MS009606 (gene) Bitter gourd (TR) v1

Overview
NameMS009606
Typegene
OrganismMomordica charantia cv. TR (Bitter gourd (TR) v1)
DescriptionNucleoside diphosphate kinase
Locationscaffold813: 2653180 .. 2654414 (+)
RNA-Seq ExpressionMS009606
SyntenyMS009606
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGCAAGTAATGGGAATATGGGAACGGAGAAAACTTTGGCTATGATAAAACCAGATGGATTGCGTGGTAACTACACCAATAGAATTAAAAAGGCCATTGTGGAGTCAGGTTTTAGAATTTTGAAGGAAATTATTGTTGAATTGGATGAAGATAGAGCAAGTCGCTTTTATGCTGAACATTCGTCGAGAAACTTCTTTTCTGGTCTCGTCAAATACATGACAAGGTATATGTTGAATCTTTGAATTGTCGTCAAGTTTTCTTAACAAATCATTTTTTGCCTGATTTTTAAACGTTCCATGGTACTGTTGTATCAATACTGATATTGTACCTCACCGTCTTTGTTATTGCATTTTTAAGCCTGAATTTGAACTGCTTTCATTATGCATTAATTTGCTAGTAAGTGGTTGTTGTTATCCTTCTCACCCATGGAACTTGTGCAGCAATCAGACCACCATCTGCCTGCACTGAAACTAAAACGTGAAAATTCTGAAATTGCAAGATTTACTAATTCATGCAATTACTATTTTTCTTACTTTCTTTTCAAGTAATTTCAGTTAAGATATTAGCATAGCAACACAGAATTTCACGACATGTATCCCAAGCTTACCAGATGTTAGTCAGTGTGCTTCTCCACCTTGAGCCGTACTCTAATAGTTTAACTTAGTTGAAATGTGGGTCTATGTATGGCAGGAGACATTAAAAAACAATCTAGTAGACTTATGATCTGACATTTTTTTTTCTAGCCTTATTATGTAATTAAACAAACATTAAATCTCTCTAGAATTGTGCGATCCTGTATTTCAGTTCTTGCATAATGGTATTTTTCCTCTGTCTAACGTTACTCATCAGTGGCCCAGTATGCATTATGGTTTTGGAGAAACAAAATGCAATCGCAGATTGGCGTGCTATGATTGGGCCAACAGATGCTACGAAAGCTAAGGCTACACATCCCAATAGGTACTATAAATTTTTATCTTCGTCACTATATATACTTTGCCTACTGAAGTTTGAGACATGGATTCAATACCGATGTCAATACAGTTGCTACCGGTTCATTTGTAGTTTTGGTTTTCACTGGAGTCCTTTGCTTGATTTCAGCATTAGGGCAATGTGTGGCTTGGATTCAGAGAAAAATTGTGTTCATGGTTCAGATTCCCTTCAATCTGCTCAAAGAGAGATCTCATTTTTCTTTGAAGAGGAGACTGGTAAAATTTATCCCCGCTATCTCAAATAT

mRNA sequence

TTTGCAAGTAATGGGAATATGGGAACGGAGAAAACTTTGGCTATGATAAAACCAGATGGATTGCGTGGTAACTACACCAATAGAATTAAAAAGGCCATTGTGGAGTCAGGTTTTAGAATTTTGAAGGAAATTATTGTTGAATTGGATGAAGATAGAGCAAGTCGCTTTTATGCTGAACATTCGTCGAGAAACTTCTTTTCTGGTCTCGTCAAATACATGACAAGTGGCCCAGTATGCATTATGGTTTTGGAGAAACAAAATGCAATCGCAGATTGGCGTGCTATGATTGGGCCAACAGATGCTACGAAAGCTAAGGCTACACATCCCAATAGCATTAGGGCAATGTGTGGCTTGGATTCAGAGAAAAATTGTGTTCATGGTTCAGATTCCCTTCAATCTGCTCAAAGAGAGATCTCATTTTTCTTTGAAGAGGAGACTGGTAAAATTTATCCCCGCTATCTCAAATAT

Coding sequence (CDS)

