MS009716 (gene) Bitter gourd (TR) v1

BLAST of MS009716 vs. NCBI nr
Match: XP_022141208.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Momordica charantia])

HSP 1 Score: 934.9 bits (2415), Expect = 2.7e-268
Identity = 451/453 (99.56%), Postives = 452/453 (99.78%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456

BLAST of MS009716 vs. NCBI nr
Match: XP_022141209.1 (uncharacterized calcium-binding protein At1g02270 isoform X2 [Momordica charantia])

HSP 1 Score: 928.3 bits (2398), Expect = 2.5e-266
Identity = 450/453 (99.34%), Postives = 451/453 (99.56%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEF QRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEF-QRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 455

BLAST of MS009716 vs. NCBI nr
Match: XP_022141210.1 (uncharacterized calcium-binding protein At1g02270 isoform X3 [Momordica charantia])

HSP 1 Score: 891.3 bits (2302), Expect = 3.4e-255
Identity = 434/453 (95.81%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMF 
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMF- 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
                            KADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 -----------------KADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 438

BLAST of MS009716 vs. NCBI nr
Match: XP_022923111.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita moschata])

HSP 1 Score: 890.6 bits (2300), Expect = 5.8e-255
Identity = 432/453 (95.36%), Postives = 439/453 (96.91%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRL NAGY+SFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLRNAGYISFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
            DGNGILD+NEFQQRIWNSTG EERD + NEV    ESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 SDGNGILDHNEFQQRIWNSTGTEERDEKGNEV----ESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MS009716 vs. NCBI nr
Match: XP_022984360.1 (uncharacterized calcium-binding protein At1g02270 isoform X1 [Cucurbita maxima] >KAG7014667.1 putative calcium-binding protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 890.2 bits (2299), Expect = 7.5e-255
Identity = 432/453 (95.36%), Postives = 440/453 (97.13%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRL+QQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLSQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRL NAGY+SFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLRNAGYISFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
            DGNGILD+NEFQQRIWNSTG EERD +SNEV    ESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 SDGNGILDHNEFQQRIWNSTGTEERDEKSNEV----ESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MS009716 vs. ExPASy Swiss-Prot
Match: O81916 (Uncharacterized calcium-binding protein At1g02270 OS=Arabidopsis thaliana OX=3702 GN=At1g02270 PE=2 SV=2)

HSP 1 Score: 592.0 bits (1525), Expect = 5.6e-168
Identity = 278/417 (66.67%), Postives = 343/417 (82.25%), Query Frame = 0

Query: 33  ITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLARNQRILDWLLYEKSSIICLQQEFWVG 92
           I+CTTFNILAPIYKR++Q++ S RESD+RT WLARNQRILD LL+++SS+ICL QE WVG
Sbjct: 71  ISCTTFNILAPIYKRVDQKNHSTRESDFRTLWLARNQRILDLLLHQRSSVICL-QEVWVG 130

Query: 93  NEELVNIYENRLANAGYLSFKLARTNNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQ 152
           NEELVN+Y ++L+++GY  ++LARTN+RGDGLLTA++KD+FKVVNYRELLFNDFGDRVAQ
Sbjct: 131 NEELVNMYHHQLSSSGYTIYQLARTNSRGDGLLTAIHKDHFKVVNYRELLFNDFGDRVAQ 190

Query: 153 LLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENK 212
           LLHV+  +PF      D++QE++IVNTHLLFPHDSSL +VRL+QVYKIL+Y+E+YQKENK
Sbjct: 191 LLHVKTVIPFPLNGKQDVQQEVIIVNTHLLFPHDSSLSIVRLHQVYKILEYLEAYQKENK 250

Query: 213 LNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGV 272
           LN MPIILCGDWNGSKRGHVYKFLRSQGF+SSYD AHQYTD+DA +WVSHRNHRGNICGV
Sbjct: 251 LNHMPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDSDAHRWVSHRNHRGNICGV 310

