Homology
BLAST of MS009726 vs. NCBI nr
Match:
XP_022141072.1 (subtilisin-like protease SBT3.9 [Momordica charantia])
HSP 1 Score: 1409.8 bits (3648), Expect = 0.0e+00
Identity = 709/736 (96.33%), Postives = 711/736 (96.60%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDT GIWP
Sbjct: 101 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF
Sbjct: 161 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV
Sbjct: 221 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG
Sbjct: 281 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV ++ E
Sbjct: 341 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
ACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK
Sbjct: 401 VSDLEDERACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN
Sbjct: 461 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG
Sbjct: 641 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL
Sbjct: 701 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIPISVRTDI
Sbjct: 761 DGVHSVRIPISVRTDI 775
BLAST of MS009726 vs. NCBI nr
Match:
KAG7014677.1 (Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1243.0 bits (3215), Expect = 0.0e+00
Identity = 618/736 (83.97%), Postives = 666/736 (90.49%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHKMQTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 101 MISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 161 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 221 QDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 281 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+ ++ E
Sbjct: 341 NSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWAAGNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK
Sbjct: 401 VSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPFIN+FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 461 AVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYNDSAISGITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVG
Sbjct: 641 DYIQYFCAKGYNDSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IA+KPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWL
Sbjct: 701 AINSSYKAAIEAP--PGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 761 DGVHSVRIPVSVRTNI 773
BLAST of MS009726 vs. NCBI nr
Match:
KAG6576624.1 (Subtilisin-like protease 3.3, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1240.7 bits (3209), Expect = 0.0e+00
Identity = 616/736 (83.70%), Postives = 666/736 (90.49%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 25 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 84
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHKMQTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 85 MISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 144
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 145 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEF 204
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 205 QDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 264
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 265 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 324
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+ ++ E
Sbjct: 325 NSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPE 384
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWAAGNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK
Sbjct: 385 VSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTK 444
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPF+N+FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 445 AVEPFVNNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 504
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 505 SVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHW 564
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 565 SPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 624
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYN+SAISGITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVG
Sbjct: 625 DYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVG 684
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IA+KPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWL
Sbjct: 685 AINSSYKAAIEAP--PGITIAIKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWL 744
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 745 DGVHSVRIPVSVRTNI 757
BLAST of MS009726 vs. NCBI nr
Match:
XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])
HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 617/736 (83.83%), Postives = 665/736 (90.35%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHKMQTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 101 MISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 161 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 221 QDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 281 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVD SFLVAITLGNNWTT+ ++ E
Sbjct: 341 NSGPAAYSVSNIEPWLLTVAASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWAAGNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK
Sbjct: 401 VSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPFIN+FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 461 AVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYN+SAISGITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVG
Sbjct: 641 DYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IAVKPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWL
Sbjct: 701 AINSSYKAAIEAP--PGITIAVKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 761 DGVHSVRIPVSVRTNI 773
BLAST of MS009726 vs. NCBI nr
Match:
XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 616/736 (83.70%), Postives = 664/736 (90.22%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHK+QTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 101 MISELPSVVEVIPNRLHKIQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 161 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSKF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 221 QDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 281 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+ ++ E
