MS009793 (gene) Bitter gourd (TR) v1

Overview
NameMS009793
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionCytosolic Fe-S cluster assembly factor NBP35
Locationscaffold173: 332243 .. 335049 (+)
RNA-Seq ExpressionMS009793
SyntenyMS009793
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GATTGCCCTGGTCCTCAGTCGGAGTCCGCGGGGAAATCCGACGCCTGCCAAGGATGTCCCAACCAAGAAATTTGTGCTACTGCTCCCAAAGGCCCTGACCCAGGTCCCCTCTCTCCATAAACCCTATGAATCTGTTTCTGTTTTACTTTCTGTTGCTTGCAATTGAGATTTGGATGAGTTTCTGCTTACATCTTCTGAGCTGTTGGGGCTGCTTGGTTTTCTGTTTGGCTGGTTTTTGAGGCCAACAACGATATCACTTTATGAGAATGGGGCACCTCTTATTGGTATTTGTTTGCTTCAATAATTGGGCACTAGTCTTGGGGACTGAGGAGGCTCAATCCACTCTGGTTTCAACTTGAAAAGGCAACTAGAGTTTTAAGTTATGAAATTTTGGTTTGAAATTTGAATTCTATTTTGATGTTTGAACTTTCAAGATTATTCCATTTTGGTTCCTAGACTTTCAGATTTATTCCATTTTGGTCCAGGAAATTTCATAATGTGTATTTTAATTCTGGACTTAAAAACAATCATTCTGGTATTTGCTTTGTATTTTAAAGTTGATAATGTGGCTTCAACTATGTGTATAATGTGTTAACATGAGCATGTATTCAAACTATATAGGTTGGATGCAAGTATTAGGTGAGCGGATATGAGGTGATGAAATAGTCATTGTTTTGAAAATTTAGAAGGTTTAGGCACCGAATCGAGAATGATTATTTTTTTTTAAAGTTTAGAGACTAAAATAGATTGTTTTAACATTTAGGGACCAAAATGAAATAAACCAATTTTAGGGACTTTAATAGGATTTAATTTTAAACTTCAAATTTTTGTGAAAGTAGAGGATATCTCTAAACATCCCAACCCCCTACACCACACCAATATCTCAAACCAATATGTCTCTTATTTGTATTGCGATTCTCTCAAACCAAACAGAACATCTAAGTTTATGACCTTTTACAAGCTTTGAGTTTGTTTTTAGTTTATATAGCGCAGTGGTGGGTGTTGTGAACTGTGAAGCATTTACTGTCCTAGTAGGTAATTGTGATGGTTTTTCCGTTTTATTCTTTATTTTTCCATCTGCATTTTTATTTGGTATCAGGGTTTGTTTTATGTTTCGGTTTTATTTTTCATCCATCTCTAAAAATTAGCAAGAGAAAGGCAAAGAAGAAGAAAGTTAATTGCCATTTTTTCCCTTCCTCTAGTTGCATTTTAACATGTTATCTAGAACCGGATCATAGTTACTTGGATAGAAAGAAATAGGAAAATTTTCAACTGTGTTGGGCATCCATAAGTGCCTCTGTTTGGTTCGTCGGGTCAAATTTTTGTGATGACATTCCTTCATTCTTGACTTGGAGCCCTTTCTTATTATTATCTTGGCTCCCTTTTTGCCTCTTGGGCCTGTGTTTATCTATAGCCTTTCTTGTATGTAATCTTTCATTCTCCCTCCTGTCCTGACTGATTTTTTGTCTGAAAAAGAAATTTGGAATTGATGAACGGGTCATTTTGAATATGATTTATAGTTTCTGTTCCTGATAGTAGGTCAAATCTGTCTCCTTTGCGAGTTCAGCAACTTTTTTCTTGCATAGCACTGAGTGTTTAAACCTGTTTGGTTTTCTGAATCCTATGAGAATTCATTAGTAGAATCTATTCTCTCTTTCAAGAATTTTTTTTTGCTCCTACAGGCTTCTTGCTCATGATTATTAACGATTGGAAGACTCTCCTTTTGTCCTTTTGTCATTCTGTGGGCAGGGGCTTTCTCTACCCGAGCCCCAAGGCCGTTTTTCCTTCTTTTTTCTTTTGCTCTCTAACATATTACCTGTTTGTGTGTTTATCCTTATTTACCGTTCCCGACACACCTCTAATGCTGCGTATTTGTCTTTTCAGACCTGGTTGCGATAGCAGAACGAATGGCAACTGTTAAGCACAAGATTCTGGTTCTATCAGGGAAGGGTGGAGTCGGAAAGAGCACGTTCTCTGCTCAACTATCGTTTGCACTAGCAGCCATGGACTTCCAGGTAGGCCTCCTAGACATCGATATTTGTGGCCCAAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGCAACCTTGGATGGTCCCCTGTTTACGTAGAGTCCAACCTTGGAGTCATGTCAATAGGGTTCATGCTCCCAAACCCAGACGAAGCTGTCATTTGGAGAGGCCCTCGAAAAAACGGTCTCATCAAGCAGTTTCTAAAGGACGTGTACTGGGGAGAACTCGACTTCCTCGTCGTCGACGCCCCTCCAGGGACATCAGACGAGCACATTTCAATCGTACAATGCCTCGAGGCTACCGGAATCGAAGGTGCAATAATTGTCACCACCCCACAACAAGTTTCTCTCATCGATGTGCGCAAAGAGGTGAGCTTCTGCAAAAAAGTCGGCGTCAAAGTCCTCGGAGTCGTAGAAAACATGAGCGGCTTGTGCCAACCGCTGTTGGATCTGAAGTTTCTAAAGACGAACAAGGCAGGAGAGCAGGCAGACGTAACGGAATGGGCAGCGGAGTACATCCGGGAGAAGGCGCCGGAGCTGCTAGATTTGATCACTTGCAGTGAAGTGTTTGATAGCAGTGGGGGAGGGGCTGCAAAAATGTGTAGGGAGATGGGGGTGCCATTTCTAGGGAAGGTGCCATTGGATCCACAGCTTTGTAAGGCAGCTGAAGAAGGAAGATCATGCTTTGGGGACCAGAAATGTGGGGTAAGTGCCCCAGCATTGAAGAACATTATAGAGAAGTTGATGGAAAATCAAGGGCTTTCAAGTTTCAACAATGTCAAT

