +     T  PP           PSS 
Sbjct: 232 GDTIGYVKELMDRIKNLQVEAATGDSSSSSTENLSMLKLNTLKPP-----------PSSS 291

Query: 449 SSPKT----QPARVEVRVREGRAVNIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFN 508
           S  +T       R EV  RE  +  I M C+  P LL ST+ AL+ LG++++Q VISCF+
Sbjct: 292 SGEETPLIRNSTRFEVERRENGSTRIEMACAAIPELLPSTLAALEALGVEIEQCVISCFD 351

Query: 509 GFALDVFRAEQSKEGQEIL-PEQIKAVLLDSAG 535
            FA+     +  K+ +     E+IK  L  SAG
Sbjct: 352 DFAMQASCLQDDKKREMTRDTEEIKQTLFRSAG 373

BLAST of MS009864 vs. ExPASy TrEMBL
Match: A0A0A0LBB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598900 PE=4 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 3.8e-243
Identity = 454/544 (83.46%), Postives = 479/544 (88.05%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASW-TRNNNDNDDDIAI--AGSISSFKSMLEPDWYINSSTPL 60
           MLPK TGILW+DGDH+DA SW T  N+DN   + +  A SISSFKS+L+ D YINSSTPL
Sbjct: 1   MLPKSTGILWLDGDHEDATSWITTTNHDNHHSLPVPPAPSISSFKSILQSDCYINSSTPL 60

Query: 61  NPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQS 120
           NPS  QDFHSLS  SH HIPDL FSSNPSDN LL+P+DSSSSCSPS AF L PSH  S S
Sbjct: 61  NPSH-QDFHSLS--SHPHIPDLPFSSNPSDNFLLHPLDSSSSCSPSHAFPLHPSH--SNS 120

Query: 121 MLPPHK-SCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQ 180
           +LP HK SCFSSLLNVVC S+FDN FDLGG+ GLLGSYQGN A     LMGFTGISSLPQ
Sbjct: 121 ILPSHKSSCFSSLLNVVCGSSFDNAFDLGGENGLLGSYQGNQA-----LMGFTGISSLPQ 180

Query: 181 IGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLE 240
           I  QE++LNNSDF       V DNPPAFGAGFN AAAFE+FD CGNALFQNRSKVLRPLE
Sbjct: 181 IATQELTLNNSDF------HVPDNPPAFGAGFNSAAAFENFDACGNALFQNRSKVLRPLE 240

Query: 241 VFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDI 300
           VFP+VG QPTLFQKRAAHRVGS GADKLENLEVS  KL EG MWT NLE+LR  NG+EDI
Sbjct: 241 VFPEVGAQPTLFQKRAAHRVGSGGADKLENLEVSGFKLEEGPMWTPNLERLRKMNGDEDI 300

Query: 301 EDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLM 360
           EDGSVDVSRFNYDSDEP EYNKQEDN+N KNCGSNSNANST+TGGDQKGKKKGLPAKNLM
Sbjct: 301 EDGSVDVSRFNYDSDEPIEYNKQEDNVNAKNCGSNSNANSTITGGDQKGKKKGLPAKNLM 360

Query: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PGS 420
           AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLH ELEST PGS
Sbjct: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHKELESTPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
           +LQPSASFHPLTPT PTLPCRVKEELCPS+LSSP TQPARVEVRVREGRAVNIHMFCSRK
Sbjct: 421 VLQPSASFHPLTPTLPTLPCRVKEELCPSNLSSPNTQPARVEVRVREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTMRALDNLGLD+QQAVISCFNGFALDVFRAEQSKEGQE+LPEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMRALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGQEMLPEQIKAVLLDSAGL 528

BLAST of MS009864 vs. ExPASy TrEMBL
Match: A0A1S3BMQ8 (transcription factor ICE1 OS=Cucumis melo OX=3656 GN=LOC103491734 PE=4 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.3e-240
Identity = 451/544 (82.90%), Postives = 476/544 (87.50%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASW-TRNNNDNDDDIAI--AGSISSFKSMLEPDWYINSSTPL 60
           MLPK TGILW+DGDH+DA SW T  N+DN   + I  A SISSFKS+L  D YINSSTPL
Sbjct: 1   MLPKSTGILWLDGDHEDATSWTTTTNHDNHHSLPIPPAPSISSFKSILPSDCYINSSTPL 60