Sbjct: 341 NSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWAAGNVVLCFASDDFND+TH+TS SVKKVG LGLIVAKNPTK
Sbjct: 401 VSDLDDERACESLSLNDTWAAGNVVLCFASDDFNDDTHNTSSSVKKVGGLGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPFIN+FPCVQ+SL+ GM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 461 AVEPFINNFPCVQISLDTGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYN+SAISGITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVG
Sbjct: 641 DYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IAVKPR+LKFNHK K I FTVT+SSNHRVTTGYCFGSLTWL
Sbjct: 701 AINSSYKAAIEAP--PGITIAVKPRILKFNHKMKSIPFTVTISSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 761 DGVHSVRIPVSVRTNI 773
BLAST of MS009726 vs. ExPASy Swiss-Prot
Match:
Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)
HSP 1 Score: 788.1 bits (2034), Expect = 8.5e-227
Identity = 408/744 (54.84%), Postives = 525/744 (70.56%), Query Frame = 0
Query: 7 QVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQA 66
+V++VY+G++ H + E + ++HH +LAS+LGS++ DSMVY+Y++GFSGFAAKLT +QA
Sbjct: 31 KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQA 90
Query: 67 QKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIW 126
+KI++LP V+ VIP+ H++ TTR+W+YL LS +LL + MG+ IIG++DT G+W
Sbjct: 91 KKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVIDT-GVW 150
Query: 127 PESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSG 186
PESE F+DNG+GP+P +WKG CESGE F C+RKLIGA+YFI G A +N +
Sbjct: 151 PESESFNDNGVGPIPRKWKGGCESGENFR-STDCNRKLIGAKYFINGF-LAENKGFNTTE 210
Query: 187 FQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCW---QL 246
+DY+S RD+ GHGTHV+S+A GSFV NVSY GLA GT+RGGAP++R+AMYK CW +L
Sbjct: 211 SRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACWFHEEL 270
Query: 247 NGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVV 306
G CSD+DI+KAID+AIHDGVDVLS+SL P S+ D+ + A G FHA+ KGIVVV
Sbjct: 271 KGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAKGIVVV 330
Query: 307 GAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTT------------VVIL 366
A GN GPAA +V NI PW+LTVAA+++DRSF ITLGNN + L
Sbjct: 331 CAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPELGLTSL 390
Query: 367 IEPHHANSNY-----ACESLSLNDTWA-AGNVVLCFASDDFNDNTHHTSRSVKKVGALGL 426
+ P +A +N CESL+LN + A VVLCF + N + VK G LGL
Sbjct: 391 VYPENARNNNETFSGVCESLNLNPNYTMAMKVVLCFTASRTNAAISRAASFVKAAGGLGL 450
Query: 427 IVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVA 486
I+++NP + P + FPCV V E+G IL+YIRSTR+P V I SRT GQ + T V
Sbjct: 451 IISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQPVGTKVV 510
Query: 487 YFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVAL 546
FSSRGPNS++PAILKPDIAAPGV ILAA P+D + +A +SGTSMATP +S ++AL
Sbjct: 511 NFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVISGVIAL 570
Query: 547 LKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPG 606
LK+LH WSPAA +SAIVTTAW +DP+GE IFAEG K + PFD+GGGIVNP KA +PG
Sbjct: 571 LKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEPG 630
Query: 607 LVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTV 666
L+YDMG DYI Y C+ GYNDS+IS + + C +PS+LD N+PSITIP LK VT+
Sbjct: 631 LIYDMGPQDYILYLCSAGYNDSSISQLVGQITVCSNPKPSVLDVNLPSITIPNLKDEVTL 690
Query: 667 TRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGY 726
TRTVTNVG V+S YK ++E P G+ + V P L FN K +SFTV VS+ H++ TGY
Sbjct: 691 TRTVTNVGLVDSVYKVSVEPP--LGVRVVVTPETLVFNSKTISVSFTVRVSTTHKINTGY 750
Query: 727 CFGSLTWLDGVHSVRIPISVRTDI 730
FGSLTW D VH+V IP+SVRT I
Sbjct: 751 YFGSLTWTDSVHNVVIPLSVRTQI 769
BLAST of MS009726 vs. ExPASy Swiss-Prot
Match:
Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)
HSP 1 Score: 785.8 bits (2028), Expect = 4.2e-226
Identity = 415/742 (55.93%), Postives = 528/742 (71.16%), Query Frame = 0
Query: 1 FLLGAQQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAK 60
F++ +VYVVY+G++ HDN E + ++HH +L S+LGS+EA DS+VY+Y++GFSGFAAK
Sbjct: 22 FVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAK 81
Query: 61 LTMAQAQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLL 120
LT +QAQ+ISELP V++VIPN L++M TTR+WDYL +SP +SLLQK+ MG I+G++
Sbjct: 82 LTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVI 141
Query: 121 DTAGIWPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGH 180
D +G+WPESE+F+D G GP+PSRWKG CESGELF+ C+RKLIGA+YF+ GL A +G
Sbjct: 142 D-SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG- 201
Query: 181 PYNNSGFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVC 240
N + +YLSPRD+AGHGTHV+S GSF+ NVSY GL GT RGGAP +A+YK C
Sbjct: 202 VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKAC 261
Query: 241 WQLNGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGI 300
W G CS AD+LKA+D+AIHDGVD+LSLSLGP+ P + + + ++G FHA+ KGI
Sbjct: 262 W---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGI 321
Query: 301 VVVGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV------------ 360
VV AAGN GP A +++N+ PW+LTVAA++ DRSF AITLGNN T +
Sbjct: 322 PVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGF 381
Query: 361 VILIEPHHANSNYACESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIV 420
V L P S CE LS N ++ G VVLCFA+ + ++ +V G LGLI+
Sbjct: 382 VGLTYPESPLSG-DCEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 441
Query: 421 AKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYF 480
AKNPT + P FP V + E+G IL YIRSTR+P V I S+T GQS+ST VA F
Sbjct: 442 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 501
Query: 481 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLK 540
SSRGPNSV+PAILKPDIAAPGV ILAA+ P+ + +A +SGTSMATP VS +V LLK
Sbjct: 502 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 561
Query: 541 SLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLV 600
SLH WSP+AIKSAIVTTAW +DP GE IFA+G K A PFD+GGG++NP KAV PGL+