Query: 273 DFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFC 332
           DFIWL NP+  RK L+TSW EAVF + KY L +AS+ EDDAF FL A N  D +TYS FC
Sbjct: 311 DFIWLCNPSDSRKPLRTSWVEAVFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFC 370

Query: 333 EALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEV 392
            AL+++NLTG PHGLS  E ++LWV+AD+DGNG+ DY E  ++IWN T    + G   E 
Sbjct: 371 LALQKVNLTGIPHGLSFEETKELWVRADLDGNGVFDYEEL-KKIWNMT-MVNQPGNCKES 430

Query: 393 GSGGESKENQEQTIGFSVENAVLFPAEVEKGRWPEDYSLSDHARLTVVFAPIRMPCS 450
               + +E +++ IG  V  A+LFP E EKG WPE+Y++SDHA LTV F+P++M CS
Sbjct: 431 VMESKKEEGEDEAIGLKVNKAILFPQEAEKGLWPENYNISDHACLTVQFSPVKMLCS 484

BLAST of MS009716 vs. ExPASy Swiss-Prot
Match: Q9LS39 (Carbon catabolite repressor protein 4 homolog 3 OS=Arabidopsis thaliana OX=3702 GN=CCR4-3 PE=2 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 2.6e-08
Identity = 50/178 (28.09%), Postives = 86/178 (48.31%), Query Frame = 0

Query: 64  WLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDG 123
           W  R + I + L+     II +Q+      ++  +++ + +  AGY      RT +  DG
Sbjct: 139 WGYRKRLICEELIRLNPDIISMQEV-----DKYFDLF-SMMEKAGYAGSYKRRTGDNVDG 198

Query: 124 LLTAVNKDYFKVVNYRELLFNDFG--DRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHL 183
                  D F V+    + F+ FG  D VAQL  +EL       R ++  ++IL+ N H+
Sbjct: 199 CAMFWKADRFGVLERENIEFSQFGMRDNVAQLAVLEL-------RKSNKSRKILLGNIHV 258

Query: 184 LF-PHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRS 239
           L+ P+      V+L QV  +    +++    K   +PI+LCGD+N + +  +Y FL S
Sbjct: 259 LYNPNQGD---VKLGQVRSLCS--KAHLLSKKWGDIPIVLCGDFNSTPKSPLYNFLAS 298

BLAST of MS009716 vs. ExPASy TrEMBL
Match: A0A6J1CJT7 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.3e-268
Identity = 451/453 (99.56%), Postives = 452/453 (99.78%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 456

BLAST of MS009716 vs. ExPASy TrEMBL
Match: A0A6J1CHY0 (uncharacterized calcium-binding protein At1g02270 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 928.3 bits (2398), Expect = 1.2e-266
Identity = 450/453 (99.34%), Postives = 451/453 (99.56%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFL+ADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLQADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEF QRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEF-QRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 455

BLAST of MS009716 vs. ExPASy TrEMBL
Match: A0A6J1CHC3 (uncharacterized calcium-binding protein At1g02270 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111011664 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.6e-255
Identity = 434/453 (95.81%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMF 
Sbjct: 245 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMF- 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
                            KADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD
Sbjct: 305 -----------------KADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
           IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 438

BLAST of MS009716 vs. ExPASy TrEMBL
Match: A0A6J1E5X5 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430875 PE=4 SV=1)

HSP 1 Score: 890.6 bits (2300), Expect = 2.8e-255
Identity = 432/453 (95.36%), Postives = 439/453 (96.91%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRLNQQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLNQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRL NAGY+SFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLRNAGYISFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
            DGNGILD+NEFQQRIWNSTG EERD + NEV    ESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 SDGNGILDHNEFQQRIWNSTGTEERDEKGNEV----ESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MS009716 vs. ExPASy TrEMBL
Match: A0A6J1J8E9 (uncharacterized calcium-binding protein At1g02270 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482685 PE=4 SV=1)

HSP 1 Score: 890.2 bits (2299), Expect = 3.6e-255
Identity = 432/453 (95.36%), Postives = 440/453 (97.13%), Query Frame = 0