Query: 61  NPSSLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQS 120
           NPS  QDF SLS  SH H+PDL FSSNPSD+ LL+P+DSSSSCSPS AF L PSH  S S
Sbjct: 61  NPSH-QDFPSLS--SHPHLPDLPFSSNPSDDFLLHPLDSSSSCSPSHAFPLHPSH--SNS 120

Query: 121 MLPPHK-SCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQ 180
           +LP HK SCFSSLLNVVC S+FDN FDLGG+ GLLGSYQGN A     LMGFTGISSLPQ
Sbjct: 121 ILPSHKSSCFSSLLNVVCGSSFDNAFDLGGENGLLGSYQGNQA-----LMGFTGISSLPQ 180

Query: 181 IGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLE 240
           I NQE++LNNSDF      PV DN PAFG GFN AAAFE+FD CGNALFQNRSKVLRPLE
Sbjct: 181 IANQELTLNNSDF------PVPDNSPAFGPGFNSAAAFENFDACGNALFQNRSKVLRPLE 240

Query: 241 VFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDI 300
           VFP+VG QPTLFQKRAAHRVGS G DKLENLEVS  KLGEGSMWT NLE+LR  NG+EDI
Sbjct: 241 VFPEVGVQPTLFQKRAAHRVGSGGPDKLENLEVSGFKLGEGSMWTPNLERLRKMNGDEDI 300

Query: 301 EDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLM 360
           EDGSVDVSR NYDSDEP EYNKQEDN+N K CGS+SNANST+TGGDQKGKKKGLPAKNLM
Sbjct: 301 EDGSVDVSRLNYDSDEPIEYNKQEDNVNAKKCGSHSNANSTITGGDQKGKKKGLPAKNLM 360

Query: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PGS 420
           AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PGS
Sbjct: 361 AERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
           +LQPSASFHPLTPT PTLPCRVKEELCPS+LSSP  QPARVEVRVREGRAVNIHMFCSRK
Sbjct: 421 VLQPSASFHPLTPTLPTLPCRVKEELCPSNLSSPYAQPARVEVRVREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTMRALDNLGLD+QQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMRALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 528

BLAST of MS009864 vs. ExPASy TrEMBL
Match: A0A6J1J7U5 (transcription factor ICE1-like OS=Cucurbita maxima OX=3661 GN=LOC111482145 PE=4 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 9.7e-239
Identity = 434/544 (79.78%), Postives = 473/544 (86.95%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPS 60
           MLPK TGILW+DGDH D ASW R NN N+DDIA+AGS SSFKSML+ DWY+NS+TPLNPS
Sbjct: 1   MLPKSTGILWLDGDHHDGASWNRTNNHNNDDIAVAGSASSFKSMLQSDWYMNSTTPLNPS 60

Query: 61  SLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLP 120
           + QDFHSLS  SH  + +L F+SNP DNLLL PIDSSS+CS SQAF+LDPSH  SQSMLP
Sbjct: 61  N-QDFHSLS--SHPDVRNLPFNSNPPDNLLLRPIDSSSACSASQAFSLDPSH--SQSMLP 120

Query: 121 PHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQ 180
           P KSCF SL NV C+SNF+NGFDLGG+ GLLGSYQGN  SNS  +MGF GISSLPQIGNQ
Sbjct: 121 PQKSCFPSLFNVACSSNFENGFDLGGENGLLGSYQGNQVSNSSAMMGFNGISSLPQIGNQ 180

Query: 181 EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQ 240
           E+SLNN++F      PV DNPPAFGAGFNP  AFESFDGCGNALFQNRSK LRP E FPQ
Sbjct: 181 ELSLNNAEF------PVADNPPAFGAGFNPTDAFESFDGCGNALFQNRSKALRPPESFPQ 240

Query: 241 V-GTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDG 300
           V GTQPTLFQKRAAHRVGS+GADKLENLEVS  K G+GSMW  N+E+L  KN +EDIED 
Sbjct: 241 VGGTQPTLFQKRAAHRVGSSGADKLENLEVSGSKFGDGSMWMPNMERLM-KNSDEDIEDD 300