Sbjct: 562 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 621
Query: 601 YDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTR 660
YDM DY+ Y C++ Y+D +IS + K CP +PS+LD N+PSITIP L+ VT+TR
Sbjct: 622 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 681
Query: 661 TVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCF 720
TVTNVG VNS YK I+ P GI +AV P L F++ SFTV VS+ H+V TGY F
Sbjct: 682 TVTNVGPVNSVYKVVIDPP--TGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYF 741
Query: 721 GSLTWLDGVHSVRIPISVRTDI 730
GSLTW D +H+V IP+SVRT I
Sbjct: 742 GSLTWTDNMHNVAIPVSVRTQI 748
BLAST of MS009726 vs. ExPASy Swiss-Prot
Match:
Q9ZSB1 (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3 SV=2)
HSP 1 Score: 779.2 bits (2011), Expect = 3.9e-224
Identity = 416/742 (56.06%), Postives = 523/742 (70.49%), Query Frame = 0
Query: 1 FLLGAQQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAK 60
F++ +VYVVY+G++ HDN E + ++HH +L S+LGS+EA DS+VY+Y++GFSGFAAK
Sbjct: 22 FVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAK 81
Query: 61 LTMAQAQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLL 120
LT +QAQ+ISELP V++VIPN L++M TTR+WDYL +SP +SLLQK+ MG I+G++
Sbjct: 82 LTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVI 141
Query: 121 DTAGIWPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGH 180
DT G+WPESE+F+D G GP+PSRWKG CESGELF+ C+RKLIGA+YFI A +G
Sbjct: 142 DT-GVWPESEMFNDKGYGPIPSRWKGGCESGELFNGSIHCNRKLIGAKYFIDANNAQFG- 201
Query: 181 PYNNSGFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVC 240
N + DYLSPRD+ GHGTHV+S GSF+ NVSY GL GT RGGAP +A+YK C
Sbjct: 202 VLNKTENPDYLSPRDFNGHGTHVASTIGGSFLPNVSYLGLGRGTARGGAPGVHIAVYKAC 261
Query: 241 WQLNGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGI 300
W G CS AD+LKA+D+AIHDGVD+LSLSL + P + + D ++G FHA+ KGI
Sbjct: 262 WVQRG--CSGADVLKAMDEAIHDGVDILSLSLQTSVPLFPETDARELTSVGAFHAVAKGI 321
Query: 301 VVVGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV------------ 360
VV AA N GP A +++N+ PW+LTVAA++ DRSF AITLGNN T +
Sbjct: 322 PVVAAASNAGPTAQTLSNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIFGGSELGF 381
Query: 361 VILIEPHHANSNYACESLSLNDTWA-AGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIV 420
V L P S CE LS N A G VVLCFA+ + ++ +V G LGLI+
Sbjct: 382 VGLTYPESPLSG-DCEKLSANPKSAMEGKVVLCFAA---STPSNAAITAVINAGGLGLIM 441
Query: 421 AKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYF 480
A+NPT + P + +FP V V E+G IL YIRSTR+P VNI SRT GQS+ST VA F
Sbjct: 442 ARNPTHLLRP-LRNFPYVSVDFELGTDILFYIRSTRSPIVNIQASRTLFGQSVSTKVATF 501
Query: 481 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLK 540
SSRGPNSV+PAILKPDIAAPGV ILAA+ P+ D +A +SGTSMATP VS +V LLK
Sbjct: 502 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSIND-GGFAMMSGTSMATPVVSGVVVLLK 561
Query: 541 SLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLV 600
SLH WSP+AIKSAIVTTAW +DP GE IFA+G K A PFD+GGG++NP KAV PGL+
Sbjct: 562 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 621
Query: 601 YDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTR 660
YDM DY+ Y C++ Y+D +IS + K CP +PS+LD N+PSITIP L+ VT+TR
Sbjct: 622 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 681
Query: 661 TVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCF 720
TVTNVG VNS YK I+ P G+ +AV P L F+ SFTV VS+ H+V TGY F
Sbjct: 682 TVTNVGPVNSVYKVVIDPP--TGVNVAVTPTELVFDSTTTKRSFTVRVSTTHKVNTGYYF 741
Query: 721 GSLTWLDGVHSVRIPISVRTDI 730
GSLTW D +H+V IP+SVRT I
Sbjct: 742 GSLTWTDTLHNVAIPVSVRTQI 751
BLAST of MS009726 vs. ExPASy Swiss-Prot
Match:
Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)
HSP 1 Score: 774.2 bits (1998), Expect = 1.3e-222
Identity = 391/745 (52.48%), Postives = 526/745 (70.60%), Query Frame = 0
Query: 6 QQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQ 65
++V++VY+G++ HD+ E + ++HH +L S+LGS+E DSMVY+Y++GFSGFAAKLT +Q
Sbjct: 32 RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQ 91
Query: 66 AQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGI 125
A+KI++LP V+ VIP+ +K+ TTR+WDYL LS SLL ++ MG IIG++DT G+
Sbjct: 92 AKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT-GV 151
Query: 126 WPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNS 185
WPESEVF+D+G GPVPS WKG CE+GE F+ C++KLIGA+YFI G A +N++
Sbjct: 152 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFNST 211
Query: 186 GFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNG 245
D++SPRD GHGTHVS++A GSFV N+SY GLA GTVRGGAP++ +AMYK CW L+
Sbjct: 212 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 271
Query: 246 G---VCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVV 305
CS ADILKA+D+A+HDGVDVLS+SLG + P Y + D+ +G+ G FHA++KGI V
Sbjct: 272 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 331
Query: 306 VGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNN---------------WTT 365
V + GN GP + +V N PW++TVAA+++DRSF +TLGNN +T+
Sbjct: 332 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTS 391
Query: 366 VVILIEPHHANSNYA--CESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALG 425
+V P ++N +++ CE L N + G VVLCF + + +R VK+ G LG
Sbjct: 392 LVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGGLG 451
Query: 426 LIVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTV 485
+I+A++P ++P ++ FPCV V E+G IL Y RS+ +P V I PS+T +GQ + T V
Sbjct: 452 VIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKV 511
Query: 486 AYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVA 545
A FSSRGPNS+APAILKPDIAAPGV+ILAA + D+ + +SGTSMA P +S + A
Sbjct: 512 ATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGVAA 571
Query: 546 LLKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDP 605
LLK+LH WSPAAI+SAIVTTAW +DP+GE IFAEG P K A PFD+GGG+VNP K+ +P
Sbjct: 572 LLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANP 631
Query: 606 GLVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVT 665
GLVYDMG+ DY+ Y C++GYN+++IS + K C +PS+LD N+PSITIP LK VT