TTTGCAAGTAATGGGAATATGGGAACGGAGAAAACTTTGGCTATGATAAAACCAGATGGATTGCGTGGTAACTACACCAATAGAATTAAAAAGGCCATTGTGGAGTCAGGTTTTAGAATTTTGAAGGAAATTATTGTTGAATTGGATGAAGATAGAGCAAGTCGCTTTTATGCTGAACATTCGTCGAGAAACTTCTTTTCTGGTCTCGTCAAATACATGACAAGTGGCCCAGTATGCATTATGGTTTTGGAGAAACAAAATGCAATCGCAGATTGGCGTGCTATGATTGGGCCAACAGATGCTACGAAAGCTAAGGCTACACATCCCAATAGCATTAGGGCAATGTGTGGCTTGGATTCAGAGAAAAATTGTGTTCATGGTTCAGATTCCCTTCAATCTGCTCAAAGAGAGATCTCATTTTTCTTTGAAGAGGAGACTGGTAAAATTTATCCCCGCTATCTCAAATAT

Protein sequence

FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSDSLQSAQREISFFFEEETGKIYPRYLKY
Homology
BLAST of MS009606 vs. NCBI nr
Match: XP_022148787.1 (probable nucleoside diphosphate kinase 5 isoform X2 [Momordica charantia])

HSP 1 Score: 295.0 bits (754), Expect = 3.7e-76
Identity = 146/153 (95.42%), Postives = 150/153 (98.04%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNGNM TEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH
Sbjct: 27  FASNGNMVTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIYPRY 154
           EKNCVHGSDSLQSAQREISFFFEEETG++  ++
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEEETGEMVGKH 179

BLAST of MS009606 vs. NCBI nr
Match: XP_022148770.1 (probable nucleoside diphosphate kinase 5 isoform X1 [Momordica charantia] >XP_022148779.1 probable nucleoside diphosphate kinase 5 isoform X1 [Momordica charantia])

HSP 1 Score: 292.0 bits (746), Expect = 3.1e-75
Identity = 145/146 (99.32%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNGNM TEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH
Sbjct: 27  FASNGNMVTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEET 147
           EKNCVHGSDSLQSAQREISFFFEEET
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEEET 172

BLAST of MS009606 vs. NCBI nr
Match: XP_022953616.1 (probable nucleoside diphosphate kinase 5 isoform X3 [Cucurbita moschata])

HSP 1 Score: 271.9 bits (694), Expect = 3.3e-69
Identity = 135/153 (88.24%), Postives = 145/153 (94.77%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIYPRY 154
           EKNCVHGSDSLQSAQREISFFF EETG+I  ++
Sbjct: 147 EKNCVHGSDSLQSAQREISFFF-EETGEIVGKH 178

BLAST of MS009606 vs. NCBI nr
Match: XP_022953615.1 (probable nucleoside diphosphate kinase 5 isoform X2 [Cucurbita moschata])

HSP 1 Score: 270.8 bits (691), Expect = 7.4e-69
Identity = 133/150 (88.67%), Postives = 141/150 (94.00%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIY 151
           EKNCVHGSDSLQSAQREISFFFEE    +Y
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEETGVLLY 176

BLAST of MS009606 vs. NCBI nr
Match: XP_023547543.1 (probable nucleoside diphosphate kinase 5 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.8 bits (691), Expect = 7.4e-69
Identity = 134/153 (87.58%), Postives = 145/153 (94.77%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKA+ATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKARATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIYPRY 154
           EKNCVHGSDSLQSAQREISFFF EETG+I  ++
Sbjct: 147 EKNCVHGSDSLQSAQREISFFF-EETGEIVGKH 178

BLAST of MS009606 vs. ExPASy Swiss-Prot
Match: Q6NLG3 (Probable nucleoside diphosphate kinase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g17410 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 2.2e-47
Identity = 86/130 (66.15%), Postives = 109/130 (83.85%), Query Frame = 0

Query: 15  MIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGLVKYMT 74
           MIKPDG+ GNYT  IK  +VE+GF I+KE++ +LD++ AS FY EHSSR+FF  LV YMT
Sbjct: 1   MIKPDGVSGNYTEEIKTIVVEAGFNIVKEMLTQLDKETASAFYEEHSSRSFFPHLVTYMT 60

Query: 75  SGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSDSLQSA 134
           SGPV +MVLEK+NA++DWR +IGPTDA KAK +HP+SIRA+CG +S+KNCVHGSDS  SA
Sbjct: 61  SGPVLVMVLEKRNAVSDWRDLIGPTDAEKAKISHPHSIRALCGKNSQKNCVHGSDSTSSA 120