Query: 301 SVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAER 360
           S+D+S  NYDSDE NEYNK EDN+N KN GSNSNAN+TV GG+QKGK+KG+PAKNLMAER
Sbjct: 301 SLDISHLNYDSDEANEYNKLEDNVNAKNSGSNSNANTTVAGGNQKGKRKGMPAKNLMAER 360

Query: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST----PGS 420
           RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQ+IN+LHNELEST    PGS
Sbjct: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQKINELHNELESTPPPPPGS 420

Query: 421 LLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSRK 480
            LQP ASFHP TPT P LPCRVK+ELCPSS+SSPK QPARVEVR REGRAVNIHMFCSRK
Sbjct: 421 SLQPPASFHPSTPTLPNLPCRVKDELCPSSMSSPKAQPARVEVRAREGRAVNIHMFCSRK 480

Query: 481 PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAGL 540
           PGLLLSTM+ALDNLGLDVQQAV+SCFNGFALDVFRAEQSKEG+EI PEQIKAVLLDSAGL
Sbjct: 481 PGLLLSTMKALDNLGLDVQQAVVSCFNGFALDVFRAEQSKEGEEISPEQIKAVLLDSAGL 532

BLAST of MS009864 vs. ExPASy TrEMBL
Match: A0A6J1F1N2 (transcription factor ICE1-like OS=Cucurbita moschata OX=3662 GN=LOC111441385 PE=4 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 6.3e-238
Identity = 437/551 (79.31%), Postives = 474/551 (86.03%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPS 60
           MLPK TGILW+DGDH D ASW R NN N+DDIA+AGS SSFKSML+ DWY+NS+TPLNPS
Sbjct: 1   MLPKSTGILWLDGDHHDGASWNRTNNHNNDDIAVAGSASSFKSMLQSDWYMNSTTPLNPS 60

Query: 61  SLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLP 120
           + QDFHSLS  SH  + DL F+SNP DNLLL PIDSSS+CS SQAFALDPSH  SQSMLP
Sbjct: 61  N-QDFHSLS--SHPDVRDLPFNSNPPDNLLLRPIDSSSACSASQAFALDPSH--SQSMLP 120

Query: 121 PHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQ 180
           P KSCF SL NV C+SNF+NGFDLGG+ GLLGSYQGN  SNS  +MGF GISSLPQIGNQ
Sbjct: 121 PQKSCFPSLYNVACSSNFENGFDLGGENGLLGSYQGNQVSNSSGMMGFNGISSLPQIGNQ 180

Query: 181 EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQ 240
           E+SLNN++F      PV DNPPAFGAGFNP  AFESFDGCGNALFQNRSK LRP E FPQ
Sbjct: 181 ELSLNNAEF------PVADNPPAFGAGFNPTDAFESFDGCGNALFQNRSKALRPPESFPQ 240

Query: 241 V-GTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDG 300
           V GTQPTLFQKRAAHRVGS+G DKLENLEVS  K G+GSMW  N+E+L  KN +EDIED 
Sbjct: 241 VGGTQPTLFQKRAAHRVGSSGVDKLENLEVSGSKFGDGSMWMPNMERLM-KNSDEDIEDD 300

Query: 301 SVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAER 360
           S+D+SR NYDSDE NEYNK EDN+N KN GSNSNAN+TVT G+QKGK+KG+PAKNLMAER
Sbjct: 301 SLDISRLNYDSDEANEYNKLEDNVNAKNSGSNSNANTTVTDGNQKGKRKGMPAKNLMAER 360

Query: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST------P 420
           RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQ+IN+LHNELEST      P
Sbjct: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQKINELHNELESTPPPPPPP 420

Query: 421 GSLLQPSASFHPLTPT-----PPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNI 480
           GS LQP ASFHPLTPT      P LPCRVKEELCPSS+SSPK QPARVEVR REGRAVNI
Sbjct: 421 GSSLQPPASFHPLTPTLPIPMLPNLPCRVKEELCPSSMSSPKAQPARVEVRAREGRAVNI 480