Sbjct: 632 GLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVT 691
Query: 666 VTRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTG 725
+TRTVTNVG +NS Y+ +E P G + V P L FN K + F V VS+ H+ TG
Sbjct: 692 ITRTVTNVGPLNSVYRVTVEPP--LGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 751
Query: 726 YCFGSLTWLDGVHSVRIPISVRTDI 730
Y FGSLTW D +H+V IP+SVRT I
Sbjct: 752 YYFGSLTWSDSLHNVTIPLSVRTQI 770
BLAST of MS009726 vs. ExPASy Swiss-Prot
Match:
Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)
HSP 1 Score: 773.5 bits (1996), Expect = 2.2e-222
Identity = 396/743 (53.30%), Postives = 530/743 (71.33%), Query Frame = 0
Query: 7 QVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQA 66
+V++VY+G++ HD+ E + ++HH +L S+LGS+E SMV+++++GFSGFAAKLT +QA
Sbjct: 33 KVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQA 92
Query: 67 QKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIW 126
+KI++LP V+ VIP+R +K TTR+WDYL LSP +LL ++ MG IIG++D +G+W
Sbjct: 93 KKIADLPEVVHVIPDRFYKPATTRTWDYLGLSPTNPKNLLNQTNMGEQMIIGIID-SGVW 152
Query: 127 PESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSG 186
PESEVF+DN +GPVPS WKG CESGE F+ C++KLIGA+YFI A + +N+S
Sbjct: 153 PESEVFNDNEIGPVPSHWKGGCESGEDFN-SSHCNKKLIGAKYFINAFLATH-ESFNSSE 212
Query: 187 FQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGG 246
D++SPR Y GHGTHV+++A GS+V N SY GLA GTVRGGAP++R+A+YK CW L+
Sbjct: 213 SLDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLD 272
Query: 247 V--CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVG 306
+ CS ADILKA+D+AIHDGVDVLSLSLG P Y + D+ +G+A G FHA++KGI VV
Sbjct: 273 IAACSSADILKAMDEAIHDGVDVLSLSLG-FEPLYPETDVRDGIATGAFHAVLKGITVVC 332
Query: 307 AAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNN---------------WTTVV 366
AAGN GPAA +V N PW+LTVAA+++DRSF+ +TLGNN +T++V
Sbjct: 333 AAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLV 392
Query: 367 ILIEPHHANSNYA--CESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLI 426
P ++N +++ CE L +N + AG VVLCF ++ + + VK+ G LG+I
Sbjct: 393 YPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVI 452
Query: 427 VAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAY 486
+A P + P ++ FPCV V E+G +IL YIRS +P V I PSRT IGQ + T VA
Sbjct: 453 IAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVAS 512
Query: 487 FSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALL 546
FSSRGPN ++ AILKPDIAAPGV+ILAA + + + F+SGTSMATP +S IVALL
Sbjct: 513 FSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALL 572
Query: 547 KSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGL 606
K+LH WSPAAI+SAIVTTAW +DP+GE IFAEG P K A PFD+GGG+VNP KA PGL
Sbjct: 573 KALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGL 632
Query: 607 VYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVT 666
VYD+G+ DY+ Y C++GYN+++IS + K C +PS+LD N+PSITIP LK VT+
Sbjct: 633 VYDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLP 692
Query: 667 RTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYC 726
RT+TNVG + S Y+ A+E P G + V P L FN K +SF V+VS+ H++ TGY
Sbjct: 693 RTLTNVGPLESVYRVAVEPP--LGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYY 752
Query: 727 FGSLTWLDGVHSVRIPISVRTDI 730
FGSLTW D +H+V IP+SVRT +
Sbjct: 753 FGSLTWSDSLHNVTIPLSVRTQL 769
BLAST of MS009726 vs. ExPASy TrEMBL
Match:
A0A6J1CIU2 (subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 PE=3 SV=1)
HSP 1 Score: 1409.8 bits (3648), Expect = 0.0e+00
Identity = 709/736 (96.33%), Postives = 711/736 (96.60%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDT GIWP
Sbjct: 101 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF
Sbjct: 161 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV
Sbjct: 221 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG
Sbjct: 281 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV ++ E
Sbjct: 341 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
ACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK
Sbjct: 401 VSDLEDERACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN
Sbjct: 461 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG
Sbjct: 641 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL
Sbjct: 701 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIPISVRTDI
Sbjct: 761 DGVHSVRIPISVRTDI 775
BLAST of MS009726 vs. ExPASy TrEMBL
Match:
A0A6J1E757 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)
HSP 1 Score: 1238.4 bits (3203), Expect = 0.0e+00
Identity = 617/736 (83.83%), Postives = 665/736 (90.35%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHKMQTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 101 MISELPSVVEVIPNRLHKMQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 161 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDY SPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 221 QDYWSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 281 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVD SFLVAITLGNNWTT+ ++ E
Sbjct: 341 NSGPAAYSVSNIEPWLLTVAASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWAAGNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK
Sbjct: 401 VSDLDDERACESLSLNDTWAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPFIN+FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 461 AVEPFINNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWSSDPYGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYN+SAISGITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVG
Sbjct: 641 DYIQYFCAKGYNNSAISGITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IAVKPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWL
Sbjct: 701 AINSSYKAAIEAP--PGITIAVKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 761 DGVHSVRIPVSVRTNI 773
BLAST of MS009726 vs. ExPASy TrEMBL
Match:
A0A6J1J508 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)
HSP 1 Score: 1237.2 bits (3200), Expect = 0.0e+00
Identity = 616/736 (83.70%), Postives = 667/736 (90.62%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