mRNA sequence

GATTGCCCTGGTCCTCAGTCGGAGTCCGCGGGGAAATCCGACGCCTGCCAAGGATGTCCCAACCAAGAAATTTGTGCTACTGCTCCCAAAGGCCCTGACCCAGACCTGGTTGCGATAGCAGAACGAATGGCAACTGTTAAGCACAAGATTCTGGTTCTATCAGGGAAGGGTGGAGTCGGAAAGAGCACGTTCTCTGCTCAACTATCGTTTGCACTAGCAGCCATGGACTTCCAGGTAGGCCTCCTAGACATCGATATTTGTGGCCCAAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGCAACCTTGGATGGTCCCCTGTTTACGTAGAGTCCAACCTTGGAGTCATGTCAATAGGGTTCATGCTCCCAAACCCAGACGAAGCTGTCATTTGGAGAGGCCCTCGAAAAAACGGTCTCATCAAGCAGTTTCTAAAGGACGTGTACTGGGGAGAACTCGACTTCCTCGTCGTCGACGCCCCTCCAGGGACATCAGACGAGCACATTTCAATCGTACAATGCCTCGAGGCTACCGGAATCGAAGGTGCAATAATTGTCACCACCCCACAACAAGTTTCTCTCATCGATGTGCGCAAAGAGGTGAGCTTCTGCAAAAAAGTCGGCGTCAAAGTCCTCGGAGTCGTAGAAAACATGAGCGGCTTGTGCCAACCGCTGTTGGATCTGAAGTTTCTAAAGACGAACAAGGCAGGAGAGCAGGCAGACGTAACGGAATGGGCAGCGGAGTACATCCGGGAGAAGGCGCCGGAGCTGCTAGATTTGATCACTTGCAGTGAAGTGTTTGATAGCAGTGGGGGAGGGGCTGCAAAAATGTGTAGGGAGATGGGGGTGCCATTTCTAGGGAAGGTGCCATTGGATCCACAGCTTTGTAAGGCAGCTGAAGAAGGAAGATCATGCTTTGGGGACCAGAAATGTGGGGTAAGTGCCCCAGCATTGAAGAACATTATAGAGAAGTTGATGGAAAATCAAGGGCTTTCAAGTTTCAACAATGTCAAT

Coding sequence (CDS)

GATTGCCCTGGTCCTCAGTCGGAGTCCGCGGGGAAATCCGACGCCTGCCAAGGATGTCCCAACCAAGAAATTTGTGCTACTGCTCCCAAAGGCCCTGACCCAGACCTGGTTGCGATAGCAGAACGAATGGCAACTGTTAAGCACAAGATTCTGGTTCTATCAGGGAAGGGTGGAGTCGGAAAGAGCACGTTCTCTGCTCAACTATCGTTTGCACTAGCAGCCATGGACTTCCAGGTAGGCCTCCTAGACATCGATATTTGTGGCCCAAGTATCCCGAAGATGCTTGGTCTAGAAGGTCAAGATGTGCATCAGAGCAACCTTGGATGGTCCCCTGTTTACGTAGAGTCCAACCTTGGAGTCATGTCAATAGGGTTCATGCTCCCAAACCCAGACGAAGCTGTCATTTGGAGAGGCCCTCGAAAAAACGGTCTCATCAAGCAGTTTCTAAAGGACGTGTACTGGGGAGAACTCGACTTCCTCGTCGTCGACGCCCCTCCAGGGACATCAGACGAGCACATTTCAATCGTACAATGCCTCGAGGCTACCGGAATCGAAGGTGCAATAATTGTCACCACCCCACAACAAGTTTCTCTCATCGATGTGCGCAAAGAGGTGAGCTTCTGCAAAAAAGTCGGCGTCAAAGTCCTCGGAGTCGTAGAAAACATGAGCGGCTTGTGCCAACCGCTGTTGGATCTGAAGTTTCTAAAGACGAACAAGGCAGGAGAGCAGGCAGACGTAACGGAATGGGCAGCGGAGTACATCCGGGAGAAGGCGCCGGAGCTGCTAGATTTGATCACTTGCAGTGAAGTGTTTGATAGCAGTGGGGGAGGGGCTGCAAAAATGTGTAGGGAGATGGGGGTGCCATTTCTAGGGAAGGTGCCATTGGATCCACAGCTTTGTAAGGCAGCTGAAGAAGGAAGATCATGCTTTGGGGACCAGAAATGTGGGGTAAGTGCCCCAGCATTGAAGAACATTATAGAGAAGTTGATGGAAAATCAAGGGCTTTCAAGTTTCAACAATGTCAAT