Query: 481 HMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAV 540
           HMFCSRKPGLLLSTM+ALDNLGLD+QQAVISCFNGFALDVFRAEQSKEG+E+ PEQIKAV
Sbjct: 481 HMFCSRKPGLLLSTMKALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGEEVSPEQIKAV 539

BLAST of MS009864 vs. ExPASy TrEMBL
Match: A0A4Y5WS37 (ICE1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 3.1e-237
Identity = 436/547 (79.71%), Postives = 472/547 (86.29%), Query Frame = 0

Query: 1   MLPKPTGILWIDGDHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPS 60
           MLPK TGILW+DGDH D ASW R NN N+DDIA+AGS SSFKSML+ DWY+NS+TPLNPS
Sbjct: 1   MLPKSTGILWLDGDHHDGASWNRTNNHNNDDIAVAGSASSFKSMLQSDWYMNSTTPLNPS 60

Query: 61  SLQDFHSLSSASHHHIPDLAFSSNPSDNLLLNPIDSSSSCSPSQAFALDPSHSQSQSMLP 120
           + QDFHSLS  SH  + DL F+SNP DNLLL PIDSSS+CS SQAFA+DPSH  SQSMLP
Sbjct: 61  N-QDFHSLS--SHPDVRDLPFNSNPPDNLLLRPIDSSSACSASQAFAMDPSH--SQSMLP 120

Query: 121 PHKSCFSSLLNVVCASNFDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGNQ 180
           P KSCF SL NV C+SNF+NGFDLGG+ GLLGSYQGN  SNS  +MGF GISSLPQIGNQ
Sbjct: 121 PQKSCFPSLFNVACSSNFENGFDLGGENGLLGSYQGNQVSNSSGMMGFNGISSLPQIGNQ 180

Query: 181 EMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCGNALFQNRSKVLRPLEVFPQ 240
           E+SLNN++F      PV DNPPAFGAGFNP  AFESFDGCGNALFQNRSK LRP E FPQ
Sbjct: 181 ELSLNNAEF------PVADNPPAFGAGFNPTDAFESFDGCGNALFQNRSKALRPPESFPQ 240

Query: 241 V-GTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEEDIEDG 300
           V GTQPTLFQKRAAHRVGS+G DKLENLEVS  K G+GSMW  N+E+L  KN +EDIED 
Sbjct: 241 VGGTQPTLFQKRAAHRVGSSGVDKLENLEVSGSKFGDGSMWMPNMERLM-KNSDEDIEDD 300

Query: 301 SVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNLMAER 360
           S+D+SR NYDSDE NEYNK EDN+N KN GSNSNAN+TVT G+QKGK+KG+PAKNLMAER
Sbjct: 301 SLDISRLNYDSDEANEYNKLEDNVNAKNSGSNSNANTTVTDGNQKGKRKGMPAKNLMAER 360

Query: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST---PGSL 420
           RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQ+IN+LHNELEST   PGS 
Sbjct: 361 RRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQKINELHNELESTPPPPGSS 420

Query: 421 LQPSASFHPLT-----PTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMF 480
           LQP AS HPLT     PT P LPCRVKEELCPSS+SSPK QPARVEVR REGRAVNIHMF
Sbjct: 421 LQPPASIHPLTPTLPIPTLPNLPCRVKEELCPSSMSSPKAQPARVEVRAREGRAVNIHMF 480

Query: 481 CSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLD 539
           CSRKPGLLLSTM+ALDNLGLD+QQAVISCFNGFALDVFRAEQSKEG+EI PEQIKAVLLD
Sbjct: 481 CSRKPGLLLSTMKALDNLGLDIQQAVISCFNGFALDVFRAEQSKEGEEISPEQIKAVLLD 535

BLAST of MS009864 vs. TAIR 10
Match: AT3G26744.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.2e-129
Identity = 297/545 (54.50%), Postives = 346/545 (63.49%), Query Frame = 0

Query: 7   GILWIDG-----DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 66
           G +W++G     + ++  SW RN  D           S FK MLE DW+  SS   +P  
Sbjct: 9   GGVWLNGGGGEREENEEGSWGRNQEDGS---------SQFKPMLEGDWF--SSNQPHPQD 68