VY+VYMG++PHDNEELLVK HHGVLASVLGSQE DS+VY+YKYGFSGFAAKLTMAQAQ
Sbjct: 41 VYIVYMGQKPHDNEELLVKAHHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQ 100
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
ISELP+V++VIPNRLHK+QTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWP
Sbjct: 101 MISELPSVVEVIPNRLHKIQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWP 160
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS F
Sbjct: 161 ESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEF 220
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
QDYLSPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGV
Sbjct: 221 QDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGV 280
Query: 248 CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAG 307
C+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAG
Sbjct: 281 CADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAG 340
Query: 308 NYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV--------------VILIE 367
N GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+ ++ E
Sbjct: 341 NSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPE 400
Query: 368 PHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTK 427
+ ACESLSLNDTWA+GNVVLCFASDD+NDNTH+TS SVKKVG LGLIVAKNPTK
Sbjct: 401 VSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTK 460
Query: 428 DVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPN 487
VEPFI++FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPN
Sbjct: 461 AVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPN 520
Query: 488 SVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHW 547
SVAPAILKPDIAAPGVAILAAVPPSDPK++NSYAFISGTSMATPHVSAIVALLK+LHSHW
Sbjct: 521 SVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHW 580
Query: 548 SPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMA 607
SPAAIKSAIVTTAW+SDPYG IFAEGRP+ SA PFDFGGGIVNPNKAVDPGLVYDMGMA
Sbjct: 581 SPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMA 640
Query: 608 DYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVG 667
DYI+YFCA GYN+SAISGI+K+ ISCPKRRPSILD NVPSITIP L HSV++TRTVTNVG
Sbjct: 641 DYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVG 700
Query: 668 AVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWL 727
A+NS+YKAAIE P PGI IAVKPR+LKFNHK K ISFTVT+SSN RVTTGYCFGSLTWL
Sbjct: 701 AINSSYKAAIEVP--PGITIAVKPRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWL 760
Query: 728 DGVHSVRIPISVRTDI 730
DGVHSVRIP+SVRT+I
Sbjct: 761 DGVHSVRIPVSVRTNI 773
BLAST of MS009726 vs. ExPASy TrEMBL
Match:
A0A6J1E3V3 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)
HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 552/658 (83.89%), Postives = 592/658 (89.97%), Query Frame = 0
Query: 86 MQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWPESEVFSDNGLGPVPSRWK 145
MQTTRSWDYLQLSPR NSLL+KSRMG+GAIIG+LDT GIWPESEVF D GLGPVPSRWK
Sbjct: 1 MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDT-GIWPESEVFCDEGLGPVPSRWK 60
Query: 146 GICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSS 205
GICESGELFSP KACSRKLIGARYF+KGLEAAYGHP NNS FQDY SPRD +GHGTHVSS
Sbjct: 61 GICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSS 120
Query: 206 VASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGV 265
VASG FV NVSYHGLAVGTVRGGAP SR+AMYKVCWQ+NGGVC+D DILKAIDQAI+DGV
Sbjct: 121 VASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGV 180
Query: 266 DVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLT 325
DVLSLSLGP+FPSYSDVDM NG+AIG FHA+ KGIVVVGAAGN GPAAYSV+NIEPWLLT
Sbjct: 181 DVLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLT 240
Query: 326 VAASSVDRSFLVAITLGNNWTTV--------------VILIEPHHANSNYACESLSLNDT 385
VAASSVD SFLVAITLGNNWTT+ ++ E + ACESLSLNDT
Sbjct: 241 VAASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDT 300
Query: 386 WAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEI 445
WAAGNVVLCFASDD+ND+TH+TS SVKKVG LGLIVAKNPTK VEPFIN+FPCVQ+SL+I
Sbjct: 301 WAAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDI 360
Query: 446 GMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAI 505
GM ILNYIRSTRNPQV IG S TR+GQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAI
Sbjct: 361 GMQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAI 420
Query: 506 LAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDP 565
LAAVPPSDPKD+NSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDP
Sbjct: 421 LAAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDP 480
Query: 566 YGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISG 625
YG IFAEG+P+ SA PFDFGGGIVNPNKAVDPGLVYDMGMADYI+YFCA GYN+SAISG
Sbjct: 481 YGAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISG 540
Query: 626 ITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGI 685
ITK+ ISCPKRRPSILD NVPSITIP L H V++TRTVTNVGA+NS+YKAAIE P PGI
Sbjct: 541 ITKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAP--PGI 600
Query: 686 AIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 730
IAVKPR+LKFNHK K ISFTVT+SSNHRVTTGYCFGSLTWLDGVHSVRIP+SVRT+I
Sbjct: 601 TIAVKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655
BLAST of MS009726 vs. ExPASy TrEMBL
Match:
A0A6J1J1Z1 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)
HSP 1 Score: 1060.1 bits (2740), Expect = 4.3e-306
Identity = 530/633 (83.73%), Postives = 571/633 (90.21%), Query Frame = 0
Query: 111 MGNGAIIGLLDTAGIWPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYF 170
MG+GAIIG+LDT GIWPESEVF D GLGPVPSRWKGICESGELFSP KACSRKLIGARYF
Sbjct: 1 MGSGAIIGVLDT-GIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYF 60
Query: 171 IKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAP 230
+KGLEAAYGHP NNS FQDYLSPRD +GHGTHVSSVASG FV NVSYHGLAVGTVRGGAP
Sbjct: 61 VKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAP 120
Query: 231 QSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAI 290
SR+AMYKVCWQ+NGGVC+D DILKAIDQAI+DGVDVLSLSLGP+FPSYSDVDM NG+AI