Protein sequence

DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKAGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLCKAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFNNVN
Homology
BLAST of MS009793 vs. NCBI nr
Match: XP_022154194.1 (cytosolic Fe-S cluster assembly factor NBP35 isoform X1 [Momordica charantia])

HSP 1 Score: 695.7 bits (1794), Expect = 2.1e-196
Identity = 341/342 (99.71%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG
Sbjct: 21  DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 80

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV
Sbjct: 81  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 140

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE
Sbjct: 141 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 200

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA
Sbjct: 201 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 260

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC
Sbjct: 261 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 320

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFNNVN 343
           KAAEEGRSCFGDQ+CGVSAPALKNIIEKLMENQGLSSFNNVN
Sbjct: 321 KAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFNNVN 362

BLAST of MS009793 vs. NCBI nr
Match: XP_022154195.1 (cytosolic Fe-S cluster assembly factor NBP35 isoform X2 [Momordica charantia])

HSP 1 Score: 693.7 bits (1789), Expect = 7.8e-196
Identity = 340/342 (99.42%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA
Sbjct: 193 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC
Sbjct: 253 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFNNVN 343
           KAAEEGRSCFGDQ+CGVSAPALKNIIEKLMENQGLSSFNNVN
Sbjct: 313 KAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFNNVN 354

BLAST of MS009793 vs. NCBI nr
Match: XP_023530062.1 (cytosolic Fe-S cluster assembly factor NBP35 [Cucurbita pepo subsp. pepo] >KAG7022684.1 Cytosolic Fe-S cluster assembly factor NBP35 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 315/339 (92.92%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGL QPLLDLKFLKT + 
Sbjct: 193 ATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQPLLDLKFLKTTET 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
            EQ DVTEWAAEYIREKAPELL+LITCSEVFDSSGGGAAKMCR+MGVPFLGKVPLDPQLC
Sbjct: 253 EEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCRDMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKC VSAPALK IIE L+E+QGLS+ +
Sbjct: 313 KAAEEGRSCFGDQKCRVSAPALKKIIENLLESQGLSTMS 351

BLAST of MS009793 vs. NCBI nr
Match: XP_038883029.1 (cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida])

HSP 1 Score: 647.9 bits (1670), Expect = 4.9e-182
Identity = 313/339 (92.33%), Postives = 328/339 (96.76%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQL+FALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           A+GI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMS LCQPLLD+KFLKT +A
Sbjct: 193 ASGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSSLCQPLLDMKFLKTTEA 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQ DVTEW AEYIREKAPELL++I+CSEVFDSSGGGAA MCREM VPFLGKVPLDPQLC
Sbjct: 253 GEQTDVTEWVAEYIREKAPELLNVISCSEVFDSSGGGAANMCREMDVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKCGVSA ALK IIEKL++NQG S+ +
Sbjct: 313 KAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMS 351

BLAST of MS009793 vs. NCBI nr
Match: KAG6588917.1 (Cytosolic Fe-S cluster assembly factor NBP35, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 647.9 bits (1670), Expect = 4.9e-182
Identity = 314/339 (92.63%), Postives = 328/339 (96.76%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGL QPLLDLKFLK  + 
Sbjct: 193 ATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQPLLDLKFLKITET 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
            EQ DVTEWAAEYIREKAPELL+LITCSEVFDSSGGGAAKMCR+MGVPFLGKVPLDPQLC
Sbjct: 253 EEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCRDMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKC VSAPALK IIE L+E+QG +SF+
Sbjct: 313 KAAEEGRSCFGDQKCRVSAPALKKIIENLLESQGCTSFS 351

BLAST of MS009793 vs. ExPASy Swiss-Prot
Match: Q8H1Q2 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=NBP35 PE=1 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 1.4e-160
Identity = 274/330 (83.03%), Postives = 300/330 (90.91%), Query Frame = 0

Query: 2   CPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVGK 61
           CPGPQSESAGKSD+C GCPNQE CATAPKGPDPDLVAIAERM+TVKHKILVLSGKGGVGK
Sbjct: 14  CPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKHKILVLSGKGGVGK 73

Query: 62  STFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGVM 121
           STFSAQLSFALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLGWSPVYVE NLGVM
Sbjct: 74  STFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVEDNLGVM 133

Query: 122 SIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLEA 181
           SIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGTSDEHISIVQ L  
Sbjct: 134 SIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGTSDEHISIVQYLLP 193

Query: 182 TGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLK-TNKA 241
           TGI+GAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMSGL QPL D+KF+K   + 
Sbjct: 194 TGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQPLKDVKFMKLATET 253

Query: 242 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 301
           G   +VTE     +R+ APELLD++ CSEVFDSSGGGA +MCREMGVPFLGKVP+DPQLC
Sbjct: 254 GSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGVPFLGKVPMDPQLC 313

Query: 302 KAAEEGRSCFGDQKCGVSAPALKNIIEKLM 331
           KAAE+G+SCF D KC +SAPALK+II+K++
Sbjct: 314 KAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

BLAST of MS009793 vs. ExPASy Swiss-Prot
Match: Q8T2F3 (Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum OX=44689 GN=nubp1 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 1.5e-104
Identity = 184/309 (59.55%), Postives = 233/309 (75.40%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPG QSE +GKS AC GCPNQ+ICATAPKGPDPD++ I ERM TVK+KILVLSGKGGVG
Sbjct: 12  NCPGTQSEMSGKSAACAGCPNQQICATAPKGPDPDIIEIEERMKTVKNKILVLSGKGGVG 71

Query: 61  KSTFSAQLSFALAAMD-FQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLG 120
           KSTFS+QLSFAL+  +  +VGLLDIDICGPSIPK++GLEG+++H S  GW PVYV+ NL 
Sbjct: 72  KSTFSSQLSFALSMDEKVEVGLLDIDICGPSIPKIMGLEGENIHISGQGWDPVYVQDNLA 131

Query: 121 VMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCL 180
           VMS+GF+L   ++AVIWRGP+KNG+IKQFLKDVYW +LD+LV+D PPGTSDEH+SIVQ L
Sbjct: 132 VMSVGFLLEKEEDAVIWRGPKKNGIIKQFLKDVYWNDLDYLVIDTPPGTSDEHLSIVQYL 191

Query: 181 EATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNK 240
           + + + GA+IVT+PQ V+LIDVRKE++FCKKVGV ++GVVENMSG   P       K NK
Sbjct: 192 KTSNLSGAVIVTSPQDVALIDVRKEINFCKKVGVPIIGVVENMSGFVCP-------KCNK 251

Query: 241 AGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQL 300
                                       S++F  + GGA KM ++M VPFLG++P+DP +
Sbjct: 252 E---------------------------SQIFIPTTGGAEKMSQDMNVPFLGRIPIDPLI 286

Query: 301 CKAAEEGRS 309
            ++ +EG+S
Sbjct: 312 ARSCDEGKS 286

BLAST of MS009793 vs. ExPASy Swiss-Prot
Match: Q6P298 (Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=2 SV=2)

HSP 1 Score: 372.9 bits (956), Expect = 4.0e-102
Identity = 183/332 (55.12%), Postives = 234/332 (70.48%), Query Frame = 0

Query: 2   CPGPQSESAGKSDACQGCPNQEICAT-APKGPDPDLVAIAERMATVKHKILVLSGKGGVG 61
           CPG  S+ AGKS ACQGCPNQ ICA+ A K PDP +  I ++M +VKHKILVLSGKGGVG
Sbjct: 12  CPGTSSDQAGKSSACQGCPNQSICASGATKAPDPAIEEIKQKMTSVKHKILVLSGKGGVG 71

Query: 62  KSTFSAQLSFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLG 121
           KSTFSA LS ALA+    +V LLD+DICGPSIPK++GLEG+ VHQS  GWSPVYVE NL 
Sbjct: 72  KSTFSAHLSHALASDSSKEVALLDVDICGPSIPKIMGLEGEQVHQSGSGWSPVYVEDNLA 131

Query: 122 VMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCL 181
           VMSIGF+L +PD+AVIWRGP+KNG+IKQFL+DV WGE+D+L+VD PPGTSDEH+SIVQ L
Sbjct: 132 VMSIGFLLSSPDDAVIWRGPKKNGMIKQFLRDVDWGEVDYLIVDTPPGTSDEHLSIVQYL 191

Query: 182 EATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNK 241
              GI+GA+I+TTPQ+VSL DVRKE+ FCKKV + +LGV+ENMSG   P           
Sbjct: 192 SGAGIDGAVIITTPQEVSLQDVRKEIRFCKKVNLPILGVIENMSGFVCPKCK-------- 251

Query: 242 AGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQL 301
                                       S++F  + GGA +MC E+ +P LG++PLDP++
Sbjct: 252 --------------------------NTSQIFPPTTGGAQRMCEELNLPLLGRIPLDPRI 309