Query: 67  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL-NPIDSSSSCSPSQAFALDPSHSQSQSMLP 126
           LQ   +     +       F  NP+DNLLL + IDSSSSCSPSQAF+LDPS         
Sbjct: 69  LQMLQNQPDFRYFG----GFPFNPNDNLLLQHSIDSSSSCSPSQAFSLDPSQQNQFLSTN 128

Query: 127 PHKSCFSSLLNVVCASN-FDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGN 186
            +K C   LLNV  ++N FDN F+ G ++G L         ++P+ MGF    SL Q+GN
Sbjct: 129 NNKGC---LLNVPSSANPFDNAFEFGSESGFLNQI------HAPISMGF---GSLTQLGN 188

Query: 187 QEMSLNNSDFPPGRLLPVVD---NPPAFGAGFNPAAAFESFDGCGNALF-QNRSKVLRPL 246
           +++S +  DF   R L   +   N      GF      E F    N  F  NR+KVL+PL
Sbjct: 189 RDLS-SVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEGFGSPANGGFVGNRAKVLKPL 248

Query: 247 EVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEED 306
           EV    G QPTLFQKRAA R  S    K+ N E S M               R  + + D
Sbjct: 249 EVLASSGAQPTLFQKRAAMRQSS--GSKMGNSESSGM---------------RRFSDDGD 308

Query: 307 IEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNL 366
           +++  ++VS  NY+SDE NE              S   A S   GG  KGKKKG+PAKNL
Sbjct: 309 MDETGIEVSGLNYESDEINE--------------SGKAAESVQIGGGGKGKKKGMPAKNL 368

Query: 367 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PG 426
           MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PG
Sbjct: 369 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPG 428

Query: 427 SLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSR 486
           SL   S+SFHPLTPTP TL CRVKEELCPSSL SPK Q ARVEVR+REGRAVNIHMFC R
Sbjct: 429 SLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGR 488

Query: 487 KPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAG 540
           +PGLLL+TM+ALDNLGLDVQQAVISCFNGFALDVFRAEQ +EGQEILP+QIKAVL D+AG
Sbjct: 489 RPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAG 494

BLAST of MS009864 vs. TAIR 10
Match: AT3G26744.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.2e-129
Identity = 297/545 (54.50%), Postives = 346/545 (63.49%), Query Frame = 0

Query: 7   GILWIDG-----DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 66
           G +W++G     + ++  SW RN  D           S FK MLE DW+  SS   +P  
Sbjct: 9   GGVWLNGGGGEREENEEGSWGRNQEDGS---------SQFKPMLEGDWF--SSNQPHPQD 68

Query: 67  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL-NPIDSSSSCSPSQAFALDPSHSQSQSMLP 126
           LQ   +     +       F  NP+DNLLL + IDSSSSCSPSQAF+LDPS         
Sbjct: 69  LQMLQNQPDFRYFG----GFPFNPNDNLLLQHSIDSSSSCSPSQAFSLDPSQQNQFLSTN 128

Query: 127 PHKSCFSSLLNVVCASN-FDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGN 186
            +K C   LLNV  ++N FDN F+ G ++G L         ++P+ MGF    SL Q+GN
Sbjct: 129 NNKGC---LLNVPSSANPFDNAFEFGSESGFLNQI------HAPISMGF---GSLTQLGN 188

Query: 187 QEMSLNNSDFPPGRLLPVVD---NPPAFGAGFNPAAAFESFDGCGNALF-QNRSKVLRPL 246
           +++S +  DF   R L   +   N      GF      E F    N  F  NR+KVL+PL
Sbjct: 189 RDLS-SVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEGFGSPANGGFVGNRAKVLKPL 248

Query: 247 EVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEED 306
           EV    G QPTLFQKRAA R  S    K+ N E S M               R  + + D
Sbjct: 249 EVLASSGAQPTLFQKRAAMRQSS--GSKMGNSESSGM---------------RRFSDDGD 308