Sbjct: 121 HSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAI 180
Query: 291 GTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV-- 350
G FHA+ KGIVVVGAAGN GPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTT+
Sbjct: 181 GAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQ 240
Query: 351 ------------VILIEPHHANSNYACESLSLNDTWAAGNVVLCFASDDFNDNTHHTSRS 410
++ E + ACESLSLNDTWA+GNVVLCFASDD+NDNTH+TS S
Sbjct: 241 GMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWS 300
Query: 411 VKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRI 470
VKKVG LGLIVAKNPTK VEPFI++FPCVQ+SL+IGM ILNYIRSTRNPQV IG S TR+
Sbjct: 301 VKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRV 360
Query: 471 GQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMAT 530
GQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK++NSYAFISGTSMAT
Sbjct: 361 GQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMAT 420
Query: 531 PHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIV 590
PHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG IFAEGRP+ SA PFDFGGGIV
Sbjct: 421 PHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIV 480
Query: 591 NPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITI 650
NPNKAVDPGLVYDMGMADYI+YFCA GYN+SAISGI+K+ ISCPKRRPSILD NVPSITI
Sbjct: 481 NPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITI 540
Query: 651 PGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVS 710
P L HSV++TRTVTNVGA+NS+YKAAIE P PGI IAVKPR+LKFNHK K ISFTVT+S
Sbjct: 541 PSLTHSVSLTRTVTNVGAINSSYKAAIEVP--PGITIAVKPRILKFNHKTKSISFTVTIS 600
Query: 711 SNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 730
SN RVTTGYCFGSLTWLDGVHSVRIP+SVRT+I
Sbjct: 601 SNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 630
BLAST of MS009726 vs. TAIR 10
Match:
AT1G32960.1 (Subtilase family protein )
HSP 1 Score: 788.1 bits (2034), Expect = 6.0e-228
Identity = 408/744 (54.84%), Postives = 525/744 (70.56%), Query Frame = 0
Query: 7 QVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQA 66
+V++VY+G++ H + E + ++HH +LAS+LGS++ DSMVY+Y++GFSGFAAKLT +QA
Sbjct: 31 KVHIVYLGEKKHHDPEFVTESHHQMLASLLGSKKDADDSMVYSYRHGFSGFAAKLTKSQA 90
Query: 67 QKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIW 126
+KI++LP V+ VIP+ H++ TTR+W+YL LS +LL + MG+ IIG++DT G+W
Sbjct: 91 KKIADLPEVVHVIPDGFHELATTRTWEYLGLSSANPKNLLNDTNMGDQVIIGVIDT-GVW 150
Query: 127 PESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSG 186
PESE F+DNG+GP+P +WKG CESGE F C+RKLIGA+YFI G A +N +
Sbjct: 151 PESESFNDNGVGPIPRKWKGGCESGENFR-STDCNRKLIGAKYFINGF-LAENKGFNTTE 210
Query: 187 FQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCW---QL 246
+DY+S RD+ GHGTHV+S+A GSFV NVSY GLA GT+RGGAP++R+AMYK CW +L
Sbjct: 211 SRDYISARDFDGHGTHVASIAGGSFVPNVSYKGLAGGTLRGGAPRARIAMYKACWFHEEL 270
Query: 247 NGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVV 306
G CSD+DI+KAID+AIHDGVDVLS+SL P S+ D+ + A G FHA+ KGIVVV
Sbjct: 271 KGVTCSDSDIMKAIDEAIHDGVDVLSISLVGQIPLNSETDIRDEFATGLFHAVAKGIVVV 330
Query: 307 GAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTT------------VVIL 366
A GN GPAA +V NI PW+LTVAA+++DRSF ITLGNN + L
Sbjct: 331 CAGGNDGPAAQTVVNIAPWILTVAATTLDRSFPTPITLGNNKVILGQATYTGPELGLTSL 390
Query: 367 IEPHHANSNY-----ACESLSLNDTWA-AGNVVLCFASDDFNDNTHHTSRSVKKVGALGL 426
+ P +A +N CESL+LN + A VVLCF + N + VK G LGL
Sbjct: 391 VYPENARNNNETFSGVCESLNLNPNYTMAMKVVLCFTASRTNAAISRAASFVKAAGGLGL 450
Query: 427 IVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVA 486
I+++NP + P + FPCV V E+G IL+YIRSTR+P V I SRT GQ + T V
Sbjct: 451 IISRNPVYTLSPCNDDFPCVAVDYELGTDILSYIRSTRSPVVKIQRSRTLSGQPVGTKVV 510
Query: 487 YFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVAL 546
FSSRGPNS++PAILKPDIAAPGV ILAA P+D + +A +SGTSMATP +S ++AL
Sbjct: 511 NFSSRGPNSMSPAILKPDIAAPGVRILAATSPNDTLNVGGFAMLSGTSMATPVISGVIAL 570
Query: 547 LKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPG 606
LK+LH WSPAA +SAIVTTAW +DP+GE IFAEG K + PFD+GGGIVNP KA +PG
Sbjct: 571 LKALHPEWSPAAFRSAIVTTAWRTDPFGEQIFAEGSSRKVSDPFDYGGGIVNPEKAAEPG 630
Query: 607 LVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTV 666
L+YDMG DYI Y C+ GYNDS+IS + + C +PS+LD N+PSITIP LK VT+
Sbjct: 631 LIYDMGPQDYILYLCSAGYNDSSISQLVGQITVCSNPKPSVLDVNLPSITIPNLKDEVTL 690
Query: 667 TRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGY 726
TRTVTNVG V+S YK ++E P G+ + V P L FN K +SFTV VS+ H++ TGY
Sbjct: 691 TRTVTNVGLVDSVYKVSVEPP--LGVRVVVTPETLVFNSKTISVSFTVRVSTTHKINTGY 750
Query: 727 CFGSLTWLDGVHSVRIPISVRTDI 730
FGSLTW D VH+V IP+SVRT I
Sbjct: 751 YFGSLTWTDSVHNVVIPLSVRTQI 769
BLAST of MS009726 vs. TAIR 10
Match:
AT4G10520.1 (Subtilase family protein )
HSP 1 Score: 785.8 bits (2028), Expect = 3.0e-227
Identity = 415/742 (55.93%), Postives = 528/742 (71.16%), Query Frame = 0
Query: 1 FLLGAQQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAK 60
F++ +VYVVY+G++ HDN E + ++HH +L S+LGS+EA DS+VY+Y++GFSGFAAK
Sbjct: 22 FVVAESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAK 81
Query: 61 LTMAQAQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLL 120
LT +QAQ+ISELP V++VIPN L++M TTR+WDYL +SP +SLLQK+ MG I+G++
Sbjct: 82 LTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVSPGNSDSLLQKANMGYNVIVGVI 141
Query: 121 DTAGIWPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGH 180
D +G+WPESE+F+D G GP+PSRWKG CESGELF+ C+RKLIGA+YF+ GL A +G
Sbjct: 142 D-SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFG- 201
Query: 181 PYNNSGFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVC 240
N + +YLSPRD+AGHGTHV+S GSF+ NVSY GL GT RGGAP +A+YK C
Sbjct: 202 VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKAC 261
Query: 241 WQLNGGVCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGI 300
W G CS AD+LKA+D+AIHDGVD+LSLSLGP+ P + + + ++G FHA+ KGI
Sbjct: 262 W---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETE---HTSVGAFHAVAKGI 321
Query: 301 VVVGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTV------------ 360