Query: 302 CKAAEEGRSCFGDQKCGVSAPALKNIIEKLME 332
            K+ +EG+S   +     +A A ++I++K+ +
Sbjct: 312 GKSCDEGKSFLTEVPDSPAAAAYQSIVQKIRD 309

BLAST of MS009793 vs. ExPASy Swiss-Prot
Match: Q6C7A6 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=NBP35 PE=3 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 5.2e-102
Identity = 179/334 (53.59%), Postives = 235/334 (70.36%), Query Frame = 0

Query: 2   CPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVGK 61
           CPG +SE AGK+DACQGCPNQ+ICA+APKGPDPDL  I +RM  VKHKILVLSGKGGVGK
Sbjct: 29  CPGTESEEAGKADACQGCPNQDICASAPKGPDPDLPLIKDRMKGVKHKILVLSGKGGVGK 88

Query: 62  STFSAQLSFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 121
           STFS+ L +  A+ +D +VGL+DIDICGPS+PKM+G EG+ +H S  GWSP+YV  NLG+
Sbjct: 89  STFSSLLGWGFASDLDREVGLMDIDICGPSLPKMMGSEGEQIHTSLSGWSPIYVSDNLGM 148

Query: 122 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 181
           MS+GFMLPN D+A+IWRG +KNGLIKQFLKDV WG LD+LVVD PPGTSDEH+S+ Q L+
Sbjct: 149 MSVGFMLPNQDDAIIWRGAKKNGLIKQFLKDVDWGNLDYLVVDTPPGTSDEHLSVTQYLK 208

Query: 182 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 241
            +G++GA+++TTPQ+V+L+DVRKE+ FC+K G+K++G+VENMSG         F+  N  
Sbjct: 209 ESGVDGAVVITTPQEVALLDVRKELDFCRKSGIKIIGLVENMSG---------FVCPNCK 268

Query: 242 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 301
           GE                         S +F  + GG   +  E  +PFLG VPLDP++ 
Sbjct: 269 GE-------------------------SFIFAPTTGGGKALAEEFNIPFLGSVPLDPRIG 328

Query: 302 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQG 335
           K+ + G S   +     +  A+ ++I ++ E  G
Sbjct: 329 KSCDHGESFVEEYPDSPATTAILDVIRQIREAVG 328

BLAST of MS009793 vs. ExPASy Swiss-Prot
Match: Q59MP1 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) OX=237561 GN=NBP35 PE=3 SV=3)

HSP 1 Score: 369.8 bits (948), Expect = 3.4e-101
Identity = 182/341 (53.37%), Postives = 240/341 (70.38%), Query Frame = 0

Query: 2   CPGPQSESAGKSDACQGCPNQEICAT-APKGPDPDLVAIAERMATVKHKILVLSGKGGVG 61
           CPGP+SE AG+ DAC+GC NQEIC++   KGPDPDL  I ER++ + HKILVLSGKGGVG
Sbjct: 21  CPGPESELAGQGDACKGCANQEICSSQTVKGPDPDLPIITERLSAIDHKILVLSGKGGVG 80

Query: 62  KSTFSAQLSFALAA-MDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLG 121
           KSTF++ L++A+AA  + +VG +D+DICGPS+P+MLG EG+ VHQSN GWSPVYV  NLG
Sbjct: 81  KSTFTSMLAWAIAADEEIEVGAMDLDICGPSLPRMLGAEGESVHQSNSGWSPVYVADNLG 140

Query: 122 VMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGE-LDFLVVDAPPGTSDEHISIVQC 181
           +MSI FMLP+PD A+IWRG +KNGLIKQFLKDV WGE LD+LVVD PPGTSDEH+S+   
Sbjct: 141 LMSISFMLPDPDSAIIWRGAKKNGLIKQFLKDVNWGEKLDYLVVDTPPGTSDEHLSVTTY 200

Query: 182 LEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTN 241
           ++  GI+GA+IVTTPQ+V+L+DVRKE+ FC+K  +K+LG+VENMSG         F+  N
Sbjct: 201 MKEVGIDGALIVTTPQEVALLDVRKEIDFCRKANIKILGLVENMSG---------FVCPN 260

Query: 242 KAGEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQ 301
             GE                         S++F ++ GG  ++C E+G+PFLG VPLDP+
Sbjct: 261 CKGE-------------------------SQIFKATTGGGKRLCEELGIPFLGSVPLDPR 320

Query: 302 LCKAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           + KA + G   F       +A A+ ++++ L +   LS  N
Sbjct: 321 IGKACDMGECFFDSYPDSPAATAILDVVDALRDQVELSLEN 327

BLAST of MS009793 vs. ExPASy TrEMBL
Match: A0A6J1DLB9 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=LOC111021512 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 9.9e-197
Identity = 341/342 (99.71%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG
Sbjct: 21  DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 80

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV
Sbjct: 81  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 140

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE
Sbjct: 141 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 200

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA
Sbjct: 201 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 260

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC
Sbjct: 261 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 320