Query: 1   RRRDKKMKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDY 60
           RRRDKKMKGRISMIGSYAIASSIKD HHQRP ITCTTFNILAPIYKRL+QQDPSCRESDY
Sbjct: 5   RRRDKKMKGRISMIGSYAIASSIKDHHHQRPSITCTTFNILAPIYKRLSQQDPSCRESDY 64

Query: 61  RTYWLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNR 120
           RTYWLARNQRILDWLLYEKSSIICL QEFWVGNEELVNIYENRL NAGY+SFKLARTNNR
Sbjct: 65  RTYWLARNQRILDWLLYEKSSIICL-QEFWVGNEELVNIYENRLRNAGYISFKLARTNNR 124

Query: 121 GDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 180
           GDGLLTAV+KDYF+VVNYRELLF+D GDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH
Sbjct: 125 GDGLLTAVHKDYFRVVNYRELLFHDCGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTH 184

Query: 181 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 240
           LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG
Sbjct: 185 LLFPHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQG 244

Query: 241 FVSSYDTAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 300
           FVSSYDTAHQY DADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK
Sbjct: 245 FVSSYDTAHQYADADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFK 304

Query: 301 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQAD 360
           YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEI+DLWVQAD
Sbjct: 305 YLLRRASLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIKDLWVQAD 364

Query: 361 IDGNGILDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEV 420
            DGNGILD+NEFQQRIWNSTG EERD +SNEV    ESKENQEQTIGFSVENAVLFPAEV
Sbjct: 365 SDGNGILDHNEFQQRIWNSTGTEERDEKSNEV----ESKENQEQTIGFSVENAVLFPAEV 424

Query: 421 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 454
           EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS
Sbjct: 425 EKGRWPEDYSLSDHARLTVVFAPIRMPCSQLIS 452

BLAST of MS009716 vs. TAIR 10
Match: AT5G54130.2 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 739.6 bits (1908), Expect = 1.5e-213
Identity = 357/447 (79.87%), Postives = 394/447 (88.14%), Query Frame = 0

Query: 7   MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 66
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 67  RNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLT 126
           RN RI+DWLLYE+SSIICL QEFWVGNEELVN+YE RL +AGYLS+KL RTNNRGDGLLT
Sbjct: 61  RNHRIIDWLLYERSSIICL-QEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNRGDGLLT 120

Query: 127 AVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHD 186
           AV+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHD
Sbjct: 121 AVHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHD 180

Query: 187 SSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 246
           S+L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD
Sbjct: 181 STLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 240

Query: 247 TAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRA 306
           TAH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRA
Sbjct: 241 TAHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRA 300

Query: 307 SLTEDDAFAFLKADNDGDYITYSGFCEALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGI 366
           SLT +DAFAFLK DNDGD+IT+ GFCE LRQLNLTGH +GL+  EI+DLW QADIDGNG+
Sbjct: 301 SLTAEDAFAFLKTDNDGDHITFMGFCETLRQLNLTGHCNGLTTKEIKDLWTQADIDGNGL 360

Query: 367 LDYNEFQQRIWNSTGNEERDGRSNEVGSGGESKENQEQTIGFSVENAVLFPAEVEKGRWP 426
           LDY EFQQRIWN T +E+RD         GE+K NQEQT+GFSV+NAVLFP EVEKG WP
Sbjct: 361 LDYKEFQQRIWNQTWSEQRDAED------GEAKGNQEQTVGFSVKNAVLFPPEVEKGMWP 420

Query: 427 EDYSLSDHARLTVVFAPIRMPCSQLIS 454
           E+YSLSDHARLTVVF+PIRMPCSQL+S
Sbjct: 421 ENYSLSDHARLTVVFSPIRMPCSQLVS 436

BLAST of MS009716 vs. TAIR 10
Match: AT1G02270.1 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 592.0 bits (1525), Expect = 3.9e-169
Identity = 278/417 (66.67%), Postives = 343/417 (82.25%), Query Frame = 0