Query: 307 IEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNL 366
           +++  ++VS  NY+SDE NE              S   A S   GG  KGKKKG+PAKNL
Sbjct: 309 MDETGIEVSGLNYESDEINE--------------SGKAAESVQIGGGGKGKKKGMPAKNL 368

Query: 367 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PG 426
           MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PG
Sbjct: 369 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPG 428

Query: 427 SLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSR 486
           SL   S+SFHPLTPTP TL CRVKEELCPSSL SPK Q ARVEVR+REGRAVNIHMFC R
Sbjct: 429 SLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGR 488

Query: 487 KPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAG 540
           +PGLLL+TM+ALDNLGLDVQQAVISCFNGFALDVFRAEQ +EGQEILP+QIKAVL D+AG
Sbjct: 489 RPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAG 494

BLAST of MS009864 vs. TAIR 10
Match: AT3G26744.4 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 458.8 bits (1179), Expect = 6.2e-129
Identity = 297/545 (54.50%), Postives = 346/545 (63.49%), Query Frame = 0

Query: 7   GILWIDG-----DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 66
           G +W++G     + ++  SW RN  D           S FK MLE DW+  SS   +P  
Sbjct: 9   GGVWLNGGGGEREENEEGSWGRNQEDGS---------SQFKPMLEGDWF--SSNQPHPQD 68

Query: 67  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL-NPIDSSSSCSPSQAFALDPSHSQSQSMLP 126
           LQ   +     +       F  NP+DNLLL + IDSSSSCSPSQAF+LDPS         
Sbjct: 69  LQMLQNQPDFRYFG----GFPFNPNDNLLLQHSIDSSSSCSPSQAFSLDPSQQNQFLSTN 128

Query: 127 PHKSCFSSLLNVVCASN-FDNGFDLGGDTGLLGSYQGNPASNSPVLMGFTGISSLPQIGN 186
            +K C   LLNV  ++N FDN F+ G ++G L         ++P+ MGF    SL Q+GN
Sbjct: 129 NNKGC---LLNVPSSANPFDNAFEFGSESGFLNQI------HAPISMGF---GSLTQLGN 188

Query: 187 QEMSLNNSDFPPGRLLPVVD---NPPAFGAGFNPAAAFESFDGCGNALF-QNRSKVLRPL 246
           +++S +  DF   R L   +   N      GF      E F    N  F  NR+KVL+PL
Sbjct: 189 RDLS-SVPDFLSARSLLAPESNNNNTMLCGGFTAPLELEGFGSPANGGFVGNRAKVLKPL 248

Query: 247 EVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEKLRNKNGEED 306
           EV    G QPTLFQKRAA R  S    K+ N E S M               R  + + D
Sbjct: 249 EVLASSGAQPTLFQKRAAMRQSS--GSKMGNSESSGM---------------RRFSDDGD 308

Query: 307 IEDGSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQKGKKKGLPAKNL 366
           +++  ++VS  NY+SDE NE              S   A S   GG  KGKKKG+PAKNL
Sbjct: 309 MDETGIEVSGLNYESDEINE--------------SGKAAESVQIGGGGKGKKKGMPAKNL 368

Query: 367 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST-PG 426
           MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELEST PG
Sbjct: 369 MAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLHNELESTPPG 428

Query: 427 SLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAVNIHMFCSR 486
           SL   S+SFHPLTPTP TL CRVKEELCPSSL SPK Q ARVEVR+REGRAVNIHMFC R
Sbjct: 429 SLPPTSSSFHPLTPTPQTLSCRVKEELCPSSLPSPKGQQARVEVRLREGRAVNIHMFCGR 488

Query: 487 KPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIKAVLLDSAG 540
           +PGLLL+TM+ALDNLGLDVQQAVISCFNGFALDVFRAEQ +EGQEILP+QIKAVL D+AG
Sbjct: 489 RPGLLLATMKALDNLGLDVQQAVISCFNGFALDVFRAEQCQEGQEILPDQIKAVLFDTAG 494

BLAST of MS009864 vs. TAIR 10
Match: AT1G12860.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 384.4 bits (986), Expect = 1.5e-106
Identity = 275/557 (49.37%), Postives = 324/557 (58.17%), Query Frame = 0