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFNNVN 343
           KAAEEGRSCFGDQ+CGVSAPALKNIIEKLMENQGLSSFNNVN
Sbjct: 321 KAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFNNVN 362

BLAST of MS009793 vs. ExPASy TrEMBL
Match: A0A6J1DIY7 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=LOC111021512 PE=3 SV=1)

HSP 1 Score: 693.7 bits (1789), Expect = 3.8e-196
Identity = 340/342 (99.42%), Postives = 342/342 (100.00%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA
Sbjct: 193 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC
Sbjct: 253 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFNNVN 343
           KAAEEGRSCFGDQ+CGVSAPALKNIIEKLMENQGLSSFNNVN
Sbjct: 313 KAAEEGRSCFGDQECGVSAPALKNIIEKLMENQGLSSFNNVN 354

BLAST of MS009793 vs. ExPASy TrEMBL
Match: A0A6J1JQ09 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC111486691 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.1e-182
Identity = 314/339 (92.63%), Postives = 327/339 (96.46%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAGKSDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGKSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGL QPLLDLKFLK  + 
Sbjct: 193 ATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQPLLDLKFLKATET 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
            EQ DVTEWAAEYIREKAPELL+LITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC
Sbjct: 253 EEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKC VSAPALK IIE L+E+QG S+ +
Sbjct: 313 KAAEEGRSCFGDQKCRVSAPALKKIIENLIESQGFSTMS 351

BLAST of MS009793 vs. ExPASy TrEMBL
Match: A0A6J1ERE1 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LOC111435180 PE=3 SV=1)

HSP 1 Score: 647.1 bits (1668), Expect = 4.1e-182
Identity = 313/339 (92.33%), Postives = 328/339 (96.76%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGPQSESAG+SDACQGCPNQ ICATAPKGPDPDLV+IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPQSESAGRSDACQGCPNQSICATAPKGPDPDLVSIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQLSFALAAMDFQVGL+DIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLSFALAAMDFQVGLMDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPD+AVIWRGPRKNGLIKQFLKDVYWGELDFL+VDAPPGTSDEHISIVQCLE
Sbjct: 133 MSIGFMLPNPDDAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLE 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGI+GAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGL QPLLDLKFLKT + 
Sbjct: 193 ATGIDGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLTQPLLDLKFLKTTET 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
            EQ DVTEWAAEYIREKAPELL+LITCSEVFDSSGGGAAKMCR+MGVPFLGKVPLDPQLC
Sbjct: 253 EEQVDVTEWAAEYIREKAPELLNLITCSEVFDSSGGGAAKMCRDMGVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKC VSAPALK IIE L+E+QG S+ +
Sbjct: 313 KAAEEGRSCFGDQKCRVSAPALKKIIENLLESQGFSTMS 351

BLAST of MS009793 vs. ExPASy TrEMBL
Match: A0A1S3B5W7 (Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC103486120 PE=3 SV=1)

HSP 1 Score: 641.3 bits (1653), Expect = 2.2e-180
Identity = 310/339 (91.45%), Postives = 326/339 (96.17%), Query Frame = 0

Query: 1   DCPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVG 60
           +CPGP+SESAGKSDACQGCPNQ ICATAPKGPDPDL++IAERMATVKHKILVLSGKGGVG
Sbjct: 13  NCPGPESESAGKSDACQGCPNQSICATAPKGPDPDLISIAERMATVKHKILVLSGKGGVG 72

Query: 61  KSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGV 120
           KSTFSAQL+FALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVY+ESNLGV
Sbjct: 73  KSTFSAQLAFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYIESNLGV 132

Query: 121 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLE 180
           MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCL+
Sbjct: 133 MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLK 192

Query: 181 ATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLKTNKA 240
           ATGI+GAIIVTTPQQVSLIDVRKEVSFCK VGV+VLGVVENMS LCQPLLD+KFLKT +A
Sbjct: 193 ATGIDGAIIVTTPQQVSLIDVRKEVSFCKTVGVEVLGVVENMSSLCQPLLDMKFLKTTEA 252

Query: 241 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 300
           GEQ DVTEW  EYIREKAPELL+LIT SEVFDSSGGGAAKMCREM VPFLGKVPLDPQLC
Sbjct: 253 GEQTDVTEWVGEYIREKAPELLNLITYSEVFDSSGGGAAKMCREMDVPFLGKVPLDPQLC 312

Query: 301 KAAEEGRSCFGDQKCGVSAPALKNIIEKLMENQGLSSFN 340
           KAAEEGRSCFGDQKCGVSA ALK IIEKL++NQG S+ +
Sbjct: 313 KAAEEGRSCFGDQKCGVSASALKKIIEKLIQNQGFSTMS 351

BLAST of MS009793 vs. TAIR 10
Match: AT5G50960.1 (nucleotide binding protein 35 )

HSP 1 Score: 567.0 bits (1460), Expect = 1.0e-161
Identity = 274/330 (83.03%), Postives = 300/330 (90.91%), Query Frame = 0