Query: 135 QREISFFFEE 145
           +REI FFF++
Sbjct: 121 EREIKFFFKD 130

BLAST of MS009606 vs. ExPASy Swiss-Prot
Match: A5FY40 (Nucleoside diphosphate kinase OS=Acidiphilium cryptum (strain JF-5) OX=349163 GN=ndk PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.1e-27
Identity = 66/136 (48.53%), Postives = 88/136 (64.71%), Query Frame = 0

Query: 7   MGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFF 66
           M TE+TL++IKPD  R N T RI     E+G RI+ +  + L +D+A  FY  H  R FF
Sbjct: 1   MATERTLSIIKPDATRRNLTGRINAKFEEAGLRIVAQKRIRLTKDQAEAFYGVHKERPFF 60

Query: 67  SGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVH 126
           +GLV +MTSGPV + VLE  +A+A  RA++G TD  KA+A    +IRA    D E N VH
Sbjct: 61  AGLVSFMTSGPVVVQVLEGDDAVARNRAIMGATDPRKAEA---GTIRAEFAEDIEANSVH 120

Query: 127 GSDSLQSAQREISFFF 143
           GSD+  +A +EI+FFF
Sbjct: 121 GSDAADTAAQEIAFFF 133

BLAST of MS009606 vs. ExPASy Swiss-Prot
Match: A6VMK7 (Nucleoside diphosphate kinase OS=Actinobacillus succinogenes (strain ATCC 55618 / DSM 22257 / 130Z) OX=339671 GN=ndk PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.6e-26
Identity = 65/146 (44.52%), Postives = 91/146 (62.33%), Query Frame = 0

Query: 7   MGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFF 66
           M  E+TL++IKPD +  N   +I     E+GF+I+   ++ L++ +A  FYAEH  + FF
Sbjct: 1   MALERTLSIIKPDAVERNLVGKILSRFEENGFQIVAMKMLRLNQAQAEGFYAEHQGKPFF 60

Query: 67  SGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVH 126
            GLV+YMTS PV + VLEK NA+ D+R +IG TD  +A      +IR        +N VH
Sbjct: 61  DGLVEYMTSAPVVVSVLEKDNAVKDYRTLIGATDPQQAA---EGTIRKDFAESRRRNSVH 120

Query: 127 GSDSLQSAQREISFFFEEETGKIYPR 153
           GSDS +SA REI++FF E   +I PR
Sbjct: 121 GSDSEESAVREIAYFFVE--SEICPR 141

BLAST of MS009606 vs. ExPASy Swiss-Prot
Match: B0USF1 (Nucleoside diphosphate kinase OS=Histophilus somni (strain 2336) OX=228400 GN=ndk PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.6e-26
Identity = 61/146 (41.78%), Postives = 97/146 (66.44%), Query Frame = 0

Query: 7   MGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFF 66
           M  E+TLA+IKPD +  N    I   + + GF+I+   ++ L++++A  FYAEH ++ FF
Sbjct: 1   MTLEQTLAIIKPDAVERNLIGNIISRLEDKGFQIIAMKMLHLNQEQAEGFYAEHRNKAFF 60

Query: 67  SGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVH 126
           + LVKYMTS P+ ++VL+K+NA+ D+R  +G T+   A+     ++R    ++  +N VH
Sbjct: 61  TELVKYMTSAPIVVLVLQKENAVKDYRTFMGTTNPEIAE---NGTLRYEFAINQTQNSVH 120

Query: 127 GSDSLQSAQREISFFFEEETGKIYPR 153
           GSDSL++AQREI++FF E   +IY R
Sbjct: 121 GSDSLENAQREIAYFFAE--AEIYAR 141

BLAST of MS009606 vs. ExPASy Swiss-Prot
Match: Q1QD23 (Nucleoside diphosphate kinase OS=Psychrobacter cryohalolentis (strain ATCC BAA-1226 / DSM 17306 / VKM B-2378 / K5) OX=335284 GN=ndk PE=3 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 1.6e-26
Identity = 64/139 (46.04%), Postives = 89/139 (64.03%), Query Frame = 0

Query: 7   MGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFF 66
           M  E+TL++IKPD + GN    I     +SG +I+   +++LD+ +A  FYAEH+ R F+
Sbjct: 1   MANERTLSIIKPDAVAGNNIGAIYDRFEKSGLKIVAAKMMQLDDKKAGGFYAEHAERPFY 60