Query: 9   LWIDG-------DHDDAASWTRNNNDNDDDIAIAGSISSFKSMLEPDWYINSSTPLNPSS 68
           +W+DG       ++ +AASW RN ++                    DW+ N+  P   ++
Sbjct: 6   VWLDGSGESPEVNNGEAASWVRNPDE--------------------DWF-NNPPPPQHTN 65

Query: 69  LQDFHSLSSASHHHIPDLAFSSNPSDNLLL---NPIDSSSSCSP-SQAFALDPSHSQSQS 128
             DF            +  F  NPS+NLLL     IDSSSS SP    F LD +  Q Q 
Sbjct: 66  QNDFRF----------NGGFPLNPSENLLLLLQQSIDSSSSSSPLLHPFTLDAASQQQQQ 125

Query: 129 MLPPH-------KSCFSSLLNVVCASNFDNGF-DLGGDTGLLG-SYQGNPASNSPVLMGF 188
                       K+C  SLLNV   +N  N F D G D+G LG  + GN    SP  M F
Sbjct: 126 QQQQQEQSFLATKACIVSLLNVPTINN--NTFDDFGFDSGFLGQQFHGN--HQSPNSMNF 185

Query: 189 TGIS-SLPQIGNQEMSLNNSDFPPGRLLPVVDNPPAFGAGFNPAAAFESFDGCG-NALFQ 248
           TG++ S+P            DF     LP  +N               S   CG + LF 
Sbjct: 186 TGLNHSVP------------DF-----LPAPEN---------------SSGSCGLSPLFS 245

Query: 249 NRSKVLRPLEVFPQVGTQPTLFQKRAAHRVGSAGADKLENLEVSDMKLGEGSMWTANLEK 308
           NR+KVL+PL+V    G+QPTLFQKRAA R  S  + K+ N E S               +
Sbjct: 246 NRAKVLKPLQVMASSGSQPTLFQKRAAMRQSS--SSKMCNSESS--------------SE 305

Query: 309 LRNKNGEEDIED---GSVDVSRFNYDSDEPNEYNKQEDNINTKNCGSNSNANSTVTGGDQ 368
           +R  + E +I+D   G +D+S  NY+SD+ N  N                          
Sbjct: 306 MRKSSYEREIDDTSTGIIDISGLNYESDDHNTNN-------------------------N 365

Query: 369 KGKKKGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN 428
           KGKKKG+PAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN
Sbjct: 366 KGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRIN 425

Query: 429 DLHNELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCP-SSLSSPKTQPARVEVRVRE 488
           DLH ELESTP S    S+S HPLTPTP TL  RVKEELCP SSL SPK Q  RVEVR+RE
Sbjct: 426 DLHTELESTPPS----SSSLHPLTPTPQTLSYRVKEELCPSSSLPSPKGQQPRVEVRLRE 450

Query: 489 GRAVNIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILP 540
           G+AVNIHMFC R+PGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQ +E  ++LP
Sbjct: 486 GKAVNIHMFCGRRPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQCQEDHDVLP 450

BLAST of MS009864 vs. TAIR 10
Match: AT5G65640.1 (beta HLH protein 93 )

HSP 1 Score: 132.1 bits (331), Expect = 1.3e-30
Identity = 102/251 (40.64%), Postives = 137/251 (54.58%), Query Frame = 0

Query: 290 KNGEEDIEDGSVDVSRFNYDSDEPNEY--NKQEDNINTKNCGSNSNANSTVTGGDQKGKK 349
           +NG+E+          FN     P+    + Q  + +   CG  +N         +K KK
Sbjct: 129 QNGQEE---------NFNNPMSYPSPLMESDQSKSFSVGYCGGETN--------KKKSKK 188

Query: 350 -KGLPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIDYLKELLQRINDLH 409
            +G P+KNLMAERRRRK+LNDRL MLRS+VPKISKMDR SILGDAIDY+KELL +IN L 
Sbjct: 189 LEGQPSKNLMAERRRRKRLNDRLSMLRSIVPKISKMDRTSILGDAIDYMKELLDKINKLQ 248