Query: 2   CPGPQSESAGKSDACQGCPNQEICATAPKGPDPDLVAIAERMATVKHKILVLSGKGGVGK 61
           CPGPQSESAGKSD+C GCPNQE CATAPKGPDPDLVAIAERM+TVKHKILVLSGKGGVGK
Sbjct: 14  CPGPQSESAGKSDSCAGCPNQEACATAPKGPDPDLVAIAERMSTVKHKILVLSGKGGVGK 73

Query: 62  STFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDVHQSNLGWSPVYVESNLGVM 121
           STFSAQLSFALA MD QVGL+DIDICGPSIPKMLGLEGQ++HQSNLGWSPVYVE NLGVM
Sbjct: 74  STFSAQLSFALAGMDHQVGLMDIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVEDNLGVM 133

Query: 122 SIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVVDAPPGTSDEHISIVQCLEA 181
           SIGFMLPN DEAVIWRGPRKNGLIKQFLKDVYWGE+D+LVVDAPPGTSDEHISIVQ L  
Sbjct: 134 SIGFMLPNSDEAVIWRGPRKNGLIKQFLKDVYWGEIDYLVVDAPPGTSDEHISIVQYLLP 193

Query: 182 TGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENMSGLCQPLLDLKFLK-TNKA 241
           TGI+GAIIVTTPQ+VSLIDVRKEVSFCKKVGV VLGVVENMSGL QPL D+KF+K   + 
Sbjct: 194 TGIDGAIIVTTPQEVSLIDVRKEVSFCKKVGVPVLGVVENMSGLSQPLKDVKFMKLATET 253

Query: 242 GEQADVTEWAAEYIREKAPELLDLITCSEVFDSSGGGAAKMCREMGVPFLGKVPLDPQLC 301
           G   +VTE     +R+ APELLD++ CSEVFDSSGGGA +MCREMGVPFLGKVP+DPQLC
Sbjct: 254 GSSINVTEDVIACLRKNAPELLDIVACSEVFDSSGGGAERMCREMGVPFLGKVPMDPQLC 313

Query: 302 KAAEEGRSCFGDQKCGVSAPALKNIIEKLM 331
           KAAE+G+SCF D KC +SAPALK+II+K++
Sbjct: 314 KAAEQGKSCFEDNKCLISAPALKSIIQKVV 343

BLAST of MS009793 vs. TAIR 10
Match: AT4G19540.1 (IND1(iron-sulfur protein required for NADH dehydrogenase)-like )

HSP 1 Score: 141.7 bits (356), Expect = 1.1e-33
Identity = 83/185 (44.86%), Postives = 113/185 (61.08%), Query Frame = 0

Query: 42  RMATVKHKILVLSGKGGVGKSTFSAQLSFALA-AMDFQVGLLDIDICGPSIPKMLGLEGQ 101
           R+  VK  I V SGKGGVGKS+ +  L+ ALA   + ++GLLD D+ GPS+P M+ +  +
Sbjct: 38  RLHGVKDIIAVASGKGGVGKSSTAVNLAVALANKCELKIGLLDADVYGPSVPIMMNINQK 97

Query: 102 DVHQSNLGWSPVYVESNLGV--MSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELD 161
                ++   PV    N GV  MS+G +L   D  ++WRGP     + +  K V WG+LD
Sbjct: 98  PQVNQDMKMIPV---ENYGVKCMSMG-LLVEKDAPLVWRGPMVMSALAKMTKGVDWGDLD 157

Query: 162 FLVVDAPPGTSDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGV 221
            LVVD PPGT D  ISI Q L+   + GA+IV+TPQ V+L D  + +S   KV V +LG+
Sbjct: 158 ILVVDMPPGTGDAQISISQNLK---LSGAVIVSTPQDVALADANRGISMFDKVRVPILGL 215

Query: 222 VENMS 224
           VENMS
Sbjct: 218 VENMS 215

BLAST of MS009793 vs. TAIR 10
Match: AT3G24430.1 (ATP binding )

HSP 1 Score: 128.6 bits (322), Expect = 9.3e-30
Identity = 71/180 (39.44%), Postives = 104/180 (57.78%), Query Frame = 0

Query: 43  MATVKHKILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQDV 102
           ++ + + I V S KGGVGKST +  L++ LA M  +VG+ D D+ GPS+P M+  E + +
Sbjct: 172 LSRISNIIAVSSCKGGVGKSTVAVNLAYTLAGMGARVGIFDADVYGPSLPTMVNPESR-I 231

Query: 103 HQSNLGWSPVYVESNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLVV 162
            + N     +     +GV  + F       A++ RGP  +G+I Q L    WGELD+LV+
Sbjct: 232 LEMNPEKKTIIPTEYMGVKLVSFGFAGQGRAIM-RGPMVSGVINQLLTTTEWGELDYLVI 291

Query: 163 DAPPGTSDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVVENM 222
           D PPGT D  +++ Q    T    A+IVTTPQ+++ IDV K V    K+ V  + VVENM
Sbjct: 292 DMPPGTGDIQLTLCQVAPLT---AAVIVTTPQKLAFIDVAKGVRMFSKLKVPCVAVVENM 346