VV AAGN GP A +++N+ PW+LTVAA++ DRSF AITLGNN T +
Sbjct: 322 PVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAITLGNNITILGQAIYGGPELGF 381
Query: 361 VILIEPHHANSNYACESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIV 420
V L P S CE LS N ++ G VVLCFA+ + ++ +V G LGLI+
Sbjct: 382 VGLTYPESPLSG-DCEKLSANPNSTMEGKVVLCFAA---STPSNAAIAAVINAGGLGLIM 441
Query: 421 AKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYF 480
AKNPT + P FP V + E+G IL YIRSTR+P V I S+T GQS+ST VA F
Sbjct: 442 AKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATF 501
Query: 481 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLK 540
SSRGPNSV+PAILKPDIAAPGV ILAA+ P+ + +A +SGTSMATP VS +V LLK
Sbjct: 502 SSRGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLK 561
Query: 541 SLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLV 600
SLH WSP+AIKSAIVTTAW +DP GE IFA+G K A PFD+GGG++NP KAV PGL+
Sbjct: 562 SLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLI 621
Query: 601 YDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTR 660
YDM DY+ Y C++ Y+D +IS + K CP +PS+LD N+PSITIP L+ VT+TR
Sbjct: 622 YDMTTDDYVMYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTR 681
Query: 661 TVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCF 720
TVTNVG VNS YK I+ P GI +AV P L F++ SFTV VS+ H+V TGY F
Sbjct: 682 TVTNVGPVNSVYKVVIDPP--TGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYF 741
Query: 721 GSLTWLDGVHSVRIPISVRTDI 730
GSLTW D +H+V IP+SVRT I
Sbjct: 742 GSLTWTDNMHNVAIPVSVRTQI 748
BLAST of MS009726 vs. TAIR 10
Match:
AT4G10550.1 (Subtilase family protein )
HSP 1 Score: 774.2 bits (1998), Expect = 9.0e-224
Identity = 391/745 (52.48%), Postives = 526/745 (70.60%), Query Frame = 0
Query: 6 QQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQ 65
++V++VY+G++ HD+ E + ++HH +L S+LGS+E DSMVY+Y++GFSGFAAKLT +Q
Sbjct: 32 RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQ 91
Query: 66 AQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGI 125
A+KI++LP V+ VIP+ +K+ TTR+WDYL LS SLL ++ MG IIG++DT G+
Sbjct: 92 AKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT-GV 151
Query: 126 WPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNS 185
WPESEVF+D+G GPVPS WKG CE+GE F+ C++KLIGA+YFI G A +N++
Sbjct: 152 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFNST 211
Query: 186 GFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNG 245
D++SPRD GHGTHVS++A GSFV N+SY GLA GTVRGGAP++ +AMYK CW L+
Sbjct: 212 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 271
Query: 246 G---VCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVV 305
CS ADILKA+D+A+HDGVDVLS+SLG + P Y + D+ +G+ G FHA++KGI V
Sbjct: 272 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 331
Query: 306 VGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNN---------------WTT 365
V + GN GP + +V N PW++TVAA+++DRSF +TLGNN +T+
Sbjct: 332 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTS 391
Query: 366 VVILIEPHHANSNYA--CESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALG 425
+V P ++N +++ CE L N + G VVLCF + + +R VK+ G LG
Sbjct: 392 LVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGGLG 451
Query: 426 LIVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTV 485
+I+A++P ++P ++ FPCV V E+G IL Y RS+ +P V I PS+T +GQ + T V
Sbjct: 452 VIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKV 511
Query: 486 AYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVA 545
A FSSRGPNS+APAILKPDIAAPGV+ILAA + D+ + +SGTSMA P +S + A
Sbjct: 512 ATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGVAA 571
Query: 546 LLKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDP 605
LLK+LH WSPAAI+SAIVTTAW +DP+GE IFAEG P K A PFD+GGG+VNP K+ +P
Sbjct: 572 LLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANP 631
Query: 606 GLVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVT 665
GLVYDMG+ DY+ Y C++GYN+++IS + K C +PS+LD N+PSITIP LK VT
Sbjct: 632 GLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVT 691
Query: 666 VTRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTG 725
+TRTVTNVG +NS Y+ +E P G + V P L FN K + F V VS+ H+ TG
Sbjct: 692 ITRTVTNVGPLNSVYRVTVEPP--LGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 751
Query: 726 YCFGSLTWLDGVHSVRIPISVRTDI 730
Y FGSLTW D +H+V IP+SVRT I
Sbjct: 752 YYFGSLTWSDSLHNVTIPLSVRTQI 770
BLAST of MS009726 vs. TAIR 10
Match:
AT4G10550.3 (Subtilase family protein )
HSP 1 Score: 774.2 bits (1998), Expect = 9.0e-224
Identity = 391/745 (52.48%), Postives = 526/745 (70.60%), Query Frame = 0
Query: 6 QQVYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQ 65
++V++VY+G++ HD+ E + ++HH +L S+LGS+E DSMVY+Y++GFSGFAAKLT +Q
Sbjct: 48 RKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAAKLTESQ 107
Query: 66 AQKISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGI 125
A+KI++LP V+ VIP+ +K+ TTR+WDYL LS SLL ++ MG IIG++DT G+
Sbjct: 108 AKKIADLPDVVHVIPDSFYKLATTRTWDYLGLSAANPKSLLHETNMGEQIIIGVIDT-GV 167
Query: 126 WPESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNS 185
WPESEVF+D+G GPVPS WKG CE+GE F+ C++KLIGA+YFI G A +N++
Sbjct: 168 WPESEVFNDSGFGPVPSHWKGGCETGENFNSSN-CNKKLIGAKYFINGF-LAENESFNST 227
Query: 186 GFQDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNG 245
D++SPRD GHGTHVS++A GSFV N+SY GLA GTVRGGAP++ +AMYK CW L+
Sbjct: 228 NSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKACWYLDD 287
Query: 246 G---VCSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVV 305
CS ADILKA+D+A+HDGVDVLS+SLG + P Y + D+ +G+ G FHA++KGI V
Sbjct: 288 DDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAVLKGITV 347
Query: 306 VGAAGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNN---------------WTT 365
V + GN GP + +V N PW++TVAA+++DRSF +TLGNN +T+
Sbjct: 348 VCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPLTLGNNKVILGQAMYTGPGLGFTS 407
Query: 366 VVILIEPHHANSNYA--CESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALG 425