Query: 33  ITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLARNQRILDWLLYEKSSIICLQQEFWVG 92
           I+CTTFNILAPIYKR++Q++ S RESD+RT WLARNQRILD LL+++SS+ICL QE WVG
Sbjct: 71  ISCTTFNILAPIYKRVDQKNHSTRESDFRTLWLARNQRILDLLLHQRSSVICL-QEVWVG 130

Query: 93  NEELVNIYENRLANAGYLSFKLARTNNRGDGLLTAVNKDYFKVVNYRELLFNDFGDRVAQ 152
           NEELVN+Y ++L+++GY  ++LARTN+RGDGLLTA++KD+FKVVNYRELLFNDFGDRVAQ
Sbjct: 131 NEELVNMYHHQLSSSGYTIYQLARTNSRGDGLLTAIHKDHFKVVNYRELLFNDFGDRVAQ 190

Query: 153 LLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHDSSLCLVRLNQVYKILQYVESYQKENK 212
           LLHV+  +PF      D++QE++IVNTHLLFPHDSSL +VRL+QVYKIL+Y+E+YQKENK
Sbjct: 191 LLHVKTVIPFPLNGKQDVQQEVIIVNTHLLFPHDSSLSIVRLHQVYKILEYLEAYQKENK 250

Query: 213 LNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYDTAHQYTDADARKWVSHRNHRGNICGV 272
           LN MPIILCGDWNGSKRGHVYKFLRSQGF+SSYD AHQYTD+DA +WVSHRNHRGNICGV
Sbjct: 251 LNHMPIILCGDWNGSKRGHVYKFLRSQGFISSYDDAHQYTDSDAHRWVSHRNHRGNICGV 310

Query: 273 DFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRASLTEDDAFAFLKADNDGDYITYSGFC 332
           DFIWL NP+  RK L+TSW EAVF + KY L +AS+ EDDAF FL A N  D +TYS FC
Sbjct: 311 DFIWLCNPSDSRKPLRTSWVEAVFSIIKYQLHKASIAEDDAFTFLGAKNHSDSLTYSDFC 370

Query: 333 EALRQLNLTGHPHGLSVGEIEDLWVQADIDGNGILDYNEFQQRIWNSTGNEERDGRSNEV 392
            AL+++NLTG PHGLS  E ++LWV+AD+DGNG+ DY E  ++IWN T    + G   E 
Sbjct: 371 LALQKVNLTGIPHGLSFEETKELWVRADLDGNGVFDYEEL-KKIWNMT-MVNQPGNCKES 430

Query: 393 GSGGESKENQEQTIGFSVENAVLFPAEVEKGRWPEDYSLSDHARLTVVFAPIRMPCS 450
               + +E +++ IG  V  A+LFP E EKG WPE+Y++SDHA LTV F+P++M CS
Sbjct: 431 VMESKKEEGEDEAIGLKVNKAILFPQEAEKGLWPENYNISDHACLTVQFSPVKMLCS 484

BLAST of MS009716 vs. TAIR 10
Match: AT5G54130.4 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 567.4 bits (1461), Expect = 1.0e-161
Identity = 272/334 (81.44%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 7   MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 66
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 67  RNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLT 126
           RN RI+DWLLYE+SSIICL QEFWVGNEELVN+YE RL +AGYLS+KL RTNNRGDGLLT
Sbjct: 61  RNHRIIDWLLYERSSIICL-QEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNRGDGLLT 120

Query: 127 AVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHD 186
           AV+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHD
Sbjct: 121 AVHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHD 180

Query: 187 SSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 246
           S+L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD
Sbjct: 181 STLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 240

Query: 247 TAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRA 306
           TAH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRA
Sbjct: 241 TAHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRA 300

Query: 307 SLTEDDAFAFLKADNDGDYITYSGFCEALRQLNL 341
           SLT +DAFAFLK DNDGD+IT+ GFCE LRQ+ +
Sbjct: 301 SLTAEDAFAFLKTDNDGDHITFMGFCETLRQVTV 329