Query: 67  SGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVH 126
           + LV +MTSGPV + VLE +NAIA  R ++G   AT  K     +IRA      ++N VH
Sbjct: 61  NDLVSFMTSGPVLVSVLEGENAIATHRDIMG---ATNPKDAAEGTIRADFASSIDENAVH 120

Query: 127 GSDSLQSAQREISFFFEEE 146
           GSDS +SA REIS+FF EE
Sbjct: 121 GSDSAESAAREISYFFNEE 136

BLAST of MS009606 vs. ExPASy TrEMBL
Match: A0A6J1D607 (Nucleoside diphosphate kinase OS=Momordica charantia OX=3673 GN=LOC111017349 PE=3 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.8e-76
Identity = 146/153 (95.42%), Postives = 150/153 (98.04%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNGNM TEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH
Sbjct: 27  FASNGNMVTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIYPRY 154
           EKNCVHGSDSLQSAQREISFFFEEETG++  ++
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEEETGEMVGKH 179

BLAST of MS009606 vs. ExPASy TrEMBL
Match: A0A6J1D3V3 (Nucleoside diphosphate kinase OS=Momordica charantia OX=3673 GN=LOC111017349 PE=3 SV=1)

HSP 1 Score: 292.0 bits (746), Expect = 1.5e-75
Identity = 145/146 (99.32%), Postives = 145/146 (99.32%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNGNM TEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH
Sbjct: 27  FASNGNMVTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEET 147
           EKNCVHGSDSLQSAQREISFFFEEET
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEEET 172

BLAST of MS009606 vs. ExPASy TrEMBL
Match: A0A6J1GNH4 (Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.6e-69
Identity = 135/153 (88.24%), Postives = 145/153 (94.77%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIYPRY 154
           EKNCVHGSDSLQSAQREISFFF EETG+I  ++
Sbjct: 147 EKNCVHGSDSLQSAQREISFFF-EETGEIVGKH 178

BLAST of MS009606 vs. ExPASy TrEMBL
Match: A0A6J1GQ67 (Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.6e-69
Identity = 133/150 (88.67%), Postives = 141/150 (94.00%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEEETGKIY 151
           EKNCVHGSDSLQSAQREISFFFEE    +Y
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEETGVLLY 176

BLAST of MS009606 vs. ExPASy TrEMBL
Match: A0A6J1GQ66 (Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.1e-69
Identity = 132/144 (91.67%), Postives = 139/144 (96.53%), Query Frame = 0

Query: 1   FASNGNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEH 60
           FASNG M TEKTLAMIKPDGLRGNYT+RI++AIVESGFRILKE +VELDEDRASRFYAEH
Sbjct: 27  FASNGGMETEKTLAMIKPDGLRGNYTDRIRRAIVESGFRILKERVVELDEDRASRFYAEH 86

Query: 61  SSRNFFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 120
           SS++FF  LVKYMTSGPVC+MVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS
Sbjct: 87  SSKSFFPTLVKYMTSGPVCVMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDS 146

Query: 121 EKNCVHGSDSLQSAQREISFFFEE 145
           EKNCVHGSDSLQSAQREISFFFEE
Sbjct: 147 EKNCVHGSDSLQSAQREISFFFEE 170

BLAST of MS009606 vs. TAIR 10
Match: AT1G17410.1 (Nucleoside diphosphate kinase family protein )

HSP 1 Score: 198.0 bits (502), Expect = 5.7e-51
Identity = 91/140 (65.00%), Postives = 115/140 (82.14%), Query Frame = 0

Query: 5   GNMGTEKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRN 64
           G    E+TLAMIKPDG+ GNYT  IK  +VE+GF I+KE++ +LD++ AS FY EHSSR+
Sbjct: 28  GASSEERTLAMIKPDGVSGNYTEEIKTIVVEAGFNIVKEMLTQLDKETASAFYEEHSSRS 87

Query: 65  FFSGLVKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNC 124
           FF  LV YMTSGPV +MVLEK+NA++DWR +IGPTDA KAK +HP+SIRA+CG +S+KNC
Sbjct: 88  FFPHLVTYMTSGPVLVMVLEKRNAVSDWRDLIGPTDAEKAKISHPHSIRALCGKNSQKNC 147