+V P ++N +++ CE L N + G VVLCF + + +R VK+ G LG
Sbjct: 408 LVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYVKRAGGLG 467
Query: 426 LIVAKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTV 485
+I+A++P ++P ++ FPCV V E+G IL Y RS+ +P V I PS+T +GQ + T V
Sbjct: 468 VIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVGQPVGTKV 527
Query: 486 AYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVA 545
A FSSRGPNS+APAILKPDIAAPGV+ILAA + D+ + +SGTSMA P +S + A
Sbjct: 528 ATFSSRGPNSIAPAILKPDIAAPGVSILAATTNTTFSDQ-GFIMLSGTSMAAPAISGVAA 587
Query: 546 LLKSLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDP 605
LLK+LH WSPAAI+SAIVTTAW +DP+GE IFAEG P K A PFD+GGG+VNP K+ +P
Sbjct: 588 LLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVNPEKSANP 647
Query: 606 GLVYDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVT 665
GLVYDMG+ DY+ Y C++GYN+++IS + K C +PS+LD N+PSITIP LK VT
Sbjct: 648 GLVYDMGLEDYVLYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITIPNLKDEVT 707
Query: 666 VTRTVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTG 725
+TRTVTNVG +NS Y+ +E P G + V P L FN K + F V VS+ H+ TG
Sbjct: 708 ITRTVTNVGPLNSVYRVTVEPP--LGFQVTVTPETLVFNSTTKKVYFKVKVSTTHKTNTG 767
Query: 726 YCFGSLTWLDGVHSVRIPISVRTDI 730
Y FGSLTW D +H+V IP+SVRT I
Sbjct: 768 YYFGSLTWSDSLHNVTIPLSVRTQI 786
BLAST of MS009726 vs. TAIR 10
Match:
AT4G10510.1 (Subtilase family protein )
HSP 1 Score: 773.1 bits (1995), Expect = 2.0e-223
Identity = 396/742 (53.37%), Postives = 529/742 (71.29%), Query Frame = 0
Query: 8 VYVVYMGKRPHDNEELLVKTHHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQ 67
V++VY+G++ HD+ E + ++HH +L S+LGS+E SMV+++++GFSGFAAKLT +QA+
Sbjct: 22 VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81
Query: 68 KISELPTVIKVIPNRLHKMQTTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTAGIWP 127
KI++LP V+ VIP+R +K TTR+WDYL LSP +LL ++ MG IIG++D +G+WP
Sbjct: 82 KIADLPEVVHVIPDRFYKPATTRTWDYLGLSPTNPKNLLNQTNMGEQMIIGIID-SGVWP 141
Query: 128 ESEVFSDNGLGPVPSRWKGICESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGF 187
ESEVF+DN +GPVPS WKG CESGE F+ C++KLIGA+YFI A + +N+S
Sbjct: 142 ESEVFNDNEIGPVPSHWKGGCESGEDFN-SSHCNKKLIGAKYFINAFLATH-ESFNSSES 201
Query: 188 QDYLSPRDYAGHGTHVSSVASGSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGV 247
D++SPR Y GHGTHV+++A GS+V N SY GLA GTVRGGAP++R+A+YK CW L+ +
Sbjct: 202 LDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDI 261
Query: 248 --CSDADILKAIDQAIHDGVDVLSLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGA 307
CS ADILKA+D+AIHDGVDVLSLSLG P Y + D+ +G+A G FHA++KGI VV A
Sbjct: 262 AACSSADILKAMDEAIHDGVDVLSLSLG-FEPLYPETDVRDGIATGAFHAVLKGITVVCA 321
Query: 308 AGNYGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNN---------------WTTVVI 367
AGN GPAA +V N PW+LTVAA+++DRSF+ +TLGNN +T++V
Sbjct: 322 AGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPMTLGNNKVILGQAIYTGTEVGFTSLVY 381
Query: 368 LIEPHHANSNYA--CESLSLN-DTWAAGNVVLCFASDDFNDNTHHTSRSVKKVGALGLIV 427
P ++N +++ CE L +N + AG VVLCF ++ + + VK+ G LG+I+
Sbjct: 382 PENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVII 441
Query: 428 AKNPTKDVEPFINHFPCVQVSLEIGMHILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYF 487
A P + P ++ FPCV V E+G +IL YIRS +P V I PSRT IGQ + T VA F
Sbjct: 442 AGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVASF 501
Query: 488 SSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKDKNSYAFISGTSMATPHVSAIVALLK 547
SSRGPN ++ AILKPDIAAPGV+ILAA + + + F+SGTSMATP +S IVALLK
Sbjct: 502 SSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALLK 561
Query: 548 SLHSHWSPAAIKSAIVTTAWTSDPYGETIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLV 607
+LH WSPAAI+SAIVTTAW +DP+GE IFAEG P K A PFD+GGG+VNP KA PGLV
Sbjct: 562 ALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGLV 621
Query: 608 YDMGMADYIRYFCAMGYNDSAISGITKKPISCPKRRPSILDTNVPSITIPGLKHSVTVTR 667
YD+G+ DY+ Y C++GYN+++IS + K C +PS+LD N+PSITIP LK VT+ R
Sbjct: 622 YDLGLEDYVLYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTLPR 681
Query: 668 TVTNVGAVNSTYKAAIETPPGPGIAIAVKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCF 727
T+TNVG + S Y+ A+E P G + V P L FN K +SF V+VS+ H++ TGY F
Sbjct: 682 TLTNVGPLESVYRVAVEPP--LGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYF 741
Query: 728 GSLTWLDGVHSVRIPISVRTDI 730
GSLTW D +H+V IP+SVRT +
Sbjct: 742 GSLTWSDSLHNVTIPLSVRTQL 757
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022141072.1 | 0.0e+00 | 96.33 | subtilisin-like protease SBT3.9 [Momordica charantia] | [more] |
| KAG7014677.1 | 0.0e+00 | 83.97 | Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma] | [more] |
| KAG6576624.1 | 0.0e+00 | 83.70 | Subtilisin-like protease 3.3, partial [Cucurbita argyrosperma subsp. sororia] | [more] |
| XP_022922598.1 | 0.0e+00 | 83.83 | subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata] | [more] |
| XP_023551712.1 | 0.0e+00 | 83.70 | subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9MAP5 | 8.5e-227 | 54.84 | Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 S... | [more] |
| Q9ZSB0 | 4.2e-226 | 55.93 | Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 S... | [more] |
| Q9ZSB1 | 3.9e-224 | 56.06 | Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana OX=3702 GN=SBT3.10 PE=3... | [more] |
| Q8L7I2 | 1.3e-222 | 52.48 | Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S... | [more] |
| Q9SZY2 | 2.2e-222 | 53.30 | Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CIU2 | 0.0e+00 | 96.33 | subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 P... | [more] |
| A0A6J1E757 | 0.0e+00 | 83.83 | subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1J508 | 0.0e+00 | 83.70 | subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
| A0A6J1E3V3 | 0.0e+00 | 83.89 | subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC1... | [more] |
| A0A6J1J1Z1 | 4.3e-306 | 83.73 | subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