BLAST of MS009793 vs. TAIR 10
Match: AT5G24020.1 (septum site-determining protein (MIND) )

HSP 1 Score: 55.5 bits (132), Expect = 1.0e-07
Identity = 47/182 (25.82%), Postives = 82/182 (45.05%), Query Frame = 0

Query: 50  ILVLSGKGGVGKSTFSAQLSFALAAMDFQVGLLDIDICGPSIPKMLGLEGQ------DVH 109
           +++ SGKGGVGK+T +A +  +LA   F V  +D D+   ++  +LGLE +      +V 
Sbjct: 61  VVITSGKGGVGKTTTTANVGLSLARYGFSVVAIDADLGLRNLDLLLGLENRVNYTCVEVI 120

Query: 110 QSNLGWSPVYVE----SNLGVMSIGFMLPNPDEAVIWRGPRKNGLIKQFLKDVYWGELDF 169
             +       V     SN  ++ I    P     + + G     L+   LK    G  DF
Sbjct: 121 NGDCRLDQALVRDKRWSNFELLCIS--KPRSKLPMGFGGKALEWLV-DALKTRPEGSPDF 180

Query: 170 LVVDAPPGTSDEHISIVQCLEATGIEGAIIVTTPQQVSLIDVRKEVSFCKKVGVKVLGVV 222
           +++D P G     I+ +     T    A++VTTP   +L D  +     +  G++ + ++
Sbjct: 181 IIIDCPAGIDAGFITAI-----TPANEAVLVTTPDITALRDADRVTGLLECDGIRDIKMI 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154194.12.1e-19699.71cytosolic Fe-S cluster assembly factor NBP35 isoform X1 [Momordica charantia][more]
XP_022154195.17.8e-19699.42cytosolic Fe-S cluster assembly factor NBP35 isoform X2 [Momordica charantia][more]
XP_023530062.11.3e-18292.92cytosolic Fe-S cluster assembly factor NBP35 [Cucurbita pepo subsp. pepo] >KAG70... [more]
XP_038883029.14.9e-18292.33cytosolic Fe-S cluster assembly factor NBP35 [Benincasa hispida][more]
KAG6588917.14.9e-18292.63Cytosolic Fe-S cluster assembly factor NBP35, partial [Cucurbita argyrosperma su... [more]
Match NameE-valueIdentityDescription
Q8H1Q21.4e-16083.03Cytosolic Fe-S cluster assembly factor NBP35 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q8T2F31.5e-10459.55Cytosolic Fe-S cluster assembly factor NUBP1 homolog OS=Dictyostelium discoideum... [more]
Q6P2984.0e-10255.12Cytosolic Fe-S cluster assembly factor nubp1 OS=Danio rerio OX=7955 GN=nubp1 PE=... [more]
Q6C7A65.2e-10253.59Cytosolic Fe-S cluster assembly factor NBP35 OS=Yarrowia lipolytica (strain CLIB... [more]
Q59MP13.4e-10153.37Cytosolic Fe-S cluster assembly factor NBP35 OS=Candida albicans (strain SC5314 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DLB99.9e-19799.71Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1DIY73.8e-19699.42Cytosolic Fe-S cluster assembly factor NBP35 OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1JQ094.1e-18292.63Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1ERE14.1e-18292.33Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A1S3B5W72.2e-18091.45Cytosolic Fe-S cluster assembly factor NBP35 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
Match NameE-valueIdentityDescription
AT5G50960.11.0e-16183.03nucleotide binding protein 35 [more]
AT4G19540.11.1e-3344.86IND1(iron-sulfur protein required for NADH dehydrogenase)-like [more]
AT3G24430.19.3e-3039.44ATP binding [more]
AT5G24020.11.0e-0725.82septum site-determining protein (MIND) [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 42..337
e-value: 7.5E-88
score: 296.6
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 33..309
IPR033756Flagellum site-determining protein YlxH/ Fe-S cluster assembling factor NBP35PFAMPF10609ParAcoord: 267..329
e-value: 1.2E-6
score: 28.2
coord: 46..229
e-value: 7.8E-82
score: 274.4
NoneNo IPR availablePANTHERPTHR23264:SF36CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NBP35coord: 1..331
NoneNo IPR availableCDDcd02037Mrp_NBP35coord: 48..301
e-value: 3.10649E-110
score: 317.906
IPR019591Mrp/NBP35 ATP-binding proteinPANTHERPTHR23264NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATEDcoord: 1..331
IPR019591Mrp/NBP35 ATP-binding proteinHAMAPMF_02040Mrp_NBP35coord: 41..330
score: 35.943527
IPR000808Mrp, conserved sitePROSITEPS01215MRPcoord: 154..170
IPR028601Cytosolic Fe-S cluster assembly factor NUBP1/Nbp35HAMAPMF_03038NUBP1coord: 1..334
score: 46.996552

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009793.1MS009793.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0051536 iron-sulfur cluster binding