Query: 125 VHGSDSLQSAQREISFFFEE 145
           VHGSDS  SA+REI FFF++
Sbjct: 148 VHGSDSTSSAEREIKFFFKD 167

BLAST of MS009606 vs. TAIR 10
Match: AT1G17410.2 (Nucleoside diphosphate kinase family protein )

HSP 1 Score: 189.9 bits (481), Expect = 1.6e-48
Identity = 86/130 (66.15%), Postives = 109/130 (83.85%), Query Frame = 0

Query: 15  MIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGLVKYMT 74
           MIKPDG+ GNYT  IK  +VE+GF I+KE++ +LD++ AS FY EHSSR+FF  LV YMT
Sbjct: 1   MIKPDGVSGNYTEEIKTIVVEAGFNIVKEMLTQLDKETASAFYEEHSSRSFFPHLVTYMT 60

Query: 75  SGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSDSLQSA 134
           SGPV +MVLEK+NA++DWR +IGPTDA KAK +HP+SIRA+CG +S+KNCVHGSDS  SA
Sbjct: 61  SGPVLVMVLEKRNAVSDWRDLIGPTDAEKAKISHPHSIRALCGKNSQKNCVHGSDSTSSA 120

Query: 135 QREISFFFEE 145
           +REI FFF++
Sbjct: 121 EREIKFFFKD 130

BLAST of MS009606 vs. TAIR 10
Match: AT4G09320.1 (Nucleoside diphosphate kinase family protein )

HSP 1 Score: 95.9 bits (237), Expect = 3.1e-20
Identity = 49/135 (36.30%), Postives = 80/135 (59.26%), Query Frame = 0

Query: 10  EKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGL 69
           E+T  MIKPDG++      +     + GF +    ++ ++   A + Y + SS++FFSGL
Sbjct: 22  EQTFIMIKPDGVQRGLIGEVICRFEKKGFTLKGLKLISVERSFAEKHYEDLSSKSFFSGL 81

Query: 70  VKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSD 129
           V Y+ SGPV  M+ E +N +   R +IG   AT   A+ P +IR    +D  +N +HGSD
Sbjct: 82  VDYIVSGPVVAMIWEGKNVVLTGRKIIG---ATNPAASEPGTIRGDFAIDIGRNVIHGSD 141

Query: 130 SLQSAQREISFFFEE 145
           S++SA++EI+ +F +
Sbjct: 142 SVESARKEIALWFPD 153

BLAST of MS009606 vs. TAIR 10
Match: AT4G11010.1 (nucleoside diphosphate kinase 3 )

HSP 1 Score: 82.4 bits (202), Expect = 3.5e-16
Identity = 45/136 (33.09%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 10  EKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGL 69
           E+T   IKPDG++    + I       GF+++   ++   +D A + Y +   R FF+GL
Sbjct: 89  ERTFIAIKPDGVQRGLISEIISRFERKGFKLVGIKVIVPSKDFAQKHYHDLKERPFFNGL 148

Query: 70  VKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSD 129
             +++SGPV  MV E    I   R +IG TD  K++   P +IR    +   +N +HGSD
Sbjct: 149 CDFLSSGPVIAMVWEGDGVIRYGRKLIGATDPQKSE---PGTIRGDLAVTVGRNIIHGSD 208

Query: 130 SLQSAQREISFFFEEE 146
             ++A+ EIS +F+ +
Sbjct: 209 GPETAKDEISLWFKPQ 221

BLAST of MS009606 vs. TAIR 10
Match: AT4G23900.1 (Nucleoside diphosphate kinase family protein )

HSP 1 Score: 79.0 bits (193), Expect = 3.9e-15
Identity = 44/136 (32.35%), Postives = 74/136 (54.41%), Query Frame = 0

Query: 10  EKTLAMIKPDGLRGNYTNRIKKAIVESGFRILKEIIVELDEDRASRFYAEHSSRNFFSGL 69
           E+T   IKPDG++    + I       G++++   ++   +  A + Y +   R FF+GL
Sbjct: 88  ERTFIAIKPDGVQRGLISEIITRFERKGYKLVGIKVMVPSKGFAQKHYHDLKERPFFNGL 147

Query: 70  VKYMTSGPVCIMVLEKQNAIADWRAMIGPTDATKAKATHPNSIRAMCGLDSEKNCVHGSD 129
             +++SGPV  MV E +  I   R +IG TD  K++   P +IR    +   +N +HGSD
Sbjct: 148 CNFLSSGPVVAMVWEGEGVIRYGRKLIGATDPQKSE---PGTIRGDLAVVVGRNIIHGSD 207