BLAST of MS009716 vs. TAIR 10
Match: AT5G54130.3 (Calcium-binding endonuclease/exonuclease/phosphatase family )

HSP 1 Score: 557.8 bits (1436), Expect = 8.2e-159
Identity = 270/334 (80.84%), Postives = 297/334 (88.92%), Query Frame = 0

Query: 7   MKGRISMIGSYAIASSIKDQHHQRPCITCTTFNILAPIYKRLNQQDPSCRESDYRTYWLA 66
           MKGRIS IGSYAI+SSI+DQ HQ+PCI+CTTFNILAPIYKRL+ +D S RESD R YWL 
Sbjct: 1   MKGRISKIGSYAISSSIRDQ-HQQPCISCTTFNILAPIYKRLSHKDQSLRESDNRAYWLG 60

Query: 67  RNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDGLLT 126
           RN RI+DWLLYE+SSIICL QEFWVGNEELVN+YE RL +AGYLS+KL RTNNR  GLLT
Sbjct: 61  RNHRIIDWLLYERSSIICL-QEFWVGNEELVNLYEKRLGDAGYLSYKLGRTNNR--GLLT 120

Query: 127 AVNKDYFKVVNYRELLFNDFGDRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHLLFPHD 186
           AV+KDYF+VVN R+LLFND GDRVAQLLHVEL  P+SQ    D  QE+LIVNTHLLFPHD
Sbjct: 121 AVHKDYFRVVNSRDLLFNDCGDRVAQLLHVELVPPYSQ---YDAHQEVLIVNTHLLFPHD 180

Query: 187 SSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 246
           S+L +VRL QVYKILQYVESYQKE  L+PMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD
Sbjct: 181 STLSIVRLQQVYKILQYVESYQKEVNLSPMPIILCGDWNGSKRGHVYKFLRSQGFVSSYD 240

Query: 247 TAHQYTDADARKWVSHRNHRGNICGVDFIWLLNPNGYRKLLKTSWSEAVFGMFKYLLRRA 306
           TAH+YTD+DA KWVSHRNHRGNIC VDFIWLLNPN YRKLLKTSWSEAVFGMF+YLLRRA
Sbjct: 241 TAHRYTDSDAHKWVSHRNHRGNICAVDFIWLLNPNRYRKLLKTSWSEAVFGMFRYLLRRA 300

Query: 307 SLTEDDAFAFLKADNDGDYITYSGFCEALRQLNL 341
           SLT +DAFAFLK DNDGD+IT+ GFCE LRQ+ +
Sbjct: 301 SLTAEDAFAFLKTDNDGDHITFMGFCETLRQVTV 327

BLAST of MS009716 vs. TAIR 10
Match: AT3G18500.2 (DNAse I-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.9e-09
Identity = 50/178 (28.09%), Postives = 86/178 (48.31%), Query Frame = 0

Query: 64  WLARNQRILDWLLYEKSSIICLQQEFWVGNEELVNIYENRLANAGYLSFKLARTNNRGDG 123
           W  R + I + L+     II +Q+      ++  +++ + +  AGY      RT +  DG
Sbjct: 139 WGYRKRLICEELIRLNPDIISMQEV-----DKYFDLF-SMMEKAGYAGSYKRRTGDNVDG 198

Query: 124 LLTAVNKDYFKVVNYRELLFNDFG--DRVAQLLHVELAVPFSQCRNNDIRQEILIVNTHL 183
                  D F V+    + F+ FG  D VAQL  +EL       R ++  ++IL+ N H+
Sbjct: 199 CAMFWKADRFGVLERENIEFSQFGMRDNVAQLAVLEL-------RKSNKSRKILLGNIHV 258

Query: 184 LF-PHDSSLCLVRLNQVYKILQYVESYQKENKLNPMPIILCGDWNGSKRGHVYKFLRS 239
           L+ P+      V+L QV  +    +++    K   +PI+LCGD+N + +  +Y FL S
Sbjct: 259 LYNPNQGD---VKLGQVRSLCS--KAHLLSKKWGDIPIVLCGDFNSTPKSPLYNFLAS 298