Query: 410 NELESTPGSLLQPSASFHPLTPTPPTLPCRVKEELCPSSLSSPKTQPARVEVRVREGRAV 469
           +E +      L  S + H              E   P   +SPK +  R +   R     
Sbjct: 249 DEEQE-----LGNSNNSHHSKLFGDLKDLNANE---PLVRNSPKFEIDRRDEDTR----- 308

Query: 470 NIHMFCSRKPGLLLSTMRALDNLGLDVQQAVISCFNGFALDVFRAEQSKEGQEILPEQIK 529
            + + CS KPGLLLST+  L+ LGL+++Q VISCF+ F+L    +E +++   I  E IK
Sbjct: 309 -VDICCSPKPGLLLSTVNTLETLGLEIEQCVISCFSDFSLQASCSEGAEQRDFITSEDIK 348

Query: 530 AVLLDSAGLHG 538
             L  +AG  G
Sbjct: 369 QALFRNAGYGG 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893918.17.6e-25486.05transcription factor ICE1 [Benincasa hispida][more]
XP_011651587.17.9e-24383.46transcription factor ICE1 [Cucumis sativus] >KGN58244.1 hypothetical protein Csa... [more]
XP_008450018.14.8e-24082.90PREDICTED: transcription factor ICE1 [Cucumis melo][more]
XP_022983584.12.0e-23879.78transcription factor ICE1-like [Cucurbita maxima][more]
XP_022934114.11.3e-23779.31transcription factor ICE1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9LSE28.7e-12854.50Transcription factor ICE1 OS=Arabidopsis thaliana OX=3702 GN=SCRM PE=1 SV=1[more]
Q9LPW32.1e-10549.37Transcription factor SCREAM2 OS=Arabidopsis thaliana OX=3702 GN=SCRM2 PE=1 SV=1[more]
Q10S441.3e-3041.41Transcription factor BHLH3 OS=Oryza sativa subsp. japonica OX=39947 GN=BHLH3 PE=... [more]
Q9LSL11.9e-2940.64Transcription factor bHLH93 OS=Arabidopsis thaliana OX=3702 GN=BHLH93 PE=1 SV=1[more]
Q336V84.6e-2842.25Basic helix-loop-helix protein 004 OS=Oryza sativa subsp. japonica OX=39947 GN=B... [more]
Match NameE-valueIdentityDescription
A0A0A0LBB43.8e-24383.46Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G598900 PE=4 SV=1[more]
A0A1S3BMQ82.3e-24082.90transcription factor ICE1 OS=Cucumis melo OX=3656 GN=LOC103491734 PE=4 SV=1[more]
A0A6J1J7U59.7e-23979.78transcription factor ICE1-like OS=Cucurbita maxima OX=3661 GN=LOC111482145 PE=4 ... [more]
A0A6J1F1N26.3e-23879.31transcription factor ICE1-like OS=Cucurbita moschata OX=3662 GN=LOC111441385 PE=... [more]
A0A4Y5WS373.1e-23779.71ICE1 OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT3G26744.16.2e-12954.50basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G26744.26.2e-12954.50basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G26744.46.2e-12954.50basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT1G12860.11.5e-10649.37basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT5G65640.11.3e-3040.64beta HLH protein 93 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 392..412
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 311..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 320..342
NoneNo IPR availablePANTHERPTHR31945:SF98BHLH TRANSCRIPTIONAL FACTORcoord: 100..538
NoneNo IPR availablePANTHERPTHR31945TRANSCRIPTION FACTOR SCREAM2-RELATEDcoord: 100..538
NoneNo IPR availableCDDcd11443bHLH_AtAMS_likecoord: 352..415
e-value: 2.03971E-40
score: 138.665
NoneNo IPR availableCDDcd04873ACT_UUR-ACR-likecoord: 468..528
e-value: 2.44737E-7
score: 46.0021
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 355..404
e-value: 4.2E-14
score: 62.9
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 356..398
e-value: 2.2E-9
score: 37.2
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 349..398
score: 15.681567
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3D4.10.280.10coord: 343..413
e-value: 2.0E-15
score: 58.4
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 352..423
IPR002912ACT domainPROSITEPS51671ACTcoord: 467..539
score: 9.085968

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009864.1MS009864.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0046983 protein dimerization activity