Query: 130 SLQSAQREISFFFEEE 146
             ++A+ EIS +F+ E
Sbjct: 208 GPETAKDEISLWFKPE 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148787.13.7e-7695.42probable nucleoside diphosphate kinase 5 isoform X2 [Momordica charantia][more]
XP_022148770.13.1e-7599.32probable nucleoside diphosphate kinase 5 isoform X1 [Momordica charantia] >XP_02... [more]
XP_022953616.13.3e-6988.24probable nucleoside diphosphate kinase 5 isoform X3 [Cucurbita moschata][more]
XP_022953615.17.4e-6988.67probable nucleoside diphosphate kinase 5 isoform X2 [Cucurbita moschata][more]
XP_023547543.17.4e-6987.58probable nucleoside diphosphate kinase 5 isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q6NLG32.2e-4766.15Probable nucleoside diphosphate kinase 5 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
A5FY401.1e-2748.53Nucleoside diphosphate kinase OS=Acidiphilium cryptum (strain JF-5) OX=349163 GN... [more]
A6VMK71.6e-2644.52Nucleoside diphosphate kinase OS=Actinobacillus succinogenes (strain ATCC 55618 ... [more]
B0USF11.6e-2641.78Nucleoside diphosphate kinase OS=Histophilus somni (strain 2336) OX=228400 GN=nd... [more]
Q1QD231.6e-2646.04Nucleoside diphosphate kinase OS=Psychrobacter cryohalolentis (strain ATCC BAA-1... [more]
Match NameE-valueIdentityDescription
A0A6J1D6071.8e-7695.42Nucleoside diphosphate kinase OS=Momordica charantia OX=3673 GN=LOC111017349 PE=... [more]
A0A6J1D3V31.5e-7599.32Nucleoside diphosphate kinase OS=Momordica charantia OX=3673 GN=LOC111017349 PE=... [more]
A0A6J1GNH41.6e-6988.24Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3... [more]
A0A6J1GQ673.6e-6988.67Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3... [more]
A0A6J1GQ666.1e-6991.67Nucleoside diphosphate kinase OS=Cucurbita moschata OX=3662 GN=LOC111456097 PE=3... [more]
Match NameE-valueIdentityDescription
AT1G17410.15.7e-5165.00Nucleoside diphosphate kinase family protein [more]
AT1G17410.21.6e-4866.15Nucleoside diphosphate kinase family protein [more]
AT4G09320.13.1e-2036.30Nucleoside diphosphate kinase family protein [more]
AT4G11010.13.5e-1633.09nucleoside diphosphate kinase 3 [more]
AT4G23900.13.9e-1532.35Nucleoside diphosphate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (TR) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001564Nucleoside diphosphate kinasePRINTSPR01243NUCDPKINASEcoord: 12..34
score: 31.49
coord: 76..93
score: 42.74
coord: 123..142
score: 58.23
coord: 56..75
score: 48.71
IPR034907Nucleoside diphosphate kinase-like domainSMARTSM00562ndk_5coord: 9..149
e-value: 2.0E-60
score: 216.7
IPR034907Nucleoside diphosphate kinase-like domainPFAMPF00334NDKcoord: 10..145
e-value: 6.7E-45
score: 152.4
IPR036850Nucleoside diphosphate kinase-like domain superfamilyGENE3D3.30.70.141coord: 5..152
e-value: 3.8E-53
score: 181.5
IPR036850Nucleoside diphosphate kinase-like domain superfamilySUPERFAMILY54919Nucleoside diphosphate kinase, NDKcoord: 7..145
NoneNo IPR availablePANTHERPTHR46161NUCLEOSIDE DIPHOSPHATE KINASEcoord: 4..144
NoneNo IPR availablePANTHERPTHR46161:SF3NUCLEOSIDE DIPHOSPHATE KINASEcoord: 4..144
IPR023005Nucleoside diphosphate kinase, active sitePROSITEPS00469NDP_KINASEScoord: 123..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
MS009606.1MS009606.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006241 CTP biosynthetic process
biological_process GO:0006183 GTP biosynthetic process
biological_process GO:0006165 nucleoside diphosphate phosphorylation
biological_process GO:0006228 UTP biosynthetic process
molecular_function GO:0004550 nucleoside diphosphate kinase activity